Carg08420 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg08420
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionPfkB domain-containing protein
LocationCarg_Chr02: 5845048 .. 5849333 (+)
RNA-Seq ExpressionCarg08420
SyntenyCarg08420
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAACTCTCGGAAGAAAAACAAGAATTAATATTGATCCATTTGTGGGGGAAGTAGGTTTATAAACTTAATTATCCGTTTCCCTACAGATGGGGGGAATGGGGCGGTGGGTGGCTCTGCTGAATTCCGCCATTTTTGCGTTTCTTTGATGCACCGATTCACCACCACTCGCTCCAAATCCCATTCGCTGCGATGTCTCTCTATTTTCCCATTTCTTCTCTTCCTCAATTTCACTTACCCTTTTCTTCTCAGCCTCTCTCTTATCCTAACTTGACTCCTCTTCATTCACTTCATTTTTCCTCTTCTTCATATCTCCCTTCTTCCTCTTCCACTTTCATTCACCGATGCACAGTTGGCGCCTGCTTCAGATTCTCTTCCATGGCCGGAGCCTTCGATTCTTTCACCGGTTTGTCTTCCTGTCGTGTTTTTAGCGGCGATGGGACAAGGGAGTTTTGTAGCGGGAGCGAGGAAGAAGAGGAGGAGGAGGGTGAGGGCAACGTCTCCGATGAAGAATTTGTGGTCGGAATTGAAGAATCTAATGACATAAATGGTTTGCCTGATCGATGGGATGTCTTGGGGCTTGGACAAGCTATGGTAATTTTCCTGTTTTTCATGCTGTTTCTTGAGGAAATTTCTGTATTGAGATTTAAATTATTGCGAGTTTGGTGTTTGATTCTCTCTTGTTGGATAACTTGCTGATTTGAGTGGTATTGAGCTGTTGTAGTTAGAGCAATATTAATGAACACATTGTTTATGTAAGCGTTAGAAATTTTGATTTGGTAGAGGTCAAGACGGTTCTAGGGAAACTTTCTTACGAGATGGAATGATGTTAAAAAAGAAAATCACCTATCTCATAGTCTTGTTTAGTGCTCAGGTACCACTACAAGATTGAATTTAGGCTAAAATAGGACAAGTTAAAAGTTCACACTGCAATGAGGTTGTAAAATATACCTCCCAGCCCTTCATCCCTAGGACGTAGGACTGGTCAAATGGTACTTTACACTCCCACTTTGGTATAACCTCCTAAGAGGAGTACTTGACAGCTGGTGGTGGGTTTTTGGGATGGTATGACTCGGCTCCCTAGCCCGTAGCAACCAGTACTTTCCACCGGGTTTTCCTATTTCAGGAGTATATGCAAGATGAAATGCTTAGGTGGAATGAGGTGGTGGGTGCTTATTTCAAACAGGTGAACCCGAAATTTCTACATATTCGATTTTGAACGAAGGAAACAAGAAAGTTGGTTCATTTTCAAGTCAAGTGAGAAGCCAAAAACCATAGGATCGAGGCTGATATTCCCCAGTGGAGTCACGAGCAGTGGCTCAAGCATAAGAAACAAATCCTTGTAACATGCTGCGCCACTAATATTTATAGATCATAATGTTCCCTAGGAGCAACTTCAGTTTCTTATTTGATGTAATTTGCAATGATGATTTGTGCTAATTCTTGTGTTCTTCATTGGTGTGGCAACATTATTTAGGTAGATTTCTCTGGTATGGTGGACGATGAGTTTCTGAAGAAGCTGGGACTAGAGAAGGGTACAAGGAAGCTTGTAAATCATGAGGAAAGAGGCAGAGTGTTGCGGGCGATGGATGGCCACAGCTATAAGGCGGCTGCTGGAGGGTCTCTTTCCAACAGCCTTGTGGCCTTGGCAAGGCTGGGTATTAGACCCATGAAAGGCCCTACTTTCAATGTAGCCATGACTGGTAGTGTAGGGAGTGATCCATTGGGCAGCTTCTACAGGTCAGGCTAGCCTCTAGTTTCACTTTATATTTTGTTATTTTGCATTCTCGATTTAAATTCGTTAGACGGCATGAAACTAAAGTACAAAGAAGTTGCGTGACAAGCTCATAGGCACCACGTTGATGGATCCATTTTCTTGGAGTGTCATTGTGAGATCCCACATCGATTGGAGAGGGGAACGAAGCTGTAACGACCCAGATCTATTGCTAGTAGATATTGTCCTCTTTGGGCTTTCCATTTCGGGCTTCCCTTCAAGGCTTTAAAACGCGTCTACTAGGGGAAGGTTTCCACACCCTTATAAAGGGTGTTTTGTTCTCCTCCCCAACCAACGTGGGAGGTCACAATCCACCTTCCTTTGGGGCCAGCGTCCTTGCTGGCACTCGTTCCTTCCTCCAATCGATGTGGGACCGTCACCAAATCCACCCCTTTTGGGGCCAGCAACCTTACTGGCACACTGCCTCGAGTCTACCCCCCTTCGGGGAACAGTGAGAAGGCTGACACATCGTCTGGTGTCTGCCTTTGATACTATTTGTAACGACCTAGGCCCACTGTAGCAAATTTTGTCCTCTTTGGGCTTTCCCTTTTGAGTTTTCCCTCAAGACTTTAAAACGCGTCTGTTAGGGGAAGGTTTCCACTCCTTTGTAAAGGGTGTTTTGTTCTCCTCCCCAACTAATGTGGGACATCACAGAAGCAATCTTTATAAGGGTGTGGAAACCTCTCCCTATCAGACATGTTTTAGAACCGTGAGGCTGGCGGAGATACGTAACGGACTAAAACGGACAATATCTGCTAACGACTAACGGTGACCTTGGGTTGTTACAAAATTAATGAAAAAATTTGGTAGTTCAATTTAGAAGCATTTTCTTTCAGCATGACATGGTGTTCTTCCCTTTTTAGATATCACTTTTTAGGGATAGATAGCACCCAAGTTTCTGAGTCTATAACTATATTACAGTATCTGAACAAAAAGTCAATACCTATGAACAAAGTTGTAGAACAGTTGAAGTTTGAGCTAGCTATTTTCGCTTCCCCGTTTGTTCTCGAATTGAATACGCGCCCATAACTATTTTCATGCTTTAATTTGTTTCATAGGACGAAACTAAGACGTGCAAATGTTCATTATCTTTCTCCTCCTGTCAAGGATGGGACAACAGGAACAGTCATTGTTCTTACTACCCCTGATGCTCAGCGAACAATGCTTGCGTATCAGGTAAACGTTTATTTAAACGGTTTTTTCCATATTCATATGTATCTGAAGTGGTAATTTTACAATCAGTTTTAGATGGCCCTTTTTGGTTCTCGATAGTCATAGCTGGTTGGTTCGTTTATCGAATTGGACTAACTTGAAGGGATTCTTCTGGATTTGCTATTTGATATCTCAGGGTACTTCTTCCATTGTGAATTATGGTCCAGCGTTAGCTAGTGTAATTAGCAAGACAAATGTACTTGTAGTTGAAGGGTATTTATTTGAATTTCCTGATACGATCAAAACGATTCAAAAAGCATGTGAGGAAGCACATCGAAGCGGTGCTCTAGTTGCAGTCACGGGTTCAGATGTCTCTTGCATCGAAAGACATTTTGACGATTTTTGGTAATTTTCTTCTCTGCCCATTTTTTATTTTGTTTTATTTACTTTGATAAAGAACAAAGGTTTCATTGAAAAAAATGAAAATCTTCAAAAGGGAACTCTACTAGACTCTACCGTGAGAACTAGAACGTGCGTTCGAAGTGTTGTACATTGTTTCTTTTTCATGCTAAGATGTTTTTTCGTTCATTCGTTCGATTTACTTCTCTTCAGGATTAAGCGAGTTGTTATTTAAAATATCTTTTCAATTTTATTATCAGGGAAATTGTGGGGAACTATGCTGATATTGTTTTCACAAACCATGAAGAAGCTGGAGCTTTATGTCATTTTGACTCCAAGGAAAGCTCTATATCAGCAGTAAGATATCTTAGTCATTTCGTTCCCCTCGTCTCGGTCACGGATGGGCACCGAGGTTCTTACTTAGGTGTTAAAGGAGAAGCAGTATATATCCCACCCTATCCATGCGTACCTCTCGATACTTGTGGTGCTGGAGATGCATATGCGTCTGGTATATTGTATGGTATCATGCACGGAGTCACGGATTTGAAACAAATGGGAACATTTGCAGCCAAAATCGCAGCCAGAGTCGTCGGGCAACAAGGAACTCGTTTAAGCGTTCAAGATGCCATGGAACTTGCAGATTCGTTTTCTTGCTTTCAGTCTGATATCGGTTCATCACACGCCTAGATTGTATCATGCAACTTGATCTTTAAGCTCATTTTCTTTATTAAGGGCTCAAGACTCCATGGAGATCGTTCAAGGGTTCAAGGTGCCATGGGAGATAGCATATTCATTTTCTTGCTTTCAGTCTGATATCTGAACTGTACAACTTGTTCTTTAAGCTCATTTTCTTTATTGAGTTCTCTCATCAAAGAAAATACATATGAAAGTGAAGATGTATGGAGATGTCTTGTAAGCCTCACATGTTAGTAAATGTGTATATTATTTCTTTTTTCCTATATA

mRNA sequence

AAAACTCTCGGAAGAAAAACAAGAATTAATATTGATCCATTTGTGGGGGAAGTAGGTTTATAAACTTAATTATCCGTTTCCCTACAGATGGGGGGAATGGGGCGGTGGGTGGCTCTGCTGAATTCCGCCATTTTTGCGTTTCTTTGATGCACCGATTCACCACCACTCGCTCCAAATCCCATTCGCTGCGATGTCTCTCTATTTTCCCATTTCTTCTCTTCCTCAATTTCACTTACCCTTTTCTTCTCAGCCTCTCTCTTATCCTAACTTGACTCCTCTTCATTCACTTCATTTTTCCTCTTCTTCATATCTCCCTTCTTCCTCTTCCACTTTCATTCACCGATGCACAGTTGGCGCCTGCTTCAGATTCTCTTCCATGGCCGGAGCCTTCGATTCTTTCACCGGTTTGTCTTCCTGTCGTGTTTTTAGCGGCGATGGGACAAGGGAGTTTTGTAGCGGGAGCGAGGAAGAAGAGGAGGAGGAGGGTGAGGGCAACGTCTCCGATGAAGAATTTGTGGTCGGAATTGAAGAATCTAATGACATAAATGGTTTGCCTGATCGATGGGATGTCTTGGGGCTTGGACAAGCTATGGTAGATTTCTCTGGTATGGTGGACGATGAGTTTCTGAAGAAGCTGGGACTAGAGAAGGGTACAAGGAAGCTTGTAAATCATGAGGAAAGAGGCAGAGTGTTGCGGGCGATGGATGGCCACAGCTATAAGGCGGCTGCTGGAGGGTCTCTTTCCAACAGCCTTGTGGCCTTGGCAAGGCTGGGTATTAGACCCATGAAAGGCCCTACTTTCAATGTAGCCATGACTGGTAGTGTAGGGAGTGATCCATTGGGCAGCTTCTACAGGACGAAACTAAGACGTGCAAATGTTCATTATCTTTCTCCTCCTGTCAAGGATGGGACAACAGGAACAGTCATTGTTCTTACTACCCCTGATGCTCAGCGAACAATGCTTGCGTATCAGGGTACTTCTTCCATTGTGAATTATGGTCCAGCGTTAGCTAGTGTAATTAGCAAGACAAATGTACTTGTAGTTGAAGGGTATTTATTTGAATTTCCTGATACGATCAAAACGATTCAAAAAGCATGTGAGGAAGCACATCGAAGCGGTGCTCTAGTTGCAGTCACGGGTTCAGATGTCTCTTGCATCGAAAGACATTTTGACGATTTTTGGGAAATTGTGGGGAACTATGCTGATATTGTTTTCACAAACCATGAAGAAGCTGGAGCTTTATGTCATTTTGACTCCAAGGAAAGCTCTATATCAGCAGTAAGATATCTTAGTCATTTCGTTCCCCTCGTCTCGGTCACGGATGGGCACCGAGGTTCTTACTTAGGTGTTAAAGGAGAAGCAGTATATATCCCACCCTATCCATGCGTACCTCTCGATACTTGTGGTGCTGGAGATGCATATGCGTCTGGTATATTGTATGGTATCATGCACGGAGTCACGGATTTGAAACAAATGGGAACATTTGCAGCCAAAATCGCAGCCAGAGTCGTCGGGCAACAAGGAACTCGTTTAAGCGTTCAAGATGCCATGGAACTTGCAGATTCGTTTTCTTGCTTTCAGTCTGATATCGGTTCATCACACGCCTAGATTGTATCATGCAACTTGATCTTTAAGCTCATTTTCTTTATTAAGGGCTCAAGACTCCATGGAGATCGTTCAAGGGTTCAAGGTGCCATGGGAGATAGCATATTCATTTTCTTGCTTTCAGTCTGATATCTGAACTGTACAACTTGTTCTTTAAGCTCATTTTCTTTATTGAGTTCTCTCATCAAAGAAAATACATATGAAAGTGAAGATGTATGGAGATGTCTTGTAAGCCTCACATGTTAGTAAATGTGTATATTATTTCTTTTTTCCTATATA

Coding sequence (CDS)

ATGTCTCTCTATTTTCCCATTTCTTCTCTTCCTCAATTTCACTTACCCTTTTCTTCTCAGCCTCTCTCTTATCCTAACTTGACTCCTCTTCATTCACTTCATTTTTCCTCTTCTTCATATCTCCCTTCTTCCTCTTCCACTTTCATTCACCGATGCACAGTTGGCGCCTGCTTCAGATTCTCTTCCATGGCCGGAGCCTTCGATTCTTTCACCGGTTTGTCTTCCTGTCGTGTTTTTAGCGGCGATGGGACAAGGGAGTTTTGTAGCGGGAGCGAGGAAGAAGAGGAGGAGGAGGGTGAGGGCAACGTCTCCGATGAAGAATTTGTGGTCGGAATTGAAGAATCTAATGACATAAATGGTTTGCCTGATCGATGGGATGTCTTGGGGCTTGGACAAGCTATGGTAGATTTCTCTGGTATGGTGGACGATGAGTTTCTGAAGAAGCTGGGACTAGAGAAGGGTACAAGGAAGCTTGTAAATCATGAGGAAAGAGGCAGAGTGTTGCGGGCGATGGATGGCCACAGCTATAAGGCGGCTGCTGGAGGGTCTCTTTCCAACAGCCTTGTGGCCTTGGCAAGGCTGGGTATTAGACCCATGAAAGGCCCTACTTTCAATGTAGCCATGACTGGTAGTGTAGGGAGTGATCCATTGGGCAGCTTCTACAGGACGAAACTAAGACGTGCAAATGTTCATTATCTTTCTCCTCCTGTCAAGGATGGGACAACAGGAACAGTCATTGTTCTTACTACCCCTGATGCTCAGCGAACAATGCTTGCGTATCAGGGTACTTCTTCCATTGTGAATTATGGTCCAGCGTTAGCTAGTGTAATTAGCAAGACAAATGTACTTGTAGTTGAAGGGTATTTATTTGAATTTCCTGATACGATCAAAACGATTCAAAAAGCATGTGAGGAAGCACATCGAAGCGGTGCTCTAGTTGCAGTCACGGGTTCAGATGTCTCTTGCATCGAAAGACATTTTGACGATTTTTGGGAAATTGTGGGGAACTATGCTGATATTGTTTTCACAAACCATGAAGAAGCTGGAGCTTTATGTCATTTTGACTCCAAGGAAAGCTCTATATCAGCAGTAAGATATCTTAGTCATTTCGTTCCCCTCGTCTCGGTCACGGATGGGCACCGAGGTTCTTACTTAGGTGTTAAAGGAGAAGCAGTATATATCCCACCCTATCCATGCGTACCTCTCGATACTTGTGGTGCTGGAGATGCATATGCGTCTGGTATATTGTATGGTATCATGCACGGAGTCACGGATTTGAAACAAATGGGAACATTTGCAGCCAAAATCGCAGCCAGAGTCGTCGGGCAACAAGGAACTCGTTTAAGCGTTCAAGATGCCATGGAACTTGCAGATTCGTTTTCTTGCTTTCAGTCTGATATCGGTTCATCACACGCCTAG

Protein sequence

MSLYFPISSLPQFHLPFSSQPLSYPNLTPLHSLHFSSSSYLPSSSSTFIHRCTVGACFRFSSMAGAFDSFTGLSSCRVFSGDGTREFCSGSEEEEEEEGEGNVSDEEFVVGIEESNDINGLPDRWDVLGLGQAMVDFSGMVDDEFLKKLGLEKGTRKLVNHEERGRVLRAMDGHSYKAAAGGSLSNSLVALARLGIRPMKGPTFNVAMTGSVGSDPLGSFYRTKLRRANVHYLSPPVKDGTTGTVIVLTTPDAQRTMLAYQGTSSIVNYGPALASVISKTNVLVVEGYLFEFPDTIKTIQKACEEAHRSGALVAVTGSDVSCIERHFDDFWEIVGNYADIVFTNHEEAGALCHFDSKESSISAVRYLSHFVPLVSVTDGHRGSYLGVKGEAVYIPPYPCVPLDTCGAGDAYASGILYGIMHGVTDLKQMGTFAAKIAARVVGQQGTRLSVQDAMELADSFSCFQSDIGSSHA
Homology
BLAST of Carg08420 vs. NCBI nr
Match: KAG7035625.1 (hypothetical protein SDJN02_02423 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 935.3 bits (2416), Expect = 2.1e-268
Identity = 472/472 (100.00%), Postives = 472/472 (100.00%), Query Frame = 0

Query: 1   MSLYFPISSLPQFHLPFSSQPLSYPNLTPLHSLHFSSSSYLPSSSSTFIHRCTVGACFRF 60
           MSLYFPISSLPQFHLPFSSQPLSYPNLTPLHSLHFSSSSYLPSSSSTFIHRCTVGACFRF
Sbjct: 1   MSLYFPISSLPQFHLPFSSQPLSYPNLTPLHSLHFSSSSYLPSSSSTFIHRCTVGACFRF 60

Query: 61  SSMAGAFDSFTGLSSCRVFSGDGTREFCSGSEEEEEEEGEGNVSDEEFVVGIEESNDING 120
           SSMAGAFDSFTGLSSCRVFSGDGTREFCSGSEEEEEEEGEGNVSDEEFVVGIEESNDING
Sbjct: 61  SSMAGAFDSFTGLSSCRVFSGDGTREFCSGSEEEEEEEGEGNVSDEEFVVGIEESNDING 120

Query: 121 LPDRWDVLGLGQAMVDFSGMVDDEFLKKLGLEKGTRKLVNHEERGRVLRAMDGHSYKAAA 180
           LPDRWDVLGLGQAMVDFSGMVDDEFLKKLGLEKGTRKLVNHEERGRVLRAMDGHSYKAAA
Sbjct: 121 LPDRWDVLGLGQAMVDFSGMVDDEFLKKLGLEKGTRKLVNHEERGRVLRAMDGHSYKAAA 180

Query: 181 GGSLSNSLVALARLGIRPMKGPTFNVAMTGSVGSDPLGSFYRTKLRRANVHYLSPPVKDG 240
           GGSLSNSLVALARLGIRPMKGPTFNVAMTGSVGSDPLGSFYRTKLRRANVHYLSPPVKDG
Sbjct: 181 GGSLSNSLVALARLGIRPMKGPTFNVAMTGSVGSDPLGSFYRTKLRRANVHYLSPPVKDG 240

Query: 241 TTGTVIVLTTPDAQRTMLAYQGTSSIVNYGPALASVISKTNVLVVEGYLFEFPDTIKTIQ 300
           TTGTVIVLTTPDAQRTMLAYQGTSSIVNYGPALASVISKTNVLVVEGYLFEFPDTIKTIQ
Sbjct: 241 TTGTVIVLTTPDAQRTMLAYQGTSSIVNYGPALASVISKTNVLVVEGYLFEFPDTIKTIQ 300

Query: 301 KACEEAHRSGALVAVTGSDVSCIERHFDDFWEIVGNYADIVFTNHEEAGALCHFDSKESS 360
           KACEEAHRSGALVAVTGSDVSCIERHFDDFWEIVGNYADIVFTNHEEAGALCHFDSKESS
Sbjct: 301 KACEEAHRSGALVAVTGSDVSCIERHFDDFWEIVGNYADIVFTNHEEAGALCHFDSKESS 360

Query: 361 ISAVRYLSHFVPLVSVTDGHRGSYLGVKGEAVYIPPYPCVPLDTCGAGDAYASGILYGIM 420
           ISAVRYLSHFVPLVSVTDGHRGSYLGVKGEAVYIPPYPCVPLDTCGAGDAYASGILYGIM
Sbjct: 361 ISAVRYLSHFVPLVSVTDGHRGSYLGVKGEAVYIPPYPCVPLDTCGAGDAYASGILYGIM 420

Query: 421 HGVTDLKQMGTFAAKIAARVVGQQGTRLSVQDAMELADSFSCFQSDIGSSHA 473
           HGVTDLKQMGTFAAKIAARVVGQQGTRLSVQDAMELADSFSCFQSDIGSSHA
Sbjct: 421 HGVTDLKQMGTFAAKIAARVVGQQGTRLSVQDAMELADSFSCFQSDIGSSHA 472

BLAST of Carg08420 vs. NCBI nr
Match: XP_022958117.1 (uncharacterized protein LOC111459440 [Cucurbita moschata])

HSP 1 Score: 925.6 bits (2391), Expect = 1.7e-265
Identity = 470/473 (99.37%), Postives = 470/473 (99.37%), Query Frame = 0

Query: 1   MSLYFPISSLPQFHLPFSSQPLSYPNLTPLHSLHFSSSSYLPSSSSTFIHRCTVGACFRF 60
           MSLYFPISSLPQFHLPFSSQPLSYPNLTPLHSLHFSSSSYLPSSSSTFIHRCTVGACFRF
Sbjct: 1   MSLYFPISSLPQFHLPFSSQPLSYPNLTPLHSLHFSSSSYLPSSSSTFIHRCTVGACFRF 60

Query: 61  SSMAGAFDSFTGLSSCRVFSGDGTREFCSGS-EEEEEEEGEGNVSDEEFVVGIEESNDIN 120
           SSMAGAFDS TGLSSCRVFSGDGTREFCSGS EEEEEEE EGNVSDEEFVVGIEESNDIN
Sbjct: 61  SSMAGAFDSLTGLSSCRVFSGDGTREFCSGSEEEEEEEEDEGNVSDEEFVVGIEESNDIN 120

Query: 121 GLPDRWDVLGLGQAMVDFSGMVDDEFLKKLGLEKGTRKLVNHEERGRVLRAMDGHSYKAA 180
           GLPDRWDVLGLGQAMVDFSGMVDDEFLKKLGLEKGTRKLVNHEERGRVLRAMDGHSYKAA
Sbjct: 121 GLPDRWDVLGLGQAMVDFSGMVDDEFLKKLGLEKGTRKLVNHEERGRVLRAMDGHSYKAA 180

Query: 181 AGGSLSNSLVALARLGIRPMKGPTFNVAMTGSVGSDPLGSFYRTKLRRANVHYLSPPVKD 240
           AGGSLSNSLVALARLGIRPMKGPTFNVAMTGSVGSDPLGSFYRTKLRRANVHYLSPPVKD
Sbjct: 181 AGGSLSNSLVALARLGIRPMKGPTFNVAMTGSVGSDPLGSFYRTKLRRANVHYLSPPVKD 240

Query: 241 GTTGTVIVLTTPDAQRTMLAYQGTSSIVNYGPALASVISKTNVLVVEGYLFEFPDTIKTI 300
           GTTGTVIVLTTPDAQRTMLAYQGTSSIVNYGPALASVISKTNVLVVEGYLFEFPDTIKTI
Sbjct: 241 GTTGTVIVLTTPDAQRTMLAYQGTSSIVNYGPALASVISKTNVLVVEGYLFEFPDTIKTI 300

Query: 301 QKACEEAHRSGALVAVTGSDVSCIERHFDDFWEIVGNYADIVFTNHEEAGALCHFDSKES 360
           QKACEEAHRSGALVAVTGSDVSCIERHFDDFWEIVGNYADIVFTNHEEAGALCHFDSKES
Sbjct: 301 QKACEEAHRSGALVAVTGSDVSCIERHFDDFWEIVGNYADIVFTNHEEAGALCHFDSKES 360

Query: 361 SISAVRYLSHFVPLVSVTDGHRGSYLGVKGEAVYIPPYPCVPLDTCGAGDAYASGILYGI 420
           SISAVRYLSHFVPLVSVTDGHRGSYLGVKGEAVYIPPYPCVPLDTCGAGDAYASGILYGI
Sbjct: 361 SISAVRYLSHFVPLVSVTDGHRGSYLGVKGEAVYIPPYPCVPLDTCGAGDAYASGILYGI 420

Query: 421 MHGVTDLKQMGTFAAKIAARVVGQQGTRLSVQDAMELADSFSCFQSDIGSSHA 473
           MHGVTDLKQMGTFAAKIAARVVGQQGTRLSVQDAMELADSFSCFQSDIGSSHA
Sbjct: 421 MHGVTDLKQMGTFAAKIAARVVGQQGTRLSVQDAMELADSFSCFQSDIGSSHA 473

BLAST of Carg08420 vs. NCBI nr
Match: XP_023534341.1 (uncharacterized protein LOC111795932 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 918.7 bits (2373), Expect = 2.1e-263
Identity = 467/474 (98.52%), Postives = 469/474 (98.95%), Query Frame = 0

Query: 1   MSLYFPISSLPQFHLPFSSQPLSYPNLTPLHSLHFSSSSYLPSSSSTFIHRCTVGACFRF 60
           MSLYFPISSLPQFHLP SSQPLSYPNLTPLHSLHFSSSSYLPSSSSTFIH CTVGACFRF
Sbjct: 1   MSLYFPISSLPQFHLPSSSQPLSYPNLTPLHSLHFSSSSYLPSSSSTFIHPCTVGACFRF 60

Query: 61  SSMAGAFDSFTGLSSCRVFSGDGTREFCSGS--EEEEEEEGEGNVSDEEFVVGIEESNDI 120
           SSMAGAFDSFTGLSSCRVFSGDGTREFCSGS  EEEEEEE EGNVSDEEFVVGIEESN+I
Sbjct: 61  SSMAGAFDSFTGLSSCRVFSGDGTREFCSGSEEEEEEEEEDEGNVSDEEFVVGIEESNEI 120

Query: 121 NGLPDRWDVLGLGQAMVDFSGMVDDEFLKKLGLEKGTRKLVNHEERGRVLRAMDGHSYKA 180
           NGLPDRWDVLGLGQAMVDFSGMVDDEFLKKLGLEKGTRKLVNHEERGRVLRAMDGHSYKA
Sbjct: 121 NGLPDRWDVLGLGQAMVDFSGMVDDEFLKKLGLEKGTRKLVNHEERGRVLRAMDGHSYKA 180

Query: 181 AAGGSLSNSLVALARLGIRPMKGPTFNVAMTGSVGSDPLGSFYRTKLRRANVHYLSPPVK 240
           AAGGSLSNSLVALARLGIRPMKGPTFNVAMTGSVGSDPLGSFYRTKLRRANVHYLSPPVK
Sbjct: 181 AAGGSLSNSLVALARLGIRPMKGPTFNVAMTGSVGSDPLGSFYRTKLRRANVHYLSPPVK 240

Query: 241 DGTTGTVIVLTTPDAQRTMLAYQGTSSIVNYGPALASVISKTNVLVVEGYLFEFPDTIKT 300
           DGTTGTVIVLTTPDAQRTMLAYQGTSSIVNYGPALASVISKTNVLVVEGYLFEFPDTIKT
Sbjct: 241 DGTTGTVIVLTTPDAQRTMLAYQGTSSIVNYGPALASVISKTNVLVVEGYLFEFPDTIKT 300

Query: 301 IQKACEEAHRSGALVAVTGSDVSCIERHFDDFWEIVGNYADIVFTNHEEAGALCHFDSKE 360
           IQKACEEAHRSGALVAVTGSDVSCIERHFDDFWEIVGNYADIVFTNHEEAGALCHFDSKE
Sbjct: 301 IQKACEEAHRSGALVAVTGSDVSCIERHFDDFWEIVGNYADIVFTNHEEAGALCHFDSKE 360

Query: 361 SSISAVRYLSHFVPLVSVTDGHRGSYLGVKGEAVYIPPYPCVPLDTCGAGDAYASGILYG 420
           SSISAVRYLSHFVPLVSVTDGHRGSYLGVKGEAVYIPPYPCVPLDTCGAGDAYASGILYG
Sbjct: 361 SSISAVRYLSHFVPLVSVTDGHRGSYLGVKGEAVYIPPYPCVPLDTCGAGDAYASGILYG 420

Query: 421 IMHGVTDLKQMGTFAAKIAARVVGQQGTRLSVQDAMELADSFSCFQSDIGSSHA 473
           IMHGV+DLKQMGTFAAKIAARVVGQQGTRLSVQDAMELADSFSCFQSDIGSSHA
Sbjct: 421 IMHGVSDLKQMGTFAAKIAARVVGQQGTRLSVQDAMELADSFSCFQSDIGSSHA 474

BLAST of Carg08420 vs. NCBI nr
Match: XP_022995190.1 (uncharacterized protein LOC111490810 [Cucurbita maxima])

HSP 1 Score: 914.4 bits (2362), Expect = 3.9e-262
Identity = 465/474 (98.10%), Postives = 467/474 (98.52%), Query Frame = 0

Query: 1   MSLYFPISSLPQFHLPFSSQPLSYPNLTPLHSLHFSSSSYLPSSSSTFIHRCTVGACFRF 60
           MSLYFPISSLPQFHLPFSSQPLSYPNLTPLHSLHFSSSSYLPSSSSTFIHRC V ACFRF
Sbjct: 1   MSLYFPISSLPQFHLPFSSQPLSYPNLTPLHSLHFSSSSYLPSSSSTFIHRCRVVACFRF 60

Query: 61  SSMAGAFDSFTGLSSCRVFSGDGTREFCSGSEEE--EEEEGEGNVSDEEFVVGIEESNDI 120
           SSMAG FDSFTGLSSCRVFSGDGTREFCSGSEEE  EEEE EGNVSDEEF VGIEESN+I
Sbjct: 61  SSMAGGFDSFTGLSSCRVFSGDGTREFCSGSEEEEQEEEEDEGNVSDEEFGVGIEESNEI 120

Query: 121 NGLPDRWDVLGLGQAMVDFSGMVDDEFLKKLGLEKGTRKLVNHEERGRVLRAMDGHSYKA 180
           NGLPDRWDVLGLGQAMVDFSGMVDDEFLKKLGLEKGTRKLVNHEERGRVLRAMDGHSYKA
Sbjct: 121 NGLPDRWDVLGLGQAMVDFSGMVDDEFLKKLGLEKGTRKLVNHEERGRVLRAMDGHSYKA 180

Query: 181 AAGGSLSNSLVALARLGIRPMKGPTFNVAMTGSVGSDPLGSFYRTKLRRANVHYLSPPVK 240
           AAGGSLSNSLVALARLGIRPMKGPTFNVAMTGSVGSDPLGSFYRTKLRRANVHYLSPPVK
Sbjct: 181 AAGGSLSNSLVALARLGIRPMKGPTFNVAMTGSVGSDPLGSFYRTKLRRANVHYLSPPVK 240

Query: 241 DGTTGTVIVLTTPDAQRTMLAYQGTSSIVNYGPALASVISKTNVLVVEGYLFEFPDTIKT 300
           DGTTGTVIVLTTPDAQRTMLAYQGTSSIVNYGPALASVISKTNVLVVEGYLFEFPDTIKT
Sbjct: 241 DGTTGTVIVLTTPDAQRTMLAYQGTSSIVNYGPALASVISKTNVLVVEGYLFEFPDTIKT 300

Query: 301 IQKACEEAHRSGALVAVTGSDVSCIERHFDDFWEIVGNYADIVFTNHEEAGALCHFDSKE 360
           IQKACEEAHRSGALVAVTGSDVSCIERHFDDFWEIVGNYADIVFTNHEEAGALCHFDSKE
Sbjct: 301 IQKACEEAHRSGALVAVTGSDVSCIERHFDDFWEIVGNYADIVFTNHEEAGALCHFDSKE 360

Query: 361 SSISAVRYLSHFVPLVSVTDGHRGSYLGVKGEAVYIPPYPCVPLDTCGAGDAYASGILYG 420
           SSISAVRYLSHFVPLVSVTDGHRGSYLGVKGEAVYIPPYPCVPLDTCGAGDAYASGILYG
Sbjct: 361 SSISAVRYLSHFVPLVSVTDGHRGSYLGVKGEAVYIPPYPCVPLDTCGAGDAYASGILYG 420

Query: 421 IMHGVTDLKQMGTFAAKIAARVVGQQGTRLSVQDAMELADSFSCFQSDIGSSHA 473
           IMHGV+DLKQMGTFAAKIAARVVGQQGTRLSVQDAMELADSFSCFQSDIGSSHA
Sbjct: 421 IMHGVSDLKQMGTFAAKIAARVVGQQGTRLSVQDAMELADSFSCFQSDIGSSHA 474

BLAST of Carg08420 vs. NCBI nr
Match: XP_038888543.1 (uncharacterized sugar kinase slr0537 [Benincasa hispida])

HSP 1 Score: 795.4 bits (2053), Expect = 2.6e-226
Identity = 415/477 (87.00%), Postives = 435/477 (91.19%), Query Frame = 0

Query: 1   MSLYFPISSLPQFHLPFSSQPLSYPNLTPLHSLHF-SSSSYLPSSSSTFIHRCTVGACFR 60
           MSLYFPISSLPQF LPFSS PLS PN TP+ SL F SSSSY PSSS TFIHRC      R
Sbjct: 1   MSLYFPISSLPQFQLPFSSLPLSSPNFTPIPSLPFPSSSSYSPSSSFTFIHRC------R 60

Query: 61  FSSMAGAFDSFTGLSSCRVFSGDGTREFCSGSEEEEEEEGEGNVSDEEFVVGIEESNDIN 120
           FSSMAG FDSFT L SCRVFS  GT EF SGSEEEE+++ EGN SDEEFVVGI+++++IN
Sbjct: 61  FSSMAGGFDSFTPLCSCRVFSSYGTGEFSSGSEEEEDDD-EGNGSDEEFVVGIDDADEIN 120

Query: 121 -----GLPDRWDVLGLGQAMVDFSGMVDDEFLKKLGLEKGTRKLVNHEERGRVLRAMDGH 180
                 LPDRWDVLGLGQAMVDFSGMVDDEFLKKLGLEKGTRK+VNHEERGRVLRAMDG 
Sbjct: 121 PSSSSRLPDRWDVLGLGQAMVDFSGMVDDEFLKKLGLEKGTRKVVNHEERGRVLRAMDGR 180

Query: 181 SYKAAAGGSLSNSLVALARLGIRPMKGPTFNVAMTGSVGSDPLGSFYRTKLRRANVHYLS 240
           SYKAAAGGSLSNSLVALARLGIRPMKGPTFNVAMTGS+GSDPLGSFYR+KLRRANVH+LS
Sbjct: 181 SYKAAAGGSLSNSLVALARLGIRPMKGPTFNVAMTGSIGSDPLGSFYRSKLRRANVHFLS 240

Query: 241 PPVKDGTTGTVIVLTTPDAQRTMLAYQGTSSIVNYGPALASVISKTNVLVVEGYLFEFPD 300
           PPVKDGTTGTVIVLTTPDAQRTMLAYQGTSS VNY P+LASVISKTNVLVVEGYLFE PD
Sbjct: 241 PPVKDGTTGTVIVLTTPDAQRTMLAYQGTSSTVNYSPSLASVISKTNVLVVEGYLFELPD 300

Query: 301 TIKTIQKACEEAHRSGALVAVTGSDVSCIERHFDDFWEIVGNYADIVFTNHEEAGALCHF 360
           TIKTIQKACEEAHRSGALVAVT SDVSCI RHFDDFWEIVGNYADIVF NHEEA ALCHF
Sbjct: 301 TIKTIQKACEEAHRSGALVAVTASDVSCIARHFDDFWEIVGNYADIVFANHEEAAALCHF 360

Query: 361 DSKESSISAVRYLSHFVPLVSVTDGHRGSYLGVKGEAVYIPPYPCVPLDTCGAGDAYASG 420
           DSKESS+SAVRYLSHFVPLVSVTDGHRGSY+GVKGEAVYIPP PCVPLDTCGAGDAYASG
Sbjct: 361 DSKESSVSAVRYLSHFVPLVSVTDGHRGSYIGVKGEAVYIPPCPCVPLDTCGAGDAYASG 420

Query: 421 ILYGIMHGVTDLKQMGTFAAKIAARVVGQQGTRLSVQDAMELADSFSCFQSDIGSSH 472
           ILYGI+ GV+DLK+MGTFAAKIAARVVGQQGTRL VQDAMELAD+FS FQSDIGSSH
Sbjct: 421 ILYGILQGVSDLKEMGTFAAKIAARVVGQQGTRLRVQDAMELADAFSSFQSDIGSSH 470

BLAST of Carg08420 vs. ExPASy Swiss-Prot
Match: Q55480 (Uncharacterized sugar kinase slr0537 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=slr0537 PE=3 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 3.8e-34
Identity = 110/341 (32.26%), Postives = 162/341 (47.51%), Query Frame = 0

Query: 124 RWDVLGLGQAMVDFSGMVDDEFLKKLGLEKGTRKLVNHEERGRVLRAMDGHSYKAAAGGS 183
           ++DV G+G A+VD    V  E L  LG++KG   LV       ++  +     K ++GGS
Sbjct: 4   KYDVYGMGNALVDMEFEVTPEQLASLGIDKGVMTLVEEARENELIAQLAQQRGKQSSGGS 63

Query: 184 LSNSLVALARLGIRPMKGPTFNVAMTGSVGSDPLGSFYRTKLR----RANVHYLSPPVKD 243
            +N+LV+LA+LG     G  F       VG D  G+FY   L       N H+      +
Sbjct: 64  AANTLVSLAQLG-----GTGFYAC---KVGKDEAGAFYLQDLNDCGLDTNPHH--ETAGE 123

Query: 244 GTTGTVIVLTTPDAQRTMLAYQGTSSIVNYGPALASVISKTNVLVVEGYLFEFPDTIKTI 303
           G TG  +V  TPDA RTM A+ G S  ++      S + ++  L +EGYL   P      
Sbjct: 124 GITGKCLVFVTPDADRTMNAFLGISGSLSVTEMDWSALKQSQYLYLEGYLVTSPSAKAAC 183

Query: 304 QKACEEAHRSGALVAVTGSDVSCIERHFDDFWEIVGNYADIVFTNHEEAGALCHFDSKES 363
            +A   A +SG    ++ SD +  +   D   E++G+  D++F N  EA           
Sbjct: 184 IEAKAIAEQSGVKTCLSLSDPNMAKFFQDGLKEMLGSGVDLLFANEAEA---LEMAGTSD 243

Query: 364 SISAVRYLSHFVPLVSVTDGHRGSYLGVKGEAVYIPPYPCV-PLDTCGAGDAYASGILYG 423
              A+ Y        ++T G  GS +   GE +     P V P+DT GAGD YA G LYG
Sbjct: 244 LNQAIAYCKSIAKNFALTRGGAGSLI-FDGENLLTIGTPKVQPIDTVGAGDMYAGGFLYG 303

Query: 424 IMHGVTDLKQMGTFAAKIAARVVGQQGTRLSVQDAMELADS 460
           + HG+ D ++ G  A++ AA+VV   G RL  +   E+  S
Sbjct: 304 LTHGM-DYEKAGQLASETAAKVVTCYGPRLDTEILQEILQS 329

BLAST of Carg08420 vs. ExPASy Swiss-Prot
Match: A6M229 (5-dehydro-2-deoxygluconokinase OS=Clostridium beijerinckii (strain ATCC 51743 / NCIMB 8052) OX=290402 GN=iolC PE=3 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 2.6e-11
Identity = 69/278 (24.82%), Postives = 118/278 (42.45%), Query Frame = 0

Query: 172 DGHSYKAAAGGSLSNSLVALARLGIRPMKGPTFNVAMTGSVGSDPLGSFY-----RTKLR 231
           +  ++K   GGS +N  V LARLG +        +   G V  D  G F         + 
Sbjct: 35  ESSTFKKYLGGSPANIAVGLARLGKK--------IGFIGKVSKDQFGEFVVNYFDNEGID 94

Query: 232 RANVHYLSPPVKDGTTGTVIVLTTPDAQRTMLAYQGTSSI-VNYGPALASVISKTNVLVV 291
            + + Y       G T T I   +P     ++  QG + + +N        I  T  +V+
Sbjct: 95  TSQIKYSKNGENLGLTFTEI--ASPTESSILMYRQGIADLELNVDEIDEEYIKNTKAIVI 154

Query: 292 EGYLFEFPDTIKTIQKACEEAHRSGALVAVTGSDVSCIERHFDD---FWEIVGNYADIVF 351
            G       + +   KA E A R+  +V       +   ++ D+   ++ I G  +DI+ 
Sbjct: 155 SGTALAKSPSREAALKALELAKRNNTVVIFDVDYRAYNWKNSDEIAIYYSIAGKQSDIIM 214

Query: 352 TNHEEAGALCHFDSKESSI---SAVRYLSHFVPLVSVTDGHRGSYLGVK-GEAVYIPPYP 411
            + EE   +    ++E +    +A R+L +   +V +  G  GS      G++  I P+P
Sbjct: 215 GSREEFDLMEKLITREKNSDEETAKRWLDYGNKIVVIKHGKEGSTAYTSDGKSYNIKPFP 274

Query: 412 CVPLDTCGAGDAYASGILYGIMHG--VTDLKQMGTFAA 435
              L + G GDAYAS  LYG++ G  + D  + G+ +A
Sbjct: 275 VKLLKSFGGGDAYASAFLYGLLEGWSIIDSLEFGSASA 302

BLAST of Carg08420 vs. ExPASy Swiss-Prot
Match: Q0TUZ4 (5-dehydro-2-deoxygluconokinase OS=Clostridium perfringens (strain ATCC 13124 / DSM 756 / JCM 1290 / NCIMB 6125 / NCTC 8237 / Type A) OX=195103 GN=iolC PE=3 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 3.4e-11
Identity = 75/290 (25.86%), Postives = 122/290 (42.07%), Query Frame = 0

Query: 172 DGHSYKAAAGGSLSNSLVALARLGIRPMKGPTFNVAMTGSVGSDPLGSFYRTKLRRANVH 231
           +  ++    GGS +N  V LARLG +        V   G V  D  G F     ++  + 
Sbjct: 35  ESRNFNKYLGGSPANIAVGLARLGKK--------VGFIGKVSDDRFGEFVVNYFKKEGID 94

Query: 232 YLSPPVKDGTTGTVIVLTTPDAQRTMLAYQGTSSIVNYGPALASV-----------ISKT 291
                +     G  + LT      T +     SSI+ Y   +A +           I  T
Sbjct: 95  V--SEISKAKNGESLGLTF-----TEILSPTESSILMYRNGIADLQLDVDDIDEDYIKNT 154

Query: 292 NVLVVEGYLFEFPDTIKTIQKACEEAHRSGALVAVTGSDVSCIERHFDD------FWEIV 351
             +V+ G       + +   KA   A ++G +V     DV   E ++ +      ++ IV
Sbjct: 155 KAIVISGTALAMSPSREAALKALRLAKKNGTVVIF---DVDYREYNWKNKDEIAIYYSIV 214

Query: 352 GNYADIVFTNHEEAGALCHFDSKESS--ISAVRYLSHFVPLVSVTDGHRGSYLGVK-GEA 411
           G  +DI+  + EE   +    +K+S+   +A R+L +   +V +  G  GS    + G+A
Sbjct: 215 GKESDIIMGSREEFDLMEGLIAKDSTDEETAKRWLDYGNKIVVIKHGKDGSTAYTRDGKA 274

Query: 412 VYIPPYPCVPLDTCGAGDAYASGILYGIMHGVTDLKQMGTFAAKIAARVV 442
             I P+P   L + G GDAYAS  +YGI+ G  D+     F +  AA +V
Sbjct: 275 YRIKPFPVKLLKSFGGGDAYASAFIYGILEG-WDMMDALEFGSASAAMLV 305

BLAST of Carg08420 vs. ExPASy Swiss-Prot
Match: Q8XP78 (5-dehydro-2-deoxygluconokinase OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=iolC PE=3 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 3.4e-11
Identity = 75/290 (25.86%), Postives = 122/290 (42.07%), Query Frame = 0

Query: 172 DGHSYKAAAGGSLSNSLVALARLGIRPMKGPTFNVAMTGSVGSDPLGSFYRTKLRRANVH 231
           +  ++    GGS +N  V LARLG +        V   G V  D  G F     ++  + 
Sbjct: 35  ESRNFNKYLGGSPANIAVGLARLGKK--------VGFIGKVSDDRFGEFVVNYFKKEGID 94

Query: 232 YLSPPVKDGTTGTVIVLTTPDAQRTMLAYQGTSSIVNYGPALASV-----------ISKT 291
                +     G  + LT      T +     SSI+ Y   +A +           I  T
Sbjct: 95  V--SEISKAKNGESLGLTF-----TEILSPTESSILMYRNGIADLQLDVDDIDEDYIKNT 154

Query: 292 NVLVVEGYLFEFPDTIKTIQKACEEAHRSGALVAVTGSDVSCIERHFDD------FWEIV 351
             +V+ G       + +   KA   A ++G +V     DV   E ++ +      ++ IV
Sbjct: 155 KAIVISGTALAMSPSREAALKALRLAKKNGTVVIF---DVDYREYNWKNKDEIAIYYSIV 214

Query: 352 GNYADIVFTNHEEAGALCHFDSKESS--ISAVRYLSHFVPLVSVTDGHRGSYLGVK-GEA 411
           G  +DI+  + EE   +    +K+S+   +A R+L +   +V +  G  GS    + G+A
Sbjct: 215 GKESDIIMGSREEFDLMEGLIAKDSTDEETAKRWLDYGNKIVVIKHGKDGSTAYTRDGKA 274

Query: 412 VYIPPYPCVPLDTCGAGDAYASGILYGIMHGVTDLKQMGTFAAKIAARVV 442
             I P+P   L + G GDAYAS  +YGI+ G  D+     F +  AA +V
Sbjct: 275 YRIKPFPVKLLKSFGGGDAYASAFIYGILEG-WDMMDALEFGSASAAMLV 305

BLAST of Carg08420 vs. ExPASy Swiss-Prot
Match: B2V4J8 (5-dehydro-2-deoxygluconokinase OS=Clostridium botulinum (strain Alaska E43 / Type E3) OX=508767 GN=iolC PE=3 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 1.3e-10
Identity = 76/283 (26.86%), Postives = 117/283 (41.34%), Query Frame = 0

Query: 175 SYKAAAGGSLSNSLVALARLGIRPMKGPTFNVAMTGSVGSDPLGSFYRTKLRRANVHYLS 234
           ++K   GGS +N  V L+RLG +        V   G V  D  G F         +   +
Sbjct: 38  TFKKYLGGSPANIAVGLSRLGKK--------VGFIGKVSKDQFGKFVVDYFNNEGID--T 97

Query: 235 PPVKDGTTGTVIVLTTPDAQRTMLAYQGTSSIVNYGPALASV-----------ISKTNVL 294
             +K    G  + LT      T +A    SSI+ Y   +A +           I  T  +
Sbjct: 98  SQIKYAENGESLGLTF-----TEIASPTESSILMYRNGIADLELDVNEIDEEYIKNTKAI 157

Query: 295 VVEGYLFEFPDTIKTIQKACEEAHRSGALVAVTGSDVSCIERHFDD------FWEIVGNY 354
           V+ G       + +   KA E A ++  +V     DV   E ++ +      ++ IVG  
Sbjct: 158 VISGTALAKSPSREAALKALELAKKNDTVVIF---DVDYREYNWKNKDEIAIYYSIVGKQ 217

Query: 355 ADIVFTNHEEAGALCHFDSKESSI---SAVRYLSHFVPLVSVTDGHRGSYLGVK-GEAVY 414
           +DIV  + EE   +     KE S    SA R+L     +V +  G  GS      G++  
Sbjct: 218 SDIVMGSREEFDLMESLIVKEKSTDEESAKRWLGFGNKIVVIKHGKEGSTAYTNDGKSYK 277

Query: 415 IPPYPCVPLDTCGAGDAYASGILYGIMH--GVTDLKQMGTFAA 435
           I P+P   L + G GDAYAS  +YGI+    + D  + G+ +A
Sbjct: 278 IKPFPVKLLKSFGGGDAYASAFIYGILEEWDIMDALEFGSASA 302

BLAST of Carg08420 vs. ExPASy TrEMBL
Match: A0A6J1H108 (uncharacterized protein LOC111459440 OS=Cucurbita moschata OX=3662 GN=LOC111459440 PE=3 SV=1)

HSP 1 Score: 925.6 bits (2391), Expect = 8.1e-266
Identity = 470/473 (99.37%), Postives = 470/473 (99.37%), Query Frame = 0

Query: 1   MSLYFPISSLPQFHLPFSSQPLSYPNLTPLHSLHFSSSSYLPSSSSTFIHRCTVGACFRF 60
           MSLYFPISSLPQFHLPFSSQPLSYPNLTPLHSLHFSSSSYLPSSSSTFIHRCTVGACFRF
Sbjct: 1   MSLYFPISSLPQFHLPFSSQPLSYPNLTPLHSLHFSSSSYLPSSSSTFIHRCTVGACFRF 60

Query: 61  SSMAGAFDSFTGLSSCRVFSGDGTREFCSGS-EEEEEEEGEGNVSDEEFVVGIEESNDIN 120
           SSMAGAFDS TGLSSCRVFSGDGTREFCSGS EEEEEEE EGNVSDEEFVVGIEESNDIN
Sbjct: 61  SSMAGAFDSLTGLSSCRVFSGDGTREFCSGSEEEEEEEEDEGNVSDEEFVVGIEESNDIN 120

Query: 121 GLPDRWDVLGLGQAMVDFSGMVDDEFLKKLGLEKGTRKLVNHEERGRVLRAMDGHSYKAA 180
           GLPDRWDVLGLGQAMVDFSGMVDDEFLKKLGLEKGTRKLVNHEERGRVLRAMDGHSYKAA
Sbjct: 121 GLPDRWDVLGLGQAMVDFSGMVDDEFLKKLGLEKGTRKLVNHEERGRVLRAMDGHSYKAA 180

Query: 181 AGGSLSNSLVALARLGIRPMKGPTFNVAMTGSVGSDPLGSFYRTKLRRANVHYLSPPVKD 240
           AGGSLSNSLVALARLGIRPMKGPTFNVAMTGSVGSDPLGSFYRTKLRRANVHYLSPPVKD
Sbjct: 181 AGGSLSNSLVALARLGIRPMKGPTFNVAMTGSVGSDPLGSFYRTKLRRANVHYLSPPVKD 240

Query: 241 GTTGTVIVLTTPDAQRTMLAYQGTSSIVNYGPALASVISKTNVLVVEGYLFEFPDTIKTI 300
           GTTGTVIVLTTPDAQRTMLAYQGTSSIVNYGPALASVISKTNVLVVEGYLFEFPDTIKTI
Sbjct: 241 GTTGTVIVLTTPDAQRTMLAYQGTSSIVNYGPALASVISKTNVLVVEGYLFEFPDTIKTI 300

Query: 301 QKACEEAHRSGALVAVTGSDVSCIERHFDDFWEIVGNYADIVFTNHEEAGALCHFDSKES 360
           QKACEEAHRSGALVAVTGSDVSCIERHFDDFWEIVGNYADIVFTNHEEAGALCHFDSKES
Sbjct: 301 QKACEEAHRSGALVAVTGSDVSCIERHFDDFWEIVGNYADIVFTNHEEAGALCHFDSKES 360

Query: 361 SISAVRYLSHFVPLVSVTDGHRGSYLGVKGEAVYIPPYPCVPLDTCGAGDAYASGILYGI 420
           SISAVRYLSHFVPLVSVTDGHRGSYLGVKGEAVYIPPYPCVPLDTCGAGDAYASGILYGI
Sbjct: 361 SISAVRYLSHFVPLVSVTDGHRGSYLGVKGEAVYIPPYPCVPLDTCGAGDAYASGILYGI 420

Query: 421 MHGVTDLKQMGTFAAKIAARVVGQQGTRLSVQDAMELADSFSCFQSDIGSSHA 473
           MHGVTDLKQMGTFAAKIAARVVGQQGTRLSVQDAMELADSFSCFQSDIGSSHA
Sbjct: 421 MHGVTDLKQMGTFAAKIAARVVGQQGTRLSVQDAMELADSFSCFQSDIGSSHA 473

BLAST of Carg08420 vs. ExPASy TrEMBL
Match: A0A6J1K3F6 (uncharacterized protein LOC111490810 OS=Cucurbita maxima OX=3661 GN=LOC111490810 PE=3 SV=1)

HSP 1 Score: 914.4 bits (2362), Expect = 1.9e-262
Identity = 465/474 (98.10%), Postives = 467/474 (98.52%), Query Frame = 0

Query: 1   MSLYFPISSLPQFHLPFSSQPLSYPNLTPLHSLHFSSSSYLPSSSSTFIHRCTVGACFRF 60
           MSLYFPISSLPQFHLPFSSQPLSYPNLTPLHSLHFSSSSYLPSSSSTFIHRC V ACFRF
Sbjct: 1   MSLYFPISSLPQFHLPFSSQPLSYPNLTPLHSLHFSSSSYLPSSSSTFIHRCRVVACFRF 60

Query: 61  SSMAGAFDSFTGLSSCRVFSGDGTREFCSGSEEE--EEEEGEGNVSDEEFVVGIEESNDI 120
           SSMAG FDSFTGLSSCRVFSGDGTREFCSGSEEE  EEEE EGNVSDEEF VGIEESN+I
Sbjct: 61  SSMAGGFDSFTGLSSCRVFSGDGTREFCSGSEEEEQEEEEDEGNVSDEEFGVGIEESNEI 120

Query: 121 NGLPDRWDVLGLGQAMVDFSGMVDDEFLKKLGLEKGTRKLVNHEERGRVLRAMDGHSYKA 180
           NGLPDRWDVLGLGQAMVDFSGMVDDEFLKKLGLEKGTRKLVNHEERGRVLRAMDGHSYKA
Sbjct: 121 NGLPDRWDVLGLGQAMVDFSGMVDDEFLKKLGLEKGTRKLVNHEERGRVLRAMDGHSYKA 180

Query: 181 AAGGSLSNSLVALARLGIRPMKGPTFNVAMTGSVGSDPLGSFYRTKLRRANVHYLSPPVK 240
           AAGGSLSNSLVALARLGIRPMKGPTFNVAMTGSVGSDPLGSFYRTKLRRANVHYLSPPVK
Sbjct: 181 AAGGSLSNSLVALARLGIRPMKGPTFNVAMTGSVGSDPLGSFYRTKLRRANVHYLSPPVK 240

Query: 241 DGTTGTVIVLTTPDAQRTMLAYQGTSSIVNYGPALASVISKTNVLVVEGYLFEFPDTIKT 300
           DGTTGTVIVLTTPDAQRTMLAYQGTSSIVNYGPALASVISKTNVLVVEGYLFEFPDTIKT
Sbjct: 241 DGTTGTVIVLTTPDAQRTMLAYQGTSSIVNYGPALASVISKTNVLVVEGYLFEFPDTIKT 300

Query: 301 IQKACEEAHRSGALVAVTGSDVSCIERHFDDFWEIVGNYADIVFTNHEEAGALCHFDSKE 360
           IQKACEEAHRSGALVAVTGSDVSCIERHFDDFWEIVGNYADIVFTNHEEAGALCHFDSKE
Sbjct: 301 IQKACEEAHRSGALVAVTGSDVSCIERHFDDFWEIVGNYADIVFTNHEEAGALCHFDSKE 360

Query: 361 SSISAVRYLSHFVPLVSVTDGHRGSYLGVKGEAVYIPPYPCVPLDTCGAGDAYASGILYG 420
           SSISAVRYLSHFVPLVSVTDGHRGSYLGVKGEAVYIPPYPCVPLDTCGAGDAYASGILYG
Sbjct: 361 SSISAVRYLSHFVPLVSVTDGHRGSYLGVKGEAVYIPPYPCVPLDTCGAGDAYASGILYG 420

Query: 421 IMHGVTDLKQMGTFAAKIAARVVGQQGTRLSVQDAMELADSFSCFQSDIGSSHA 473
           IMHGV+DLKQMGTFAAKIAARVVGQQGTRLSVQDAMELADSFSCFQSDIGSSHA
Sbjct: 421 IMHGVSDLKQMGTFAAKIAARVVGQQGTRLSVQDAMELADSFSCFQSDIGSSHA 474

BLAST of Carg08420 vs. ExPASy TrEMBL
Match: A0A0A0LIH0 (PfkB domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G915160 PE=3 SV=1)

HSP 1 Score: 785.8 bits (2028), Expect = 1.0e-223
Identity = 414/477 (86.79%), Postives = 430/477 (90.15%), Query Frame = 0

Query: 1   MSLYFPISSLPQFHLPFSSQPLSYPNLTPLHSLHF-SSSSYLP-SSSSTFIHRCTVGACF 60
           MSLYFPISSLPQ  LPFSS PL  PN TP+ SL F SSSSY P SSSSTFIHRC      
Sbjct: 1   MSLYFPISSLPQIQLPFSSLPLPSPNFTPIPSLPFPSSSSYSPSSSSSTFIHRC------ 60

Query: 61  RFSSMAGAFDSFTGLSSCRVFSGDGTREFCSGSEEEEEEEGEGNVSDEEFVVGIEESNDI 120
           RFSSMAG FDSF    SCRVFSG GT EF SGSEEEEE++ EGN SDE+ +VGIE  +DI
Sbjct: 61  RFSSMAGGFDSFNAFFSCRVFSGYGTGEFSSGSEEEEEDD-EGNGSDEDLLVGIEHLHDI 120

Query: 121 NG----LPDRWDVLGLGQAMVDFSGMVDDEFLKKLGLEKGTRKLVNHEERGRVLRAMDGH 180
           N     LPDRWDVLGLGQAMVDFSGMVDDEFLKKLGLEKGTRK+VNHEERGRVLRAMDG 
Sbjct: 121 NPSSSCLPDRWDVLGLGQAMVDFSGMVDDEFLKKLGLEKGTRKVVNHEERGRVLRAMDGR 180

Query: 181 SYKAAAGGSLSNSLVALARLGIRPMKGPTFNVAMTGSVGSDPLGSFYRTKLRRANVHYLS 240
           SYKAAAGGSLSNSLVALARLGIRPMKGPTFNVAMTGS+GSDPLGSFYR+KLRRANVH+LS
Sbjct: 181 SYKAAAGGSLSNSLVALARLGIRPMKGPTFNVAMTGSIGSDPLGSFYRSKLRRANVHFLS 240

Query: 241 PPVKDGTTGTVIVLTTPDAQRTMLAYQGTSSIVNYGPALASVISKTNVLVVEGYLFEFPD 300
           PPVKDGTTGTVIVLTTPDAQRTMLAYQGTSS VNY PALASVISKTNVLVVEGYLFE PD
Sbjct: 241 PPVKDGTTGTVIVLTTPDAQRTMLAYQGTSSTVNYSPALASVISKTNVLVVEGYLFELPD 300

Query: 301 TIKTIQKACEEAHRSGALVAVTGSDVSCIERHFDDFWEIVGNYADIVFTNHEEAGALCHF 360
           TIKTIQKACEEAHRSGALVAVT SDVSCIERHFDDFWEIVGN+ADIVF NHEEA ALCHF
Sbjct: 301 TIKTIQKACEEAHRSGALVAVTASDVSCIERHFDDFWEIVGNFADIVFANHEEAAALCHF 360

Query: 361 DSKESSISAVRYLSHFVPLVSVTDGHRGSYLGVKGEAVYIPPYPCVPLDTCGAGDAYASG 420
           DSKESSISAVRYLSHFVPLVSVTDGHRGSY+GVKGEAVYIPP PCVPLDTCGAGDAYASG
Sbjct: 361 DSKESSISAVRYLSHFVPLVSVTDGHRGSYIGVKGEAVYIPPCPCVPLDTCGAGDAYASG 420

Query: 421 ILYGIMHGVTDLKQMGTFAAKIAARVVGQQGTRLSVQDAMELADSFSCFQSDIGSSH 472
           ILYGI+ GV+DLK+MGT AAKIAARVVGQQGTRL VQDAMELAD+FS FQSDIGSSH
Sbjct: 421 ILYGILQGVSDLKEMGTIAAKIAARVVGQQGTRLRVQDAMELADAFSSFQSDIGSSH 470

BLAST of Carg08420 vs. ExPASy TrEMBL
Match: A0A1S3CQ69 (uncharacterized sugar kinase slr0537 OS=Cucumis melo OX=3656 GN=LOC103503447 PE=3 SV=1)

HSP 1 Score: 780.4 bits (2014), Expect = 4.2e-222
Identity = 413/485 (85.15%), Postives = 429/485 (88.45%), Query Frame = 0

Query: 1   MSLYFPISSLPQFHLPFSSQPLSYPNLTPLHSL---------HFSSSSYLPSSSS-TFIH 60
           MSLYFPISSLPQ  LPFSS PLS PN TP+ SL           SSSSY PSS S TFIH
Sbjct: 1   MSLYFPISSLPQIQLPFSSPPLSSPNFTPIPSLPSPSSSSSSSSSSSSYSPSSPSFTFIH 60

Query: 61  RCTVGACFRFSSMAGAFDSFTGLSSCRVFSGDGTREFCSGSEEEEEEEGEGNVSDEEFVV 120
           RC      RFSSMAG FDSF    SCRVFSG GT EF SGSEEEEE++ EGN SDE+ +V
Sbjct: 61  RC------RFSSMAGGFDSFNAFVSCRVFSGYGTGEFSSGSEEEEEDD-EGNGSDEDLLV 120

Query: 121 GIEESNDIN----GLPDRWDVLGLGQAMVDFSGMVDDEFLKKLGLEKGTRKLVNHEERGR 180
           GIE  +DIN     LPDRWDVLGLGQAMVDFSGMVDDEFLKKLGLEKGTRK+VNHEERGR
Sbjct: 121 GIEHLHDINPSSSRLPDRWDVLGLGQAMVDFSGMVDDEFLKKLGLEKGTRKVVNHEERGR 180

Query: 181 VLRAMDGHSYKAAAGGSLSNSLVALARLGIRPMKGPTFNVAMTGSVGSDPLGSFYRTKLR 240
           VLRAMDG SYKAAAGGSLSNSLVALARLGIRPMKGPTFNVAMTGS+GSDPLGSFYR+KL 
Sbjct: 181 VLRAMDGRSYKAAAGGSLSNSLVALARLGIRPMKGPTFNVAMTGSIGSDPLGSFYRSKLS 240

Query: 241 RANVHYLSPPVKDGTTGTVIVLTTPDAQRTMLAYQGTSSIVNYGPALASVISKTNVLVVE 300
           RANVH+LSPPVKDGTTGTVIVLTTPDAQRTMLAYQGTSS VNY PALASVISKTNVLVVE
Sbjct: 241 RANVHFLSPPVKDGTTGTVIVLTTPDAQRTMLAYQGTSSTVNYSPALASVISKTNVLVVE 300

Query: 301 GYLFEFPDTIKTIQKACEEAHRSGALVAVTGSDVSCIERHFDDFWEIVGNYADIVFTNHE 360
           GYLFE PDTIKTIQKACEEAHRSGALVAVT SDVSCIERHFDDFWEIVGN+ADIVF NHE
Sbjct: 301 GYLFELPDTIKTIQKACEEAHRSGALVAVTASDVSCIERHFDDFWEIVGNFADIVFANHE 360

Query: 361 EAGALCHFDSKESSISAVRYLSHFVPLVSVTDGHRGSYLGVKGEAVYIPPYPCVPLDTCG 420
           EA ALCHFDSKESSISAVRYLSHFVPLVSVTDGHRGSY+GVKGEAVYIPP PCVPLDTCG
Sbjct: 361 EAAALCHFDSKESSISAVRYLSHFVPLVSVTDGHRGSYIGVKGEAVYIPPCPCVPLDTCG 420

Query: 421 AGDAYASGILYGIMHGVTDLKQMGTFAAKIAARVVGQQGTRLSVQDAMELADSFSCFQSD 472
           AGDAYASGILYGI+ GV+DLK+MGTFAAKIAARVVGQQGTRL VQDAMELAD+FS FQSD
Sbjct: 421 AGDAYASGILYGILQGVSDLKEMGTFAAKIAARVVGQQGTRLRVQDAMELADAFSSFQSD 478

BLAST of Carg08420 vs. ExPASy TrEMBL
Match: A0A6J1DX57 (uncharacterized protein LOC111025364 OS=Momordica charantia OX=3673 GN=LOC111025364 PE=3 SV=1)

HSP 1 Score: 753.4 bits (1944), Expect = 5.5e-214
Identity = 397/477 (83.23%), Postives = 419/477 (87.84%), Query Frame = 0

Query: 1   MSLYFPISSLPQFHLPFSSQPLSYPNLTPLHSLHFSSSSYLPSSSSTFIHRCTVGACFRF 60
           MSL FP+ SL          PLS P LT L SL   SS+Y P S+S+  HRC VG  F F
Sbjct: 1   MSLCFPLPSL----------PLSSPTLTLLRSLPLPSSAYHPPSASSSFHRCGVGDRFAF 60

Query: 61  SSMAGAFDSFTGLSSCRVFSGDGTREFCSGSEEEEEEEGEGNVSDEEFV--VGIEESNDI 120
            SMA   DSF GL SCRVF+GDGT+EF SGSEEE++++   N SDEEFV  +GI+   DI
Sbjct: 61  FSMARRRDSFRGLYSCRVFTGDGTQEFSSGSEEEDDDD---NASDEEFVPALGIQAPADI 120

Query: 121 NG----LPDRWDVLGLGQAMVDFSGMVDDEFLKKLGLEKGTRKLVNHEERGRVLRAMDGH 180
           NG    LPDRWDVLGLGQAMVDFSG+VDDEFLKKLGLEKGTRK+VNHEERGRVL+AMDG 
Sbjct: 121 NGSSSRLPDRWDVLGLGQAMVDFSGIVDDEFLKKLGLEKGTRKVVNHEERGRVLQAMDGR 180

Query: 181 SYKAAAGGSLSNSLVALARLGIRPMKGPTFNVAMTGSVGSDPLGSFYRTKLRRANVHYLS 240
           SYKAAAGGSLSNSLVALARLGIRPMK PTFNVAMTGSVGSDPLGSFYRTKLRRANVH+L 
Sbjct: 181 SYKAAAGGSLSNSLVALARLGIRPMKDPTFNVAMTGSVGSDPLGSFYRTKLRRANVHFLC 240

Query: 241 PPVKDGTTGTVIVLTTPDAQRTMLAYQGTSSIVNYGPALASVISKTNVLVVEGYLFEFPD 300
           PPVKDGTTGTVIVLTTPDAQRTMLAYQGTSS +NY PALASVISKTNVLVVEGYLFE PD
Sbjct: 241 PPVKDGTTGTVIVLTTPDAQRTMLAYQGTSSTLNYSPALASVISKTNVLVVEGYLFELPD 300

Query: 301 TIKTIQKACEEAHRSGALVAVTGSDVSCIERHFDDFWEIVGNYADIVFTNHEEAGALCHF 360
           TIKTIQKACEEAHRSGALVAVT SDVSCIERHFDDFWEIVGNYADIVF NHEEAGALCHF
Sbjct: 301 TIKTIQKACEEAHRSGALVAVTASDVSCIERHFDDFWEIVGNYADIVFANHEEAGALCHF 360

Query: 361 DSKESSISAVRYLSHFVPLVSVTDGHRGSYLGVKGEAVYIPPYPCVPLDTCGAGDAYASG 420
           DSKESSISAVRYLSHFVPLVSVTDGHRGSY+GVKGEAVYIPP PCVPLDTCGAGDAYASG
Sbjct: 361 DSKESSISAVRYLSHFVPLVSVTDGHRGSYIGVKGEAVYIPPSPCVPLDTCGAGDAYASG 420

Query: 421 ILYGIMHGVTDLKQMGTFAAKIAARVVGQQGTRLSVQDAMELADSFSCFQSDIGSSH 472
           ILYGI+HGV+DLK+MGTFAAKIAARVVGQQGTRL VQDAMELAD+FS FQSDIGSSH
Sbjct: 421 ILYGILHGVSDLKEMGTFAAKIAARVVGQQGTRLRVQDAMELADAFSSFQSDIGSSH 464

BLAST of Carg08420 vs. TAIR 10
Match: AT4G27600.1 (pfkB-like carbohydrate kinase family protein )

HSP 1 Score: 548.1 bits (1411), Expect = 6.8e-156
Identity = 301/474 (63.50%), Postives = 355/474 (74.89%), Query Frame = 0

Query: 2   SLYFPISSLPQFHLPFSSQPLSYPNLTPLHSLHFSSSSYLPSSSSTFIHRCTVGACFRFS 61
           SL+F +S++       +S   S  +L+ L+SL+    +    ++++F          R  
Sbjct: 14  SLFFTLSAIT------ASNNASSSSLSRLYSLYHPKGTLRTRTATSFF---GASGNLRIV 73

Query: 62  SMAGAFDSFTGLSSCRVFSGDGTREFCSGSEEEEEEEGEGNVSDEEFVVGIEESNDINGL 121
           SMA           CR+  G G  E     E E +  G  +  D+EF + +  +N     
Sbjct: 74  SMAE-----NRQLVCRIGGGAGA-ELDDMDEGEIQRIGNEDEDDDEF-IQVHANNSSAAS 133

Query: 122 PDRWDVLGLGQAMVDFSGMVDDEFLKKLGLEKGTRKLVNHEERGRVLRAMDGHSYKAAAG 181
           P+RWDVLGLGQAMVDFSG+VDDEFLKKLGLEKGTRKL+NHEERG++L+AMDG SYKAAAG
Sbjct: 134 PERWDVLGLGQAMVDFSGVVDDEFLKKLGLEKGTRKLINHEERGKLLQAMDGCSYKAAAG 193

Query: 182 GSLSNSLVALARLGIRPMKGPTFNVAMTGSVGSDPLGSFYRTKLRRANVHYLSPPVKDGT 241
           GSLSN+LVALARLG + +     NVAM GS+G DPLGSFY TKLRRANV++LS P+KDGT
Sbjct: 194 GSLSNTLVALARLGSQSICDRPLNVAMAGSIGGDPLGSFYGTKLRRANVNFLSAPIKDGT 253

Query: 242 TGTVIVLTTPDAQRTMLAYQGTSSIVNYGPALASVISKTNVLVVEGYLFEFPDTIKTIQK 301
           TGTVIVLTTPDAQRTMLAYQGTSS+VNY   LAS+I+KTNV VVEGYLFE PDTI+TI K
Sbjct: 254 TGTVIVLTTPDAQRTMLAYQGTSSVVNYDSCLASLIAKTNVFVVEGYLFELPDTIRTITK 313

Query: 302 ACEEAHRSGALVAVTGSDVSCIERHFDDFWEIVGNYADIVFTNHEEAGALCHFDSKESSI 361
           ACEEAHR+GALVAVT SDVSCIERH+DDFW+IVGNYADIVF N +EA A CHF ++ES I
Sbjct: 314 ACEEAHRNGALVAVTASDVSCIERHYDDFWDIVGNYADIVFANSDEARAFCHFSAEESPI 373

Query: 362 SAVRYLSHFVPLVSVTDGHRGSYLGVKGEAVYIPPYPCVPLDTCGAGDAYASGILYGIMH 421
           SA RY+SHFVP VSVTDG  GSY+GVKGEA+YIPP PCVP+DTCGAGDAYASGILYGI+ 
Sbjct: 374 SATRYMSHFVPFVSVTDGINGSYIGVKGEAIYIPPSPCVPVDTCGAGDAYASGILYGILR 433

Query: 422 GVTDLKQMGTFAAKIAARVVGQQGTRLSVQDAMELADSF------SCFQSDIGS 470
           GV+DLK MG  AA IAA VVGQQGTRL VQDA+ELA S       S  ++D+GS
Sbjct: 434 GVSDLKGMGDMAATIAATVVGQQGTRLRVQDAVELARSHAFRLNGSGVRTDVGS 471

BLAST of Carg08420 vs. TAIR 10
Match: AT1G19600.1 (pfkB-like carbohydrate kinase family protein )

HSP 1 Score: 73.9 bits (180), Expect = 3.8e-13
Identity = 79/331 (23.87%), Postives = 143/331 (43.20%), Query Frame = 0

Query: 127 VLGL-GQAMVDFSGMVDDEFLKKLGLEKGTRKLVNHEERGRVLRAMDGH----SYKAAAG 186
           VLGL   A++D    VD   L ++  ++G    V  +E   +L+ +D H      K  AG
Sbjct: 15  VLGLQPAALIDNVAPVDWSLLDQIPGDRGGSIAVQKDELEHMLKELDAHISVAPLKKMAG 74

Query: 187 GSLSNSLVALARLGIRPMKGPTFNVAMTGSVGSDPLGSFYRTKLRRANVHYLSPPVKDGT 246
           GS++N++  L+ +G     G      + G+ G D  G  + + +  + V       K G+
Sbjct: 75  GSVTNTVRGLS-VGFGVATG------IIGAYGDDEQGQLFVSNMGFSGVSISRLRKKKGS 134

Query: 247 TGTVIVLTTPDAQRTMLAYQGTSSIVNYGPALASVISKTNVLVVEGYLFEFPD-TIKTIQ 306
           T   + L      RTM     ++  +      A  +SK +    +  +  +    ++ IQ
Sbjct: 135 TAQCVCLVDDSGNRTMRPCLSSAVKIQ-----ADELSKEDFTGSKWLVLRYAVLNLQVIQ 194

Query: 307 KACEEAHRSGALVAVTGSDVSCIERHFDDFWEIV--GNYADIVFTNHEEAGALCHFDSKE 366
            A   A + G  V++  +    +     +  +++  GN  D+ F N +EA  L   + + 
Sbjct: 195 AAIRFAKQEGLSVSLDLASFEMVRNSKSELRQLLESGN-IDLCFANEDEAAELLRGEQEA 254

Query: 367 SSISAVRYLSHFVPLVSVTDGHRGSYLGVKGEAVYIPPY-PCVPLDTCGAGDAYASGILY 426
              +A+ +L        VT G +G       E V+I      V  D  GAGD +ASG LY
Sbjct: 255 GPEAALEFLGRHCRWAVVTLGSKGCIAKHDKEVVHISAIGETVATDATGAGDLFASGFLY 314

Query: 427 GIMHGVT--DLKQMGTFAAKIAARVVGQQGT 447
           G++ G++  +  ++G+ +     R +G + T
Sbjct: 315 GLIKGLSLEECCKVGSCSGGSVIRALGGEVT 332

BLAST of Carg08420 vs. TAIR 10
Match: AT3G09820.1 (adenosine kinase 1 )

HSP 1 Score: 62.0 bits (149), Expect = 1.5e-09
Identity = 77/350 (22.00%), Postives = 142/350 (40.57%), Query Frame = 0

Query: 115 SNDINGLPDRWDVLGLGQAMVDFSGMVDDEFLKKLGLEKGTRKLVNHEERGRVLRAMDGH 174
           S+D +G+     +LG+G  ++D S +VD +FL K  ++     L   +            
Sbjct: 3   SSDFDGI-----LLGMGNPLLDVSAVVDQQFLDKYDIKLNNAILAEDKHLPMYDEMSQKF 62

Query: 175 SYKAAAGGSLSNSLVALARLGIRPMKGPTFNVAMTGSVGSDPLGSFYRTKLRRANV---H 234
           + +  AGG+  NS + +A+  ++ + G T   +  GS+G D  G   +     A V   +
Sbjct: 63  NVEYIAGGATQNS-IKVAQWMLQ-VPGAT---SYMGSIGKDKYGEAMKKDATAAGVYVHY 122

Query: 235 YLSPPVKDGTTGTVIVLTTPDAQRTMLAYQGTSSIVNY----GPALASVISKTNVLVVEG 294
           Y       GT G  ++      +R+++A    ++         P   +++ K     + G
Sbjct: 123 YEDEATPTGTCGVCVL----GGERSLIANLSAANCYKVEHLKKPENWALVEKAKFYYIAG 182

Query: 295 -YLFEFPDTIKTIQKACEEAHRSGALVAVTGSDVSCIERHFDDFWEIVGNYADIVFTNHE 354
            +L   P++I+ +++     ++    V         I   F D  E    Y D +F N  
Sbjct: 183 FFLTVSPESIQLVREHAAANNK----VFTMNLSAPFICEFFKDVQEKCLPYMDYIFGNET 242

Query: 355 EAGALCHFDSKES-SISAVRYLSHFVPLVSVTDGH-----RGSYLGVKGEAVYIPPYPCV 414
           EA         E+  +  +      +P  S T        +G+   V  E   +  YP +
Sbjct: 243 EARTFSRVHGWETDDVEQIAIKMSQLPKASGTYKRTTVITQGADPVVVAEDGKVKKYPVI 302

Query: 415 PL------DTCGAGDAYASGILYGIMH--GVTDLKQMGTFAAKIAARVVG 443
           PL      DT GAGDA+  G L  ++H  G+ +  + G +A+ +  +  G
Sbjct: 303 PLPKEKLVDTNGAGDAFVGGFLSQLVHGKGIEECVRAGCYASNVVIQRSG 334

BLAST of Carg08420 vs. TAIR 10
Match: AT5G03300.1 (adenosine kinase 2 )

HSP 1 Score: 60.1 bits (144), Expect = 5.6e-09
Identity = 80/338 (23.67%), Postives = 133/338 (39.35%), Query Frame = 0

Query: 127 VLGLGQAMVDFSGMVDDEFLKKLGLEKGTRKLVNHEERGRVLRAMDGHSYKAAAGGSLSN 186
           +LG+G  ++D S +VDDEFL K  ++     L   +            + +  AGG+  N
Sbjct: 11  LLGMGNPLLDISAVVDDEFLTKYDIKLNNAILAEDKHLPMYDEMSSKFNVEYIAGGATQN 70

Query: 187 SL-VALARLGIRPMKGPTFNVAMTGSVGSDPLGSFYRTKLRRA--NVHYL-SPPVKDGTT 246
           S+ VA   L I    G T   +  GS+G D  G   +     A  NVHY        GT 
Sbjct: 71  SIKVAQWMLQI---PGAT---SYMGSIGKDKYGEAMKKDATAAGVNVHYYEDESAPTGTC 130

Query: 247 GTVIVLTTPDAQRTMLAYQGTSSIVNYG----PALASVISKTNVLVVEGYLFEFPDTIKT 306
           G  +V      +R+++A    ++         P   +++ K     + G+      + ++
Sbjct: 131 GVCVV----GGERSLIANLSAANCYKVDHLKKPENWALVEKAKFYYIAGFFLTV--SPES 190

Query: 307 IQKACEEAHRSGALVAVTGSDVSCIERHFDDFWEIVGNYADIVFTNHEEAGALCHFDSKE 366
           IQ   E A  +  +  +  S    I   F D  E    Y D VF N  EA         E
Sbjct: 191 IQLVSEHAAANNKVFTMNLS-APFICEFFKDVQEKFLPYMDFVFGNETEARTFSRVHGWE 250

Query: 367 S-SISAVRYLSHFVPLVSVTDGH-----RGSYLGVKGEAVYIPPYPCVPL------DTCG 426
           +  +  +      +P  + T        +G+   V  E   +  YP +PL      DT G
Sbjct: 251 TEDVEQIAIKISQLPKATGTYKRTTVITQGADPVVVAEDGKVKKYPVIPLPKEKLVDTNG 310

Query: 427 AGDAYASGILYGIM--HGVTDLKQMGTFAAKIAARVVG 443
           AGDA+  G +  ++    + +  + G +A+ +  +  G
Sbjct: 311 AGDAFVGGFMSQLVKEKSIEECVKAGCYASNVVIQRSG 335

BLAST of Carg08420 vs. TAIR 10
Match: AT5G58730.1 (pfkB-like carbohydrate kinase family protein )

HSP 1 Score: 49.3 bits (116), Expect = 9.9e-06
Identity = 32/118 (27.12%), Postives = 56/118 (47.46%), Query Frame = 0

Query: 356 SKESSISAVRYLSHFVPLVSVTDGHRGSYLGVKGEAVYIPPYPCVPLDTCGAGDAYASGI 415
           S E+    V  + H   +V VT+G +G  +  K + + +PP+    +D  GAGD++  G+
Sbjct: 198 SDEALFMDVEQMKHLCCVV-VTNGEKGCRIYHKDDEMTVPPFLAKQVDPTGAGDSFLGGL 257

Query: 416 LYGIMHGVT--DLKQMGTFAAKIAARVVGQQGTRLSVQDAMELADSFSCFQSDIGSSH 472
           + G++ G+T  D   +G     I    +GQ    L +   ++        Q +I SSH
Sbjct: 258 IVGLVEGLTVPDAALLGNLFGSITVEHIGQPKFDLMMLQKVKDEVQRRKKQCNISSSH 314

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7035625.12.1e-268100.00hypothetical protein SDJN02_02423 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022958117.11.7e-26599.37uncharacterized protein LOC111459440 [Cucurbita moschata][more]
XP_023534341.12.1e-26398.52uncharacterized protein LOC111795932 [Cucurbita pepo subsp. pepo][more]
XP_022995190.13.9e-26298.10uncharacterized protein LOC111490810 [Cucurbita maxima][more]
XP_038888543.12.6e-22687.00uncharacterized sugar kinase slr0537 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q554803.8e-3432.26Uncharacterized sugar kinase slr0537 OS=Synechocystis sp. (strain PCC 6803 / Kaz... [more]
A6M2292.6e-1124.825-dehydro-2-deoxygluconokinase OS=Clostridium beijerinckii (strain ATCC 51743 / ... [more]
Q0TUZ43.4e-1125.865-dehydro-2-deoxygluconokinase OS=Clostridium perfringens (strain ATCC 13124 / D... [more]
Q8XP783.4e-1125.865-dehydro-2-deoxygluconokinase OS=Clostridium perfringens (strain 13 / Type A) O... [more]
B2V4J81.3e-1026.865-dehydro-2-deoxygluconokinase OS=Clostridium botulinum (strain Alaska E43 / Typ... [more]
Match NameE-valueIdentityDescription
A0A6J1H1088.1e-26699.37uncharacterized protein LOC111459440 OS=Cucurbita moschata OX=3662 GN=LOC1114594... [more]
A0A6J1K3F61.9e-26298.10uncharacterized protein LOC111490810 OS=Cucurbita maxima OX=3661 GN=LOC111490810... [more]
A0A0A0LIH01.0e-22386.79PfkB domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G915160 PE=3 S... [more]
A0A1S3CQ694.2e-22285.15uncharacterized sugar kinase slr0537 OS=Cucumis melo OX=3656 GN=LOC103503447 PE=... [more]
A0A6J1DX575.5e-21483.23uncharacterized protein LOC111025364 OS=Momordica charantia OX=3673 GN=LOC111025... [more]
Match NameE-valueIdentityDescription
AT4G27600.16.8e-15663.50pfkB-like carbohydrate kinase family protein [more]
AT1G19600.13.8e-1323.87pfkB-like carbohydrate kinase family protein [more]
AT3G09820.11.5e-0922.00adenosine kinase 1 [more]
AT5G03300.15.6e-0923.67adenosine kinase 2 [more]
AT5G58730.19.9e-0627.12pfkB-like carbohydrate kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002139Ribokinase/fructokinasePRINTSPR00990RIBOKINASEcoord: 374..385
score: 35.19
coord: 333..348
score: 31.25
coord: 176..195
score: 42.78
IPR011611Carbohydrate kinase PfkBPFAMPF00294PfkBcoord: 171..451
e-value: 5.1E-37
score: 127.8
IPR029056Ribokinase-likeGENE3D3.40.1190.20coord: 122..456
e-value: 6.6E-70
score: 237.8
IPR029056Ribokinase-likeSUPERFAMILY53613Ribokinase-likecoord: 125..449
NoneNo IPR availablePANTHERPTHR43085:SF25KINASE, PUTATIVE, EXPRESSED-RELATEDcoord: 95..463
NoneNo IPR availablePANTHERPTHR43085HEXOKINASE FAMILY MEMBERcoord: 95..463
NoneNo IPR availableCDDcd01168adenosine_kinasecoord: 124..449
e-value: 1.57768E-99
score: 299.529
IPR002173Carbohydrate/purine kinase, PfkB, conserved sitePROSITEPS00584PFKB_KINASES_2coord: 403..416

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg08420-RACarg08420-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046835 carbohydrate phosphorylation
biological_process GO:0044281 small molecule metabolic process
biological_process GO:0019252 starch biosynthetic process
cellular_component GO:0009507 chloroplast
molecular_function GO:0019200 carbohydrate kinase activity
molecular_function GO:0016773 phosphotransferase activity, alcohol group as acceptor
molecular_function GO:0016301 kinase activity