Carg08415 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg08415
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	transmembrane ascorbate ferrireductase 1-like
Location	Carg_Chr02: 5867166 .. 5869114 (-)
RNA-Seq Expression	Carg08415
Synteny	Carg08415

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATGCCGGCGGTGAAAGCTTTTCCGTTCACCATAATCAGTCACGGGCTCGCAGTGGTGGCGCTCATCATGGTGCTCATTTGGACCATTCACTTCAGAGGCGGCCTTGCTTGGGAGTCTGTCAATAAGAGTCTCATCTTCAACGTAACTCTCTCCCTCTCTCTTTACCTTTCTTTTTTATTTTACTCATTTTTGAATTTTTCTTTTTAGGTTAAAAGTAATCTCAATTTTAAAAGTTTTAATTTGGATTTAATTTCAGCTTTTTTTCTTTAATTTTATAATTGTTACAAACCATTTGCATGTTTACCTTTATATATATGTATAATATACACATAGATCCATTCTTTTGTTTTTCAATAAAAGAAAAAAGAACGATATTTCCAACTTTTAAGTCCTACTTCTCACTTCCCACCTACTTTCATTAATGCTTTATTTGATGATTTGATTTTTAAATCATCTCACCATCTCATCATCTAAATCATAAATTTTTTATTTTTTTTCCGATTTAGATTGAATTTAAAACCTAAAAATTCACGTCAGTTTGTTGGTTGACTCGTTAGTTGACGTTTTTAAGGTTTAGTAAACCAGAAAATAGAGTTACAATCTCATCTTGCTTTTAAGATTATTATGAACATTAAATAAAATATTTAACTTGTGACTTATAAATGTTTGATATTTAAGTGACTTATAAATATTTGATATTTGAATTTTATAACTAAGGTTAGTTGTGACTTATAAATATTTGATATTTGAGTTTTATGAGATGTATTTTTTTAAATAATTGAAATACATTCGTAGACGAGTTATCATGGTTATCAATTCTATTCAAGTGGTTCAGGCCACCAACTTAGTCAATACATACTCTTAAATTTTATACTTTTATAATAAAAATCATCCATTAACCTTAAGAAAAAGTATTGTGGCCAAGACGATAACATGAATGAATACGAAAAAATTATGCAAGGTAAAAAATCCCACTCCATAAATAATGATTTATAGAATAGGTTGTATATTGAATAATTATATAAATTAGTGGTGTAAGGATTATCTAGATTTAGAACTAAGATAATCATACTATATAATAATTTTTTAAACAATATCATACTCTTAGAATCTTTTAGAATTAAGGTTTTAAAAACGACAATCTTAAATAATATATATATAGATTTAATTTTTTCGACTTAGTTAGTAAGTTTGAATTTTTTGGACGGGTCAACCGACCATCCTGAAATTACCCACGTTTACCTAAACAAATATTGCATATTAATTATCAGTTTTTTCCGCTCTCATTAGGTTCATCCTGTTTTTATGCTATTCGGATTGATCATCATAGGAGGCGAAGGTAATGATTTAATTAAATTCTATATTTTTAGTTAATTTCCATTTGAAAATAAAATTAATTATTAGTAATTCGAAATAATGCGTTCAAAAATCATGCAGCAATAATCAGTTATAAATCACTGCCTTTCAGAAAAGAAGTAAAGAAGGTGATCCATCTGGCGCTAAACGGCGTCGCATTGGTTCTAGGCATTGTGGGAATCAGCGCTGCATTCAAGAACCATAACGAGAGTGGGATCGCTAATCTTTACAGCTTACATTCCTGGATAGGAATTGGCGTAATTGTCCTCTACGGTATCCAGGTATTTACGTAATTGCCACCCATCATTTTAATTTCTTTAATTTTAATTTTTTAAACTCGTATTTATTTATAAGTTAAGATTTGAAGATTTTAAACTCGTATTTATTTATTTCGAAGAGCGACGCTGCCATGGCACGCGCTGTTGGGCGCGTGTGTGTACGTATTGGCCGCCGCCAACTCCGCGCTTGGCTTCCTTGAGAAGCTGACGTTTCTGCAGAGCTCCGGCGTCGCCAAATACGGCACTGAGGCGTTGCTCGTCAACTTCACCGCTATCACCACCGTCGTTTTTGCAGTGTTTGTGATTTTGTCT

mRNA sequence

ATGATGCCGGCGGTGAAAGCTTTTCCGTTCACCATAATCAGTCACGGGCTCGCAGTGGTGGCGCTCATCATGGTGCTCATTTGGACCATTCACTTCAGAGGCGGCCTTGCTTGGGAGTCTGTCAATAAGAGTCTCATCTTCAACGTTCATCCTGTTTTTATGCTATTCGGATTGATCATCATAGGAGGCGAAGCAATAATCAGTTATAAATCACTGCCTTTCAGAAAAGAAGTAAAGAAGGTGATCCATCTGGCGCTAAACGGCGTCGCATTGGTTCTAGGCATTGTGGGAATCAGCGCTGCATTCAAGAACCATAACGAGAGTGGGATCGCTAATCTTTACAGCTTACATTCCTGGATAGGAATTGGCGTAATTGTCCTCTACGGTATCCAGGTATTTACAGCGACGCTGCCATGGCACGCGCTGTTGGGCGCGTGTGTGTACGTATTGGCCGCCGCCAACTCCGCGCTTGGCTTCCTTGAGAAGCTGACGTTTCTGCAGAGCTCCGGCGTCGCCAAATACGGCACTGAGGCGTTGCTCGTCAACTTCACCGCTATCACCACCGTCGTTTTTGCAGTGTTTGTGATTTTGTCT

Coding sequence (CDS)

ATGATGCCGGCGGTGAAAGCTTTTCCGTTCACCATAATCAGTCACGGGCTCGCAGTGGTGGCGCTCATCATGGTGCTCATTTGGACCATTCACTTCAGAGGCGGCCTTGCTTGGGAGTCTGTCAATAAGAGTCTCATCTTCAACGTTCATCCTGTTTTTATGCTATTCGGATTGATCATCATAGGAGGCGAAGCAATAATCAGTTATAAATCACTGCCTTTCAGAAAAGAAGTAAAGAAGGTGATCCATCTGGCGCTAAACGGCGTCGCATTGGTTCTAGGCATTGTGGGAATCAGCGCTGCATTCAAGAACCATAACGAGAGTGGGATCGCTAATCTTTACAGCTTACATTCCTGGATAGGAATTGGCGTAATTGTCCTCTACGGTATCCAGGTATTTACAGCGACGCTGCCATGGCACGCGCTGTTGGGCGCGTGTGTGTACGTATTGGCCGCCGCCAACTCCGCGCTTGGCTTCCTTGAGAAGCTGACGTTTCTGCAGAGCTCCGGCGTCGCCAAATACGGCACTGAGGCGTTGCTCGTCAACTTCACCGCTATCACCACCGTCGTTTTTGCAGTGTTTGTGATTTTGTCT

Protein sequence

MMPAVKAFPFTIISHGLAVVALIMVLIWTIHFRGGLAWESVNKSLIFNVHPVFMLFGLIIIGGEAIISYKSLPFRKEVKKVIHLALNGVALVLGIVGISAAFKNHNESGIANLYSLHSWIGIGVIVLYGIQVFTATLPWHALLGACVYVLAAANSALGFLEKLTFLQSSGVAKYGTEALLVNFTAITTVVFAVFVILS

Homology

[Close]

BLAST of Carg08415 vs. NCBI nr
Match: KAG7035630.1 (Transmembrane ascorbate ferrireductase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 374.4 bits (960), Expect = 6.1e-100
Identity = 198/198 (100.00%), Postives = 198/198 (100.00%), Query Frame = 0


Query: 1   MMPAVKAFPFTIISHGLAVVALIMVLIWTIHFRGGLAWESVNKSLIFNVHPVFMLFGLII 60
           MMPAVKAFPFTIISHGLAVVALIMVLIWTIHFRGGLAWESVNKSLIFNVHPVFMLFGLII
Sbjct: 1   MMPAVKAFPFTIISHGLAVVALIMVLIWTIHFRGGLAWESVNKSLIFNVHPVFMLFGLII 60

Query: 61  IGGEAIISYKSLPFRKEVKKVIHLALNGVALVLGIVGISAAFKNHNESGIANLYSLHSWI 120
           IGGEAIISYKSLPFRKEVKKVIHLALNGVALVLGIVGISAAFKNHNESGIANLYSLHSWI
Sbjct: 61  IGGEAIISYKSLPFRKEVKKVIHLALNGVALVLGIVGISAAFKNHNESGIANLYSLHSWI 120

Query: 121 GIGVIVLYGIQVFTATLPWHALLGACVYVLAAANSALGFLEKLTFLQSSGVAKYGTEALL 180
           GIGVIVLYGIQVFTATLPWHALLGACVYVLAAANSALGFLEKLTFLQSSGVAKYGTEALL
Sbjct: 121 GIGVIVLYGIQVFTATLPWHALLGACVYVLAAANSALGFLEKLTFLQSSGVAKYGTEALL 180

Query: 181 VNFTAITTVVFAVFVILS 199
           VNFTAITTVVFAVFVILS
Sbjct: 181 VNFTAITTVVFAVFVILS 198

[Close]

BLAST of Carg08415 vs. NCBI nr
Match: XP_022957720.1 (transmembrane ascorbate ferrireductase 1-like [Cucurbita moschata])

HSP 1 Score: 356.7 bits (914), Expect = 1.3e-94
Identity = 194/214 (90.65%), Postives = 196/214 (91.59%), Query Frame = 0


Query: 1   MMPAVKAFPFTIISHGLAVVALIMVLIWTIHFRGGLAWESVNKSLIFNVHPVFMLFGLII 60
           MMPAVKAFPFT+ISHGLAVVALIMVLIWTIHFRGGLAWESVNKSLIFNVHPVFMLFGLII
Sbjct: 93  MMPAVKAFPFTVISHGLAVVALIMVLIWTIHFRGGLAWESVNKSLIFNVHPVFMLFGLII 152

Query: 61  IGGEAIISYKSLPFRKEVKKVIHLALNGVALVLGIVGISAAFKNHNESGIANLYSLHSWI 120
           IGGEAIISYKSLPFRKEVKKVIHLALNGVALVLGIVGISAAFKNHNESGIANLYSLHSWI
Sbjct: 153 IGGEAIISYKSLPFRKEVKKVIHLALNGVALVLGIVGISAAFKNHNESGIANLYSLHSWI 212

Query: 121 GIGVIVLYGIQ----------------VFTATLPWHALLGACVYVLAAANSALGFLEKLT 180
           GIGVIVLYGIQ                +  ATLPWHALLGACVYVLAAANSALGFLEKLT
Sbjct: 213 GIGVIVLYGIQWLYGFVTFFFPGGSADLRRATLPWHALLGACVYVLAAANSALGFLEKLT 272

Query: 181 FLQSSGVAKYGTEALLVNFTAITTVVFAVFVILS 199
           FLQSSGVAKYGTEALLVNFTAITTVVFAVFVILS
Sbjct: 273 FLQSSGVAKYGTEALLVNFTAITTVVFAVFVILS 306

[Close]

BLAST of Carg08415 vs. NCBI nr
Match: XP_023532942.1 (transmembrane ascorbate ferrireductase 1-like [Cucurbita pepo subsp. pepo] >XP_023532943.1 transmembrane ascorbate ferrireductase 1-like [Cucurbita pepo subsp. pepo] >XP_023532944.1 transmembrane ascorbate ferrireductase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 352.8 bits (904), Expect = 1.9e-93
Identity = 192/214 (89.72%), Postives = 194/214 (90.65%), Query Frame = 0


Query: 1   MMPAVKAFPFTIISHGLAVVALIMVLIWTIHFRGGLAWESVNKSLIFNVHPVFMLFGLII 60
           MMPAVKAFPFT+ISHGLAVV LIMVLIWTIHFRGGLAWESVNKSLIFNVHPVFMLFGLII
Sbjct: 1   MMPAVKAFPFTVISHGLAVVTLIMVLIWTIHFRGGLAWESVNKSLIFNVHPVFMLFGLII 60

Query: 61  IGGEAIISYKSLPFRKEVKKVIHLALNGVALVLGIVGISAAFKNHNESGIANLYSLHSWI 120
           IGGEAIISYKSLPFRKEVKKVIHLALNGVALVLGIVGI AAFKNHNESGIANLYSLHSWI
Sbjct: 61  IGGEAIISYKSLPFRKEVKKVIHLALNGVALVLGIVGIIAAFKNHNESGIANLYSLHSWI 120

Query: 121 GIGVIVLYGIQ----------------VFTATLPWHALLGACVYVLAAANSALGFLEKLT 180
           GIGVIVLYGIQ                +  ATLPWHALLGACVYVLAAANSALGFLEKLT
Sbjct: 121 GIGVIVLYGIQWLYGFVAFFYPGGSADLRRATLPWHALLGACVYVLAAANSALGFLEKLT 180

Query: 181 FLQSSGVAKYGTEALLVNFTAITTVVFAVFVILS 199
           FLQSSGVAKYGTEALLVNFTAITTVVFAVFVILS
Sbjct: 181 FLQSSGVAKYGTEALLVNFTAITTVVFAVFVILS 214

[Close]

BLAST of Carg08415 vs. NCBI nr
Match: BAE46406.1 (cytochrome b561 [Citrullus lanatus])

HSP 1 Score: 305.4 bits (781), Expect = 3.5e-79
Identity = 162/214 (75.70%), Postives = 179/214 (83.64%), Query Frame = 0


Query: 1   MMPAVKAFPFTIISHGLAVVALIMVLIWTIHFRGGLAWESVNKSLIFNVHPVFMLFGLII 60
           M  AVKAFPFT++SH LA+VALI+VL+WTIHFRGGLAWE+VNK+LIFN+HPV ML GLII
Sbjct: 1   MALAVKAFPFTVVSHVLAIVALILVLVWTIHFRGGLAWEAVNKNLIFNIHPVLMLLGLII 60

Query: 61  IGGEAIISYKSLPFRKEVKKVIHLALNGVALVLGIVGISAAFKNHNESGIANLYSLHSWI 120
           IGGEAIISYKSLP RKEVKKVIHL L+GVAL LGIVGI AAFKNHNESGIANLYSLHSWI
Sbjct: 61  IGGEAIISYKSLPLRKEVKKVIHLVLHGVALALGIVGIIAAFKNHNESGIANLYSLHSWI 120

Query: 121 GIGVIVLYGIQ----------------VFTATLPWHALLGACVYVLAAANSALGFLEKLT 180
           GIGVI LYG+Q                +  A+LPWH L G  +Y+LA  NSALGFLEKLT
Sbjct: 121 GIGVISLYGLQWVYGFVTFFYPGGSADLRRASLPWHVLFGITIYILAVGNSALGFLEKLT 180

Query: 181 FLQSSGVAKYGTEALLVNFTAITTVVFAVFVILS 199
           FL+SSG+AKYGTEA LVNFTAITTVVFA+FVILS
Sbjct: 181 FLESSGLAKYGTEAFLVNFTAITTVVFALFVILS 214

[Close]

BLAST of Carg08415 vs. NCBI nr
Match: XP_038888144.1 (transmembrane ascorbate ferrireductase 1 [Benincasa hispida])

HSP 1 Score: 305.4 bits (781), Expect = 3.5e-79
Identity = 162/214 (75.70%), Postives = 178/214 (83.18%), Query Frame = 0


Query: 1   MMPAVKAFPFTIISHGLAVVALIMVLIWTIHFRGGLAWESVNKSLIFNVHPVFMLFGLII 60
           M  AVKAFPFT++SH LA+VALIMVLIWTIHFRGGLAWE+VNK+LIFN+HPV ML GLII
Sbjct: 1   MALAVKAFPFTVVSHVLAIVALIMVLIWTIHFRGGLAWEAVNKNLIFNIHPVLMLLGLII 60

Query: 61  IGGEAIISYKSLPFRKEVKKVIHLALNGVALVLGIVGISAAFKNHNESGIANLYSLHSWI 120
           IGGEAIISYKSLP RKEVKKVIHL L+GVAL LGIVGI AAFKNHNESGIANLYSLHSWI
Sbjct: 61  IGGEAIISYKSLPLRKEVKKVIHLVLHGVALALGIVGIIAAFKNHNESGIANLYSLHSWI 120

Query: 121 GIGVIVLYGIQ----------------VFTATLPWHALLGACVYVLAAANSALGFLEKLT 180
           GIGVI LYG+Q                +   +LPWH + G  VY+LA  NSALGFLEKLT
Sbjct: 121 GIGVISLYGLQWVYGFVTFFYPGGSADLRRVSLPWHVIFGLTVYILAVGNSALGFLEKLT 180

Query: 181 FLQSSGVAKYGTEALLVNFTAITTVVFAVFVILS 199
           FL+SSG+AKYGTEA LVNFTAITTV+FA+FVILS
Sbjct: 181 FLESSGLAKYGTEAFLVNFTAITTVIFAIFVILS 214

[Close]

BLAST of Carg08415 vs. ExPASy Swiss-Prot
Match: Q8L856 (Transmembrane ascorbate ferrireductase 1 OS=Arabidopsis thaliana OX=3702 GN=CYB561A PE=1 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 1.3e-65
Identity = 132/214 (61.68%), Postives = 159/214 (74.30%), Query Frame = 0


Query: 1   MMPAVKAFPFTIISHGLAVVALIMVLIWTIHFRGGLAWESVNKSLIFNVHPVFMLFGLII 60
           M   + A   T ++H LAV+A IMVL+W+I +RGGLAWE+ NK+LIFN+HPV ML G II
Sbjct: 1   MAVRINAMAVTFVAHALAVIAAIMVLVWSISYRGGLAWEATNKNLIFNLHPVLMLIGFII 60

Query: 61  IGGEAIISYKSLPFRKEVKKVIHLALNGVALVLGIVGISAAFKNHNESGIANLYSLHSWI 120
           +GGEAIISYKSLP  K VKK+IHL L+ +AL LGI GI AAFKNHNES I NLYSLHSWI
Sbjct: 61  LGGEAIISYKSLPLEKPVKKLIHLILHAIALALGIFGICAAFKNHNESHIPNLYSLHSWI 120

Query: 121 GIGVIVLYGIQ------VF----------TATLPWHALLGACVYVLAAANSALGFLEKLT 180
           GIGVI LYG Q      VF          +  LPWHA+LG  VY+LA  N+ALGFLEKLT
Sbjct: 121 GIGVISLYGFQWVYSFIVFFFPGGSTNLKSGLLPWHAMLGLFVYILAVGNAALGFLEKLT 180

Query: 181 FLQSSGVAKYGTEALLVNFTAITTVVFAVFVILS 199
           FL++ G+ KYG+EA L+NFTAI T++F  FV+L+
Sbjct: 181 FLENGGLDKYGSEAFLINFTAIITILFGAFVVLT 214

[Close]

BLAST of Carg08415 vs. ExPASy Swiss-Prot
Match: C4IYS8 (Ascorbate-specific transmembrane electron transporter 2 OS=Zea mays OX=4577 PE=2 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 2.0e-61
Identity = 131/211 (62.09%), Postives = 151/211 (71.56%), Query Frame = 0


Query: 5   VKAFPFTIISHGLAVVALIMVLIWTIHFRGGLAWESVNKSLIFNVHPVFMLFGLIIIGGE 64
           V+A PFT  +H LAV A  MVL+W I+FRGGLA E+ NK+LIFNVHPV ML G IIIGGE
Sbjct: 7   VRAAPFTYAAHALAVAAAAMVLVWAIYFRGGLAIEATNKNLIFNVHPVLMLIGYIIIGGE 66

Query: 65  AIISYKSLP-FRKEVKKVIHLALNGVALVLGIVGISAAFKNHNESGIANLYSLHSWIGIG 124
           AI+ Y+ LP    E  K+IHL L+G+ALVLG VGI  AFKNHNESGIANLYSLHSWIGIG
Sbjct: 67  AIMVYRVLPTSNHETNKLIHLVLHGIALVLGAVGIYFAFKNHNESGIANLYSLHSWIGIG 126

Query: 125 VIVLYGIQ----------------VFTATLPWHALLGACVYVLAAANSALGFLEKLTFLQ 184
            I LYGIQ                V    LPWH L G  VY+LA AN+ LGFLEKLTFL+
Sbjct: 127 TITLYGIQWIVGFVTFFFPGAAPNVKKGVLPWHILFGLFVYILALANAELGFLEKLTFLE 186

Query: 185 SSGVAKYGTEALLVNFTAITTVVFAVFVILS 199
           SSG+ KYGTEA LVNFTA+  V+F   V+++
Sbjct: 187 SSGLDKYGTEAFLVNFTALVVVLFGASVVVA 217

[Close]

BLAST of Carg08415 vs. ExPASy Swiss-Prot
Match: Q6I681 (Ascorbate-specific transmembrane electron transporter 1 OS=Zea mays OX=4577 GN=ZCYB PE=1 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 4.4e-61
Identity = 130/211 (61.61%), Postives = 151/211 (71.56%), Query Frame = 0


Query: 5   VKAFPFTIISHGLAVVALIMVLIWTIHFRGGLAWESVNKSLIFNVHPVFMLFGLIIIGGE 64
           V+A PFT  +H LAV A  MVL+W+I FRGGLA ES NK+LIFNVHPV ML G +IIGGE
Sbjct: 7   VRAAPFTYAAHALAVAAAAMVLVWSIQFRGGLAIESTNKNLIFNVHPVLMLIGYVIIGGE 66

Query: 65  AIISYKSLP-FRKEVKKVIHLALNGVALVLGIVGISAAFKNHNESGIANLYSLHSWIGIG 124
           AI+ Y+ LP    +  K+IHL L+G+ALVLG VGI  AFKNHNESGIANLYSLHSWIGIG
Sbjct: 67  AIMVYRVLPTSNHDTTKLIHLILHGIALVLGAVGIYFAFKNHNESGIANLYSLHSWIGIG 126

Query: 125 VIVLYGIQ----------------VFTATLPWHALLGACVYVLAAANSALGFLEKLTFLQ 184
            I LYGIQ                V    LPWH L G  VY+LA AN+ LGFLEKLTFL+
Sbjct: 127 TITLYGIQWIIGFVTFFFPGAAPNVKKGVLPWHVLFGLFVYILALANAELGFLEKLTFLE 186

Query: 185 SSGVAKYGTEALLVNFTAITTVVFAVFVILS 199
           SSG+ KYGTEA LVNFTA+  V+F   V+++
Sbjct: 187 SSGLDKYGTEAFLVNFTALVVVLFGASVVVA 217

[Close]

BLAST of Carg08415 vs. ExPASy Swiss-Prot
Match: A3A9H6 (Probable ascorbate-specific transmembrane electron transporter 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0642300 PE=3 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 5.4e-59
Identity = 125/211 (59.24%), Postives = 149/211 (70.62%), Query Frame = 0


Query: 5   VKAFPFTIISHGLAVVALIMVLIWTIHFRGGLAWESVNKSLIFNVHPVFMLFGLIIIGGE 64
           V+A PFT ++H LAV A  MVL+W IHFRGGLA+E+ NK+LIFNVHPV ML G II+G E
Sbjct: 5   VRAAPFTYVAHALAVAAATMVLVWCIHFRGGLAFEATNKNLIFNVHPVLMLIGYIILGSE 64

Query: 65  AIISYKSLP-FRKEVKKVIHLALNGVALVLGIVGISAAFKNHNESGIANLYSLHSWIGIG 124
           AI+ YK LP ++ +  K+IHL L+ +ALV G VGI  AFK HNESGIANLYSLHSW+GIG
Sbjct: 65  AIMVYKVLPTWKHDTTKLIHLILHAIALVFGAVGIYCAFKFHNESGIANLYSLHSWLGIG 124

Query: 125 VIVLYGIQ----------------VFTATLPWHALLGACVYVLAAANSALGFLEKLTFLQ 184
            I LYGIQ                V    LPWH L G  VY+LA A + LGFLEKLTFLQ
Sbjct: 125 TICLYGIQWIFGFVAFFFPRASPSVRKGVLPWHILFGLFVYILALATAELGFLEKLTFLQ 184

Query: 185 SSGVAKYGTEALLVNFTAITTVVFAVFVILS 199
           SSG+ KYG EA LVNFTA+  V+F   V+++
Sbjct: 185 SSGLDKYGAEAFLVNFTALIVVLFGASVVVA 215

[Close]

BLAST of Carg08415 vs. ExPASy Swiss-Prot
Match: Q7XMK3 (Probable ascorbate-specific transmembrane electron transporter 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0533500 PE=2 SV=2)

HSP 1 Score: 218.8 bits (556), Expect = 5.6e-56
Identity = 121/211 (57.35%), Postives = 143/211 (67.77%), Query Frame = 0


Query: 5   VKAFPFTIISHGLAVVALIMVLIWTIHFRGGLAWESVNKSLIFNVHPVFMLFGLIIIGGE 64
           VKA PFT ++H LAV A +MVL+W I FRGGLA+E+ NK+LIFNVHPV ML G II+G E
Sbjct: 7   VKAAPFTYVAHALAVAAAVMVLVWCISFRGGLAFEADNKNLIFNVHPVLMLIGYIILGSE 66

Query: 65  AIISYKSLP-FRKEVKKVIHLALNGVALVLGIVGISAAFKNHNESGIANLYSLHSWIGIG 124
           AI+ YK  P    +  K+IHL L+ +A+VLG VGI  AFK HNESGIANLYSLHSW+GIG
Sbjct: 67  AIMIYKIFPKLNHDTTKLIHLILHAIAIVLGAVGIYCAFKFHNESGIANLYSLHSWLGIG 126

Query: 125 VIVLYGIQ----------------VFTATLPWHALLGACVYVLAAANSALGFLEKLTFLQ 184
            I LYGIQ                V    LPWH L G  VYVL  A + LG LEKLTFLQ
Sbjct: 127 TISLYGIQWIFGFVAFFYPGAAPHVRRGALPWHVLFGLFVYVLTLATAELGLLEKLTFLQ 186

Query: 185 SSGVAKYGTEALLVNFTAITTVVFAVFVILS 199
           SSG+ KYG EA LVNFT +   +F   V+++
Sbjct: 187 SSGLDKYGAEAFLVNFTGLVVALFGAAVVVA 217

[Close]

BLAST of Carg08415 vs. ExPASy TrEMBL
Match: A0A6J1H1B5 (transmembrane ascorbate ferrireductase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111459179 PE=4 SV=1)

HSP 1 Score: 356.7 bits (914), Expect = 6.3e-95
Identity = 194/214 (90.65%), Postives = 196/214 (91.59%), Query Frame = 0


Query: 1   MMPAVKAFPFTIISHGLAVVALIMVLIWTIHFRGGLAWESVNKSLIFNVHPVFMLFGLII 60
           MMPAVKAFPFT+ISHGLAVVALIMVLIWTIHFRGGLAWESVNKSLIFNVHPVFMLFGLII
Sbjct: 93  MMPAVKAFPFTVISHGLAVVALIMVLIWTIHFRGGLAWESVNKSLIFNVHPVFMLFGLII 152

Query: 61  IGGEAIISYKSLPFRKEVKKVIHLALNGVALVLGIVGISAAFKNHNESGIANLYSLHSWI 120
           IGGEAIISYKSLPFRKEVKKVIHLALNGVALVLGIVGISAAFKNHNESGIANLYSLHSWI
Sbjct: 153 IGGEAIISYKSLPFRKEVKKVIHLALNGVALVLGIVGISAAFKNHNESGIANLYSLHSWI 212

Query: 121 GIGVIVLYGIQ----------------VFTATLPWHALLGACVYVLAAANSALGFLEKLT 180
           GIGVIVLYGIQ                +  ATLPWHALLGACVYVLAAANSALGFLEKLT
Sbjct: 213 GIGVIVLYGIQWLYGFVTFFFPGGSADLRRATLPWHALLGACVYVLAAANSALGFLEKLT 272

Query: 181 FLQSSGVAKYGTEALLVNFTAITTVVFAVFVILS 199
           FLQSSGVAKYGTEALLVNFTAITTVVFAVFVILS
Sbjct: 273 FLQSSGVAKYGTEALLVNFTAITTVVFAVFVILS 306

[Close]

BLAST of Carg08415 vs. ExPASy TrEMBL
Match: Q3LGX5 (Cytochrome b561 OS=Citrullus lanatus OX=3654 GN=CLb561A PE=2 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 1.7e-79
Identity = 162/214 (75.70%), Postives = 179/214 (83.64%), Query Frame = 0


Query: 1   MMPAVKAFPFTIISHGLAVVALIMVLIWTIHFRGGLAWESVNKSLIFNVHPVFMLFGLII 60
           M  AVKAFPFT++SH LA+VALI+VL+WTIHFRGGLAWE+VNK+LIFN+HPV ML GLII
Sbjct: 1   MALAVKAFPFTVVSHVLAIVALILVLVWTIHFRGGLAWEAVNKNLIFNIHPVLMLLGLII 60

Query: 61  IGGEAIISYKSLPFRKEVKKVIHLALNGVALVLGIVGISAAFKNHNESGIANLYSLHSWI 120
           IGGEAIISYKSLP RKEVKKVIHL L+GVAL LGIVGI AAFKNHNESGIANLYSLHSWI
Sbjct: 61  IGGEAIISYKSLPLRKEVKKVIHLVLHGVALALGIVGIIAAFKNHNESGIANLYSLHSWI 120

Query: 121 GIGVIVLYGIQ----------------VFTATLPWHALLGACVYVLAAANSALGFLEKLT 180
           GIGVI LYG+Q                +  A+LPWH L G  +Y+LA  NSALGFLEKLT
Sbjct: 121 GIGVISLYGLQWVYGFVTFFYPGGSADLRRASLPWHVLFGITIYILAVGNSALGFLEKLT 180

Query: 181 FLQSSGVAKYGTEALLVNFTAITTVVFAVFVILS 199
           FL+SSG+AKYGTEA LVNFTAITTVVFA+FVILS
Sbjct: 181 FLESSGLAKYGTEAFLVNFTAITTVVFALFVILS 214

[Close]

BLAST of Carg08415 vs. ExPASy TrEMBL
Match: A0A6J1K9U2 (transmembrane ascorbate ferrireductase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111491396 PE=4 SV=1)

HSP 1 Score: 302.8 bits (774), Expect = 1.1e-78
Identity = 174/214 (81.31%), Postives = 179/214 (83.64%), Query Frame = 0


Query: 1   MMPAVKAFPFTIISHGLAVVALIMVLIWTIHFRGGLAWESVNKSLIFNVHPVFMLFGLII 60
           MMPAVKAFPFT+ISHGLAVVALIMVLIWT HF           +L  +VHPVFMLFGLII
Sbjct: 1   MMPAVKAFPFTVISHGLAVVALIMVLIWTFHF-----------NLSNSVHPVFMLFGLII 60

Query: 61  IGGEAIISYKSLPFRKEVKKVIHLALNGVALVLGIVGISAAFKNHNESGIANLYSLHSWI 120
           I GEAIISYKSLPF KEVK VIHLALNGVALVLGIVGISAAFK+HNESGIANLYSLHSWI
Sbjct: 61  IRGEAIISYKSLPFIKEVKMVIHLALNGVALVLGIVGISAAFKDHNESGIANLYSLHSWI 120

Query: 121 GIGVIVLYGIQ----------------VFTATLPWHALLGACVYVLAAANSALGFLEKLT 180
           GIGVIVLYGIQ                +  ATLPWHALLGACVYVLAAANSALGFLEKLT
Sbjct: 121 GIGVIVLYGIQWLYGFVSFFYPGGSADLRRATLPWHALLGACVYVLAAANSALGFLEKLT 180

Query: 181 FLQSSGVAKYGTEALLVNFTAITTVVFAVFVILS 199
           FLQSSGVAKYGTEALLVNFTAITTVVFAVFVILS
Sbjct: 181 FLQSSGVAKYGTEALLVNFTAITTVVFAVFVILS 203

[Close]

BLAST of Carg08415 vs. ExPASy TrEMBL
Match: A0A0A0LHJ1 (Cytochrome b561 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G914000 PE=4 SV=1)

HSP 1 Score: 294.7 bits (753), Expect = 3.0e-76
Identity = 155/214 (72.43%), Postives = 174/214 (81.31%), Query Frame = 0


Query: 1   MMPAVKAFPFTIISHGLAVVALIMVLIWTIHFRGGLAWESVNKSLIFNVHPVFMLFGLII 60
           M  AVKAFP T++SH + +VA+IMVL+WTIHFRGGLAWE+VNK+LIFN+HPV ML GLII
Sbjct: 1   MALAVKAFPLTVVSHVVGIVAMIMVLVWTIHFRGGLAWEAVNKNLIFNIHPVLMLLGLII 60

Query: 61  IGGEAIISYKSLPFRKEVKKVIHLALNGVALVLGIVGISAAFKNHNESGIANLYSLHSWI 120
           IGGEAI+SYKSLP RKEVKKVIHL L+GVAL LGIVGI AAFKNHNESGIANLYSLHSWI
Sbjct: 61  IGGEAIMSYKSLPLRKEVKKVIHLVLHGVALGLGIVGIIAAFKNHNESGIANLYSLHSWI 120

Query: 121 GIGVIVLYGIQ----------------VFTATLPWHALLGACVYVLAAANSALGFLEKLT 180
           GIGVI LYG+Q                +   +LPWH + G  VYVLA  NSALGFLEKLT
Sbjct: 121 GIGVISLYGLQWVYGFATFFYPGGSGDLRRVSLPWHVVFGLAVYVLAVGNSALGFLEKLT 180

Query: 181 FLQSSGVAKYGTEALLVNFTAITTVVFAVFVILS 199
           FL+S G+ KYGTEA LVNFTAITT++FAVFVILS
Sbjct: 181 FLESGGIPKYGTEAFLVNFTAITTIIFAVFVILS 214

[Close]

BLAST of Carg08415 vs. ExPASy TrEMBL
Match: A0A5A7TAA1 (Transmembrane ascorbate ferrireductase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G003680 PE=4 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 7.3e-75
Identity = 151/214 (70.56%), Postives = 172/214 (80.37%), Query Frame = 0


Query: 1   MMPAVKAFPFTIISHGLAVVALIMVLIWTIHFRGGLAWESVNKSLIFNVHPVFMLFGLII 60
           M  AVKAFP T++SH + +VA+I+VL+WTIHFRGGLAWE+VNK+LIFN+HP  ML GLI+
Sbjct: 1   MALAVKAFPLTVVSHVVGIVAVILVLVWTIHFRGGLAWEAVNKNLIFNIHPFLMLLGLIV 60

Query: 61  IGGEAIISYKSLPFRKEVKKVIHLALNGVALVLGIVGISAAFKNHNESGIANLYSLHSWI 120
           IGGEAIISYKSLP RKEVKKVIHL L+GVALVLGIVGI AAFKNHNESGIANLYSLHSWI
Sbjct: 61  IGGEAIISYKSLPLRKEVKKVIHLVLHGVALVLGIVGIIAAFKNHNESGIANLYSLHSWI 120

Query: 121 GIGVIVLYGIQ----------------VFTATLPWHALLGACVYVLAAANSALGFLEKLT 180
           GIGVI LY +Q                +   +LPWH + G  VY+LA  NSALGFLEKLT
Sbjct: 121 GIGVISLYALQWVYGFVTFFYPGGSADLRRVSLPWHVVFGLTVYILAVGNSALGFLEKLT 180

Query: 181 FLQSSGVAKYGTEALLVNFTAITTVVFAVFVILS 199
           FL+SSG+ KYGTEA LVNFTAITT++F VFVI S
Sbjct: 181 FLESSGIPKYGTEAFLVNFTAITTIIFGVFVIFS 214

[Close]

BLAST of Carg08415 vs. TAIR 10
Match: AT4G25570.1 (Cytochrome b561/ferric reductase transmembrane protein family )

HSP 1 Score: 250.8 bits (639), Expect = 9.4e-67
Identity = 132/214 (61.68%), Postives = 159/214 (74.30%), Query Frame = 0


Query: 1   MMPAVKAFPFTIISHGLAVVALIMVLIWTIHFRGGLAWESVNKSLIFNVHPVFMLFGLII 60
           M   + A   T ++H LAV+A IMVL+W+I +RGGLAWE+ NK+LIFN+HPV ML G II
Sbjct: 1   MAVRINAMAVTFVAHALAVIAAIMVLVWSISYRGGLAWEATNKNLIFNLHPVLMLIGFII 60

Query: 61  IGGEAIISYKSLPFRKEVKKVIHLALNGVALVLGIVGISAAFKNHNESGIANLYSLHSWI 120
           +GGEAIISYKSLP  K VKK+IHL L+ +AL LGI GI AAFKNHNES I NLYSLHSWI
Sbjct: 61  LGGEAIISYKSLPLEKPVKKLIHLILHAIALALGIFGICAAFKNHNESHIPNLYSLHSWI 120

Query: 121 GIGVIVLYGIQ------VF----------TATLPWHALLGACVYVLAAANSALGFLEKLT 180
           GIGVI LYG Q      VF          +  LPWHA+LG  VY+LA  N+ALGFLEKLT
Sbjct: 121 GIGVISLYGFQWVYSFIVFFFPGGSTNLKSGLLPWHAMLGLFVYILAVGNAALGFLEKLT 180

Query: 181 FLQSSGVAKYGTEALLVNFTAITTVVFAVFVILS 199
           FL++ G+ KYG+EA L+NFTAI T++F  FV+L+
Sbjct: 181 FLENGGLDKYGSEAFLINFTAIITILFGAFVVLT 214

[Close]

BLAST of Carg08415 vs. TAIR 10
Match: AT5G38630.1 (cytochrome B561-1 )

HSP 1 Score: 159.8 bits (403), Expect = 2.2e-39
Identity = 86/213 (40.38%), Postives = 124/213 (58.22%), Query Frame = 0


Query: 2   MPAVKAFPFTIISHGLAVVALIMVLIWTIHFRGGLAWESVNKSLIFNVHPVFMLFGLIII 61
           +P +  FP  ++   L  +   +VL WT+H+RGGLA  S NK  IFNVHPV M+ GLI+ 
Sbjct: 3   VPVLGGFPIFMVVRVLGFIIAALVLTWTVHYRGGLALSSDNKDHIFNVHPVMMVIGLILF 62

Query: 62  GGEAIISYKSLPFRKEVKKVIHLALNGVALVLGIVGISAAFKNHNESGIANLYSLHSWIG 121
            GEA+++YKS+   K +KK++HL L   A +L ++G+ AA K H + GI N YSLHSW+G
Sbjct: 63  NGEAMLAYKSVQGTKNLKKLVHLTLQLTAFILSLIGVWAALKFHIDKGIENFYSLHSWLG 122

Query: 122 IGVIVLYGIQ----------------VFTATLPWHALLGACVYVLAAANSALGFLEKLTF 181
           +  + L+  Q                   + +PWH  LG  +Y LA   +  G LEK+TF
Sbjct: 123 LACLFLFAFQWAAGFVTYWYPGGSRNSRASLMPWHVFLGISIYALALVTATTGILEKVTF 182

Query: 182 LQ-SSGVAKYGTEALLVNFTAITTVVFAVFVIL 198
           LQ +  + +Y TEA+LVN   +  ++   FVIL
Sbjct: 183 LQVNQVITRYSTEAMLVNTMGVLILILGGFVIL 215

[Close]

BLAST of Carg08415 vs. TAIR 10
Match: AT1G14730.1 (Cytochrome b561/ferric reductase transmembrane protein family )

HSP 1 Score: 116.3 bits (290), Expect = 2.8e-26
Identity = 66/204 (32.35%), Postives = 114/204 (55.88%), Query Frame = 0


Query: 11  TIISHGLAVVALIMVLIWTIHFRGGLAWESVNKSLIFNVHPVFMLFGLIIIGGEAIISYK 70
           T+++H   ++A++++LIW +H+R G+ + S N   + NVHP  M  G + + G+A+++YK
Sbjct: 18  TLVAHFFGILAVVLMLIWLLHYREGIEYGSDNPLKVLNVHPFLMYCGFLFLVGQAMMTYK 77

Query: 71  SLPFRKEVKKVIHLALNGVALVLGIVGISAAFKNHNESGIANLYSLHSWIGIGVIVLYGI 130
           +     +V+K++H  L+ + LVLGIVGI AAF+ H++  + ++ SLHSWIG+   +L G+
Sbjct: 78  TAYASHQVQKMVHGGLHLIGLVLGIVGICAAFRFHDKVNLKDMVSLHSWIGLTTFILLGV 137

Query: 131 Q----------------VFTATLPWHALLGACVYVLAAANSALGFLEKLTFLQSSGVAKY 190
           Q                  T  +PWH L G  +  +    +  G +++ T L  S  A  
Sbjct: 138 QWLFGAFTFLAPQSSSGTRTRMMPWHVLGGRALLYMGIVAALTGLMQRATMLGQSTNA-- 197

Query: 191 GTEALLVNFTAITTVVFAVFVILS 199
             E+ L+NF  +  ++F V V  S
Sbjct: 198 --ESRLINFLGLAILLFGVSVDFS 217

[Close]

BLAST of Carg08415 vs. TAIR 10
Match: AT1G26100.1 (Cytochrome b561/ferric reductase transmembrane protein family )

HSP 1 Score: 96.3 bits (238), Expect = 3.0e-20
Identity = 68/197 (34.52%), Postives = 101/197 (51.27%), Query Frame = 0


Query: 19  VVALIMVLIWTIHFRG-GLAWESVNKSLIFNVHPVFMLFGLIIIGGEAIISYKSLPFRKE 78
           +V  + VL W +     GL++ +        +HP+ M+ G I++ GEAI+ ++ LP  ++
Sbjct: 20  LVVAVSVLYWALFLPNLGLSYST--------LHPLLMVIGFILVSGEAILIHRWLPGSRK 79

Query: 79  VKKVIHLALNGVALVLGIVGISAAFKNHNESGI-ANLYSLHSWIGIGVIVLYGIQ----- 138
            KK +HL L G+AL   + GI   F  H + G+ AN YSLHSW+G+  + L+  Q     
Sbjct: 80  TKKAVHLWLQGMALASAVFGIWTKF--HYQRGVFANFYSLHSWMGLLSVSLFAAQWVTGF 139

Query: 139 -----------VFTATLPWHALLGACVYVLAAANSALGFLEKLTFLQSS-GVAKYGTEAL 197
                        T  LPWH  LG   Y LA A +  G LEKLTFLQ+   V + G+E++
Sbjct: 140 MSFWHRGEVRTTRTTFLPWHVFLGLYTYGLAIATAETGLLEKLTFLQTKRNVPRRGSESM 199

The following BLAST results are available for this feature:

BLAST of Carg08415 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7035630.1	6.1e-100	100.00	Transmembrane ascorbate ferrireductase 1, partial [Cucurbita argyrosperma subsp....	[more]
XP_022957720.1	1.3e-94	90.65	transmembrane ascorbate ferrireductase 1-like [Cucurbita moschata]	[more]
XP_023532942.1	1.9e-93	89.72	transmembrane ascorbate ferrireductase 1-like [Cucurbita pepo subsp. pepo] >XP_0...	[more]
BAE46406.1	3.5e-79	75.70	cytochrome b561 [Citrullus lanatus]	[more]
XP_038888144.1	3.5e-79	75.70	transmembrane ascorbate ferrireductase 1 [Benincasa hispida]	[more]

BLAST of Carg08415 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L856	1.3e-65	61.68	Transmembrane ascorbate ferrireductase 1 OS=Arabidopsis thaliana OX=3702 GN=CYB5...	[more]
C4IYS8	2.0e-61	62.09	Ascorbate-specific transmembrane electron transporter 2 OS=Zea mays OX=4577 PE=2...	[more]
Q6I681	4.4e-61	61.61	Ascorbate-specific transmembrane electron transporter 1 OS=Zea mays OX=4577 GN=Z...	[more]
A3A9H6	5.4e-59	59.24	Probable ascorbate-specific transmembrane electron transporter 1 OS=Oryza sativa...	[more]
Q7XMK3	5.6e-56	57.35	Probable ascorbate-specific transmembrane electron transporter 2 OS=Oryza sativa...	[more]

BLAST of Carg08415 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H1B5	6.3e-95	90.65	transmembrane ascorbate ferrireductase 1-like OS=Cucurbita moschata OX=3662 GN=L...	[more]
Q3LGX5	1.7e-79	75.70	Cytochrome b561 OS=Citrullus lanatus OX=3654 GN=CLb561A PE=2 SV=1	[more]
A0A6J1K9U2	1.1e-78	81.31	transmembrane ascorbate ferrireductase 1-like OS=Cucurbita maxima OX=3661 GN=LOC...	[more]
A0A0A0LHJ1	3.0e-76	72.43	Cytochrome b561 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G91...	[more]
A0A5A7TAA1	7.3e-75	70.56	Transmembrane ascorbate ferrireductase 1 OS=Cucumis melo var. makuwa OX=1194695 ...	[more]

BLAST of Carg08415 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G25570.1	9.4e-67	61.68	Cytochrome b561/ferric reductase transmembrane protein family	[more]
AT5G38630.1	2.2e-39	40.38	cytochrome B561-1	[more]
AT1G14730.1	2.8e-26	32.35	Cytochrome b561/ferric reductase transmembrane protein family	[more]
AT1G26100.1	3.0e-20	34.52	Cytochrome b561/ferric reductase transmembrane protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006593	Cytochrome b561/ferric reductase transmembrane	SMART	SM00665	561_7	coord: 49..159 e-value: 1.6E-34 score: 130.7
IPR006593	Cytochrome b561/ferric reductase transmembrane	PFAM	PF03188	Cytochrom_B561	coord: 49..134 e-value: 7.6E-27 score: 94.1
IPR006593	Cytochrome b561/ferric reductase transmembrane	PROSITE	PS50939	CYTOCHROME_B561	coord: 13..198 score: 24.76247
None	No IPR available	GENE3D	1.20.120.1770		coord: 1..198 e-value: 1.7E-63 score: 215.8
None	No IPR available	PANTHER	PTHR10106:SF22	TRANSMEMBRANE ASCORBATE FERRIREDUCTASE 1	coord: 3..198
IPR043205	Cytochrome b561/Cytochrome b reductase 1-like	PANTHER	PTHR10106	CYTOCHROME B561-RELATED	coord: 3..198

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg08415-RA	Carg08415-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016491	oxidoreductase activity