Homology
BLAST of Carg08456 vs. NCBI nr
Match:
KAG7035591.1 (Gibberellin 20 oxidase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 784.6 bits (2025), Expect = 3.7e-223
Identity = 374/374 (100.00%), Postives = 374/374 (100.00%), Query Frame = 0
Query: 1 MHVVTSTPESRHDGAPLVFDASVLRHQHNIPKQFIWPDEEKPAATCPELQVPLIDLSGFL 60
MHVVTSTPESRHDGAPLVFDASVLRHQHNIPKQFIWPDEEKPAATCPELQVPLIDLSGFL
Sbjct: 1 MHVVTSTPESRHDGAPLVFDASVLRHQHNIPKQFIWPDEEKPAATCPELQVPLIDLSGFL 60
Query: 61 SGEKDAAAEAVRLVGEACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLCQKQSAQRK 120
SGEKDAAAEAVRLVGEACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLCQKQSAQRK
Sbjct: 61 SGEKDAAAEAVRLVGEACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLCQKQSAQRK 120
Query: 121 AGEHCGYASSFTGRFSSKLPWKETLSFRFSADESFNNLVLHYLHDKLGDQFAKFGRVYQD 180
AGEHCGYASSFTGRFSSKLPWKETLSFRFSADESFNNLVLHYLHDKLGDQFAKFGRVYQD
Sbjct: 121 AGEHCGYASSFTGRFSSKLPWKETLSFRFSADESFNNLVLHYLHDKLGDQFAKFGRVYQD 180
Query: 181 YCEAMSGLSLGIMELLGKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCD 240
YCEAMSGLSLGIMELLGKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCD
Sbjct: 181 YCEAMSGLSLGIMELLGKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCD 240
Query: 241 PTSLTILHQDQVGGLQVFVDNQWRLITPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNS 300
PTSLTILHQDQVGGLQVFVDNQWRLITPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNS
Sbjct: 241 PTSLTILHQDQVGGLQVFVDNQWRLITPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNS 300
Query: 301 ERTRKSLAFFLCPRNDKVVRPPRELVDTQNPRRYPDFTWSMLLRFTQTHYRADMKTLEAF 360
ERTRKSLAFFLCPRNDKVVRPPRELVDTQNPRRYPDFTWSMLLRFTQTHYRADMKTLEAF
Sbjct: 301 ERTRKSLAFFLCPRNDKVVRPPRELVDTQNPRRYPDFTWSMLLRFTQTHYRADMKTLEAF 360
Query: 361 SAWLQQQQEQQFNI 375
SAWLQQQQEQQFNI
Sbjct: 361 SAWLQQQQEQQFNI 374
BLAST of Carg08456 vs. NCBI nr
Match:
KAG6605683.1 (Gibberellin 20 oxidase 1, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 776.2 bits (2003), Expect = 1.3e-220
Identity = 372/375 (99.20%), Postives = 373/375 (99.47%), Query Frame = 0
Query: 1 MHVVTSTPESRHDGAPLVFDASVLRHQHNIPKQFIWPDEEKPAATCPELQVPLIDLSGFL 60
MHVVT TPESRHDGAPLVFDASVLRHQHNIPKQFIWPDEEKPAATCPELQVPLIDLSGFL
Sbjct: 1 MHVVTFTPESRHDGAPLVFDASVLRHQHNIPKQFIWPDEEKPAATCPELQVPLIDLSGFL 60
Query: 61 SGEKDAAAEAVRLVGEACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLCQKQSAQRK 120
SGEKDAAAEAVRLVGEACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLCQKQSAQRK
Sbjct: 61 SGEKDAAAEAVRLVGEACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLCQKQSAQRK 120
Query: 121 AGEHCGYASSFTGRFSSKLPWKETLSFRFSADESFNNLVLHYLHDKLGDQFAKFGRVYQD 180
AGEHCGYASSFTGRFSSKLPWKETLSFRFSADESFNNLVLHYLHDKLGDQFAKFGRVYQ+
Sbjct: 121 AGEHCGYASSFTGRFSSKLPWKETLSFRFSADESFNNLVLHYLHDKLGDQFAKFGRVYQE 180
Query: 181 YCEAMSGLSLGIMELLGKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCD 240
YCEAMSGLSLGIMELLGKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCD
Sbjct: 181 YCEAMSGLSLGIMELLGKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCD 240
Query: 241 PTSLTILHQDQVGGLQVFVDNQWRLITPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNS 300
PTSLTILHQDQVGGLQVFVDNQWRLITPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNS
Sbjct: 241 PTSLTILHQDQVGGLQVFVDNQWRLITPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNS 300
Query: 301 ERTRKSLAFFLCPRNDKVVRPPRELVDTQNPRRYPDFTWSMLLRFTQTHYRADMKTLEAF 360
ERTRKSLAFFLCPRNDKVVRPPRELVDTQNPRRYPDFTWSMLLRFTQTHYRADMKTLEAF
Sbjct: 301 ERTRKSLAFFLCPRNDKVVRPPRELVDTQNPRRYPDFTWSMLLRFTQTHYRADMKTLEAF 360
Query: 361 SAWL-QQQQEQQFNI 375
SAWL QQQQEQQFNI
Sbjct: 361 SAWLQQQQQEQQFNI 375
BLAST of Carg08456 vs. NCBI nr
Match:
XP_022957867.1 (gibberellin 20 oxidase 1-like [Cucurbita moschata])
HSP 1 Score: 775.8 bits (2002), Expect = 1.7e-220
Identity = 369/371 (99.46%), Postives = 371/371 (100.00%), Query Frame = 0
Query: 1 MHVVTSTPESRHDGAPLVFDASVLRHQHNIPKQFIWPDEEKPAATCPELQVPLIDLSGFL 60
MHVVTSTPESRHDGAPLVFDASVLRHQHNIPKQFIWPDEEKPAATCPELQVPLIDLSGFL
Sbjct: 1 MHVVTSTPESRHDGAPLVFDASVLRHQHNIPKQFIWPDEEKPAATCPELQVPLIDLSGFL 60
Query: 61 SGEKDAAAEAVRLVGEACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLCQKQSAQRK 120
SGEKDAAAEAVRLVGEACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLCQKQSAQRK
Sbjct: 61 SGEKDAAAEAVRLVGEACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLCQKQSAQRK 120
Query: 121 AGEHCGYASSFTGRFSSKLPWKETLSFRFSADESFNNLVLHYLHDKLGDQFAKFGRVYQD 180
AGEHCGYASSFTGRFSSKLPWKETLSFRFSADESFNNLVLHYLHDKLGDQFAKFGRVYQD
Sbjct: 121 AGEHCGYASSFTGRFSSKLPWKETLSFRFSADESFNNLVLHYLHDKLGDQFAKFGRVYQD 180
Query: 181 YCEAMSGLSLGIMELLGKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCD 240
YCEAMSGLSLGIMELLGKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCD
Sbjct: 181 YCEAMSGLSLGIMELLGKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCD 240
Query: 241 PTSLTILHQDQVGGLQVFVDNQWRLITPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNS 300
PTSLTILHQDQVGGLQVFVDNQWRLITPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNS
Sbjct: 241 PTSLTILHQDQVGGLQVFVDNQWRLITPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNS 300
Query: 301 ERTRKSLAFFLCPRNDKVVRPPRELVDTQNPRRYPDFTWSMLLRFTQTHYRADMKTLEAF 360
ERTRKSLAFFLCPRNDKVVRPPRELVDTQNPRRYPDFTWSMLLRFTQTHYRADMKTLEAF
Sbjct: 301 ERTRKSLAFFLCPRNDKVVRPPRELVDTQNPRRYPDFTWSMLLRFTQTHYRADMKTLEAF 360
Query: 361 SAWLQQQQEQQ 372
SAWLQQQQ++Q
Sbjct: 361 SAWLQQQQQEQ 371
BLAST of Carg08456 vs. NCBI nr
Match:
XP_023533923.1 (gibberellin 20 oxidase 1-like [Cucurbita pepo subsp. pepo] >XP_023533924.1 gibberellin 20 oxidase 1-like [Cucurbita pepo subsp. pepo] >XP_023533925.1 gibberellin 20 oxidase 1-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 773.1 bits (1995), Expect = 1.1e-219
Identity = 371/382 (97.12%), Postives = 373/382 (97.64%), Query Frame = 0
Query: 1 MHVVTSTPESRHDGAPLVFDASVLRHQHNIPKQFIWPDEEKPAATCPELQVPLIDLSGFL 60
MHVVTSTPESRHDGAPLVFDASVLRHQHNIPKQFIWPDEEKPA TCPEL+VPLIDLSGFL
Sbjct: 1 MHVVTSTPESRHDGAPLVFDASVLRHQHNIPKQFIWPDEEKPATTCPELEVPLIDLSGFL 60
Query: 61 SGEKDAAAEAVRLVGEACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLCQKQSAQRK 120
SGEKDAAAEAVRLVGEACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLCQKQSAQRK
Sbjct: 61 SGEKDAAAEAVRLVGEACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLCQKQSAQRK 120
Query: 121 AGEHCGYASSFTGRFSSKLPWKETLSFRFSADESFNNLVLHYLHDKLGDQFAKFGRVYQD 180
AGEHCGYASSFTGRFSSKLPWKETLSFRFSADESFNNLVLHYLHDKLGDQFAKFGRVYQ+
Sbjct: 121 AGEHCGYASSFTGRFSSKLPWKETLSFRFSADESFNNLVLHYLHDKLGDQFAKFGRVYQE 180
Query: 181 YCEAMSGLSLGIMELLGKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCD 240
YCEAMSGLSLGIMELLGKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCD
Sbjct: 181 YCEAMSGLSLGIMELLGKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCD 240
Query: 241 PTSLTILHQDQVGGLQVFVDNQWRLITPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNS 300
PTSLTILHQDQVGGLQVFVDNQWRLITPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNS
Sbjct: 241 PTSLTILHQDQVGGLQVFVDNQWRLITPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNS 300
Query: 301 ERTRKSLAFFLCPRNDKVVRPPRELVDTQNPRRYPDFTWSMLLRFTQTHYRADMKTLEAF 360
ERTRKSLAFFLCPRNDKVVRPPRELVDTQNPRRYPDFTWSMLLRFTQTHYRADMKTLEAF
Sbjct: 301 ERTRKSLAFFLCPRNDKVVRPPRELVDTQNPRRYPDFTWSMLLRFTQTHYRADMKTLEAF 360
Query: 361 SAWLQQ--------QQEQQFNI 375
SAWLQQ QQEQQFNI
Sbjct: 361 SAWLQQQQQEQQQEQQEQQFNI 382
BLAST of Carg08456 vs. NCBI nr
Match:
XP_022996314.1 (gibberellin 20 oxidase 1-like [Cucurbita maxima] >CAC83626.1 gibberellin 20-oxidase [Cucurbita maxima])
HSP 1 Score: 763.1 bits (1969), Expect = 1.1e-216
Identity = 367/378 (97.09%), Postives = 371/378 (98.15%), Query Frame = 0
Query: 1 MHVVTSTPESRHDGAPLVFDASVLRHQHNIPKQFIWPDEEKPAATCPELQVPLIDLSGFL 60
MHVVTSTPE+RHDGAPLVFDASVLRHQHNIPKQFIWPDEEKPAATCPEL+VPLIDLSGFL
Sbjct: 1 MHVVTSTPEARHDGAPLVFDASVLRHQHNIPKQFIWPDEEKPAATCPELEVPLIDLSGFL 60
Query: 61 SGEKDAAAEAVRLVGEACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLCQKQSAQRK 120
SGEKDAAAEAVRLVGEACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPL QKQSAQRK
Sbjct: 61 SGEKDAAAEAVRLVGEACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLSQKQSAQRK 120
Query: 121 AGEHCGYASSFTGRFSSKLPWKETLSFRFSADESFNNLVLHYLHDKLGDQFAKFGRVYQD 180
AGEHCGYASSFTGRFSSKLPWKETLSFRF+ADES NNLVLHYL+DKLGDQFAKFGRVYQD
Sbjct: 121 AGEHCGYASSFTGRFSSKLPWKETLSFRFAADESLNNLVLHYLNDKLGDQFAKFGRVYQD 180
Query: 181 YCEAMSGLSLGIMELLGKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCD 240
YCEAMSGLSLGIMELLGKSLGVEEQ FKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCD
Sbjct: 181 YCEAMSGLSLGIMELLGKSLGVEEQCFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCD 240
Query: 241 PTSLTILHQDQVGGLQVFVDNQWRLITPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNS 300
PTSLTILHQDQVGGLQVFVDNQWRLITPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNS
Sbjct: 241 PTSLTILHQDQVGGLQVFVDNQWRLITPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNS 300
Query: 301 ERTRKSLAFFLCPRNDKVVRPPRELVDTQNPRRYPDFTWSMLLRFTQTHYRADMKTLEAF 360
ERTRKSLAFFLCPRNDKVVRPPRELVDTQNPRRYPDFTWSMLLRFTQTHYRADMKTLEAF
Sbjct: 301 ERTRKSLAFFLCPRNDKVVRPPRELVDTQNPRRYPDFTWSMLLRFTQTHYRADMKTLEAF 360
Query: 361 SAWLQQ----QQEQQFNI 375
SAWLQQ QQEQQFNI
Sbjct: 361 SAWLQQEQQEQQEQQFNI 378
BLAST of Carg08456 vs. ExPASy Swiss-Prot
Match:
Q39110 (Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=GA20OX1 PE=2 SV=2)
HSP 1 Score: 506.9 bits (1304), Expect = 1.9e-142
Identity = 242/349 (69.34%), Postives = 275/349 (78.80%), Query Frame = 0
Query: 16 PLVFDASVLRHQHNIPKQFIWPDEEKPAATCPELQVPLIDLSGFLSGEKDAAAEAVRLVG 75
PL+F+ S+L Q NIP QFIWPD+EKP+ EL VPLIDL LS + + +A RL+
Sbjct: 26 PLIFNPSMLNLQANIPNQFIWPDDEKPSINVLELDVPLIDLQNLLS-DPSSTLDASRLIS 85
Query: 76 EACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLCQKQSAQRKAGEHCGYASSFTGRF 135
EAC+KHGFFLVVNHG+ +LI +AH+Y FF++PL +KQ RK+GE GYASSFTGRF
Sbjct: 86 EACKKHGFFLVVNHGISEELISDAHEYTSRFFDMPLSEKQRVLRKSGESVGYASSFTGRF 145
Query: 136 SSKLPWKETLSFRFSADESFNNLVLHYLHDKLGDQFAKFGRVYQDYCEAMSGLSLGIMEL 195
S+KLPWKETLSFRF D S + V Y D LG F FG+VYQ+YCEAMS LSL IMEL
Sbjct: 146 STKLPWKETLSFRFCDDMSRSKSVQDYFCDALGHGFQPFGKVYQEYCEAMSSLSLKIMEL 205
Query: 196 LGKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSLTILHQDQVGGL 255
LG SLGV+ YF+ FF++NDSIMRLN+YPPC KP LTLGTGPHCDPTSLTILHQD V GL
Sbjct: 206 LGLSLGVKRDYFREFFEENDSIMRLNYYPPCIKPDLTLGTGPHCDPTSLTILHQDHVNGL 265
Query: 256 QVFVDNQWRLITPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNSERTRKSLAFFLCPRN 315
QVFV+NQWR I PN AFVVNIGDTFMALSN RYKSCLHRAVVNSE RKSLAFFLCP+
Sbjct: 266 QVFVENQWRSIRPNPKAFVVNIGDTFMALSNDRYKSCLHRAVVNSESERKSLAFFLCPKK 325
Query: 316 DKVVRPPRELVDTQNPRRYPDFTWSMLLRFTQTHYRADMKTLEAFSAWL 365
D+VV PPREL+D+ RRYPDFTWSM L FTQ HYRADM TL+AFS WL
Sbjct: 326 DRVVTPPRELLDSITSRRYPDFTWSMFLEFTQKHYRADMNTLQAFSDWL 373
BLAST of Carg08456 vs. ExPASy Swiss-Prot
Match:
Q39111 (Gibberellin 20 oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=GA20OX2 PE=2 SV=1)
HSP 1 Score: 505.0 bits (1299), Expect = 7.4e-142
Identity = 236/350 (67.43%), Postives = 274/350 (78.29%), Query Frame = 0
Query: 15 APLVFDASVLRHQHNIPKQFIWPDEEKPAATCPELQVPLIDLSGFLSGEKDAAAEAVRLV 74
+PL+F+ S+L Q IP QFIWPDEEKP+ PEL VP IDLS +D+ EA R++
Sbjct: 27 SPLIFNPSLLNLQSQIPNQFIWPDEEKPSIDIPELNVPFIDLS-----SQDSTLEAPRVI 86
Query: 75 GEACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLCQKQSAQRKAGEHCGYASSFTGR 134
EAC KHGFFLVVNHGV LI +AH+ M+ FF++PL KQ AQRK GE CGYASSFTGR
Sbjct: 87 AEACTKHGFFLVVNHGVSESLIADAHRLMESFFDMPLAGKQKAQRKPGESCGYASSFTGR 146
Query: 135 FSSKLPWKETLSFRFSADESFNNLVLHYLHDKLGDQFAKFGRVYQDYCEAMSGLSLGIME 194
FS+KLPWKETLSF+FS D S + V Y D LG +F +FG+VYQDYCEAMS LSL IME
Sbjct: 147 FSTKLPWKETLSFQFSNDNSGSRTVQDYFSDTLGQEFEQFGKVYQDYCEAMSSLSLKIME 206
Query: 195 LLGKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSLTILHQDQVGG 254
LLG SLGV YF+ FF++NDSIMRLN YPPCQ P LTLGTGPHCDP+SLTILHQD V G
Sbjct: 207 LLGLSLGVNRDYFRGFFEENDSIMRLNHYPPCQTPDLTLGTGPHCDPSSLTILHQDHVNG 266
Query: 255 LQVFVDNQWRLITPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNSERTRKSLAFFLCPR 314
LQVFVDNQW+ I PN AFVVNIGDTFMALSNG +KSCLHRAVVN E RKS+AFFLCP+
Sbjct: 267 LQVFVDNQWQSIRPNPKAFVVNIGDTFMALSNGIFKSCLHRAVVNRESARKSMAFFLCPK 326
Query: 315 NDKVVRPPRELVDTQNPRRYPDFTWSMLLRFTQTHYRADMKTLEAFSAWL 365
DKVV+PP ++++ R+YPDFTWSM L FTQ HYRAD+ TL++FS W+
Sbjct: 327 KDKVVKPPSDILEKMKTRKYPDFTWSMFLEFTQKHYRADVNTLDSFSNWV 371
BLAST of Carg08456 vs. ExPASy Swiss-Prot
Match:
Q39112 (Gibberellin 20 oxidase 3 OS=Arabidopsis thaliana OX=3702 GN=GA20OX3 PE=2 SV=1)
HSP 1 Score: 497.7 bits (1280), Expect = 1.2e-139
Identity = 235/355 (66.20%), Postives = 282/355 (79.44%), Query Frame = 0
Query: 18 VFDASVL-RHQHNIPKQFIWPDEEKPAATCPELQVPLIDLSGFLSGEKDAAAEAVRLVGE 77
+FDA +L +H H+IP+QF+WPD EKP+ LQVPLIDL+GFLSG+ A+EA RLV +
Sbjct: 24 IFDAKLLNQHSHHIPQQFVWPDHEKPSTDVQPLQVPLIDLAGFLSGDSCLASEATRLVSK 83
Query: 78 ACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLCQKQSAQRKAGEHCGYASSFTGRFS 137
A KHGFFL+ NHGVD L+ A+ +MD FF+ P C+KQ AQRK GE GYASSF GRFS
Sbjct: 84 AATKHGFFLITNHGVDESLLSRAYLHMDSFFKAPACEKQKAQRKWGESSGYASSFVGRFS 143
Query: 138 SKLPWKETLSFRFSADESFNN-LVLHYLHDKLGDQFAKFGRVYQDYCEAMSGLSLGIMEL 197
SKLPWKETLSF+FS +E ++ V ++ K+GD + FG+VYQ+Y EAM+ LSL IMEL
Sbjct: 144 SKLPWKETLSFKFSPEEKIHSQTVKDFVSKKMGDGYEDFGKVYQEYAEAMNTLSLKIMEL 203
Query: 198 LGKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSLTILHQDQVGGL 257
LG SLGVE +YFK FF+D+DSI RLN+YP C++P L LGTGPHCDPTSLTILHQDQVGGL
Sbjct: 204 LGMSLGVERRYFKEFFEDSDSIFRLNYYPQCKQPELALGTGPHCDPTSLTILHQDQVGGL 263
Query: 258 QVFVDNQWRLITPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNSERTRKSLAFFLCPRN 317
QVFVDN+W+ I PN AFVVNIGDTFMAL+NGRYKSCLHRAVVNSER RK+ AFFLCP+
Sbjct: 264 QVFVDNKWQSIPPNPHAFVVNIGDTFMALTNGRYKSCLHRAVVNSERERKTFAFFLCPKG 323
Query: 318 DKVVRPPRELVD--TQNPRRYPDFTWSMLLRFTQTHYRADMKTLEAFSAWLQQQQ 369
+KVV+PP ELV+ R+YPDFTWSM L FTQ HYRADM TL+ FS WL+ ++
Sbjct: 324 EKVVKPPEELVNGVKSGERKYPDFTWSMFLEFTQKHYRADMNTLDEFSIWLKNRR 378
BLAST of Carg08456 vs. ExPASy Swiss-Prot
Match:
O04705 (Gibberellin 20 oxidase 1-D OS=Triticum aestivum OX=4565 GN=GA20ox1D PE=1 SV=1)
HSP 1 Score: 479.6 bits (1233), Expect = 3.3e-134
Identity = 234/355 (65.92%), Postives = 268/355 (75.49%), Query Frame = 0
Query: 18 VFDASVLRHQHNIPKQFIWPDEEKPAA-TCPELQVPLIDLSGFLSGEKDAAAEAVRLVGE 77
VFDA+VL + +IP QFIWP+ E P EL VPLID+ G LSG+ AAAE RLVGE
Sbjct: 5 VFDAAVLSGRADIPSQFIWPEGESPTPDAAEELHVPLIDIGGMLSGDPAAAAEVTRLVGE 64
Query: 78 ACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLCQKQSAQRKAGEHCGYASSFTGRFS 137
ACE+HGFF VVNHG+D +L+ +AH+ +D FF +PL +KQ A R GE CGYASSFTGRF+
Sbjct: 65 ACERHGFFQVVNHGIDAELLADAHRCVDNFFTMPLPEKQRALRHPGESCGYASSFTGRFA 124
Query: 138 SKLPWKETLSFRFSADESFNNLVLHYLHDKLGDQFAKFGRVYQDYCEAMSGLSLGIMELL 197
SKLPWKETLSFR + LV+ Y+ LG+ + G VY YC MS LSL IME+L
Sbjct: 125 SKLPWKETLSFRSCPSDP--ALVVDYIVATLGEDHRRLGEVYARYCSEMSRLSLEIMEVL 184
Query: 198 GKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSLTILHQDQVGGLQ 257
G+SLGV +++ FF+ NDSIMRLN+YPPCQ+P TLGTGPHCDPTSLTILHQD VGGLQ
Sbjct: 185 GESLGVGRAHYRRFFEGNDSIMRLNYYPPCQRPLETLGTGPHCDPTSLTILHQDNVGGLQ 244
Query: 258 VFVDNQWRLITPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNSERTRKSLAFFLCPRND 317
V + +WR I P DAFVVNIGDTFMALSNGRYKSCLHRAVVNS RKSLAFFLCP D
Sbjct: 245 VHTEGRWRSIRPRADAFVVNIGDTFMALSNGRYKSCLHRAVVNSRVPRKSLAFFLCPEMD 304
Query: 318 KVVRPPRELVDTQNPRRYPDFTWSMLLRFTQTHYRADMKTLEAFSAWLQQQQEQQ 372
KVV PP LVD NPR YPDFTW LL FTQ HYRADMKTLE FS+W+ QQQ+ Q
Sbjct: 305 KVVAPPGTLVDAANPRAYPDFTWRSLLDFTQKHYRADMKTLEVFSSWIVQQQQPQ 357
BLAST of Carg08456 vs. ExPASy Swiss-Prot
Match:
O04707 (Gibberellin 20 oxidase 1-A OS=Triticum aestivum OX=4565 GN=GA20ox1A PE=2 SV=1)
HSP 1 Score: 479.2 bits (1232), Expect = 4.3e-134
Identity = 233/355 (65.63%), Postives = 269/355 (75.77%), Query Frame = 0
Query: 18 VFDASVLRHQHNIPKQFIWPDEEKPAA-TCPELQVPLIDLSGFLSGEKDAAAEAVRLVGE 77
VFDA+VL + +IP QFIWP+ E P EL VPLI++ G LSG+ AAAE RLVGE
Sbjct: 5 VFDAAVLSGRSDIPSQFIWPEGESPTPDAAEELHVPLINIGGMLSGDAAAAAEVTRLVGE 64
Query: 78 ACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLCQKQSAQRKAGEHCGYASSFTGRFS 137
ACE+HGFF VVNHG+D +L+ +AH+ +D FF +PL +KQ A R+ GE CGYASSFTGRF+
Sbjct: 65 ACERHGFFQVVNHGIDAELLADAHRCVDNFFTMPLPEKQRALRRPGESCGYASSFTGRFA 124
Query: 138 SKLPWKETLSFRFSADESFNNLVLHYLHDKLGDQFAKFGRVYQDYCEAMSGLSLGIMELL 197
SKLPWKETLSFR + LV+ Y+ LG+ + G VY YC MS LSL IME+L
Sbjct: 125 SKLPWKETLSFRSCPSDP--ALVVDYIVATLGEDHRRLGEVYARYCSEMSRLSLEIMEVL 184
Query: 198 GKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSLTILHQDQVGGLQ 257
G+SLGV +++ FF+ NDSIMRLN+YPPCQ+P TLGTGPHCDPTSLTILHQD VGGLQ
Sbjct: 185 GESLGVGRAHYRRFFEGNDSIMRLNYYPPCQRPLETLGTGPHCDPTSLTILHQDNVGGLQ 244
Query: 258 VFVDNQWRLITPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNSERTRKSLAFFLCPRND 317
V + +WR I P DAFVVNIGDTFMALSNGRYKSCLHRAVVNS RKSLAFFLCP D
Sbjct: 245 VHTEGRWRSIRPRADAFVVNIGDTFMALSNGRYKSCLHRAVVNSRVPRKSLAFFLCPEMD 304
Query: 318 KVVRPPRELVDTQNPRRYPDFTWSMLLRFTQTHYRADMKTLEAFSAWLQQQQEQQ 372
KVV PP LVD NPR YPDFTW LL FTQ HYRADMKTLE FS+W+ QQQ+ Q
Sbjct: 305 KVVAPPGTLVDASNPRAYPDFTWRSLLDFTQKHYRADMKTLEVFSSWIVQQQQGQ 357
BLAST of Carg08456 vs. ExPASy TrEMBL
Match:
A0A6J1H0C4 (gibberellin 20 oxidase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111459279 PE=3 SV=1)
HSP 1 Score: 775.8 bits (2002), Expect = 8.3e-221
Identity = 369/371 (99.46%), Postives = 371/371 (100.00%), Query Frame = 0
Query: 1 MHVVTSTPESRHDGAPLVFDASVLRHQHNIPKQFIWPDEEKPAATCPELQVPLIDLSGFL 60
MHVVTSTPESRHDGAPLVFDASVLRHQHNIPKQFIWPDEEKPAATCPELQVPLIDLSGFL
Sbjct: 1 MHVVTSTPESRHDGAPLVFDASVLRHQHNIPKQFIWPDEEKPAATCPELQVPLIDLSGFL 60
Query: 61 SGEKDAAAEAVRLVGEACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLCQKQSAQRK 120
SGEKDAAAEAVRLVGEACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLCQKQSAQRK
Sbjct: 61 SGEKDAAAEAVRLVGEACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLCQKQSAQRK 120
Query: 121 AGEHCGYASSFTGRFSSKLPWKETLSFRFSADESFNNLVLHYLHDKLGDQFAKFGRVYQD 180
AGEHCGYASSFTGRFSSKLPWKETLSFRFSADESFNNLVLHYLHDKLGDQFAKFGRVYQD
Sbjct: 121 AGEHCGYASSFTGRFSSKLPWKETLSFRFSADESFNNLVLHYLHDKLGDQFAKFGRVYQD 180
Query: 181 YCEAMSGLSLGIMELLGKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCD 240
YCEAMSGLSLGIMELLGKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCD
Sbjct: 181 YCEAMSGLSLGIMELLGKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCD 240
Query: 241 PTSLTILHQDQVGGLQVFVDNQWRLITPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNS 300
PTSLTILHQDQVGGLQVFVDNQWRLITPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNS
Sbjct: 241 PTSLTILHQDQVGGLQVFVDNQWRLITPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNS 300
Query: 301 ERTRKSLAFFLCPRNDKVVRPPRELVDTQNPRRYPDFTWSMLLRFTQTHYRADMKTLEAF 360
ERTRKSLAFFLCPRNDKVVRPPRELVDTQNPRRYPDFTWSMLLRFTQTHYRADMKTLEAF
Sbjct: 301 ERTRKSLAFFLCPRNDKVVRPPRELVDTQNPRRYPDFTWSMLLRFTQTHYRADMKTLEAF 360
Query: 361 SAWLQQQQEQQ 372
SAWLQQQQ++Q
Sbjct: 361 SAWLQQQQQEQ 371
BLAST of Carg08456 vs. ExPASy TrEMBL
Match:
Q8GT56 (Gibberellin 20-oxidase OS=Cucurbita maxima OX=3661 GN=ga20ox PE=2 SV=1)
HSP 1 Score: 763.1 bits (1969), Expect = 5.5e-217
Identity = 367/378 (97.09%), Postives = 371/378 (98.15%), Query Frame = 0
Query: 1 MHVVTSTPESRHDGAPLVFDASVLRHQHNIPKQFIWPDEEKPAATCPELQVPLIDLSGFL 60
MHVVTSTPE+RHDGAPLVFDASVLRHQHNIPKQFIWPDEEKPAATCPEL+VPLIDLSGFL
Sbjct: 1 MHVVTSTPEARHDGAPLVFDASVLRHQHNIPKQFIWPDEEKPAATCPELEVPLIDLSGFL 60
Query: 61 SGEKDAAAEAVRLVGEACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLCQKQSAQRK 120
SGEKDAAAEAVRLVGEACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPL QKQSAQRK
Sbjct: 61 SGEKDAAAEAVRLVGEACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLSQKQSAQRK 120
Query: 121 AGEHCGYASSFTGRFSSKLPWKETLSFRFSADESFNNLVLHYLHDKLGDQFAKFGRVYQD 180
AGEHCGYASSFTGRFSSKLPWKETLSFRF+ADES NNLVLHYL+DKLGDQFAKFGRVYQD
Sbjct: 121 AGEHCGYASSFTGRFSSKLPWKETLSFRFAADESLNNLVLHYLNDKLGDQFAKFGRVYQD 180
Query: 181 YCEAMSGLSLGIMELLGKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCD 240
YCEAMSGLSLGIMELLGKSLGVEEQ FKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCD
Sbjct: 181 YCEAMSGLSLGIMELLGKSLGVEEQCFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCD 240
Query: 241 PTSLTILHQDQVGGLQVFVDNQWRLITPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNS 300
PTSLTILHQDQVGGLQVFVDNQWRLITPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNS
Sbjct: 241 PTSLTILHQDQVGGLQVFVDNQWRLITPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNS 300
Query: 301 ERTRKSLAFFLCPRNDKVVRPPRELVDTQNPRRYPDFTWSMLLRFTQTHYRADMKTLEAF 360
ERTRKSLAFFLCPRNDKVVRPPRELVDTQNPRRYPDFTWSMLLRFTQTHYRADMKTLEAF
Sbjct: 301 ERTRKSLAFFLCPRNDKVVRPPRELVDTQNPRRYPDFTWSMLLRFTQTHYRADMKTLEAF 360
Query: 361 SAWLQQ----QQEQQFNI 375
SAWLQQ QQEQQFNI
Sbjct: 361 SAWLQQEQQEQQEQQFNI 378
BLAST of Carg08456 vs. ExPASy TrEMBL
Match:
K7ZU06 (Gibberellin 20-oxidase (Fragment) OS=Cucumis sativus OX=3659 GN=ga20ox1 PE=2 SV=1)
HSP 1 Score: 668.3 bits (1723), Expect = 1.9e-188
Identity = 320/372 (86.02%), Postives = 341/372 (91.67%), Query Frame = 0
Query: 1 MHVVTST---PESRHDGAPLVFDASVLRHQHNIPKQFIWPDEEKPA-ATCPELQVPLIDL 60
MHVVTS+ + +DG PLVFDASVLRHQH IPKQFIWPDEEKP+ A CPE++VPLIDL
Sbjct: 1 MHVVTSSMAKEAANNDGVPLVFDASVLRHQHKIPKQFIWPDEEKPSEAGCPEMEVPLIDL 60
Query: 61 SGFLSGEKDAAAEAVRLVGEACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLCQKQS 120
SGFLSG+KD+ EAVR VGEACEKHGFFLVVNHGVD KLI +AHKYM+EFFELPLC+KQ
Sbjct: 61 SGFLSGDKDSVREAVRHVGEACEKHGFFLVVNHGVDLKLIADAHKYMNEFFELPLCEKQR 120
Query: 121 AQRKAGEHCGYASSFTGRFSSKLPWKETLSFRFSADESFNNLVLHYLHDKLGDQFAKFGR 180
AQRK GEHCGYASSFTGRFSSKLPWKETLSFRFSADES ++ V HYL KLGD+FA FGR
Sbjct: 121 AQRKVGEHCGYASSFTGRFSSKLPWKETLSFRFSADESLDDHVFHYLRTKLGDRFANFGR 180
Query: 181 VYQDYCEAMSGLSLGIMELLGKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTG 240
VYQ+YCEAMSGLSLGIMELLGKSLGVE +YFKNFFKDNDSIMRLNFYPPCQKPHLTLGTG
Sbjct: 181 VYQEYCEAMSGLSLGIMELLGKSLGVEGEYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTG 240
Query: 241 PHCDPTSLTILHQDQVGGLQVFVDNQWRLITPNFDAFVVNIGDTFMALSNGRYKSCLHRA 300
PHCDPTSLTILHQDQVGGLQVFVDNQWRLI PNFDAFVVNIGDTFMALSNGRYKSCLHRA
Sbjct: 241 PHCDPTSLTILHQDQVGGLQVFVDNQWRLIAPNFDAFVVNIGDTFMALSNGRYKSCLHRA 300
Query: 301 VVNSERTRKSLAFFLCPRNDKVVRPPRELVDTQNPRRYPDFTWSMLLRFTQTHYRADMKT 360
VVNS+RTRKSLAFFLCPRNDKVV+PP ELVD +NPR YPDFTWSMLL+FTQ +YRADMKT
Sbjct: 301 VVNSKRTRKSLAFFLCPRNDKVVKPPMELVDNENPRIYPDFTWSMLLQFTQNNYRADMKT 360
Query: 361 LEAFSAWLQQQQ 369
LE FSA LQQQQ
Sbjct: 361 LEVFSASLQQQQ 372
BLAST of Carg08456 vs. ExPASy TrEMBL
Match:
A0A6J1I242 (gibberellin 20 oxidase 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111469145 PE=3 SV=1)
HSP 1 Score: 659.4 bits (1700), Expect = 8.6e-186
Identity = 308/358 (86.03%), Postives = 327/358 (91.34%), Query Frame = 0
Query: 9 ESRHDGAPLVFDASVLRHQHNIPKQFIWPDEEKPAATCPELQVPLIDLSGFLSGEKDAAA 68
E +DG PLVFDA+VLRH+ IPKQFIWPDE+KP CPEL+VPLIDLSGF+SG+KD+
Sbjct: 3 EGENDGVPLVFDAAVLRHEQKIPKQFIWPDEDKPIGECPELEVPLIDLSGFVSGDKDSVG 62
Query: 69 EAVRLVGEACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLCQKQSAQRKAGEHCGYA 128
EA+RLVGEAC+KHGFFLVVNHGVD KLI EAHKYM+EFF LPLC+KQ AQR+AGEHCGYA
Sbjct: 63 EAIRLVGEACQKHGFFLVVNHGVDSKLIAEAHKYMNEFFGLPLCEKQRAQRQAGEHCGYA 122
Query: 129 SSFTGRFSSKLPWKETLSFRFSADESFNNLVLHYLHDKLGDQFAKFGRVYQDYCEAMSGL 188
SSFTGRFSSKLPWKETLSFRFSAD+SF NLVL YL KLGDQF F RVYQ+YCEAMSGL
Sbjct: 123 SSFTGRFSSKLPWKETLSFRFSADDSFPNLVLDYLSAKLGDQFVDFSRVYQEYCEAMSGL 182
Query: 189 SLGIMELLGKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSLTILH 248
SLGIMELLGKSLGV+ YFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSLTILH
Sbjct: 183 SLGIMELLGKSLGVKGDYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSLTILH 242
Query: 249 QDQVGGLQVFVDNQWRLITPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNSERTRKSLA 308
QDQVGGLQVFVDNQWRLI PNFDAFV+NIGDTFMALSNGRYKSCLHRAVVN+E TRKSLA
Sbjct: 243 QDQVGGLQVFVDNQWRLIAPNFDAFVINIGDTFMALSNGRYKSCLHRAVVNNETTRKSLA 302
Query: 309 FFLCPRNDKVVRPPRELVDTQNPRRYPDFTWSMLLRFTQTHYRADMKTLEAFSAWLQQ 367
FFLCPRNDKVVRPPRELVDTQNPR YPDFTWSML FT HYRADMKTL+ FSAWLQQ
Sbjct: 303 FFLCPRNDKVVRPPRELVDTQNPRIYPDFTWSMLHHFTLNHYRADMKTLDVFSAWLQQ 360
BLAST of Carg08456 vs. ExPASy TrEMBL
Match:
A0A6J1EQF6 (gibberellin 20 oxidase 1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111435674 PE=3 SV=1)
HSP 1 Score: 656.0 bits (1691), Expect = 9.5e-185
Identity = 307/358 (85.75%), Postives = 327/358 (91.34%), Query Frame = 0
Query: 9 ESRHDGAPLVFDASVLRHQHNIPKQFIWPDEEKPAATCPELQVPLIDLSGFLSGEKDAAA 68
E +DG PLVFDA+VLRH+ IPKQFIWPDE+KP CPEL+VPLIDLSGF+SG+KD+
Sbjct: 3 EEGNDGVPLVFDAAVLRHEQKIPKQFIWPDEDKPTGQCPELEVPLIDLSGFVSGDKDSVG 62
Query: 69 EAVRLVGEACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLCQKQSAQRKAGEHCGYA 128
EA+RLVGEAC+KHGFFLVVNHGVD KLI EAHKYM+EFFELPLC+KQ AQR+AGEHCGYA
Sbjct: 63 EAIRLVGEACQKHGFFLVVNHGVDSKLIAEAHKYMNEFFELPLCEKQRAQRQAGEHCGYA 122
Query: 129 SSFTGRFSSKLPWKETLSFRFSADESFNNLVLHYLHDKLGDQFAKFGRVYQDYCEAMSGL 188
SSFTGRFSSKLPWKETLSFRFSAD+S NLVL YL KLGDQF F RVYQ+YCEAMSGL
Sbjct: 123 SSFTGRFSSKLPWKETLSFRFSADDSCPNLVLDYLSAKLGDQFVDFSRVYQEYCEAMSGL 182
Query: 189 SLGIMELLGKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSLTILH 248
SLGIMELLGKSLGVE YFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSLTILH
Sbjct: 183 SLGIMELLGKSLGVEGDYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSLTILH 242
Query: 249 QDQVGGLQVFVDNQWRLITPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNSERTRKSLA 308
QDQVGGLQVFVDNQWRLI PNFDAFV+NIGDTFMALSNGRYKSCLHRAVVN+E TRKSLA
Sbjct: 243 QDQVGGLQVFVDNQWRLIAPNFDAFVINIGDTFMALSNGRYKSCLHRAVVNNETTRKSLA 302
Query: 309 FFLCPRNDKVVRPPRELVDTQNPRRYPDFTWSMLLRFTQTHYRADMKTLEAFSAWLQQ 367
FFLCPRNDKVVRPPRELVDTQNPR YPDFTWSML FT +YRADMKTL+ FSAWL+Q
Sbjct: 303 FFLCPRNDKVVRPPRELVDTQNPRIYPDFTWSMLHHFTLNNYRADMKTLDVFSAWLRQ 360
BLAST of Carg08456 vs. TAIR 10
Match:
AT4G25420.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )
HSP 1 Score: 506.9 bits (1304), Expect = 1.4e-143
Identity = 242/349 (69.34%), Postives = 275/349 (78.80%), Query Frame = 0
Query: 16 PLVFDASVLRHQHNIPKQFIWPDEEKPAATCPELQVPLIDLSGFLSGEKDAAAEAVRLVG 75
PL+F+ S+L Q NIP QFIWPD+EKP+ EL VPLIDL LS + + +A RL+
Sbjct: 26 PLIFNPSMLNLQANIPNQFIWPDDEKPSINVLELDVPLIDLQNLLS-DPSSTLDASRLIS 85
Query: 76 EACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLCQKQSAQRKAGEHCGYASSFTGRF 135
EAC+KHGFFLVVNHG+ +LI +AH+Y FF++PL +KQ RK+GE GYASSFTGRF
Sbjct: 86 EACKKHGFFLVVNHGISEELISDAHEYTSRFFDMPLSEKQRVLRKSGESVGYASSFTGRF 145
Query: 136 SSKLPWKETLSFRFSADESFNNLVLHYLHDKLGDQFAKFGRVYQDYCEAMSGLSLGIMEL 195
S+KLPWKETLSFRF D S + V Y D LG F FG+VYQ+YCEAMS LSL IMEL
Sbjct: 146 STKLPWKETLSFRFCDDMSRSKSVQDYFCDALGHGFQPFGKVYQEYCEAMSSLSLKIMEL 205
Query: 196 LGKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSLTILHQDQVGGL 255
LG SLGV+ YF+ FF++NDSIMRLN+YPPC KP LTLGTGPHCDPTSLTILHQD V GL
Sbjct: 206 LGLSLGVKRDYFREFFEENDSIMRLNYYPPCIKPDLTLGTGPHCDPTSLTILHQDHVNGL 265
Query: 256 QVFVDNQWRLITPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNSERTRKSLAFFLCPRN 315
QVFV+NQWR I PN AFVVNIGDTFMALSN RYKSCLHRAVVNSE RKSLAFFLCP+
Sbjct: 266 QVFVENQWRSIRPNPKAFVVNIGDTFMALSNDRYKSCLHRAVVNSESERKSLAFFLCPKK 325
Query: 316 DKVVRPPRELVDTQNPRRYPDFTWSMLLRFTQTHYRADMKTLEAFSAWL 365
D+VV PPREL+D+ RRYPDFTWSM L FTQ HYRADM TL+AFS WL
Sbjct: 326 DRVVTPPRELLDSITSRRYPDFTWSMFLEFTQKHYRADMNTLQAFSDWL 373
BLAST of Carg08456 vs. TAIR 10
Match:
AT5G51810.1 (gibberellin 20 oxidase 2 )
HSP 1 Score: 505.0 bits (1299), Expect = 5.2e-143
Identity = 236/350 (67.43%), Postives = 274/350 (78.29%), Query Frame = 0
Query: 15 APLVFDASVLRHQHNIPKQFIWPDEEKPAATCPELQVPLIDLSGFLSGEKDAAAEAVRLV 74
+PL+F+ S+L Q IP QFIWPDEEKP+ PEL VP IDLS +D+ EA R++
Sbjct: 27 SPLIFNPSLLNLQSQIPNQFIWPDEEKPSIDIPELNVPFIDLS-----SQDSTLEAPRVI 86
Query: 75 GEACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLCQKQSAQRKAGEHCGYASSFTGR 134
EAC KHGFFLVVNHGV LI +AH+ M+ FF++PL KQ AQRK GE CGYASSFTGR
Sbjct: 87 AEACTKHGFFLVVNHGVSESLIADAHRLMESFFDMPLAGKQKAQRKPGESCGYASSFTGR 146
Query: 135 FSSKLPWKETLSFRFSADESFNNLVLHYLHDKLGDQFAKFGRVYQDYCEAMSGLSLGIME 194
FS+KLPWKETLSF+FS D S + V Y D LG +F +FG+VYQDYCEAMS LSL IME
Sbjct: 147 FSTKLPWKETLSFQFSNDNSGSRTVQDYFSDTLGQEFEQFGKVYQDYCEAMSSLSLKIME 206
Query: 195 LLGKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSLTILHQDQVGG 254
LLG SLGV YF+ FF++NDSIMRLN YPPCQ P LTLGTGPHCDP+SLTILHQD V G
Sbjct: 207 LLGLSLGVNRDYFRGFFEENDSIMRLNHYPPCQTPDLTLGTGPHCDPSSLTILHQDHVNG 266
Query: 255 LQVFVDNQWRLITPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNSERTRKSLAFFLCPR 314
LQVFVDNQW+ I PN AFVVNIGDTFMALSNG +KSCLHRAVVN E RKS+AFFLCP+
Sbjct: 267 LQVFVDNQWQSIRPNPKAFVVNIGDTFMALSNGIFKSCLHRAVVNRESARKSMAFFLCPK 326
Query: 315 NDKVVRPPRELVDTQNPRRYPDFTWSMLLRFTQTHYRADMKTLEAFSAWL 365
DKVV+PP ++++ R+YPDFTWSM L FTQ HYRAD+ TL++FS W+
Sbjct: 327 KDKVVKPPSDILEKMKTRKYPDFTWSMFLEFTQKHYRADVNTLDSFSNWV 371
BLAST of Carg08456 vs. TAIR 10
Match:
AT5G07200.1 (gibberellin 20-oxidase 3 )
HSP 1 Score: 497.7 bits (1280), Expect = 8.4e-141
Identity = 235/355 (66.20%), Postives = 282/355 (79.44%), Query Frame = 0
Query: 18 VFDASVL-RHQHNIPKQFIWPDEEKPAATCPELQVPLIDLSGFLSGEKDAAAEAVRLVGE 77
+FDA +L +H H+IP+QF+WPD EKP+ LQVPLIDL+GFLSG+ A+EA RLV +
Sbjct: 24 IFDAKLLNQHSHHIPQQFVWPDHEKPSTDVQPLQVPLIDLAGFLSGDSCLASEATRLVSK 83
Query: 78 ACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLCQKQSAQRKAGEHCGYASSFTGRFS 137
A KHGFFL+ NHGVD L+ A+ +MD FF+ P C+KQ AQRK GE GYASSF GRFS
Sbjct: 84 AATKHGFFLITNHGVDESLLSRAYLHMDSFFKAPACEKQKAQRKWGESSGYASSFVGRFS 143
Query: 138 SKLPWKETLSFRFSADESFNN-LVLHYLHDKLGDQFAKFGRVYQDYCEAMSGLSLGIMEL 197
SKLPWKETLSF+FS +E ++ V ++ K+GD + FG+VYQ+Y EAM+ LSL IMEL
Sbjct: 144 SKLPWKETLSFKFSPEEKIHSQTVKDFVSKKMGDGYEDFGKVYQEYAEAMNTLSLKIMEL 203
Query: 198 LGKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSLTILHQDQVGGL 257
LG SLGVE +YFK FF+D+DSI RLN+YP C++P L LGTGPHCDPTSLTILHQDQVGGL
Sbjct: 204 LGMSLGVERRYFKEFFEDSDSIFRLNYYPQCKQPELALGTGPHCDPTSLTILHQDQVGGL 263
Query: 258 QVFVDNQWRLITPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNSERTRKSLAFFLCPRN 317
QVFVDN+W+ I PN AFVVNIGDTFMAL+NGRYKSCLHRAVVNSER RK+ AFFLCP+
Sbjct: 264 QVFVDNKWQSIPPNPHAFVVNIGDTFMALTNGRYKSCLHRAVVNSERERKTFAFFLCPKG 323
Query: 318 DKVVRPPRELVD--TQNPRRYPDFTWSMLLRFTQTHYRADMKTLEAFSAWLQQQQ 369
+KVV+PP ELV+ R+YPDFTWSM L FTQ HYRADM TL+ FS WL+ ++
Sbjct: 324 EKVVKPPEELVNGVKSGERKYPDFTWSMFLEFTQKHYRADMNTLDEFSIWLKNRR 378
BLAST of Carg08456 vs. TAIR 10
Match:
AT1G60980.1 (gibberellin 20-oxidase 4 )
HSP 1 Score: 458.8 bits (1179), Expect = 4.3e-129
Identity = 221/354 (62.43%), Postives = 265/354 (74.86%), Query Frame = 0
Query: 18 VFDASVLRHQ-HNIPKQFIWPDEEKPAATCPELQVPLIDLSGFLSGEKDAAAEAVRLVGE 77
+FD++VL HQ +IP++F+WPD EKP+ P LQVP+IDL+GFLS + +EA RLV E
Sbjct: 23 IFDSTVLNHQPDHIPQEFVWPDHEKPSKNVPILQVPVIDLAGFLSNDPLLVSEAERLVSE 82
Query: 78 ACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLCQKQSAQRKAGEHCGYASSFTGRFS 137
A +KHGFFLV NHGVD +L+ AHK MD FF+ P +K AQRK GE GYASSF GRF
Sbjct: 83 AAKKHGFFLVTNHGVDERLLSTAHKLMDTFFKSPNYEKLKAQRKVGETTGYASSFVGRFK 142
Query: 138 SKLPWKETLSFRFS---ADESFNNLVLHYLHDKLGDQFAKFGRVYQDYCEAMSGLSLGIM 197
LPWKETLSF FS E+++ V +Y+ +GD + FG VYQ+Y E MS LSL IM
Sbjct: 143 ENLPWKETLSFSFSPTEKSENYSQTVKNYISKTMGDGYKDFGSVYQEYAETMSNLSLKIM 202
Query: 198 ELLGKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSLTILHQDQVG 257
ELLG SLG++ ++F+ FF+DN+SI RLN+YP C++P L LGTGPHCDPTSLTIL QDQV
Sbjct: 203 ELLGMSLGIKREHFREFFEDNESIFRLNYYPKCKQPDLVLGTGPHCDPTSLTILQQDQVS 262
Query: 258 GLQVFVDNQWRLITPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNSERTRKSLAFFLCP 317
GLQVFVDNQW+ I P A VVNIGDT MAL+NG YKSCLHRAVVN E TRK+LAFFLCP
Sbjct: 263 GLQVFVDNQWQSIPPIPQALVVNIGDTLMALTNGIYKSCLHRAVVNGETTRKTLAFFLCP 322
Query: 318 RNDKVVRPPRELVDTQNPRRYPDFTWSMLLRFTQTHYRADMKTLEAFSAWLQQQ 368
+ DKVV+PP EL + R YPDFTWSM L FT HYRADM TLE F+ WL+ +
Sbjct: 323 KVDKVVKPPSEL---EGERAYPDFTWSMFLEFTMKHYRADMNTLEEFTNWLKNK 373
BLAST of Carg08456 vs. TAIR 10
Match:
AT1G44090.1 (gibberellin 20-oxidase 5 )
HSP 1 Score: 335.1 bits (858), Expect = 7.2e-92
Identity = 168/366 (45.90%), Postives = 237/366 (64.75%), Query Frame = 0
Query: 8 PESRHDGAPLVFDASVLRHQHNIPKQFIWPDEEKPAATCPELQVPLIDLSGFLSGEKDAA 67
P+SR + V + S+L+ Q N+P +F WP E+ A + +L +P+IDLSGFL+G +
Sbjct: 22 PKSREMNSSDV-NFSLLQSQPNVPAEFFWP-EKDVAPSEGDLDLPIIDLSGFLNGNEAET 81
Query: 68 AEAVRLVGEACEKHGFFLVVNHGVDRKLIGEAHKYMDEFFELPLCQKQSAQRKAGEHCGY 127
A + V +AC HG FLVVNHG L +A + FF L +K A R G GY
Sbjct: 82 QLAAKAVKKACMAHGTFLVVNHGFKSGLAEKALEISSLFFGLSKDEKLRAYRIPGNISGY 141
Query: 128 ASSFTGRFSSKLPWKETLSFRFSADESFNNLVLHYLHDKLGDQFAKFGRVYQDYCEAMSG 187
+ + RFSS LPW ETL+ F ++V +L +LG+ + G+V+Q++C+AM+G
Sbjct: 142 TAGHSQRFSSNLPWNETLTLAFKKGPP--HVVEDFLTSRLGNHRQEIGQVFQEFCDAMNG 201
Query: 188 LSLGIMELLGKSLGVEEQ-YFKNFFKDNDSIMRLNFYPPCQKPHLTLGTGPHCDPTSLTI 247
L + +MELLG S+G++++ Y++ FF+D I R N+YPPC++P LG GPH DPT++T+
Sbjct: 202 LVMDLMELLGISMGLKDRTYYRRFFEDGSGIFRCNYYPPCKQPEKALGVGPHNDPTAITV 261
Query: 248 LHQDQVGGLQVFVDNQWRLITPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNSERTRKS 307
L QD V GL+VF W+ + P A VVN+GDTFMALSNG Y+SC HRAVVN E+ R+S
Sbjct: 262 LLQDDVVGLEVFAAGSWQTVRPRPGALVVNVGDTFMALSNGNYRSCYHRAVVNKEKVRRS 321
Query: 308 LAFFLCPRNDKVVRPPRELVDTQN-PRRYPDFTWSMLLRFTQTHYRADMKTLEAFSAWLQ 367
L FF CPR DK++ PP ELV+ + R+YPDFTW+ L +FTQ+ YR D TL FS+WL
Sbjct: 322 LVFFSCPREDKIIVPPPELVEGEEASRKYPDFTWAQLQKFTQSGYRVDNTTLHNFSSWLV 381
Query: 368 QQQEQQ 372
+++
Sbjct: 382 SNSDKK 383
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
KAG7035591.1 | 3.7e-223 | 100.00 | Gibberellin 20 oxidase 1, partial [Cucurbita argyrosperma subsp. argyrosperma] | [more] |
KAG6605683.1 | 1.3e-220 | 99.20 | Gibberellin 20 oxidase 1, partial [Cucurbita argyrosperma subsp. sororia] | [more] |
XP_022957867.1 | 1.7e-220 | 99.46 | gibberellin 20 oxidase 1-like [Cucurbita moschata] | [more] |
XP_023533923.1 | 1.1e-219 | 97.12 | gibberellin 20 oxidase 1-like [Cucurbita pepo subsp. pepo] >XP_023533924.1 gibbe... | [more] |
XP_022996314.1 | 1.1e-216 | 97.09 | gibberellin 20 oxidase 1-like [Cucurbita maxima] >CAC83626.1 gibberellin 20-oxid... | [more] |
Match Name | E-value | Identity | Description | |
Q39110 | 1.9e-142 | 69.34 | Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=GA20OX1 PE=2 SV=2 | [more] |
Q39111 | 7.4e-142 | 67.43 | Gibberellin 20 oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=GA20OX2 PE=2 SV=1 | [more] |
Q39112 | 1.2e-139 | 66.20 | Gibberellin 20 oxidase 3 OS=Arabidopsis thaliana OX=3702 GN=GA20OX3 PE=2 SV=1 | [more] |
O04705 | 3.3e-134 | 65.92 | Gibberellin 20 oxidase 1-D OS=Triticum aestivum OX=4565 GN=GA20ox1D PE=1 SV=1 | [more] |
O04707 | 4.3e-134 | 65.63 | Gibberellin 20 oxidase 1-A OS=Triticum aestivum OX=4565 GN=GA20ox1A PE=2 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1H0C4 | 8.3e-221 | 99.46 | gibberellin 20 oxidase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111459279 PE=3... | [more] |
Q8GT56 | 5.5e-217 | 97.09 | Gibberellin 20-oxidase OS=Cucurbita maxima OX=3661 GN=ga20ox PE=2 SV=1 | [more] |
K7ZU06 | 1.9e-188 | 86.02 | Gibberellin 20-oxidase (Fragment) OS=Cucumis sativus OX=3659 GN=ga20ox1 PE=2 SV=... | [more] |
A0A6J1I242 | 8.6e-186 | 86.03 | gibberellin 20 oxidase 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11146... | [more] |
A0A6J1EQF6 | 9.5e-185 | 85.75 | gibberellin 20 oxidase 1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111... | [more] |