Carg08352 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg08352
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionE3 ubiquitin-protein ligase XBAT32-like
LocationCarg_Chr05: 3603785 .. 3606736 (-)
RNA-Seq ExpressionCarg08352
SyntenyCarg08352
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAGCGCTAAACGGGCATGTTGACAGTGTGCAATTACTTTTGGACTTAGGTGCCTCTGCTTCTCAGGTTACTGTAGAAGATGGAACCACCATCGATCTAATAGGTGTGTCGCGTTTTTCGTTCCTGTTTTTTGTTTCTCTTATGCAGTTCTTAGTGAATTGAACAGTTTCTTTTCTTGTTTCTCATTACTGAAAAGCCTAATGTTGTTGAAGTATGTCATGTTCATTGGTTTTAACTGTGTTCTATTAGACTACTGAAGTGGAGATCATTCATTGCATGTTGTTACTGTCCTCAATCATGAAAATTGGTCTACCTGTTCCTTGCTGTATCAAGTTACATGTTGAGTTCAATAATGCGAATGTCAAACCTCTCTATGACCTCTTGGATATAATATTTTAACCAATTAAATTATGCTCAAGCTGTCATCTCTATGACTTCTTCGGGACCCAGTGTTCTTGTTGGCATACCGCCTCGTGTCCATCCTCCTTTGAAACCCAGCTTCCTCGCTGGCACATTACTTAGTGTCTGGCTCTGATACCATTTGTAATTGTCCAACCCCTTTCGGGCTTCCCCTTCAAGTTTTTAAAACGCGTTTGCTAGGGAGAGGTTTTCACACCCTTATAATGAATGCTTCGTTACGTGGGATCTCACAATTAGTCTGTTGGAAGCTGAAAAAGATTGATTCTTGAAGTTTCTTCTTAAGTTGGATGTTATTAGCCTAACGATGATTATGTTGTCATATTTAGGAGCTGGAAGCACAGCCCTTCATTACGCTGCATGCGGCGGGAGTGCCCAATGTTGTCAAGTAAGTTCGTGATTTAAAAACCATAGGAAATGATAAGATTTCGCAATTCGACCCATAAACATGGTTTCTGAATGTTTTGGTTCCAGATGTTGATAGCCAGGGGAGCCAGTCTTACCGCTCAAAATGCAAATGGGTATGTTTCTCTCAAGTATTTATGTTCAAATTCGTTTCTTTCGACGTCGCTGAAGATCGAATCCTTTTAAGAACTGTGGACCTGTACCGTGGCTTATGCATCTTAATAGCAATCAGTTTCTGTGGTTGATATTCATTTCTATTCTTTGTCGTTCTTATTCCTGATGAATTAGCGGAGCTTCCATCTCTGGCTAGGTGGTAGAATCTAGGCTAACTAAGTTTGTCTATGATTTGCACACCCCGAGTTTGAAGTAATTTCATTTCTATTCAGCAACAATAAGACAGGGTAACAGTGTAATATAATGTTACTCTTATCTTATTGAAGATCGACTCCCTTGATGGTTGCTCGTTCATGGCACCGAGACTGGCTCGAGGAAATTCTTAGCAAAGAACCAGAAGAAGCAGCCACAAATCTTGTCCCTTCACCATATTTGACCCTTCCCCTTATGAGCATTGTTAAAATTGCTAGGTGAGTCATTCTTGACAAAAATTATTAAAATAGCACAAATATGGTATAATAGACATGCCTTTGTTTTGTTCTAGCTTAGTTATGGATGTAGCTGATTTCAATATATCCAATAAGCTGCGTTTGTACTGTTCTTTTCCTCTTCGATCGATTGTGATTCATTAGAAATATAACGGTCTAAGCCCATCGCTAATAGATATTGTCTTATTTGGACTTTTCTTTTCGAGCTTCCCCCTCAAAGTTTTTAAAACGCGTATGCTTGTGAGAAGTTTCCACTCCTTTATAAAGAATCATTCGTCCTCCTCCCCAACTGATGTGAGATCTCACAATCCACCCCCCTTTTGAGGCCCCGTGTCCTTGCTGACACTTATTCCCTTCTCCAATTGATGTGGGACCCCCGAATCCACCCCTTTCGGGCCCAGCATCCTTGCTGGCACATCGCCTCGTGTCCACCCCCTTCGGGGCTCAGCCTCCTCGCTGACATCACTAGGTGTCTGGCTCTAATACCATTTGTAGTGGCTCAAACCCACCGCTAGTAGATATTATTCTTTTTAGGCTTTCCCTTTCGAGCTTTCTCTCAAGGTTTTTAAAATGCATGTGATAGAGGAAAATTTCCACACCCTTATACTTATAGAAAATGTTCTGTTCTCTTCCCCCGTCGACGTGGGGTCTCTTGTACAATGACTAACCTATGAACCCTTGATTTTTTTTCAGAGAATGTGGATGGAGAAGTTGTGACTCGTTACTTGCAAGCCAAGATCCATGTGTTGTCTGTTTGGAGAGAGAATGCACTGTTGCTGCACAAGGTAACTCAGTTATGATAATTATCCTCATTTATATGTCCTATTGTTAGCACCATATAATTCTCCAAGTTAAGCACATTATTTCAAACGTTGAAGGTTGTGATCATGAATTCTGCACAAGCTGTGCGCTATATCTCTGTTCTACAAACTGCACATCATCATGTTCCCATGGTCCACCCGGTTCAATCGCGTGCCCGCTCTGCCGAAATGGCATTGTTTCGTTCGTTAAACTTCCCAAAACAAAGCCAATTGCCAAAGAGATAGCCAGAACAAGTTTGTCTCTATCATTCTGCACTTGTTCTAGTGAGGTACCTGAACCACCTCACACATTAACCACCCCATTATGCAACCCTGAGTTCACTTGTTCCCAAATTTCCCCGCCCAAAGCATCGTGTCGACCCCTCAGCTCTCAAAGGTTTCCTTCAATGATACTCAACTCCAATCTTTGTATGGGAGCTCCTCACAGTAGCAGCTCTTCCTTGATACCTAGCAATGTTGACAGGAACACAAGGAACAATATTGAAGGTAGGAGTTCATGGTTTTCTGCCCTGAATCATGTAATGAATGGTTGCGGTTGCTGATAATTGTTTAAGGTGGAAACATTTTGTTTGAAGTTAAATTTTTCTCACATTATTCTTTTGATTAGCCCATCCATGTAAAAGAAGAGAATGCTCGAGTTTTGGTGATACTCACAAATGGGTTGTAGATATCCAATTTAAAATTCAAGGATATTCAAAGAAAAG

mRNA sequence

ATGGCAGCGCTAAACGGGCATGTTGACAGTGTGCAATTACTTTTGGACTTAGGTGCCTCTGCTTCTCAGGTTACTGTAGAAGATGGAACCACCATCGATCTAATAGGAGCTGGAAGCACAGCCCTTCATTACGCTGCATGCGGCGGGAGTGCCCAATGTTGTCAAATGTTGATAGCCAGGGGAGCCAGTCTTACCGCTCAAAATGCAAATGGATCGACTCCCTTGATGGTTGCTCGTTCATGGCACCGAGACTGGCTCGAGGAAATTCTTAGCAAAGAACCAGAAGAAGCAGCCACAAATCTTGTCCCTTCACCATATTTGACCCTTCCCCTTATGAGCATTGTTAAAATTGCTAGAGAATGTGGATGGAGAAGTTGTGACTCGTTACTTGCAAGCCAAGATCCATGTGTTGTCTGTTTGGAGAGAGAATGCACTGTTGCTGCACAAGGTTGTGATCATGAATTCTGCACAAGCTGTGCGCTATATCTCTGTTCTACAAACTGCACATCATCATGTTCCCATGGTCCACCCGGTTCAATCGCGTGCCCGCTCTGCCGAAATGGCATTGTTTCGTTCGTTAAACTTCCCAAAACAAAGCCAATTGCCAAAGAGATAGCCAGAACAAGTTTGTCTCTATCATTCTGCACTTGTTCTAGTGAGGTACCTGAACCACCTCACACATTAACCACCCCATTATGCAACCCTGAGTTCACTTGTTCCCAAATTTCCCCGCCCAAAGCATCGTGTCGACCCCTCAGCTCTCAAAGGTTTCCTTCAATGATACTCAACTCCAATCTTTGTATGGGAGCTCCTCACAGTAGCAGCTCTTCCTTGATACCTAGCAATGTTGACAGGAACACAAGGAACAATATTGAAGGTAGGAGTTCATGGTTTTCTGCCCTGAATCATGTAATGAATGGTTGCGGTTGCTGATAATTGTTTAAGGTGGAAACATTTTGTTTGAAGTTAAATTTTTCTCACATTATTCTTTTGATTAGCCCATCCATGTAAAAGAAGAGAATGCTCGAGTTTTGGTGATACTCACAAATGGGTTGTAGATATCCAATTTAAAATTCAAGGATATTCAAAGAAAAG

Coding sequence (CDS)

ATGGCAGCGCTAAACGGGCATGTTGACAGTGTGCAATTACTTTTGGACTTAGGTGCCTCTGCTTCTCAGGTTACTGTAGAAGATGGAACCACCATCGATCTAATAGGAGCTGGAAGCACAGCCCTTCATTACGCTGCATGCGGCGGGAGTGCCCAATGTTGTCAAATGTTGATAGCCAGGGGAGCCAGTCTTACCGCTCAAAATGCAAATGGATCGACTCCCTTGATGGTTGCTCGTTCATGGCACCGAGACTGGCTCGAGGAAATTCTTAGCAAAGAACCAGAAGAAGCAGCCACAAATCTTGTCCCTTCACCATATTTGACCCTTCCCCTTATGAGCATTGTTAAAATTGCTAGAGAATGTGGATGGAGAAGTTGTGACTCGTTACTTGCAAGCCAAGATCCATGTGTTGTCTGTTTGGAGAGAGAATGCACTGTTGCTGCACAAGGTTGTGATCATGAATTCTGCACAAGCTGTGCGCTATATCTCTGTTCTACAAACTGCACATCATCATGTTCCCATGGTCCACCCGGTTCAATCGCGTGCCCGCTCTGCCGAAATGGCATTGTTTCGTTCGTTAAACTTCCCAAAACAAAGCCAATTGCCAAAGAGATAGCCAGAACAAGTTTGTCTCTATCATTCTGCACTTGTTCTAGTGAGGTACCTGAACCACCTCACACATTAACCACCCCATTATGCAACCCTGAGTTCACTTGTTCCCAAATTTCCCCGCCCAAAGCATCGTGTCGACCCCTCAGCTCTCAAAGGTTTCCTTCAATGATACTCAACTCCAATCTTTGTATGGGAGCTCCTCACAGTAGCAGCTCTTCCTTGATACCTAGCAATGTTGACAGGAACACAAGGAACAATATTGAAGGTAGGAGTTCATGGTTTTCTGCCCTGAATCATGTAATGAATGGTTGCGGTTGCTGA

Protein sequence

MAALNGHVDSVQLLLDLGASASQVTVEDGTTIDLIGAGSTALHYAACGGSAQCCQMLIARGASLTAQNANGSTPLMVARSWHRDWLEEILSKEPEEAATNLVPSPYLTLPLMSIVKIARECGWRSCDSLLASQDPCVVCLERECTVAAQGCDHEFCTSCALYLCSTNCTSSCSHGPPGSIACPLCRNGIVSFVKLPKTKPIAKEIARTSLSLSFCTCSSEVPEPPHTLTTPLCNPEFTCSQISPPKASCRPLSSQRFPSMILNSNLCMGAPHSSSSSLIPSNVDRNTRNNIEGRSSWFSALNHVMNGCGC
Homology
BLAST of Carg08352 vs. NCBI nr
Match: KAG7029787.1 (putative E3 ubiquitin-protein ligase XBOS32, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 630.9 bits (1626), Expect = 5.6e-177
Identity = 310/310 (100.00%), Postives = 310/310 (100.00%), Query Frame = 0

Query: 1   MAALNGHVDSVQLLLDLGASASQVTVEDGTTIDLIGAGSTALHYAACGGSAQCCQMLIAR 60
           MAALNGHVDSVQLLLDLGASASQVTVEDGTTIDLIGAGSTALHYAACGGSAQCCQMLIAR
Sbjct: 1   MAALNGHVDSVQLLLDLGASASQVTVEDGTTIDLIGAGSTALHYAACGGSAQCCQMLIAR 60

Query: 61  GASLTAQNANGSTPLMVARSWHRDWLEEILSKEPEEAATNLVPSPYLTLPLMSIVKIARE 120
           GASLTAQNANGSTPLMVARSWHRDWLEEILSKEPEEAATNLVPSPYLTLPLMSIVKIARE
Sbjct: 61  GASLTAQNANGSTPLMVARSWHRDWLEEILSKEPEEAATNLVPSPYLTLPLMSIVKIARE 120

Query: 121 CGWRSCDSLLASQDPCVVCLERECTVAAQGCDHEFCTSCALYLCSTNCTSSCSHGPPGSI 180
           CGWRSCDSLLASQDPCVVCLERECTVAAQGCDHEFCTSCALYLCSTNCTSSCSHGPPGSI
Sbjct: 121 CGWRSCDSLLASQDPCVVCLERECTVAAQGCDHEFCTSCALYLCSTNCTSSCSHGPPGSI 180

Query: 181 ACPLCRNGIVSFVKLPKTKPIAKEIARTSLSLSFCTCSSEVPEPPHTLTTPLCNPEFTCS 240
           ACPLCRNGIVSFVKLPKTKPIAKEIARTSLSLSFCTCSSEVPEPPHTLTTPLCNPEFTCS
Sbjct: 181 ACPLCRNGIVSFVKLPKTKPIAKEIARTSLSLSFCTCSSEVPEPPHTLTTPLCNPEFTCS 240

Query: 241 QISPPKASCRPLSSQRFPSMILNSNLCMGAPHSSSSSLIPSNVDRNTRNNIEGRSSWFSA 300
           QISPPKASCRPLSSQRFPSMILNSNLCMGAPHSSSSSLIPSNVDRNTRNNIEGRSSWFSA
Sbjct: 241 QISPPKASCRPLSSQRFPSMILNSNLCMGAPHSSSSSLIPSNVDRNTRNNIEGRSSWFSA 300

Query: 301 LNHVMNGCGC 311
           LNHVMNGCGC
Sbjct: 301 LNHVMNGCGC 310

BLAST of Carg08352 vs. NCBI nr
Match: KAG6598844.1 (E3 ubiquitin-protein ligase XBAT32, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 629.0 bits (1621), Expect = 2.1e-176
Identity = 309/310 (99.68%), Postives = 309/310 (99.68%), Query Frame = 0

Query: 1   MAALNGHVDSVQLLLDLGASASQVTVEDGTTIDLIGAGSTALHYAACGGSAQCCQMLIAR 60
           MAALNGHVDSVQLLLDLGASASQVTVEDGTTIDLIGAGSTALHYAACGGSAQCCQMLIAR
Sbjct: 181 MAALNGHVDSVQLLLDLGASASQVTVEDGTTIDLIGAGSTALHYAACGGSAQCCQMLIAR 240

Query: 61  GASLTAQNANGSTPLMVARSWHRDWLEEILSKEPEEAATNLVPSPYLTLPLMSIVKIARE 120
           GASLTAQNANGSTPLMVARSWHRDWLEEILSKEPEEAATNLVPSPYLTLPLMSIVKIARE
Sbjct: 241 GASLTAQNANGSTPLMVARSWHRDWLEEILSKEPEEAATNLVPSPYLTLPLMSIVKIARE 300

Query: 121 CGWRSCDSLLASQDPCVVCLERECTVAAQGCDHEFCTSCALYLCSTNCTSSCSHGPPGSI 180
           CGWRSCDSLLASQDPCVVCLERECTVAAQGCDHEFCTSCALYLCSTNCTSSCSHGPPGSI
Sbjct: 301 CGWRSCDSLLASQDPCVVCLERECTVAAQGCDHEFCTSCALYLCSTNCTSSCSHGPPGSI 360

Query: 181 ACPLCRNGIVSFVKLPKTKPIAKEIARTSLSLSFCTCSSEVPEPPHTLTTPLCNPEFTCS 240
           ACPLCRNGIVSFVKLPKTKPIAKEIARTSLSLSFCTCSSEVPEPPHTLTTPLCNPEFTCS
Sbjct: 361 ACPLCRNGIVSFVKLPKTKPIAKEIARTSLSLSFCTCSSEVPEPPHTLTTPLCNPEFTCS 420

Query: 241 QISPPKASCRPLSSQRFPSMILNSNLCMGAPHSSSSSLIPSNVDRNTRNNIEGRSSWFSA 300
           QISPPKASCRPLSSQRFPSMILNSNLCMGAPHS SSSLIPSNVDRNTRNNIEGRSSWFSA
Sbjct: 421 QISPPKASCRPLSSQRFPSMILNSNLCMGAPHSRSSSLIPSNVDRNTRNNIEGRSSWFSA 480

Query: 301 LNHVMNGCGC 311
           LNHVMNGCGC
Sbjct: 481 LNHVMNGCGC 490

BLAST of Carg08352 vs. NCBI nr
Match: XP_022929749.1 (E3 ubiquitin-protein ligase XBAT32-like [Cucurbita moschata])

HSP 1 Score: 614.8 bits (1584), Expect = 4.2e-172
Identity = 302/310 (97.42%), Postives = 306/310 (98.71%), Query Frame = 0

Query: 1   MAALNGHVDSVQLLLDLGASASQVTVEDGTTIDLIGAGSTALHYAACGGSAQCCQMLIAR 60
           MAALNGHVDSVQLLLDLGASASQVTVEDGTTIDLIGAGSTALHYAACGGSAQCCQMLIAR
Sbjct: 181 MAALNGHVDSVQLLLDLGASASQVTVEDGTTIDLIGAGSTALHYAACGGSAQCCQMLIAR 240

Query: 61  GASLTAQNANGSTPLMVARSWHRDWLEEILSKEPEEAATNLVPSPYLTLPLMSIVKIARE 120
           GASLTAQNANGSTPLMVARSWHRDWLEEILSKEPEEAATNLVPSPYLTLPLMSIVKIARE
Sbjct: 241 GASLTAQNANGSTPLMVARSWHRDWLEEILSKEPEEAATNLVPSPYLTLPLMSIVKIARE 300

Query: 121 CGWRSCDSLLASQDPCVVCLERECTVAAQGCDHEFCTSCALYLCSTNCTSSCSHGPPGSI 180
           CGW+SCDSLLASQDPCVVCLERECTVAAQGCDHEFCTSCALYLCSTNCTSSCSHGPPGSI
Sbjct: 301 CGWKSCDSLLASQDPCVVCLERECTVAAQGCDHEFCTSCALYLCSTNCTSSCSHGPPGSI 360

Query: 181 ACPLCRNGIVSFVKLPKTKPIAKEIARTSLSLSFCTCSSEVPEPPHTLTTPLCNPEFTCS 240
           ACPLCRNGIVSFVKLPKTKPIAKEIARTSLSLSFCTCSS VPEPP TLTTPLCNPEFTCS
Sbjct: 361 ACPLCRNGIVSFVKLPKTKPIAKEIARTSLSLSFCTCSSGVPEPPPTLTTPLCNPEFTCS 420

Query: 241 QISPPKASCRPLSSQRFPSMILNSNLCMGAPHSSSSSLIPSNVDRNTRNNIEGRSSWFSA 300
           QISPP+A CRPLSSQRFPS+ILNSNLC+GAPHSSSSSLIPSNVDRN RNNIEGRSSWFSA
Sbjct: 421 QISPPEAPCRPLSSQRFPSVILNSNLCLGAPHSSSSSLIPSNVDRNIRNNIEGRSSWFSA 480

Query: 301 LNHVMNGCGC 311
           LNHVMNGCGC
Sbjct: 481 LNHVMNGCGC 490

BLAST of Carg08352 vs. NCBI nr
Match: XP_023547016.1 (E3 ubiquitin-protein ligase XBAT32-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 613.6 bits (1581), Expect = 9.3e-172
Identity = 302/310 (97.42%), Postives = 306/310 (98.71%), Query Frame = 0

Query: 1   MAALNGHVDSVQLLLDLGASASQVTVEDGTTIDLIGAGSTALHYAACGGSAQCCQMLIAR 60
           MAALNGHV SVQLLLDLGASASQVTVEDGTTIDLIGAGSTALHYAACGGSAQCCQMLIAR
Sbjct: 181 MAALNGHVGSVQLLLDLGASASQVTVEDGTTIDLIGAGSTALHYAACGGSAQCCQMLIAR 240

Query: 61  GASLTAQNANGSTPLMVARSWHRDWLEEILSKEPEEAATNLVPSPYLTLPLMSIVKIARE 120
           GASLTAQNANG TPLMVARSWHRDWLEEILSKEPEEAATNLVPSPYLTLPLMSIVKIARE
Sbjct: 241 GASLTAQNANGWTPLMVARSWHRDWLEEILSKEPEEAATNLVPSPYLTLPLMSIVKIARE 300

Query: 121 CGWRSCDSLLASQDPCVVCLERECTVAAQGCDHEFCTSCALYLCSTNCTSSCSHGPPGSI 180
           CGWRSCDSLLASQDPCVVCLERECTVAAQGCDHEFCTSCALYLCSTNCTSSCS+GPPGSI
Sbjct: 301 CGWRSCDSLLASQDPCVVCLERECTVAAQGCDHEFCTSCALYLCSTNCTSSCSNGPPGSI 360

Query: 181 ACPLCRNGIVSFVKLPKTKPIAKEIARTSLSLSFCTCSSEVPEPPHTLTTPLCNPEFTCS 240
           ACPLCRNGIVSF+KLPKTKPIAKEIARTSLSLSFCTCSSEVPEPP TLTTPLCNPEFTCS
Sbjct: 361 ACPLCRNGIVSFIKLPKTKPIAKEIARTSLSLSFCTCSSEVPEPPPTLTTPLCNPEFTCS 420

Query: 241 QISPPKASCRPLSSQRFPSMILNSNLCMGAPHSSSSSLIPSNVDRNTRNNIEGRSSWFSA 300
           Q+SPP ASC+PLSSQRFPSMILNSNLCMGAPHSSSSSLIPSNVDRNTRNNIEGRSSWFSA
Sbjct: 421 QLSPPGASCQPLSSQRFPSMILNSNLCMGAPHSSSSSLIPSNVDRNTRNNIEGRSSWFSA 480

Query: 301 LNHVMNGCGC 311
           LNHVMNGCGC
Sbjct: 481 LNHVMNGCGC 490

BLAST of Carg08352 vs. NCBI nr
Match: XP_022997410.1 (E3 ubiquitin-protein ligase XBAT32-like [Cucurbita maxima])

HSP 1 Score: 606.3 bits (1562), Expect = 1.5e-169
Identity = 299/310 (96.45%), Postives = 302/310 (97.42%), Query Frame = 0

Query: 1   MAALNGHVDSVQLLLDLGASASQVTVEDGTTIDLIGAGSTALHYAACGGSAQCCQMLIAR 60
           MAALNGHVDSVQLLLDLGASASQVTVEDGTTIDLIGAGSTALHYAACGGSAQCCQML+AR
Sbjct: 181 MAALNGHVDSVQLLLDLGASASQVTVEDGTTIDLIGAGSTALHYAACGGSAQCCQMLLAR 240

Query: 61  GASLTAQNANGSTPLMVARSWHRDWLEEILSKEPEEAATNLVPSPYLTLPLMSIVKIARE 120
           GASLT QNANG TPLMVARSWHRDWLEEILSKEPEEAATNLVPSPYLTLPLMSIVKIARE
Sbjct: 241 GASLTVQNANGWTPLMVARSWHRDWLEEILSKEPEEAATNLVPSPYLTLPLMSIVKIARE 300

Query: 121 CGWRSCDSLLASQDPCVVCLERECTVAAQGCDHEFCTSCALYLCSTNCTSSCSHGPPGSI 180
           CGWRSCDSLLASQDPCVVCLERECTVAAQGC+HEFCTSCALYLCSTNCTSSCSHGPPGSI
Sbjct: 301 CGWRSCDSLLASQDPCVVCLERECTVAAQGCEHEFCTSCALYLCSTNCTSSCSHGPPGSI 360

Query: 181 ACPLCRNGIVSFVKLPKTKPIAKEIARTSLSLSFCTCSSEVPEPPHTLTTPLCNPEFTCS 240
           ACPLCRNGIVSFVKLPKTKPIAKEIARTSLSLSFCTCSSEVPEPP TLTTPL NPEFTCS
Sbjct: 361 ACPLCRNGIVSFVKLPKTKPIAKEIARTSLSLSFCTCSSEVPEPPPTLTTPLYNPEFTCS 420

Query: 241 QISPPKASCRPLSSQRFPSMILNSNLCMGAPHSSSSSLIPSNVDRNTRNNIEGRSSWFSA 300
           QISPP ASCRPL+ QRFPSMILNSN CMGAPH SSSSLIPSNVDRNTRNNIEGRSSWFSA
Sbjct: 421 QISPPGASCRPLNCQRFPSMILNSNRCMGAPHRSSSSLIPSNVDRNTRNNIEGRSSWFSA 480

Query: 301 LNHVMNGCGC 311
           LNHVMNGCGC
Sbjct: 481 LNHVMNGCGC 490

BLAST of Carg08352 vs. ExPASy Swiss-Prot
Match: Q6NLQ8 (E3 ubiquitin-protein ligase XBAT32 OS=Arabidopsis thaliana OX=3702 GN=XBAT32 PE=1 SV=1)

HSP 1 Score: 331.6 bits (849), Expect = 9.3e-90
Identity = 184/327 (56.27%), Postives = 227/327 (69.42%), Query Frame = 0

Query: 1   MAALNGHVDSVQLLLDLGASASQVTVEDGTTIDLIGAGSTALHYAACGGSAQCCQMLIAR 60
           +AALNGH+++VQLLLDLGAS +QVTVEDGTTIDLIGAGSTALHYA+CGG+ QCCQ+LI++
Sbjct: 184 VAALNGHIETVQLLLDLGASVTQVTVEDGTTIDLIGAGSTALHYASCGGNTQCCQLLISK 243

Query: 61  GASLTAQNANGSTPLMVARSWHRDWLEEILSKEPEEAATNL--VPSPYLTLPLMSIVKIA 120
           GA L A N+NG TP+MVARSWHR+WLEEIL+   E+   +L  VPSP+L LPLMSIV IA
Sbjct: 244 GACLAAVNSNGWTPMMVARSWHRNWLEEILNPTTEQPQLHLPNVPSPFLCLPLMSIVNIA 303

Query: 121 RECGWRSCDSLLASQDPCVVCLERECTVAAQGCDHEFCTSCALYLCSTNCTSS-CSHGPP 180
           +ECGWR  D L   +DPC VCLER+CTVAA GC HEFCT+CALYL +T+ TSS  S+  P
Sbjct: 304 QECGWRENDCLTPCRDPCAVCLERKCTVAADGCAHEFCTNCALYLSTTSITSSKTSNVTP 363

Query: 181 GSIACPLCRNGIVSFVKLPKTKPIAK--EIARTSLSLSFCTCSSEVPEPPHTLTTPLCNP 240
           GS+ CPLCRNGIVSF KLP T    +    +RTS+SLSFCTCSS+V +     T  L NP
Sbjct: 364 GSVPCPLCRNGIVSFTKLPHTTATTRTSTSSRTSISLSFCTCSSDVLD-----TALLTNP 423

Query: 241 EFTCSQI----------SPPKASCRPLSSQRFPSMILNSNLCMGAPHSS--SSSLIPSNV 300
            ++C  +          S   ++ R LS +RFP  +      +  P S     S   S V
Sbjct: 424 HYSCKPVVSRTGSRTPQSARSSAFRSLSCRRFPPSLCLGGSDVDEPRSRLIGGSYSRSGV 483

Query: 301 D-RNTRNNIEGRSSWFSALNHVMNGCG 310
             R + + +EG+ SWFSALNH +   G
Sbjct: 484 GFRRSTSQVEGKRSWFSALNHCVTTGG 505

BLAST of Carg08352 vs. ExPASy Swiss-Prot
Match: Q6KAE5 (Probable E3 ubiquitin-protein ligase XBOS32 OS=Oryza sativa subsp. japonica OX=39947 GN=XBOS32 PE=2 SV=2)

HSP 1 Score: 314.7 bits (805), Expect = 1.2e-84
Identity = 171/272 (62.87%), Postives = 202/272 (74.26%), Query Frame = 0

Query: 1   MAALNGHVDSVQLLLDLGASASQVTVEDGTTIDLIGAGSTALHYAACGGSAQCCQMLIAR 60
           MAALNGHV+ VQLLLDLGAS  + T+EDGTTIDLIGAGST LHYAACGG+A CCQ+LIAR
Sbjct: 187 MAALNGHVECVQLLLDLGASVIEATIEDGTTIDLIGAGSTPLHYAACGGNAVCCQLLIAR 246

Query: 61  GASLTAQNANGSTPLMVARSWHRDWLEEILSKEPEEAATNLVPSPYLTLPLMSIVKIARE 120
           GASL+AQNA+G TPLMVARSWHR+ LEEILSKEP E+    VPSPYL LPLMSI+ IARE
Sbjct: 247 GASLSAQNASGWTPLMVARSWHRNSLEEILSKEP-ESRIRTVPSPYLCLPLMSIMSIARE 306

Query: 121 CGWRSCDSLLASQDPCVVCLERECTVAAQGCDHEFCTSCALYLCSTNCTSSCSHGPPGSI 180
            GWR  +      DPC VCLE  C+VAA+GC HEFCT CALYLCST+ TS     P G+I
Sbjct: 307 FGWRYLNQSPVCIDPCAVCLEGSCSVAAEGCKHEFCTRCALYLCSTSYTSV---SPAGAI 366

Query: 181 ACPLCRNGIVSFVKLPKTKPIAKEIARTSLSLSFC-TCSSEVPEPPHTLTTPLCNPEFTC 240
            CPLCR+ I++F  LP T PI +E+ R SLSLSFC TC +   +   ++ + L   EF C
Sbjct: 367 PCPLCRHPIIAFTALPGTSPI-RELPRNSLSLSFCTTCPAVNSDSTPSIASHLYRTEFQC 426

Query: 241 SQISP-PKASCRPLSSQRFPSMILNSNLCMGA 271
           +++ P   +S R LS QR P+M LN + CMGA
Sbjct: 427 ARMPPMGSSSFRSLSCQRLPAMKLNPSFCMGA 453

BLAST of Carg08352 vs. ExPASy Swiss-Prot
Match: Q7EZ44 (Probable E3 ubiquitin-protein ligase XBOS35 OS=Oryza sativa subsp. japonica OX=39947 GN=XBOS35 PE=2 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 1.0e-72
Identity = 136/219 (62.10%), Postives = 166/219 (75.80%), Query Frame = 0

Query: 1   MAALNGHVDSVQLLLDLGASASQVTVEDGTTIDLIGAGSTALHYAACGGSAQCCQMLIAR 60
           +AAL+GH + VQLLLDLGAS S+VT+ DG+TIDLIG+GST LHYAACGGSA CCQ+L+A 
Sbjct: 187 LAALHGHAECVQLLLDLGASVSEVTINDGSTIDLIGSGSTPLHYAACGGSAVCCQLLVAA 246

Query: 61  GASLTAQNANGSTPLMVARSWHRDWLEEILSKEPEEAATNLVPSPYLTLPLMSIVKIARE 120
           GA++ AQN NG TPLMVARSWH+  +E IL+K   E    ++PS YL+LPLMSIVKIARE
Sbjct: 247 GANMRAQNTNGLTPLMVARSWHKSSVEGILTKR-SEVPVRILPSSYLSLPLMSIVKIARE 306

Query: 121 CGWRSCDSLLASQDPCVVCLERECTVAAQGCDHEFCTSCALYLCSTNCTSSCSHGPPGSI 180
           CGWR         DPC +CL+ ECTV+A+GC HEFCT CALYLC+T  +S+   G PGSI
Sbjct: 307 CGWRKTSVSSVCHDPCAICLDTECTVSAEGCGHEFCTKCALYLCATASSSTSIRGVPGSI 366

Query: 181 ACPLCRNGIVSFVKLPKTKPIAKEIARTSLSLSFCTCSS 220
            CPLCR+ IVSFV+L  T PI KE+  T+ SL+ C   +
Sbjct: 367 PCPLCRHTIVSFVRLTSTTPI-KELPWTNKSLALCAAGA 403

BLAST of Carg08352 vs. ExPASy Swiss-Prot
Match: Q337A0 (Probable E3 ubiquitin-protein ligase XBOS33 OS=Oryza sativa subsp. japonica OX=39947 GN=XBOS33 PE=2 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 5.1e-56
Identity = 130/271 (47.97%), Postives = 168/271 (61.99%), Query Frame = 0

Query: 1   MAALNGHVDSVQLLLDLGASASQVTVEDGTTIDLIGAGSTALHYAACGGSAQCCQMLIAR 60
           MAALNGH D +QLL+DLGA+ S VT   GTT +LIGAGST LHYAA GG+A+CCQ+L+++
Sbjct: 192 MAALNGHFDCMQLLIDLGANVSAVTFPYGTTANLIGAGSTPLHYAAGGGNAECCQLLLSK 251

Query: 61  GASLTAQNANGSTPLMVARSWHRDWLEEILSKEPEEAATNLVPSPYLTLPLMSIVKIARE 120
           GAS    N NG  P+ VAR + R +LE +LS     +     PS YL LP MSI+ IARE
Sbjct: 252 GASKLTLNCNGWLPIDVARMFGRRFLEPLLSPNSNSSIPAYQPSNYLALPFMSILNIARE 311

Query: 121 CGWRSCDSLLASQDPCVVCLERECTVAAQGCDHEFCTSCALYLCSTNCTSSCSHGPPGSI 180
            G     + +   D C VCLER C+VAA+GC HEFC  CALYLCST+ T     GPPGSI
Sbjct: 312 FGLLHTVASVDDSDLCAVCLERSCSVAAEGCCHEFCIKCALYLCSTSNTRVEFTGPPGSI 371

Query: 181 ACPLCRNGIVSFVKLPKTKPIAKEIARTSLSLSFCT---CSSEVPEPPHTLTTPLCNPEF 240
            CPLCRNGI+SF KLP T     E  ++S +L+FC     ++   + P T++      E 
Sbjct: 372 PCPLCRNGIMSFTKLPST---PTEGLKSSSALTFCNPCMLNTRSVDSPATIS----KAEI 431

Query: 241 TCSQISPPKAS-CRPLSSQRFPSMILNSNLC 268
             ++++   +    PL+   FPS  L +  C
Sbjct: 432 RRNRVAAVSSELVCPLTCSPFPSSALPTCRC 455

BLAST of Carg08352 vs. ExPASy Swiss-Prot
Match: Q4FE45 (E3 ubiquitin-protein ligase XBAT33 OS=Arabidopsis thaliana OX=3702 GN=XBAT33 PE=2 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 2.5e-55
Identity = 128/277 (46.21%), Postives = 163/277 (58.84%), Query Frame = 0

Query: 1   MAALNGHVDSVQLLLDLGASASQVTVEDGTTIDLIGAGSTALHYAACGGSAQCCQMLIAR 60
           MAALNG  D VQLLLDL A+ S VT   GT++D+IGAGST LHYAACGG+ +CCQ+L+AR
Sbjct: 178 MAALNGLFDCVQLLLDLEANVSAVTFHYGTSMDMIGAGSTPLHYAACGGNLKCCQILLAR 237

Query: 61  GASLTAQNANGSTPLMVARSWHRDWLEEILSKEPEEAATNLVPSPYLTLPLMSIVKIARE 120
           GA     N NG  P+ +AR W R WLE +LS   +        S YL+LPL+SI+ IARE
Sbjct: 238 GARKMTLNCNGWLPIDIARMWSRHWLEPLLSPNSDVVIPAFPHSNYLSLPLLSILNIARE 297

Query: 121 CGWRSCDSLLASQDPCVVCLERECTVAAQGCDHEFCTSCALYLCSTNCTSSCSHGPPGSI 180
            G +S  ++    D C VCLER CTVAA+GC+H+ C  CALYLCS++   S + GPPGSI
Sbjct: 298 FGLQSA-TIGDEVDICAVCLERTCTVAAEGCEHQLCVRCALYLCSSSNVPSVTVGPPGSI 357

Query: 181 ACPLCRNGIVSFVKLPKTKPIAKEIARTSLSLSFC----------TCSSEVPEPPHTLTT 240
            CPLCR+GI +F +LP +  + +E+ +  +SL FC          T  S    PP    +
Sbjct: 358 PCPLCRHGITAFKRLPSS--LTREM-KLPMSLGFCAPCMLHTGDTTDQSSPTCPPTEQRS 417

Query: 241 PLCNPEFTCSQISPPKASCRPLSSQRFPSMILNSNLC 268
                    S I  P  +C P  S   P    N   C
Sbjct: 418 SKTRAASVSSDIFCP-VTCSPFPSVNIPMCTCNEGTC 449

BLAST of Carg08352 vs. ExPASy TrEMBL
Match: A0A6J1EPN8 (E3 ubiquitin-protein ligase XBAT32-like OS=Cucurbita moschata OX=3662 GN=LOC111436242 PE=4 SV=1)

HSP 1 Score: 614.8 bits (1584), Expect = 2.0e-172
Identity = 302/310 (97.42%), Postives = 306/310 (98.71%), Query Frame = 0

Query: 1   MAALNGHVDSVQLLLDLGASASQVTVEDGTTIDLIGAGSTALHYAACGGSAQCCQMLIAR 60
           MAALNGHVDSVQLLLDLGASASQVTVEDGTTIDLIGAGSTALHYAACGGSAQCCQMLIAR
Sbjct: 181 MAALNGHVDSVQLLLDLGASASQVTVEDGTTIDLIGAGSTALHYAACGGSAQCCQMLIAR 240

Query: 61  GASLTAQNANGSTPLMVARSWHRDWLEEILSKEPEEAATNLVPSPYLTLPLMSIVKIARE 120
           GASLTAQNANGSTPLMVARSWHRDWLEEILSKEPEEAATNLVPSPYLTLPLMSIVKIARE
Sbjct: 241 GASLTAQNANGSTPLMVARSWHRDWLEEILSKEPEEAATNLVPSPYLTLPLMSIVKIARE 300

Query: 121 CGWRSCDSLLASQDPCVVCLERECTVAAQGCDHEFCTSCALYLCSTNCTSSCSHGPPGSI 180
           CGW+SCDSLLASQDPCVVCLERECTVAAQGCDHEFCTSCALYLCSTNCTSSCSHGPPGSI
Sbjct: 301 CGWKSCDSLLASQDPCVVCLERECTVAAQGCDHEFCTSCALYLCSTNCTSSCSHGPPGSI 360

Query: 181 ACPLCRNGIVSFVKLPKTKPIAKEIARTSLSLSFCTCSSEVPEPPHTLTTPLCNPEFTCS 240
           ACPLCRNGIVSFVKLPKTKPIAKEIARTSLSLSFCTCSS VPEPP TLTTPLCNPEFTCS
Sbjct: 361 ACPLCRNGIVSFVKLPKTKPIAKEIARTSLSLSFCTCSSGVPEPPPTLTTPLCNPEFTCS 420

Query: 241 QISPPKASCRPLSSQRFPSMILNSNLCMGAPHSSSSSLIPSNVDRNTRNNIEGRSSWFSA 300
           QISPP+A CRPLSSQRFPS+ILNSNLC+GAPHSSSSSLIPSNVDRN RNNIEGRSSWFSA
Sbjct: 421 QISPPEAPCRPLSSQRFPSVILNSNLCLGAPHSSSSSLIPSNVDRNIRNNIEGRSSWFSA 480

Query: 301 LNHVMNGCGC 311
           LNHVMNGCGC
Sbjct: 481 LNHVMNGCGC 490

BLAST of Carg08352 vs. ExPASy TrEMBL
Match: A0A6J1K9I9 (E3 ubiquitin-protein ligase XBAT32-like OS=Cucurbita maxima OX=3661 GN=LOC111492335 PE=4 SV=1)

HSP 1 Score: 606.3 bits (1562), Expect = 7.2e-170
Identity = 299/310 (96.45%), Postives = 302/310 (97.42%), Query Frame = 0

Query: 1   MAALNGHVDSVQLLLDLGASASQVTVEDGTTIDLIGAGSTALHYAACGGSAQCCQMLIAR 60
           MAALNGHVDSVQLLLDLGASASQVTVEDGTTIDLIGAGSTALHYAACGGSAQCCQML+AR
Sbjct: 181 MAALNGHVDSVQLLLDLGASASQVTVEDGTTIDLIGAGSTALHYAACGGSAQCCQMLLAR 240

Query: 61  GASLTAQNANGSTPLMVARSWHRDWLEEILSKEPEEAATNLVPSPYLTLPLMSIVKIARE 120
           GASLT QNANG TPLMVARSWHRDWLEEILSKEPEEAATNLVPSPYLTLPLMSIVKIARE
Sbjct: 241 GASLTVQNANGWTPLMVARSWHRDWLEEILSKEPEEAATNLVPSPYLTLPLMSIVKIARE 300

Query: 121 CGWRSCDSLLASQDPCVVCLERECTVAAQGCDHEFCTSCALYLCSTNCTSSCSHGPPGSI 180
           CGWRSCDSLLASQDPCVVCLERECTVAAQGC+HEFCTSCALYLCSTNCTSSCSHGPPGSI
Sbjct: 301 CGWRSCDSLLASQDPCVVCLERECTVAAQGCEHEFCTSCALYLCSTNCTSSCSHGPPGSI 360

Query: 181 ACPLCRNGIVSFVKLPKTKPIAKEIARTSLSLSFCTCSSEVPEPPHTLTTPLCNPEFTCS 240
           ACPLCRNGIVSFVKLPKTKPIAKEIARTSLSLSFCTCSSEVPEPP TLTTPL NPEFTCS
Sbjct: 361 ACPLCRNGIVSFVKLPKTKPIAKEIARTSLSLSFCTCSSEVPEPPPTLTTPLYNPEFTCS 420

Query: 241 QISPPKASCRPLSSQRFPSMILNSNLCMGAPHSSSSSLIPSNVDRNTRNNIEGRSSWFSA 300
           QISPP ASCRPL+ QRFPSMILNSN CMGAPH SSSSLIPSNVDRNTRNNIEGRSSWFSA
Sbjct: 421 QISPPGASCRPLNCQRFPSMILNSNRCMGAPHRSSSSLIPSNVDRNTRNNIEGRSSWFSA 480

Query: 301 LNHVMNGCGC 311
           LNHVMNGCGC
Sbjct: 481 LNHVMNGCGC 490

BLAST of Carg08352 vs. ExPASy TrEMBL
Match: A0A5A7U2Y7 (Putative E3 ubiquitin-protein ligase XBOS32 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold231G00070 PE=4 SV=1)

HSP 1 Score: 521.2 bits (1341), Expect = 3.0e-144
Identity = 269/325 (82.77%), Postives = 279/325 (85.85%), Query Frame = 0

Query: 1   MAALNGHVDSVQLLLDLGASASQVTVEDGTTIDLIGAGSTALHYAACGGSAQCCQMLIAR 60
           MAALNGHVDS+QLLLDLGASA QVTVEDGTTIDLIGAGSTALHYAACGG+AQCCQMLIAR
Sbjct: 187 MAALNGHVDSLQLLLDLGASAFQVTVEDGTTIDLIGAGSTALHYAACGGNAQCCQMLIAR 246

Query: 61  GASLTAQNANGSTPLMVARSWHRDWLEEILSKEPEEAATNLVPSPYLTLPLMSIVKIARE 120
           GA LTAQNANG TPLMVARSWHRDWLEEILS+E    ATNLVPSPYLTLPLMSIV+IARE
Sbjct: 247 GADLTAQNANGWTPLMVARSWHRDWLEEILSREAGAVATNLVPSPYLTLPLMSIVRIARE 306

Query: 121 CGWRSCDSLLASQDPCVVCLERECTVAAQGCDHEFCTSCALYLCSTNCTSSCSHGPPGSI 180
           CGWRSCDSLL  QDPCVVCLERECTVAAQGCDHEFCTSCALYLCSTNCTSS SHGP GSI
Sbjct: 307 CGWRSCDSLLTCQDPCVVCLERECTVAAQGCDHEFCTSCALYLCSTNCTSSVSHGPSGSI 366

Query: 181 ACPLCRNGIVSFVKLPKTKPIAKEIARTSLSLSFCTCSSEVPEPPHTLTTPLCNPEFTCS 240
           ACPLCRNGIVSFVKLP TKPIAKEI RTSLSLSFCTCS E PEPP TLTTPLC PEFTCS
Sbjct: 367 ACPLCRNGIVSFVKLPGTKPIAKEITRTSLSLSFCTCSGEAPEPP-TLTTPLCKPEFTCS 426

Query: 241 QISPPKASCRPLSSQRFPSMILNSNLCMGAPHSSSSSLIPSNVDRNTRNNI--------- 300
           +ISP  AS R LS QRFPSM LNSNLCMG P+SSSSSL+P NVDRN RN++         
Sbjct: 427 RISPLGASFRSLSCQRFPSMKLNSNLCMGDPNSSSSSLVPCNVDRNMRNHVARCSRSSGF 486

Query: 301 -----EGRSSWFSALN-HVMNGCGC 311
                EGR SW SALN +V  G GC
Sbjct: 487 RRTTSEGRRSWLSALNQYVTTGSGC 510

BLAST of Carg08352 vs. ExPASy TrEMBL
Match: A0A1S3BTJ5 (probable E3 ubiquitin-protein ligase XBOS32 OS=Cucumis melo OX=3656 GN=LOC103493384 PE=4 SV=1)

HSP 1 Score: 521.2 bits (1341), Expect = 3.0e-144
Identity = 269/325 (82.77%), Postives = 279/325 (85.85%), Query Frame = 0

Query: 1   MAALNGHVDSVQLLLDLGASASQVTVEDGTTIDLIGAGSTALHYAACGGSAQCCQMLIAR 60
           MAALNGHVDS+QLLLDLGASA QVTVEDGTTIDLIGAGSTALHYAACGG+AQCCQMLIAR
Sbjct: 187 MAALNGHVDSLQLLLDLGASAFQVTVEDGTTIDLIGAGSTALHYAACGGNAQCCQMLIAR 246

Query: 61  GASLTAQNANGSTPLMVARSWHRDWLEEILSKEPEEAATNLVPSPYLTLPLMSIVKIARE 120
           GA LTAQNANG TPLMVARSWHRDWLEEILS+E    ATNLVPSPYLTLPLMSIV+IARE
Sbjct: 247 GADLTAQNANGWTPLMVARSWHRDWLEEILSREAGAVATNLVPSPYLTLPLMSIVRIARE 306

Query: 121 CGWRSCDSLLASQDPCVVCLERECTVAAQGCDHEFCTSCALYLCSTNCTSSCSHGPPGSI 180
           CGWRSCDSLL  QDPCVVCLERECTVAAQGCDHEFCTSCALYLCSTNCTSS SHGP GSI
Sbjct: 307 CGWRSCDSLLTCQDPCVVCLERECTVAAQGCDHEFCTSCALYLCSTNCTSSVSHGPSGSI 366

Query: 181 ACPLCRNGIVSFVKLPKTKPIAKEIARTSLSLSFCTCSSEVPEPPHTLTTPLCNPEFTCS 240
           ACPLCRNGIVSFVKLP TKPIAKEI RTSLSLSFCTCS E PEPP TLTTPLC PEFTCS
Sbjct: 367 ACPLCRNGIVSFVKLPGTKPIAKEITRTSLSLSFCTCSGEAPEPP-TLTTPLCKPEFTCS 426

Query: 241 QISPPKASCRPLSSQRFPSMILNSNLCMGAPHSSSSSLIPSNVDRNTRNNI--------- 300
           +ISP  AS R LS QRFPSM LNSNLCMG P+SSSSSL+P NVDRN RN++         
Sbjct: 427 RISPLGASFRSLSCQRFPSMKLNSNLCMGDPNSSSSSLVPCNVDRNMRNHVARCSRSSGF 486

Query: 301 -----EGRSSWFSALN-HVMNGCGC 311
                EGR SW SALN +V  G GC
Sbjct: 487 RRTTSEGRRSWLSALNQYVTTGSGC 510

BLAST of Carg08352 vs. ExPASy TrEMBL
Match: A0A0A0LK58 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G047800 PE=4 SV=1)

HSP 1 Score: 518.1 bits (1333), Expect = 2.6e-143
Identity = 266/325 (81.85%), Postives = 278/325 (85.54%), Query Frame = 0

Query: 1   MAALNGHVDSVQLLLDLGASASQVTVEDGTTIDLIGAGSTALHYAACGGSAQCCQMLIAR 60
           MAALNGHVDSVQLLLDLGASA QVTVEDGTTIDLIGAGSTALHYAACGG+AQCCQMLIAR
Sbjct: 187 MAALNGHVDSVQLLLDLGASAFQVTVEDGTTIDLIGAGSTALHYAACGGNAQCCQMLIAR 246

Query: 61  GASLTAQNANGSTPLMVARSWHRDWLEEILSKEPEEAATNLVPSPYLTLPLMSIVKIARE 120
           GA LTAQNANG TPLMVARSWHRDWL EILS+E    ATNLVPSPYLTLPLMSIV+IARE
Sbjct: 247 GADLTAQNANGWTPLMVARSWHRDWLVEILSREAGAVATNLVPSPYLTLPLMSIVRIARE 306

Query: 121 CGWRSCDSLLASQDPCVVCLERECTVAAQGCDHEFCTSCALYLCSTNCTSSCSHGPPGSI 180
           CGWRSCDSLL  QDPCVVCLERECTVAAQGCDHEFCT CALYLCSTNCTSS SHGP GSI
Sbjct: 307 CGWRSCDSLLTCQDPCVVCLERECTVAAQGCDHEFCTRCALYLCSTNCTSSVSHGPTGSI 366

Query: 181 ACPLCRNGIVSFVKLPKTKPIAKEIARTSLSLSFCTCSSEVPEPPHTLTTPLCNPEFTCS 240
           ACPLCRNGIVSF+KLP+TKPIAKEI RTSLSLSFCTCS E PEPP TLTTPLC PEFTCS
Sbjct: 367 ACPLCRNGIVSFIKLPRTKPIAKEITRTSLSLSFCTCSGEAPEPP-TLTTPLCKPEFTCS 426

Query: 241 QISPPKASCRPLSSQRFPSMILNSNLCMGAPHSSSSSLIPSNVDRNTRNNI--------- 300
           +ISP  AS R LS QRFPS+ LNSNLCMG PHSSSSSL+P +VDRN RN++         
Sbjct: 427 RISPLGASFRSLSCQRFPSVKLNSNLCMGDPHSSSSSLVPCDVDRNMRNHVSRCSRSSGF 486

Query: 301 -----EGRSSWFSALN-HVMNGCGC 311
                EGR SW SALN +V  G GC
Sbjct: 487 RRTASEGRRSWLSALNQYVTTGSGC 510

BLAST of Carg08352 vs. TAIR 10
Match: AT5G57740.1 (XB3 ortholog 2 in Arabidopsis thaliana )

HSP 1 Score: 331.6 bits (849), Expect = 6.6e-91
Identity = 184/327 (56.27%), Postives = 227/327 (69.42%), Query Frame = 0

Query: 1   MAALNGHVDSVQLLLDLGASASQVTVEDGTTIDLIGAGSTALHYAACGGSAQCCQMLIAR 60
           +AALNGH+++VQLLLDLGAS +QVTVEDGTTIDLIGAGSTALHYA+CGG+ QCCQ+LI++
Sbjct: 184 VAALNGHIETVQLLLDLGASVTQVTVEDGTTIDLIGAGSTALHYASCGGNTQCCQLLISK 243

Query: 61  GASLTAQNANGSTPLMVARSWHRDWLEEILSKEPEEAATNL--VPSPYLTLPLMSIVKIA 120
           GA L A N+NG TP+MVARSWHR+WLEEIL+   E+   +L  VPSP+L LPLMSIV IA
Sbjct: 244 GACLAAVNSNGWTPMMVARSWHRNWLEEILNPTTEQPQLHLPNVPSPFLCLPLMSIVNIA 303

Query: 121 RECGWRSCDSLLASQDPCVVCLERECTVAAQGCDHEFCTSCALYLCSTNCTSS-CSHGPP 180
           +ECGWR  D L   +DPC VCLER+CTVAA GC HEFCT+CALYL +T+ TSS  S+  P
Sbjct: 304 QECGWRENDCLTPCRDPCAVCLERKCTVAADGCAHEFCTNCALYLSTTSITSSKTSNVTP 363

Query: 181 GSIACPLCRNGIVSFVKLPKTKPIAK--EIARTSLSLSFCTCSSEVPEPPHTLTTPLCNP 240
           GS+ CPLCRNGIVSF KLP T    +    +RTS+SLSFCTCSS+V +     T  L NP
Sbjct: 364 GSVPCPLCRNGIVSFTKLPHTTATTRTSTSSRTSISLSFCTCSSDVLD-----TALLTNP 423

Query: 241 EFTCSQI----------SPPKASCRPLSSQRFPSMILNSNLCMGAPHSS--SSSLIPSNV 300
            ++C  +          S   ++ R LS +RFP  +      +  P S     S   S V
Sbjct: 424 HYSCKPVVSRTGSRTPQSARSSAFRSLSCRRFPPSLCLGGSDVDEPRSRLIGGSYSRSGV 483

Query: 301 D-RNTRNNIEGRSSWFSALNHVMNGCG 310
             R + + +EG+ SWFSALNH +   G
Sbjct: 484 GFRRSTSQVEGKRSWFSALNHCVTTGG 505

BLAST of Carg08352 vs. TAIR 10
Match: AT5G07270.1 (XB3 ortholog 3 in Arabidopsis thaliana )

HSP 1 Score: 217.2 bits (552), Expect = 1.8e-56
Identity = 128/277 (46.21%), Postives = 163/277 (58.84%), Query Frame = 0

Query: 1   MAALNGHVDSVQLLLDLGASASQVTVEDGTTIDLIGAGSTALHYAACGGSAQCCQMLIAR 60
           MAALNG  D VQLLLDL A+ S VT   GT++D+IGAGST LHYAACGG+ +CCQ+L+AR
Sbjct: 178 MAALNGLFDCVQLLLDLEANVSAVTFHYGTSMDMIGAGSTPLHYAACGGNLKCCQILLAR 237

Query: 61  GASLTAQNANGSTPLMVARSWHRDWLEEILSKEPEEAATNLVPSPYLTLPLMSIVKIARE 120
           GA     N NG  P+ +AR W R WLE +LS   +        S YL+LPL+SI+ IARE
Sbjct: 238 GARKMTLNCNGWLPIDIARMWSRHWLEPLLSPNSDVVIPAFPHSNYLSLPLLSILNIARE 297

Query: 121 CGWRSCDSLLASQDPCVVCLERECTVAAQGCDHEFCTSCALYLCSTNCTSSCSHGPPGSI 180
            G +S  ++    D C VCLER CTVAA+GC+H+ C  CALYLCS++   S + GPPGSI
Sbjct: 298 FGLQSA-TIGDEVDICAVCLERTCTVAAEGCEHQLCVRCALYLCSSSNVPSVTVGPPGSI 357

Query: 181 ACPLCRNGIVSFVKLPKTKPIAKEIARTSLSLSFC----------TCSSEVPEPPHTLTT 240
            CPLCR+GI +F +LP +  + +E+ +  +SL FC          T  S    PP    +
Sbjct: 358 PCPLCRHGITAFKRLPSS--LTREM-KLPMSLGFCAPCMLHTGDTTDQSSPTCPPTEQRS 417

Query: 241 PLCNPEFTCSQISPPKASCRPLSSQRFPSMILNSNLC 268
                    S I  P  +C P  S   P    N   C
Sbjct: 418 SKTRAASVSSDIFCP-VTCSPFPSVNIPMCTCNEGTC 449

BLAST of Carg08352 vs. TAIR 10
Match: AT2G28840.1 (XB3 ortholog 1 in Arabidopsis thaliana )

HSP 1 Score: 59.3 bits (142), Expect = 6.3e-09
Identity = 61/225 (27.11%), Postives = 89/225 (39.56%), Query Frame = 0

Query: 1   MAALNGHVDSVQLLLDLGASASQVTVEDGTTIDLIGAGSTALHYAACGGSAQCCQMLIAR 60
           +AA     + V +LLD G+     T   G+       GST LH AA  GS  C + L+A 
Sbjct: 164 LAARQRRPECVNVLLDSGSLVCASTSVYGS------PGSTPLHLAARSGSIDCVRKLLAW 223

Query: 61  GASLTAQNANGSTPLMVARSWHRDWLEEILSKEPEEAATNLVPSPYLTL-PLMSIVKIAR 120
           GA    ++A+G  P +VA          +L+  P  A   + PSP   +  L    K+  
Sbjct: 224 GADRLQRDASGRIPYVVAMKHKHGACGALLN--PSSAEPLVWPSPLKFISELNDEAKLLL 283

Query: 121 ECGWRSCD---------------------------SLLASQDPCVVCLERECTVAAQGCD 180
           E      +                           S ++  + C +C E+ CT+  + C 
Sbjct: 284 EQALMEANREREKTILKGTAYSLPSPSFSDTDDNMSEVSDTELCCICFEQVCTIEVKDCG 343

Query: 181 HEFCTSCALYLCSTN----CTSSCSHGPPGSIACPLCRNGIVSFV 194
           H+ C  C L LC  N     TS+ +  PP    CP CR+ I   V
Sbjct: 344 HQMCAQCTLALCCHNKPNPTTSTVT--PP---VCPFCRSTIACLV 375

BLAST of Carg08352 vs. TAIR 10
Match: AT2G28840.2 (XB3 ortholog 1 in Arabidopsis thaliana )

HSP 1 Score: 59.3 bits (142), Expect = 6.3e-09
Identity = 61/225 (27.11%), Postives = 89/225 (39.56%), Query Frame = 0

Query: 1   MAALNGHVDSVQLLLDLGASASQVTVEDGTTIDLIGAGSTALHYAACGGSAQCCQMLIAR 60
           +AA     + V +LLD G+     T   G+       GST LH AA  GS  C + L+A 
Sbjct: 150 LAARQRRPECVNVLLDSGSLVCASTSVYGS------PGSTPLHLAARSGSIDCVRKLLAW 209

Query: 61  GASLTAQNANGSTPLMVARSWHRDWLEEILSKEPEEAATNLVPSPYLTL-PLMSIVKIAR 120
           GA    ++A+G  P +VA          +L+  P  A   + PSP   +  L    K+  
Sbjct: 210 GADRLQRDASGRIPYVVAMKHKHGACGALLN--PSSAEPLVWPSPLKFISELNDEAKLLL 269

Query: 121 ECGWRSCD---------------------------SLLASQDPCVVCLERECTVAAQGCD 180
           E      +                           S ++  + C +C E+ CT+  + C 
Sbjct: 270 EQALMEANREREKTILKGTAYSLPSPSFSDTDDNMSEVSDTELCCICFEQVCTIEVKDCG 329

Query: 181 HEFCTSCALYLCSTN----CTSSCSHGPPGSIACPLCRNGIVSFV 194
           H+ C  C L LC  N     TS+ +  PP    CP CR+ I   V
Sbjct: 330 HQMCAQCTLALCCHNKPNPTTSTVT--PP---VCPFCRSTIACLV 361

BLAST of Carg08352 vs. TAIR 10
Match: AT2G17390.1 (ankyrin repeat-containing 2B )

HSP 1 Score: 45.4 bits (106), Expect = 9.4e-05
Identity = 29/88 (32.95%), Postives = 47/88 (53.41%), Query Frame = 0

Query: 6   GHVDSVQLLLDLGASASQVTVEDGTTIDLIGAGSTALHYAACGGSAQCCQMLIARGASLT 65
           G V   Q+LLD GA+A+ +              +T LHYAA  G  +C  +L+  GA++T
Sbjct: 264 GEVRCAQVLLDAGANANAIDKNK----------NTPLHYAAGYGRKECVSLLLENGAAVT 323

Query: 66  AQNANGSTPLMVARSWHRDWLEEILSKE 94
            QN +   P+ VAR  ++  + ++L K+
Sbjct: 324 QQNMDNKNPIDVARLNNQLDVVKLLEKD 341

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7029787.15.6e-177100.00putative E3 ubiquitin-protein ligase XBOS32, partial [Cucurbita argyrosperma sub... [more]
KAG6598844.12.1e-17699.68E3 ubiquitin-protein ligase XBAT32, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_022929749.14.2e-17297.42E3 ubiquitin-protein ligase XBAT32-like [Cucurbita moschata][more]
XP_023547016.19.3e-17297.42E3 ubiquitin-protein ligase XBAT32-like [Cucurbita pepo subsp. pepo][more]
XP_022997410.11.5e-16996.45E3 ubiquitin-protein ligase XBAT32-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q6NLQ89.3e-9056.27E3 ubiquitin-protein ligase XBAT32 OS=Arabidopsis thaliana OX=3702 GN=XBAT32 PE=... [more]
Q6KAE51.2e-8462.87Probable E3 ubiquitin-protein ligase XBOS32 OS=Oryza sativa subsp. japonica OX=3... [more]
Q7EZ441.0e-7262.10Probable E3 ubiquitin-protein ligase XBOS35 OS=Oryza sativa subsp. japonica OX=3... [more]
Q337A05.1e-5647.97Probable E3 ubiquitin-protein ligase XBOS33 OS=Oryza sativa subsp. japonica OX=3... [more]
Q4FE452.5e-5546.21E3 ubiquitin-protein ligase XBAT33 OS=Arabidopsis thaliana OX=3702 GN=XBAT33 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1EPN82.0e-17297.42E3 ubiquitin-protein ligase XBAT32-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1K9I97.2e-17096.45E3 ubiquitin-protein ligase XBAT32-like OS=Cucurbita maxima OX=3661 GN=LOC111492... [more]
A0A5A7U2Y73.0e-14482.77Putative E3 ubiquitin-protein ligase XBOS32 OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S3BTJ53.0e-14482.77probable E3 ubiquitin-protein ligase XBOS32 OS=Cucumis melo OX=3656 GN=LOC103493... [more]
A0A0A0LK582.6e-14381.85Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G047800 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G57740.16.6e-9156.27XB3 ortholog 2 in Arabidopsis thaliana [more]
AT5G07270.11.8e-5646.21XB3 ortholog 3 in Arabidopsis thaliana [more]
AT2G28840.16.3e-0927.11XB3 ortholog 1 in Arabidopsis thaliana [more]
AT2G28840.26.3e-0927.11XB3 ortholog 1 in Arabidopsis thaliana [more]
AT2G17390.19.4e-0532.95ankyrin repeat-containing 2B [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002110Ankyrin repeatSMARTSM00248ANK_2acoord: 37..66
e-value: 5.5E-4
score: 29.2
IPR002110Ankyrin repeatPROSITEPS50088ANK_REPEATcoord: 37..69
score: 11.35392
IPR036770Ankyrin repeat-containing domain superfamilyGENE3D1.25.40.20coord: 1..106
e-value: 9.4E-20
score: 73.0
IPR036770Ankyrin repeat-containing domain superfamilySUPERFAMILY48403Ankyrin repeatcoord: 2..134
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 124..205
e-value: 1.7E-5
score: 26.0
IPR020683Ankyrin repeat-containing domainPFAMPF12796Ank_2coord: 2..91
e-value: 4.8E-11
score: 43.1
NoneNo IPR availablePANTHERPTHR24128HOMEOBOX PROTEIN WARIAIcoord: 1..305
NoneNo IPR availablePANTHERPTHR24128:SF63E3 UBIQUITIN-PROTEIN LIGASE XBOS32 ISOFORM X1-RELATEDcoord: 1..305
NoneNo IPR availablePROSITEPS50297ANK_REP_REGIONcoord: 37..68
score: 10.152792
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 135..192
IPR017907Zinc finger, RING-type, conserved sitePROSITEPS00518ZF_RING_1coord: 151..160
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 136..186
score: 9.184606

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg08352-RACarg08352-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006464 cellular protein modification process
biological_process GO:0016310 phosphorylation
molecular_function GO:0140096 catalytic activity, acting on a protein
molecular_function GO:0016301 kinase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0005515 protein binding