Carg08446 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg08446
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	protein BOLA4, chloroplastic/mitochondrial
Location	Carg_Chr02: 5654598 .. 5658895 (-)
RNA-Seq Expression	Carg08446
Synteny	Carg08446

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TAAAATAATTGGTAGAGTCGAGGGTAAATTTATAACTCAGCCTGGGTGTTAAGCGTGTGCAGACCGTACATCAAAGATCAACCAAGAACTCGGTAGAACAAACCAATCAAGTAAGAGCAGTTCGATCGATTCAGCCGCGGCTAAGACTTCGACGATGATGATTAAGGTTCCGCAAGTCGCGTTCCGTCGGGTTCTTCCACTATTTGTTCACCGAAATGAACAGTTTTTTATTGTTTCCCAACAATTCACGACCAGCTGTGTCCGCACCGCCGCCGTTTCCGCTGCAATCACTACAACAACCGCCACTGGCGTCTGTAGTAAGAGTAGTAATGTTTTGAAATTTAGCTATGTTAATAGAAGATTCTCAAATTGGGGTTCAAGTCGGAACATGTGTGTTAGAGCCACTCAAGTGAATGACTCTGGATCTATCGATTCCCCGCTTATGCAGTCAATGGAGAACAAGGTAGTATATTGATTAGTTTTTTTTTTTTTTAATTTTCTTATTTTTATCGGTTTGGATTCATCTTTTTCTCGTTTTAGATTCATCTTTTTCTCGTTTTAGATAACTATTGCATATGCTCTTTGAGGAAATGCAGGGCAAGTTACTGAGCTAATTGAGAGAAATGGAATCTCCGAGTTGATTGTAGTTCAGAAGTGTTTTCTGTAGGACATAGAGAAAGAACTTAACGGCGATTATGTGTGATGATTTCACTTAATGTTTTGTTTATAACGTGCTGTTATTGTTGAACGATGAAAGTCCCACATCGGCTAATTTAGGGAATGTTCATGGGTTTATAATCAAAGAATACTCTCTCTATTGGAATGAGGCCTTTTGGGGAAGCCCAAAGCAAAGCCACGAGAGCTTATGCTCAAAGTGGACAATATCATATCATTGTGGAGAGTCGTATTATTTGTCTAACAGTTATCAAACTTATGCATTTCCGTTCTTTTCTACAAATGCATCTTTGTTGCGGCTTTGACGTAGTTTGGGTTTCTCGTTTCAATTTATAATATAGCAACCTGTTTAGCCCTAAGCTGAGCCGTTCATTAGACTAGAAGGCGGGCCAAAGGCACAACTAGTTAACTAATTCATCCCGAGGTCACCTGTGTTCAAACTGGCATATACAGAAATTGTTGGTATCAACCTTTATCATTCAAAGGAATCAAGTACCCTCTAACCATTGGTTCCAGGATGAGGGCCATTGCTGACGCAAAAGCCTTTCTAGGAAATCTAGGAATTCCGAGTGGTTGTTTGGACAATCAGTTACATTTATGTAACATCATTGCTTACATTGGCCTTGGCGCAAACAATTTTGAACAATCTACTAATTCATTATGTCTCTTTCCTTAAGCTCCCATGAAAGTCATGAAGGCTTTGAAACAATTGATGAGAGTTTCATTTGGAACAGGAACGTCTATAATCTTGCTTCTCATTGGGTGAAGTGGAACTTGTCTTCTCTTCCCACTGTGTATGACAATTTTGGATTGGGGTCCCTCAAATAGAGAAACAATGCTCGTCTCAAGAAGATGCAACTTTGGGGGAAGAGCCATGGCTACGGACTAGAAATCTTTTGGCTAGGTAAGTTGTCCAAAGGAAAAATATGATGTAGGTTGTGGTGTGACTTTGCTAGAGACATAGCCTTCTTTTAGTTCTTGTAGTTTAGAGTTGAAAATGGGTAGAGGATCTGTTTCTGGGAGGACAAGTGGTCTGACTTGGTTCTCTTTGCTCAGTTATTCTTCCATAATTATATGCTTTTTTAAAGAAAGGGTTTCTGCATCGAAATTTTGAAACTGGGACCAACAAGCTTGGAATTTAGGAGCGAAGAGGTTTTTTCAACCAATACTTTGGTAGTTGGTTGGCCCTCCTCGAGAAACTCTACATGGTCCATCTTGGGCTTCTCAAAGATAAAGCTTGTTGGTCCCTTGAATTGATGTGTACTTTCTCTTCCTAAAGCCTACTTGTTCGACTTCGAAGTTGAAATCCCTGTCAGTAAACCTTTTTTGGAATTTCAAGATTTCTAAACTGGTGAAGATGTTTCCGTGGTCCTTTCTTAACACTCGTGAAAAGCTAAAGGAAGTTCAATAATTGGGTTTTATCCCATTCACTATTTCCTCACTAAGAGGGTGAATCCATGGATCATCTCTTCCTTCATTGGTCCTGTACTTTGAGAGGTTGCTAGTATATTATGAATGAATACGGATTATCTCTTTGCATCTCTAGAAATGTAGATGAATGGTGGAGGGTTGGGCAGGTTGTTCTTTTAAAGGCAAGGTCAGATATTACAGAGTTGTGCGGCTAGGGCTCTCCTTTGCATCTTATGGAAGGAAAGAAATCAAAGGCATTTTAAAAAATAAACTATTTTATTTTTATTCTTTTTGCAATAGTGCGCAACTTACTGCATGTGGTGTTCAAATAATAGTAAATTCTTTTGTAATTACTCTTAGTTGATGATTATCTGTGACTGGAAAGAAATCAAAGGCATTTAAAAAAATAAACTATCTTATTTTTATTCTTTTTGCAACAGTGCGCAACATACTTCATGTGCTGTTCAAATAATAGTAAATTCTTTTGTAATTACTGTTAGTTGATGATTATCTGTGACTGGAAAGCTCTTATTTTGTAGCTATTTGGGTGGGGTTCTGTCTACCCAGCCTAAGCTGTTATTTTATGGCCTGGTTCTATTAGATCTTTTTGTTTCTTATCGTTTCTTATCCAAGGAAAAAAAAAATTGTTTCTTATGGTGGGATGCTAAATTAAGGATTAGGAACATAAGTGGAGATAAAGTTTAGTTTACAACATATTGTGGATGGGTGTGTTGTTCAACTGCTCATGAATTGGTATTTGAATGGCTCGGTAGTTTGTTTATTTTTTCGGGTCTATTTGTTTTCCTGCTCTCCAGCATTTCTTAATTTCTTATTGCACTCCTTCATGTTTTGAAATGAGCTCCATTTCTTGACATTAACTTGCTGAAGGGGCTAAAACATTCAAATTTCAACTGTTCTAAAAGGGTTAAATTATAGAAATGTACAGGTAATTCATCATCTTACTTAAAAGCTGTGCAATCATAGTAATGAATATTTCTTGAAATTATTCTGGTAAAACCTATTTGAACAGAAGTTGTTATTTCTTTCTTGAGTTTTTTCTTCCAATTTGTGGCTATTCTTTTTACATTTTCTTCATCTTTTTCAAGATGACTCAGTTTTTATTTATTGAGTTGACATTGCAAATTTCTCCATCAGATTAAGGAACAATTAGAAGCACAATCTGTATCGGTAAAAGATGCATATGGAGATGGACGACATGTTAGGTGAGGACTTTTTCCCTATAATTGCTTTTCTATGGTAGCATAGTGTAAGAAATTTGATTTATTAGAATGCCTTACTGATAAAATAGTTTATATACTTCAAAAAAAAATATATATCTTTTCTTCCACTTGTTCCGTAATCATGTTTGGGCTTCTTATAAAGCTTATACTCAAACACTCTTTTGGCATTTGTTTCTTGCCAAATCACAGGTTATGGGTTCGCTTTTCTTCAAATCCCTGATTTTTCAAGTTTAAACTTTGCATTTTTCATAAATATGGCCCCTCTCCTTCGGTTATTTGAAAACTCTCCCCTCTTGTAAATGATCGTTGTTGCAAACTTGAGTATACTCGGCCAAGTGGAAAGAGGTTTGGATTCCCACCTCCCCATGTTGATTTTGATTTTTTTTTTTTCCCCCCTTTTTGATATGCTTTCGTTCTTAAACTCCTGAGCCTCTAAGGCCAACTTCATCCCCTACGAATTGAGCAAATATCTTATCTCTAAATTACCTACTTGAACAACTAGTGGTGCATTCTTCTCAAAAAAGTTCATCGGGGGCATATTGGTCGGGGGAATTCATGTAGGATTCACGTTATGGTGAAGTACAATTGACGTCACAAGCCATATCCAAAGGACTAGTCAAGTTGAATTGATTGCTAATAAAGTTCATTTTGCTTGCAGCATTGATGTTGTTTCTTCTGCCTTTGAGGGACAAACGGCTGTTAATAGGCAGAGGATGGTGTACAAAGCTATTTGGGAGGAGCTACAGAGCACAGTCCATGCAGTGGATCAGATGACAACCAGAACACCCGCTGAAGCAGAAGCTCAGAACTCGTAACTCTAGAAGTTTGTCCAACCCTTATCTTTGCCAATAAATGTCTTACAATTAAAGTATGCACTTATTATTCCCTTTCAGCAATTGATTAATGGCACAAATCTTATGTTCGATCTTTCATTCAAGATTGGTGGTCAAGGAATCAACCTGTGTTTTTTTT

mRNA sequence

TAAAATAATTGGTAGAGTCGAGGGTAAATTTATAACTCAGCCTGGGTGTTAAGCGTGTGCAGACCGTACATCAAAGATCAACCAAGAACTCGGTAGAACAAACCAATCAAGTAAGAGCAGTTCGATCGATTCAGCCGCGGCTAAGACTTCGACGATGATGATTAAGGTTCCGCAAGTCGCGTTCCGTCGGGTTCTTCCACTATTTGTTCACCGAAATGAACAGTTTTTTATTGTTTCCCAACAATTCACGACCAGCTGTGTCCGCACCGCCGCCGTTTCCGCTGCAATCACTACAACAACCGCCACTGGCGTCTGTAGTAAGAGTAGTAATGTTTTGAAATTTAGCTATGTTAATAGAAGATTCTCAAATTGGGGTTCAAGTCGGAACATGTGTGTTAGAGCCACTCAAGTGAATGACTCTGGATCTATCGATTCCCCGCTTATGCAGTCAATGGAGAACAAGATTAAGGAACAATTAGAAGCACAATCTGTATCGGTAAAAGATGCATATGGAGATGGACGACATGTTAGCATTGATGTTGTTTCTTCTGCCTTTGAGGGACAAACGGCTGTTAATAGGCAGAGGATGGTGTACAAAGCTATTTGGGAGGAGCTACAGAGCACAGTCCATGCAGTGGATCAGATGACAACCAGAACACCCGCTGAAGCAGAAGCTCAGAACTCGTAACTCTAGAAGTTTGTCCAACCCTTATCTTTGCCAATAAATGTCTTACAATTAAAGTATGCACTTATTATTCCCTTTCAGCAATTGATTAATGGCACAAATCTTATGTTCGATCTTTCATTCAAGATTGGTGGTCAAGGAATCAACCTGTGTTTTTTTT

Coding sequence (CDS)

ATGATGATTAAGGTTCCGCAAGTCGCGTTCCGTCGGGTTCTTCCACTATTTGTTCACCGAAATGAACAGTTTTTTATTGTTTCCCAACAATTCACGACCAGCTGTGTCCGCACCGCCGCCGTTTCCGCTGCAATCACTACAACAACCGCCACTGGCGTCTGTAGTAAGAGTAGTAATGTTTTGAAATTTAGCTATGTTAATAGAAGATTCTCAAATTGGGGTTCAAGTCGGAACATGTGTGTTAGAGCCACTCAAGTGAATGACTCTGGATCTATCGATTCCCCGCTTATGCAGTCAATGGAGAACAAGATTAAGGAACAATTAGAAGCACAATCTGTATCGGTAAAAGATGCATATGGAGATGGACGACATGTTAGCATTGATGTTGTTTCTTCTGCCTTTGAGGGACAAACGGCTGTTAATAGGCAGAGGATGGTGTACAAAGCTATTTGGGAGGAGCTACAGAGCACAGTCCATGCAGTGGATCAGATGACAACCAGAACACCCGCTGAAGCAGAAGCTCAGAACTCGTAA

Protein sequence

MMIKVPQVAFRRVLPLFVHRNEQFFIVSQQFTTSCVRTAAVSAAITTTTATGVCSKSSNVLKFSYVNRRFSNWGSSRNMCVRATQVNDSGSIDSPLMQSMENKIKEQLEAQSVSVKDAYGDGRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAEAQNS

Homology

[Close]

BLAST of Carg08446 vs. NCBI nr
Match: KAG7035601.1 (Protein BOLA4, chloroplastic/mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 334.3 bits (856), Expect = 6.2e-88
Identity = 177/177 (100.00%), Postives = 177/177 (100.00%), Query Frame = 0


Query: 1   MMIKVPQVAFRRVLPLFVHRNEQFFIVSQQFTTSCVRTAAVSAAITTTTATGVCSKSSNV 60
           MMIKVPQVAFRRVLPLFVHRNEQFFIVSQQFTTSCVRTAAVSAAITTTTATGVCSKSSNV
Sbjct: 1   MMIKVPQVAFRRVLPLFVHRNEQFFIVSQQFTTSCVRTAAVSAAITTTTATGVCSKSSNV 60

Query: 61  LKFSYVNRRFSNWGSSRNMCVRATQVNDSGSIDSPLMQSMENKIKEQLEAQSVSVKDAYG 120
           LKFSYVNRRFSNWGSSRNMCVRATQVNDSGSIDSPLMQSMENKIKEQLEAQSVSVKDAYG
Sbjct: 61  LKFSYVNRRFSNWGSSRNMCVRATQVNDSGSIDSPLMQSMENKIKEQLEAQSVSVKDAYG 120

Query: 121 DGRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAEAQNS 178
           DGRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAEAQNS
Sbjct: 121 DGRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAEAQNS 177

[Close]

BLAST of Carg08446 vs. NCBI nr
Match: XP_022996142.1 (protein BOLA4, chloroplastic/mitochondrial [Cucurbita maxima])

HSP 1 Score: 327.4 bits (838), Expect = 7.6e-86
Identity = 173/176 (98.30%), Postives = 174/176 (98.86%), Query Frame = 0


Query: 2   MIKVPQVAFRRVLPLFVHRNEQFFIVSQQFTTSCVRTAAVSAAITTTTATGVCSKSSNVL 61
           MIKVPQVAFRRVLPLFVHRNEQFFIVSQQFTT+CVRTAAVSAAITTTTATGVCSKSSNVL
Sbjct: 6   MIKVPQVAFRRVLPLFVHRNEQFFIVSQQFTTNCVRTAAVSAAITTTTATGVCSKSSNVL 65

Query: 62  KFSYVNRRFSNWGSSRNMCVRATQVNDSGSIDSPLMQSMENKIKEQLEAQSVSVKDAYGD 121
           KFSYVN RFSNWGSSRNMCVRATQVNDSGSIDSPLMQSMENKIKEQLEAQSVSVKDAYGD
Sbjct: 66  KFSYVNGRFSNWGSSRNMCVRATQVNDSGSIDSPLMQSMENKIKEQLEAQSVSVKDAYGD 125

Query: 122 GRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAEAQNS 178
           GRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTP EAEAQNS
Sbjct: 126 GRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPTEAEAQNS 181

[Close]

BLAST of Carg08446 vs. NCBI nr
Match: XP_023534696.1 (protein BOLA4, chloroplastic/mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 320.1 bits (819), Expect = 1.2e-83
Identity = 170/176 (96.59%), Postives = 172/176 (97.73%), Query Frame = 0


Query: 2   MIKVPQVAFRRVLPLFVHRNEQFFIVSQQFTTSCVRTAAVSAAITTTTATGVCSKSSNVL 61
           MIK PQVAFRRVLPL VHRNEQFF+VSQQFTT+CVRTAAVSAAITTTTATGV SKSSNVL
Sbjct: 1   MIKAPQVAFRRVLPLIVHRNEQFFVVSQQFTTNCVRTAAVSAAITTTTATGVGSKSSNVL 60

Query: 62  KFSYVNRRFSNWGSSRNMCVRATQVNDSGSIDSPLMQSMENKIKEQLEAQSVSVKDAYGD 121
           KFSYVN RFSNWGSSRNMCVRATQVNDSGSIDSPLMQSMENKIKEQLEAQSVSVKDAYGD
Sbjct: 61  KFSYVNGRFSNWGSSRNMCVRATQVNDSGSIDSPLMQSMENKIKEQLEAQSVSVKDAYGD 120

Query: 122 GRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAEAQNS 178
           GRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAEAQNS
Sbjct: 121 GRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAEAQNS 176

[Close]

BLAST of Carg08446 vs. NCBI nr
Match: KAG6605693.1 (Protein BOLA4, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 314.7 bits (805), Expect = 5.1e-82
Identity = 170/176 (96.59%), Postives = 171/176 (97.16%), Query Frame = 0


Query: 2   MIKVPQVAFRRVLPLFVHRNEQFFIVSQQFTTSCVRTAAVSAAITTTTATGVCSKSSNVL 61
           MIKVPQVAFRRVLPLFVHRNEQF IVSQQFTT+CVRTAAVSAAITTTTAT V SKSSNVL
Sbjct: 1   MIKVPQVAFRRVLPLFVHRNEQFSIVSQQFTTNCVRTAAVSAAITTTTATCVRSKSSNVL 60

Query: 62  KFSYVNRRFSNWGSSRNMCVRATQVNDSGSIDSPLMQSMENKIKEQLEAQSVSVKDAYGD 121
           KFSYVN R SNWGSSRNMCVRATQVNDSGSIDSPLMQSMENKIKEQLEAQSVSVKDAYGD
Sbjct: 61  KFSYVNGRSSNWGSSRNMCVRATQVNDSGSIDSPLMQSMENKIKEQLEAQSVSVKDAYGD 120

Query: 122 GRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAEAQNS 178
           GRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAEAQNS
Sbjct: 121 GRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAEAQNS 176

[Close]

BLAST of Carg08446 vs. NCBI nr
Match: XP_022958680.1 (protein BOLA4, chloroplastic/mitochondrial [Cucurbita moschata])

HSP 1 Score: 311.2 bits (796), Expect = 5.6e-81
Identity = 169/176 (96.02%), Postives = 170/176 (96.59%), Query Frame = 0


Query: 2   MIKVPQVAFRRVLPLFVHRNEQFFIVSQQFTTSCVRTAAVSAAITTTTATGVCSKSSNVL 61
           MIKVPQVAFRRVLPLFVHRNEQF IVSQQFTT+CVRTAAVSAAITTTTAT V SKSSNVL
Sbjct: 1   MIKVPQVAFRRVLPLFVHRNEQFSIVSQQFTTNCVRTAAVSAAITTTTATCVRSKSSNVL 60

Query: 62  KFSYVNRRFSNWGSSRNMCVRATQVNDSGSIDSPLMQSMENKIKEQLEAQSVSVKDAYGD 121
           KFSYVN R SNWGSSRNMCVRATQVNDSGSIDSPLMQSMENKIKEQLEAQSVSVKDAYGD
Sbjct: 61  KFSYVNGRSSNWGSSRNMCVRATQVNDSGSIDSPLMQSMENKIKEQLEAQSVSVKDAYGD 120

Query: 122 GRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAEAQNS 178
           GRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAEAQ S
Sbjct: 121 GRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAEAQIS 176

[Close]

BLAST of Carg08446 vs. ExPASy Swiss-Prot
Match: Q9LF68 (Protein BOLA4, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=BOLA4 PE=1 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 2.9e-32
Identity = 79/127 (62.20%), Postives = 95/127 (74.80%), Query Frame = 0


Query: 51  TGVCSKSSNVLKFSYVNR--RFSNWGS----SRNMCVRATQVNDSGSIDSPLMQSMENKI 110
           + V S+        Y NR  R S +G+    S N   +++Q+ND+GSID  LMQSME KI
Sbjct: 50  SAVLSRKETAPSSIYGNRVSRVSGFGALDIRSLNFSTKSSQINDAGSIDQTLMQSMELKI 109

Query: 111 KEQLEAQSVSVKDAYGDGRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQM 170
           KEQL A+SV+V D  GDGRHV I+VVSSAFEGQ+AVNRQRMVYKAIWEELQ+ VHAVDQM
Sbjct: 110 KEQLNAESVTVTDMSGDGRHVCINVVSSAFEGQSAVNRQRMVYKAIWEELQNVVHAVDQM 169

Query: 171 TTRTPAE 172
           TT+TP+E
Sbjct: 170 TTKTPSE 176

[Close]

BLAST of Carg08446 vs. ExPASy Swiss-Prot
Match: Q682I1 (Protein BOLA1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BOLA1 PE=1 SV=1)

HSP 1 Score: 45.1 bits (105), Expect = 9.9e-04
Identity = 29/103 (28.16%), Postives = 57/103 (55.34%), Query Frame = 0


Query: 87  NDSGSIDSPLMQSMENKIKEQLEAQSVSVKDA-------------YGDGRHVSIDVVSSA 146
           +DSG+I++   + M  K++++LE   + ++D                D  H ++ +VS  
Sbjct: 60  SDSGAIENRASR-MREKLQKELEPVELVIEDVSYQHAGHAGMKGRTDDETHFNVKIVSKG 119

Query: 147 FEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAEAQN 177
           FEG   V R R+VY  + EEL + +HA+  + ++TP+E+ +++
Sbjct: 120 FEGMNLVKRHRLVYHLLREELDTGLHAL-SIVSKTPSESPSKD 160

[Close]

BLAST of Carg08446 vs. ExPASy TrEMBL
Match: A0A6J1K139 (protein BOLA4, chloroplastic/mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111491454 PE=3 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 3.7e-86
Identity = 173/176 (98.30%), Postives = 174/176 (98.86%), Query Frame = 0


Query: 2   MIKVPQVAFRRVLPLFVHRNEQFFIVSQQFTTSCVRTAAVSAAITTTTATGVCSKSSNVL 61
           MIKVPQVAFRRVLPLFVHRNEQFFIVSQQFTT+CVRTAAVSAAITTTTATGVCSKSSNVL
Sbjct: 6   MIKVPQVAFRRVLPLFVHRNEQFFIVSQQFTTNCVRTAAVSAAITTTTATGVCSKSSNVL 65

Query: 62  KFSYVNRRFSNWGSSRNMCVRATQVNDSGSIDSPLMQSMENKIKEQLEAQSVSVKDAYGD 121
           KFSYVN RFSNWGSSRNMCVRATQVNDSGSIDSPLMQSMENKIKEQLEAQSVSVKDAYGD
Sbjct: 66  KFSYVNGRFSNWGSSRNMCVRATQVNDSGSIDSPLMQSMENKIKEQLEAQSVSVKDAYGD 125

Query: 122 GRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAEAQNS 178
           GRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTP EAEAQNS
Sbjct: 126 GRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPTEAEAQNS 181

[Close]

BLAST of Carg08446 vs. ExPASy TrEMBL
Match: A0A6J1H464 (protein BOLA4, chloroplastic/mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111459834 PE=3 SV=1)

HSP 1 Score: 311.2 bits (796), Expect = 2.7e-81
Identity = 169/176 (96.02%), Postives = 170/176 (96.59%), Query Frame = 0


Query: 2   MIKVPQVAFRRVLPLFVHRNEQFFIVSQQFTTSCVRTAAVSAAITTTTATGVCSKSSNVL 61
           MIKVPQVAFRRVLPLFVHRNEQF IVSQQFTT+CVRTAAVSAAITTTTAT V SKSSNVL
Sbjct: 1   MIKVPQVAFRRVLPLFVHRNEQFSIVSQQFTTNCVRTAAVSAAITTTTATCVRSKSSNVL 60

Query: 62  KFSYVNRRFSNWGSSRNMCVRATQVNDSGSIDSPLMQSMENKIKEQLEAQSVSVKDAYGD 121
           KFSYVN R SNWGSSRNMCVRATQVNDSGSIDSPLMQSMENKIKEQLEAQSVSVKDAYGD
Sbjct: 61  KFSYVNGRSSNWGSSRNMCVRATQVNDSGSIDSPLMQSMENKIKEQLEAQSVSVKDAYGD 120

Query: 122 GRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAEAQNS 178
           GRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAEAQ S
Sbjct: 121 GRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAEAQIS 176

[Close]

BLAST of Carg08446 vs. ExPASy TrEMBL
Match: A0A0A0KRR5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G172860 PE=3 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 2.9e-67
Identity = 142/177 (80.23%), Postives = 156/177 (88.14%), Query Frame = 0


Query: 1   MMIKVPQVAFRRVLPLFVHRNEQFFIVSQQFTTSCVRTAAVSAAITTTTATGVCSKSSNV 60
           MMIKVPQVAFRRVLP+F+ RN  F +VS+Q T++CV T AV A I +T+ TG+ SKSSNV
Sbjct: 1   MMIKVPQVAFRRVLPIFLPRNRPFSVVSRQVTSNCVCTPAVVAVIISTSTTGLRSKSSNV 60

Query: 61  LKFSYVNRRFSNWGSSRNMCVRATQVNDSGSIDSPLMQSMENKIKEQLEAQSVSVKDAYG 120
            K+S  N  F  WGSSRN+ +RATQVN+SGSIDSPLMQSMENKIKEQL+AQSVSVKDAYG
Sbjct: 61  AKYSRANGTFPKWGSSRNLSIRATQVNESGSIDSPLMQSMENKIKEQLDAQSVSVKDAYG 120

Query: 121 DGRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAEAQNS 178
           DGRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEA AQNS
Sbjct: 121 DGRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAAAQNS 177

[Close]

BLAST of Carg08446 vs. ExPASy TrEMBL
Match: A0A1S3CT29 (protein BOLA4, chloroplastic/mitochondrial OS=Cucumis melo OX=3656 GN=LOC103504639 PE=3 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 1.1e-66
Identity = 140/176 (79.55%), Postives = 157/176 (89.20%), Query Frame = 0


Query: 2   MIKVPQVAFRRVLPLFVHRNEQFFIVSQQFTTSCVRTAAVSAAITTTTATGVCSKSSNVL 61
           MIKVPQ+AFRRVLP+ + RN+ F +VS+Q T++CV TAAV A IT+ + TG+ SKSSNV+
Sbjct: 3   MIKVPQLAFRRVLPMLLPRNQPFSVVSRQITSNCVCTAAVVAVITSKSNTGLRSKSSNVV 62

Query: 62  KFSYVNRRFSNWGSSRNMCVRATQVNDSGSIDSPLMQSMENKIKEQLEAQSVSVKDAYGD 121
           K+S  N  F  WGSSRN+ +RATQVN+SGSIDSPLMQSMENKIKEQL+AQSVSVKDAYGD
Sbjct: 63  KYSRANGTFPKWGSSRNLSIRATQVNESGSIDSPLMQSMENKIKEQLDAQSVSVKDAYGD 122

Query: 122 GRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAEAQNS 178
           GRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEA AQNS
Sbjct: 123 GRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAAAQNS 178

[Close]

BLAST of Carg08446 vs. ExPASy TrEMBL
Match: A0A5A7UJH5 (Protein BOLA4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G00900 PE=3 SV=1)

HSP 1 Score: 261.5 bits (667), Expect = 2.5e-66
Identity = 140/176 (79.55%), Postives = 156/176 (88.64%), Query Frame = 0


Query: 2   MIKVPQVAFRRVLPLFVHRNEQFFIVSQQFTTSCVRTAAVSAAITTTTATGVCSKSSNVL 61
           MIKVPQ+AFRRVLP+ + RN+ F +VS+Q T++CV TAAV A IT+ + TG+ SKSSNV+
Sbjct: 3   MIKVPQLAFRRVLPMLLPRNQPFSVVSRQITSNCVCTAAVVAVITSKSNTGLRSKSSNVV 62

Query: 62  KFSYVNRRFSNWGSSRNMCVRATQVNDSGSIDSPLMQSMENKIKEQLEAQSVSVKDAYGD 121
           K+S  N  F  WGSSRN  +RATQVN+SGSIDSPLMQSMENKIKEQL+AQSVSVKDAYGD
Sbjct: 63  KYSRANGTFPKWGSSRNSSIRATQVNESGSIDSPLMQSMENKIKEQLDAQSVSVKDAYGD 122

Query: 122 GRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAEAQNS 178
           GRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEA AQNS
Sbjct: 123 GRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAAAQNS 178

[Close]

BLAST of Carg08446 vs. TAIR 10
Match: AT5G17560.1 (BolA-like family protein )

HSP 1 Score: 139.8 bits (351), Expect = 2.1e-33
Identity = 79/127 (62.20%), Postives = 95/127 (74.80%), Query Frame = 0


Query: 51  TGVCSKSSNVLKFSYVNR--RFSNWGS----SRNMCVRATQVNDSGSIDSPLMQSMENKI 110
           + V S+        Y NR  R S +G+    S N   +++Q+ND+GSID  LMQSME KI
Sbjct: 50  SAVLSRKETAPSSIYGNRVSRVSGFGALDIRSLNFSTKSSQINDAGSIDQTLMQSMELKI 109

Query: 111 KEQLEAQSVSVKDAYGDGRHVSIDVVSSAFEGQTAVNRQRMVYKAIWEELQSTVHAVDQM 170
           KEQL A+SV+V D  GDGRHV I+VVSSAFEGQ+AVNRQRMVYKAIWEELQ+ VHAVDQM
Sbjct: 110 KEQLNAESVTVTDMSGDGRHVCINVVSSAFEGQSAVNRQRMVYKAIWEELQNVVHAVDQM 169

Query: 171 TTRTPAE 172
           TT+TP+E
Sbjct: 170 TTKTPSE 176

[Close]

BLAST of Carg08446 vs. TAIR 10
Match: AT1G55805.1 (BolA-like family protein )

HSP 1 Score: 45.1 bits (105), Expect = 7.0e-05
Identity = 29/103 (28.16%), Postives = 57/103 (55.34%), Query Frame = 0


Query: 87  NDSGSIDSPLMQSMENKIKEQLEAQSVSVKDA-------------YGDGRHVSIDVVSSA 146
           +DSG+I++   + M  K++++LE   + ++D                D  H ++ +VS  
Sbjct: 60  SDSGAIENRASR-MREKLQKELEPVELVIEDVSYQHAGHAGMKGRTDDETHFNVKIVSKG 119

Query: 147 FEGQTAVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAEAEAQN 177
           FEG   V R R+VY  + EEL + +HA+  + ++TP+E+ +++
Sbjct: 120 FEGMNLVKRHRLVYHLLREELDTGLHAL-SIVSKTPSESPSKD 160

[Close]

BLAST of Carg08446 vs. TAIR 10
Match: AT4G26500.1 (chloroplast sulfur E )

HSP 1 Score: 42.4 bits (98), Expect = 4.5e-04
Identity = 29/93 (31.18%), Postives = 53/93 (56.99%), Query Frame = 0


Query: 90  GSIDSPLMQSMENKIKE-QLEAQSVS--------VKDAYGDG--RHVSIDVVSSAFEGQT 149
           GS    + + +E ++   +LE + VS        V+ + GD    H ++ +VS AF+G++
Sbjct: 279 GSRGMRIREKLEKELDPVELEVEDVSYQHAGHAAVRGSAGDDGETHFNLRIVSDAFQGKS 338

Query: 150 AVNRQRMVYKAIWEELQSTVHAVDQMTTRTPAE 172
            V R R++Y  + +EL+S +HA+  +  +TPAE
Sbjct: 339 LVKRHRLIYDLLQDELKSGLHAL-SIVAKTPAE 370

The following BLAST results are available for this feature:

BLAST of Carg08446 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7035601.1	6.2e-88	100.00	Protein BOLA4, chloroplastic/mitochondrial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_022996142.1	7.6e-86	98.30	protein BOLA4, chloroplastic/mitochondrial [Cucurbita maxima]	[more]
XP_023534696.1	1.2e-83	96.59	protein BOLA4, chloroplastic/mitochondrial [Cucurbita pepo subsp. pepo]	[more]
KAG6605693.1	5.1e-82	96.59	Protein BOLA4, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subs...	[more]
XP_022958680.1	5.6e-81	96.02	protein BOLA4, chloroplastic/mitochondrial [Cucurbita moschata]	[more]

BLAST of Carg08446 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LF68	2.9e-32	62.20	Protein BOLA4, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=BO...	[more]
Q682I1	9.9e-04	28.16	Protein BOLA1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BOLA1 PE=1 SV=1	[more]

BLAST of Carg08446 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1K139	3.7e-86	98.30	protein BOLA4, chloroplastic/mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]
A0A6J1H464	2.7e-81	96.02	protein BOLA4, chloroplastic/mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A0A0KRR5	2.9e-67	80.23	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G172860 PE=3 SV=1	[more]
A0A1S3CT29	1.1e-66	79.55	protein BOLA4, chloroplastic/mitochondrial OS=Cucumis melo OX=3656 GN=LOC1035046...	[more]
A0A5A7UJH5	2.5e-66	79.55	Protein BOLA4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G00900 ...	[more]

BLAST of Carg08446 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G17560.1	2.1e-33	62.20	BolA-like family protein	[more]
AT1G55805.1	7.0e-05	28.16	BolA-like family protein	[more]
AT4G26500.1	4.5e-04	31.18	chloroplast sulfur E	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002634	BolA protein	PFAM	PF01722	BolA	coord: 119..171 e-value: 2.8E-13 score: 50.0
None	No IPR available	GENE3D	3.10.20.90		coord: 70..177 e-value: 1.5E-23 score: 85.1
None	No IPR available	PANTHER	PTHR46230	FAMILY NOT NAMED	coord: 24..175
None	No IPR available	PANTHER	PTHR46230:SF4	PROTEIN BOLA4, CHLOROPLASTIC/MITOCHONDRIAL	coord: 24..175
None	No IPR available	PROSITE	PS51257	PROKAR_LIPOPROTEIN	coord: 1..35 score: 5.0
IPR036065	BolA-like superfamily	SUPERFAMILY	82657	BolA-like	coord: 77..176

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg08446-RA	Carg08446-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0009507	chloroplast
cellular_component	GO:0005739	mitochondrion