Carg08375 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg08375 vs. NCBI nr
Match: KAG7029809.1 (Afadin- and alpha-actinin-binding protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 716.5 bits (1848), Expect = 1.3e-202
Identity = 383/383 (100.00%), Postives = 383/383 (100.00%), Query Frame = 0

Query: 1   MAATKTDFDLSPSPQSIYSVGDYTFADVTNLDHCAKYLNQTLITFGFPASLDLFSNDPVS 60
           MAATKTDFDLSPSPQSIYSVGDYTFADVTNLDHCAKYLNQTLITFGFPASLDLFSNDPVS
Sbjct: 1   MAATKTDFDLSPSPQSIYSVGDYTFADVTNLDHCAKYLNQTLITFGFPASLDLFSNDPVS 60

Query: 61  IARTCNCIYFLLQQRQRDIDFRESANEQRQRLLLDISRLETKVERLESVIQAKDREIATI 120
           IARTCNCIYFLLQQRQRDIDFRESANEQRQRLLLDISRLETKVERLESVIQAKDREIATI
Sbjct: 61  IARTCNCIYFLLQQRQRDIDFRESANEQRQRLLLDISRLETKVERLESVIQAKDREIATI 120

Query: 121 TRTEAKAKAAFKAQIDKLQQEKEEFQRMVIGNQQVRTQQMHEMKKKEKEYIKLQERLNQV 180
           TRTEAKAKAAFKAQIDKLQQEKEEFQRMVIGNQQVRTQQMHEMKKKEKEYIKLQERLNQV
Sbjct: 121 TRTEAKAKAAFKAQIDKLQQEKEEFQRMVIGNQQVRTQQMHEMKKKEKEYIKLQERLNQV 180

Query: 181 LMEKKKESRSGMEIMNLLQKEGRQRGTWNGKKTDNDFYKKIVDTYEVKNQELMSENADLK 240
           LMEKKKESRSGMEIMNLLQKEGRQRGTWNGKKTDNDFYKKIVDTYEVKNQELMSENADLK
Sbjct: 181 LMEKKKESRSGMEIMNLLQKEGRQRGTWNGKKTDNDFYKKIVDTYEVKNQELMSENADLK 240

Query: 241 ALLRSMQVDMRDFLNAPNGLPKQPLAVNERVESEGARFSFGGRTDVFDLPFHMARDKIEE 300
           ALLRSMQVDMRDFLNAPNGLPKQPLAVNERVESEGARFSFGGRTDVFDLPFHMARDKIEE
Sbjct: 241 ALLRSMQVDMRDFLNAPNGLPKQPLAVNERVESEGARFSFGGRTDVFDLPFHMARDKIEE 300

Query: 301 SLRNKMASIKEQMGELQDAQKESEVTSEATERELELEAQLVEARSIILEQASIMSKHAKS 360
           SLRNKMASIKEQMGELQDAQKESEVTSEATERELELEAQLVEARSIILEQASIMSKHAKS
Sbjct: 301 SLRNKMASIKEQMGELQDAQKESEVTSEATERELELEAQLVEARSIILEQASIMSKHAKS 360

Query: 361 ERPRTQTIKLLDRMLRNGDEELR 384
           ERPRTQTIKLLDRMLRNGDEELR
Sbjct: 361 ERPRTQTIKLLDRMLRNGDEELR 383

BLAST of Carg08375 vs. NCBI nr
Match: KAG6598868.1 (Afadin- and alpha-actinin-binding protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 676.8 bits (1745), Expect = 1.1e-190
Identity = 361/365 (98.90%), Postives = 363/365 (99.45%), Query Frame = 0

Query: 1   MAATKTDFDLSPSPQSIYSVGDYTFADVTNLDHCAKYLNQTLITFGFPASLDLFSNDPVS 60
           MAATKTDFDLSPSPQSIYSVGDYTFADVTNLDHCAKYLNQTLITFGFPASLDLFSNDPVS
Sbjct: 1   MAATKTDFDLSPSPQSIYSVGDYTFADVTNLDHCAKYLNQTLITFGFPASLDLFSNDPVS 60

Query: 61  IARTCNCIYFLLQQRQRDIDFRESANEQRQRLLLDISRLETKVERLESVIQAKDREIATI 120
           IARTCNCIYFLLQQRQRDIDFRESANEQRQRLLLDISRLETKVERLESVIQAKDREIATI
Sbjct: 61  IARTCNCIYFLLQQRQRDIDFRESANEQRQRLLLDISRLETKVERLESVIQAKDREIATI 120

Query: 121 TRTEAKAKAAFKAQIDKLQQEKEEFQRMVIGNQQVRTQQMHEMKKKEKEYIKLQERLNQV 180
           TRTEAKAKAAFKAQIDKLQQEK+EFQRMVIGNQQVRTQQMHEMKKKEKEYIKLQERLNQV
Sbjct: 121 TRTEAKAKAAFKAQIDKLQQEKDEFQRMVIGNQQVRTQQMHEMKKKEKEYIKLQERLNQV 180

Query: 181 LMEKKKESRSGMEIMNLLQKEGRQRGTWNGKKTDNDFYKKIVDTYEVKNQELMSENADLK 240
           LMEKKKESRSGMEIMN LQKEGRQRGTWNGKKTDNDFYKKIVDTYEVKNQELMSENADLK
Sbjct: 181 LMEKKKESRSGMEIMNFLQKEGRQRGTWNGKKTDNDFYKKIVDTYEVKNQELMSENADLK 240

Query: 241 ALLRSMQVDMRDFLNAPNGLPKQPLAVNERVESEGARFSFGGRTDVFDLPFHMARDKIEE 300
           ALLRSMQVDMRDFLNAPNGLPKQPLAVNERVESEGARFSFGGRTDVFDLPFHMARDKIEE
Sbjct: 241 ALLRSMQVDMRDFLNAPNGLPKQPLAVNERVESEGARFSFGGRTDVFDLPFHMARDKIEE 300

Query: 301 SLRNKMASIKEQMGELQDAQKESEVTSEATERELELEAQLVEARSIILEQASIMSKHAKS 360
           SLRNKMASIKEQMGELQDAQKESEVTSEATERELELEAQLVEARSII EQASIMSKHAKS
Sbjct: 301 SLRNKMASIKEQMGELQDAQKESEVTSEATERELELEAQLVEARSIIQEQASIMSKHAKS 360

Query: 361 ERPRT 366
           ERPR+
Sbjct: 361 ERPRS 365

BLAST of Carg08375 vs. NCBI nr
Match: XP_023545625.1 (afadin- and alpha-actinin-binding protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 675.2 bits (1741), Expect = 3.2e-190
Identity = 360/365 (98.63%), Postives = 363/365 (99.45%), Query Frame = 0

Query: 1   MAATKTDFDLSPSPQSIYSVGDYTFADVTNLDHCAKYLNQTLITFGFPASLDLFSNDPVS 60
           MAATKTDFDLSPSPQSIYSVGDYTFADVTNLDHCAKYLNQTLITFGFPASLDLFSNDPVS
Sbjct: 24  MAATKTDFDLSPSPQSIYSVGDYTFADVTNLDHCAKYLNQTLITFGFPASLDLFSNDPVS 83

Query: 61  IARTCNCIYFLLQQRQRDIDFRESANEQRQRLLLDISRLETKVERLESVIQAKDREIATI 120
           IARTCNCIYFLLQQRQRDIDFRESANEQRQRLLLDISRLETKVERLESVIQAKDREIATI
Sbjct: 84  IARTCNCIYFLLQQRQRDIDFRESANEQRQRLLLDISRLETKVERLESVIQAKDREIATI 143

Query: 121 TRTEAKAKAAFKAQIDKLQQEKEEFQRMVIGNQQVRTQQMHEMKKKEKEYIKLQERLNQV 180
           TRTEAKAKAAFKAQIDKLQQEK+EFQRMVIGNQQVRTQQMHEMKKKEKEYIKLQERLNQV
Sbjct: 144 TRTEAKAKAAFKAQIDKLQQEKDEFQRMVIGNQQVRTQQMHEMKKKEKEYIKLQERLNQV 203

Query: 181 LMEKKKESRSGMEIMNLLQKEGRQRGTWNGKKTDNDFYKKIVDTYEVKNQELMSENADLK 240
           LMEKKKESRSGMEIMNLLQKEGRQRGTWNGKKTDNDFYKKIVDTYEVKNQELMSENADLK
Sbjct: 204 LMEKKKESRSGMEIMNLLQKEGRQRGTWNGKKTDNDFYKKIVDTYEVKNQELMSENADLK 263

Query: 241 ALLRSMQVDMRDFLNAPNGLPKQPLAVNERVESEGARFSFGGRTDVFDLPFHMARDKIEE 300
           ALLRSMQVDMRDFLNAPNGLPKQPLAVNERVESEGARFSFGGRTDVFDLPFHMARDKIEE
Sbjct: 264 ALLRSMQVDMRDFLNAPNGLPKQPLAVNERVESEGARFSFGGRTDVFDLPFHMARDKIEE 323

Query: 301 SLRNKMASIKEQMGELQDAQKESEVTSEATERELELEAQLVEARSIILEQASIMSKHAKS 360
           SLRNKMASIKE+MGELQDAQKESEVTSEATERE ELEAQLVEARSII EQASIMSKHAKS
Sbjct: 324 SLRNKMASIKERMGELQDAQKESEVTSEATEREFELEAQLVEARSIIQEQASIMSKHAKS 383

Query: 361 ERPRT 366
           ERPR+
Sbjct: 384 ERPRS 388

BLAST of Carg08375 vs. NCBI nr
Match: XP_022997425.1 (afadin- and alpha-actinin-binding protein-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 669.5 bits (1726), Expect = 1.8e-188
Identity = 357/365 (97.81%), Postives = 362/365 (99.18%), Query Frame = 0

Query: 1   MAATKTDFDLSPSPQSIYSVGDYTFADVTNLDHCAKYLNQTLITFGFPASLDLFSNDPVS 60
           MAATKTDFDLSPSPQSIYSVGDYTFADVTNLDHCAKYLNQTLITFGFPASLDLFSNDPVS
Sbjct: 1   MAATKTDFDLSPSPQSIYSVGDYTFADVTNLDHCAKYLNQTLITFGFPASLDLFSNDPVS 60

Query: 61  IARTCNCIYFLLQQRQRDIDFRESANEQRQRLLLDISRLETKVERLESVIQAKDREIATI 120
           IARTCNCIYFLLQQRQRDIDFRESANEQRQRLLLD+SRLETKVERLESVIQAKDREIATI
Sbjct: 61  IARTCNCIYFLLQQRQRDIDFRESANEQRQRLLLDLSRLETKVERLESVIQAKDREIATI 120

Query: 121 TRTEAKAKAAFKAQIDKLQQEKEEFQRMVIGNQQVRTQQMHEMKKKEKEYIKLQERLNQV 180
           TRTEAKAKAAFKAQIDKLQQEK+EFQRMVIGNQQVRTQQMHEMKKKEKEYIKLQERLNQV
Sbjct: 121 TRTEAKAKAAFKAQIDKLQQEKDEFQRMVIGNQQVRTQQMHEMKKKEKEYIKLQERLNQV 180

Query: 181 LMEKKKESRSGMEIMNLLQKEGRQRGTWNGKKTDNDFYKKIVDTYEVKNQELMSENADLK 240
           LMEKKKESRSGMEIMNLLQKEGRQRGTWNGKK DNDFYKKIVDTYEVKNQELMSENADLK
Sbjct: 181 LMEKKKESRSGMEIMNLLQKEGRQRGTWNGKKNDNDFYKKIVDTYEVKNQELMSENADLK 240

Query: 241 ALLRSMQVDMRDFLNAPNGLPKQPLAVNERVESEGARFSFGGRTDVFDLPFHMARDKIEE 300
           ALLRSMQVDMRDFLNAPNGLPKQPLAVNERVESEGA FSFGGRTDVFDLPFHMARDKIEE
Sbjct: 241 ALLRSMQVDMRDFLNAPNGLPKQPLAVNERVESEGAWFSFGGRTDVFDLPFHMARDKIEE 300

Query: 301 SLRNKMASIKEQMGELQDAQKESEVTSEATERELELEAQLVEARSIILEQASIMSKHAKS 360
           SLRNKMASIKE+MGELQDAQKESEVTSEA+ERELELEAQLVEARSII EQASIMSKHAKS
Sbjct: 301 SLRNKMASIKERMGELQDAQKESEVTSEASERELELEAQLVEARSIIQEQASIMSKHAKS 360

Query: 361 ERPRT 366
           ERPR+
Sbjct: 361 ERPRS 365

BLAST of Carg08375 vs. NCBI nr
Match: XP_038891085.1 (afadin- and alpha-actinin-binding protein isoform X2 [Benincasa hispida])

HSP 1 Score: 647.5 bits (1669), Expect = 7.2e-182
Identity = 350/369 (94.85%), Postives = 357/369 (96.75%), Query Frame = 0

Query: 1   MAATKTDFDLSPSPQSIYSVGDYTFADVTNLDHCAKYLNQTLITFGFPASLDLFSNDPVS 60
           MAAT+TDFDL PS QSIYSVGDYTFADVTNLDHCAKYLNQTLITFGFPASLDLFSNDPVS
Sbjct: 1   MAATETDFDLRPSSQSIYSVGDYTFADVTNLDHCAKYLNQTLITFGFPASLDLFSNDPVS 60

Query: 61  IARTCNCIYFLLQQRQRDIDFRESANEQRQRLLLDISRLETKVERLESVIQAKDREIATI 120
           IARTCNCIYFLLQQRQRDI+FRESANEQRQRLL DISRLETKVERLESVIQAKDREIATI
Sbjct: 61  IARTCNCIYFLLQQRQRDIEFRESANEQRQRLLSDISRLETKVERLESVIQAKDREIATI 120

Query: 121 TRTEAKAKAAFKAQIDKLQQEKEEFQRMVIGNQQVRTQQMHEMKKKEKEYIKLQERLNQV 180
           TRTEAKAKAAFKAQIDKLQQEK+EFQRMVIGNQQVRTQQ+HEMKKKEKEYIKLQERLNQV
Sbjct: 121 TRTEAKAKAAFKAQIDKLQQEKDEFQRMVIGNQQVRTQQIHEMKKKEKEYIKLQERLNQV 180

Query: 181 LMEKKKESRSGMEIMNLLQKEGRQRGTWNGKKTDNDFYKKIVDTYEVKNQELMSENADLK 240
           LMEKKKESRSGMEIMNLLQKEGRQRGTWNGKKTDNDFYKKIVD YEVKNQELMSENADLK
Sbjct: 181 LMEKKKESRSGMEIMNLLQKEGRQRGTWNGKKTDNDFYKKIVDAYEVKNQELMSENADLK 240

Query: 241 ALLRSMQVDMRDFLNAPNGLPKQPLAVNERVESEGARFSFGGRTDVFDLPFHMARDKIEE 300
           ALLRSMQVDMRDFLNAPNGL KQPLAVNERVESEGA+  FGGRTDVFDLPFHMARDKIEE
Sbjct: 241 ALLRSMQVDMRDFLNAPNGLHKQPLAVNERVESEGAQSPFGGRTDVFDLPFHMARDKIEE 300

Query: 301 SLRNKMASIKEQMGELQDAQKESEVTSEATERELELEAQLVEARSIILEQASIMSKH-AK 360
           SLRNKMASIKE+MG+LQDAQKESEVTSEATERELELEAQLVEARSII EQASIMSKH AK
Sbjct: 301 SLRNKMASIKERMGQLQDAQKESEVTSEATERELELEAQLVEARSIIQEQASIMSKHLAK 360

Query: 361 SERPRTQTI 369
           SERPR   I
Sbjct: 361 SERPRESII 369

BLAST of Carg08375 vs. ExPASy Swiss-Prot
Match: Q9Y2D8 (Afadin- and alpha-actinin-binding protein OS=Homo sapiens OX=9606 GN=SSX2IP PE=1 SV=3)

HSP 1 Score: 107.8 bits (268), Expect = 2.7e-22
Identity = 100/397 (25.19%), Postives = 196/397 (49.37%), Query Frame = 0

Query: 10  LSPSPQSIYSVGDYTFADVTNLDHCAKYLNQTLITFGFPA----SLDLFSNDPVSIARTC 69
           LS +  S +S     F    N++    YL+Q L TFGFP+    S    +   ++I    
Sbjct: 45  LSKNVHSFFS----AFCTEDNIEQSISYLDQELTTFGFPSLYEESKGKETKRELNIVAVL 104

Query: 70  NCIYFLLQQRQRDIDFRESANEQRQRLLLDISRLETKVERLESVIQAKDREIATITRTEA 129
           NC+  LL  +++++  +E+   Q  +L  D+  L++   +L+  ++   RE+  +   + 
Sbjct: 105 NCMNELLVLQRKNLLAQENVETQNLKLGSDMDHLQSCYSKLKEQLETSRREMIGLQERDR 164

Query: 130 KAKAAFKAQIDKLQQEKEEFQRMVIGNQQVRTQQMHEMKKKEKEYIKLQERLNQVLMEKK 189
           + +   +     L+ EK+E Q++        TQ  H+MK+KE+EY KL+ERL+Q++M  K
Sbjct: 165 QLQCKNRNLHQLLKNEKDEVQKLQNIIASRATQYNHDMKRKEREYNKLKERLHQLVM-NK 224

Query: 190 KESRSGMEIMNLLQKEGRQRGTWNGKKT----DNDFYKKIVDTYEVKNQELMSENADLKA 249
           K+ +  M+I+N + +   +RG+W   KT    +++ YK +++ YE + ++++ ENA+LK 
Sbjct: 225 KDKKIAMDILNYVGRADGKRGSWRTGKTEARNEDEMYKILLNDYEYRQKQILMENAELKK 284

Query: 250 LLRSMQVDMRDFLNAPNGLPKQPL-----AVNERVESEGARFSFGGRTDVFDLPFHMARD 309
           +L+ M+ +M   L+     P++ +      V   VE +    S   R  ++DL     R+
Sbjct: 285 VLQQMKKEMISLLSPQKKKPRERVDDSTGTVISDVEEDAGELS---RESMWDLSCETVRE 344

Query: 310 KIEESLRNKMASIKEQMGELQDA---------QKESEVTSEATERELE-LEAQLVEARSI 369
           ++  S+R +   +K  + +L +            E  ++ +  E+E E LE ++ + + +
Sbjct: 345 QLTNSIRKQWRILKSHVEKLDNQVSKVHLEGFNDEDVISRQDHEQETEKLELEIQQCKEM 404

Query: 370 ILEQASIMSKHAKSERPRTQTIKLLDRMLRNGDEELR 384
           I  Q  ++ +   +      T  L D  L    E L+
Sbjct: 405 IKTQQQLLQQQLATAYDDDTTSLLRDCYLLEEKERLK 433

BLAST of Carg08375 vs. ExPASy Swiss-Prot
Match: Q8CGZ2 (Afadin- and alpha-actinin-binding protein OS=Rattus norvegicus OX=10116 GN=Ssx2ip PE=1 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 7.8e-22
Identity = 100/397 (25.19%), Postives = 196/397 (49.37%), Query Frame = 0

Query: 11  SPSPQSIYSVGDYTFADVTNLDHCAKYLNQTLITFGFPA----SLDLFSNDPVSIARTCN 70
           +P  ++++ V    F    N++    YL+Q L TFGFP+    S    +   +SI    N
Sbjct: 43  TPLSKNVHGVFS-AFCTGENIEQSISYLDQELTTFGFPSLYEESKSKEAKRELSIVALLN 102

Query: 71  CIYFLLQQRQRDIDFRESANEQRQRLLLDISRLETKVERLESVIQAKDREIATITRTEAK 130
           C+  LL  +++++  +ES   Q  +L  D+  L++   +L+  ++A  RE+ ++   + +
Sbjct: 103 CMNELLVLQRKNLLAQESVETQNLKLGSDMDHLQSCYAKLKEQLEASRREMISLQERDRQ 162

Query: 131 AKAAFKAQIDKLQQEKEEFQRMVIGNQQVRTQQMHEMKKKEKEYIKLQERLNQVLMEKKK 190
            +   +     L+ EKEE Q++        TQ  H++K+KE+EY KL+ERL+Q++M  KK
Sbjct: 163 LQCKNRNLHQLLKNEKEEVQKLQNIIASRATQYNHDVKRKEREYNKLKERLHQLVM-NKK 222

Query: 191 ESRSGMEIMNLLQKEGRQRGTWNGKKT----DNDFYKKIVDTYEVKNQELMSENADLKAL 250
           +    M+++N + +   +RG+W   KT    +++ YK +++ YE + ++++ ENA+LK +
Sbjct: 223 DKNIAMDVLNYVGRVDGKRGSWRTDKTEARNEDEMYKILLNDYEYRQKQILLENAELKKV 282

Query: 251 LRSMQVDMRDFLNAPNGLPKQ------PLAVNERVESEGARFSFGGRTDVFDLPFHMARD 310
           L+ M+ +M   L+     P++         V   VE +    S   R  V+ L     R+
Sbjct: 283 LQQMKKEMISLLSPQKKKPRERAEDSTGTVVISDVEDDAGELS---RDGVWSLSCDTVRE 342

Query: 311 KIEESLRNKMASIKEQMGELQDA---------QKESEVTSEATERELE-LEAQLVEARSI 370
           ++  S+R +   +K  + +L +           +E  ++ +  E+E E LE ++   + +
Sbjct: 343 QLTNSIRKQWRILKSHVEKLDNQASKVHSEGFHEEDVISRQDHEQETEKLELEIERCKEM 402

Query: 371 ILEQASIMSKHAKSERPRTQTIKLLDRMLRNGDEELR 384
           I  Q  ++ +   +      T  L D  L    E L+
Sbjct: 403 IKAQQQLLQQQLATACDDDTTSLLRDCYLLEEKERLK 434

BLAST of Carg08375 vs. ExPASy Swiss-Prot
Match: Q8VC66 (Afadin- and alpha-actinin-binding protein OS=Mus musculus OX=10090 GN=Ssx2ip PE=1 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 5.1e-21
Identity = 97/399 (24.31%), Postives = 198/399 (49.62%), Query Frame = 0

Query: 10  LSPSPQSIYSVGDYTFADVTNLDHCAKYLNQTLITFGFPA----SLDLFSNDPVSIARTC 69
           LS +   ++ V    F    N++    YL+Q L TFGFP+    S    +   ++I    
Sbjct: 45  LSKNVHGVFGV----FCTGENIEQSISYLDQELTTFGFPSLYEESKSKEAKRELNIVAVL 104

Query: 70  NCIYFLLQQRQRDIDFRESANEQRQRLLLDISRLETKVERLESVIQAKDREIATITRTEA 129
           NC+  LL  +++++  +ES   Q  +L  D+  L++   +L+  ++   RE+  +   + 
Sbjct: 105 NCMNELLVLQRKNLLAQESVETQNLKLGSDMDHLQSCYAKLKEQLETSRREMIGLQERDR 164

Query: 130 KAKAAFKAQIDKLQQEKEEFQRMVIGNQQVRTQQMHEMKKKEKEYIKLQERLNQVLMEKK 189
           + +   ++    L+ EK+E Q++        TQ  H++K+KE+EY KL+ERL+Q++M  K
Sbjct: 165 QLQCKNRSLHQLLKNEKDEVQKLQNIIASRATQYNHDVKRKEREYNKLKERLHQLVM-NK 224

Query: 190 KESRSGMEIMNLLQKEGRQRGTWNGKKT----DNDFYKKIVDTYEVKNQELMSENADLKA 249
           K+    M+++N + +   +RG+W   KT    +++ YK +++ YE + ++++ ENA+LK 
Sbjct: 225 KDKNIAMDVLNYVGRADGKRGSWRTDKTEARNEDEMYKILLNDYEYRQKQILMENAELKK 284

Query: 250 LLRSMQVDMRDFLNAPNGLPKQ-------PLAVNERVESEGARFSFGGRTDVFDLPFHMA 309
           +L+ M+ +M   L+     P++        +A+++ +E +    S   R  V+ L     
Sbjct: 285 VLQQMKKEMISLLSPQKKKPRERAEDGTGTVAISD-IEDDSGELS---RDSVWGLSCDTV 344

Query: 310 RDKIEESLRNKMASIKEQMGEL---------QDAQKESEVTSEATERELE-LEAQLVEAR 369
           R+++  S+R +   +K  + +L         +   +E  ++ +  E+E E LE ++   +
Sbjct: 345 REQLTNSIRKQWRILKSHVEKLDNQASKVHSEGLNEEDVISRQDHEQETEKLELEIERCK 404

Query: 370 SIILEQASIMSKHAKSERPRTQTIKLLDRMLRNGDEELR 384
            +I  Q  ++ +   +      T  L D  L    E L+
Sbjct: 405 EMIKAQQQLLQQQLATTCDDDTTSLLRDCYLLEEKERLK 434

BLAST of Carg08375 vs. ExPASy Swiss-Prot
Match: Q6NRX3 (Afadin- and alpha-actinin-binding protein B OS=Xenopus laevis OX=8355 GN=ssx2ip-b PE=2 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 1.9e-20
Identity = 92/372 (24.73%), Postives = 181/372 (48.66%), Query Frame = 0

Query: 23  YTFADVTNLDHCAKYLNQTLITFGFPASLDLFSND---PVSIARTCNCIYFLLQQRQRDI 82
           Y F    N++ C  Y++Q L T GFP    +  N     + +    NCI+ LLQ+  +++
Sbjct: 46  YNFCTEDNIEQCITYIDQELRTIGFPTVQAVSKNGDGRKLHLVSIVNCIHELLQRNSQNM 105

Query: 83  DFRESANEQRQRLLLDISRLETKVERLESVIQAKDREIATITRTEAKAKAAFKAQIDKLQ 142
             +E    Q  ++  D+  L++  +R +  ++A  RE   +   + + +   +  +  L+
Sbjct: 106 RSKEEVETQLLKINGDLEHLQSIYQRQKDQMEATRRENCALQERDRQMQCKNRNLLQLLK 165

Query: 143 QEKEEFQRMVIGNQQVRTQQMHEMKKKEKEYIKLQERLNQVLMEKKKESRSGMEIMNLLQ 202
            EKEE Q++        TQ  H +K+KE+EY KL+ERL Q++M+ K++ +  ++++N + 
Sbjct: 166 NEKEEVQKLQNIIASRSTQFNHSVKRKEREYNKLKERLYQLVMD-KRDKKISIDVLNYVG 225

Query: 203 KEGRQRGTWNGKKTD----NDFYKKIVDTYEVKNQELMSENADLKALLRSMQVDMRDFLN 262
           +   +R +W   KTD     + YK +++ YE + ++LM ENA+LK +L+ M+ +M   L+
Sbjct: 226 RADGKRTSWRTGKTDAKNEEEMYKVLLNDYEQRQKQLMVENAELKKVLQQMKKEMISILS 285

Query: 263 APNGLPKQPLAVNERVESEGARFSFGGRTDVFDLPFHMARDKIEESLRNKMASIKEQMGE 322
                 K   ++           +   + ++ +L     R+++  S+R +   +K  M +
Sbjct: 286 QRKTKEKLDDSIGPVASDIEEDLADSSKENLSELSCEAVREQLVSSIRQQWRILKSHMEK 345

Query: 323 LQDAQKESE---VTSEATEREL-ELEAQLVEARSIILEQASIMSKHAKSERPRTQTIKLL 382
           L +     E   +     E+EL +L  ++ + +  I  Q  ++ +     R  T T+ L 
Sbjct: 346 LDNQATPDENGMIARGDHEQELGKLINEIQQCKETIKIQQQLLKQQFSVPRDDTSTL-LQ 405

Query: 383 DRMLRNGDEELR 384
           D  L    E L+
Sbjct: 406 DCYLLEDKERLQ 415

BLAST of Carg08375 vs. ExPASy Swiss-Prot
Match: Q6NRK1 (Afadin- and alpha-actinin-binding protein A OS=Xenopus laevis OX=8355 GN=ssx2ip-a PE=1 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 3.3e-20
Identity = 101/400 (25.25%), Postives = 189/400 (47.25%), Query Frame = 0

Query: 10  LSPSPQSIYSVGD------YTFADVTNLDHCAKYLNQTLITFGFPASLDLFSND---PVS 69
           L PSP   +SV +      YTF    NL+ C  Y++Q L T GFP    +  N     + 
Sbjct: 29  LLPSPS--HSVANNLSGSVYTFCTEDNLEQCITYIDQELRTIGFPTVQAVSKNGEGRKLH 88

Query: 70  IARTCNCIYFLLQQRQRDIDFRESANEQRQRLLLDISRLETKVERLESVIQAKDREIATI 129
           +    NCIY LLQ+  + +   E    Q  ++  D+  L++  +R +  ++A  RE   +
Sbjct: 89  LVSIINCIYELLQRNSQTMRSNEEVETQLLKINGDLEYLQSIHQRQKDQLEATKRENCAL 148

Query: 130 TRTEAKAKAAFKAQIDKLQQEKEEFQRMVIGNQQVRTQQMHEMKKKEKEYIKLQERLNQV 189
              + + +   +  +  L+ EKEE Q++        TQ  H +K+KE+EY KL+ERL Q+
Sbjct: 149 QERDRQMQCKNRNLLQLLKNEKEEVQKLQNIIASRSTQYNHSVKRKEREYNKLKERLYQL 208

Query: 190 LMEKKKESRSGMEIMNLLQKEGRQRGTWNGKKTD----NDFYKKIVDTYEVKNQELMSEN 249
           +M+ K++ +  ++++N + +   +R +W   KTD     + YK +++ YE + ++LM EN
Sbjct: 209 VMD-KRDKKISIDVLNYVGRADGKRSSWRTGKTDAKNEEEMYKVLLNDYEQRQKQLMVEN 268

Query: 250 ADLKALLRSMQVDMRDFLN---APNGLPKQPLAVNERVESEGARFSFGGRTDVFDLPFHM 309
            +LK +L+ M+ +M   ++       L      V   +E E A  S   + ++ +L    
Sbjct: 269 VELKKVLQQMKKEMISIVSQRKTKEKLEDSTGTVTSDIEEEIADSS---KENLSELSCEA 328

Query: 310 ARDKIEESLRNKMASIKEQMGELQD---------AQKESEVTSEATERELE-LEAQLVEA 369
            R+++  S+R +   +K  M +L +           +   +     E+EL+ L +++ + 
Sbjct: 329 VREQLISSIRQQWRILKSHMEKLDNQACLVNVPTPDENGLIARAEHEQELDKLISEIQQC 388

Query: 370 RSIILEQASIMSKHAKSERPRTQTIKLLDRMLRNGDEELR 384
           +  I  Q  ++ +     R    +  L D  L    E L+
Sbjct: 389 KETIRSQQQLLKQQLSVPRDDDTSKLLQDCYLLEDKERLQ 422

BLAST of Carg08375 vs. ExPASy TrEMBL
Match: A0A6J1K9L3 (afadin- and alpha-actinin-binding protein-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111492345 PE=3 SV=1)

HSP 1 Score: 669.5 bits (1726), Expect = 8.5e-189
Identity = 357/365 (97.81%), Postives = 362/365 (99.18%), Query Frame = 0

Query: 1   MAATKTDFDLSPSPQSIYSVGDYTFADVTNLDHCAKYLNQTLITFGFPASLDLFSNDPVS 60
           MAATKTDFDLSPSPQSIYSVGDYTFADVTNLDHCAKYLNQTLITFGFPASLDLFSNDPVS
Sbjct: 1   MAATKTDFDLSPSPQSIYSVGDYTFADVTNLDHCAKYLNQTLITFGFPASLDLFSNDPVS 60

Query: 61  IARTCNCIYFLLQQRQRDIDFRESANEQRQRLLLDISRLETKVERLESVIQAKDREIATI 120
           IARTCNCIYFLLQQRQRDIDFRESANEQRQRLLLD+SRLETKVERLESVIQAKDREIATI
Sbjct: 61  IARTCNCIYFLLQQRQRDIDFRESANEQRQRLLLDLSRLETKVERLESVIQAKDREIATI 120

Query: 121 TRTEAKAKAAFKAQIDKLQQEKEEFQRMVIGNQQVRTQQMHEMKKKEKEYIKLQERLNQV 180
           TRTEAKAKAAFKAQIDKLQQEK+EFQRMVIGNQQVRTQQMHEMKKKEKEYIKLQERLNQV
Sbjct: 121 TRTEAKAKAAFKAQIDKLQQEKDEFQRMVIGNQQVRTQQMHEMKKKEKEYIKLQERLNQV 180

Query: 181 LMEKKKESRSGMEIMNLLQKEGRQRGTWNGKKTDNDFYKKIVDTYEVKNQELMSENADLK 240
           LMEKKKESRSGMEIMNLLQKEGRQRGTWNGKK DNDFYKKIVDTYEVKNQELMSENADLK
Sbjct: 181 LMEKKKESRSGMEIMNLLQKEGRQRGTWNGKKNDNDFYKKIVDTYEVKNQELMSENADLK 240

Query: 241 ALLRSMQVDMRDFLNAPNGLPKQPLAVNERVESEGARFSFGGRTDVFDLPFHMARDKIEE 300
           ALLRSMQVDMRDFLNAPNGLPKQPLAVNERVESEGA FSFGGRTDVFDLPFHMARDKIEE
Sbjct: 241 ALLRSMQVDMRDFLNAPNGLPKQPLAVNERVESEGAWFSFGGRTDVFDLPFHMARDKIEE 300

Query: 301 SLRNKMASIKEQMGELQDAQKESEVTSEATERELELEAQLVEARSIILEQASIMSKHAKS 360
           SLRNKMASIKE+MGELQDAQKESEVTSEA+ERELELEAQLVEARSII EQASIMSKHAKS
Sbjct: 301 SLRNKMASIKERMGELQDAQKESEVTSEASERELELEAQLVEARSIIQEQASIMSKHAKS 360

Query: 361 ERPRT 366
           ERPR+
Sbjct: 361 ERPRS 365

BLAST of Carg08375 vs. ExPASy TrEMBL
Match: A0A5D3BQA4 (Afadin-and alpha-actinin-binding protein isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold29G00400 PE=3 SV=1)

HSP 1 Score: 644.4 bits (1661), Expect = 2.9e-181
Identity = 345/365 (94.52%), Postives = 354/365 (96.99%), Query Frame = 0

Query: 1   MAATKTDFDLSPSPQSIYSVGDYTFADVTNLDHCAKYLNQTLITFGFPASLDLFSNDPVS 60
           MAAT+TDFDL  SPQSIYSVGDYTFADV NLDHCAKYLNQTLITFGFPASLDLFSNDPVS
Sbjct: 1   MAATETDFDLRQSPQSIYSVGDYTFADVNNLDHCAKYLNQTLITFGFPASLDLFSNDPVS 60

Query: 61  IARTCNCIYFLLQQRQRDIDFRESANEQRQRLLLDISRLETKVERLESVIQAKDREIATI 120
           IARTCNCIYFLLQQRQRDI+FRESANEQRQRLL DISRLETKVERLESV+QAKDREIATI
Sbjct: 61  IARTCNCIYFLLQQRQRDIEFRESANEQRQRLLSDISRLETKVERLESVVQAKDREIATI 120

Query: 121 TRTEAKAKAAFKAQIDKLQQEKEEFQRMVIGNQQVRTQQMHEMKKKEKEYIKLQERLNQV 180
           TRTEAKAKAAFKAQIDKLQQEK+EFQRMVIGNQQVRTQQ+HEMKKKEKEYIKLQERLNQV
Sbjct: 121 TRTEAKAKAAFKAQIDKLQQEKDEFQRMVIGNQQVRTQQIHEMKKKEKEYIKLQERLNQV 180

Query: 181 LMEKKKESRSGMEIMNLLQKEGRQRGTWNGKKTDNDFYKKIVDTYEVKNQELMSENADLK 240
           LMEKKKESRSGMEIMNLLQKEGRQRGTWNGKKTDNDFYKKIVD YEVKNQELMSENADLK
Sbjct: 181 LMEKKKESRSGMEIMNLLQKEGRQRGTWNGKKTDNDFYKKIVDAYEVKNQELMSENADLK 240

Query: 241 ALLRSMQVDMRDFLNAPNGLPKQPLAVNERVESEGARFSFGGRTDVFDLPFHMARDKIEE 300
            LLRSMQVDMRDFLNAPNGLPKQPL +NERVESEGA+  FGGRTDVFDLPFHMARDKIEE
Sbjct: 241 TLLRSMQVDMRDFLNAPNGLPKQPLTINERVESEGAQSPFGGRTDVFDLPFHMARDKIEE 300

Query: 301 SLRNKMASIKEQMGELQDAQKESEVTSEATERELELEAQLVEARSIILEQASIMSKH-AK 360
           SLRNKMASIKE+MG+LQDAQKESEVTSEATERELELEAQLVEARSII EQASIMSKH AK
Sbjct: 301 SLRNKMASIKERMGQLQDAQKESEVTSEATERELELEAQLVEARSIIQEQASIMSKHLAK 360

Query: 361 SERPR 365
           SERPR
Sbjct: 361 SERPR 365

BLAST of Carg08375 vs. ExPASy TrEMBL
Match: A0A1S3BT77 (afadin- and alpha-actinin-binding protein isoform X4 OS=Cucumis melo OX=3656 GN=LOC103492899 PE=3 SV=1)

HSP 1 Score: 644.0 bits (1660), Expect = 3.8e-181
Identity = 345/369 (93.50%), Postives = 355/369 (96.21%), Query Frame = 0

Query: 1   MAATKTDFDLSPSPQSIYSVGDYTFADVTNLDHCAKYLNQTLITFGFPASLDLFSNDPVS 60
           MAAT+TDFDL  SPQSIYSVGDYTFADV NLDHCAKYLNQTLITFGFPASLDLFSNDPVS
Sbjct: 1   MAATETDFDLRQSPQSIYSVGDYTFADVNNLDHCAKYLNQTLITFGFPASLDLFSNDPVS 60

Query: 61  IARTCNCIYFLLQQRQRDIDFRESANEQRQRLLLDISRLETKVERLESVIQAKDREIATI 120
           IARTCNCIYFLLQQRQRDI+FRESANEQRQRLL DISRLETKVERLESV+QAKDREIATI
Sbjct: 61  IARTCNCIYFLLQQRQRDIEFRESANEQRQRLLSDISRLETKVERLESVVQAKDREIATI 120

Query: 121 TRTEAKAKAAFKAQIDKLQQEKEEFQRMVIGNQQVRTQQMHEMKKKEKEYIKLQERLNQV 180
           TRTEAK+KAAFKAQIDKLQQEK+EFQRMVIGNQQVRTQQ+HEMKKKEKEYIKLQERLNQV
Sbjct: 121 TRTEAKSKAAFKAQIDKLQQEKDEFQRMVIGNQQVRTQQIHEMKKKEKEYIKLQERLNQV 180

Query: 181 LMEKKKESRSGMEIMNLLQKEGRQRGTWNGKKTDNDFYKKIVDTYEVKNQELMSENADLK 240
           LMEKKKESRSGMEIMNLLQKEGRQRGTWNGKKTDNDFYKKIVD YEVKNQELMSENADLK
Sbjct: 181 LMEKKKESRSGMEIMNLLQKEGRQRGTWNGKKTDNDFYKKIVDAYEVKNQELMSENADLK 240

Query: 241 ALLRSMQVDMRDFLNAPNGLPKQPLAVNERVESEGARFSFGGRTDVFDLPFHMARDKIEE 300
            LLRSMQVDMRDFLNAPNGLPKQPL +NERVESEGA+  FGGRTDVFDLPFHMARDKIEE
Sbjct: 241 TLLRSMQVDMRDFLNAPNGLPKQPLTINERVESEGAQSPFGGRTDVFDLPFHMARDKIEE 300

Query: 301 SLRNKMASIKEQMGELQDAQKESEVTSEATERELELEAQLVEARSIILEQASIMSKH-AK 360
           SLRNKMASIKE+MG+LQDAQKESEVTSEATERELELEAQLVEARSII EQASIMSKH AK
Sbjct: 301 SLRNKMASIKERMGQLQDAQKESEVTSEATERELELEAQLVEARSIIQEQASIMSKHLAK 360

Query: 361 SERPRTQTI 369
           SERPR   I
Sbjct: 361 SERPRESII 369

BLAST of Carg08375 vs. ExPASy TrEMBL
Match: A0A1S3BT68 (afadin- and alpha-actinin-binding protein isoform X2 OS=Cucumis melo OX=3656 GN=LOC103492899 PE=3 SV=1)

HSP 1 Score: 643.3 bits (1658), Expect = 6.6e-181
Identity = 344/365 (94.25%), Postives = 354/365 (96.99%), Query Frame = 0

Query: 1   MAATKTDFDLSPSPQSIYSVGDYTFADVTNLDHCAKYLNQTLITFGFPASLDLFSNDPVS 60
           MAAT+TDFDL  SPQSIYSVGDYTFADV NLDHCAKYLNQTLITFGFPASLDLFSNDPVS
Sbjct: 1   MAATETDFDLRQSPQSIYSVGDYTFADVNNLDHCAKYLNQTLITFGFPASLDLFSNDPVS 60

Query: 61  IARTCNCIYFLLQQRQRDIDFRESANEQRQRLLLDISRLETKVERLESVIQAKDREIATI 120
           IARTCNCIYFLLQQRQRDI+FRESANEQRQRLL DISRLETKVERLESV+QAKDREIATI
Sbjct: 61  IARTCNCIYFLLQQRQRDIEFRESANEQRQRLLSDISRLETKVERLESVVQAKDREIATI 120

Query: 121 TRTEAKAKAAFKAQIDKLQQEKEEFQRMVIGNQQVRTQQMHEMKKKEKEYIKLQERLNQV 180
           TRTEAK+KAAFKAQIDKLQQEK+EFQRMVIGNQQVRTQQ+HEMKKKEKEYIKLQERLNQV
Sbjct: 121 TRTEAKSKAAFKAQIDKLQQEKDEFQRMVIGNQQVRTQQIHEMKKKEKEYIKLQERLNQV 180

Query: 181 LMEKKKESRSGMEIMNLLQKEGRQRGTWNGKKTDNDFYKKIVDTYEVKNQELMSENADLK 240
           LMEKKKESRSGMEIMNLLQKEGRQRGTWNGKKTDNDFYKKIVD YEVKNQELMSENADLK
Sbjct: 181 LMEKKKESRSGMEIMNLLQKEGRQRGTWNGKKTDNDFYKKIVDAYEVKNQELMSENADLK 240

Query: 241 ALLRSMQVDMRDFLNAPNGLPKQPLAVNERVESEGARFSFGGRTDVFDLPFHMARDKIEE 300
            LLRSMQVDMRDFLNAPNGLPKQPL +NERVESEGA+  FGGRTDVFDLPFHMARDKIEE
Sbjct: 241 TLLRSMQVDMRDFLNAPNGLPKQPLTINERVESEGAQSPFGGRTDVFDLPFHMARDKIEE 300

Query: 301 SLRNKMASIKEQMGELQDAQKESEVTSEATERELELEAQLVEARSIILEQASIMSKH-AK 360
           SLRNKMASIKE+MG+LQDAQKESEVTSEATERELELEAQLVEARSII EQASIMSKH AK
Sbjct: 301 SLRNKMASIKERMGQLQDAQKESEVTSEATERELELEAQLVEARSIIQEQASIMSKHLAK 360

Query: 361 SERPR 365
           SERPR
Sbjct: 361 SERPR 365

BLAST of Carg08375 vs. ExPASy TrEMBL
Match: A0A6J1DKI2 (afadin- and alpha-actinin-binding protein-like OS=Momordica charantia OX=3673 GN=LOC111020896 PE=3 SV=1)

HSP 1 Score: 641.0 bits (1652), Expect = 3.3e-180
Identity = 342/365 (93.70%), Postives = 356/365 (97.53%), Query Frame = 0

Query: 1   MAATKTDFDLSPSPQSIYSVGDYTFADVTNLDHCAKYLNQTLITFGFPASLDLFSNDPVS 60
           MAAT+TDFDL PSPQSIYSVGDYTFADVTNLDHCAKYLNQTLITFGFPASLDLFSNDPVS
Sbjct: 1   MAATETDFDLRPSPQSIYSVGDYTFADVTNLDHCAKYLNQTLITFGFPASLDLFSNDPVS 60

Query: 61  IARTCNCIYFLLQQRQRDIDFRESANEQRQRLLLDISRLETKVERLESVIQAKDREIATI 120
           IARTCNCIYFLLQQRQRDI++RESANEQRQRLL DISRLETKVERLESVIQAKDREIATI
Sbjct: 61  IARTCNCIYFLLQQRQRDIEYRESANEQRQRLLSDISRLETKVERLESVIQAKDREIATI 120

Query: 121 TRTEAKAKAAFKAQIDKLQQEKEEFQRMVIGNQQVRTQQMHEMKKKEKEYIKLQERLNQV 180
           TRT+ KAKAAFKAQI+KLQQEK+EFQRMVIGNQQVRTQQ+HEMKKKEK+YIKLQERLNQV
Sbjct: 121 TRTDTKAKAAFKAQIEKLQQEKDEFQRMVIGNQQVRTQQIHEMKKKEKDYIKLQERLNQV 180

Query: 181 LMEKKKESRSGMEIMNLLQKEGRQRGTWNGKKTDNDFYKKIVDTYEVKNQELMSENADLK 240
           LMEKKKESRSGMEIMNLLQKEGRQRGTWNGKKTDNDFYKKIVD YEVKNQELMSENADLK
Sbjct: 181 LMEKKKESRSGMEIMNLLQKEGRQRGTWNGKKTDNDFYKKIVDAYEVKNQELMSENADLK 240

Query: 241 ALLRSMQVDMRDFLNAPNGLPKQPLAVNERVESEGARFSFGGRTDVFDLPFHMARDKIEE 300
           ALLRSMQVDMRDF+NAPNGLPKQPLA+NERVESEGA+  FGGRTDVFDLPFHMARDKIEE
Sbjct: 241 ALLRSMQVDMRDFINAPNGLPKQPLALNERVESEGAQSPFGGRTDVFDLPFHMARDKIEE 300

Query: 301 SLRNKMASIKEQMGELQDAQKESEVTSEATERELELEAQLVEARSIILEQASIMSKH-AK 360
           SLRNKMASIKE+MG+LQDAQK +EVTSEATERELELEAQLVEARSII EQASIMSKH AK
Sbjct: 301 SLRNKMASIKERMGQLQDAQKGAEVTSEATERELELEAQLVEARSIIQEQASIMSKHLAK 360

Query: 361 SERPR 365
           SERPR
Sbjct: 361 SERPR 365

BLAST of Carg08375 vs. TAIR 10
Match: AT5G57410.1 (Afadin/alpha-actinin-binding protein )

HSP 1 Score: 501.9 bits (1291), Expect = 4.5e-142
Identity = 274/371 (73.85%), Postives = 310/371 (83.56%), Query Frame = 0

Query: 1   MAATKTDFDLSPSPQSIYSVGDYTFADVTNLDHCAKYLNQTLITFGFPASLDLFSNDPVS 60
           M A   ++DL    QS  S GDYTFA+  NL+HC KYLNQT++TFGFPASLDLFSNDPVS
Sbjct: 1   MPANDAEYDLKVLSQSA-SAGDYTFANEDNLEHCTKYLNQTMVTFGFPASLDLFSNDPVS 60

Query: 61  IARTCNCIYFLLQQRQRDIDFRESANEQRQRLLLDISRLETKVERLESVIQAKDREIATI 120
           I+RTCNC+Y LLQQRQRDI+FRESANE RQR   DI+RLE KVERLE+++Q KDREIATI
Sbjct: 61  ISRTCNCMYSLLQQRQRDIEFRESANELRQRQQSDIARLEAKVERLEALLQQKDREIATI 120

Query: 121 TRTEAKAKAAFKAQIDKLQQEKEEFQRMVIGNQQVRTQQMHEMKKKEKEYIKLQERLNQV 180
           TRTEAK  AA K+QI+KLQQE++EFQRMVIGNQQV+ QQ+HEMKKKEK+YIKLQERLNQV
Sbjct: 121 TRTEAKNTAALKSQIEKLQQERDEFQRMVIGNQQVKAQQIHEMKKKEKDYIKLQERLNQV 180

Query: 181 LMEKKKESRSGMEIMNLLQKEGRQRGTWNGKKTDNDFYKKIVDTYEVKNQELMSENADLK 240
           LMEKKKESRSGMEIMNLLQKEGRQRGTWNGKKTD DFYKKIVD YE KNQELM+EN  L+
Sbjct: 181 LMEKKKESRSGMEIMNLLQKEGRQRGTWNGKKTDTDFYKKIVDAYEAKNQELMAENTSLR 240

Query: 241 ALLRSMQVDMRDFLNAPNGLPKQPLAVNERVESEGARFSFGGRTDVFDLPFHMARDKIEE 300
           ALLRSMQ DMRDFLNAPNG     LA +E+ E++ ++   GG+TDVFDLP+ MAR +IEE
Sbjct: 241 ALLRSMQTDMRDFLNAPNG--SATLAGSEKREADPSQSPLGGKTDVFDLPYRMARGQIEE 300

Query: 301 SLRNKMASIKEQMGELQDAQKESEVTSEATERELELEAQLVEARSIILEQASIMSKHAKS 360
           SLR KMASIKE M +LQDA K + VTSEATERELELEAQLVEARSII EQ SIMSKH   
Sbjct: 301 SLRTKMASIKESMVQLQDAPKRASVTSEATERELELEAQLVEARSIIQEQESIMSKHLPK 360

Query: 361 ERPRTQTIKLL 372
              R +++  L
Sbjct: 361 SEQRRESVASL 368

BLAST of Carg08375 vs. TAIR 10
Match: AT5G57410.3 (Afadin/alpha-actinin-binding protein )

HSP 1 Score: 501.5 bits (1290), Expect = 5.9e-142
Identity = 279/378 (73.81%), Postives = 313/378 (82.80%), Query Frame = 0

Query: 1   MAATKTDFDLSPSPQSIYSVGDYTFADVTNLDHCAKYLNQTLITFGFPASLDLFSNDPVS 60
           M A   ++DL    QS  S GDYTFA+  NL+HC KYLNQT++TFGFPASLDLFSNDPVS
Sbjct: 1   MPANDAEYDLKVLSQSA-SAGDYTFANEDNLEHCTKYLNQTMVTFGFPASLDLFSNDPVS 60

Query: 61  IARTCNCIYFLLQQRQRDIDFRESANEQRQRLLLDISRLETKVERLESVIQAKDREIATI 120
           I+RTCNC+Y LLQQRQRDI+FRESANE RQR   DI+RLE KVERLE+++Q KDREIATI
Sbjct: 61  ISRTCNCMYSLLQQRQRDIEFRESANELRQRQQSDIARLEAKVERLEALLQQKDREIATI 120

Query: 121 TRTEAKAKAAFKAQIDKLQQEKEEFQRMVIGNQQVRTQQMHEMKKKEKEYIKLQERLNQV 180
           TRTEAK  AA K+QI+KLQQE++EFQRMVIGNQQV+ QQ+HEMKKKEK+YIKLQERLNQV
Sbjct: 121 TRTEAKNTAALKSQIEKLQQERDEFQRMVIGNQQVKAQQIHEMKKKEKDYIKLQERLNQV 180

Query: 181 LMEKKKESRSGMEIMNLLQKEGRQRGTWNGKKTDNDFYKKIVDTYEVKNQELMSENADLK 240
           LMEKKKESRSGMEIMNLLQKEGRQRGTWNGKKTD DFYKKIVD YE KNQELM+EN  L+
Sbjct: 181 LMEKKKESRSGMEIMNLLQKEGRQRGTWNGKKTDTDFYKKIVDAYEAKNQELMAENTSLR 240

Query: 241 ALLRSMQVDMRDFLNAPNGLPKQPLAVNERVESEGARFSFGGRTDVFDLPFHMARDKIEE 300
           ALLRSMQ DMRDFLNAPNG     LA +E+ E++ ++   GG+TDVFDLP+ MAR +IEE
Sbjct: 241 ALLRSMQTDMRDFLNAPNG--SATLAGSEKREADPSQSPLGGKTDVFDLPYRMARGQIEE 300

Query: 301 SLRNKMASIKEQMGELQDAQKESEVTSEATERELELEAQLVEARSIILEQASIMSKH-AK 360
           SLR KMASIKE M +LQDA K + VTSEATERELELEAQLVEARSII EQ SIMSKH  K
Sbjct: 301 SLRTKMASIKESMVQLQDAPKRASVTSEATERELELEAQLVEARSIIQEQESIMSKHLPK 360

Query: 361 SERPRTQTIKLLDRMLRN 378
           SE+ R     L    L N
Sbjct: 361 SEQRRESVASLAAEGLDN 375

BLAST of Carg08375 vs. TAIR 10
Match: AT2G18876.1 (Afadin/alpha-actinin-binding protein )

HSP 1 Score: 475.3 bits (1222), Expect = 4.5e-134
Identity = 258/368 (70.11%), Postives = 307/368 (83.42%), Query Frame = 0

Query: 1   MAATKTDFDLSPSPQSIYS---VGDYTFADVTNLDHCAKYLNQTLITFGFPASLDLFSND 60
           M+ T  +FD   +PQ  +S   +G+  FADV NL++C KYLNQ+L+T GF ASLDLF+ D
Sbjct: 1   MSLTVPEFDFRLTPQFGFSQSPIGERNFADVDNLENCIKYLNQSLVTSGFSASLDLFATD 60

Query: 61  PVSIARTCNCIYFLLQQRQRDIDFRESANEQRQRLLLDISRLETKVERLESVIQAKDREI 120
           PVSIARTCNC+Y L+QQRQRD++FRESAN+QRQRLL D++RLE KVERLE+ +QAK+RE+
Sbjct: 61  PVSIARTCNCVYALIQQRQRDVEFRESANDQRQRLLSDMARLEAKVERLETQLQAKEREL 120

Query: 121 ATITRTEAKAKAAFKAQIDKLQQEKEEFQRMVIGNQQVRTQQMHEMKKKEKEYIKLQERL 180
            ++TRTEAK  AA K Q +KLQ+E++EFQRMVI NQQV+TQQ+HE KKKEKEYIKLQERL
Sbjct: 121 GSVTRTEAKNTAALKTQNEKLQKERDEFQRMVIANQQVKTQQLHETKKKEKEYIKLQERL 180

Query: 181 NQVLMEKKKESRSGMEIMNLLQKEGRQRGTWNGKKTDNDFYKKIVDTYEVKNQELMSENA 240
           NQVLMEKKKE+RSGMEIMNLLQKEGRQRGTW+GKKTD+DFYKKIVD YE KNQELM+EN 
Sbjct: 181 NQVLMEKKKETRSGMEIMNLLQKEGRQRGTWSGKKTDSDFYKKIVDAYEAKNQELMAENT 240

Query: 241 DLKALLRSMQVDMRDFLNAPNGLPKQPLAVNERVESEGARFSFGGRTDVFDLPFHMARDK 300
           DL+ALLRS Q DMR FLNA  GL  Q L  N R  ++ ++   GG+TDVFDLPF MAR +
Sbjct: 241 DLRALLRSTQGDMRSFLNASGGLTNQSLVANGRHGADPSQSPLGGKTDVFDLPFRMARGQ 300

Query: 301 IEESLRNKMASIKEQMGELQDAQKESEVTSEATERELELEAQLVEARSIILEQASIMSKH 360
           IE+SLR+KM SIKE+MG+L DAQKE  +TSEA+ERELELEAQLVEARSII EQ SIMSKH
Sbjct: 301 IEDSLRSKMVSIKERMGQLVDAQKEVSITSEASERELELEAQLVEARSIIQEQESIMSKH 360

Query: 361 -AKSERPR 365
             K++R R
Sbjct: 361 LPKTDRRR 368

BLAST of Carg08375 vs. TAIR 10
Match: AT5G57410.2 (Afadin/alpha-actinin-binding protein )

HSP 1 Score: 459.1 bits (1180), Expect = 3.4e-129
Identity = 251/330 (76.06%), Postives = 283/330 (85.76%), Query Frame = 0

Query: 42  LITFGFPASLDLFSNDPVSIARTCNCIYFLLQQRQRDIDFRESANEQRQRLLLDISRLET 101
           ++TFGFPASLDLFSNDPVSI+RTCNC+Y LLQQRQRDI+FRESANE RQR   DI+RLE 
Sbjct: 1   MVTFGFPASLDLFSNDPVSISRTCNCMYSLLQQRQRDIEFRESANELRQRQQSDIARLEA 60

Query: 102 KVERLESVIQAKDREIATITRTEAKAKAAFKAQIDKLQQEKEEFQRMVIGNQQVRTQQMH 161
           KVERLE+++Q KDREIATITRTEAK  AA K+QI+KLQQE++EFQRMVIGNQQV+ QQ+H
Sbjct: 61  KVERLEALLQQKDREIATITRTEAKNTAALKSQIEKLQQERDEFQRMVIGNQQVKAQQIH 120

Query: 162 EMKKKEKEYIKLQERLNQVLMEKKKESRSGMEIMNLLQKEGRQRGTWNGKKTDNDFYKKI 221
           EMKKKEK+YIKLQERLNQVLMEKKKESRSGMEIMNLLQKEGRQRGTWNGKKTD DFYKKI
Sbjct: 121 EMKKKEKDYIKLQERLNQVLMEKKKESRSGMEIMNLLQKEGRQRGTWNGKKTDTDFYKKI 180

Query: 222 VDTYEVKNQELMSENADLKALLRSMQVDMRDFLNAPNGLPKQPLAVNERVESEGARFSFG 281
           VD YE KNQELM+EN  L+ALLRSMQ DMRDFLNAPNG     LA +E+ E++ ++   G
Sbjct: 181 VDAYEAKNQELMAENTSLRALLRSMQTDMRDFLNAPNG--SATLAGSEKREADPSQSPLG 240

Query: 282 GRTDVFDLPFHMARDKIEESLRNKMASIKEQMGELQDAQKESEVTSEATERELELEAQLV 341
           G+TDVFDLP+ MAR +IEESLR KMASIKE M +LQDA K + VTSEATERELELEAQLV
Sbjct: 241 GKTDVFDLPYRMARGQIEESLRTKMASIKESMVQLQDAPKRASVTSEATERELELEAQLV 300

Query: 342 EARSIILEQASIMSKHAKSERPRTQTIKLL 372
           EARSII EQ SIMSKH      R +++  L
Sbjct: 301 EARSIIQEQESIMSKHLPKSEQRRESVASL 328

BLAST of Carg08375 vs. TAIR 10
Match: AT2G18876.2 (Afadin/alpha-actinin-binding protein )

HSP 1 Score: 352.1 bits (902), Expect = 5.8e-97
Identity = 196/270 (72.59%), Postives = 229/270 (84.81%), Query Frame = 0

Query: 96  ISRLETKVERLESVIQAKDREIATITRTEAKAKAAFKAQIDKLQQEKEEFQRMVIGNQQV 155
           ++RLE KVERLE+ +QAK+RE+ ++TRTEAK  AA K Q +KLQ+E++EFQRMVI NQQV
Sbjct: 1   MARLEAKVERLETQLQAKERELGSVTRTEAKNTAALKTQNEKLQKERDEFQRMVIANQQV 60

Query: 156 RTQQMHEMKKKEKEYIKLQERLNQVLMEKKKESRSGMEIMNLLQKEGRQRGTWNGKKTDN 215
           +TQQ+HE KKKEKEYIKLQERLNQVLMEKKKE+RSGMEIMNLLQKEGRQRGTW+GKKTD+
Sbjct: 61  KTQQLHETKKKEKEYIKLQERLNQVLMEKKKETRSGMEIMNLLQKEGRQRGTWSGKKTDS 120

Query: 216 DFYKKIVDTYEVKNQELMSENADLKALLRSMQVDMRDFLNAPNGLPKQPLAVNERVESEG 275
           DFYKKIVD YE KNQELM+EN DL+ALLRS Q DMR FLNA  GL  Q L  N R  ++ 
Sbjct: 121 DFYKKIVDAYEAKNQELMAENTDLRALLRSTQGDMRSFLNASGGLTNQSLVANGRHGADP 180

Query: 276 ARFSFGGRTDVFDLPFHMARDKIEESLRNKMASIKEQMGELQDAQKESEVTSEATERELE 335
           ++   GG+TDVFDLPF MAR +IE+SLR+KM SIKE+MG+L DAQKE  +TSEA+ERELE
Sbjct: 181 SQSPLGGKTDVFDLPFRMARGQIEDSLRSKMVSIKERMGQLVDAQKEVSITSEASERELE 240

Query: 336 LEAQLVEARSIILEQASIMSKH-AKSERPR 365
           LEAQLVEARSII EQ SIMSKH  K++R R
Sbjct: 241 LEAQLVEARSIIQEQESIMSKHLPKTDRRR 270