Homology
BLAST of Carg08430 vs. NCBI nr
Match:
KAG7035615.1 (Valine--tRNA ligase, mitochondrial 1 [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 2230.3 bits (5778), Expect = 0.0e+00
Identity = 1106/1106 (100.00%), Postives = 1106/1106 (100.00%), Query Frame = 0
Query: 1 MRNGILNLLAVCVKQGSVLGEPLLVSLFSRQSYKLSCVHRAPFSSFSKGICPEFSVAPLK 60
MRNGILNLLAVCVKQGSVLGEPLLVSLFSRQSYKLSCVHRAPFSSFSKGICPEFSVAPLK
Sbjct: 1 MRNGILNLLAVCVKQGSVLGEPLLVSLFSRQSYKLSCVHRAPFSSFSKGICPEFSVAPLK 60
Query: 61 DMESVKKPEVEDPEKKKKKEEKAREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRG 120
DMESVKKPEVEDPEKKKKKEEKAREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRG
Sbjct: 61 DMESVKKPEVEDPEKKKKKEEKAREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRG 120
Query: 121 GDEENAEDFVDPDTPFGNKKLMSQQMSKQYNPSAVEKSWYEWWEKSGFFVADAKSSKPPF 180
GDEENAEDFVDPDTPFGNKKLMSQQMSKQYNPSAVEKSWYEWWEKSGFFVADAKSSKPPF
Sbjct: 121 GDEENAEDFVDPDTPFGNKKLMSQQMSKQYNPSAVEKSWYEWWEKSGFFVADAKSSKPPF 180
Query: 181 VIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIM 240
VIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIM
Sbjct: 181 VIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIM 240
Query: 241 RERNLTRHDIGREKFISEVWDWKTKYGGTILKQLRRLGASLDWTRECFTMDEKRSRAVTE 300
RERNLTRHDIGREKFISEVWDWKTKYGGTILKQLRRLGASLDWTRECFTMDEKRSRAVTE
Sbjct: 241 RERNLTRHDIGREKFISEVWDWKTKYGGTILKQLRRLGASLDWTRECFTMDEKRSRAVTE 300
Query: 301 AFVRLFKNGLIYRDLRLVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYEKPVEFGVLTS 360
AFVRLFKNGLIYRDLRLVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYEKPVEFGVLTS
Sbjct: 301 AFVRLFKNGLIYRDLRLVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYEKPVEFGVLTS 360
Query: 361 FAYPLEGELGEIVVATTRVETMLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPIICDA 420
FAYPLEGELGEIVVATTRVETMLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPIICDA
Sbjct: 361 FAYPLEGELGEIVVATTRVETMLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPIICDA 420
Query: 421 ILVDPNFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFEGMPRFKVR 480
ILVDPNFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFEGMPRFKVR
Sbjct: 421 ILVDPNFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFEGMPRFKVR 480
Query: 481 EMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAVMDNE 540
EMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAVMDNE
Sbjct: 481 EMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAVMDNE 540
Query: 541 CKKIEIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATLDDDQLKEFGAYNDHWV 600
CKKIEIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATLDDDQLKEFGAYNDHWV
Sbjct: 541 CKKIEIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATLDDDQLKEFGAYNDHWV 600
Query: 601 VARNEEEAEEEAKLIYAGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPDNTEDLKAFYPTS 660
VARNEEEAEEEAKLIYAGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPDNTEDLKAFYPTS
Sbjct: 601 VARNEEEAEEEAKLIYAGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPDNTEDLKAFYPTS 660
Query: 661 ALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVI 720
ALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVI
Sbjct: 661 ALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVI 720
Query: 721 NGISLEGLHKRLEEGNLDPKELAVAKEGQVKDFPNGISECGADALRFALISYTAQSDKIN 780
NGISLEGLHKRLEEGNLDPKELAVAKEGQVKDFPNGISECGADALRFALISYTAQSDKIN
Sbjct: 721 NGISLEGLHKRLEEGNLDPKELAVAKEGQVKDFPNGISECGADALRFALISYTAQSDKIN 780
Query: 781 LDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVPSKNVTPDALPFSCQWILSVLNKAISRT 840
LDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVPSKNVTPDALPFSCQWILSVLNKAISRT
Sbjct: 781 LDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVPSKNVTPDALPFSCQWILSVLNKAISRT 840
Query: 841 VSSLEAYEFSDATTAVYSWWQYQLCDVFIEAIKPYFSANDTEYASARSHAQDTLWLCLEN 900
VSSLEAYEFSDATTAVYSWWQYQLCDVFIEAIKPYFSANDTEYASARSHAQDTLWLCLEN
Sbjct: 841 VSSLEAYEFSDATTAVYSWWQYQLCDVFIEAIKPYFSANDTEYASARSHAQDTLWLCLEN 900
Query: 901 GLRLLHPFMPYVTEELWQRLPYPKSSTRPESIMICDYPSVTEEWTNESVETEMDLIVSAV 960
GLRLLHPFMPYVTEELWQRLPYPKSSTRPESIMICDYPSVTEEWTNESVETEMDLIVSAV
Sbjct: 901 GLRLLHPFMPYVTEELWQRLPYPKSSTRPESIMICDYPSVTEEWTNESVETEMDLIVSAV 960
Query: 961 KSLRSLAKESRERRPGYVLPRNDVVAETINKRKLEIVTLANLSSLTVINDNDAAPVGCAV 1020
KSLRSLAKESRERRPGYVLPRNDVVAETINKRKLEIVTLANLSSLTVINDNDAAPVGCAV
Sbjct: 961 KSLRSLAKESRERRPGYVLPRNDVVAETINKRKLEIVTLANLSSLTVINDNDAAPVGCAV 1020
Query: 1021 SVVNENLSVYLQFQGAISAEAELEKIKKKMEEVRKQQEKLKKMMDASGYKEKVRPHIHEE 1080
SVVNENLSVYLQFQGAISAEAELEKIKKKMEEVRKQQEKLKKMMDASGYKEKVRPHIHEE
Sbjct: 1021 SVVNENLSVYLQFQGAISAEAELEKIKKKMEEVRKQQEKLKKMMDASGYKEKVRPHIHEE 1080
Query: 1081 NVNKLASLMQELLSLEEAGQHIQAHR 1107
NVNKLASLMQELLSLEEAGQHIQAHR
Sbjct: 1081 NVNKLASLMQELLSLEEAGQHIQAHR 1106
BLAST of Carg08430 vs. NCBI nr
Match:
XP_023533939.1 (valine--tRNA ligase, mitochondrial 1 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 2222.6 bits (5758), Expect = 0.0e+00
Identity = 1100/1106 (99.46%), Postives = 1103/1106 (99.73%), Query Frame = 0
Query: 1 MRNGILNLLAVCVKQGSVLGEPLLVSLFSRQSYKLSCVHRAPFSSFSKGICPEFSVAPLK 60
MRNGILNLLAVCVKQG VLGEPLLVS FSRQSYKLSCVHRAPFSSFSKG+CPEFSVAPLK
Sbjct: 1 MRNGILNLLAVCVKQGRVLGEPLLVSFFSRQSYKLSCVHRAPFSSFSKGVCPEFSVAPLK 60
Query: 61 DMESVKKPEVEDPEKKKKKEEKAREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRG 120
DMESVKKPEVEDPEKKKKKEEKAREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRG
Sbjct: 61 DMESVKKPEVEDPEKKKKKEEKAREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRG 120
Query: 121 GDEENAEDFVDPDTPFGNKKLMSQQMSKQYNPSAVEKSWYEWWEKSGFFVADAKSSKPPF 180
GDEENAEDFVDPDTPFGNKKLMSQQMSKQYNPSAVEKSWYEWWEKSGFFVADAKSSKPPF
Sbjct: 121 GDEENAEDFVDPDTPFGNKKLMSQQMSKQYNPSAVEKSWYEWWEKSGFFVADAKSSKPPF 180
Query: 181 VIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIM 240
VIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIM
Sbjct: 181 VIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIM 240
Query: 241 RERNLTRHDIGREKFISEVWDWKTKYGGTILKQLRRLGASLDWTRECFTMDEKRSRAVTE 300
RERNLTRHDIGREKFISEVWDWKTKYGGTILKQLRRLGASLDWTRECFTMDEKRSRAVTE
Sbjct: 241 RERNLTRHDIGREKFISEVWDWKTKYGGTILKQLRRLGASLDWTRECFTMDEKRSRAVTE 300
Query: 301 AFVRLFKNGLIYRDLRLVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYEKPVEFGVLTS 360
AFVRLFKNGLIYRDLRLVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYEKPVEFGVLTS
Sbjct: 301 AFVRLFKNGLIYRDLRLVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYEKPVEFGVLTS 360
Query: 361 FAYPLEGELGEIVVATTRVETMLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPIICDA 420
FAYPLEGELGEIVVATTRVETMLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPIICDA
Sbjct: 361 FAYPLEGELGEIVVATTRVETMLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPIICDA 420
Query: 421 ILVDPNFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFEGMPRFKVR 480
ILVDPNFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFEGMPRFKVR
Sbjct: 421 ILVDPNFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFEGMPRFKVR 480
Query: 481 EMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAVMDNE 540
EMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAV DNE
Sbjct: 481 EMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAVTDNE 540
Query: 541 CKKIEIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATLDDDQLKEFGAYNDHWV 600
CKKIEIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATLDDDQLKEFGAYNDHWV
Sbjct: 541 CKKIEIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATLDDDQLKEFGAYNDHWV 600
Query: 601 VARNEEEAEEEAKLIYAGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPDNTEDLKAFYPTS 660
VARNEEEAEEEAKLIYAGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPDNTEDLKAFYPTS
Sbjct: 601 VARNEEEAEEEAKLIYAGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPDNTEDLKAFYPTS 660
Query: 661 ALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVI 720
ALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVI
Sbjct: 661 ALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVI 720
Query: 721 NGISLEGLHKRLEEGNLDPKELAVAKEGQVKDFPNGISECGADALRFALISYTAQSDKIN 780
NGISLEGLHKRLEEGNLDPKELAVAKEGQVKDFPNGISECGADALRFALISYTAQSDKIN
Sbjct: 721 NGISLEGLHKRLEEGNLDPKELAVAKEGQVKDFPNGISECGADALRFALISYTAQSDKIN 780
Query: 781 LDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVPSKNVTPDALPFSCQWILSVLNKAISRT 840
LDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVPSKNVTPDALPFSCQWILSVLNKAISRT
Sbjct: 781 LDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVPSKNVTPDALPFSCQWILSVLNKAISRT 840
Query: 841 VSSLEAYEFSDATTAVYSWWQYQLCDVFIEAIKPYFSANDTEYASARSHAQDTLWLCLEN 900
VSSLEAYEFSDATTAVYSWWQYQLCDVFIEAIKPYFS+NDTEYASARSHAQDTLWLCLEN
Sbjct: 841 VSSLEAYEFSDATTAVYSWWQYQLCDVFIEAIKPYFSSNDTEYASARSHAQDTLWLCLEN 900
Query: 901 GLRLLHPFMPYVTEELWQRLPYPKSSTRPESIMICDYPSVTEEWTNESVETEMDLIVSAV 960
GLRLLHPFMPYVTEELWQRLPYPKSSTRPESIMICDYPSVTEEWTNESVETEMDLIVSAV
Sbjct: 901 GLRLLHPFMPYVTEELWQRLPYPKSSTRPESIMICDYPSVTEEWTNESVETEMDLIVSAV 960
Query: 961 KSLRSLAKESRERRPGYVLPRNDVVAETINKRKLEIVTLANLSSLTVINDNDAAPVGCAV 1020
KSLRSLAKESRERRPGYVLPRND+VAETINKRKLEIVTLANLSSLTVINDNDAAPVGCAV
Sbjct: 961 KSLRSLAKESRERRPGYVLPRNDIVAETINKRKLEIVTLANLSSLTVINDNDAAPVGCAV 1020
Query: 1021 SVVNENLSVYLQFQGAISAEAELEKIKKKMEEVRKQQEKLKKMMDASGYKEKVRPHIHEE 1080
SVVNENLSVYLQFQGAISAEAELEKIKKKMEEVRKQQEKLKKMMDASGYKEKVRPHIHEE
Sbjct: 1021 SVVNENLSVYLQFQGAISAEAELEKIKKKMEEVRKQQEKLKKMMDASGYKEKVRPHIHEE 1080
Query: 1081 NVNKLASLMQELLSLEEAGQHIQAHR 1107
NVNKLASLMQELLSLEEAGQHIQAHR
Sbjct: 1081 NVNKLASLMQELLSLEEAGQHIQAHR 1106
BLAST of Carg08430 vs. NCBI nr
Match:
XP_022957954.1 (valine--tRNA ligase, mitochondrial 1 isoform X2 [Cucurbita moschata])
HSP 1 Score: 2220.7 bits (5753), Expect = 0.0e+00
Identity = 1100/1106 (99.46%), Postives = 1103/1106 (99.73%), Query Frame = 0
Query: 1 MRNGILNLLAVCVKQGSVLGEPLLVSLFSRQSYKLSCVHRAPFSSFSKGICPEFSVAPLK 60
MRNGILNLLAVCVKQG VLGEPLLVSLFSRQSYKLSCVHRAPFSSFSKGICPEFSVAPLK
Sbjct: 1 MRNGILNLLAVCVKQGRVLGEPLLVSLFSRQSYKLSCVHRAPFSSFSKGICPEFSVAPLK 60
Query: 61 DMESVKKPEVEDPEKKKKKEEKAREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRG 120
DMESVKKPEVEDPEKKKKKEEKAREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRG
Sbjct: 61 DMESVKKPEVEDPEKKKKKEEKAREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRG 120
Query: 121 GDEENAEDFVDPDTPFGNKKLMSQQMSKQYNPSAVEKSWYEWWEKSGFFVADAKSSKPPF 180
GDEENAEDFVDPDTPFG KKLMSQQMSKQYNPSAVEKSWYEWWEKSGFFVADAKSSKPPF
Sbjct: 121 GDEENAEDFVDPDTPFGKKKLMSQQMSKQYNPSAVEKSWYEWWEKSGFFVADAKSSKPPF 180
Query: 181 VIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIM 240
VIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIM
Sbjct: 181 VIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIM 240
Query: 241 RERNLTRHDIGREKFISEVWDWKTKYGGTILKQLRRLGASLDWTRECFTMDEKRSRAVTE 300
RERNLTRHDIGREKFISEVWDWKTKYGGTILKQLRRLGASLDWTRECFTMDEKRSRAVTE
Sbjct: 241 RERNLTRHDIGREKFISEVWDWKTKYGGTILKQLRRLGASLDWTRECFTMDEKRSRAVTE 300
Query: 301 AFVRLFKNGLIYRDLRLVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYEKPVEFGVLTS 360
AFVRLFKNGLIYRDLRLVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYEKPVEFGVLTS
Sbjct: 301 AFVRLFKNGLIYRDLRLVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYEKPVEFGVLTS 360
Query: 361 FAYPLEGELGEIVVATTRVETMLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPIICDA 420
FAYPLEGELGEIVVATTRVETMLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPIICDA
Sbjct: 361 FAYPLEGELGEIVVATTRVETMLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPIICDA 420
Query: 421 ILVDPNFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFEGMPRFKVR 480
ILVDPNFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFEGMPRF+VR
Sbjct: 421 ILVDPNFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFEGMPRFEVR 480
Query: 481 EMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAVMDNE 540
EMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAV DNE
Sbjct: 481 EMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAVTDNE 540
Query: 541 CKKIEIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATLDDDQLKEFGAYNDHWV 600
CKKIEIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATLDDDQLKEFGAYNDHWV
Sbjct: 541 CKKIEIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATLDDDQLKEFGAYNDHWV 600
Query: 601 VARNEEEAEEEAKLIYAGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPDNTEDLKAFYPTS 660
VARNEEEAEEEAKLIYAGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPDNTEDLKAFYPTS
Sbjct: 601 VARNEEEAEEEAKLIYAGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPDNTEDLKAFYPTS 660
Query: 661 ALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVI 720
ALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVI
Sbjct: 661 ALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVI 720
Query: 721 NGISLEGLHKRLEEGNLDPKELAVAKEGQVKDFPNGISECGADALRFALISYTAQSDKIN 780
NGISLEGLHKRLEEGNLDPKELAVAKEGQVKDFPNGISECGADALRFALISYTAQSDKIN
Sbjct: 721 NGISLEGLHKRLEEGNLDPKELAVAKEGQVKDFPNGISECGADALRFALISYTAQSDKIN 780
Query: 781 LDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVPSKNVTPDALPFSCQWILSVLNKAISRT 840
LDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVPSKNVTPDALPFSCQWILSVLNKAISRT
Sbjct: 781 LDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVPSKNVTPDALPFSCQWILSVLNKAISRT 840
Query: 841 VSSLEAYEFSDATTAVYSWWQYQLCDVFIEAIKPYFSANDTEYASARSHAQDTLWLCLEN 900
VSSLEAYEFSDATTAVYSWWQYQLCDVFIEAIKPYFS+NDTEYASARSHAQDTLWLCLEN
Sbjct: 841 VSSLEAYEFSDATTAVYSWWQYQLCDVFIEAIKPYFSSNDTEYASARSHAQDTLWLCLEN 900
Query: 901 GLRLLHPFMPYVTEELWQRLPYPKSSTRPESIMICDYPSVTEEWTNESVETEMDLIVSAV 960
GLRLLHPFMPYVTEELWQRLPYPKSSTRPESIMICDYPSVTEEWTNESVETEMDLIVSAV
Sbjct: 901 GLRLLHPFMPYVTEELWQRLPYPKSSTRPESIMICDYPSVTEEWTNESVETEMDLIVSAV 960
Query: 961 KSLRSLAKESRERRPGYVLPRNDVVAETINKRKLEIVTLANLSSLTVINDNDAAPVGCAV 1020
KSLRSLAKESRERRPGYVLPRNDVVAETINKRKLEIVTLANLSSLTVINDNDAAPVGCAV
Sbjct: 961 KSLRSLAKESRERRPGYVLPRNDVVAETINKRKLEIVTLANLSSLTVINDNDAAPVGCAV 1020
Query: 1021 SVVNENLSVYLQFQGAISAEAELEKIKKKMEEVRKQQEKLKKMMDASGYKEKVRPHIHEE 1080
SV+NENLSVYLQFQGAISAEAELEKIKKKMEEVRKQQEKLKKMMDASGYKEKVRPHIHEE
Sbjct: 1021 SVINENLSVYLQFQGAISAEAELEKIKKKMEEVRKQQEKLKKMMDASGYKEKVRPHIHEE 1080
Query: 1081 NVNKLASLMQELLSLEEAGQHIQAHR 1107
NVNKLASLMQELLSLEEAGQHIQAHR
Sbjct: 1081 NVNKLASLMQELLSLEEAGQHIQAHR 1106
BLAST of Carg08430 vs. NCBI nr
Match:
XP_022995352.1 (valine--tRNA ligase, mitochondrial 1 [Cucurbita maxima])
HSP 1 Score: 2208.3 bits (5721), Expect = 0.0e+00
Identity = 1097/1107 (99.10%), Postives = 1101/1107 (99.46%), Query Frame = 0
Query: 1 MRNGILNLLAVCVKQGSVLGEPLLVSLFSRQSYKLSCVHRAPFSSFSKGICP-EFSVAPL 60
MRNGILNLLAVCVKQG VLGEPLLVSLFSRQSYKLSCVHRAPFSSFSKGICP EFSVAP
Sbjct: 1 MRNGILNLLAVCVKQGRVLGEPLLVSLFSRQSYKLSCVHRAPFSSFSKGICPSEFSVAPH 60
Query: 61 KDMESVKKPEVEDPEKKKKKEEKAREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARR 120
KDMESVKKPEVEDPEKKKKKEEKAREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARR
Sbjct: 61 KDMESVKKPEVEDPEKKKKKEEKAREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARR 120
Query: 121 GGDEENAEDFVDPDTPFGNKKLMSQQMSKQYNPSAVEKSWYEWWEKSGFFVADAKSSKPP 180
GGDEENAEDFVDPDTPFG KKLMSQQMSKQYNPSAVEKSWYEWWEKSGFFVADAKSSKPP
Sbjct: 121 GGDEENAEDFVDPDTPFGKKKLMSQQMSKQYNPSAVEKSWYEWWEKSGFFVADAKSSKPP 180
Query: 181 FVIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKI 240
FVIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKI
Sbjct: 181 FVIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKI 240
Query: 241 MRERNLTRHDIGREKFISEVWDWKTKYGGTILKQLRRLGASLDWTRECFTMDEKRSRAVT 300
MRERNLTRHDIGREKFISEVWDWKTKYGGTILKQLRRLGASLDWTRECFTMDEKRSRAVT
Sbjct: 241 MRERNLTRHDIGREKFISEVWDWKTKYGGTILKQLRRLGASLDWTRECFTMDEKRSRAVT 300
Query: 301 EAFVRLFKNGLIYRDLRLVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYEKPVEFGVLT 360
EAFVRLFKNGLIYRDLRLVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYEKPVEFGVLT
Sbjct: 301 EAFVRLFKNGLIYRDLRLVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYEKPVEFGVLT 360
Query: 361 SFAYPLEGELGEIVVATTRVETMLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPIICD 420
SFAYPLEGELGEIVVATTRVETMLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPIICD
Sbjct: 361 SFAYPLEGELGEIVVATTRVETMLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPIICD 420
Query: 421 AILVDPNFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFEGMPRFKV 480
AILVDPNFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFEGMPRFKV
Sbjct: 421 AILVDPNFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFEGMPRFKV 480
Query: 481 REMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAVMDN 540
REMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAV DN
Sbjct: 481 REMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAVTDN 540
Query: 541 ECKKIEIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATLDDDQLKEFGAYNDHW 600
ECKKI+IIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATLDDDQLKEFGAYNDHW
Sbjct: 541 ECKKIKIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATLDDDQLKEFGAYNDHW 600
Query: 601 VVARNEEEAEEEAKLIYAGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPDNTEDLKAFYPT 660
VVARNEEEAEEEAKLIYAGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPDNTEDLKAFYPT
Sbjct: 601 VVARNEEEAEEEAKLIYAGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPDNTEDLKAFYPT 660
Query: 661 SALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEV 720
SALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEV
Sbjct: 661 SALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEV 720
Query: 721 INGISLEGLHKRLEEGNLDPKELAVAKEGQVKDFPNGISECGADALRFALISYTAQSDKI 780
INGISLEGLHKRLEEGNLDPKELAVAKEGQVKDFPNGISECGADALRFALISYTAQSDKI
Sbjct: 721 INGISLEGLHKRLEEGNLDPKELAVAKEGQVKDFPNGISECGADALRFALISYTAQSDKI 780
Query: 781 NLDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVPSKNVTPDALPFSCQWILSVLNKAISR 840
NLDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVPSKNVTPDALPFSCQWILSVLNKAISR
Sbjct: 781 NLDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVPSKNVTPDALPFSCQWILSVLNKAISR 840
Query: 841 TVSSLEAYEFSDATTAVYSWWQYQLCDVFIEAIKPYFSANDTEYASARSHAQDTLWLCLE 900
TVSSLEAYEFSDATTAVY WWQYQLCDVFIEAIKPYFS+NDTEYASARSHAQDTLWLCLE
Sbjct: 841 TVSSLEAYEFSDATTAVYYWWQYQLCDVFIEAIKPYFSSNDTEYASARSHAQDTLWLCLE 900
Query: 901 NGLRLLHPFMPYVTEELWQRLPYPKSSTRPESIMICDYPSVTEEWTNESVETEMDLIVSA 960
NGLRLLHPFMPYVTEELWQRLPYPKSSTRPESIMICDYPSVTEEWT+ESVETEMDLIVSA
Sbjct: 901 NGLRLLHPFMPYVTEELWQRLPYPKSSTRPESIMICDYPSVTEEWTSESVETEMDLIVSA 960
Query: 961 VKSLRSLAKESRERRPGYVLPRNDVVAETINKRKLEIVTLANLSSLTVINDNDAAPVGCA 1020
VKSLRSLAKESRERRPGY+LPRNDVVAETINKRKLEIVTLANLSSLTVINDNDAAPVGCA
Sbjct: 961 VKSLRSLAKESRERRPGYMLPRNDVVAETINKRKLEIVTLANLSSLTVINDNDAAPVGCA 1020
Query: 1021 VSVVNENLSVYLQFQGAISAEAELEKIKKKMEEVRKQQEKLKKMMDASGYKEKVRPHIHE 1080
VSVVNENLSVYLQFQGAISAEAELEKIKKKMEEVRKQQEKLKKMMDASGYKEKVRPHIHE
Sbjct: 1021 VSVVNENLSVYLQFQGAISAEAELEKIKKKMEEVRKQQEKLKKMMDASGYKEKVRPHIHE 1080
Query: 1081 ENVNKLASLMQELLSLEEAGQHIQAHR 1107
ENVNKLASLMQELLSLEEAGQHIQAHR
Sbjct: 1081 ENVNKLASLMQELLSLEEAGQHIQAHR 1107
BLAST of Carg08430 vs. NCBI nr
Match:
KAG6605705.1 (Valine--tRNA ligase, mitochondrial 1, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 2173.3 bits (5630), Expect = 0.0e+00
Identity = 1074/1076 (99.81%), Postives = 1076/1076 (100.00%), Query Frame = 0
Query: 31 QSYKLSCVHRAPFSSFSKGICPEFSVAPLKDMESVKKPEVEDPEKKKKKEEKAREKELKK 90
QSYKLSCVHRAPFSSFSKGICPEFSVAPLKDMESVKKPEVEDPEKKKKKEEKAREKELKK
Sbjct: 13 QSYKLSCVHRAPFSSFSKGICPEFSVAPLKDMESVKKPEVEDPEKKKKKEEKAREKELKK 72
Query: 91 KKALEKALKLQAQQTSNAPKKSEKKNARRGGDEENAEDFVDPDTPFGNKKLMSQQMSKQY 150
KKALEKALKLQAQQTSNAPKKSEKKNARRGGDEENAEDFVDPDTPFGNKKLMSQQMSKQY
Sbjct: 73 KKALEKALKLQAQQTSNAPKKSEKKNARRGGDEENAEDFVDPDTPFGNKKLMSQQMSKQY 132
Query: 151 NPSAVEKSWYEWWEKSGFFVADAKSSKPPFVIVLPPPNVTGALHIGHALTAAIEDAIIRW 210
NPSAVEKSWYEWWEKSGFFVADAKSSKPPFVIVLPPPNVTGALHIGHALTAAIEDAIIRW
Sbjct: 133 NPSAVEKSWYEWWEKSGFFVADAKSSKPPFVIVLPPPNVTGALHIGHALTAAIEDAIIRW 192
Query: 211 RRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKFISEVWDWKTKYGGTI 270
RRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKFISEVWDWKTKYGGTI
Sbjct: 193 RRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKFISEVWDWKTKYGGTI 252
Query: 271 LKQLRRLGASLDWTRECFTMDEKRSRAVTEAFVRLFKNGLIYRDLRLVNWDCVLRTAISD 330
LKQLRRLGASLDWTRECFTMDEKRSRAVTEAFVRLFKNGLIYRDLRLVNWDCVLRTAISD
Sbjct: 253 LKQLRRLGASLDWTRECFTMDEKRSRAVTEAFVRLFKNGLIYRDLRLVNWDCVLRTAISD 312
Query: 331 IEVDYIDIKEKTLLKVPGYEKPVEFGVLTSFAYPLEGELGEIVVATTRVETMLGDTAIAI 390
IEVDYIDIKEKTLLKVPGYEKPVEFGVLTSFAYPLEGELGEIVVATTRVETMLGDTAIAI
Sbjct: 313 IEVDYIDIKEKTLLKVPGYEKPVEFGVLTSFAYPLEGELGEIVVATTRVETMLGDTAIAI 372
Query: 391 HPDDTRYKHLHGKFAIHPFNGRKLPIICDAILVDPNFGTGAVKITPAHDPNDFEVGKRHN 450
HPDDTRYKHLHGKFAIHPFNGRKLPIICDAILVDPNFGTGAVKITPAHDPNDFEVGKRHN
Sbjct: 373 HPDDTRYKHLHGKFAIHPFNGRKLPIICDAILVDPNFGTGAVKITPAHDPNDFEVGKRHN 432
Query: 451 LEFINIFTDDGKINSNGGSEFEGMPRFKVREMVVDALQKKGLYRGAKDNEMRLGLCSRSN 510
LEFINIFTDDGKINSNGGSEFEGMPRFKVREMVVDALQKKGLYRGAKDNEMRLGLCSRSN
Sbjct: 433 LEFINIFTDDGKINSNGGSEFEGMPRFKVREMVVDALQKKGLYRGAKDNEMRLGLCSRSN 492
Query: 511 DVVEPMIKPQWYVNCKDMAKQSLDAVMDNECKKIEIIPKQYSADWKRWLENIRDWCISRQ 570
DVVEPMIKPQWYVNCKDMAKQSLDAVMDNECKKIEIIPKQYSADWKRWLENIRDWCISRQ
Sbjct: 493 DVVEPMIKPQWYVNCKDMAKQSLDAVMDNECKKIEIIPKQYSADWKRWLENIRDWCISRQ 552
Query: 571 LWWGHRIPAWYATLDDDQLKEFGAYNDHWVVARNEEEAEEEAKLIYAGKKFHLTQDPDVL 630
LWWGHRIPAWYATLDDDQLKEFGAYNDHWVVARNEEEAEEEAKLIYAGKKFHLTQDPDVL
Sbjct: 553 LWWGHRIPAWYATLDDDQLKEFGAYNDHWVVARNEEEAEEEAKLIYAGKKFHLTQDPDVL 612
Query: 631 DTWFSSGLFPLSVLGWPDNTEDLKAFYPTSALETGHDILFFWVARMVMLGITLGGDVPFR 690
DTWFSSGLFPLSVLGWPDNTEDLKAFYPTSALETGHDILFFWVARMVMLGITLGGDVPFR
Sbjct: 613 DTWFSSGLFPLSVLGWPDNTEDLKAFYPTSALETGHDILFFWVARMVMLGITLGGDVPFR 672
Query: 691 TIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGNLDPKELAVAKEGQV 750
TIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGNLDPKELAVAKEGQV
Sbjct: 673 TIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGNLDPKELAVAKEGQV 732
Query: 751 KDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAIRFAMSKLGND 810
KDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAIRFAMSKLGND
Sbjct: 733 KDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAIRFAMSKLGND 792
Query: 811 YVPSKNVTPDALPFSCQWILSVLNKAISRTVSSLEAYEFSDATTAVYSWWQYQLCDVFIE 870
YVPSKNVTPDALPFSCQWILSVLNKAISRTVSSLEAYEFSDATTAVYSWWQYQLCDVFIE
Sbjct: 793 YVPSKNVTPDALPFSCQWILSVLNKAISRTVSSLEAYEFSDATTAVYSWWQYQLCDVFIE 852
Query: 871 AIKPYFSANDTEYASARSHAQDTLWLCLENGLRLLHPFMPYVTEELWQRLPYPKSSTRPE 930
AIKPYFS+NDTEYASARSHAQDTLWLCLENGLRLLHPFMPYVTEELWQRLPYPKSSTRPE
Sbjct: 853 AIKPYFSSNDTEYASARSHAQDTLWLCLENGLRLLHPFMPYVTEELWQRLPYPKSSTRPE 912
Query: 931 SIMICDYPSVTEEWTNESVETEMDLIVSAVKSLRSLAKESRERRPGYVLPRNDVVAETIN 990
SIMICDYPSVTEEWTNESVETEMDLIVSAVKSLRSLAKESRERRPGYVLPRNDV+AETIN
Sbjct: 913 SIMICDYPSVTEEWTNESVETEMDLIVSAVKSLRSLAKESRERRPGYVLPRNDVIAETIN 972
Query: 991 KRKLEIVTLANLSSLTVINDNDAAPVGCAVSVVNENLSVYLQFQGAISAEAELEKIKKKM 1050
KRKLEIVTLANLSSLTVINDNDAAPVGCAVSVVNENLSVYLQFQGAISAEAELEKIKKKM
Sbjct: 973 KRKLEIVTLANLSSLTVINDNDAAPVGCAVSVVNENLSVYLQFQGAISAEAELEKIKKKM 1032
Query: 1051 EEVRKQQEKLKKMMDASGYKEKVRPHIHEENVNKLASLMQELLSLEEAGQHIQAHR 1107
EEVRKQQEKLKKMMDASGYKEKVRPHIHEENVNKLASLMQELLSLEEAGQHIQAHR
Sbjct: 1033 EEVRKQQEKLKKMMDASGYKEKVRPHIHEENVNKLASLMQELLSLEEAGQHIQAHR 1088
BLAST of Carg08430 vs. ExPASy Swiss-Prot
Match:
P93736 (Valine--tRNA ligase, mitochondrial 1 OS=Arabidopsis thaliana OX=3702 GN=TWN2 PE=1 SV=2)
HSP 1 Score: 1606.7 bits (4159), Expect = 0.0e+00
Identity = 774/1054 (73.43%), Postives = 895/1054 (84.91%), Query Frame = 0
Query: 63 ESVKKPEVEDP-EKKKKKEEKAREKELKKKKALEK----ALKL-QAQQTSNAPKKSEKKN 122
ES KK E+ E+KKKKEEKA+EKELKK+KALEK LK QA+ +N PKKS KK+
Sbjct: 47 ESEKKILTEEELERKKKKEEKAKEKELKKQKALEKERLAELKAKQAKDGTNVPKKSAKKS 106
Query: 123 ARRGGDEENAEDFVDPDTPFGNKKLMSQQMSKQYNPSAVEKSWYEWWEKSGFFVADAKSS 182
++R EEN EDFVDP+TP G +K +S QM+KQY+P+ VEKSWY WWEKS F ADAKSS
Sbjct: 107 SKRDASEENPEDFVDPETPLGERKRLSSQMAKQYSPATVEKSWYAWWEKSDLFKADAKSS 166
Query: 183 KPPFVIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVE 242
KPPFVIVLPPPNVTGALHIGHALT+AIED IIRW+RMSGYN LWVPG DHAGIATQVVVE
Sbjct: 167 KPPFVIVLPPPNVTGALHIGHALTSAIEDTIIRWKRMSGYNALWVPGVDHAGIATQVVVE 226
Query: 243 KKIMRERNLTRHDIGREKFISEVWDWKTKYGGTILKQLRRLGASLDWTRECFTMDEKRSR 302
KKIMR+R +TRHD+GRE+F+ EVW WK +YGGTIL QLRRLGASLDW+RECFTMDE+RS+
Sbjct: 227 KKIMRDRGMTRHDVGREEFVKEVWKWKNQYGGTILTQLRRLGASLDWSRECFTMDEQRSK 286
Query: 303 AVTEAFVRLFKNGLIYRDLRLVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYEKPVEFG 362
AVTEAFVRL+K GLIYRD+RLVNWDC+LRTAISD+EV+YIDIKEKTLLKVPGYEKPVEFG
Sbjct: 287 AVTEAFVRLYKEGLIYRDIRLVNWDCILRTAISDVEVEYIDIKEKTLLKVPGYEKPVEFG 346
Query: 363 VLTSFAYPLEGELGEIVVATTRVETMLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPI 422
+LTSFAYPLEG LGE++VATTRVETMLGDTAIAIHPDD RYKHLHGKFA+HPFNGRKLPI
Sbjct: 347 LLTSFAYPLEGGLGEVIVATTRVETMLGDTAIAIHPDDARYKHLHGKFAVHPFNGRKLPI 406
Query: 423 ICDAILVDPNFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFEGMPR 482
ICD ILVDPNFGTG VKITPAHDPND EVGKRH LEFINIFTDDGKIN+NGGS+F GMPR
Sbjct: 407 ICDGILVDPNFGTGCVKITPAHDPNDCEVGKRHKLEFINIFTDDGKINTNGGSDFAGMPR 466
Query: 483 FKVREMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAV 542
F RE VV+ALQK+GLYRGAK+NEMRLGLCSR+NDV+EPMIKPQWYVNC + K++LD
Sbjct: 467 FAAREAVVEALQKQGLYRGAKNNEMRLGLCSRTNDVIEPMIKPQWYVNCSMIGKEALDVA 526
Query: 543 MDNECKKIEIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATLDDDQLKEFGAYN 602
+ +E KK+E +PKQY+A+W+RWLENIRDWCISRQLWWGHRIPAWYATL++DQLKE GAY+
Sbjct: 527 ITDENKKLEFVPKQYTAEWRRWLENIRDWCISRQLWWGHRIPAWYATLEEDQLKEVGAYS 586
Query: 603 DHWVVARNEEEAEEEAKLIYAGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPDNTEDLKAF 662
DHWVVAR E++A EEA + GKKF LT+DPDVLDTWFSSGLFPLSVLGWPD T+D KAF
Sbjct: 587 DHWVVARTEDDAREEAAQKFLGKKFELTRDPDVLDTWFSSGLFPLSVLGWPDVTDDFKAF 646
Query: 663 YPTSALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDP 722
YPTS LETGHDILFFWVARMVM+G+ LGG+VPF +Y HPMIRDAHGRKMSKSLGNVIDP
Sbjct: 647 YPTSVLETGHDILFFWVARMVMMGMKLGGEVPFSKVYFHPMIRDAHGRKMSKSLGNVIDP 706
Query: 723 VEVINGISLEGLHKRLEEGNLDPKELAVAKEGQVKDFPNGISECGADALRFALISYTAQS 782
+EVING++LEGLHKRLEEGNLDPKE+ VAKEGQVKDFPNGI ECG DALRFAL+SYTAQS
Sbjct: 707 LEVINGVTLEGLHKRLEEGNLDPKEVIVAKEGQVKDFPNGIPECGTDALRFALVSYTAQS 766
Query: 783 DKINLDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVPSKNVTPDALPFSCQWILSVLNKA 842
DKINLDI RVVGYRQWCNKLWNA+RFAM KLG+ Y P + ++P+ +PFSCQWILSVLNKA
Sbjct: 767 DKINLDILRVVGYRQWCNKLWNAVRFAMMKLGDGYTPPQTLSPETMPFSCQWILSVLNKA 826
Query: 843 ISRTVSSLEAYEFSDATTAVYSWWQYQLCDVFIEAIKPYFSANDTEYASARSHAQDTLWL 902
IS+TV SL+A+EFSDA +Y+WWQYQ CDV+IEAIKPYF+ ++ +AS R+HAQ LW+
Sbjct: 827 ISKTVVSLDAFEFSDAANTIYAWWQYQFCDVYIEAIKPYFAGDNPTFASERAHAQHALWI 886
Query: 903 CLENGLRLLHPFMPYVTEELWQRLPYPKSSTRPESIMICDYPSVTEEWTNESVETEMDLI 962
LE GLRLLHPFMP+VTEELWQRLP PK + R SIMICDYPS E W+NE VE+EMD +
Sbjct: 887 SLETGLRLLHPFMPFVTEELWQRLPAPKDTERKASIMICDYPSAIENWSNEKVESEMDTV 946
Query: 963 VSAVKSLRS-----LAKESRERRPGYVLPRNDVVAETINKRKLEIVTLANLSSLTVINDN 1022
++ VK +R+ L K+ ER P + L N+V +E + +LEI TLANLSSL V++
Sbjct: 947 LATVKCMRALRAGLLEKQKNERLPAFALCENNVTSEIVKSHELEIRTLANLSSLEVVSKG 1006
Query: 1023 D-AAPVGCAVSVVNENLSVYLQFQGAISAEAELEKIKKKMEEVRKQQEKLKKMMDASGYK 1082
AAP G +V VNENL VYL+ GAI+ EAE EKI+ K+ E++KQ+EKL+KMM S Y+
Sbjct: 1007 QHAAPPGSSVETVNENLKVYLEVDGAINTEAEQEKIRNKIGELQKQKEKLQKMMSVSTYE 1066
Query: 1083 EKVRPHIHEENVNKLASLMQELLSLEEAGQHIQA 1105
EKV +I E+N NKLA ++QE E+ + A
Sbjct: 1067 EKVPANIKEDNANKLAKILQEFDFFEKESARLAA 1100
BLAST of Carg08430 vs. ExPASy Swiss-Prot
Match:
Q86KU2 (Probable valine--tRNA ligase, cytoplasmic OS=Dictyostelium discoideum OX=44689 GN=valS1 PE=3 SV=1)
HSP 1 Score: 1036.2 bits (2678), Expect = 2.7e-301
Identity = 535/1051 (50.90%), Postives = 705/1051 (67.08%), Query Frame = 0
Query: 60 KDMESVKKPEVEDPEKKKKKEEKAREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARR 119
K+ E K E E EK K +EK ++ K+K L+ LK + ++ ++ EK+ A
Sbjct: 20 KNEEKKKAKEAEKAEKLAKMKEKEAKQAAAKEKELQNKLKKEQEKKEKEAEEKEKQEAEE 79
Query: 120 GGDEENAEDFVDPDTPFGNKKLMSQQMSKQYNPSAVEKSWYEWWEKSGFFVADAKSSKPP 179
E+ E + TP G KK +S +S Y+P+AVE WY++W +G+F + + P
Sbjct: 80 KIVREHIEKIL--KTPKGEKKDVSSLLS-SYHPTAVESIWYDYWLDNGYFSPEKQMEIQP 139
Query: 180 -------FVIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQ 239
FVIV+PPPNVTG+LH+GHALT +I+DA++R+ RM G LWVPGTDHAGIATQ
Sbjct: 140 HVVKDKKFVIVIPPPNVTGSLHLGHALTNSIQDAVVRYHRMKGEVCLWVPGTDHAGIATQ 199
Query: 240 VVVEKKIMRERNLTRHDIGREKFISEVWDWKTKYGGTILKQLRRLGASLDWTRECFTMDE 299
VVVEKKI +E +TRHD+GRE FI +VW+WK +YG I QL+++G+S+DW+RE FTMD+
Sbjct: 200 VVVEKKIWKENKITRHDLGREGFIKKVWEWKGEYGARIQGQLKKMGSSVDWSREVFTMDD 259
Query: 300 KRSRAVTEAFVRLFKNGLIYRDLRLVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYEKP 359
+RS+AV AF+R+F +GLI R RLVNW C L+TAISDIEVD+ID+++ T + VPG++
Sbjct: 260 QRSKAVNTAFIRMFNDGLIVRTTRLVNWSCALKTAISDIEVDFIDLEKHTKMSVPGHQGQ 319
Query: 360 VEFGVLTSFAYPLEGELGEIVVATTRVETMLGDTAIAIHPDDTRYKHLHGKFAIHPFNGR 419
+FGVL FAY +EG E+VVATTR+ETML DTAIAIHPDDTRY HLHGKFAIHP NGR
Sbjct: 320 YDFGVLFEFAYQVEGSGEELVVATTRIETMLADTAIAIHPDDTRYTHLHGKFAIHPLNGR 379
Query: 420 KLPIICDAILVDPNFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFE 479
K+PII D++LVD +FGTG VKITP+HDPND+E RH LEFIN+FTD+G IN NGG F
Sbjct: 380 KIPIITDSVLVDKDFGTGVVKITPSHDPNDYETALRHGLEFINLFTDEGLINENGGERFA 439
Query: 480 GMPRFKVREMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDMAKQS 539
GM RF R VV+AL++K L++G KDN+MRLGLCSRS DV+EPMIKPQWYV C DMA ++
Sbjct: 440 GMKRFDARNAVVEALKEKNLFKGMKDNKMRLGLCSRSKDVIEPMIKPQWYVKCDDMAARA 499
Query: 540 LDAVMDNECKKIEIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATLDDDQLKEF 599
+ AV D + K I PK + W RWLE I+DWC+SRQLWWGHRIPA++ + + +
Sbjct: 500 VKAVRDGDLK---ITPKTHEVTWYRWLEGIKDWCVSRQLWWGHRIPAYHCIIKGVKSNPY 559
Query: 600 GAYNDHWVVARNEEEAEEEA----KLIYAGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPD 659
+ WVV N+EEA + A KL L QDPDVLDTWFSSGLFP SV+GWP+
Sbjct: 560 D--TNQWVVGANQEEATQNAIEKFKLSSKDDILSLEQDPDVLDTWFSSGLFPFSVMGWPE 619
Query: 660 NTEDLKAFYPTSALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSK 719
T+D++ +YPTS LETG DILFFWVARMVM+G L G +PF ++LH M+RD+HGRKMSK
Sbjct: 620 QTQDMQDYYPTSLLETGSDILFFWVARMVMMGQQLTGKLPFEQVFLHAMVRDSHGRKMSK 679
Query: 720 SLGNVIDPVEVINGISLEGLHKRLEEGNLDPKELAVAKEGQVKDFPNGISECGADALRFA 779
SLGNVIDP +VI GISL+ L +L EGNLD KE+ A G DFP GI+ECG DA+RFA
Sbjct: 680 SLGNVIDPNDVIKGISLDELIAKLYEGNLDSKEIEKATSGVKSDFPTGIAECGTDAMRFA 739
Query: 780 LISYTAQSDKINLDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVPSKNVTPDALPFSCQ- 839
L +YT+Q INLDIQRVVGYR +CNK+WNA RFA KLG+ + P L S
Sbjct: 740 LCAYTSQGRDINLDIQRVVGYRNFCNKIWNATRFAHMKLGDSFKPESFDASKLLQQSNAI 799
Query: 840 --WILSVLNKAISRTVSSLEAYEFSDATTAVYSWWQYQLCDVFIEAIKPYFSANDTEYAS 899
WIL+ +AI+ + + Y+FS TTA+YS+W ++CDV++E K FS E +
Sbjct: 800 NLWILNAAQRAITLSEEGFKEYDFSKVTTAIYSFWLNEMCDVYLEMTKSIFSLE--EDSP 859
Query: 900 ARSHAQDTLWLCLENGLRLLHPFMPYVTEELWQRLPYPKSSTRPESIMICDYPSVTEEWT 959
+ ++TL+ C++ GLRLLHPFMPY+TEEL+Q LP P +IM+C YP W
Sbjct: 860 IKQKTKETLYTCIDIGLRLLHPFMPYLTEELYQSLPRRPEDVIP-TIMLCPYPQSQSCWA 919
Query: 960 NESVETEMDLIVSAVKSLRSLAK----ESRERRPGYVLPRNDVVAETINKRKLEIVTLAN 1019
N +E EM +KS+RSL + ++ Y+ +N E K I LA
Sbjct: 920 NSDIEEEMKQCQDIIKSIRSLRATYTIPASKKLHTYLHVKNQENLERFTKNSSFIRVLAY 979
Query: 1020 LSSLTVINDNDAAPVGCAVSVVNENLSVYLQFQGAISAEAELEKIKKKMEEVRKQQEKLK 1079
S L V +++ P GC V+VVNEN+S L +G++ E+ +++ K +++ K E L
Sbjct: 980 ASELEVSISDESRP-GCIVNVVNENVSTLLDVRGSVDFNLEIARLETKKQQLVKNYETLV 1039
Query: 1080 KMMDASGYKEKVRPHIHEENVNKLASLMQEL 1093
Y +KV +I ++N K+ +L +E+
Sbjct: 1040 SKTTIPSY-DKVPQNIRDDNTQKIKALDEEI 1057
BLAST of Carg08430 vs. ExPASy Swiss-Prot
Match:
Q9Z1Q9 (Valine--tRNA ligase OS=Mus musculus OX=10090 GN=Vars1 PE=1 SV=1)
HSP 1 Score: 974.2 bits (2517), Expect = 1.3e-282
Identity = 510/1033 (49.37%), Postives = 677/1033 (65.54%), Query Frame = 0
Query: 85 EKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRGGDEENAEDFV-DPDTPFGNKKLMS 144
+KE KK++ LEK Q Q+T P EKK ++ + D TP G KK +S
Sbjct: 235 KKEAKKREKLEKF--QQKQKTQQQPPHGEKKPKPEKKEKRDPGVITYDLPTPPGEKKDVS 294
Query: 145 QQMSKQYNPSAVEKSWYEWWEKSGFFVAD-------AKSSKPPFVIVLPPPNVTGALHIG 204
M Y+P VE +WY WWE+ GFF + A + + F++ +PPPNVTG+LH+G
Sbjct: 295 GAMPDSYSPQYVEAAWYPWWERQGFFKPEYGRPSVSAPNPRGVFMMCIPPPNVTGSLHLG 354
Query: 205 HALTAAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKFI 264
HALT AI+D++ RW RM G TLW PG DHAGIATQVVVEKK+ +ER L RH +GRE F+
Sbjct: 355 HALTNAIQDSLTRWHRMRGETTLWNPGCDHAGIATQVVVEKKLWKERGLNRHQLGREAFL 414
Query: 265 SEVWDWKTKYGGTILKQLRRLGASLDWTRECFTMDEKRSRAVTEAFVRLFKNGLIYRDLR 324
EVW WK + G I QL++LG+SLDW R CFTMD K S VTEAFVRL + G+IYR R
Sbjct: 415 EEVWKWKAEKGDRIYHQLKKLGSSLDWDRACFTMDPKLSATVTEAFVRLHEEGVIYRSTR 474
Query: 325 LVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYEKPVEFGVLTSFAYPLEGELG--EIVV 384
LVNW C L +AISDIEVD ++ +TLL VPGY++ VEFGVL SFAY ++G E+VV
Sbjct: 475 LVNWSCTLNSAISDIEVDKKELTGRTLLPVPGYKEKVEFGVLVSFAYKVQGSDSDEEVVV 534
Query: 385 ATTRVETMLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPIICDAILVDPNFGTGAVKI 444
ATTR+ETMLGD A+A+HP D RY+HL GK +HPF R LPI+ D VD FGTGAVKI
Sbjct: 535 ATTRIETMLGDVAVAVHPKDPRYQHLKGKCVVHPFLSRSLPIVFDD-FVDMEFGTGAVKI 594
Query: 445 TPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFEGMPRFKVREMVVDALQKKGLYR 504
TPAHD ND+EVG+RH LE I+I G + N F G+PRF+ R+ V+ AL+++GL+R
Sbjct: 595 TPAHDQNDYEVGQRHRLEAISIMDSKGAL-INVPPPFLGLPRFEARKAVLAALKERGLFR 654
Query: 505 GAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAVMDNECKKIEIIPKQYSAD 564
G KDN M + LC+RS DVVEP+++PQWYV C +MA+ + AV + + I+P+ +
Sbjct: 655 GVKDNPMVVPLCNRSKDVVEPLLRPQWYVRCGEMAQAASAAVTRGD---LRILPEAHQRT 714
Query: 565 WKRWLENIRDWCISRQLWWGHRIPAWYATLDDDQLKEFGAYND--HWVVARNEEEAEEEA 624
W W++NIRDWCISRQLWWGHRIPA++ T+ D + G D +WV R E EA E+A
Sbjct: 715 WHSWMDNIRDWCISRQLWWGHRIPAYFITVHDPAVPP-GEDPDGRYWVSGRTEAEAREKA 774
Query: 625 KLIY--AGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPDNTEDLKAFYPTSALETGHDILF 684
+ + K L QD DVLDTWFSSGLFP S+ GWP+ +EDL FYP + LETGHDILF
Sbjct: 775 AREFGVSPDKISLQQDEDVLDTWFSSGLFPFSIFGWPNQSEDLSVFYPGTLLETGHDILF 834
Query: 685 FWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHK 744
FWVARMVMLG+ L G +PFR +YLH ++RDAHGRKMSKSLGNVIDP++VI+G+SL+GL+
Sbjct: 835 FWVARMVMLGLKLTGKLPFREVYLHAIVRDAHGRKMSKSLGNVIDPLDVIHGVSLQGLYD 894
Query: 745 RLEEGNLDPKELAVAKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYR 804
+L NLDP E+ AKEGQ DFP GI ECG DALRF L +YT+Q INLD+ R++GYR
Sbjct: 895 QLLNSNLDPSEVEKAKEGQKADFPAGIPECGTDALRFGLCAYTSQGRDINLDVNRILGYR 954
Query: 805 QWCNKLWNAIRFAMSKLGNDYVPSKNVTPDA-LPFSCQWILSVLNKAISRTVSSLEAYEF 864
+CNKLWNA +FA+ LG +VPS P+ +WI S L +A+ + +AY+F
Sbjct: 955 HFCNKLWNATKFALRGLGKGFVPSATSKPEGHESLVDRWIRSRLTEAVRLSNEGFQAYDF 1014
Query: 865 SDATTAVYSWWQYQLCDVFIEAIKPYFSANDTEYASARSHAQDTLWLCLENGLRLLHPFM 924
TTA YS+W Y+LCDV++E +KP + D A A+ TL+ CL+ GLRLL PFM
Sbjct: 1015 PAITTAQYSFWLYELCDVYLECLKPVLNGVDQVAAEC---ARQTLYTCLDVGLRLLSPFM 1074
Query: 925 PYVTEELWQRLPYPKSSTRPESIMICDYPSVTE-EWTNESVETEMDLIVSAVKSLRSLAK 984
P+VTEEL+QRLP ++ P S+ + YP +E W + E ++L +S +++RSL
Sbjct: 1075 PFVTEELFQRLP-RRTPKAPASLCVTPYPEPSECSWKDPEAEAALELALSITRAVRSLRA 1134
Query: 985 E---SRERRPGYVLPRNDVVAETINKRKLEIVTLANLSSLTVINDNDAAPVGCAVSVVNE 1044
+ +R R ++ ++ + + LA+ + V+ AP GCAV+V ++
Sbjct: 1135 DYNLTRTRPDCFLEVADEATGALASAVSGYVQALASAGVVAVLALGAPAPQGCAVAVASD 1194
Query: 1045 NLSVYLQFQGAISAEAELEKIKKKMEEVRKQQEKLKKMMDASGYKEKVRPHIHEENVNKL 1099
S++LQ QG + EL K++ K E ++Q ++L++ AS Y KV + E + KL
Sbjct: 1195 RCSIHLQLQGLVDPARELGKLQAKRSEAQRQAQRLQERRAASSYSAKVPLEVQEADEAKL 1254
BLAST of Carg08430 vs. ExPASy Swiss-Prot
Match:
P26640 (Valine--tRNA ligase OS=Homo sapiens OX=9606 GN=VARS1 PE=1 SV=4)
HSP 1 Score: 973.8 bits (2516), Expect = 1.7e-282
Identity = 521/1082 (48.15%), Postives = 692/1082 (63.96%), Query Frame = 0
Query: 35 LSCVHRAPFSSFSKGICPEFSVAPLKDMESVKKPEVEDPEKKKKKEEKAREKELKKKKAL 94
++CV + F + + PL + P + + KKE K REK L
Sbjct: 192 VTCVRQPEFRAVLGEVVLYSGARPLSHQPGPEAPALPKTAAQLKKEAKKREK-------L 251
Query: 95 EKALKLQAQQTSNAPKKSEKKNARRGGDEENAEDFVDPDTPFGNKKLMSQQMSKQYNPSA 154
EK + Q Q P +K + + D TP G KK +S M Y+P
Sbjct: 252 EKFQQKQKIQQQQPPPGEKKPKPEKREKRDPGVITYDLPTPPGEKKDVSGPMPDSYSPRY 311
Query: 155 VEKSWYEWWEKSGFFVAD-------AKSSKPPFVIVLPPPNVTGALHIGHALTAAIEDAI 214
VE +WY WWE+ GFF + A + + F++ +PPPNVTG+LH+GHALT AI+D++
Sbjct: 312 VEAAWYPWWEQQGFFKPEYGRPNVSAANPRGVFMMCIPPPNVTGSLHLGHALTNAIQDSL 371
Query: 215 IRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKFISEVWDWKTKYG 274
RW RM G TLW PG DHAGIATQVVVEKK+ RE+ L+RH +GRE F+ EVW WK + G
Sbjct: 372 TRWHRMRGETTLWNPGCDHAGIATQVVVEKKLWREQGLSRHQLGREAFLQEVWKWKEEKG 431
Query: 275 GTILKQLRRLGASLDWTRECFTMDEKRSRAVTEAFVRLFKNGLIYRDLRLVNWDCVLRTA 334
I QL++LG+SLDW R CFTMD K S AVTEAFVRL + G+IYR RLVNW C L +A
Sbjct: 432 DRIYHQLKKLGSSLDWDRACFTMDPKLSAAVTEAFVRLHEEGIIYRSTRLVNWSCTLNSA 491
Query: 335 ISDIEVDYIDIKEKTLLKVPGYEKPVEFGVLTSFAYPLEGELG--EIVVATTRVETMLGD 394
ISDIEVD ++ +TLL VPGY++ VEFGVL SFAY ++G E+VVATTR+ETMLGD
Sbjct: 492 ISDIEVDKKELTGRTLLSVPGYKEKVEFGVLVSFAYKVQGSDSDEEVVVATTRIETMLGD 551
Query: 395 TAIAIHPDDTRYKHLHGKFAIHPFNGRKLPIICDAILVDPNFGTGAVKITPAHDPNDFEV 454
A+A+HP DTRY+HL GK IHPF R LPI+ D VD +FGTGAVKITPAHD ND+EV
Sbjct: 552 VAVAVHPKDTRYQHLKGKNVIHPFLSRSLPIVFDE-FVDMDFGTGAVKITPAHDQNDYEV 611
Query: 455 GKRHNLEFINIFTDDGKINSNGGSEFEGMPRFKVREMVVDALQKKGLYRGAKDNEMRLGL 514
G+RH LE I+I G + N F G+PRF+ R+ V+ AL+++GL+RG +DN M + L
Sbjct: 612 GQRHGLEAISIMDSRGAL-INVPPPFLGLPRFEARKAVLVALKERGLFRGIEDNPMVVPL 671
Query: 515 CSRSNDVVEPMIKPQWYVNCKDMAKQSLDAVMDNECKKIEIIPKQYSADWKRWLENIRDW 574
C+RS DVVEP+++PQWYV C +MA+ + AV + + I+P+ + W W++NIR+W
Sbjct: 672 CNRSKDVVEPLLRPQWYVRCGEMAQAASAAVTRGD---LRILPEAHQRTWHAWMDNIREW 731
Query: 575 CISRQLWWGHRIPAWYATLDDDQLKEFGAYND--HWVVARNEEEAEEEAKLIY--AGKKF 634
CISRQLWWGHRIPA++ T+ D + G D +WV RNE EA E+A + + K
Sbjct: 732 CISRQLWWGHRIPAYFVTVSDPAVPP-GEDPDGRYWVSGRNEAEAREKAAKEFGVSPDKI 791
Query: 635 HLTQDPDVLDTWFSSGLFPLSVLGWPDNTEDLKAFYPTSALETGHDILFFWVARMVMLGI 694
L QD DVLDTWFSSGLFPLS+LGWP+ +EDL FYP + LETGHDILFFWVARMVMLG+
Sbjct: 792 SLQQDEDVLDTWFSSGLFPLSILGWPNQSEDLSVFYPGTLLETGHDILFFWVARMVMLGL 851
Query: 695 TLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGNLDPKE 754
L G +PFR +YLH ++RDAHGRKMSKSLGNVIDP++VI GISL+GLH +L NLDP E
Sbjct: 852 KLTGRLPFREVYLHAIVRDAHGRKMSKSLGNVIDPLDVIYGISLQGLHNQLLNSNLDPSE 911
Query: 755 LAVAKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAIR 814
+ AKEGQ DFP GI ECG DALRF L +Y +Q INLD+ R++GYR +CNKLWNA +
Sbjct: 912 VEKAKEGQKADFPAGIPECGTDALRFGLCAYMSQGRDINLDVNRILGYRHFCNKLWNATK 971
Query: 815 FAMSKLGNDYVPSKNVTPDA-LPFSCQWILSVLNKAISRTVSSLEAYEFSDATTAVYSWW 874
FA+ LG +VPS P +WI S L +A+ + +AY+F TTA YS+W
Sbjct: 972 FALRGLGKGFVPSPTSQPGGHESLVDRWIRSRLTEAVRLSNQGFQAYDFPAVTTAQYSFW 1031
Query: 875 QYQLCDVFIEAIKPYFSANDTEYASARSHAQDTLWLCLENGLRLLHPFMPYVTEELWQRL 934
Y+LCDV++E +KP + D A A+ TL+ CL+ GLRLL PFMP+VTEEL+QRL
Sbjct: 1032 LYELCDVYLECLKPVLNGVDQVAAEC---ARQTLYTCLDVGLRLLSPFMPFVTEELFQRL 1091
Query: 935 PYPKSSTRPESIMICDYPSVTE-EWTNESVETEMDLIVSAVKSLRSLAKE---SRERRPG 994
P + P S+ + YP +E W + E ++L +S +++RSL + +R R
Sbjct: 1092 P-RRMPQAPPSLCVTPYPEPSECSWKDPEAEAALELALSITRAVRSLRADYNLTRIRPDC 1151
Query: 995 YVLPRNDVVAETINKRKLEIVTLANLSSLTVINDNDAAPVGCAVSVVNENLSVYLQFQGA 1054
++ ++ + + LA+ + V+ AP GCAV++ ++ S++LQ QG
Sbjct: 1152 FLEVADEATGALASAVSGYVQALASAGVVAVLALGAPAPQGCAVALASDRCSIHLQLQGL 1211
Query: 1055 ISAEAELEKIKKKMEEVRKQQEKLKKMMDASGYKEKVRPHIHEENVNKLASLMQELLSLE 1099
+ EL K++ K E ++Q ++L++ ASGY KV + E + KL EL ++
Sbjct: 1212 VDPARELGKLQAKRVEAQRQAQRLRERRAASGYPVKVPLEVQEADEAKLQQTEAELRKVD 1256
BLAST of Carg08430 vs. ExPASy Swiss-Prot
Match:
Q04462 (Valine--tRNA ligase OS=Rattus norvegicus OX=10116 GN=Vars1 PE=2 SV=2)
HSP 1 Score: 973.0 bits (2514), Expect = 2.8e-282
Identity = 521/1053 (49.48%), Postives = 688/1053 (65.34%), Query Frame = 0
Query: 69 EVEDPEK---KKKKEEKAREKELKKKKALEKALKLQAQQTSNAPK--KSEKKNARRGGDE 128
E+ P+K + KKE K REK L+K ++ K Q QQ ++ K K EKK R G
Sbjct: 223 EITAPQKTAAQLKKEAKKREK-LEK---FQQKQKTQQQQPAHGEKKPKPEKKEKRDPG-- 282
Query: 129 ENAEDFVDPDTPFGNKKLMSQQMSKQYNPSAVEKSWYEWWEKSGFFVAD-------AKSS 188
D TP G KK +S M Y+P VE +WY WWE+ GFF + A +
Sbjct: 283 ---VITYDLPTPPGEKKDVSGTMPDSYSPQYVEAAWYPWWERQGFFKPEYGRPSVSAPNP 342
Query: 189 KPPFVIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVE 248
+ F++ +PPPNVTG+LH+GHALT AI+D++ RW RM G TLW PG DHAGIATQVVVE
Sbjct: 343 RGVFMMCIPPPNVTGSLHLGHALTNAIQDSLTRWHRMRGETTLWNPGCDHAGIATQVVVE 402
Query: 249 KKIMRERNLTRHDIGREKFISEVWDWKTKYGGTILKQLRRLGASLDWTRECFTMDEKRSR 308
KK+ +ER L RH +GRE F+ EVW WK + G I QL++LG+SLDW R CFTMD K S
Sbjct: 403 KKLWKERGLNRHQLGREAFLQEVWKWKAEKGDRIYHQLKKLGSSLDWDRACFTMDPKLSA 462
Query: 309 AVTEAFVRLFKNGLIYRDLRLVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYEKPVEFG 368
VTEAFVRL + G+IYR RLVNW C L +AISDIEVD ++ +TLL VPGY++ VEFG
Sbjct: 463 TVTEAFVRLHEEGVIYRSTRLVNWSCTLNSAISDIEVDKKELTGRTLLSVPGYKEKVEFG 522
Query: 369 VLTSFAYPLEGELG--EIVVATTRVETMLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKL 428
VL SFAY ++G E+VVATTR+ETMLGD A+A+HP D RY+HL GK +HPF R L
Sbjct: 523 VLVSFAYKVQGSDSDEEVVVATTRIETMLGDVAVAVHPKDPRYQHLKGKSVVHPFLSRSL 582
Query: 429 PIICDAILVDPNFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFEGM 488
PI+ D VD FGTGAVKITPAHD ND+EVG+RH LE I+I G + N F G+
Sbjct: 583 PIVFDD-FVDMEFGTGAVKITPAHDQNDYEVGQRHRLEAISIMDSKGAL-VNVPPPFLGL 642
Query: 489 PRFKVREMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLD 548
PRF+ R+ V+ AL+++GL+RG KDN M + LC+RS DVVEP+++PQWYV C +MA+ +
Sbjct: 643 PRFEARKAVLAALKEQGLFRGIKDNPMVVPLCNRSKDVVEPLLRPQWYVRCGEMAQAASA 702
Query: 549 AVMDNECKKIEIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATLDDDQLKEFGA 608
AV + + I+P+ + W W++NIRDWCISRQLWWGHRIPA++ T+ D + G
Sbjct: 703 AVTRGD---LRILPEAHQRTWHSWMDNIRDWCISRQLWWGHRIPAYFITVHDPAVPP-GE 762
Query: 609 YND--HWVVARNEEEAEEEAKLIY--AGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPDNT 668
D +WV R E EA E+A + + K L QD DVLDTWFSSGLFP S+ GWP+ +
Sbjct: 763 DPDGRYWVSGRTEAEAREKAAREFGVSPDKISLQQDEDVLDTWFSSGLFPFSIFGWPNQS 822
Query: 669 EDLKAFYPTSALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSL 728
EDL FYP + LETGHDILFFWVARMVMLG+ L +PFR +YLH ++RDAHGRKMSKSL
Sbjct: 823 EDLSVFYPGTLLETGHDILFFWVARMVMLGLKLTEKLPFREVYLHAIVRDAHGRKMSKSL 882
Query: 729 GNVIDPVEVINGISLEGLHKRLEEGNLDPKELAVAKEGQVKDFPNGISECGADALRFALI 788
GNVIDP++VI+G+SL+GLH +L NLDP E+ AKEGQ DFP GI ECG DALRF L
Sbjct: 883 GNVIDPLDVIHGVSLQGLHDQLLNSNLDPSEVEKAKEGQRADFPAGIPECGTDALRFGLC 942
Query: 789 SYTAQSDKINLDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVPSKNVTPDA-LPFSCQWI 848
+YT+Q INLD+ R++GYR +CNKLWNA +FA+ LG +VPS P+ +WI
Sbjct: 943 AYTSQGRDINLDVNRILGYRHFCNKLWNATKFALRGLGKGFVPSPTSKPEGHESLVDRWI 1002
Query: 849 LSVLNKAISRTVSSLEAYEFSDATTAVYSWWQYQLCDVFIEAIKPYFSANDTEYASARSH 908
S L +A+ + +AY+F TTA YS+W Y+LCDV++E +KP + D A
Sbjct: 1003 RSRLAEAVRLSNEGFQAYDFPAVTTAQYSFWLYELCDVYLECLKPVLNGVDQVAADC--- 1062
Query: 909 AQDTLWLCLENGLRLLHPFMPYVTEELWQRLPYPKSSTRPESIMICDYPSVTE-EWTNES 968
A+ TL+ CL+ GLRLL PFMP+VTEEL+QRLP ++ P S+ + YP +E W +
Sbjct: 1063 ARQTLYTCLDVGLRLLSPFMPFVTEELFQRLP-RRTPNAPASLCVTPYPEPSECSWKDPE 1122
Query: 969 VETEMDLIVSAVKSLRSLAKE---SRERRPGYVLPRNDVVAETINKRKLEIVTLANLSSL 1028
E ++L +S +++RSL + +R R ++ ++ + + TLA+ +
Sbjct: 1123 AEAALELALSITRAVRSLRADYNLTRTRPDCFLEVADEATGALASAVSAYVQTLASAGVV 1182
Query: 1029 TVINDNDAAPVGCAVSVVNENLSVYLQFQGAISAEAELEKIKKKMEEVRKQQEKLKKMMD 1088
V+ AP GCAV+V ++ S++LQ QG + EL K++ K E ++Q ++L++
Sbjct: 1183 AVLALGAPAPQGCAVAVASDRCSIHLQLQGLVDPARELGKLQAKRSEAQRQAQRLQERRA 1242
Query: 1089 ASGYKEKVRPHIHEENVNKLASLMQELLSLEEA 1099
ASGY KV + E + KL EL ++EA
Sbjct: 1243 ASGYSAKVPLEVQEADEVKLQQTEAELRKVDEA 1256
BLAST of Carg08430 vs. ExPASy TrEMBL
Match:
A0A6J1H3K8 (Valyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111459332 PE=3 SV=1)
HSP 1 Score: 2220.7 bits (5753), Expect = 0.0e+00
Identity = 1100/1106 (99.46%), Postives = 1103/1106 (99.73%), Query Frame = 0
Query: 1 MRNGILNLLAVCVKQGSVLGEPLLVSLFSRQSYKLSCVHRAPFSSFSKGICPEFSVAPLK 60
MRNGILNLLAVCVKQG VLGEPLLVSLFSRQSYKLSCVHRAPFSSFSKGICPEFSVAPLK
Sbjct: 1 MRNGILNLLAVCVKQGRVLGEPLLVSLFSRQSYKLSCVHRAPFSSFSKGICPEFSVAPLK 60
Query: 61 DMESVKKPEVEDPEKKKKKEEKAREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRG 120
DMESVKKPEVEDPEKKKKKEEKAREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRG
Sbjct: 61 DMESVKKPEVEDPEKKKKKEEKAREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRG 120
Query: 121 GDEENAEDFVDPDTPFGNKKLMSQQMSKQYNPSAVEKSWYEWWEKSGFFVADAKSSKPPF 180
GDEENAEDFVDPDTPFG KKLMSQQMSKQYNPSAVEKSWYEWWEKSGFFVADAKSSKPPF
Sbjct: 121 GDEENAEDFVDPDTPFGKKKLMSQQMSKQYNPSAVEKSWYEWWEKSGFFVADAKSSKPPF 180
Query: 181 VIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIM 240
VIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIM
Sbjct: 181 VIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIM 240
Query: 241 RERNLTRHDIGREKFISEVWDWKTKYGGTILKQLRRLGASLDWTRECFTMDEKRSRAVTE 300
RERNLTRHDIGREKFISEVWDWKTKYGGTILKQLRRLGASLDWTRECFTMDEKRSRAVTE
Sbjct: 241 RERNLTRHDIGREKFISEVWDWKTKYGGTILKQLRRLGASLDWTRECFTMDEKRSRAVTE 300
Query: 301 AFVRLFKNGLIYRDLRLVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYEKPVEFGVLTS 360
AFVRLFKNGLIYRDLRLVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYEKPVEFGVLTS
Sbjct: 301 AFVRLFKNGLIYRDLRLVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYEKPVEFGVLTS 360
Query: 361 FAYPLEGELGEIVVATTRVETMLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPIICDA 420
FAYPLEGELGEIVVATTRVETMLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPIICDA
Sbjct: 361 FAYPLEGELGEIVVATTRVETMLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPIICDA 420
Query: 421 ILVDPNFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFEGMPRFKVR 480
ILVDPNFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFEGMPRF+VR
Sbjct: 421 ILVDPNFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFEGMPRFEVR 480
Query: 481 EMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAVMDNE 540
EMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAV DNE
Sbjct: 481 EMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAVTDNE 540
Query: 541 CKKIEIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATLDDDQLKEFGAYNDHWV 600
CKKIEIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATLDDDQLKEFGAYNDHWV
Sbjct: 541 CKKIEIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATLDDDQLKEFGAYNDHWV 600
Query: 601 VARNEEEAEEEAKLIYAGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPDNTEDLKAFYPTS 660
VARNEEEAEEEAKLIYAGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPDNTEDLKAFYPTS
Sbjct: 601 VARNEEEAEEEAKLIYAGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPDNTEDLKAFYPTS 660
Query: 661 ALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVI 720
ALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVI
Sbjct: 661 ALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVI 720
Query: 721 NGISLEGLHKRLEEGNLDPKELAVAKEGQVKDFPNGISECGADALRFALISYTAQSDKIN 780
NGISLEGLHKRLEEGNLDPKELAVAKEGQVKDFPNGISECGADALRFALISYTAQSDKIN
Sbjct: 721 NGISLEGLHKRLEEGNLDPKELAVAKEGQVKDFPNGISECGADALRFALISYTAQSDKIN 780
Query: 781 LDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVPSKNVTPDALPFSCQWILSVLNKAISRT 840
LDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVPSKNVTPDALPFSCQWILSVLNKAISRT
Sbjct: 781 LDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVPSKNVTPDALPFSCQWILSVLNKAISRT 840
Query: 841 VSSLEAYEFSDATTAVYSWWQYQLCDVFIEAIKPYFSANDTEYASARSHAQDTLWLCLEN 900
VSSLEAYEFSDATTAVYSWWQYQLCDVFIEAIKPYFS+NDTEYASARSHAQDTLWLCLEN
Sbjct: 841 VSSLEAYEFSDATTAVYSWWQYQLCDVFIEAIKPYFSSNDTEYASARSHAQDTLWLCLEN 900
Query: 901 GLRLLHPFMPYVTEELWQRLPYPKSSTRPESIMICDYPSVTEEWTNESVETEMDLIVSAV 960
GLRLLHPFMPYVTEELWQRLPYPKSSTRPESIMICDYPSVTEEWTNESVETEMDLIVSAV
Sbjct: 901 GLRLLHPFMPYVTEELWQRLPYPKSSTRPESIMICDYPSVTEEWTNESVETEMDLIVSAV 960
Query: 961 KSLRSLAKESRERRPGYVLPRNDVVAETINKRKLEIVTLANLSSLTVINDNDAAPVGCAV 1020
KSLRSLAKESRERRPGYVLPRNDVVAETINKRKLEIVTLANLSSLTVINDNDAAPVGCAV
Sbjct: 961 KSLRSLAKESRERRPGYVLPRNDVVAETINKRKLEIVTLANLSSLTVINDNDAAPVGCAV 1020
Query: 1021 SVVNENLSVYLQFQGAISAEAELEKIKKKMEEVRKQQEKLKKMMDASGYKEKVRPHIHEE 1080
SV+NENLSVYLQFQGAISAEAELEKIKKKMEEVRKQQEKLKKMMDASGYKEKVRPHIHEE
Sbjct: 1021 SVINENLSVYLQFQGAISAEAELEKIKKKMEEVRKQQEKLKKMMDASGYKEKVRPHIHEE 1080
Query: 1081 NVNKLASLMQELLSLEEAGQHIQAHR 1107
NVNKLASLMQELLSLEEAGQHIQAHR
Sbjct: 1081 NVNKLASLMQELLSLEEAGQHIQAHR 1106
BLAST of Carg08430 vs. ExPASy TrEMBL
Match:
A0A6J1JYM1 (Valyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111490926 PE=3 SV=1)
HSP 1 Score: 2208.3 bits (5721), Expect = 0.0e+00
Identity = 1097/1107 (99.10%), Postives = 1101/1107 (99.46%), Query Frame = 0
Query: 1 MRNGILNLLAVCVKQGSVLGEPLLVSLFSRQSYKLSCVHRAPFSSFSKGICP-EFSVAPL 60
MRNGILNLLAVCVKQG VLGEPLLVSLFSRQSYKLSCVHRAPFSSFSKGICP EFSVAP
Sbjct: 1 MRNGILNLLAVCVKQGRVLGEPLLVSLFSRQSYKLSCVHRAPFSSFSKGICPSEFSVAPH 60
Query: 61 KDMESVKKPEVEDPEKKKKKEEKAREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARR 120
KDMESVKKPEVEDPEKKKKKEEKAREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARR
Sbjct: 61 KDMESVKKPEVEDPEKKKKKEEKAREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARR 120
Query: 121 GGDEENAEDFVDPDTPFGNKKLMSQQMSKQYNPSAVEKSWYEWWEKSGFFVADAKSSKPP 180
GGDEENAEDFVDPDTPFG KKLMSQQMSKQYNPSAVEKSWYEWWEKSGFFVADAKSSKPP
Sbjct: 121 GGDEENAEDFVDPDTPFGKKKLMSQQMSKQYNPSAVEKSWYEWWEKSGFFVADAKSSKPP 180
Query: 181 FVIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKI 240
FVIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKI
Sbjct: 181 FVIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKI 240
Query: 241 MRERNLTRHDIGREKFISEVWDWKTKYGGTILKQLRRLGASLDWTRECFTMDEKRSRAVT 300
MRERNLTRHDIGREKFISEVWDWKTKYGGTILKQLRRLGASLDWTRECFTMDEKRSRAVT
Sbjct: 241 MRERNLTRHDIGREKFISEVWDWKTKYGGTILKQLRRLGASLDWTRECFTMDEKRSRAVT 300
Query: 301 EAFVRLFKNGLIYRDLRLVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYEKPVEFGVLT 360
EAFVRLFKNGLIYRDLRLVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYEKPVEFGVLT
Sbjct: 301 EAFVRLFKNGLIYRDLRLVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYEKPVEFGVLT 360
Query: 361 SFAYPLEGELGEIVVATTRVETMLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPIICD 420
SFAYPLEGELGEIVVATTRVETMLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPIICD
Sbjct: 361 SFAYPLEGELGEIVVATTRVETMLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPIICD 420
Query: 421 AILVDPNFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFEGMPRFKV 480
AILVDPNFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFEGMPRFKV
Sbjct: 421 AILVDPNFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFEGMPRFKV 480
Query: 481 REMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAVMDN 540
REMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAV DN
Sbjct: 481 REMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAVTDN 540
Query: 541 ECKKIEIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATLDDDQLKEFGAYNDHW 600
ECKKI+IIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATLDDDQLKEFGAYNDHW
Sbjct: 541 ECKKIKIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATLDDDQLKEFGAYNDHW 600
Query: 601 VVARNEEEAEEEAKLIYAGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPDNTEDLKAFYPT 660
VVARNEEEAEEEAKLIYAGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPDNTEDLKAFYPT
Sbjct: 601 VVARNEEEAEEEAKLIYAGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPDNTEDLKAFYPT 660
Query: 661 SALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEV 720
SALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEV
Sbjct: 661 SALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEV 720
Query: 721 INGISLEGLHKRLEEGNLDPKELAVAKEGQVKDFPNGISECGADALRFALISYTAQSDKI 780
INGISLEGLHKRLEEGNLDPKELAVAKEGQVKDFPNGISECGADALRFALISYTAQSDKI
Sbjct: 721 INGISLEGLHKRLEEGNLDPKELAVAKEGQVKDFPNGISECGADALRFALISYTAQSDKI 780
Query: 781 NLDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVPSKNVTPDALPFSCQWILSVLNKAISR 840
NLDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVPSKNVTPDALPFSCQWILSVLNKAISR
Sbjct: 781 NLDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVPSKNVTPDALPFSCQWILSVLNKAISR 840
Query: 841 TVSSLEAYEFSDATTAVYSWWQYQLCDVFIEAIKPYFSANDTEYASARSHAQDTLWLCLE 900
TVSSLEAYEFSDATTAVY WWQYQLCDVFIEAIKPYFS+NDTEYASARSHAQDTLWLCLE
Sbjct: 841 TVSSLEAYEFSDATTAVYYWWQYQLCDVFIEAIKPYFSSNDTEYASARSHAQDTLWLCLE 900
Query: 901 NGLRLLHPFMPYVTEELWQRLPYPKSSTRPESIMICDYPSVTEEWTNESVETEMDLIVSA 960
NGLRLLHPFMPYVTEELWQRLPYPKSSTRPESIMICDYPSVTEEWT+ESVETEMDLIVSA
Sbjct: 901 NGLRLLHPFMPYVTEELWQRLPYPKSSTRPESIMICDYPSVTEEWTSESVETEMDLIVSA 960
Query: 961 VKSLRSLAKESRERRPGYVLPRNDVVAETINKRKLEIVTLANLSSLTVINDNDAAPVGCA 1020
VKSLRSLAKESRERRPGY+LPRNDVVAETINKRKLEIVTLANLSSLTVINDNDAAPVGCA
Sbjct: 961 VKSLRSLAKESRERRPGYMLPRNDVVAETINKRKLEIVTLANLSSLTVINDNDAAPVGCA 1020
Query: 1021 VSVVNENLSVYLQFQGAISAEAELEKIKKKMEEVRKQQEKLKKMMDASGYKEKVRPHIHE 1080
VSVVNENLSVYLQFQGAISAEAELEKIKKKMEEVRKQQEKLKKMMDASGYKEKVRPHIHE
Sbjct: 1021 VSVVNENLSVYLQFQGAISAEAELEKIKKKMEEVRKQQEKLKKMMDASGYKEKVRPHIHE 1080
Query: 1081 ENVNKLASLMQELLSLEEAGQHIQAHR 1107
ENVNKLASLMQELLSLEEAGQHIQAHR
Sbjct: 1081 ENVNKLASLMQELLSLEEAGQHIQAHR 1107
BLAST of Carg08430 vs. ExPASy TrEMBL
Match:
A0A1S3CT04 (Valyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103504618 PE=3 SV=1)
HSP 1 Score: 2035.8 bits (5273), Expect = 0.0e+00
Identity = 996/1045 (95.31%), Postives = 1027/1045 (98.28%), Query Frame = 0
Query: 62 MESVKKPEVEDPEKKKKKEEKAREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRGG 121
ME KKPE+EDPEKKKKKEEKAREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRGG
Sbjct: 1 MEPDKKPEMEDPEKKKKKEEKAREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRGG 60
Query: 122 DEENAEDFVDPDTPFGNKKLMSQQMSKQYNPSAVEKSWYEWWEKSGFFVADAKSSKPPFV 181
DEENAEDFVDPDTPFG KKL+SQQM+KQYNPSAVEKSWYEWWEKSG+FVADAKSSKPPFV
Sbjct: 61 DEENAEDFVDPDTPFGKKKLLSQQMAKQYNPSAVEKSWYEWWEKSGYFVADAKSSKPPFV 120
Query: 182 IVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMR 241
IVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSG+NTLWVPGTDHAGIATQVVVEKKIMR
Sbjct: 121 IVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGFNTLWVPGTDHAGIATQVVVEKKIMR 180
Query: 242 ERNLTRHDIGREKFISEVWDWKTKYGGTILKQLRRLGASLDWTRECFTMDEKRSRAVTEA 301
ERNLTRHDIGREKFISEVW+WKTKYGGTILKQLRRLGASLDWTRECFTMDEKRSRAVTEA
Sbjct: 181 ERNLTRHDIGREKFISEVWEWKTKYGGTILKQLRRLGASLDWTRECFTMDEKRSRAVTEA 240
Query: 302 FVRLFKNGLIYRDLRLVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYEKPVEFGVLTSF 361
FVRLFKNGLIYRDLRLVNWDCVLRTAISDIEVDYIDIKEKTLL+VPGYE PVEFGVLTSF
Sbjct: 241 FVRLFKNGLIYRDLRLVNWDCVLRTAISDIEVDYIDIKEKTLLRVPGYENPVEFGVLTSF 300
Query: 362 AYPLEGELGEIVVATTRVETMLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPIICDAI 421
AYPLEGELGEIVVATTRVETMLGDTAIA+HP+DTRYKHLHGKFAIHPFNGRKLPIICDAI
Sbjct: 301 AYPLEGELGEIVVATTRVETMLGDTAIAVHPEDTRYKHLHGKFAIHPFNGRKLPIICDAI 360
Query: 422 LVDPNFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFEGMPRFKVRE 481
LVDP FGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEF GMPRFK RE
Sbjct: 361 LVDPKFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFAGMPRFKARE 420
Query: 482 MVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAVMDNEC 541
MVVDALQKKGLYRGAKDNEMRLGLCSR+NDVVEPMIKPQWYVNCK+ AKQSLDA MDNEC
Sbjct: 421 MVVDALQKKGLYRGAKDNEMRLGLCSRTNDVVEPMIKPQWYVNCKEPAKQSLDAAMDNEC 480
Query: 542 KKIEIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATLDDDQLKEFGAYNDHWVV 601
KKI+IIPKQYSADWKRWL+NIRDWCISRQLWWGHRIPAWYATL+DDQLKEFGAYNDHWVV
Sbjct: 481 KKIDIIPKQYSADWKRWLDNIRDWCISRQLWWGHRIPAWYATLEDDQLKEFGAYNDHWVV 540
Query: 602 ARNEEEAEEEAKLIYAGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPDNTEDLKAFYPTSA 661
ARNEEEAEEEAK IYAGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPD+TEDLKAFYPTSA
Sbjct: 541 ARNEEEAEEEAKRIYAGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPDDTEDLKAFYPTSA 600
Query: 662 LETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVIN 721
LETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVIN
Sbjct: 601 LETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVIN 660
Query: 722 GISLEGLHKRLEEGNLDPKELAVAKEGQVKDFPNGISECGADALRFALISYTAQSDKINL 781
GISLEGLHKRLEEGNLDPKELA+AKEGQVKDFPNGISECGADALRFALISYTAQSDKINL
Sbjct: 661 GISLEGLHKRLEEGNLDPKELAIAKEGQVKDFPNGISECGADALRFALISYTAQSDKINL 720
Query: 782 DIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVPSKNVTPDALPFSCQWILSVLNKAISRTV 841
DIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVP+KN TPD LPFSCQWILSVLNKAISRT+
Sbjct: 721 DIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVPNKNATPDVLPFSCQWILSVLNKAISRTI 780
Query: 842 SSLEAYEFSDATTAVYSWWQYQLCDVFIEAIKPYFSANDTEYASARSHAQDTLWLCLENG 901
SSLE+YEFSDATTAVYSWWQYQLCDVFIEAIKPYFS+N+T+YASARSHAQDTLWLCLENG
Sbjct: 781 SSLESYEFSDATTAVYSWWQYQLCDVFIEAIKPYFSSNETDYASARSHAQDTLWLCLENG 840
Query: 902 LRLLHPFMPYVTEELWQRLPYPKSSTRPESIMICDYPSVTEEWTNESVETEMDLIVSAVK 961
LRLLHPFMPYVTEELWQRLPYPK+STRPESIMICDYPSVTEEWTNE+VE EMDLIVSAV+
Sbjct: 841 LRLLHPFMPYVTEELWQRLPYPKNSTRPESIMICDYPSVTEEWTNEAVENEMDLIVSAVR 900
Query: 962 SLRSLAKESRERRPGYVLPRNDVVAETINKRKLEIVTLANLSSLTVINDNDAAPVGCAVS 1021
SLRSLAKESRER+PGYVLPRN VAE INKRKLEIVTLANLSSLTVINDNDAAPVGCAVS
Sbjct: 901 SLRSLAKESRERKPGYVLPRNLAVAEIINKRKLEIVTLANLSSLTVINDNDAAPVGCAVS 960
Query: 1022 VVNENLSVYLQFQGAISAEAELEKIKKKMEEVRKQQEKLKKMMDASGYKEKVRPHIHEEN 1081
VVNENLSVYLQFQGAISAEAELEKI KK++E++KQQE+LKKMMDASGYKEKVRP IHEEN
Sbjct: 961 VVNENLSVYLQFQGAISAEAELEKINKKIDEIKKQQERLKKMMDASGYKEKVRPQIHEEN 1020
Query: 1082 VNKLASLMQELLSLEEAGQHIQAHR 1107
VNKLASLMQELLSLEEAGQHIQAHR
Sbjct: 1021 VNKLASLMQELLSLEEAGQHIQAHR 1045
BLAST of Carg08430 vs. ExPASy TrEMBL
Match:
A0A5D3BM83 (Valyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1728G00290 PE=3 SV=1)
HSP 1 Score: 2010.0 bits (5206), Expect = 0.0e+00
Identity = 979/1025 (95.51%), Postives = 1009/1025 (98.44%), Query Frame = 0
Query: 82 KAREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRGGDEENAEDFVDPDTPFGNKKL 141
+AREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRGGDEENAEDFVDPDTPFG KKL
Sbjct: 6 RAREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRGGDEENAEDFVDPDTPFGKKKL 65
Query: 142 MSQQMSKQYNPSAVEKSWYEWWEKSGFFVADAKSSKPPFVIVLPPPNVTGALHIGHALTA 201
+SQQM+KQYNPSAVEKSWYEWWEKSG+FVADAKSSKPPFVIVLPPPNVTGALHIGHALTA
Sbjct: 66 LSQQMAKQYNPSAVEKSWYEWWEKSGYFVADAKSSKPPFVIVLPPPNVTGALHIGHALTA 125
Query: 202 AIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKFISEVWD 261
AIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKFISEVW+
Sbjct: 126 AIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKFISEVWE 185
Query: 262 WKTKYGGTILKQLRRLGASLDWTRECFTMDEKRSRAVTEAFVRLFKNGLIYRDLRLVNWD 321
WKTKYGGTILKQLRRLGASLDWTRECFTMDEKRSRAVTEAFVRLFKNGLIYRDLRLVNWD
Sbjct: 186 WKTKYGGTILKQLRRLGASLDWTRECFTMDEKRSRAVTEAFVRLFKNGLIYRDLRLVNWD 245
Query: 322 CVLRTAISDIEVDYIDIKEKTLLKVPGYEKPVEFGVLTSFAYPLEGELGEIVVATTRVET 381
CVLRTAISDIEVDYIDIKEKTLL+VPGYE PVEFGVLTSFAYPLEGELGEIVVATTRVET
Sbjct: 246 CVLRTAISDIEVDYIDIKEKTLLRVPGYENPVEFGVLTSFAYPLEGELGEIVVATTRVET 305
Query: 382 MLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPIICDAILVDPNFGTGAVKITPAHDPN 441
MLGDTAIA+HP+DTRYKHLHGK AIHPFNGRKLPIICDAILVDP FGTGAVKITPAHDPN
Sbjct: 306 MLGDTAIAVHPEDTRYKHLHGKSAIHPFNGRKLPIICDAILVDPKFGTGAVKITPAHDPN 365
Query: 442 DFEVGKRHNLEFINIFTDDGKINSNGGSEFEGMPRFKVREMVVDALQKKGLYRGAKDNEM 501
DFEVGKRHNLEFINIFTDDGKINSNGGSEF GMPRFK REMVVDALQKKGLYRGAKDNEM
Sbjct: 366 DFEVGKRHNLEFINIFTDDGKINSNGGSEFAGMPRFKAREMVVDALQKKGLYRGAKDNEM 425
Query: 502 RLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAVMDNECKKIEIIPKQYSADWKRWLEN 561
RLGLCSR+NDVVEPMIKPQWYVNCK+ AKQSLDA MDNECKKI+IIPKQYSADWKRWL+N
Sbjct: 426 RLGLCSRTNDVVEPMIKPQWYVNCKEPAKQSLDAAMDNECKKIDIIPKQYSADWKRWLDN 485
Query: 562 IRDWCISRQLWWGHRIPAWYATLDDDQLKEFGAYNDHWVVARNEEEAEEEAKLIYAGKKF 621
IRDWCISRQLWWGHRIPAWYATL+DDQLKEFGAYNDHWVVARNEEEAEEEAK IYAGKKF
Sbjct: 486 IRDWCISRQLWWGHRIPAWYATLEDDQLKEFGAYNDHWVVARNEEEAEEEAKRIYAGKKF 545
Query: 622 HLTQDPDVLDTWFSSGLFPLSVLGWPDNTEDLKAFYPTSALETGHDILFFWVARMVMLGI 681
HLTQDPDVLDTWFSSGLFPLSVLGWPD+TEDLKAFYPTSALETGHDILFFWVARMVMLGI
Sbjct: 546 HLTQDPDVLDTWFSSGLFPLSVLGWPDDTEDLKAFYPTSALETGHDILFFWVARMVMLGI 605
Query: 682 TLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGNLDPKE 741
TLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGNLDPKE
Sbjct: 606 TLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGNLDPKE 665
Query: 742 LAVAKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAIR 801
LA+AKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAIR
Sbjct: 666 LAIAKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAIR 725
Query: 802 FAMSKLGNDYVPSKNVTPDALPFSCQWILSVLNKAISRTVSSLEAYEFSDATTAVYSWWQ 861
FAMSKLGNDYVP+KNVTPD LPFSCQWILSVLNKAISRT+SSLE+YEFSDATTAVYSWWQ
Sbjct: 726 FAMSKLGNDYVPNKNVTPDVLPFSCQWILSVLNKAISRTISSLESYEFSDATTAVYSWWQ 785
Query: 862 YQLCDVFIEAIKPYFSANDTEYASARSHAQDTLWLCLENGLRLLHPFMPYVTEELWQRLP 921
YQLCDVFIEAIKPYFS+N+T+YASARSHAQDTLWLCLENGLRLLHPFMPYVTEELWQRLP
Sbjct: 786 YQLCDVFIEAIKPYFSSNETDYASARSHAQDTLWLCLENGLRLLHPFMPYVTEELWQRLP 845
Query: 922 YPKSSTRPESIMICDYPSVTEEWTNESVETEMDLIVSAVKSLRSLAKESRERRPGYVLPR 981
YPK+STRPESIMICDYPSVTEEWTNE+VE EMDLIVSAV+SLRSLAKESRER+PGYVLPR
Sbjct: 846 YPKNSTRPESIMICDYPSVTEEWTNEAVENEMDLIVSAVRSLRSLAKESRERKPGYVLPR 905
Query: 982 NDVVAETINKRKLEIVTLANLSSLTVINDNDAAPVGCAVSVVNENLSVYLQFQGAISAEA 1041
N VAE INKRKLEIVTLANLSSLTVINDNDAAPVGCAVSVVNENLSVYLQFQGAISAEA
Sbjct: 906 NLAVAEIINKRKLEIVTLANLSSLTVINDNDAAPVGCAVSVVNENLSVYLQFQGAISAEA 965
Query: 1042 ELEKIKKKMEEVRKQQEKLKKMMDASGYKEKVRPHIHEENVNKLASLMQELLSLEEAGQH 1101
ELEKI KK++E++KQQE+LKKMMDASGYKEKVRP IHEENVNKLASLMQELLSLEEAGQH
Sbjct: 966 ELEKINKKIDEIKKQQERLKKMMDASGYKEKVRPQIHEENVNKLASLMQELLSLEEAGQH 1025
Query: 1102 IQAHR 1107
IQAHR
Sbjct: 1026 IQAHR 1030
BLAST of Carg08430 vs. ExPASy TrEMBL
Match:
A0A0A0KPW4 (Valyl-tRNA synthetase OS=Cucumis sativus OX=3659 GN=Csa_5G174580 PE=3 SV=1)
HSP 1 Score: 2006.9 bits (5198), Expect = 0.0e+00
Identity = 977/1025 (95.32%), Postives = 1008/1025 (98.34%), Query Frame = 0
Query: 82 KAREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRGGDEENAEDFVDPDTPFGNKKL 141
+AREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRGGDEENAEDFVDPDTPFG KKL
Sbjct: 2 QAREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRGGDEENAEDFVDPDTPFGKKKL 61
Query: 142 MSQQMSKQYNPSAVEKSWYEWWEKSGFFVADAKSSKPPFVIVLPPPNVTGALHIGHALTA 201
++QQM+KQYNPSAVEKSWYEWWEKSG+FVADAKSSKPPFVIVLPPPNVTGALHIGHALTA
Sbjct: 62 LAQQMAKQYNPSAVEKSWYEWWEKSGYFVADAKSSKPPFVIVLPPPNVTGALHIGHALTA 121
Query: 202 AIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKFISEVWD 261
AIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHD+GREKFISEVW+
Sbjct: 122 AIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDLGREKFISEVWE 181
Query: 262 WKTKYGGTILKQLRRLGASLDWTRECFTMDEKRSRAVTEAFVRLFKNGLIYRDLRLVNWD 321
WKTKYGGTILKQLRRLGASLDWTRECFTMDEKRSRAVTEAFVRLFKNGLIYRDLRLVNWD
Sbjct: 182 WKTKYGGTILKQLRRLGASLDWTRECFTMDEKRSRAVTEAFVRLFKNGLIYRDLRLVNWD 241
Query: 322 CVLRTAISDIEVDYIDIKEKTLLKVPGYEKPVEFGVLTSFAYPLEGELGEIVVATTRVET 381
CVLRTAISDIEVDYIDIKEKTLLKVPGYE PVEFGVLTSFAYPLEGELGEIVVATTRVET
Sbjct: 242 CVLRTAISDIEVDYIDIKEKTLLKVPGYENPVEFGVLTSFAYPLEGELGEIVVATTRVET 301
Query: 382 MLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPIICDAILVDPNFGTGAVKITPAHDPN 441
MLGDTAIAIHP+DTRYKHLHGK AIHPFNGRKLPI+CDAILVDP FGTGAVKITPAHDPN
Sbjct: 302 MLGDTAIAIHPEDTRYKHLHGKSAIHPFNGRKLPIVCDAILVDPKFGTGAVKITPAHDPN 361
Query: 442 DFEVGKRHNLEFINIFTDDGKINSNGGSEFEGMPRFKVREMVVDALQKKGLYRGAKDNEM 501
DFEVGKRHNLEFINIFTDDGKINSNGGSEF GMPRFK REMVVDALQKKGLYRGAKDNEM
Sbjct: 362 DFEVGKRHNLEFINIFTDDGKINSNGGSEFAGMPRFKAREMVVDALQKKGLYRGAKDNEM 421
Query: 502 RLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAVMDNECKKIEIIPKQYSADWKRWLEN 561
RLGLCSR+NDVVEPMIKPQWYVNCK+ AKQSLDA MDNECKKI+IIPKQYSADWKRWL+N
Sbjct: 422 RLGLCSRTNDVVEPMIKPQWYVNCKEPAKQSLDAAMDNECKKIDIIPKQYSADWKRWLDN 481
Query: 562 IRDWCISRQLWWGHRIPAWYATLDDDQLKEFGAYNDHWVVARNEEEAEEEAKLIYAGKKF 621
IRDWCISRQLWWGHRIPAWYA L+DDQLKEFGAYNDHWVVARNEEEAEEEAK IYAGKKF
Sbjct: 482 IRDWCISRQLWWGHRIPAWYAALEDDQLKEFGAYNDHWVVARNEEEAEEEAKRIYAGKKF 541
Query: 622 HLTQDPDVLDTWFSSGLFPLSVLGWPDNTEDLKAFYPTSALETGHDILFFWVARMVMLGI 681
HLTQDPDVLDTWFSSGLFPLSVLGWPD+TEDLKAFYPTSALETGHDI+FFWVARMVMLGI
Sbjct: 542 HLTQDPDVLDTWFSSGLFPLSVLGWPDDTEDLKAFYPTSALETGHDIIFFWVARMVMLGI 601
Query: 682 TLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGNLDPKE 741
TLGGDVPF+TIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGNLDPKE
Sbjct: 602 TLGGDVPFQTIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGNLDPKE 661
Query: 742 LAVAKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAIR 801
LA+AKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAIR
Sbjct: 662 LAIAKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAIR 721
Query: 802 FAMSKLGNDYVPSKNVTPDALPFSCQWILSVLNKAISRTVSSLEAYEFSDATTAVYSWWQ 861
FAMSKLGNDYVP+KNVTPDALPFSCQWILSVLNKAISRT+SSLE+YEFSDATTAVYSWWQ
Sbjct: 722 FAMSKLGNDYVPNKNVTPDALPFSCQWILSVLNKAISRTISSLESYEFSDATTAVYSWWQ 781
Query: 862 YQLCDVFIEAIKPYFSANDTEYASARSHAQDTLWLCLENGLRLLHPFMPYVTEELWQRLP 921
YQLCDVFIEAIKPYFS+N+T++ASARSHAQDTLWLCLENGLRLLHPFMPYVTEELWQRLP
Sbjct: 782 YQLCDVFIEAIKPYFSSNETDFASARSHAQDTLWLCLENGLRLLHPFMPYVTEELWQRLP 841
Query: 922 YPKSSTRPESIMICDYPSVTEEWTNESVETEMDLIVSAVKSLRSLAKESRERRPGYVLPR 981
YPK+STRPESIMICDYPSVTEEWTNE VE EMDLIVSAV+SLRSLAKESRERRPGYVLPR
Sbjct: 842 YPKNSTRPESIMICDYPSVTEEWTNEDVENEMDLIVSAVRSLRSLAKESRERRPGYVLPR 901
Query: 982 NDVVAETINKRKLEIVTLANLSSLTVINDNDAAPVGCAVSVVNENLSVYLQFQGAISAEA 1041
N VAETINKRKLEIVTLANLSSLTVINDNDAAPVGCAVSVVNENLSVYLQFQGAISAEA
Sbjct: 902 NAAVAETINKRKLEIVTLANLSSLTVINDNDAAPVGCAVSVVNENLSVYLQFQGAISAEA 961
Query: 1042 ELEKIKKKMEEVRKQQEKLKKMMDASGYKEKVRPHIHEENVNKLASLMQELLSLEEAGQH 1101
ELEKI KKM+E++KQQEKLKKMMDASGYKEKVRP IHEENVNKLASLMQELLSLEEAG H
Sbjct: 962 ELEKINKKMDEIKKQQEKLKKMMDASGYKEKVRPQIHEENVNKLASLMQELLSLEEAGLH 1021
Query: 1102 IQAHR 1107
I+AHR
Sbjct: 1022 IKAHR 1026
BLAST of Carg08430 vs. TAIR 10
Match:
AT1G14610.1 (valyl-tRNA synthetase / valine--tRNA ligase (VALRS) )
HSP 1 Score: 1606.7 bits (4159), Expect = 0.0e+00
Identity = 774/1054 (73.43%), Postives = 895/1054 (84.91%), Query Frame = 0
Query: 63 ESVKKPEVEDP-EKKKKKEEKAREKELKKKKALEK----ALKL-QAQQTSNAPKKSEKKN 122
ES KK E+ E+KKKKEEKA+EKELKK+KALEK LK QA+ +N PKKS KK+
Sbjct: 47 ESEKKILTEEELERKKKKEEKAKEKELKKQKALEKERLAELKAKQAKDGTNVPKKSAKKS 106
Query: 123 ARRGGDEENAEDFVDPDTPFGNKKLMSQQMSKQYNPSAVEKSWYEWWEKSGFFVADAKSS 182
++R EEN EDFVDP+TP G +K +S QM+KQY+P+ VEKSWY WWEKS F ADAKSS
Sbjct: 107 SKRDASEENPEDFVDPETPLGERKRLSSQMAKQYSPATVEKSWYAWWEKSDLFKADAKSS 166
Query: 183 KPPFVIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVE 242
KPPFVIVLPPPNVTGALHIGHALT+AIED IIRW+RMSGYN LWVPG DHAGIATQVVVE
Sbjct: 167 KPPFVIVLPPPNVTGALHIGHALTSAIEDTIIRWKRMSGYNALWVPGVDHAGIATQVVVE 226
Query: 243 KKIMRERNLTRHDIGREKFISEVWDWKTKYGGTILKQLRRLGASLDWTRECFTMDEKRSR 302
KKIMR+R +TRHD+GRE+F+ EVW WK +YGGTIL QLRRLGASLDW+RECFTMDE+RS+
Sbjct: 227 KKIMRDRGMTRHDVGREEFVKEVWKWKNQYGGTILTQLRRLGASLDWSRECFTMDEQRSK 286
Query: 303 AVTEAFVRLFKNGLIYRDLRLVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYEKPVEFG 362
AVTEAFVRL+K GLIYRD+RLVNWDC+LRTAISD+EV+YIDIKEKTLLKVPGYEKPVEFG
Sbjct: 287 AVTEAFVRLYKEGLIYRDIRLVNWDCILRTAISDVEVEYIDIKEKTLLKVPGYEKPVEFG 346
Query: 363 VLTSFAYPLEGELGEIVVATTRVETMLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPI 422
+LTSFAYPLEG LGE++VATTRVETMLGDTAIAIHPDD RYKHLHGKFA+HPFNGRKLPI
Sbjct: 347 LLTSFAYPLEGGLGEVIVATTRVETMLGDTAIAIHPDDARYKHLHGKFAVHPFNGRKLPI 406
Query: 423 ICDAILVDPNFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFEGMPR 482
ICD ILVDPNFGTG VKITPAHDPND EVGKRH LEFINIFTDDGKIN+NGGS+F GMPR
Sbjct: 407 ICDGILVDPNFGTGCVKITPAHDPNDCEVGKRHKLEFINIFTDDGKINTNGGSDFAGMPR 466
Query: 483 FKVREMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAV 542
F RE VV+ALQK+GLYRGAK+NEMRLGLCSR+NDV+EPMIKPQWYVNC + K++LD
Sbjct: 467 FAAREAVVEALQKQGLYRGAKNNEMRLGLCSRTNDVIEPMIKPQWYVNCSMIGKEALDVA 526
Query: 543 MDNECKKIEIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATLDDDQLKEFGAYN 602
+ +E KK+E +PKQY+A+W+RWLENIRDWCISRQLWWGHRIPAWYATL++DQLKE GAY+
Sbjct: 527 ITDENKKLEFVPKQYTAEWRRWLENIRDWCISRQLWWGHRIPAWYATLEEDQLKEVGAYS 586
Query: 603 DHWVVARNEEEAEEEAKLIYAGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPDNTEDLKAF 662
DHWVVAR E++A EEA + GKKF LT+DPDVLDTWFSSGLFPLSVLGWPD T+D KAF
Sbjct: 587 DHWVVARTEDDAREEAAQKFLGKKFELTRDPDVLDTWFSSGLFPLSVLGWPDVTDDFKAF 646
Query: 663 YPTSALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDP 722
YPTS LETGHDILFFWVARMVM+G+ LGG+VPF +Y HPMIRDAHGRKMSKSLGNVIDP
Sbjct: 647 YPTSVLETGHDILFFWVARMVMMGMKLGGEVPFSKVYFHPMIRDAHGRKMSKSLGNVIDP 706
Query: 723 VEVINGISLEGLHKRLEEGNLDPKELAVAKEGQVKDFPNGISECGADALRFALISYTAQS 782
+EVING++LEGLHKRLEEGNLDPKE+ VAKEGQVKDFPNGI ECG DALRFAL+SYTAQS
Sbjct: 707 LEVINGVTLEGLHKRLEEGNLDPKEVIVAKEGQVKDFPNGIPECGTDALRFALVSYTAQS 766
Query: 783 DKINLDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVPSKNVTPDALPFSCQWILSVLNKA 842
DKINLDI RVVGYRQWCNKLWNA+RFAM KLG+ Y P + ++P+ +PFSCQWILSVLNKA
Sbjct: 767 DKINLDILRVVGYRQWCNKLWNAVRFAMMKLGDGYTPPQTLSPETMPFSCQWILSVLNKA 826
Query: 843 ISRTVSSLEAYEFSDATTAVYSWWQYQLCDVFIEAIKPYFSANDTEYASARSHAQDTLWL 902
IS+TV SL+A+EFSDA +Y+WWQYQ CDV+IEAIKPYF+ ++ +AS R+HAQ LW+
Sbjct: 827 ISKTVVSLDAFEFSDAANTIYAWWQYQFCDVYIEAIKPYFAGDNPTFASERAHAQHALWI 886
Query: 903 CLENGLRLLHPFMPYVTEELWQRLPYPKSSTRPESIMICDYPSVTEEWTNESVETEMDLI 962
LE GLRLLHPFMP+VTEELWQRLP PK + R SIMICDYPS E W+NE VE+EMD +
Sbjct: 887 SLETGLRLLHPFMPFVTEELWQRLPAPKDTERKASIMICDYPSAIENWSNEKVESEMDTV 946
Query: 963 VSAVKSLRS-----LAKESRERRPGYVLPRNDVVAETINKRKLEIVTLANLSSLTVINDN 1022
++ VK +R+ L K+ ER P + L N+V +E + +LEI TLANLSSL V++
Sbjct: 947 LATVKCMRALRAGLLEKQKNERLPAFALCENNVTSEIVKSHELEIRTLANLSSLEVVSKG 1006
Query: 1023 D-AAPVGCAVSVVNENLSVYLQFQGAISAEAELEKIKKKMEEVRKQQEKLKKMMDASGYK 1082
AAP G +V VNENL VYL+ GAI+ EAE EKI+ K+ E++KQ+EKL+KMM S Y+
Sbjct: 1007 QHAAPPGSSVETVNENLKVYLEVDGAINTEAEQEKIRNKIGELQKQKEKLQKMMSVSTYE 1066
Query: 1083 EKVRPHIHEENVNKLASLMQELLSLEEAGQHIQA 1105
EKV +I E+N NKLA ++QE E+ + A
Sbjct: 1067 EKVPANIKEDNANKLAKILQEFDFFEKESARLAA 1100
BLAST of Carg08430 vs. TAIR 10
Match:
AT5G16715.1 (ATP binding;valine-tRNA ligases;aminoacyl-tRNA ligases;nucleotide binding;ATP binding;aminoacyl-tRNA ligases )
HSP 1 Score: 651.7 bits (1680), Expect = 1.0e-186
Identity = 384/980 (39.18%), Postives = 550/980 (56.12%), Query Frame = 0
Query: 137 GNKKLMSQQMSKQYNPSAVEKSWYEWWEKSGFFVADAKSSKPPFVIVLPPPNVTGALHIG 196
GN S + SK ++ S+ EK Y+WWE G+F + PFVI +PPPNVTG+LH+G
Sbjct: 60 GNNVFTSPETSKTFDFSSEEKI-YKWWESQGYFKPNFDQGGSPFVIPMPPPNVTGSLHMG 119
Query: 197 HALTAAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKFI 256
HA+ +ED ++R+ RM+G TLW+PGTDHAGIATQ+VVEK + E + R D+GR++F
Sbjct: 120 HAMFVTLEDIMVRYNRMNGRPTLWLPGTDHAGIATQLVVEKMLASE-GIKRVDLGRDEFT 179
Query: 257 SEVWDWKTKYGGTILKQLRRLGASLDWTRECFTMDEKRSRAVTEAFVRLFKNGLIYRDLR 316
VW+WK KYGGTI Q++RLGAS DW+RE FT+DE+ SRAV EAFV+L GLIY+
Sbjct: 180 KRVWEWKEKYGGTITNQIKRLGASCDWSRERFTLDEQLSRAVVEAFVKLHDKGLIYQGSY 239
Query: 317 LVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYEKPVEFGVLTSFAYPLEGELGEIVVAT 376
+VNW L+TA+SD+EV+Y + E G L Y + G + +AT
Sbjct: 240 MVNWSPNLQTAVSDLEVEYSE----------------EPGFLYHIKYRVAGSPDFLTIAT 299
Query: 377 TRVETMLGDTAIAIHPDDTRYKHLHGKFAIHPFN-GRKLPIICDAILVDPNFGTGAVKIT 436
TR ET+ GD A+A+HP+D RY G+ AI P GR +PII D VD +FGTG +KI+
Sbjct: 300 TRPETLFGDVALAVHPEDDRYSKYVGQTAIVPMTYGRHVPIIADK-YVDKDFGTGVLKIS 359
Query: 437 PAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFEGMPRFKVREMVVDALQKKGLYRG 496
P HD ND+ + ++ L +N+ D +N G F G+ RF+VRE + L++ GL
Sbjct: 360 PGHDHNDYLLARKLGLPILNVMNKDATLNDVAGL-FCGLDRFEVREKLWADLEEIGLAVK 419
Query: 497 AKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAVMDNECKKIEIIPKQYSADW 556
+ + +R+ R +V+EP++ QW+V+ +A+++L AV E K++ IIP+++ +
Sbjct: 420 KEPHTLRVPRSQRGGEVIEPLVSKQWFVHMDPLAEKALLAV---ENKELTIIPERFEKIY 479
Query: 557 KRWLENIRDWCISRQLWWGHRIPAWYATLDDDQLKEFGAYNDHWVVARNEEEAEEEAKLI 616
WL NI+DWCISRQLWWGHRIP WY D + + ++VA++ EEA E+A
Sbjct: 480 NHWLTNIKDWCISRQLWWGHRIPVWYVVGKDCE--------EDYIVAKSAEEALEKALEK 539
Query: 617 YAGKKFHLTQDPDVLDTWFSSGLFPLSVLGWPD-NTEDLKAFYPTSALETGHDILFFWVA 676
Y GK + QDPDVLDTWFSS L+P S LGWPD +D FYPT+ LETGHDILFFWVA
Sbjct: 540 Y-GKDVEIYQDPDVLDTWFSSSLWPFSTLGWPDVAAKDFNNFYPTNMLETGHDILFFWVA 599
Query: 677 RMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEE 736
RMVM+GI G VPF +YLH +IRD+ GRKMSKSLGNVIDP++ I
Sbjct: 600 RMVMMGIEFTGTVPFSHVYLHGLIRDSQGRKMSKSLGNVIDPLDTI-------------- 659
Query: 737 GNLDPKELAVAKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCN 796
KDF G DALRF + TA D +NL +R+ + + N
Sbjct: 660 ----------------KDF-------GTDALRFTIALGTAGQD-LNLSTERLTANKAFTN 719
Query: 797 KLWNAIRFAMSKLGN-----------DYVPSKNVTPDALPFSCQWILSVLNKAISRTVSS 856
KLWNA +F + L + D K T +LP W +S L+ I +S
Sbjct: 720 KLWNAGKFVLHSLPSLSDTSAWENLLDLKLDKEETLLSLPLPECWAVSKLHILIDSVTAS 779
Query: 857 LEAYEFSDATTAVYSWWQYQLCDVFIEAIKPYFSANDTEYASARSHAQDTLWLCLENGLR 916
E F D Y ++ D +IEA K + S S A L EN L+
Sbjct: 780 YEKLFFGDVGRETYDFFWSDFADWYIEASKSRLYGSGGNSVSLASQA--VLLYVFENILK 839
Query: 917 LLHPFMPYVTEELWQRLPYPKSSTRPESIMICDYPSVTEEWTNESVE--TEMDLIVSAVK 976
LLHPFMP+VTE+LWQ LPY R E++++ +P + ES++ + + A++
Sbjct: 840 LLHPFMPFVTEDLWQALPY-----RKEALIVSPWPQNSLPRNVESIKRFENLQALTRAIR 899
Query: 977 SLRSLAKESRERRPGYVLPRNDVVAETINKRKLEIVTLANLSSLTVINDNDAAPVGCAVS 1036
+ R+ +R + + V E I+K K E++ L + L ++ ++A P +S
Sbjct: 900 NARAEYSVEPVKRISASVVGSAEVIEYISKEK-EVLALLSRLDLNNVHFSNAPPGDANLS 959
Query: 1037 ---VVNENLSVYLQFQGAISAEAELEKIKKKMEEVRKQQEKLKKMMDASGYKEKVRPHIH 1096
V +E L YL + +E+++I K++ +++ + + L + + + EK P
Sbjct: 960 VHLVASEGLEAYLPLAAMVDISSEVQRISKRLSKMQTEYDALITRLSSPKFVEKA-PEEV 960
Query: 1097 EENVNKLASLMQELLSLEEA 1099
V + ++E + L +A
Sbjct: 1020 VRGVKEQVEELEEKIKLTKA 960
BLAST of Carg08430 vs. TAIR 10
Match:
AT5G49030.1 (tRNA synthetase class I (I, L, M and V) family protein )
HSP 1 Score: 189.5 bits (480), Expect = 1.4e-47
Identity = 205/916 (22.38%), Postives = 340/916 (37.12%), Query Frame = 0
Query: 108 APKKSEKKNARRGGDEENAEDFVDPDTPFGNKKLMSQQMSKQYNPSAVEKSWYEWWEKSG 167
A KK+ + + G + D P T FG + N E + WE++
Sbjct: 87 AAKKASEGEKQEDGKYKQTVDL--PKTGFGMRA----------NSLTREPELQKLWEENQ 146
Query: 168 FF--VADAKSSKPPFVIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGYNTLWVPGTD 227
F V+D ++ F++ PP G LH+GHAL ++D I R++ + Y +VPG D
Sbjct: 147 VFKRVSD-NNNGGSFILHDGPPYANGDLHMGHALNKILKDIINRYKLLQNYKVQYVPGWD 206
Query: 228 HAGIATQVVVEKKIMRERNLTRHDIGREKFISEVWDWKTKYGGTILKQLRRLGASLDWTR 287
G+ ++ V + + +E R ++ K ++ + T ++ +R G DW
Sbjct: 207 CHGLPIELKVLQSLDQE---VRKELTPLKLRAKAAKFAKATVKTQMESFKRFGVWADWNN 266
Query: 288 ECFTMDEKRSRAVTEAFVRLFKNGLIYRDLRLVNWDCVLRTAISDIEVDY----IDIKEK 347
T+D + A E F ++ G IYR + V+W RTA+++ E++Y I
Sbjct: 267 PYLTLDPEYEAAQIEVFGQMALKGYIYRGRKPVHWSPSSRTALAEAELEYPEGHISKSIY 326
Query: 348 TLLKVPGYEK---------------------------------PVEFGV----------- 407
+ K+ G K +++ V
Sbjct: 327 AIFKLVGGAKTSLLDEFIPNIYLAVWTTTPWTMPANAAVAVNAKLQYSVVEVQSFSEDES 386
Query: 408 -LTSFAYPLEGELGE------IVVATTRVETMLGDTAIAIHPDDTRY-KHLHGKFAIHPF 467
+TS + G++ + ++VAT V + + + T L HP
Sbjct: 387 TVTSNKKKIPGKVLKNQQKLFVIVATDLVPALEAKWGVKLSISKTFLGSDLENCRYTHPI 446
Query: 468 NGRKLPIICDAILVDPNFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGS 527
+ R P++ + GTG V P H D+ G ++ L ++ D+GK G
Sbjct: 447 DNRDCPVVIGGDYITTESGTGLVHTAPGHGQEDYATGLKYGLPLVSPVDDEGKFTEEAG- 506
Query: 528 EFEGMPRF-KVREMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDM 587
+F G+ + VV L + + + R+ QW+ + +
Sbjct: 507 QFRGLSVLGEGNTAVVSYLDENMSLVMEESYAHKYPYDWRTKKPTIFRATEQWFASVEGF 566
Query: 588 AKQSLDAVMDNECKKIEIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATLDDDQ 647
++DA+ ++ +P Q + DWCISRQ WG IPA+Y +
Sbjct: 567 RTATMDAI-----NNVKWVPHQAVNRISAMTSSRSDWCISRQRTWGVPIPAFYHVKTKEP 626
Query: 648 LKEFGAYN-----------DHWVVARNEEEAEEEAKLIYAGKKFHLTQDPDVLDTWFSSG 707
L N D W E+ E+ Y K + D +D WF SG
Sbjct: 627 LMNEETINHVKSIISQKGSDAWWYMSVEDLLPEK----YRDKAADYEKGTDTMDVWFDSG 686
Query: 708 LFPLSVLGWPDNTEDLKAFYPTSALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPM 767
VLG E L +P G D W ++ I G P+ + H
Sbjct: 687 SSWAGVLG---KREGLS--FPADVYLEGTDQHRGWFQSSLLTSIATQGKAPYSAVITHGF 746
Query: 768 IRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGNLDPKELAVAKEGQVKDFPNGI 827
+ D G KMSKSLGNV+DP VI G KD P
Sbjct: 747 VLDEKGMKMSKSLGNVVDPRLVIEG------------------------GKNSKDAP--- 806
Query: 828 SECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVPSKNV 887
GAD +R + S D + + Q + KL +R+ + L +D+ V
Sbjct: 807 -AYGADVMRLWVSSVDYTGD-VLIGPQILRQMSDIYRKLRGTLRYLLGNL-HDWRVDNAV 866
Query: 888 TPDALPFSCQWILSVLNKAISRTVSSLEAYEFSDATTAVYSWWQYQLCDVFIEAIKPYFS 947
LP Q L L + E Y+F + + L + + + K
Sbjct: 867 PYQDLPIIDQHALFQLENVVKNIQECYENYQFFKIFQIIQRFTIVDLSNFYFDIAKDRLY 926
Query: 948 ANDTEYASARSHAQDTLWLCLENGLRLLHPFMPYVTEELWQRLPYP---KSSTRPESIMI 951
T + RS Q L L + LR++ P +P++ E++WQ LP+ + + E +
Sbjct: 927 TGGTSSFTRRS-CQTVLSTHLLSILRVIAPIVPHLAEDVWQNLPFEYRNEDGSAAEFVFE 940
BLAST of Carg08430 vs. TAIR 10
Match:
AT5G49030.2 (tRNA synthetase class I (I, L, M and V) family protein )
HSP 1 Score: 189.5 bits (480), Expect = 1.4e-47
Identity = 205/916 (22.38%), Postives = 340/916 (37.12%), Query Frame = 0
Query: 108 APKKSEKKNARRGGDEENAEDFVDPDTPFGNKKLMSQQMSKQYNPSAVEKSWYEWWEKSG 167
A KK+ + + G + D P T FG + N E + WE++
Sbjct: 87 AAKKASEGEKQEDGKYKQTVDL--PKTGFGMRA----------NSLTREPELQKLWEENQ 146
Query: 168 FF--VADAKSSKPPFVIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGYNTLWVPGTD 227
F V+D ++ F++ PP G LH+GHAL ++D I R++ + Y +VPG D
Sbjct: 147 VFKRVSD-NNNGGSFILHDGPPYANGDLHMGHALNKILKDIINRYKLLQNYKVQYVPGWD 206
Query: 228 HAGIATQVVVEKKIMRERNLTRHDIGREKFISEVWDWKTKYGGTILKQLRRLGASLDWTR 287
G+ ++ V + + +E R ++ K ++ + T ++ +R G DW
Sbjct: 207 CHGLPIELKVLQSLDQE---VRKELTPLKLRAKAAKFAKATVKTQMESFKRFGVWADWNN 266
Query: 288 ECFTMDEKRSRAVTEAFVRLFKNGLIYRDLRLVNWDCVLRTAISDIEVDY----IDIKEK 347
T+D + A E F ++ G IYR + V+W RTA+++ E++Y I
Sbjct: 267 PYLTLDPEYEAAQIEVFGQMALKGYIYRGRKPVHWSPSSRTALAEAELEYPEGHISKSIY 326
Query: 348 TLLKVPGYEK---------------------------------PVEFGV----------- 407
+ K+ G K +++ V
Sbjct: 327 AIFKLVGGAKTSLLDEFIPNIYLAVWTTTPWTMPANAAVAVNAKLQYSVVEVQSFSEDES 386
Query: 408 -LTSFAYPLEGELGE------IVVATTRVETMLGDTAIAIHPDDTRY-KHLHGKFAIHPF 467
+TS + G++ + ++VAT V + + + T L HP
Sbjct: 387 TVTSNKKKIPGKVLKNQQKLFVIVATDLVPALEAKWGVKLSISKTFLGSDLENCRYTHPI 446
Query: 468 NGRKLPIICDAILVDPNFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGS 527
+ R P++ + GTG V P H D+ G ++ L ++ D+GK G
Sbjct: 447 DNRDCPVVIGGDYITTESGTGLVHTAPGHGQEDYATGLKYGLPLVSPVDDEGKFTEEAG- 506
Query: 528 EFEGMPRF-KVREMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDM 587
+F G+ + VV L + + + R+ QW+ + +
Sbjct: 507 QFRGLSVLGEGNTAVVSYLDENMSLVMEESYAHKYPYDWRTKKPTIFRATEQWFASVEGF 566
Query: 588 AKQSLDAVMDNECKKIEIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATLDDDQ 647
++DA+ ++ +P Q + DWCISRQ WG IPA+Y +
Sbjct: 567 RTATMDAI-----NNVKWVPHQAVNRISAMTSSRSDWCISRQRTWGVPIPAFYHVKTKEP 626
Query: 648 LKEFGAYN-----------DHWVVARNEEEAEEEAKLIYAGKKFHLTQDPDVLDTWFSSG 707
L N D W E+ E+ Y K + D +D WF SG
Sbjct: 627 LMNEETINHVKSIISQKGSDAWWYMSVEDLLPEK----YRDKAADYEKGTDTMDVWFDSG 686
Query: 708 LFPLSVLGWPDNTEDLKAFYPTSALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPM 767
VLG E L +P G D W ++ I G P+ + H
Sbjct: 687 SSWAGVLG---KREGLS--FPADVYLEGTDQHRGWFQSSLLTSIATQGKAPYSAVITHGF 746
Query: 768 IRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGNLDPKELAVAKEGQVKDFPNGI 827
+ D G KMSKSLGNV+DP VI G KD P
Sbjct: 747 VLDEKGMKMSKSLGNVVDPRLVIEG------------------------GKNSKDAP--- 806
Query: 828 SECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVPSKNV 887
GAD +R + S D + + Q + KL +R+ + L +D+ V
Sbjct: 807 -AYGADVMRLWVSSVDYTGD-VLIGPQILRQMSDIYRKLRGTLRYLLGNL-HDWRVDNAV 866
Query: 888 TPDALPFSCQWILSVLNKAISRTVSSLEAYEFSDATTAVYSWWQYQLCDVFIEAIKPYFS 947
LP Q L L + E Y+F + + L + + + K
Sbjct: 867 PYQDLPIIDQHALFQLENVVKNIQECYENYQFFKIFQIIQRFTIVDLSNFYFDIAKDRLY 926
Query: 948 ANDTEYASARSHAQDTLWLCLENGLRLLHPFMPYVTEELWQRLPYP---KSSTRPESIMI 951
T + RS Q L L + LR++ P +P++ E++WQ LP+ + + E +
Sbjct: 927 TGGTSSFTRRS-CQTVLSTHLLSILRVIAPIVPHLAEDVWQNLPFEYRNEDGSAAEFVFE 940
BLAST of Carg08430 vs. TAIR 10
Match:
AT5G49030.3 (tRNA synthetase class I (I, L, M and V) family protein )
HSP 1 Score: 189.5 bits (480), Expect = 1.4e-47
Identity = 205/916 (22.38%), Postives = 340/916 (37.12%), Query Frame = 0
Query: 108 APKKSEKKNARRGGDEENAEDFVDPDTPFGNKKLMSQQMSKQYNPSAVEKSWYEWWEKSG 167
A KK+ + + G + D P T FG + N E + WE++
Sbjct: 273 AAKKASEGEKQEDGKYKQTVDL--PKTGFGMRA----------NSLTREPELQKLWEENQ 332
Query: 168 FF--VADAKSSKPPFVIVLPPPNVTGALHIGHALTAAIEDAIIRWRRMSGYNTLWVPGTD 227
F V+D ++ F++ PP G LH+GHAL ++D I R++ + Y +VPG D
Sbjct: 333 VFKRVSD-NNNGGSFILHDGPPYANGDLHMGHALNKILKDIINRYKLLQNYKVQYVPGWD 392
Query: 228 HAGIATQVVVEKKIMRERNLTRHDIGREKFISEVWDWKTKYGGTILKQLRRLGASLDWTR 287
G+ ++ V + + +E R ++ K ++ + T ++ +R G DW
Sbjct: 393 CHGLPIELKVLQSLDQE---VRKELTPLKLRAKAAKFAKATVKTQMESFKRFGVWADWNN 452
Query: 288 ECFTMDEKRSRAVTEAFVRLFKNGLIYRDLRLVNWDCVLRTAISDIEVDY----IDIKEK 347
T+D + A E F ++ G IYR + V+W RTA+++ E++Y I
Sbjct: 453 PYLTLDPEYEAAQIEVFGQMALKGYIYRGRKPVHWSPSSRTALAEAELEYPEGHISKSIY 512
Query: 348 TLLKVPGYEK---------------------------------PVEFGV----------- 407
+ K+ G K +++ V
Sbjct: 513 AIFKLVGGAKTSLLDEFIPNIYLAVWTTTPWTMPANAAVAVNAKLQYSVVEVQSFSEDES 572
Query: 408 -LTSFAYPLEGELGE------IVVATTRVETMLGDTAIAIHPDDTRY-KHLHGKFAIHPF 467
+TS + G++ + ++VAT V + + + T L HP
Sbjct: 573 TVTSNKKKIPGKVLKNQQKLFVIVATDLVPALEAKWGVKLSISKTFLGSDLENCRYTHPI 632
Query: 468 NGRKLPIICDAILVDPNFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGS 527
+ R P++ + GTG V P H D+ G ++ L ++ D+GK G
Sbjct: 633 DNRDCPVVIGGDYITTESGTGLVHTAPGHGQEDYATGLKYGLPLVSPVDDEGKFTEEAG- 692
Query: 528 EFEGMPRF-KVREMVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDM 587
+F G+ + VV L + + + R+ QW+ + +
Sbjct: 693 QFRGLSVLGEGNTAVVSYLDENMSLVMEESYAHKYPYDWRTKKPTIFRATEQWFASVEGF 752
Query: 588 AKQSLDAVMDNECKKIEIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATLDDDQ 647
++DA+ ++ +P Q + DWCISRQ WG IPA+Y +
Sbjct: 753 RTATMDAI-----NNVKWVPHQAVNRISAMTSSRSDWCISRQRTWGVPIPAFYHVKTKEP 812
Query: 648 LKEFGAYN-----------DHWVVARNEEEAEEEAKLIYAGKKFHLTQDPDVLDTWFSSG 707
L N D W E+ E+ Y K + D +D WF SG
Sbjct: 813 LMNEETINHVKSIISQKGSDAWWYMSVEDLLPEK----YRDKAADYEKGTDTMDVWFDSG 872
Query: 708 LFPLSVLGWPDNTEDLKAFYPTSALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPM 767
VLG E L +P G D W ++ I G P+ + H
Sbjct: 873 SSWAGVLG---KREGLS--FPADVYLEGTDQHRGWFQSSLLTSIATQGKAPYSAVITHGF 932
Query: 768 IRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGNLDPKELAVAKEGQVKDFPNGI 827
+ D G KMSKSLGNV+DP VI G KD P
Sbjct: 933 VLDEKGMKMSKSLGNVVDPRLVIEG------------------------GKNSKDAP--- 992
Query: 828 SECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVPSKNV 887
GAD +R + S D + + Q + KL +R+ + L +D+ V
Sbjct: 993 -AYGADVMRLWVSSVDYTGD-VLIGPQILRQMSDIYRKLRGTLRYLLGNL-HDWRVDNAV 1052
Query: 888 TPDALPFSCQWILSVLNKAISRTVSSLEAYEFSDATTAVYSWWQYQLCDVFIEAIKPYFS 947
LP Q L L + E Y+F + + L + + + K
Sbjct: 1053 PYQDLPIIDQHALFQLENVVKNIQECYENYQFFKIFQIIQRFTIVDLSNFYFDIAKDRLY 1112
Query: 948 ANDTEYASARSHAQDTLWLCLENGLRLLHPFMPYVTEELWQRLPYP---KSSTRPESIMI 951
T + RS Q L L + LR++ P +P++ E++WQ LP+ + + E +
Sbjct: 1113 TGGTSSFTRRS-CQTVLSTHLLSILRVIAPIVPHLAEDVWQNLPFEYRNEDGSAAEFVFE 1126
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
KAG7035615.1 | 0.0e+00 | 100.00 | Valine--tRNA ligase, mitochondrial 1 [Cucurbita argyrosperma subsp. argyrosperma... | [more] |
XP_023533939.1 | 0.0e+00 | 99.46 | valine--tRNA ligase, mitochondrial 1 [Cucurbita pepo subsp. pepo] | [more] |
XP_022957954.1 | 0.0e+00 | 99.46 | valine--tRNA ligase, mitochondrial 1 isoform X2 [Cucurbita moschata] | [more] |
XP_022995352.1 | 0.0e+00 | 99.10 | valine--tRNA ligase, mitochondrial 1 [Cucurbita maxima] | [more] |
KAG6605705.1 | 0.0e+00 | 99.81 | Valine--tRNA ligase, mitochondrial 1, partial [Cucurbita argyrosperma subsp. sor... | [more] |
Match Name | E-value | Identity | Description | |
P93736 | 0.0e+00 | 73.43 | Valine--tRNA ligase, mitochondrial 1 OS=Arabidopsis thaliana OX=3702 GN=TWN2 PE=... | [more] |
Q86KU2 | 2.7e-301 | 50.90 | Probable valine--tRNA ligase, cytoplasmic OS=Dictyostelium discoideum OX=44689 G... | [more] |
Q9Z1Q9 | 1.3e-282 | 49.37 | Valine--tRNA ligase OS=Mus musculus OX=10090 GN=Vars1 PE=1 SV=1 | [more] |
P26640 | 1.7e-282 | 48.15 | Valine--tRNA ligase OS=Homo sapiens OX=9606 GN=VARS1 PE=1 SV=4 | [more] |
Q04462 | 2.8e-282 | 49.48 | Valine--tRNA ligase OS=Rattus norvegicus OX=10116 GN=Vars1 PE=2 SV=2 | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1H3K8 | 0.0e+00 | 99.46 | Valyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111459332 PE=3 SV=1 | [more] |
A0A6J1JYM1 | 0.0e+00 | 99.10 | Valyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111490926 PE=3 SV=1 | [more] |
A0A1S3CT04 | 0.0e+00 | 95.31 | Valyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103504618 PE=3 SV=1 | [more] |
A0A5D3BM83 | 0.0e+00 | 95.51 | Valyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold17... | [more] |
A0A0A0KPW4 | 0.0e+00 | 95.32 | Valyl-tRNA synthetase OS=Cucumis sativus OX=3659 GN=Csa_5G174580 PE=3 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
AT1G14610.1 | 0.0e+00 | 73.43 | valyl-tRNA synthetase / valine--tRNA ligase (VALRS) | [more] |
AT5G16715.1 | 1.0e-186 | 39.18 | ATP binding;valine-tRNA ligases;aminoacyl-tRNA ligases;nucleotide binding;ATP bi... | [more] |
AT5G49030.1 | 1.4e-47 | 22.38 | tRNA synthetase class I (I, L, M and V) family protein | [more] |
AT5G49030.2 | 1.4e-47 | 22.38 | tRNA synthetase class I (I, L, M and V) family protein | [more] |
AT5G49030.3 | 1.4e-47 | 22.38 | tRNA synthetase class I (I, L, M and V) family protein | [more] |