Carg08460 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg08460
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionxyloglucan galactosyltransferase XLT2-like
LocationCarg_Chr02: 5566247 .. 5567737 (+)
RNA-Seq ExpressionCarg08460
SyntenyCarg08460
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTTCCTCTTTCCGGCAACTCCTCGCCGGAGCATCATCCGAGGAAATTGAAATCCTACGAGTTGTTCGGTAGAAAGAACTCGTTCAACTCGTTGTTACAGCAATTTCATCATCAACAATCTCGTCTATGGCTTCTCCTCACAATCCTTTGCCTCCAGATCCTCCTCCTCTTCATTATTCGCTTCCTTTCTCTTCCTCTTCCTCTTCCTCTCCCGCCGGCAATCTCCTCTGCCGCCAACCGCCTTCATCGCTTCCCCTCCGCTGCCGTCTCTCCCGCCTCTACCGACGGCGATCACTGCAAAAATGGCCGGATCTTCGTCTACGACCTGCCGACGGCATTTAACGAAGACATTGTCGAAAACTGCGATAGTCTGAACCCGTGGAGTTCAAGTTGCAGCGCGATGGTGAACGGCGGGTTCGGGCAGAGAGCTGATTCTCTCGCCGGAGTGATACCGGAGAATCTCTTGGCGTCGTGGTATTGGACGGACCAATTCGTAACGGAAATCATTTTCCATAATCGGATAATGAAGCATAAATGCCGTGTCAAGGAGCCGGAATCTGCTACGGCGTTTTATGTACCGTTTTACGCTGGACTCGCAGTGGGGAAATTCCTATGGACGGCTTCGAGCGCGGAGGATCGGGATCATCACTGCCGTATGATTCTAAGGTGGCTTTCCGATCAGGAGTACTATAAGAAATCAAACGGCTGGGATCATTTCATTACAATGGGCCGCATCACATGGGATTTCCGCCGCAGCAGTGATGAGGATTGGGGGTCGAGGTGTATTTATTTACCGGGAATGAGAAATATTACTCGCCTTCTGATAGAGCGAAATCCGTGGGACTATTTTGACGTCGGTGTACCTTACCCCACAGGATTCCACCCCAGTAGCCAGGACGACATGTCGGCCTGGCAGGACTTCGTCCGCACGCGCAGTCGTACGCACCTCTTCTGCTTCGCTGGCGCCAAACGCGCCGCCTTCCGTAATGACTTTAGGGGGATCCTGCTGGATCAGTGCCGGAATTCCACGGCGGAGAAATGCCGCGTTGTCGACTGTGCCGGTAGCCGCTGCTCCAATGGCACGTCGGCGATACTCGAATCGTTTCTCAATTCGGATTTCTGCTTGCAGCCAAGAGGCGACAGCTTCACGCGGCGGTCGATCTTCGACTGTATGGTGGCCGGAGCGATTCCAGTGTTCTTCTGGCGGCGGACAGCGTATTACCAGTACGAGTGGTTCTTACCAGGCAAACCGGAGAGTTACTCGGTTTTCATAGACCGGAACGCCGTGAAGAACGGGACCGCCTCCATTGAAGCGGTTCTGGAGAAATTTAGCAGAGAAGAAGTGAGAGCGATGAGAGATAGAGTAATCGAGTCGATACCGAAGTTCGTTTACGGCACCGGCGCTGTCAGAGATGCCTTCGACGTGGCCGTCGATGGGGTTTTGAGGAGGTTTAAAGAGCAAGAAGAATGGGGGTACAAGTGGAAATAA

mRNA sequence

ATGCTTCCTCTTTCCGGCAACTCCTCGCCGGAGCATCATCCGAGGAAATTGAAATCCTACGAGTTGTTCGGTAGAAAGAACTCGTTCAACTCGTTGTTACAGCAATTTCATCATCAACAATCTCGTCTATGGCTTCTCCTCACAATCCTTTGCCTCCAGATCCTCCTCCTCTTCATTATTCGCTTCCTTTCTCTTCCTCTTCCTCTTCCTCTCCCGCCGGCAATCTCCTCTGCCGCCAACCGCCTTCATCGCTTCCCCTCCGCTGCCGTCTCTCCCGCCTCTACCGACGGCGATCACTGCAAAAATGGCCGGATCTTCGTCTACGACCTGCCGACGGCATTTAACGAAGACATTGTCGAAAACTGCGATAGTCTGAACCCGTGGAGTTCAAGTTGCAGCGCGATGGTGAACGGCGGGTTCGGGCAGAGAGCTGATTCTCTCGCCGGAGTGATACCGGAGAATCTCTTGGCGTCGTGGTATTGGACGGACCAATTCGTAACGGAAATCATTTTCCATAATCGGATAATGAAGCATAAATGCCGTGTCAAGGAGCCGGAATCTGCTACGGCGTTTTATGTACCGTTTTACGCTGGACTCGCAGTGGGGAAATTCCTATGGACGGCTTCGAGCGCGGAGGATCGGGATCATCACTGCCGTATGATTCTAAGGTGGCTTTCCGATCAGGAGTACTATAAGAAATCAAACGGCTGGGATCATTTCATTACAATGGGCCGCATCACATGGGATTTCCGCCGCAGCAGTGATGAGGATTGGGGGTCGAGGTGTATTTATTTACCGGGAATGAGAAATATTACTCGCCTTCTGATAGAGCGAAATCCGTGGGACTATTTTGACGTCGGTGTACCTTACCCCACAGGATTCCACCCCAGTAGCCAGGACGACATGTCGGCCTGGCAGGACTTCGTCCGCACGCGCAGTCGTACGCACCTCTTCTGCTTCGCTGGCGCCAAACGCGCCGCCTTCCGTAATGACTTTAGGGGGATCCTGCTGGATCAGTGCCGGAATTCCACGGCGGAGAAATGCCGCGTTGTCGACTGTGCCGGTAGCCGCTGCTCCAATGGCACGTCGGCGATACTCGAATCGTTTCTCAATTCGGATTTCTGCTTGCAGCCAAGAGGCGACAGCTTCACGCGGCGGTCGATCTTCGACTGTATGGTGGCCGGAGCGATTCCAGTGTTCTTCTGGCGGCGGACAGCGTATTACCAGTACGAGTGGTTCTTACCAGGCAAACCGGAGAGTTACTCGGTTTTCATAGACCGGAACGCCGTGAAGAACGGGACCGCCTCCATTGAAGCGGTTCTGGAGAAATTTAGCAGAGAAGAAGTGAGAGCGATGAGAGATAGAGTAATCGAGTCGATACCGAAGTTCGTTTACGGCACCGGCGCTGTCAGAGATGCCTTCGACGTGGCCGTCGATGGGGTTTTGAGGAGGTTTAAAGAGCAAGAAGAATGGGGGTACAAGTGGAAATAA

Coding sequence (CDS)

ATGCTTCCTCTTTCCGGCAACTCCTCGCCGGAGCATCATCCGAGGAAATTGAAATCCTACGAGTTGTTCGGTAGAAAGAACTCGTTCAACTCGTTGTTACAGCAATTTCATCATCAACAATCTCGTCTATGGCTTCTCCTCACAATCCTTTGCCTCCAGATCCTCCTCCTCTTCATTATTCGCTTCCTTTCTCTTCCTCTTCCTCTTCCTCTCCCGCCGGCAATCTCCTCTGCCGCCAACCGCCTTCATCGCTTCCCCTCCGCTGCCGTCTCTCCCGCCTCTACCGACGGCGATCACTGCAAAAATGGCCGGATCTTCGTCTACGACCTGCCGACGGCATTTAACGAAGACATTGTCGAAAACTGCGATAGTCTGAACCCGTGGAGTTCAAGTTGCAGCGCGATGGTGAACGGCGGGTTCGGGCAGAGAGCTGATTCTCTCGCCGGAGTGATACCGGAGAATCTCTTGGCGTCGTGGTATTGGACGGACCAATTCGTAACGGAAATCATTTTCCATAATCGGATAATGAAGCATAAATGCCGTGTCAAGGAGCCGGAATCTGCTACGGCGTTTTATGTACCGTTTTACGCTGGACTCGCAGTGGGGAAATTCCTATGGACGGCTTCGAGCGCGGAGGATCGGGATCATCACTGCCGTATGATTCTAAGGTGGCTTTCCGATCAGGAGTACTATAAGAAATCAAACGGCTGGGATCATTTCATTACAATGGGCCGCATCACATGGGATTTCCGCCGCAGCAGTGATGAGGATTGGGGGTCGAGGTGTATTTATTTACCGGGAATGAGAAATATTACTCGCCTTCTGATAGAGCGAAATCCGTGGGACTATTTTGACGTCGGTGTACCTTACCCCACAGGATTCCACCCCAGTAGCCAGGACGACATGTCGGCCTGGCAGGACTTCGTCCGCACGCGCAGTCGTACGCACCTCTTCTGCTTCGCTGGCGCCAAACGCGCCGCCTTCCGTAATGACTTTAGGGGGATCCTGCTGGATCAGTGCCGGAATTCCACGGCGGAGAAATGCCGCGTTGTCGACTGTGCCGGTAGCCGCTGCTCCAATGGCACGTCGGCGATACTCGAATCGTTTCTCAATTCGGATTTCTGCTTGCAGCCAAGAGGCGACAGCTTCACGCGGCGGTCGATCTTCGACTGTATGGTGGCCGGAGCGATTCCAGTGTTCTTCTGGCGGCGGACAGCGTATTACCAGTACGAGTGGTTCTTACCAGGCAAACCGGAGAGTTACTCGGTTTTCATAGACCGGAACGCCGTGAAGAACGGGACCGCCTCCATTGAAGCGGTTCTGGAGAAATTTAGCAGAGAAGAAGTGAGAGCGATGAGAGATAGAGTAATCGAGTCGATACCGAAGTTCGTTTACGGCACCGGCGCTGTCAGAGATGCCTTCGACGTGGCCGTCGATGGGGTTTTGAGGAGGTTTAAAGAGCAAGAAGAATGGGGGTACAAGTGGAAATAA

Protein sequence

MLPLSGNSSPEHHPRKLKSYELFGRKNSFNSLLQQFHHQQSRLWLLLTILCLQILLLFIIRFLSLPLPLPLPPAISSAANRLHRFPSAAVSPASTDGDHCKNGRIFVYDLPTAFNEDIVENCDSLNPWSSSCSAMVNGGFGQRADSLAGVIPENLLASWYWTDQFVTEIIFHNRIMKHKCRVKEPESATAFYVPFYAGLAVGKFLWTASSAEDRDHHCRMILRWLSDQEYYKKSNGWDHFITMGRITWDFRRSSDEDWGSRCIYLPGMRNITRLLIERNPWDYFDVGVPYPTGFHPSSQDDMSAWQDFVRTRSRTHLFCFAGAKRAAFRNDFRGILLDQCRNSTAEKCRVVDCAGSRCSNGTSAILESFLNSDFCLQPRGDSFTRRSIFDCMVAGAIPVFFWRRTAYYQYEWFLPGKPESYSVFIDRNAVKNGTASIEAVLEKFSREEVRAMRDRVIESIPKFVYGTGAVRDAFDVAVDGVLRRFKEQEEWGYKWK
Homology
BLAST of Carg08460 vs. NCBI nr
Match: KAG7035587.1 (Xyloglucan galactosyltransferase XLT2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1038.9 bits (2685), Expect = 1.4e-299
Identity = 496/496 (100.00%), Postives = 496/496 (100.00%), Query Frame = 0

Query: 1   MLPLSGNSSPEHHPRKLKSYELFGRKNSFNSLLQQFHHQQSRLWLLLTILCLQILLLFII 60
           MLPLSGNSSPEHHPRKLKSYELFGRKNSFNSLLQQFHHQQSRLWLLLTILCLQILLLFII
Sbjct: 1   MLPLSGNSSPEHHPRKLKSYELFGRKNSFNSLLQQFHHQQSRLWLLLTILCLQILLLFII 60

Query: 61  RFLSLPLPLPLPPAISSAANRLHRFPSAAVSPASTDGDHCKNGRIFVYDLPTAFNEDIVE 120
           RFLSLPLPLPLPPAISSAANRLHRFPSAAVSPASTDGDHCKNGRIFVYDLPTAFNEDIVE
Sbjct: 61  RFLSLPLPLPLPPAISSAANRLHRFPSAAVSPASTDGDHCKNGRIFVYDLPTAFNEDIVE 120

Query: 121 NCDSLNPWSSSCSAMVNGGFGQRADSLAGVIPENLLASWYWTDQFVTEIIFHNRIMKHKC 180
           NCDSLNPWSSSCSAMVNGGFGQRADSLAGVIPENLLASWYWTDQFVTEIIFHNRIMKHKC
Sbjct: 121 NCDSLNPWSSSCSAMVNGGFGQRADSLAGVIPENLLASWYWTDQFVTEIIFHNRIMKHKC 180

Query: 181 RVKEPESATAFYVPFYAGLAVGKFLWTASSAEDRDHHCRMILRWLSDQEYYKKSNGWDHF 240
           RVKEPESATAFYVPFYAGLAVGKFLWTASSAEDRDHHCRMILRWLSDQEYYKKSNGWDHF
Sbjct: 181 RVKEPESATAFYVPFYAGLAVGKFLWTASSAEDRDHHCRMILRWLSDQEYYKKSNGWDHF 240

Query: 241 ITMGRITWDFRRSSDEDWGSRCIYLPGMRNITRLLIERNPWDYFDVGVPYPTGFHPSSQD 300
           ITMGRITWDFRRSSDEDWGSRCIYLPGMRNITRLLIERNPWDYFDVGVPYPTGFHPSSQD
Sbjct: 241 ITMGRITWDFRRSSDEDWGSRCIYLPGMRNITRLLIERNPWDYFDVGVPYPTGFHPSSQD 300

Query: 301 DMSAWQDFVRTRSRTHLFCFAGAKRAAFRNDFRGILLDQCRNSTAEKCRVVDCAGSRCSN 360
           DMSAWQDFVRTRSRTHLFCFAGAKRAAFRNDFRGILLDQCRNSTAEKCRVVDCAGSRCSN
Sbjct: 301 DMSAWQDFVRTRSRTHLFCFAGAKRAAFRNDFRGILLDQCRNSTAEKCRVVDCAGSRCSN 360

Query: 361 GTSAILESFLNSDFCLQPRGDSFTRRSIFDCMVAGAIPVFFWRRTAYYQYEWFLPGKPES 420
           GTSAILESFLNSDFCLQPRGDSFTRRSIFDCMVAGAIPVFFWRRTAYYQYEWFLPGKPES
Sbjct: 361 GTSAILESFLNSDFCLQPRGDSFTRRSIFDCMVAGAIPVFFWRRTAYYQYEWFLPGKPES 420

Query: 421 YSVFIDRNAVKNGTASIEAVLEKFSREEVRAMRDRVIESIPKFVYGTGAVRDAFDVAVDG 480
           YSVFIDRNAVKNGTASIEAVLEKFSREEVRAMRDRVIESIPKFVYGTGAVRDAFDVAVDG
Sbjct: 421 YSVFIDRNAVKNGTASIEAVLEKFSREEVRAMRDRVIESIPKFVYGTGAVRDAFDVAVDG 480

Query: 481 VLRRFKEQEEWGYKWK 497
           VLRRFKEQEEWGYKWK
Sbjct: 481 VLRRFKEQEEWGYKWK 496

BLAST of Carg08460 vs. NCBI nr
Match: KAG6605679.1 (Xyloglucan galactosyltransferase XLT2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1036.9 bits (2680), Expect = 5.4e-299
Identity = 495/496 (99.80%), Postives = 495/496 (99.80%), Query Frame = 0

Query: 1   MLPLSGNSSPEHHPRKLKSYELFGRKNSFNSLLQQFHHQQSRLWLLLTILCLQILLLFII 60
           MLPLSGNSSPEHHPRKLKSYELFGRKNSFNSLLQQFHHQQSRLWLLLTILCLQILLLFII
Sbjct: 1   MLPLSGNSSPEHHPRKLKSYELFGRKNSFNSLLQQFHHQQSRLWLLLTILCLQILLLFII 60

Query: 61  RFLSLPLPLPLPPAISSAANRLHRFPSAAVSPASTDGDHCKNGRIFVYDLPTAFNEDIVE 120
           RFLSLPLPLPLPPAISSAANRLHRFPSAAVSPASTDGDHCKNGRIFVYDLPTAFNEDIVE
Sbjct: 61  RFLSLPLPLPLPPAISSAANRLHRFPSAAVSPASTDGDHCKNGRIFVYDLPTAFNEDIVE 120

Query: 121 NCDSLNPWSSSCSAMVNGGFGQRADSLAGVIPENLLASWYWTDQFVTEIIFHNRIMKHKC 180
           NCDSLNPWSSSCSAMVNGGFGQRADSLAGVIPENLLASWYWTDQFVTEIIFHNRIMKHKC
Sbjct: 121 NCDSLNPWSSSCSAMVNGGFGQRADSLAGVIPENLLASWYWTDQFVTEIIFHNRIMKHKC 180

Query: 181 RVKEPESATAFYVPFYAGLAVGKFLWTASSAEDRDHHCRMILRWLSDQEYYKKSNGWDHF 240
           RVKEPESATAFYVPFYAGLAVGKFLWTASSAEDRDHHCRMILRWLSDQEYYKKSNGWDHF
Sbjct: 181 RVKEPESATAFYVPFYAGLAVGKFLWTASSAEDRDHHCRMILRWLSDQEYYKKSNGWDHF 240

Query: 241 ITMGRITWDFRRSSDEDWGSRCIYLPGMRNITRLLIERNPWDYFDVGVPYPTGFHPSSQD 300
           ITMGRITWDFRRSSDEDWGSRCIYLPGMRNITRLLIERNPWDYFDVGVPYPTGFHPSSQD
Sbjct: 241 ITMGRITWDFRRSSDEDWGSRCIYLPGMRNITRLLIERNPWDYFDVGVPYPTGFHPSSQD 300

Query: 301 DMSAWQDFVRTRSRTHLFCFAGAKRAAFRNDFRGILLDQCRNSTAEKCRVVDCAGSRCSN 360
           DMSAWQDFVRTRSRTHLFCFAGAKRAAFRNDFRGILLDQCRNSTAEKCRVVDCAGSRCSN
Sbjct: 301 DMSAWQDFVRTRSRTHLFCFAGAKRAAFRNDFRGILLDQCRNSTAEKCRVVDCAGSRCSN 360

Query: 361 GTSAILESFLNSDFCLQPRGDSFTRRSIFDCMVAGAIPVFFWRRTAYYQYEWFLPGKPES 420
           GTSAILESFLNSDFCLQPRGDSFTRRSIFDCMVAGAIPVFFWRRTAYYQYEWFLPGKPES
Sbjct: 361 GTSAILESFLNSDFCLQPRGDSFTRRSIFDCMVAGAIPVFFWRRTAYYQYEWFLPGKPES 420

Query: 421 YSVFIDRNAVKNGTASIEAVLEKFSREEVRAMRDRVIESIPKFVYGTGAVRDAFDVAVDG 480
           YSVFIDRNAVKNGTASIEAVLEKFSREEVR MRDRVIESIPKFVYGTGAVRDAFDVAVDG
Sbjct: 421 YSVFIDRNAVKNGTASIEAVLEKFSREEVREMRDRVIESIPKFVYGTGAVRDAFDVAVDG 480

Query: 481 VLRRFKEQEEWGYKWK 497
           VLRRFKEQEEWGYKWK
Sbjct: 481 VLRRFKEQEEWGYKWK 496

BLAST of Carg08460 vs. NCBI nr
Match: XP_023532626.1 (xyloglucan galactosyltransferase XLT2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1031.2 bits (2665), Expect = 3.0e-297
Identity = 491/496 (98.99%), Postives = 494/496 (99.60%), Query Frame = 0

Query: 1   MLPLSGNSSPEHHPRKLKSYELFGRKNSFNSLLQQFHHQQSRLWLLLTILCLQILLLFII 60
           MLPLSGNSSPEHHPRKLKSYELFGRKNSFNSLLQQFHHQQSRLWLLLTILCLQILLLFII
Sbjct: 1   MLPLSGNSSPEHHPRKLKSYELFGRKNSFNSLLQQFHHQQSRLWLLLTILCLQILLLFII 60

Query: 61  RFLSLPLPLPLPPAISSAANRLHRFPSAAVSPASTDGDHCKNGRIFVYDLPTAFNEDIVE 120
           RFLSLPLPLPLPPAISSAANRLHRFPSAAVSPASTDGDHCKNGRIFVYDLPTAFN+DIV 
Sbjct: 61  RFLSLPLPLPLPPAISSAANRLHRFPSAAVSPASTDGDHCKNGRIFVYDLPTAFNQDIVN 120

Query: 121 NCDSLNPWSSSCSAMVNGGFGQRADSLAGVIPENLLASWYWTDQFVTEIIFHNRIMKHKC 180
           NCDSLNPWSSSCSAMVNGGFGQRADSLAGVIPENLLASWYWTDQFVTEIIFHNRIMKHKC
Sbjct: 121 NCDSLNPWSSSCSAMVNGGFGQRADSLAGVIPENLLASWYWTDQFVTEIIFHNRIMKHKC 180

Query: 181 RVKEPESATAFYVPFYAGLAVGKFLWTASSAEDRDHHCRMILRWLSDQEYYKKSNGWDHF 240
           RVKEPESATAFYVPFYAGLAVGKFLWTASSAEDRDHHCRMIL+WLSDQEYYKKSNGWDHF
Sbjct: 181 RVKEPESATAFYVPFYAGLAVGKFLWTASSAEDRDHHCRMILKWLSDQEYYKKSNGWDHF 240

Query: 241 ITMGRITWDFRRSSDEDWGSRCIYLPGMRNITRLLIERNPWDYFDVGVPYPTGFHPSSQD 300
           ITMGRITWDFRRSSDEDWGSRCIYLPGMRNITRLLIERNPWDYFDVGVPYPTGFHPSSQD
Sbjct: 241 ITMGRITWDFRRSSDEDWGSRCIYLPGMRNITRLLIERNPWDYFDVGVPYPTGFHPSSQD 300

Query: 301 DMSAWQDFVRTRSRTHLFCFAGAKRAAFRNDFRGILLDQCRNSTAEKCRVVDCAGSRCSN 360
           DMSAWQDFVRTRSRTHLFCFAGAKRAAFRNDFRGILLDQCRNSTAEKCRVVDCAGSRCSN
Sbjct: 301 DMSAWQDFVRTRSRTHLFCFAGAKRAAFRNDFRGILLDQCRNSTAEKCRVVDCAGSRCSN 360

Query: 361 GTSAILESFLNSDFCLQPRGDSFTRRSIFDCMVAGAIPVFFWRRTAYYQYEWFLPGKPES 420
           GTSAILESFLNSDFCLQPRGDSFTRRSIFDCMVAGAIPVFFWRRTAYYQYEWFLPGKPES
Sbjct: 361 GTSAILESFLNSDFCLQPRGDSFTRRSIFDCMVAGAIPVFFWRRTAYYQYEWFLPGKPES 420

Query: 421 YSVFIDRNAVKNGTASIEAVLEKFSREEVRAMRDRVIESIPKFVYGTGAVRDAFDVAVDG 480
           YSVFIDRNAVKNGTASIEAVLEKFSREEVR MR+RVIESIPKFVYGTGAVRDAFDVAVDG
Sbjct: 421 YSVFIDRNAVKNGTASIEAVLEKFSREEVREMRERVIESIPKFVYGTGAVRDAFDVAVDG 480

Query: 481 VLRRFKEQEEWGYKWK 497
           VLRRFKEQEEWGYKWK
Sbjct: 481 VLRRFKEQEEWGYKWK 496

BLAST of Carg08460 vs. NCBI nr
Match: XP_022957641.1 (xyloglucan galactosyltransferase XLT2-like [Cucurbita moschata])

HSP 1 Score: 1020.4 bits (2637), Expect = 5.3e-294
Identity = 487/496 (98.19%), Postives = 490/496 (98.79%), Query Frame = 0

Query: 1   MLPLSGNSSPEHHPRKLKSYELFGRKNSFNSLLQQFHHQQSRLWLLLTILCLQILLLFII 60
           MLPLSGNSSPEHHPRKLKSYELFGRKNSFNSLLQQFHHQQS LWLLLTILCLQILLLFII
Sbjct: 1   MLPLSGNSSPEHHPRKLKSYELFGRKNSFNSLLQQFHHQQSCLWLLLTILCLQILLLFII 60

Query: 61  RFLSLPLPLPLPPAISSAANRLHRFPSAAVSPASTDGDHCKNGRIFVYDLPTAFNEDIVE 120
           RFLSLPLPLPLPPAISSAANRLHRFPSAAVSPASTDGDHCKNGRIFVYDLPT FN+DIV+
Sbjct: 61  RFLSLPLPLPLPPAISSAANRLHRFPSAAVSPASTDGDHCKNGRIFVYDLPTEFNQDIVQ 120

Query: 121 NCDSLNPWSSSCSAMVNGGFGQRADSLAGVIPENLLASWYWTDQFVTEIIFHNRIMKHKC 180
           NCDSLNPWSSSCSAMVNGGFGQRADSLAGVIPENLLASWYWTDQFVTEIIFHNRIMKHKC
Sbjct: 121 NCDSLNPWSSSCSAMVNGGFGQRADSLAGVIPENLLASWYWTDQFVTEIIFHNRIMKHKC 180

Query: 181 RVKEPESATAFYVPFYAGLAVGKFLWTASSAEDRDHHCRMILRWLSDQEYYKKSNGWDHF 240
           RVKEPESATAFYVPFYAGLAVGKFLWTASSAE RDHHCRMILRWLSDQEYYKKSNGWDHF
Sbjct: 181 RVKEPESATAFYVPFYAGLAVGKFLWTASSAEARDHHCRMILRWLSDQEYYKKSNGWDHF 240

Query: 241 ITMGRITWDFRRSSDEDWGSRCIYLPGMRNITRLLIERNPWDYFDVGVPYPTGFHPSSQD 300
           ITMGRITWDFRRSSDEDWGSRCIYLPGMRNITRLLIERNPWDYFDVGVPYPTGFHPSSQD
Sbjct: 241 ITMGRITWDFRRSSDEDWGSRCIYLPGMRNITRLLIERNPWDYFDVGVPYPTGFHPSSQD 300

Query: 301 DMSAWQDFVRTRSRTHLFCFAGAKRAAFRNDFRGILLDQCRNSTAEKCRVVDCAGSRCSN 360
           DMSAWQDFVRTRSRTHLFCFAGAKRAAFRNDFRGILLDQCRNSTAEKCRVVDCAGSRCSN
Sbjct: 301 DMSAWQDFVRTRSRTHLFCFAGAKRAAFRNDFRGILLDQCRNSTAEKCRVVDCAGSRCSN 360

Query: 361 GTSAILESFLNSDFCLQPRGDSFTRRSIFDCMVAGAIPVFFWRRTAYYQYEWFLPGKPES 420
           GTSAILESFLNSDFCLQPRGDSFTRRSIFDCMVAGAIPVFFWRRTAYYQYEWFLPGKPES
Sbjct: 361 GTSAILESFLNSDFCLQPRGDSFTRRSIFDCMVAGAIPVFFWRRTAYYQYEWFLPGKPES 420

Query: 421 YSVFIDRNAVKNGTASIEAVLEKFSREEVRAMRDRVIESIPKFVYGTGAVRDAFDVAVDG 480
           YSVFIDRNAVKNGT SIEAVLEKFSREEVR MR+RVIESIPKFVYGTGAVRDAFDVAVD 
Sbjct: 421 YSVFIDRNAVKNGTTSIEAVLEKFSREEVREMRERVIESIPKFVYGTGAVRDAFDVAVDR 480

Query: 481 VLRRFKEQEEWGYKWK 497
           VLRRFKEQEEWGYKWK
Sbjct: 481 VLRRFKEQEEWGYKWK 496

BLAST of Carg08460 vs. NCBI nr
Match: XP_022996283.1 (xyloglucan galactosyltransferase XLT2-like [Cucurbita maxima])

HSP 1 Score: 1006.5 bits (2601), Expect = 7.9e-290
Identity = 480/496 (96.77%), Postives = 488/496 (98.39%), Query Frame = 0

Query: 1   MLPLSGNSSPEHHPRKLKSYELFGRKNSFNSLLQQFHHQQSRLWLLLTILCLQILLLFII 60
           MLPLSGNSSPEHHPRKLKSYELFGRKNSFNSLLQQFHHQQSRLWLLLTILCLQILL+FII
Sbjct: 1   MLPLSGNSSPEHHPRKLKSYELFGRKNSFNSLLQQFHHQQSRLWLLLTILCLQILLIFII 60

Query: 61  RFLSLPLPLPLPPAISSAANRLHRFPSAAVSPASTDGDHCKNGRIFVYDLPTAFNEDIVE 120
           RFLS   PLPLPP ISSAANRL+RFPSAAVSPASTD DHCKNGRIFVYDLPTAFN+DIV+
Sbjct: 61  RFLS--FPLPLPPVISSAANRLYRFPSAAVSPASTDVDHCKNGRIFVYDLPTAFNQDIVK 120

Query: 121 NCDSLNPWSSSCSAMVNGGFGQRADSLAGVIPENLLASWYWTDQFVTEIIFHNRIMKHKC 180
           NCDSLNPWSSSCSAMVNGGFGQRADSLAGVIPENL+ASWYWTDQFVTEIIFHNRIMKHKC
Sbjct: 121 NCDSLNPWSSSCSAMVNGGFGQRADSLAGVIPENLVASWYWTDQFVTEIIFHNRIMKHKC 180

Query: 181 RVKEPESATAFYVPFYAGLAVGKFLWTASSAEDRDHHCRMILRWLSDQEYYKKSNGWDHF 240
           RVKEPESATAFYVPFYAGLAVGKFLWTASSAEDRDHHCRMIL+WLSDQEYYKKSNGWDHF
Sbjct: 181 RVKEPESATAFYVPFYAGLAVGKFLWTASSAEDRDHHCRMILKWLSDQEYYKKSNGWDHF 240

Query: 241 ITMGRITWDFRRSSDEDWGSRCIYLPGMRNITRLLIERNPWDYFDVGVPYPTGFHPSSQD 300
           ITMGRITWDFRRSSDEDWGSRCIYLPGMRNITRLLIERNPWDYFDVGVPYPTGFHPSSQD
Sbjct: 241 ITMGRITWDFRRSSDEDWGSRCIYLPGMRNITRLLIERNPWDYFDVGVPYPTGFHPSSQD 300

Query: 301 DMSAWQDFVRTRSRTHLFCFAGAKRAAFRNDFRGILLDQCRNSTAEKCRVVDCAGSRCSN 360
           DMSAWQDFVRT SRTHLFCFAGAKRAAFRNDFRGILLDQCRNSTAEKCRVVDCAGSRCSN
Sbjct: 301 DMSAWQDFVRTHSRTHLFCFAGAKRAAFRNDFRGILLDQCRNSTAEKCRVVDCAGSRCSN 360

Query: 361 GTSAILESFLNSDFCLQPRGDSFTRRSIFDCMVAGAIPVFFWRRTAYYQYEWFLPGKPES 420
           GTSAILESFLNSDFCLQPRGDSFTRRSIFDCMVAGAIPVFFWRRTAYYQYEWFLPGKPES
Sbjct: 361 GTSAILESFLNSDFCLQPRGDSFTRRSIFDCMVAGAIPVFFWRRTAYYQYEWFLPGKPES 420

Query: 421 YSVFIDRNAVKNGTASIEAVLEKFSREEVRAMRDRVIESIPKFVYGTGAVRDAFDVAVDG 480
           YSVFIDRNAVKNGTASIEAVLEKFSREEVR MR+RVIESIP FVYGTGAV+DAFDVAVDG
Sbjct: 421 YSVFIDRNAVKNGTASIEAVLEKFSREEVREMRERVIESIPMFVYGTGAVKDAFDVAVDG 480

Query: 481 VLRRFKEQEEWGYKWK 497
           VLRRFKEQEEWGYKWK
Sbjct: 481 VLRRFKEQEEWGYKWK 494

BLAST of Carg08460 vs. ExPASy Swiss-Prot
Match: F4K6F1 (Xyloglucan galactosyltransferase XLT2 OS=Arabidopsis thaliana OX=3702 GN=XLT2 PE=1 SV=1)

HSP 1 Score: 649.4 bits (1674), Expect = 3.2e-185
Identity = 317/521 (60.84%), Postives = 387/521 (74.28%), Query Frame = 0

Query: 1   MLPLSGNSSPEHHPRKLK---SYELFGRKNSFNSL----LQQFHHQQSR---LWLLLTIL 60
           MLP+S  SSPEH  +K +   S     RKNSFNSL     +  +   SR    WL+L++L
Sbjct: 1   MLPVSNPSSPEHLLKKSRTPDSTTSIDRKNSFNSLHSVGNRSSYIAASRSHCTWLILSLL 60

Query: 61  CLQILLLFIIRFLSLPLP-------LPLPPAISSAANRLHRFPSAAVSP---ASTDGDHC 120
            LQ++L   +R  S+P P        P P A+ +         +A+ +P   +S+  + C
Sbjct: 61  SLQLILFLTLR--SIPFPHRHIPENFPSPAAVVTTTVTTTVISAASSNPPLSSSSSDERC 120

Query: 121 KNGRIFVYDLPTAFNEDIVENCDSLNPWSSSCSAMVNGGFGQRADSLAGVIPENLLASWY 180
            +GR+FVYD+P  FNE I++ CD+LNPWSS C A+ N GFGQ A SL+ VIP++L+ SW+
Sbjct: 121 DSGRVFVYDMPKIFNEVILQQCDNLNPWSSRCDALSNDGFGQEATSLSNVIPKDLVQSWF 180

Query: 181 WTDQFVTEIIFHNRIMKHKCRVKEPESATAFYVPFYAGLAVGKFLWTASSAEDRDHHCRM 240
           WTDQFVTEIIFHNRI+ H+CR  +PESATAFY+PFYAGLAVG++LW+  +A DRD HC+M
Sbjct: 181 WTDQFVTEIIFHNRILNHRCRTLDPESATAFYIPFYAGLAVGQYLWSNYAAADRDRHCKM 240

Query: 241 ILRWLSDQEYYKKSNGWDHFITMGRITWDFRRSSDEDWGSRCIYLPGMRNITRLLIERNP 300
           + +W+ +Q Y+ +SNGWDHFITMGRITWDFRRS DEDWGS CIY+PGMRNITRLLIERN 
Sbjct: 241 MTQWVKNQPYWNRSNGWDHFITMGRITWDFRRSKDEDWGSNCIYIPGMRNITRLLIERNS 300

Query: 301 WDYFDVGVPYPTGFHPSSQDDMSAWQDFVRTRSRTHLFCFAGAKRAAFRNDFRGILLDQC 360
           WD+FDVGVPYPTGFHP S  D+  WQDFVR R R  LFCFAGA RA   NDFRG+LL  C
Sbjct: 301 WDHFDVGVPYPTGFHPRSDSDVVNWQDFVRNRRRETLFCFAGAPRAGIVNDFRGLLLRHC 360

Query: 361 RNSTAEKCRVVDCAGSRCSNGTSAILESFLNSDFCLQPRGDSFTRRSIFDCMVAGAIPVF 420
             S   KCR VDC   +CSNG+SAILE+FL SDFCLQPRGDSFTRRSIFDCM+AG+IPVF
Sbjct: 361 EESRG-KCRTVDCTVGKCSNGSSAILETFLGSDFCLQPRGDSFTRRSIFDCMLAGSIPVF 420

Query: 421 FWRRTAYYQYEWFLPGKPESYSVFIDRNAVKNGTASIEAVLEKFSREEVRAMRDRVIESI 480
           FWRR+AY QY+WFLP KP+SYSVFIDRN V NGT SI+ VLE++S+E+VR MR+RVI+ I
Sbjct: 421 FWRRSAYMQYQWFLPDKPDSYSVFIDRNEVTNGTTSIKEVLERYSKEDVRKMRERVIDLI 480

Query: 481 PKFVY-----GTGAVRDAFDVAVDGVLRRFKEQEEWGYKWK 497
           P  VY     G    +DAFDVA+DGV RRFKEQE+W YKW+
Sbjct: 481 PNLVYAKSPNGLETFKDAFDVAIDGVFRRFKEQEKW-YKWR 517

BLAST of Carg08460 vs. ExPASy Swiss-Prot
Match: O81072 (Probable xyloglucan galactosyltransferase GT11 OS=Arabidopsis thaliana OX=3702 GN=GT11 PE=2 SV=1)

HSP 1 Score: 344.4 bits (882), Expect = 2.2e-93
Identity = 181/448 (40.40%), Postives = 260/448 (58.04%), Query Frame = 0

Query: 75  ISSAANRLHRFPSAAVSPAST-DGDHCKNGRIFVYDLPTAFNEDIVENCDSLNPWSSSCS 134
           + S  N+  R     + P  T   D CK   ++++++P  FNE++++NC +L+ W+  C 
Sbjct: 263 VISKRNQPSRVGKVMLRPRETRSNDPCKGKYVYMHEVPALFNEELLKNCWTLSRWTDMCE 322

Query: 135 AMVNGGFGQRADSLAGVIPENLLASWYWTDQFVTEIIFHNRIMKHKCRVKEPESATAFYV 194
              N G G R  ++ GV      + WY T+QF  E+IFHNR+ ++KC  K+   A+A YV
Sbjct: 323 LTSNFGLGPRLPNMEGV------SGWYATNQFTLEVIFHNRMKQYKCLTKDSSLASAVYV 382

Query: 195 PFYAGLAVGKFLWTASSAEDRDHHCRMILRWLSDQEYYKKSNGWDHFITMGRITWDFRRS 254
           P+Y GL + +FLW       RD     +++WL + + +K+ +G DHF+  GR TWDF R+
Sbjct: 383 PYYPGLDLMRFLW-GPFPFMRDAAALDLMKWLRESQEWKRMDGRDHFMVAGRTTWDFMRT 442

Query: 255 --SDEDWGSRCIYLPGMRNITRLLIERNPWDYFDVGVPYPTGFHPSSQDDMSAWQDFVRT 314
             ++ DWG+R + LP +RN+T LLIE +PW+Y    VPYPT FHPS+  ++  WQ  +R 
Sbjct: 443 PENESDWGNRLMILPEVRNMTMLLIESSPWNYHGFAVPYPTYFHPSTYAEIIQWQMRMRR 502

Query: 315 RSRTHLFCFAGAKRAAFRNDFRGILLDQCRNSTAEKCRVVDC-AGSRCSNGTSAILESFL 374
            +R +LF F GA R    +  R  ++DQC+ ++  KC++++C +GS+       I++ FL
Sbjct: 503 INRRYLFSFVGAPRPNLGDSIRTEIMDQCK-ASKRKCKLLECISGSQKCYKPDQIMKFFL 562

Query: 375 NSDFCLQPRGDSFTRRSIFDCMVAGAIPVFFWRRTAYYQYEWFLPGKPESYSVFIDRNAV 434
           +S FCLQP GDS+TRRS FD ++AG IPVFF   +AY QY W LP     YSVFI    V
Sbjct: 563 SSTFCLQPPGDSYTRRSTFDSILAGCIPVFFHPGSAYAQYIWHLPKDIAKYSVFIPEKNV 622

Query: 435 KNGTASIEAVLEKFSREEVRAMRDRVIESIPKFVY--------GTGAVRDAFDVAVDGVL 494
           K G  SIE VL +  R +V AMR++VI  IP+ +Y         TG   DAFDVAV+GVL
Sbjct: 623 KEGKVSIENVLSRIPRTKVFAMREQVIRLIPRLMYFHPSSKSEDTGRFEDAFDVAVEGVL 682

BLAST of Carg08460 vs. ExPASy Swiss-Prot
Match: Q8H038 (Xyloglucan galactosyltransferase KATAMARI1 homolog OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0144800 PE=2 SV=1)

HSP 1 Score: 329.7 bits (844), Expect = 5.6e-89
Identity = 168/422 (39.81%), Postives = 241/422 (57.11%), Query Frame = 0

Query: 70  PLPPAISSAANRLHRFPSAAVSPASTDGDHCKNGRIFVYDLPTAFNEDIVENCDSLNPWS 129
           P   A   AA R + F   A+  A    D C    I+V++LP  FN+D++  C+ L+ W+
Sbjct: 99  PTAVAHQEAAPRDYAF-QRALKTAENKSDPCGGRYIYVHELPPRFNDDMLRECERLSLWT 158

Query: 130 SSCSAMVNGGFGQRADSLAGVIPENLLASWYWTDQFVTEIIFHNRIMKHKCRVKEPESAT 189
           + C  M N G G    +  GV        WY T+QF+ ++IF NR+ +++C  K+   A 
Sbjct: 159 NMCKFMSNEGLGPPLGNEEGVFSN---TGWYATNQFMVDVIFRNRMKQYECLTKDSSIAA 218

Query: 190 AFYVPFYAGLAVGKFLWTASSAEDRDHHCRMILRWLSDQEYYKKSNGWDHFITMGRITWD 249
           A +VPFYAG  V ++LW   +   RD     ++ WL  +  +    G DHF+  GRI WD
Sbjct: 219 AVFVPFYAGFDVARYLW-GHNISTRDAASLDLIDWLRKRPEWNVMGGRDHFLVGGRIAWD 278

Query: 250 FRRSSDE--DWGSRCIYLPGMRNITRLLIERNPWDYFDVGVPYPTGFHPSSQDDMSAWQD 309
           FRR +DE  DWG++ +++P  +N++ L++E +PW+  D  +PYPT FHP+   D+  WQD
Sbjct: 279 FRRLTDEESDWGNKLLFMPAAKNMSMLVVESSPWNANDFAIPYPTYFHPAKDADVLLWQD 338

Query: 310 FVRTRSRTHLFCFAGAKRAAFRNDFRGILLDQCRNSTAEKCRVVDCAGSRCSNGTSAILE 369
            +R+  R  LF FAGA R       R  L+DQCR S+  K    D   S+C +  SAI+ 
Sbjct: 339 RMRSLERPWLFSFAGAPRPDDPKSIRSQLIDQCRTSSVCKLLECDLGESKC-HSPSAIMN 398

Query: 370 SFLNSDFCLQPRGDSFTRRSIFDCMVAGAIPVFFWRRTAYYQYEWFLPGKPESYSVFIDR 429
            F NS FCLQP+GDS+TRRS FD M+AG IPVFF   +AY QY W LP     YSVFI  
Sbjct: 399 MFQNSLFCLQPQGDSYTRRSAFDSMLAGCIPVFFHPGSAYVQYTWHLPKNYTRYSVFIPE 458

Query: 430 NAVKNGTASIEAVLEKFSREEVRAMRDRVIESIPKFVYG-----TGAVRDAFDVAVDGVL 485
           + V+ G  SIE  L+    + V+ MR+ VI  IP+ +Y         ++DAFDV+V+ ++
Sbjct: 459 DGVRKGNVSIEDRLKSIHPDMVKKMREEVISLIPRVIYADPRSKLETLKDAFDVSVEAII 514

BLAST of Carg08460 vs. ExPASy Swiss-Prot
Match: Q7XJ98 (Xyloglucan galactosyltransferase MUR3 OS=Arabidopsis thaliana OX=3702 GN=MUR3 PE=1 SV=1)

HSP 1 Score: 329.3 bits (843), Expect = 7.3e-89
Identity = 164/418 (39.23%), Postives = 240/418 (57.42%), Query Frame = 0

Query: 72  PPAISSAANRLHRFPSAAVSPASTDGDHCKNGRIFVYDLPTAFNEDIVENCDSLNPWSSS 131
           PP I  +  +       A+       D C    I+V++LP+ FNED++ +C  L+ W++ 
Sbjct: 123 PPRIVESGKKQEFSFIRALKTVDNKSDPCGGKYIYVHNLPSKFNEDMLRDCKKLSLWTNM 182

Query: 132 CSAMVNGGFGQRADSLAGVIPENLLASWYWTDQFVTEIIFHNRIMKHKCRVKEPESATAF 191
           C    N G G   +++ GV  +     WY T+QF  ++IF NR+ ++KC   +   A A 
Sbjct: 183 CKFTTNAGLGPPLENVEGVFSDE---GWYATNQFAVDVIFSNRMKQYKCLTNDSSLAAAI 242

Query: 192 YVPFYAGLAVGKFLWTASSAEDRDHHCRMILRWLSDQEYYKKSNGWDHFITMGRITWDFR 251
           +VPFYAG  + ++LW   +   RD     ++ WL  +  +    G DHF+  GRITWDFR
Sbjct: 243 FVPFYAGFDIARYLW-GYNISRRDAASLELVDWLMKRPEWDIMRGKDHFLVAGRITWDFR 302

Query: 252 RSSDE--DWGSRCIYLPGMRNITRLLIERNPWDYFDVGVPYPTGFHPSSQDDMSAWQDFV 311
           R S+E  DWG++ ++LP  +N++ L++E +PW+  D G+PYPT FHP+   ++  WQD +
Sbjct: 303 RLSEEETDWGNKLLFLPAAKNMSMLVVESSPWNANDFGIPYPTYFHPAKDSEVFEWQDRM 362

Query: 312 RTRSRTHLFCFAGAKRAAFRNDFRGILLDQCRNSTAEKCRVVDCAGSRCSNGTSAILESF 371
           R   R  LF FAGA R       RG ++DQCRNS   K    D   S+C +  S+I++ F
Sbjct: 363 RNLERKWLFSFAGAPRPDNPKSIRGQIIDQCRNSNVGKLLECDFGESKC-HAPSSIMQMF 422

Query: 372 LNSDFCLQPRGDSFTRRSIFDCMVAGAIPVFFWRRTAYYQYEWFLPGKPESYSVFIDRNA 431
            +S FCLQP+GDS+TRRS FD M+AG IPVFF   +AY QY W LP    +YSVFI  + 
Sbjct: 423 QSSLFCLQPQGDSYTRRSAFDSMLAGCIPVFFHPGSAYTQYTWHLPKNYTTYSVFIPEDD 482

Query: 432 VKNGTASIEAVLEKFSREEVRAMRDRVIESIPKFVYG-----TGAVRDAFDVAVDGVL 483
           V+    SIE  L +   ++V+ MR+ VI  IP+ +Y          +DAFDV+V  V+
Sbjct: 483 VRKRNISIEERLLQIPAKQVKIMRENVINLIPRLIYADPRSELETQKDAFDVSVQAVI 535

BLAST of Carg08460 vs. ExPASy Swiss-Prot
Match: Q84R16 (Probable xyloglucan galactosyltransferase GT14 OS=Arabidopsis thaliana OX=3702 GN=GT14 PE=2 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 4.2e-84
Identity = 168/418 (40.19%), Postives = 246/418 (58.85%), Query Frame = 0

Query: 84  RFPSAAVSPASTDGDHCKNGRIFVYDLPTAFNEDIVENCDSLNPWSSS--CSAMVNGGFG 143
           RFP    SP S+    C    I+V++LP  FN D+++NC  +   +    C  + N GFG
Sbjct: 84  RFPDDP-SPDSS----CSGRYIYVHELPYRFNGDLLDNCFKITRGTEKDICPYIENYGFG 143

Query: 144 QRADSLAGVIPENLLASWYWTDQFVTEIIFHNRIMKHKCRVKEPESATAFYVPFYAGLAV 203
               +   V+   L  SW+ T+QF+ E+IFHN+++ ++C   +   A+A +VPFYAGL +
Sbjct: 144 PVIKNYENVL---LKQSWFTTNQFMLEVIFHNKMINYRCLTNDSSLASAVFVPFYAGLDM 203

Query: 204 GKFLWTASSAEDRDHHCRMILRWLSDQEYYKKSNGWDHFITMGRITWDFRRSSD--EDWG 263
            ++LW   +   RD     ++ WL  Q+ + + +G DHF+  GRI WDFRR +D   DWG
Sbjct: 204 SRYLW-GFNITVRDSSSHELMDWLVVQKEWGRMSGRDHFLVSGRIAWDFRRQTDNESDWG 263

Query: 264 SRCIYLPGMRNITRLLIERNPWDYFDVGVPYPTGFHPSSQDDMSAWQDFVRTRSRTHLFC 323
           S+  +LP  RN++ L IE + W   D  +PYPT FHP S D++  WQ+ +R+R R +LF 
Sbjct: 264 SKLRFLPESRNMSMLSIESSSWKN-DYAIPYPTCFHPRSVDEIVEWQELMRSRKREYLFT 323

Query: 324 FAGAKRAAFRNDFRGILLDQCRNSTAEKCRVVDC--AGSRCSNGTSAILESFLNSDFCLQ 383
           FAGA R  +++  RG ++D+C  S  ++C ++DC      C N  + +++ F NS FCLQ
Sbjct: 324 FAGAPRPEYKDSVRGKIIDECLES-KKQCYLLDCNYGNVNCDNPVN-VMKVFRNSVFCLQ 383

Query: 384 PRGDSFTRRSIFDCMVAGAIPVFFWRRTAYYQYEWFLPGKPESYSVFIDRNAVKNGTASI 443
           P GDS+TRRS+FD ++AG IPVFF   TAY QY+W LP    SYSV++    VK     I
Sbjct: 384 PPGDSYTRRSMFDSILAGCIPVFFHPGTAYAQYKWHLPKNHSSYSVYLPVKDVKEWNIKI 443

Query: 444 EAVLEKFSREEVRAMRDRVIESIPKFV-----YGTGAVRDAFDVAVDGVLRRFKEQEE 491
           +  L +   E V  +R+ VI  IPK V     YG+    DAF++AV G+L R +E  E
Sbjct: 444 KERLIEIPEERVVRLREEVIRLIPKVVYADPKYGSDGSEDAFELAVKGMLERIEEVRE 489

BLAST of Carg08460 vs. ExPASy TrEMBL
Match: A0A6J1GZT1 (xyloglucan galactosyltransferase XLT2-like OS=Cucurbita moschata OX=3662 GN=LOC111459117 PE=3 SV=1)

HSP 1 Score: 1020.4 bits (2637), Expect = 2.6e-294
Identity = 487/496 (98.19%), Postives = 490/496 (98.79%), Query Frame = 0

Query: 1   MLPLSGNSSPEHHPRKLKSYELFGRKNSFNSLLQQFHHQQSRLWLLLTILCLQILLLFII 60
           MLPLSGNSSPEHHPRKLKSYELFGRKNSFNSLLQQFHHQQS LWLLLTILCLQILLLFII
Sbjct: 1   MLPLSGNSSPEHHPRKLKSYELFGRKNSFNSLLQQFHHQQSCLWLLLTILCLQILLLFII 60

Query: 61  RFLSLPLPLPLPPAISSAANRLHRFPSAAVSPASTDGDHCKNGRIFVYDLPTAFNEDIVE 120
           RFLSLPLPLPLPPAISSAANRLHRFPSAAVSPASTDGDHCKNGRIFVYDLPT FN+DIV+
Sbjct: 61  RFLSLPLPLPLPPAISSAANRLHRFPSAAVSPASTDGDHCKNGRIFVYDLPTEFNQDIVQ 120

Query: 121 NCDSLNPWSSSCSAMVNGGFGQRADSLAGVIPENLLASWYWTDQFVTEIIFHNRIMKHKC 180
           NCDSLNPWSSSCSAMVNGGFGQRADSLAGVIPENLLASWYWTDQFVTEIIFHNRIMKHKC
Sbjct: 121 NCDSLNPWSSSCSAMVNGGFGQRADSLAGVIPENLLASWYWTDQFVTEIIFHNRIMKHKC 180

Query: 181 RVKEPESATAFYVPFYAGLAVGKFLWTASSAEDRDHHCRMILRWLSDQEYYKKSNGWDHF 240
           RVKEPESATAFYVPFYAGLAVGKFLWTASSAE RDHHCRMILRWLSDQEYYKKSNGWDHF
Sbjct: 181 RVKEPESATAFYVPFYAGLAVGKFLWTASSAEARDHHCRMILRWLSDQEYYKKSNGWDHF 240

Query: 241 ITMGRITWDFRRSSDEDWGSRCIYLPGMRNITRLLIERNPWDYFDVGVPYPTGFHPSSQD 300
           ITMGRITWDFRRSSDEDWGSRCIYLPGMRNITRLLIERNPWDYFDVGVPYPTGFHPSSQD
Sbjct: 241 ITMGRITWDFRRSSDEDWGSRCIYLPGMRNITRLLIERNPWDYFDVGVPYPTGFHPSSQD 300

Query: 301 DMSAWQDFVRTRSRTHLFCFAGAKRAAFRNDFRGILLDQCRNSTAEKCRVVDCAGSRCSN 360
           DMSAWQDFVRTRSRTHLFCFAGAKRAAFRNDFRGILLDQCRNSTAEKCRVVDCAGSRCSN
Sbjct: 301 DMSAWQDFVRTRSRTHLFCFAGAKRAAFRNDFRGILLDQCRNSTAEKCRVVDCAGSRCSN 360

Query: 361 GTSAILESFLNSDFCLQPRGDSFTRRSIFDCMVAGAIPVFFWRRTAYYQYEWFLPGKPES 420
           GTSAILESFLNSDFCLQPRGDSFTRRSIFDCMVAGAIPVFFWRRTAYYQYEWFLPGKPES
Sbjct: 361 GTSAILESFLNSDFCLQPRGDSFTRRSIFDCMVAGAIPVFFWRRTAYYQYEWFLPGKPES 420

Query: 421 YSVFIDRNAVKNGTASIEAVLEKFSREEVRAMRDRVIESIPKFVYGTGAVRDAFDVAVDG 480
           YSVFIDRNAVKNGT SIEAVLEKFSREEVR MR+RVIESIPKFVYGTGAVRDAFDVAVD 
Sbjct: 421 YSVFIDRNAVKNGTTSIEAVLEKFSREEVREMRERVIESIPKFVYGTGAVRDAFDVAVDR 480

Query: 481 VLRRFKEQEEWGYKWK 497
           VLRRFKEQEEWGYKWK
Sbjct: 481 VLRRFKEQEEWGYKWK 496

BLAST of Carg08460 vs. ExPASy TrEMBL
Match: A0A6J1KAD2 (xyloglucan galactosyltransferase XLT2-like OS=Cucurbita maxima OX=3661 GN=LOC111491557 PE=3 SV=1)

HSP 1 Score: 1006.5 bits (2601), Expect = 3.8e-290
Identity = 480/496 (96.77%), Postives = 488/496 (98.39%), Query Frame = 0

Query: 1   MLPLSGNSSPEHHPRKLKSYELFGRKNSFNSLLQQFHHQQSRLWLLLTILCLQILLLFII 60
           MLPLSGNSSPEHHPRKLKSYELFGRKNSFNSLLQQFHHQQSRLWLLLTILCLQILL+FII
Sbjct: 1   MLPLSGNSSPEHHPRKLKSYELFGRKNSFNSLLQQFHHQQSRLWLLLTILCLQILLIFII 60

Query: 61  RFLSLPLPLPLPPAISSAANRLHRFPSAAVSPASTDGDHCKNGRIFVYDLPTAFNEDIVE 120
           RFLS   PLPLPP ISSAANRL+RFPSAAVSPASTD DHCKNGRIFVYDLPTAFN+DIV+
Sbjct: 61  RFLS--FPLPLPPVISSAANRLYRFPSAAVSPASTDVDHCKNGRIFVYDLPTAFNQDIVK 120

Query: 121 NCDSLNPWSSSCSAMVNGGFGQRADSLAGVIPENLLASWYWTDQFVTEIIFHNRIMKHKC 180
           NCDSLNPWSSSCSAMVNGGFGQRADSLAGVIPENL+ASWYWTDQFVTEIIFHNRIMKHKC
Sbjct: 121 NCDSLNPWSSSCSAMVNGGFGQRADSLAGVIPENLVASWYWTDQFVTEIIFHNRIMKHKC 180

Query: 181 RVKEPESATAFYVPFYAGLAVGKFLWTASSAEDRDHHCRMILRWLSDQEYYKKSNGWDHF 240
           RVKEPESATAFYVPFYAGLAVGKFLWTASSAEDRDHHCRMIL+WLSDQEYYKKSNGWDHF
Sbjct: 181 RVKEPESATAFYVPFYAGLAVGKFLWTASSAEDRDHHCRMILKWLSDQEYYKKSNGWDHF 240

Query: 241 ITMGRITWDFRRSSDEDWGSRCIYLPGMRNITRLLIERNPWDYFDVGVPYPTGFHPSSQD 300
           ITMGRITWDFRRSSDEDWGSRCIYLPGMRNITRLLIERNPWDYFDVGVPYPTGFHPSSQD
Sbjct: 241 ITMGRITWDFRRSSDEDWGSRCIYLPGMRNITRLLIERNPWDYFDVGVPYPTGFHPSSQD 300

Query: 301 DMSAWQDFVRTRSRTHLFCFAGAKRAAFRNDFRGILLDQCRNSTAEKCRVVDCAGSRCSN 360
           DMSAWQDFVRT SRTHLFCFAGAKRAAFRNDFRGILLDQCRNSTAEKCRVVDCAGSRCSN
Sbjct: 301 DMSAWQDFVRTHSRTHLFCFAGAKRAAFRNDFRGILLDQCRNSTAEKCRVVDCAGSRCSN 360

Query: 361 GTSAILESFLNSDFCLQPRGDSFTRRSIFDCMVAGAIPVFFWRRTAYYQYEWFLPGKPES 420
           GTSAILESFLNSDFCLQPRGDSFTRRSIFDCMVAGAIPVFFWRRTAYYQYEWFLPGKPES
Sbjct: 361 GTSAILESFLNSDFCLQPRGDSFTRRSIFDCMVAGAIPVFFWRRTAYYQYEWFLPGKPES 420

Query: 421 YSVFIDRNAVKNGTASIEAVLEKFSREEVRAMRDRVIESIPKFVYGTGAVRDAFDVAVDG 480
           YSVFIDRNAVKNGTASIEAVLEKFSREEVR MR+RVIESIP FVYGTGAV+DAFDVAVDG
Sbjct: 421 YSVFIDRNAVKNGTASIEAVLEKFSREEVREMRERVIESIPMFVYGTGAVKDAFDVAVDG 480

Query: 481 VLRRFKEQEEWGYKWK 497
           VLRRFKEQEEWGYKWK
Sbjct: 481 VLRRFKEQEEWGYKWK 494

BLAST of Carg08460 vs. ExPASy TrEMBL
Match: A0A6J1I261 (xyloglucan galactosyltransferase XLT2-like OS=Cucurbita maxima OX=3661 GN=LOC111469155 PE=3 SV=1)

HSP 1 Score: 905.2 bits (2338), Expect = 1.2e-259
Identity = 427/498 (85.74%), Postives = 456/498 (91.57%), Query Frame = 0

Query: 1   MLPLSGNSSP-EHHPRKLKSYELFGRKNSFNSLLQQFHHQQSRLWLLLTILCLQILLLFI 60
           MLPLSGNSSP EHHP+K KS ELF +KN+FNSLL QFHHQQSRLWLLL ILCLQILLLF 
Sbjct: 1   MLPLSGNSSPAEHHPKKFKSSELFDKKNAFNSLLHQFHHQQSRLWLLLVILCLQILLLFT 60

Query: 61  IRFLSLPLPLPLPPAISSAANRLHRFPS-AAVSPASTDGDHCKNGRIFVYDLPTAFNEDI 120
           IRF    LPLPLPPA+SS+ +RL RFPS A VS  + D D C+NGRIFVYDLPTAFN+DI
Sbjct: 61  IRF----LPLPLPPALSSSTDRLRRFPSVAVVSSTANDADECRNGRIFVYDLPTAFNKDI 120

Query: 121 VENCDSLNPWSSSCSAMVNGGFGQRADSLAGVIPENLLASWYWTDQFVTEIIFHNRIMKH 180
           +ENCD+LNPWSSSC AM NGGFGQRADSLAG+IPENLL SWYWTDQFVTEIIFHNRIMKH
Sbjct: 121 LENCDNLNPWSSSCRAMANGGFGQRADSLAGIIPENLLPSWYWTDQFVTEIIFHNRIMKH 180

Query: 181 KCRVKEPESATAFYVPFYAGLAVGKFLWTASSAEDRDHHCRMILRWLSDQEYYKKSNGWD 240
           KCRV EP  ATAFY+PFYAGLAVGKFLWT S+ EDRD HCRMIL+WLSDQE+YKKSNGWD
Sbjct: 181 KCRVSEPGLATAFYIPFYAGLAVGKFLWTNSTVEDRDQHCRMILKWLSDQEHYKKSNGWD 240

Query: 241 HFITMGRITWDFRRSSDEDWGSRCIYLPGMRNITRLLIERNPWDYFDVGVPYPTGFHPSS 300
           HFITMGRITWDFRRS D+DWGS CIYLPGMRNITRLLIERNPWDYFDVGVPYPTGFHP  
Sbjct: 241 HFITMGRITWDFRRSKDKDWGSECIYLPGMRNITRLLIERNPWDYFDVGVPYPTGFHPRG 300

Query: 301 QDDMSAWQDFVRTRSRTHLFCFAGAKRAAFRNDFRGILLDQCRNSTAEKCRVVDCAGSRC 360
           Q+D++AWQDFVRTR RTHLFCFAGA RAAF NDFR +LLDQCRNSTAEKCRVVDCAGSRC
Sbjct: 301 QNDVTAWQDFVRTRRRTHLFCFAGATRAAFGNDFRAVLLDQCRNSTAEKCRVVDCAGSRC 360

Query: 361 SNGTSAILESFLNSDFCLQPRGDSFTRRSIFDCMVAGAIPVFFWRRTAYYQYEWFLPGKP 420
           +NGT+AILE+FL+SDFCLQPRGDSFTRRSIFDCM+AGAIPVFFWRRTAYYQYEWFLP +P
Sbjct: 361 TNGTTAILETFLSSDFCLQPRGDSFTRRSIFDCMIAGAIPVFFWRRTAYYQYEWFLPSEP 420

Query: 421 ESYSVFIDRNAVKNGTASIEAVLEKFSREEVRAMRDRVIESIPKFVYGTGAVRDAFDVAV 480
           ESYSVFIDRNAVKNGTASIEAVLE+FSREEVR MR+RVIESIPK VYGTG VRDAFDVAV
Sbjct: 421 ESYSVFIDRNAVKNGTASIEAVLERFSREEVREMRERVIESIPKLVYGTGGVRDAFDVAV 480

Query: 481 DGVLRRFKEQEEWGYKWK 497
           DGVLRRFKEQ EWGYKWK
Sbjct: 481 DGVLRRFKEQNEWGYKWK 494

BLAST of Carg08460 vs. ExPASy TrEMBL
Match: A0A6J1EL91 (xyloglucan galactosyltransferase XLT2-like OS=Cucurbita moschata OX=3662 GN=LOC111435673 PE=3 SV=1)

HSP 1 Score: 904.8 bits (2337), Expect = 1.6e-259
Identity = 429/497 (86.32%), Postives = 455/497 (91.55%), Query Frame = 0

Query: 1   MLPLSGNSSP-EHHPRKLKSYELFGRKNSFNSLLQQFHHQQSRLWLLLTILCLQILLLFI 60
           MLPLSGNSSP EHHP+K KS ELF +KN+FNSLLQQFHHQQSRLWLLL IL LQILLLF 
Sbjct: 1   MLPLSGNSSPAEHHPKKFKSSELFDKKNAFNSLLQQFHHQQSRLWLLLVILSLQILLLFT 60

Query: 61  IRFLSLPLPLPLPPAISSAANRLHRFPSAAVSPASTDGDHCKNGRIFVYDLPTAFNEDIV 120
           IRF    LPLPLPPA+SS+ NRL RFPS AV  +S   D CKNGRIFVYDLP+AFN+DI+
Sbjct: 61  IRF----LPLPLPPALSSSTNRLRRFPSVAV-VSSAAADECKNGRIFVYDLPSAFNKDIL 120

Query: 121 ENCDSLNPWSSSCSAMVNGGFGQRADSLAGVIPENLLASWYWTDQFVTEIIFHNRIMKHK 180
           ENCD+LNPWSSSC AM NGGFGQRADSLAG+IPENLL SWYWTDQFVTEIIFHNRI+KHK
Sbjct: 121 ENCDNLNPWSSSCRAMANGGFGQRADSLAGIIPENLLPSWYWTDQFVTEIIFHNRIVKHK 180

Query: 181 CRVKEPESATAFYVPFYAGLAVGKFLWTASSAEDRDHHCRMILRWLSDQEYYKKSNGWDH 240
           CRV EPE ATAFY+PFYAGLAVGKFLWT S+ EDRD HCRMILRWL+DQEYYKKSNGWDH
Sbjct: 181 CRVSEPELATAFYIPFYAGLAVGKFLWTNSTVEDRDQHCRMILRWLADQEYYKKSNGWDH 240

Query: 241 FITMGRITWDFRRSSDEDWGSRCIYLPGMRNITRLLIERNPWDYFDVGVPYPTGFHPSSQ 300
           FITMGRITWDFRRS D+DWGS CIYLPGMRNITRLLIERNPWDYFDVGVPYPTGFHP  Q
Sbjct: 241 FITMGRITWDFRRSKDKDWGSECIYLPGMRNITRLLIERNPWDYFDVGVPYPTGFHPRGQ 300

Query: 301 DDMSAWQDFVRTRSRTHLFCFAGAKRAAFRNDFRGILLDQCRNSTAEKCRVVDCAGSRCS 360
           DD++AWQDFVRTR RTHLFCFAGA RAAF NDFR +LLDQCRNSTAEKCRVVDCAGSRC+
Sbjct: 301 DDVTAWQDFVRTRRRTHLFCFAGATRAAFGNDFRAVLLDQCRNSTAEKCRVVDCAGSRCT 360

Query: 361 NGTSAILESFLNSDFCLQPRGDSFTRRSIFDCMVAGAIPVFFWRRTAYYQYEWFLPGKPE 420
           NGTSAILE+FL+SDFCLQPRGDSFTRRSIFDCM+AGAIPVFFWRRTAYYQYEWFLP +P 
Sbjct: 361 NGTSAILETFLSSDFCLQPRGDSFTRRSIFDCMIAGAIPVFFWRRTAYYQYEWFLPSEPG 420

Query: 421 SYSVFIDRNAVKNGTASIEAVLEKFSREEVRAMRDRVIESIPKFVYGTGAVRDAFDVAVD 480
           SYSVFIDRNAVKNGTASIEAVLE+FSREEVR MR+RVIESIPK VYGTG VRDAFDVAVD
Sbjct: 421 SYSVFIDRNAVKNGTASIEAVLERFSREEVREMRERVIESIPKLVYGTGGVRDAFDVAVD 480

Query: 481 GVLRRFKEQEEWGYKWK 497
           GVLRRFKEQ EWGYKWK
Sbjct: 481 GVLRRFKEQNEWGYKWK 492

BLAST of Carg08460 vs. ExPASy TrEMBL
Match: A0A5D3DAU5 (Xyloglucan galactosyltransferase XLT2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold480G001220 PE=3 SV=1)

HSP 1 Score: 899.4 bits (2323), Expect = 6.6e-258
Identity = 426/499 (85.37%), Postives = 457/499 (91.58%), Query Frame = 0

Query: 1   MLPLSGNSSP-EHHPRKLK-SYELFGRKNSFNSLLQQFHHQQSRLWLLLTILCLQILLLF 60
           MLPLSGNSSP EHHP+K K S E F +KNSFNSLLQQFHHQQSRLWLLL IL LQILLLF
Sbjct: 1   MLPLSGNSSPAEHHPKKFKASSEFFDKKNSFNSLLQQFHHQQSRLWLLLVILFLQILLLF 60

Query: 61  IIRFLSLPLPLPLPPAISSAANR-LHRFPSAAVSPASTDGDHCKNGRIFVYDLPTAFNED 120
            IR+    LPLPLPPA+SS+ N+ LHRFPS AVSPA  DG +CKNGRIFVYDLPT FN+D
Sbjct: 61  TIRY----LPLPLPPALSSSTNKQLHRFPSVAVSPADIDGGNCKNGRIFVYDLPTVFNQD 120

Query: 121 IVENCDSLNPWSSSCSAMVNGGFGQRADSLAGVIPENLLASWYWTDQFVTEIIFHNRIMK 180
           I++NCD+LNPWSSSCSAM NGGFGQRADSLAG+IPENLL SWYWTDQFVTEIIFHNRI+K
Sbjct: 121 ILDNCDNLNPWSSSCSAMANGGFGQRADSLAGIIPENLLQSWYWTDQFVTEIIFHNRILK 180

Query: 181 HKCRVKEPESATAFYVPFYAGLAVGKFLWTASSAEDRDHHCRMILRWLSDQEYYKKSNGW 240
           HKCRV EPESATAFY+PFYAGLAVGKFLWT S+AE+RD HCR IL+WLSDQEYYKKSNGW
Sbjct: 181 HKCRVLEPESATAFYIPFYAGLAVGKFLWTNSTAEERDQHCRSILKWLSDQEYYKKSNGW 240

Query: 241 DHFITMGRITWDFRRSSDEDWGSRCIYLPGMRNITRLLIERNPWDYFDVGVPYPTGFHPS 300
           DHFITMGRITWDFRRS D+DWGS CIYLPGMRNITRLLIERNPWDYFDVGVPYPTGFHP 
Sbjct: 241 DHFITMGRITWDFRRSKDKDWGSGCIYLPGMRNITRLLIERNPWDYFDVGVPYPTGFHPK 300

Query: 301 SQDDMSAWQDFVRTRSRTHLFCFAGAKRAAFRNDFRGILLDQCRNSTAEKCRVVDCAGSR 360
           SQ+D+SAWQ F+RTR RTHLFCFAGA RAAFR DFR +LL QC+NST EKCRVVDCAGSR
Sbjct: 301 SQNDISAWQQFIRTRRRTHLFCFAGATRAAFRKDFRAMLLHQCKNSTGEKCRVVDCAGSR 360

Query: 361 CSNGTSAILESFLNSDFCLQPRGDSFTRRSIFDCMVAGAIPVFFWRRTAYYQYEWFLPGK 420
           CSNGTSAILE+FL SDFCLQPRGDSFTRRSIFDCMVAGAIPVFFWRRTAYYQYEWFLPG+
Sbjct: 361 CSNGTSAILETFLTSDFCLQPRGDSFTRRSIFDCMVAGAIPVFFWRRTAYYQYEWFLPGE 420

Query: 421 PESYSVFIDRNAVKNGTASIEAVLEKFSREEVRAMRDRVIESIPKFVYGTGAVRDAFDVA 480
           P SYSVFIDRNAVKNGT SIEAVLE+FSREEV+ MR+RVIESIPKF+YGTG VRDAFDVA
Sbjct: 421 PGSYSVFIDRNAVKNGTTSIEAVLERFSREEVKEMRERVIESIPKFIYGTGEVRDAFDVA 480

Query: 481 VDGVLRRFKEQEEWGYKWK 497
           ++GVLRRFKEQEEW YKWK
Sbjct: 481 IEGVLRRFKEQEEWEYKWK 495

BLAST of Carg08460 vs. TAIR 10
Match: AT5G62220.1 (glycosyltransferase 18 )

HSP 1 Score: 649.4 bits (1674), Expect = 2.3e-186
Identity = 317/521 (60.84%), Postives = 387/521 (74.28%), Query Frame = 0

Query: 1   MLPLSGNSSPEHHPRKLK---SYELFGRKNSFNSL----LQQFHHQQSR---LWLLLTIL 60
           MLP+S  SSPEH  +K +   S     RKNSFNSL     +  +   SR    WL+L++L
Sbjct: 1   MLPVSNPSSPEHLLKKSRTPDSTTSIDRKNSFNSLHSVGNRSSYIAASRSHCTWLILSLL 60

Query: 61  CLQILLLFIIRFLSLPLP-------LPLPPAISSAANRLHRFPSAAVSP---ASTDGDHC 120
            LQ++L   +R  S+P P        P P A+ +         +A+ +P   +S+  + C
Sbjct: 61  SLQLILFLTLR--SIPFPHRHIPENFPSPAAVVTTTVTTTVISAASSNPPLSSSSSDERC 120

Query: 121 KNGRIFVYDLPTAFNEDIVENCDSLNPWSSSCSAMVNGGFGQRADSLAGVIPENLLASWY 180
            +GR+FVYD+P  FNE I++ CD+LNPWSS C A+ N GFGQ A SL+ VIP++L+ SW+
Sbjct: 121 DSGRVFVYDMPKIFNEVILQQCDNLNPWSSRCDALSNDGFGQEATSLSNVIPKDLVQSWF 180

Query: 181 WTDQFVTEIIFHNRIMKHKCRVKEPESATAFYVPFYAGLAVGKFLWTASSAEDRDHHCRM 240
           WTDQFVTEIIFHNRI+ H+CR  +PESATAFY+PFYAGLAVG++LW+  +A DRD HC+M
Sbjct: 181 WTDQFVTEIIFHNRILNHRCRTLDPESATAFYIPFYAGLAVGQYLWSNYAAADRDRHCKM 240

Query: 241 ILRWLSDQEYYKKSNGWDHFITMGRITWDFRRSSDEDWGSRCIYLPGMRNITRLLIERNP 300
           + +W+ +Q Y+ +SNGWDHFITMGRITWDFRRS DEDWGS CIY+PGMRNITRLLIERN 
Sbjct: 241 MTQWVKNQPYWNRSNGWDHFITMGRITWDFRRSKDEDWGSNCIYIPGMRNITRLLIERNS 300

Query: 301 WDYFDVGVPYPTGFHPSSQDDMSAWQDFVRTRSRTHLFCFAGAKRAAFRNDFRGILLDQC 360
           WD+FDVGVPYPTGFHP S  D+  WQDFVR R R  LFCFAGA RA   NDFRG+LL  C
Sbjct: 301 WDHFDVGVPYPTGFHPRSDSDVVNWQDFVRNRRRETLFCFAGAPRAGIVNDFRGLLLRHC 360

Query: 361 RNSTAEKCRVVDCAGSRCSNGTSAILESFLNSDFCLQPRGDSFTRRSIFDCMVAGAIPVF 420
             S   KCR VDC   +CSNG+SAILE+FL SDFCLQPRGDSFTRRSIFDCM+AG+IPVF
Sbjct: 361 EESRG-KCRTVDCTVGKCSNGSSAILETFLGSDFCLQPRGDSFTRRSIFDCMLAGSIPVF 420

Query: 421 FWRRTAYYQYEWFLPGKPESYSVFIDRNAVKNGTASIEAVLEKFSREEVRAMRDRVIESI 480
           FWRR+AY QY+WFLP KP+SYSVFIDRN V NGT SI+ VLE++S+E+VR MR+RVI+ I
Sbjct: 421 FWRRSAYMQYQWFLPDKPDSYSVFIDRNEVTNGTTSIKEVLERYSKEDVRKMRERVIDLI 480

Query: 481 PKFVY-----GTGAVRDAFDVAVDGVLRRFKEQEEWGYKWK 497
           P  VY     G    +DAFDVA+DGV RRFKEQE+W YKW+
Sbjct: 481 PNLVYAKSPNGLETFKDAFDVAIDGVFRRFKEQEKW-YKWR 517

BLAST of Carg08460 vs. TAIR 10
Match: AT2G29040.1 (Exostosin family protein )

HSP 1 Score: 344.4 bits (882), Expect = 1.6e-94
Identity = 181/448 (40.40%), Postives = 260/448 (58.04%), Query Frame = 0

Query: 75  ISSAANRLHRFPSAAVSPAST-DGDHCKNGRIFVYDLPTAFNEDIVENCDSLNPWSSSCS 134
           + S  N+  R     + P  T   D CK   ++++++P  FNE++++NC +L+ W+  C 
Sbjct: 263 VISKRNQPSRVGKVMLRPRETRSNDPCKGKYVYMHEVPALFNEELLKNCWTLSRWTDMCE 322

Query: 135 AMVNGGFGQRADSLAGVIPENLLASWYWTDQFVTEIIFHNRIMKHKCRVKEPESATAFYV 194
              N G G R  ++ GV      + WY T+QF  E+IFHNR+ ++KC  K+   A+A YV
Sbjct: 323 LTSNFGLGPRLPNMEGV------SGWYATNQFTLEVIFHNRMKQYKCLTKDSSLASAVYV 382

Query: 195 PFYAGLAVGKFLWTASSAEDRDHHCRMILRWLSDQEYYKKSNGWDHFITMGRITWDFRRS 254
           P+Y GL + +FLW       RD     +++WL + + +K+ +G DHF+  GR TWDF R+
Sbjct: 383 PYYPGLDLMRFLW-GPFPFMRDAAALDLMKWLRESQEWKRMDGRDHFMVAGRTTWDFMRT 442

Query: 255 --SDEDWGSRCIYLPGMRNITRLLIERNPWDYFDVGVPYPTGFHPSSQDDMSAWQDFVRT 314
             ++ DWG+R + LP +RN+T LLIE +PW+Y    VPYPT FHPS+  ++  WQ  +R 
Sbjct: 443 PENESDWGNRLMILPEVRNMTMLLIESSPWNYHGFAVPYPTYFHPSTYAEIIQWQMRMRR 502

Query: 315 RSRTHLFCFAGAKRAAFRNDFRGILLDQCRNSTAEKCRVVDC-AGSRCSNGTSAILESFL 374
            +R +LF F GA R    +  R  ++DQC+ ++  KC++++C +GS+       I++ FL
Sbjct: 503 INRRYLFSFVGAPRPNLGDSIRTEIMDQCK-ASKRKCKLLECISGSQKCYKPDQIMKFFL 562

Query: 375 NSDFCLQPRGDSFTRRSIFDCMVAGAIPVFFWRRTAYYQYEWFLPGKPESYSVFIDRNAV 434
           +S FCLQP GDS+TRRS FD ++AG IPVFF   +AY QY W LP     YSVFI    V
Sbjct: 563 SSTFCLQPPGDSYTRRSTFDSILAGCIPVFFHPGSAYAQYIWHLPKDIAKYSVFIPEKNV 622

Query: 435 KNGTASIEAVLEKFSREEVRAMRDRVIESIPKFVY--------GTGAVRDAFDVAVDGVL 494
           K G  SIE VL +  R +V AMR++VI  IP+ +Y         TG   DAFDVAV+GVL
Sbjct: 623 KEGKVSIENVLSRIPRTKVFAMREQVIRLIPRLMYFHPSSKSEDTGRFEDAFDVAVEGVL 682

BLAST of Carg08460 vs. TAIR 10
Match: AT2G20370.1 (Exostosin family protein )

HSP 1 Score: 329.3 bits (843), Expect = 5.2e-90
Identity = 164/418 (39.23%), Postives = 240/418 (57.42%), Query Frame = 0

Query: 72  PPAISSAANRLHRFPSAAVSPASTDGDHCKNGRIFVYDLPTAFNEDIVENCDSLNPWSSS 131
           PP I  +  +       A+       D C    I+V++LP+ FNED++ +C  L+ W++ 
Sbjct: 123 PPRIVESGKKQEFSFIRALKTVDNKSDPCGGKYIYVHNLPSKFNEDMLRDCKKLSLWTNM 182

Query: 132 CSAMVNGGFGQRADSLAGVIPENLLASWYWTDQFVTEIIFHNRIMKHKCRVKEPESATAF 191
           C    N G G   +++ GV  +     WY T+QF  ++IF NR+ ++KC   +   A A 
Sbjct: 183 CKFTTNAGLGPPLENVEGVFSDE---GWYATNQFAVDVIFSNRMKQYKCLTNDSSLAAAI 242

Query: 192 YVPFYAGLAVGKFLWTASSAEDRDHHCRMILRWLSDQEYYKKSNGWDHFITMGRITWDFR 251
           +VPFYAG  + ++LW   +   RD     ++ WL  +  +    G DHF+  GRITWDFR
Sbjct: 243 FVPFYAGFDIARYLW-GYNISRRDAASLELVDWLMKRPEWDIMRGKDHFLVAGRITWDFR 302

Query: 252 RSSDE--DWGSRCIYLPGMRNITRLLIERNPWDYFDVGVPYPTGFHPSSQDDMSAWQDFV 311
           R S+E  DWG++ ++LP  +N++ L++E +PW+  D G+PYPT FHP+   ++  WQD +
Sbjct: 303 RLSEEETDWGNKLLFLPAAKNMSMLVVESSPWNANDFGIPYPTYFHPAKDSEVFEWQDRM 362

Query: 312 RTRSRTHLFCFAGAKRAAFRNDFRGILLDQCRNSTAEKCRVVDCAGSRCSNGTSAILESF 371
           R   R  LF FAGA R       RG ++DQCRNS   K    D   S+C +  S+I++ F
Sbjct: 363 RNLERKWLFSFAGAPRPDNPKSIRGQIIDQCRNSNVGKLLECDFGESKC-HAPSSIMQMF 422

Query: 372 LNSDFCLQPRGDSFTRRSIFDCMVAGAIPVFFWRRTAYYQYEWFLPGKPESYSVFIDRNA 431
            +S FCLQP+GDS+TRRS FD M+AG IPVFF   +AY QY W LP    +YSVFI  + 
Sbjct: 423 QSSLFCLQPQGDSYTRRSAFDSMLAGCIPVFFHPGSAYTQYTWHLPKNYTTYSVFIPEDD 482

Query: 432 VKNGTASIEAVLEKFSREEVRAMRDRVIESIPKFVYG-----TGAVRDAFDVAVDGVL 483
           V+    SIE  L +   ++V+ MR+ VI  IP+ +Y          +DAFDV+V  V+
Sbjct: 483 VRKRNISIEERLLQIPAKQVKIMRENVINLIPRLIYADPRSELETQKDAFDVSVQAVI 535

BLAST of Carg08460 vs. TAIR 10
Match: AT4G13990.1 (Exostosin family protein )

HSP 1 Score: 313.5 bits (802), Expect = 3.0e-85
Identity = 168/418 (40.19%), Postives = 246/418 (58.85%), Query Frame = 0

Query: 84  RFPSAAVSPASTDGDHCKNGRIFVYDLPTAFNEDIVENCDSLNPWSSS--CSAMVNGGFG 143
           RFP    SP S+    C    I+V++LP  FN D+++NC  +   +    C  + N GFG
Sbjct: 84  RFPDDP-SPDSS----CSGRYIYVHELPYRFNGDLLDNCFKITRGTEKDICPYIENYGFG 143

Query: 144 QRADSLAGVIPENLLASWYWTDQFVTEIIFHNRIMKHKCRVKEPESATAFYVPFYAGLAV 203
               +   V+   L  SW+ T+QF+ E+IFHN+++ ++C   +   A+A +VPFYAGL +
Sbjct: 144 PVIKNYENVL---LKQSWFTTNQFMLEVIFHNKMINYRCLTNDSSLASAVFVPFYAGLDM 203

Query: 204 GKFLWTASSAEDRDHHCRMILRWLSDQEYYKKSNGWDHFITMGRITWDFRRSSD--EDWG 263
            ++LW   +   RD     ++ WL  Q+ + + +G DHF+  GRI WDFRR +D   DWG
Sbjct: 204 SRYLW-GFNITVRDSSSHELMDWLVVQKEWGRMSGRDHFLVSGRIAWDFRRQTDNESDWG 263

Query: 264 SRCIYLPGMRNITRLLIERNPWDYFDVGVPYPTGFHPSSQDDMSAWQDFVRTRSRTHLFC 323
           S+  +LP  RN++ L IE + W   D  +PYPT FHP S D++  WQ+ +R+R R +LF 
Sbjct: 264 SKLRFLPESRNMSMLSIESSSWKN-DYAIPYPTCFHPRSVDEIVEWQELMRSRKREYLFT 323

Query: 324 FAGAKRAAFRNDFRGILLDQCRNSTAEKCRVVDC--AGSRCSNGTSAILESFLNSDFCLQ 383
           FAGA R  +++  RG ++D+C  S  ++C ++DC      C N  + +++ F NS FCLQ
Sbjct: 324 FAGAPRPEYKDSVRGKIIDECLES-KKQCYLLDCNYGNVNCDNPVN-VMKVFRNSVFCLQ 383

Query: 384 PRGDSFTRRSIFDCMVAGAIPVFFWRRTAYYQYEWFLPGKPESYSVFIDRNAVKNGTASI 443
           P GDS+TRRS+FD ++AG IPVFF   TAY QY+W LP    SYSV++    VK     I
Sbjct: 384 PPGDSYTRRSMFDSILAGCIPVFFHPGTAYAQYKWHLPKNHSSYSVYLPVKDVKEWNIKI 443

Query: 444 EAVLEKFSREEVRAMRDRVIESIPKFV-----YGTGAVRDAFDVAVDGVLRRFKEQEE 491
           +  L +   E V  +R+ VI  IPK V     YG+    DAF++AV G+L R +E  E
Sbjct: 444 KERLIEIPEERVVRLREEVIRLIPKVVYADPKYGSDGSEDAFELAVKGMLERIEEVRE 489

BLAST of Carg08460 vs. TAIR 10
Match: AT1G63450.1 (root hair specific 8 )

HSP 1 Score: 311.6 bits (797), Expect = 1.1e-84
Identity = 153/391 (39.13%), Postives = 227/391 (58.06%), Query Frame = 0

Query: 100 CKNGRIFVYDLPTAFNEDIVENCDSLNPWSSSCSAMVNGGFGQRADSLAGVIPENLLASW 159
           C+   ++VYDLP+ FN+D++  C  + PW+  C+   N  FG+  +S+           W
Sbjct: 279 CEGKGVYVYDLPSKFNKDLLRECSDMVPWADFCNYFKNDAFGELMESMG--------KGW 338

Query: 160 YWTDQFVTEIIFHNRIMKHKCRVKEPESATAFYVPFYAGLAVGKFLWTASSAEDRDHHCR 219
           + T Q+  E IFH+RI+KH CRV     A  FYVPFY G+ V ++ +   S++ +D    
Sbjct: 339 FRTHQYSLEPIFHSRILKHPCRVHNETQAKLFYVPFYGGMDVLRWHFKNVSSDVKDVLPI 398

Query: 220 MILRWLSDQEYYKKSNGWDHFITMGRITWDFRRSSDEDWGSRCIYLPGMRNITRLLIERN 279
            I++WL  ++ ++K++G DH   +G+I+WDFRR     WGS  + +  M+N T+LLIERN
Sbjct: 399 EIVKWLGSKKSWRKNSGKDHVFVLGKISWDFRRVDKYSWGSSLLEMQEMKNPTKLLIERN 458

Query: 280 PWDYFDVGVPYPTGFHPSSQDDMSAWQDFVRTRSRTHLFCFAGAKRAAFRNDFRGILLDQ 339
           PW+  D+ +P+PT FHP +  D++ WQ+ +  + R  L  FAGA R       R IL+DQ
Sbjct: 459 PWEVNDIAIPHPTYFHPKTDTDIAIWQNKILGKPRRSLISFAGAARPGNPESIRSILIDQ 518

Query: 340 CRNSTAEKCRVVDCAGSRCSNGTSAILESFLNSDFCLQPRGDSFTRRSIFDCMVAGAIPV 399
           CR S+  +CR ++C    C    S ++E F +S+FCLQP GDS TR+SIFD ++ G IPV
Sbjct: 519 CR-SSPNQCRFLNCTDGGCDKSES-VIELFRDSEFCLQPPGDSPTRKSIFDSLILGCIPV 578

Query: 400 FFWRRTAYYQYEWFLPGKPESYSVFIDRNAVKNGTAS-IEAVLEKFSREEVRAMRDRVIE 459
            F   +AYYQY W LP     YSV+I++  VK    + IE ++ K  RE        V E
Sbjct: 579 IFDPYSAYYQYTWHLPEDHRRYSVYINKEDVKLKRVNVIEKLMSKTLREREDMRSYIVHE 638

Query: 460 SIPKFVYGTG-----AVRDAFDVAVDGVLRR 485
            +P  VYG         RDAFD+ +D + ++
Sbjct: 639 LLPGLVYGDSNAKFERFRDAFDITMDSLFKK 659

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7035587.11.4e-299100.00Xyloglucan galactosyltransferase XLT2, partial [Cucurbita argyrosperma subsp. ar... [more]
KAG6605679.15.4e-29999.80Xyloglucan galactosyltransferase XLT2, partial [Cucurbita argyrosperma subsp. so... [more]
XP_023532626.13.0e-29798.99xyloglucan galactosyltransferase XLT2-like [Cucurbita pepo subsp. pepo][more]
XP_022957641.15.3e-29498.19xyloglucan galactosyltransferase XLT2-like [Cucurbita moschata][more]
XP_022996283.17.9e-29096.77xyloglucan galactosyltransferase XLT2-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
F4K6F13.2e-18560.84Xyloglucan galactosyltransferase XLT2 OS=Arabidopsis thaliana OX=3702 GN=XLT2 PE... [more]
O810722.2e-9340.40Probable xyloglucan galactosyltransferase GT11 OS=Arabidopsis thaliana OX=3702 G... [more]
Q8H0385.6e-8939.81Xyloglucan galactosyltransferase KATAMARI1 homolog OS=Oryza sativa subsp. japoni... [more]
Q7XJ987.3e-8939.23Xyloglucan galactosyltransferase MUR3 OS=Arabidopsis thaliana OX=3702 GN=MUR3 PE... [more]
Q84R164.2e-8440.19Probable xyloglucan galactosyltransferase GT14 OS=Arabidopsis thaliana OX=3702 G... [more]
Match NameE-valueIdentityDescription
A0A6J1GZT12.6e-29498.19xyloglucan galactosyltransferase XLT2-like OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1KAD23.8e-29096.77xyloglucan galactosyltransferase XLT2-like OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A6J1I2611.2e-25985.74xyloglucan galactosyltransferase XLT2-like OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A6J1EL911.6e-25986.32xyloglucan galactosyltransferase XLT2-like OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A5D3DAU56.6e-25885.37Xyloglucan galactosyltransferase XLT2 OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
Match NameE-valueIdentityDescription
AT5G62220.12.3e-18660.84glycosyltransferase 18 [more]
AT2G29040.11.6e-9440.40Exostosin family protein [more]
AT2G20370.15.2e-9039.23Exostosin family protein [more]
AT4G13990.13.0e-8540.19Exostosin family protein [more]
AT1G63450.11.1e-8439.13root hair specific 8 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR040911Exostosin, GT47 domainPFAMPF03016Exostosincoord: 100..439
e-value: 4.5E-77
score: 259.2
NoneNo IPR availablePANTHERPTHR11062:SF214XYLOGLUCAN GALACTOSYLTRANSFERASE XLT2coord: 24..490
IPR004263Exostosin-likePANTHERPTHR11062EXOSTOSIN HEPARAN SULFATE GLYCOSYLTRANSFERASE -RELATEDcoord: 24..490

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg08460-RACarg08460-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006486 protein glycosylation
biological_process GO:0009969 xyloglucan biosynthetic process
cellular_component GO:0000139 Golgi membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0008378 galactosyltransferase activity
molecular_function GO:0016757 glycosyltransferase activity