Carg08313 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg08313 vs. NCBI nr
Match: KAG6598810.1 (E3 ubiquitin-protein ligase MBR2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7029750.1 E3 ubiquitin-protein ligase MBR2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 983.4 bits (2541), Expect = 7.6e-283
Identity = 524/524 (100.00%), Postives = 524/524 (100.00%), Query Frame = 0

Query: 1   MDEYPSKRVANGYTSRRGPSAVMRDTANSRDQDGKYCSRIGCSGRLNSPRSTRSSYSEKL 60
           MDEYPSKRVANGYTSRRGPSAVMRDTANSRDQDGKYCSRIGCSGRLNSPRSTRSSYSEKL
Sbjct: 1   MDEYPSKRVANGYTSRRGPSAVMRDTANSRDQDGKYCSRIGCSGRLNSPRSTRSSYSEKL 60

Query: 61  KSPLQSFRTSSSGKEIAGSSSRTYYAVRGSKKSVAETQRKRPTQLETDSSETSSTQDDSE 120
           KSPLQSFRTSSSGKEIAGSSSRTYYAVRGSKKSVAETQRKRPTQLETDSSETSSTQDDSE
Sbjct: 61  KSPLQSFRTSSSGKEIAGSSSRTYYAVRGSKKSVAETQRKRPTQLETDSSETSSTQDDSE 120

Query: 121 ISQGIPSNERIRKGLHVSLKSSDSTDDTVMEPRSSSVAPNTRGRRDFNQGSGMRNKDTPA 180
           ISQGIPSNERIRKGLHVSLKSSDSTDDTVMEPRSSSVAPNTRGRRDFNQGSGMRNKDTPA
Sbjct: 121 ISQGIPSNERIRKGLHVSLKSSDSTDDTVMEPRSSSVAPNTRGRRDFNQGSGMRNKDTPA 180

Query: 181 SSSVLMGSKSSRPTSRGGADPNLSKLKDTAKKRNSEAESSSTRGKKMNGSSSERRITSSG 240
           SSSVLMGSKSSRPTSRGGADPNLSKLKDTAKKRNSEAESSSTRGKKMNGSSSERRITSSG
Sbjct: 181 SSSVLMGSKSSRPTSRGGADPNLSKLKDTAKKRNSEAESSSTRGKKMNGSSSERRITSSG 240

Query: 241 YGVSISGSRGAKNGTSNRENGIASVRSRTSINGTSRARSQSSRPDRNSTSLHESRSVIYQ 300
           YGVSISGSRGAKNGTSNRENGIASVRSRTSINGTSRARSQSSRPDRNSTSLHESRSVIYQ
Sbjct: 241 YGVSISGSRGAKNGTSNRENGIASVRSRTSINGTSRARSQSSRPDRNSTSLHESRSVIYQ 300

Query: 301 ESQDHQPESSVDNNAHEVSTELISDHPISYRRSDSMNENILSHRPASPAEIGLTRSLTTR 360
           ESQDHQPESSVDNNAHEVSTELISDHPISYRRSDSMNENILSHRPASPAEIGLTRSLTTR
Sbjct: 301 ESQDHQPESSVDNNAHEVSTELISDHPISYRRSDSMNENILSHRPASPAEIGLTRSLTTR 360

Query: 361 DSFRHYVIAEVLLALERIEHEEELTYEQATLLETNLFLSGLNVYDQHRDMRLDIDNMSYE 420
           DSFRHYVIAEVLLALERIEHEEELTYEQATLLETNLFLSGLNVYDQHRDMRLDIDNMSYE
Sbjct: 361 DSFRHYVIAEVLLALERIEHEEELTYEQATLLETNLFLSGLNVYDQHRDMRLDIDNMSYE 420

Query: 421 ELLALEERMGTVSTAVTEEALSQCLNRSIYQSTHAEDTIAGGSEYEDGVKCCICQEEYSN 480
           ELLALEERMGTVSTAVTEEALSQCLNRSIYQSTHAEDTIAGGSEYEDGVKCCICQEEYSN
Sbjct: 421 ELLALEERMGTVSTAVTEEALSQCLNRSIYQSTHAEDTIAGGSEYEDGVKCCICQEEYSN 480

Query: 481 GDEVGMLRCEHTYHEGCIQQWLQLKNWCPICKASVEEPASALPS 525
           GDEVGMLRCEHTYHEGCIQQWLQLKNWCPICKASVEEPASALPS
Sbjct: 481 GDEVGMLRCEHTYHEGCIQQWLQLKNWCPICKASVEEPASALPS 524

BLAST of Carg08313 vs. NCBI nr
Match: XP_023546133.1 (probable E3 ubiquitin-protein ligase RHG1A isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 970.3 bits (2507), Expect = 6.6e-279
Identity = 517/524 (98.66%), Postives = 521/524 (99.43%), Query Frame = 0

Query: 1   MDEYPSKRVANGYTSRRGPSAVMRDTANSRDQDGKYCSRIGCSGRLNSPRSTRSSYSEKL 60
           MDEYPSKRVANGYTSRRGPSAVMRDTANSRDQDGKYCSRIGCSGRLNSP+STRSSYSEKL
Sbjct: 1   MDEYPSKRVANGYTSRRGPSAVMRDTANSRDQDGKYCSRIGCSGRLNSPKSTRSSYSEKL 60

Query: 61  KSPLQSFRTSSSGKEIAGSSSRTYYAVRGSKKSVAETQRKRPTQLETDSSETSSTQDDSE 120
           KSPLQSFRTSSSGKEIAGSSSRTYYAVRGSKKSVAETQRKRPTQLETDSSETSSTQDDSE
Sbjct: 61  KSPLQSFRTSSSGKEIAGSSSRTYYAVRGSKKSVAETQRKRPTQLETDSSETSSTQDDSE 120

Query: 121 ISQGIPSNERIRKGLHVSLKSSDSTDDTVMEPRSSSVAPNTRGRRDFNQGSGMRNKDTPA 180
           ISQGIPSNERIRKGLHVSLKSSDSTDDTVMEPRSSSVAPNTRGRRDFNQGSGMRNKDT A
Sbjct: 121 ISQGIPSNERIRKGLHVSLKSSDSTDDTVMEPRSSSVAPNTRGRRDFNQGSGMRNKDTLA 180

Query: 181 SSSVLMGSKSSRPTSRGGADPNLSKLKDTAKKRNSEAESSSTRGKKMNGSSSERRITSSG 240
           SSSVLMGSKSSRPTSRGGADPNLSKLKDTAKKRNSEAESSSTRGKKMNGSSSERRITSSG
Sbjct: 181 SSSVLMGSKSSRPTSRGGADPNLSKLKDTAKKRNSEAESSSTRGKKMNGSSSERRITSSG 240

Query: 241 YGVSISGSRGAKNGTSNRENGIASVRSRTSINGTSRARSQSSRPDRNSTSLHESRSVIYQ 300
           YGVSISGSR AKNGTSNRENGIASVRSRTSINGTSRARSQS+RPDRNSTSLHESRS+IYQ
Sbjct: 241 YGVSISGSRAAKNGTSNRENGIASVRSRTSINGTSRARSQSNRPDRNSTSLHESRSMIYQ 300

Query: 301 ESQDHQPESSVDNNAHEVSTELISDHPISYRRSDSMNENILSHRPASPAEIGLTRSLTTR 360
           ESQDHQPES+VDNNAHEVSTELISDHPISYRRSDSMNENILSHRPASPAEIGLTRSLTTR
Sbjct: 301 ESQDHQPESAVDNNAHEVSTELISDHPISYRRSDSMNENILSHRPASPAEIGLTRSLTTR 360

Query: 361 DSFRHYVIAEVLLALERIEHEEELTYEQATLLETNLFLSGLNVYDQHRDMRLDIDNMSYE 420
           DSFRHYVIAEVLLALERIEHEEELTYEQATLLETNLFLSGLNVYDQHRDMRLDIDNMSYE
Sbjct: 361 DSFRHYVIAEVLLALERIEHEEELTYEQATLLETNLFLSGLNVYDQHRDMRLDIDNMSYE 420

Query: 421 ELLALEERMGTVSTAVTEEALSQCLNRSIYQSTHAEDTIAGGSEYEDGVKCCICQEEYSN 480
           ELLALEE MGTVSTAVTEEALSQCLNRSIYQSTHAEDTIAGGSEYEDGVKCCICQEEYSN
Sbjct: 421 ELLALEESMGTVSTAVTEEALSQCLNRSIYQSTHAEDTIAGGSEYEDGVKCCICQEEYSN 480

Query: 481 GDEVGMLRCEHTYHEGCIQQWLQLKNWCPICKASVEEPASALPS 525
           GDEVGMLRCEHTYHEGCIQQWLQLKNWCPICKASVEEPASALPS
Sbjct: 481 GDEVGMLRCEHTYHEGCIQQWLQLKNWCPICKASVEEPASALPS 524

BLAST of Carg08313 vs. NCBI nr
Match: XP_022962265.1 (probable E3 ubiquitin-protein ligase RHG1A isoform X2 [Cucurbita moschata])

HSP 1 Score: 966.8 bits (2498), Expect = 7.3e-278
Identity = 516/521 (99.04%), Postives = 518/521 (99.42%), Query Frame = 0

Query: 1   MDEYPSKRVANGYTSRRGPSAVMRDTANSRDQDGKYCSRIGCSGRLNSPRSTRSSYSEKL 60
           MDEYPSKRVANGYTSRRGPSAVMRDTANSRDQDGKYCSRIGCSGRLNSPRSTRSSYSEKL
Sbjct: 1   MDEYPSKRVANGYTSRRGPSAVMRDTANSRDQDGKYCSRIGCSGRLNSPRSTRSSYSEKL 60

Query: 61  KSPLQSFRTSSSGKEIAGSSSRTYYAVRGSKKSVAETQRKRPTQLETDSSETSSTQDDSE 120
           KSPLQSFRTSSSGKEIAGSSSRTYYAVRGSKKSVAETQRKRPTQLETDSSETSSTQDDSE
Sbjct: 61  KSPLQSFRTSSSGKEIAGSSSRTYYAVRGSKKSVAETQRKRPTQLETDSSETSSTQDDSE 120

Query: 121 ISQGIPSNERIRKGLHVSLKSSDSTDDTVMEPRSSSVAPNTRGRRDFNQGSGMRNKDTPA 180
           ISQGIPSNERIRKGLHVSLKSSDSTDDTVMEPRSSSVAPNTRGRRDFNQGSGMRNKDT A
Sbjct: 121 ISQGIPSNERIRKGLHVSLKSSDSTDDTVMEPRSSSVAPNTRGRRDFNQGSGMRNKDTLA 180

Query: 181 SSSVLMGSKSSRPTSRGGADPNLSKLKDTAKKRNSEAESSSTRGKKMNGSSSERRITSSG 240
           SSSVLMGSKSSRPTSRGGADPNLSKLKDTAKKRNSEAESSSTRGKKMNGSSSERRITSSG
Sbjct: 181 SSSVLMGSKSSRPTSRGGADPNLSKLKDTAKKRNSEAESSSTRGKKMNGSSSERRITSSG 240

Query: 241 YGVSISGSRGAKNGTSNRENGIASVRSRTSINGTSRARSQSSRPDRNSTSLHESRSVIYQ 300
           YGVSISGSR AKNGTSNRENGIASVRSRTSINGTSRARSQS+RPDRNSTSLHESRS+IYQ
Sbjct: 241 YGVSISGSRAAKNGTSNRENGIASVRSRTSINGTSRARSQSNRPDRNSTSLHESRSMIYQ 300

Query: 301 ESQDHQPESSVDNNAHEVSTELISDHPISYRRSDSMNENILSHRPASPAEIGLTRSLTTR 360
           ESQDHQPESSVDNNAHEVSTELISDHPISYRRSDSMNENILSHRPASPAEIGLTRSLTTR
Sbjct: 301 ESQDHQPESSVDNNAHEVSTELISDHPISYRRSDSMNENILSHRPASPAEIGLTRSLTTR 360

Query: 361 DSFRHYVIAEVLLALERIEHEEELTYEQATLLETNLFLSGLNVYDQHRDMRLDIDNMSYE 420
           DSFRHYVIAEVLLALERIEHEEELTYEQATLLETNLFLSGLNVYDQHRDMRLDIDNMSYE
Sbjct: 361 DSFRHYVIAEVLLALERIEHEEELTYEQATLLETNLFLSGLNVYDQHRDMRLDIDNMSYE 420

Query: 421 ELLALEERMGTVSTAVTEEALSQCLNRSIYQSTHAEDTIAGGSEYEDGVKCCICQEEYSN 480
           ELLALEERMGTVSTAVTEEALSQCLNRSIYQSTHAEDTIAGGSEYED VKCCICQEEYSN
Sbjct: 421 ELLALEERMGTVSTAVTEEALSQCLNRSIYQSTHAEDTIAGGSEYEDSVKCCICQEEYSN 480

Query: 481 GDEVGMLRCEHTYHEGCIQQWLQLKNWCPICKASVEEPASA 522
           GDEVGMLRCEHTYHEGCIQQWLQLKNWCPICKASVEEPASA
Sbjct: 481 GDEVGMLRCEHTYHEGCIQQWLQLKNWCPICKASVEEPASA 521

BLAST of Carg08313 vs. NCBI nr
Match: XP_023546132.1 (probable E3 ubiquitin-protein ligase RHG1A isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 956.8 bits (2472), Expect = 7.6e-275
Identity = 517/548 (94.34%), Postives = 521/548 (95.07%), Query Frame = 0

Query: 1   MDEYPSKRVANGYTSRRGPSAVMRDTANSRDQDGKYCSRIGCSGRLNSPRSTRSSYSEKL 60
           MDEYPSKRVANGYTSRRGPSAVMRDTANSRDQDGKYCSRIGCSGRLNSP+STRSSYSEKL
Sbjct: 1   MDEYPSKRVANGYTSRRGPSAVMRDTANSRDQDGKYCSRIGCSGRLNSPKSTRSSYSEKL 60

Query: 61  KSPLQSFRTSSSGKEIAGSSSRTYYAVRGSKKSVAETQRKRPTQLETDSSETSSTQDDSE 120
           KSPLQSFRTSSSGKEIAGSSSRTYYAVRGSKKSVAETQRKRPTQLETDSSETSSTQDDSE
Sbjct: 61  KSPLQSFRTSSSGKEIAGSSSRTYYAVRGSKKSVAETQRKRPTQLETDSSETSSTQDDSE 120

Query: 121 ISQGIPSNERIRKGLHVSLKSSDSTDDTVMEPRSSSVAPNTRGRRDFNQGSGMRNKDTPA 180
           ISQGIPSNERIRKGLHVSLKSSDSTDDTVMEPRSSSVAPNTRGRRDFNQGSGMRNKDT A
Sbjct: 121 ISQGIPSNERIRKGLHVSLKSSDSTDDTVMEPRSSSVAPNTRGRRDFNQGSGMRNKDTLA 180

Query: 181 SSSVLMGSKSSRPTSRGGA------------------------DPNLSKLKDTAKKRNSE 240
           SSSVLMGSKSSRPTSRGGA                        DPNLSKLKDTAKKRNSE
Sbjct: 181 SSSVLMGSKSSRPTSRGGAGRHTLRNFRCNSISDVISSGCSSSDPNLSKLKDTAKKRNSE 240

Query: 241 AESSSTRGKKMNGSSSERRITSSGYGVSISGSRGAKNGTSNRENGIASVRSRTSINGTSR 300
           AESSSTRGKKMNGSSSERRITSSGYGVSISGSR AKNGTSNRENGIASVRSRTSINGTSR
Sbjct: 241 AESSSTRGKKMNGSSSERRITSSGYGVSISGSRAAKNGTSNRENGIASVRSRTSINGTSR 300

Query: 301 ARSQSSRPDRNSTSLHESRSVIYQESQDHQPESSVDNNAHEVSTELISDHPISYRRSDSM 360
           ARSQS+RPDRNSTSLHESRS+IYQESQDHQPES+VDNNAHEVSTELISDHPISYRRSDSM
Sbjct: 301 ARSQSNRPDRNSTSLHESRSMIYQESQDHQPESAVDNNAHEVSTELISDHPISYRRSDSM 360

Query: 361 NENILSHRPASPAEIGLTRSLTTRDSFRHYVIAEVLLALERIEHEEELTYEQATLLETNL 420
           NENILSHRPASPAEIGLTRSLTTRDSFRHYVIAEVLLALERIEHEEELTYEQATLLETNL
Sbjct: 361 NENILSHRPASPAEIGLTRSLTTRDSFRHYVIAEVLLALERIEHEEELTYEQATLLETNL 420

Query: 421 FLSGLNVYDQHRDMRLDIDNMSYEELLALEERMGTVSTAVTEEALSQCLNRSIYQSTHAE 480
           FLSGLNVYDQHRDMRLDIDNMSYEELLALEE MGTVSTAVTEEALSQCLNRSIYQSTHAE
Sbjct: 421 FLSGLNVYDQHRDMRLDIDNMSYEELLALEESMGTVSTAVTEEALSQCLNRSIYQSTHAE 480

Query: 481 DTIAGGSEYEDGVKCCICQEEYSNGDEVGMLRCEHTYHEGCIQQWLQLKNWCPICKASVE 525
           DTIAGGSEYEDGVKCCICQEEYSNGDEVGMLRCEHTYHEGCIQQWLQLKNWCPICKASVE
Sbjct: 481 DTIAGGSEYEDGVKCCICQEEYSNGDEVGMLRCEHTYHEGCIQQWLQLKNWCPICKASVE 540

BLAST of Carg08313 vs. NCBI nr
Match: XP_022962264.1 (probable E3 ubiquitin-protein ligase RHG1A isoform X1 [Cucurbita moschata])

HSP 1 Score: 953.4 bits (2463), Expect = 8.4e-274
Identity = 516/545 (94.68%), Postives = 518/545 (95.05%), Query Frame = 0

Query: 1   MDEYPSKRVANGYTSRRGPSAVMRDTANSRDQDGKYCSRIGCSGRLNSPRSTRSSYSEKL 60
           MDEYPSKRVANGYTSRRGPSAVMRDTANSRDQDGKYCSRIGCSGRLNSPRSTRSSYSEKL
Sbjct: 1   MDEYPSKRVANGYTSRRGPSAVMRDTANSRDQDGKYCSRIGCSGRLNSPRSTRSSYSEKL 60

Query: 61  KSPLQSFRTSSSGKEIAGSSSRTYYAVRGSKKSVAETQRKRPTQLETDSSETSSTQDDSE 120
           KSPLQSFRTSSSGKEIAGSSSRTYYAVRGSKKSVAETQRKRPTQLETDSSETSSTQDDSE
Sbjct: 61  KSPLQSFRTSSSGKEIAGSSSRTYYAVRGSKKSVAETQRKRPTQLETDSSETSSTQDDSE 120

Query: 121 ISQGIPSNERIRKGLHVSLKSSDSTDDTVMEPRSSSVAPNTRGRRDFNQGSGMRNKDTPA 180
           ISQGIPSNERIRKGLHVSLKSSDSTDDTVMEPRSSSVAPNTRGRRDFNQGSGMRNKDT A
Sbjct: 121 ISQGIPSNERIRKGLHVSLKSSDSTDDTVMEPRSSSVAPNTRGRRDFNQGSGMRNKDTLA 180

Query: 181 SSSVLMGSKSSRPTSRGGA------------------------DPNLSKLKDTAKKRNSE 240
           SSSVLMGSKSSRPTSRGGA                        DPNLSKLKDTAKKRNSE
Sbjct: 181 SSSVLMGSKSSRPTSRGGAGRHTLRNFRCNSISDVISSGCSSSDPNLSKLKDTAKKRNSE 240

Query: 241 AESSSTRGKKMNGSSSERRITSSGYGVSISGSRGAKNGTSNRENGIASVRSRTSINGTSR 300
           AESSSTRGKKMNGSSSERRITSSGYGVSISGSR AKNGTSNRENGIASVRSRTSINGTSR
Sbjct: 241 AESSSTRGKKMNGSSSERRITSSGYGVSISGSRAAKNGTSNRENGIASVRSRTSINGTSR 300

Query: 301 ARSQSSRPDRNSTSLHESRSVIYQESQDHQPESSVDNNAHEVSTELISDHPISYRRSDSM 360
           ARSQS+RPDRNSTSLHESRS+IYQESQDHQPESSVDNNAHEVSTELISDHPISYRRSDSM
Sbjct: 301 ARSQSNRPDRNSTSLHESRSMIYQESQDHQPESSVDNNAHEVSTELISDHPISYRRSDSM 360

Query: 361 NENILSHRPASPAEIGLTRSLTTRDSFRHYVIAEVLLALERIEHEEELTYEQATLLETNL 420
           NENILSHRPASPAEIGLTRSLTTRDSFRHYVIAEVLLALERIEHEEELTYEQATLLETNL
Sbjct: 361 NENILSHRPASPAEIGLTRSLTTRDSFRHYVIAEVLLALERIEHEEELTYEQATLLETNL 420

Query: 421 FLSGLNVYDQHRDMRLDIDNMSYEELLALEERMGTVSTAVTEEALSQCLNRSIYQSTHAE 480
           FLSGLNVYDQHRDMRLDIDNMSYEELLALEERMGTVSTAVTEEALSQCLNRSIYQSTHAE
Sbjct: 421 FLSGLNVYDQHRDMRLDIDNMSYEELLALEERMGTVSTAVTEEALSQCLNRSIYQSTHAE 480

Query: 481 DTIAGGSEYEDGVKCCICQEEYSNGDEVGMLRCEHTYHEGCIQQWLQLKNWCPICKASVE 522
           DTIAGGSEYED VKCCICQEEYSNGDEVGMLRCEHTYHEGCIQQWLQLKNWCPICKASVE
Sbjct: 481 DTIAGGSEYEDSVKCCICQEEYSNGDEVGMLRCEHTYHEGCIQQWLQLKNWCPICKASVE 540

BLAST of Carg08313 vs. ExPASy Swiss-Prot
Match: O49500 (E3 ubiquitin-protein ligase MBR2 OS=Arabidopsis thaliana OX=3702 GN=MBR2 PE=1 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 1.1e-31
Identity = 69/160 (43.12%), Postives = 101/160 (63.12%), Query Frame = 0

Query: 353 LTRSLTTRDSFRHYVIAEVLLALERIEHEEELTYEQATLLETNLFLSGLNVYDQHRDMRL 412
           L RSL   +  R+ +I+E+   L  +   E L +E   + +  ++     ++D+HRDMRL
Sbjct: 508 LGRSLAADNDGRNRLISEIRQVLSAMRRGENLRFEDYMVFDPLIYQGMAEMHDRHRDMRL 567

Query: 413 DIDNMSYEELLALEERMGTVSTAVTEEALSQCLNRSIYQSTHAEDTIAGGSEYEDGVKCC 472
           D+DNMSYEELLAL ER+G VST ++EE + + + +      H   + A GS ++D   CC
Sbjct: 568 DVDNMSYEELLALGERIGDVSTGLSEEVILKVMKQ------HKHTSSAAGS-HQDMEPCC 627

Query: 473 ICQEEYSNGDEVGMLRCEHTYHEGCIQQWLQLKNWCPICK 513
           +CQEEY+ GD++G L C H +H  C++QWL LKN CPICK
Sbjct: 628 VCQEEYAEGDDLGTLGCGHEFHTACVKQWLMLKNLCPICK 660

BLAST of Carg08313 vs. ExPASy Swiss-Prot
Match: Q7XTV7 (Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica OX=39947 GN=HIP1 PE=1 SV=2)

HSP 1 Score: 139.0 bits (349), Expect = 1.5e-31
Identity = 72/161 (44.72%), Postives = 104/161 (64.60%), Query Frame = 0

Query: 352 GLTRSLTTRDSFRHYVIAEVLLALERIEHEEELTYEQATLLETNLFLSGLNVYDQHRDMR 411
           G+  S  +R+  R   + E+  ALE I   E + +E       ++F  G++++D+HRDMR
Sbjct: 518 GVPLSTRSREGRR---LIEIRNALEMIHRGENVRFE-------SIFYGGVDIHDRHRDMR 577

Query: 412 LDIDNMSYEELLALEERMGTVSTAVTEEALSQCLNRSIYQSTHAEDTIAGGSEYEDGVKC 471
           LDIDNMSYEELLALEER+G VST ++EE +++ L +  + S   E ++       +   C
Sbjct: 578 LDIDNMSYEELLALEERIGNVSTGLSEEEVTKLLKQRKFSSWRLEASV-------EEEPC 637

Query: 472 CICQEEYSNGDEVGMLRCEHTYHEGCIQQWLQLKNWCPICK 513
           CICQEEY +GD++G L C H +H GC++QWL +KN CPICK
Sbjct: 638 CICQEEYVDGDDLGTLDCGHDFHVGCVRQWLVVKNTCPICK 661

BLAST of Carg08313 vs. ExPASy Swiss-Prot
Match: Q9ZQF9 (E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana OX=3702 GN=MBR1 PE=1 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 1.4e-29
Identity = 64/158 (40.51%), Postives = 98/158 (62.03%), Query Frame = 0

Query: 355 RSLTTRDSFRHYVIAEVLLALERIEHEEELTYEQATLLETNLFLSGLNVYDQHRDMRLDI 414
           RSL    + R+ +I+E+   L  +   E L  E   + +  ++    +++D+HR+MRLD+
Sbjct: 544 RSLAADGNGRNQIISEIRQVLHAMRRGENLRVEDYMVFDPLIYQGMTDMHDRHREMRLDV 603

Query: 415 DNMSYEELLALEERMGTVSTAVTEEALSQCLNRSIYQSTHAEDTIAGGSEYEDGVKCCIC 474
           DNMSYEELLAL ER+G VST ++EE + + +     Q  H   + +    +++   CCIC
Sbjct: 604 DNMSYEELLALGERIGDVSTGLSEEVILKAMK----QHKHTSSSPSSVELHQNIEPCCIC 663

Query: 475 QEEYSNGDEVGMLRCEHTYHEGCIQQWLQLKNWCPICK 513
           QEEY  GD +G L+C H +H+ CI+QW+ +KN CPICK
Sbjct: 664 QEEYVEGDNLGTLKCGHEFHKDCIKQWVMIKNLCPICK 697

BLAST of Carg08313 vs. ExPASy Swiss-Prot
Match: Q5QLR5 (Probable E3 ubiquitin-protein ligase ZFP1 OS=Oryza sativa subsp. japonica OX=39947 GN=ZFP1 PE=1 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 2.3e-24
Identity = 56/128 (43.75%), Postives = 80/128 (62.50%), Query Frame = 0

Query: 387 EQATLLETNLFLSGLNVYDQHRDMRLDIDNMSYEELLALEERMGTVSTAVTEEALSQCLN 446
           E AT+++ +      NV D+HRDMRLDID+M+YEELLALEE++G V+T + +  + + L 
Sbjct: 387 EHATIMDFSRLYEVSNVVDEHRDMRLDIDSMTYEELLALEEQIGDVNTGLAKSYIVEKLK 446

Query: 447 RSIYQSTHAEDTIAGGSEYEDGVKCCICQEEYSNGDEVGMLRCEHTYHEGCIQQWLQLKN 506
            S++    +  +        +   C ICQEEY   + +G L C H YHE CI+QWL +KN
Sbjct: 447 TSLFVPGSSCMSNKSSESSMENDACIICQEEYQVKECIGTLDCGHRYHEDCIKQWLMVKN 506

Query: 507 WCPICKAS 515
            CPICK +
Sbjct: 507 LCPICKTT 514

BLAST of Carg08313 vs. ExPASy Swiss-Prot
Match: Q9FMM4 (Probable E3 ubiquitin-protein ligase RHG1A OS=Arabidopsis thaliana OX=3702 GN=RHG1A PE=2 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 6.3e-22
Identity = 102/367 (27.79%), Postives = 160/367 (43.60%), Query Frame = 0

Query: 183 SVLMGSKSSRPTSRGGADPNLSKLKDTAKKRNSEAESSSTRGKKMNGSSSERRITSS--- 242
           S+  GS++  P  R      +    ++            TR + + G++ E  + ++   
Sbjct: 329 SIPPGSRTMLPPFRWTGSSLVGGTSNSTAPVERNLHLDETRSRSIPGNTLEIPMFAAPEV 388

Query: 243 GYGVSISGSRGAKNGTSNRENGIASVRSRTSINGT------------------SRARSQS 302
           G       SR   NG  N  + ++   S TS+                      R R++ 
Sbjct: 389 GNFARSQSSRNVTNGNLNSASSVSRTGSTTSVPPPPPPSSNLAWTSYQNSPHYQRRRTER 448

Query: 303 SRPDRNSTSLHESRSVIYQESQDH------QPESSVDNNAHEVSTELISDHPISYRRS-- 362
           S   R S     +     Q S DH       P +S D    +   +    H  +Y R+  
Sbjct: 449 SELARRSLLSSLAADATNQRSGDHPTLRSLAPPASSDGLVLQPGGDNSQMHNRAYSRAGP 508

Query: 363 --DSMNENILS----HRPASPAEIGLTRSLTTRDSFRHYVIAEVLLALERIEHEEELTYE 422
             D   ++++      R  + A  G +R + ++       +  VL  + R  +   L  E
Sbjct: 509 LFDRQGDSVVGIPHPLRALAAASRGRSRLMVSQ-------MQNVLDVMRRDANNNNLRLE 568

Query: 423 QATLLETNLFLSGLNVYDQHRDMRLDIDNMSYEELLALEERMGTVSTAVTEEALSQCLNR 482
              LL  ++   G   +D++RDMRLD+DNMSYEELLALEER+G V T V EE +S  L +
Sbjct: 569 DVMLLNHSVLFDGATGHDRYRDMRLDVDNMSYEELLALEERIGDVCTGVNEETISNRLKQ 628

Query: 483 SIYQSTHAEDTIAGGSEYEDGVKCCICQEEYSNGDEVGMLRCEHTYHEGCIQQWLQLKNW 515
             Y+S             +D   CC+CQEEY+ G+++G L C H +H  CI++WL+ KN 
Sbjct: 629 RKYKSNTKSP--------QDAEPCCVCQEEYTEGEDMGTLECGHEFHSQCIKEWLKQKNL 680

BLAST of Carg08313 vs. ExPASy TrEMBL
Match: A0A6J1HEJ8 (probable E3 ubiquitin-protein ligase RHG1A isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111462767 PE=4 SV=1)

HSP 1 Score: 966.8 bits (2498), Expect = 3.5e-278
Identity = 516/521 (99.04%), Postives = 518/521 (99.42%), Query Frame = 0

Query: 1   MDEYPSKRVANGYTSRRGPSAVMRDTANSRDQDGKYCSRIGCSGRLNSPRSTRSSYSEKL 60
           MDEYPSKRVANGYTSRRGPSAVMRDTANSRDQDGKYCSRIGCSGRLNSPRSTRSSYSEKL
Sbjct: 1   MDEYPSKRVANGYTSRRGPSAVMRDTANSRDQDGKYCSRIGCSGRLNSPRSTRSSYSEKL 60

Query: 61  KSPLQSFRTSSSGKEIAGSSSRTYYAVRGSKKSVAETQRKRPTQLETDSSETSSTQDDSE 120
           KSPLQSFRTSSSGKEIAGSSSRTYYAVRGSKKSVAETQRKRPTQLETDSSETSSTQDDSE
Sbjct: 61  KSPLQSFRTSSSGKEIAGSSSRTYYAVRGSKKSVAETQRKRPTQLETDSSETSSTQDDSE 120

Query: 121 ISQGIPSNERIRKGLHVSLKSSDSTDDTVMEPRSSSVAPNTRGRRDFNQGSGMRNKDTPA 180
           ISQGIPSNERIRKGLHVSLKSSDSTDDTVMEPRSSSVAPNTRGRRDFNQGSGMRNKDT A
Sbjct: 121 ISQGIPSNERIRKGLHVSLKSSDSTDDTVMEPRSSSVAPNTRGRRDFNQGSGMRNKDTLA 180

Query: 181 SSSVLMGSKSSRPTSRGGADPNLSKLKDTAKKRNSEAESSSTRGKKMNGSSSERRITSSG 240
           SSSVLMGSKSSRPTSRGGADPNLSKLKDTAKKRNSEAESSSTRGKKMNGSSSERRITSSG
Sbjct: 181 SSSVLMGSKSSRPTSRGGADPNLSKLKDTAKKRNSEAESSSTRGKKMNGSSSERRITSSG 240

Query: 241 YGVSISGSRGAKNGTSNRENGIASVRSRTSINGTSRARSQSSRPDRNSTSLHESRSVIYQ 300
           YGVSISGSR AKNGTSNRENGIASVRSRTSINGTSRARSQS+RPDRNSTSLHESRS+IYQ
Sbjct: 241 YGVSISGSRAAKNGTSNRENGIASVRSRTSINGTSRARSQSNRPDRNSTSLHESRSMIYQ 300

Query: 301 ESQDHQPESSVDNNAHEVSTELISDHPISYRRSDSMNENILSHRPASPAEIGLTRSLTTR 360
           ESQDHQPESSVDNNAHEVSTELISDHPISYRRSDSMNENILSHRPASPAEIGLTRSLTTR
Sbjct: 301 ESQDHQPESSVDNNAHEVSTELISDHPISYRRSDSMNENILSHRPASPAEIGLTRSLTTR 360

Query: 361 DSFRHYVIAEVLLALERIEHEEELTYEQATLLETNLFLSGLNVYDQHRDMRLDIDNMSYE 420
           DSFRHYVIAEVLLALERIEHEEELTYEQATLLETNLFLSGLNVYDQHRDMRLDIDNMSYE
Sbjct: 361 DSFRHYVIAEVLLALERIEHEEELTYEQATLLETNLFLSGLNVYDQHRDMRLDIDNMSYE 420

Query: 421 ELLALEERMGTVSTAVTEEALSQCLNRSIYQSTHAEDTIAGGSEYEDGVKCCICQEEYSN 480
           ELLALEERMGTVSTAVTEEALSQCLNRSIYQSTHAEDTIAGGSEYED VKCCICQEEYSN
Sbjct: 421 ELLALEERMGTVSTAVTEEALSQCLNRSIYQSTHAEDTIAGGSEYEDSVKCCICQEEYSN 480

Query: 481 GDEVGMLRCEHTYHEGCIQQWLQLKNWCPICKASVEEPASA 522
           GDEVGMLRCEHTYHEGCIQQWLQLKNWCPICKASVEEPASA
Sbjct: 481 GDEVGMLRCEHTYHEGCIQQWLQLKNWCPICKASVEEPASA 521

BLAST of Carg08313 vs. ExPASy TrEMBL
Match: A0A6J1HGK5 (probable E3 ubiquitin-protein ligase RHG1A isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111462767 PE=4 SV=1)

HSP 1 Score: 953.4 bits (2463), Expect = 4.1e-274
Identity = 516/545 (94.68%), Postives = 518/545 (95.05%), Query Frame = 0

Query: 1   MDEYPSKRVANGYTSRRGPSAVMRDTANSRDQDGKYCSRIGCSGRLNSPRSTRSSYSEKL 60
           MDEYPSKRVANGYTSRRGPSAVMRDTANSRDQDGKYCSRIGCSGRLNSPRSTRSSYSEKL
Sbjct: 1   MDEYPSKRVANGYTSRRGPSAVMRDTANSRDQDGKYCSRIGCSGRLNSPRSTRSSYSEKL 60

Query: 61  KSPLQSFRTSSSGKEIAGSSSRTYYAVRGSKKSVAETQRKRPTQLETDSSETSSTQDDSE 120
           KSPLQSFRTSSSGKEIAGSSSRTYYAVRGSKKSVAETQRKRPTQLETDSSETSSTQDDSE
Sbjct: 61  KSPLQSFRTSSSGKEIAGSSSRTYYAVRGSKKSVAETQRKRPTQLETDSSETSSTQDDSE 120

Query: 121 ISQGIPSNERIRKGLHVSLKSSDSTDDTVMEPRSSSVAPNTRGRRDFNQGSGMRNKDTPA 180
           ISQGIPSNERIRKGLHVSLKSSDSTDDTVMEPRSSSVAPNTRGRRDFNQGSGMRNKDT A
Sbjct: 121 ISQGIPSNERIRKGLHVSLKSSDSTDDTVMEPRSSSVAPNTRGRRDFNQGSGMRNKDTLA 180

Query: 181 SSSVLMGSKSSRPTSRGGA------------------------DPNLSKLKDTAKKRNSE 240
           SSSVLMGSKSSRPTSRGGA                        DPNLSKLKDTAKKRNSE
Sbjct: 181 SSSVLMGSKSSRPTSRGGAGRHTLRNFRCNSISDVISSGCSSSDPNLSKLKDTAKKRNSE 240

Query: 241 AESSSTRGKKMNGSSSERRITSSGYGVSISGSRGAKNGTSNRENGIASVRSRTSINGTSR 300
           AESSSTRGKKMNGSSSERRITSSGYGVSISGSR AKNGTSNRENGIASVRSRTSINGTSR
Sbjct: 241 AESSSTRGKKMNGSSSERRITSSGYGVSISGSRAAKNGTSNRENGIASVRSRTSINGTSR 300

Query: 301 ARSQSSRPDRNSTSLHESRSVIYQESQDHQPESSVDNNAHEVSTELISDHPISYRRSDSM 360
           ARSQS+RPDRNSTSLHESRS+IYQESQDHQPESSVDNNAHEVSTELISDHPISYRRSDSM
Sbjct: 301 ARSQSNRPDRNSTSLHESRSMIYQESQDHQPESSVDNNAHEVSTELISDHPISYRRSDSM 360

Query: 361 NENILSHRPASPAEIGLTRSLTTRDSFRHYVIAEVLLALERIEHEEELTYEQATLLETNL 420
           NENILSHRPASPAEIGLTRSLTTRDSFRHYVIAEVLLALERIEHEEELTYEQATLLETNL
Sbjct: 361 NENILSHRPASPAEIGLTRSLTTRDSFRHYVIAEVLLALERIEHEEELTYEQATLLETNL 420

Query: 421 FLSGLNVYDQHRDMRLDIDNMSYEELLALEERMGTVSTAVTEEALSQCLNRSIYQSTHAE 480
           FLSGLNVYDQHRDMRLDIDNMSYEELLALEERMGTVSTAVTEEALSQCLNRSIYQSTHAE
Sbjct: 421 FLSGLNVYDQHRDMRLDIDNMSYEELLALEERMGTVSTAVTEEALSQCLNRSIYQSTHAE 480

Query: 481 DTIAGGSEYEDGVKCCICQEEYSNGDEVGMLRCEHTYHEGCIQQWLQLKNWCPICKASVE 522
           DTIAGGSEYED VKCCICQEEYSNGDEVGMLRCEHTYHEGCIQQWLQLKNWCPICKASVE
Sbjct: 481 DTIAGGSEYEDSVKCCICQEEYSNGDEVGMLRCEHTYHEGCIQQWLQLKNWCPICKASVE 540

BLAST of Carg08313 vs. ExPASy TrEMBL
Match: A0A6J1KB95 (probable E3 ubiquitin-protein ligase RHG1A isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111492329 PE=4 SV=1)

HSP 1 Score: 950.3 bits (2455), Expect = 3.4e-273
Identity = 509/524 (97.14%), Postives = 515/524 (98.28%), Query Frame = 0

Query: 1   MDEYPSKRVANGYTSRRGPSAVMRDTANSRDQDGKYCSRIGCSGRLNSPRSTRSSYSEKL 60
           MDEYPSKRVANGYTSRRGPSAVMRDTANSRDQDGKYCSRIGCSGRLNSP+STRSSYSEKL
Sbjct: 1   MDEYPSKRVANGYTSRRGPSAVMRDTANSRDQDGKYCSRIGCSGRLNSPKSTRSSYSEKL 60

Query: 61  KSPLQSFRTSSSGKEIAGSSSRTYYAVRGSKKSVAETQRKRPTQLETDSSETSSTQDDSE 120
           KSPLQSFRTSSSGKEIAGSSSRTYYAVRGSKKSVAETQ KRPTQLETDSSETSSTQDDSE
Sbjct: 61  KSPLQSFRTSSSGKEIAGSSSRTYYAVRGSKKSVAETQTKRPTQLETDSSETSSTQDDSE 120

Query: 121 ISQGIPSNERIRKGLHVSLKSSDSTDDTVMEPRSSSVAPNTRGRRDFNQGSGMRNKDTPA 180
           ISQGIPSNERIRKGLHVSLKSS+STD TVMEP+SS VAPNTRGRRDFNQGSGMRNKDT A
Sbjct: 121 ISQGIPSNERIRKGLHVSLKSSNSTDHTVMEPQSSIVAPNTRGRRDFNQGSGMRNKDTLA 180

Query: 181 SSSVLMGSKSSRPTSRGGADPNLSKLKDTAKKRNSEAESSSTRGKKMNGSSSERRITSSG 240
           SSSVLMGSKSSRP SRGGADPNLSKLKDTAKKRNSEAESSSTRGKK+NGSSSERRITSSG
Sbjct: 181 SSSVLMGSKSSRPASRGGADPNLSKLKDTAKKRNSEAESSSTRGKKINGSSSERRITSSG 240

Query: 241 YGVSISGSRGAKNGTSNRENGIASVRSRTSINGTSRARSQSSRPDRNSTSLHESRSVIYQ 300
           YGVSISGSRGAKNGTSNRENGIA VRSRTSINGTSRARSQS+RPDRNSTSLHESRSVIYQ
Sbjct: 241 YGVSISGSRGAKNGTSNRENGIALVRSRTSINGTSRARSQSNRPDRNSTSLHESRSVIYQ 300

Query: 301 ESQDHQPESSVDNNAHEVSTELISDHPISYRRSDSMNENILSHRPASPAEIGLTRSLTTR 360
           ESQDHQPESSVDNNA EVSTELISDHPISYRRSDSMNENILSHR ASPAEIGLTRSLTTR
Sbjct: 301 ESQDHQPESSVDNNAREVSTELISDHPISYRRSDSMNENILSHRSASPAEIGLTRSLTTR 360

Query: 361 DSFRHYVIAEVLLALERIEHEEELTYEQATLLETNLFLSGLNVYDQHRDMRLDIDNMSYE 420
           DSFRHYVIAEVLLALERIEHEEELTYEQATLLETNLFLSGLNVYDQHRDMRLDIDNMSYE
Sbjct: 361 DSFRHYVIAEVLLALERIEHEEELTYEQATLLETNLFLSGLNVYDQHRDMRLDIDNMSYE 420

Query: 421 ELLALEERMGTVSTAVTEEALSQCLNRSIYQSTHAEDTIAGGSEYEDGVKCCICQEEYSN 480
           ELLALEERMGTVSTAVTEEALSQCLNRSIYQSTHAEDTI+GGSEYEDGVKCCICQEEYSN
Sbjct: 421 ELLALEERMGTVSTAVTEEALSQCLNRSIYQSTHAEDTISGGSEYEDGVKCCICQEEYSN 480

Query: 481 GDEVGMLRCEHTYHEGCIQQWLQLKNWCPICKASVEEPASALPS 525
           GDEVGMLRCEHTYHEGCIQQWLQLKNWCPICKASVEE ASALPS
Sbjct: 481 GDEVGMLRCEHTYHEGCIQQWLQLKNWCPICKASVEESASALPS 524

BLAST of Carg08313 vs. ExPASy TrEMBL
Match: A0A6J1KDR4 (probable E3 ubiquitin-protein ligase RHG1A isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111492329 PE=4 SV=1)

HSP 1 Score: 936.8 bits (2420), Expect = 3.9e-269
Identity = 509/548 (92.88%), Postives = 515/548 (93.98%), Query Frame = 0

Query: 1   MDEYPSKRVANGYTSRRGPSAVMRDTANSRDQDGKYCSRIGCSGRLNSPRSTRSSYSEKL 60
           MDEYPSKRVANGYTSRRGPSAVMRDTANSRDQDGKYCSRIGCSGRLNSP+STRSSYSEKL
Sbjct: 1   MDEYPSKRVANGYTSRRGPSAVMRDTANSRDQDGKYCSRIGCSGRLNSPKSTRSSYSEKL 60

Query: 61  KSPLQSFRTSSSGKEIAGSSSRTYYAVRGSKKSVAETQRKRPTQLETDSSETSSTQDDSE 120
           KSPLQSFRTSSSGKEIAGSSSRTYYAVRGSKKSVAETQ KRPTQLETDSSETSSTQDDSE
Sbjct: 61  KSPLQSFRTSSSGKEIAGSSSRTYYAVRGSKKSVAETQTKRPTQLETDSSETSSTQDDSE 120

Query: 121 ISQGIPSNERIRKGLHVSLKSSDSTDDTVMEPRSSSVAPNTRGRRDFNQGSGMRNKDTPA 180
           ISQGIPSNERIRKGLHVSLKSS+STD TVMEP+SS VAPNTRGRRDFNQGSGMRNKDT A
Sbjct: 121 ISQGIPSNERIRKGLHVSLKSSNSTDHTVMEPQSSIVAPNTRGRRDFNQGSGMRNKDTLA 180

Query: 181 SSSVLMGSKSSRPTSRGGA------------------------DPNLSKLKDTAKKRNSE 240
           SSSVLMGSKSSRP SRGGA                        DPNLSKLKDTAKKRNSE
Sbjct: 181 SSSVLMGSKSSRPASRGGAGRHTLRNFRCNSISDVISSGCSSSDPNLSKLKDTAKKRNSE 240

Query: 241 AESSSTRGKKMNGSSSERRITSSGYGVSISGSRGAKNGTSNRENGIASVRSRTSINGTSR 300
           AESSSTRGKK+NGSSSERRITSSGYGVSISGSRGAKNGTSNRENGIA VRSRTSINGTSR
Sbjct: 241 AESSSTRGKKINGSSSERRITSSGYGVSISGSRGAKNGTSNRENGIALVRSRTSINGTSR 300

Query: 301 ARSQSSRPDRNSTSLHESRSVIYQESQDHQPESSVDNNAHEVSTELISDHPISYRRSDSM 360
           ARSQS+RPDRNSTSLHESRSVIYQESQDHQPESSVDNNA EVSTELISDHPISYRRSDSM
Sbjct: 301 ARSQSNRPDRNSTSLHESRSVIYQESQDHQPESSVDNNAREVSTELISDHPISYRRSDSM 360

Query: 361 NENILSHRPASPAEIGLTRSLTTRDSFRHYVIAEVLLALERIEHEEELTYEQATLLETNL 420
           NENILSHR ASPAEIGLTRSLTTRDSFRHYVIAEVLLALERIEHEEELTYEQATLLETNL
Sbjct: 361 NENILSHRSASPAEIGLTRSLTTRDSFRHYVIAEVLLALERIEHEEELTYEQATLLETNL 420

Query: 421 FLSGLNVYDQHRDMRLDIDNMSYEELLALEERMGTVSTAVTEEALSQCLNRSIYQSTHAE 480
           FLSGLNVYDQHRDMRLDIDNMSYEELLALEERMGTVSTAVTEEALSQCLNRSIYQSTHAE
Sbjct: 421 FLSGLNVYDQHRDMRLDIDNMSYEELLALEERMGTVSTAVTEEALSQCLNRSIYQSTHAE 480

Query: 481 DTIAGGSEYEDGVKCCICQEEYSNGDEVGMLRCEHTYHEGCIQQWLQLKNWCPICKASVE 525
           DTI+GGSEYEDGVKCCICQEEYSNGDEVGMLRCEHTYHEGCIQQWLQLKNWCPICKASVE
Sbjct: 481 DTISGGSEYEDGVKCCICQEEYSNGDEVGMLRCEHTYHEGCIQQWLQLKNWCPICKASVE 540

BLAST of Carg08313 vs. ExPASy TrEMBL
Match: A0A1S3BCL3 (dentin sialophosphoprotein-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103488230 PE=4 SV=1)

HSP 1 Score: 855.1 bits (2208), Expect = 1.5e-244
Identity = 459/524 (87.60%), Postives = 480/524 (91.60%), Query Frame = 0

Query: 1   MDEYPSKRVANGYTSRRGPSAVMRDTANSRDQDGKYCSRIGCSGRLNSPRSTRSSYSEKL 60
           MD+YPSKRVA+GYTSRRGPSA++RDTANSRDQDGKYCSRIGCSGRLNSP+STR SYSEK 
Sbjct: 1   MDDYPSKRVADGYTSRRGPSAILRDTANSRDQDGKYCSRIGCSGRLNSPKSTRVSYSEKH 60

Query: 61  KSPLQSFRTSSSGKEIAGSSSRTYYAVRGSKKSVAETQRKRPTQLETDSSETSSTQDDSE 120
           KSPLQSFRTSSSGKE AGSSS+TYYAVRGSKKSVAETQRK  TQLETDSSETSSTQDD E
Sbjct: 61  KSPLQSFRTSSSGKETAGSSSKTYYAVRGSKKSVAETQRKLSTQLETDSSETSSTQDDPE 120

Query: 121 ISQGIPSNERIRKGLHVSLKSSDSTDDTVMEPRSSSVAPNTRGRRDFNQGSGMRNKDTPA 180
           ISQ IPS+ERIR GL V LKSS+STDDT+MEP SSSVAPNTRGRRDFNQ SG RNKDTP 
Sbjct: 121 ISQVIPSDERIRTGLRVGLKSSNSTDDTIMEPGSSSVAPNTRGRRDFNQRSGTRNKDTPE 180

Query: 181 SSSVLMGSKSSRPTSRGGADPNLSKLKDTAKKRNSEAESSSTRGKKMNGSSSERRITSSG 240
           +SSVL  SKS+RPT+RGGADPN SK KDTAKKRN EAESSST+GKKMNGSS ERR+TSSG
Sbjct: 181 TSSVLTDSKSTRPTARGGADPNSSKRKDTAKKRNPEAESSSTKGKKMNGSSLERRVTSSG 240

Query: 241 YGVSISGSRGAKNGTSNRENGIASVRSRTSINGTSRARSQSSRPDRNSTSLHESRSVIYQ 300
           YGVSI+ SRGAK GTSNRENGI S RSRT  NGTSRARS  +RPDRNSTSLHESRS+I Q
Sbjct: 241 YGVSIADSRGAKKGTSNRENGITSGRSRTLNNGTSRARSHGNRPDRNSTSLHESRSMISQ 300

Query: 301 ESQDHQPESSVDNNAHEVSTELISDHPISYRRSDSMNENILSHRPASPAEIGLTRSLTTR 360
            SQDHQP+SSVDN  HEVSTELI DHPISYRRSDSMNENILSHRPASPA+IGL RSLTTR
Sbjct: 301 VSQDHQPDSSVDNITHEVSTELIPDHPISYRRSDSMNENILSHRPASPADIGLARSLTTR 360

Query: 361 DSFRHYVIAEVLLALERIEHEEELTYEQATLLETNLFLSGLNVYDQHRDMRLDIDNMSYE 420
           DSFRHY IAEVLLALERIEHEEELTYEQ  LLETNLFLSGLN YDQHRDMRLDIDNMSYE
Sbjct: 361 DSFRHYGIAEVLLALERIEHEEELTYEQVILLETNLFLSGLNFYDQHRDMRLDIDNMSYE 420

Query: 421 ELLALEERMGTVSTAVTEEALSQCLNRSIYQSTHAEDTIAGGSEYEDGVKCCICQEEYSN 480
           ELLALEERMGTVSTAVTEEALSQCLNRS YQS HAEDT AGGSEYEDGVKCCICQEEY N
Sbjct: 421 ELLALEERMGTVSTAVTEEALSQCLNRSTYQSRHAEDTSAGGSEYEDGVKCCICQEEYLN 480

Query: 481 GDEVGMLRCEHTYHEGCIQQWLQLKNWCPICKASVEEPASALPS 525
           GDEVG L+CEHTYHEGCI QWL+LKNWCPICKASVEEPASALPS
Sbjct: 481 GDEVGTLQCEHTYHEGCIHQWLRLKNWCPICKASVEEPASALPS 524

BLAST of Carg08313 vs. TAIR 10
Match: AT5G24870.1 (RING/U-box superfamily protein )

HSP 1 Score: 260.4 bits (664), Expect = 3.1e-69
Identity = 223/534 (41.76%), Postives = 290/534 (54.31%), Query Frame = 0

Query: 1   MDEYPSKRVANGYTSRRGPSAVMRDTANSRDQDG--KYCSRIGCSGRLNSPRSTRSSYSE 60
           MD +  KR +     R+    V+ +    +D       CSRIGCS R++S +        
Sbjct: 1   MDGFKGKRTSRPIMPRKASGLVLHENMKKKDDKSVVPICSRIGCSSRVSSTKG--DLIDH 60

Query: 61  KLKSPLQSFRTSSSGKEIAGSSSRTYYAVRGSKK-SVAETQRKRPTQLETDSSETSSTQD 120
           K K+ + SFR+  SGKE  GSSSR+     G+KK S    +R+  + L+ DSSE+SS  +
Sbjct: 61  KAKATVSSFRSPLSGKETVGSSSRSMSGFGGTKKASKVIGRRQLSSLLDMDSSESSSVNE 120

Query: 121 DSEISQ-GIPSNERIRKGLHVSLKSSDSTDDTVMEPRSSSVAPNTRGR-RDFNQGSGMRN 180
           DS  S+  +P  +     + V  +SS S  + V E  SSS     RG  R  +Q   + +
Sbjct: 121 DSPTSERSLPRGKTKESTISVHSESSVS-GEVVTEAGSSS-----RGTGRSIHQRPDLVS 180

Query: 181 KDTPASSSVLMGSKSSRPTSRGGADPNLSKLKDTAKKRNSEA-ESSSTRGKKMN---GSS 240
           +D   S+S               A  N + L+D   K  S+   S+ST  +K N     +
Sbjct: 181 RDARVSNS----------EQNARASVNKNGLRDLRNKSGSDVLPSNSTPTRKSNIFRKKT 240

Query: 241 SERRITSSGYGVSISGS-RGAKNGTSNRENGIASVRSRTSINGTS-RARSQSSRPDRNST 300
           S+   +SS  G    GS  G KN +S + NGI     R + N  S R  S  S   R ST
Sbjct: 241 SDGESSSSSRGNKTEGSVVGGKNISSPQGNGITMSEPRRNRNLPSVRDNSVVSSSTRRST 300

Query: 301 SLH--ESRSVIYQESQDHQPESSVDNNAHEVSTELISDHPISYRRSDSMNENILS-HRPA 360
             +    R+      Q  +P +  D N    S E       SY R  S N  + S   P 
Sbjct: 301 GYYGRTGRAGAVATLQAPRPPTRADLNPSR-SAEASRSPLNSYSRPISSNGRLRSLMMPG 360

Query: 361 SPAEIGLTRSLTTRDSFRHY---VIAEVLLALERIEHEEELTYEQATLLETNLFLSGL-N 420
           SP+E GL+RSL  RD+FR Y    +AEVLLALERIE +EELTYEQ  +LETNLFL+G+ +
Sbjct: 361 SPSEAGLSRSLMNRDTFRRYNMNGVAEVLLALERIEQDEELTYEQLAVLETNLFLNGMSS 420

Query: 421 VYDQHRDMRLDIDNMSYEELLALEERMGTVSTAVTEEALSQCLNRSIYQSTHAEDTIAGG 480
            +DQHRDMRLDIDNMSYEELLALEE+MGTVSTA++EEAL + L  SIY+     D I   
Sbjct: 421 FHDQHRDMRLDIDNMSYEELLALEEKMGTVSTALSEEALLKSLKSSIYRPNDESDDIC-- 480

Query: 481 SEYEDGVKCCICQEEYSNGDEVGMLRCEHTYHEGCIQQWLQLKNWCPICKASVE 517
              +D VKC ICQEEY +GDEVG L C+H YH  C QQWL++KNWCPICK S E
Sbjct: 481 LNKDDDVKCSICQEEYVDGDEVGTLPCQHKYHVSCAQQWLRMKNWCPICKTSAE 513

BLAST of Carg08313 vs. TAIR 10
Match: AT5G24870.2 (RING/U-box superfamily protein )

HSP 1 Score: 260.4 bits (664), Expect = 3.1e-69
Identity = 223/534 (41.76%), Postives = 290/534 (54.31%), Query Frame = 0

Query: 1   MDEYPSKRVANGYTSRRGPSAVMRDTANSRDQDG--KYCSRIGCSGRLNSPRSTRSSYSE 60
           MD +  KR +     R+    V+ +    +D       CSRIGCS R++S +        
Sbjct: 1   MDGFKGKRTSRPIMPRKASGLVLHENMKKKDDKSVVPICSRIGCSSRVSSTKG--DLIDH 60

Query: 61  KLKSPLQSFRTSSSGKEIAGSSSRTYYAVRGSKK-SVAETQRKRPTQLETDSSETSSTQD 120
           K K+ + SFR+  SGKE  GSSSR+     G+KK S    +R+  + L+ DSSE+SS  +
Sbjct: 61  KAKATVSSFRSPLSGKETVGSSSRSMSGFGGTKKASKVIGRRQLSSLLDMDSSESSSVNE 120

Query: 121 DSEISQ-GIPSNERIRKGLHVSLKSSDSTDDTVMEPRSSSVAPNTRGR-RDFNQGSGMRN 180
           DS  S+  +P  +     + V  +SS S  + V E  SSS     RG  R  +Q   + +
Sbjct: 121 DSPTSERSLPRGKTKESTISVHSESSVS-GEVVTEAGSSS-----RGTGRSIHQRPDLVS 180

Query: 181 KDTPASSSVLMGSKSSRPTSRGGADPNLSKLKDTAKKRNSEA-ESSSTRGKKMN---GSS 240
           +D   S+S               A  N + L+D   K  S+   S+ST  +K N     +
Sbjct: 181 RDARVSNS----------EQNARASVNKNGLRDLRNKSGSDVLPSNSTPTRKSNIFRKKT 240

Query: 241 SERRITSSGYGVSISGS-RGAKNGTSNRENGIASVRSRTSINGTS-RARSQSSRPDRNST 300
           S+   +SS  G    GS  G KN +S + NGI     R + N  S R  S  S   R ST
Sbjct: 241 SDGESSSSSRGNKTEGSVVGGKNISSPQGNGITMSEPRRNRNLPSVRDNSVVSSSTRRST 300

Query: 301 SLH--ESRSVIYQESQDHQPESSVDNNAHEVSTELISDHPISYRRSDSMNENILS-HRPA 360
             +    R+      Q  +P +  D N    S E       SY R  S N  + S   P 
Sbjct: 301 GYYGRTGRAGAVATLQAPRPPTRADLNPSR-SAEASRSPLNSYSRPISSNGRLRSLMMPG 360

Query: 361 SPAEIGLTRSLTTRDSFRHY---VIAEVLLALERIEHEEELTYEQATLLETNLFLSGL-N 420
           SP+E GL+RSL  RD+FR Y    +AEVLLALERIE +EELTYEQ  +LETNLFL+G+ +
Sbjct: 361 SPSEAGLSRSLMNRDTFRRYNMNGVAEVLLALERIEQDEELTYEQLAVLETNLFLNGMSS 420

Query: 421 VYDQHRDMRLDIDNMSYEELLALEERMGTVSTAVTEEALSQCLNRSIYQSTHAEDTIAGG 480
            +DQHRDMRLDIDNMSYEELLALEE+MGTVSTA++EEAL + L  SIY+     D I   
Sbjct: 421 FHDQHRDMRLDIDNMSYEELLALEEKMGTVSTALSEEALLKSLKSSIYRPNDESDDIC-- 480

Query: 481 SEYEDGVKCCICQEEYSNGDEVGMLRCEHTYHEGCIQQWLQLKNWCPICKASVE 517
              +D VKC ICQEEY +GDEVG L C+H YH  C QQWL++KNWCPICK S E
Sbjct: 481 LNKDDDVKCSICQEEYVDGDEVGTLPCQHKYHVSCAQQWLRMKNWCPICKTSAE 513

BLAST of Carg08313 vs. TAIR 10
Match: AT5G10650.1 (RING/U-box superfamily protein )

HSP 1 Score: 238.0 bits (606), Expect = 1.7e-62
Identity = 203/539 (37.66%), Postives = 287/539 (53.25%), Query Frame = 0

Query: 16  RRGPSAVMRDTANSRD-QDGKYCSRIGCSGRLNSPRSTR-SSYSEKLKSPLQSFRTSSSG 75
           R+     +R+  N  D ++  +CSR+GC+ ++ S + +R  S     K  L    ++ + 
Sbjct: 17  RKASGLTLRENMNKTDGKNVPFCSRVGCTAKVTSTKRSRIGSTDNNTKVGLPPVPSTLNR 76

Query: 76  KEIAGSSSRTYYAVRGSKKSVAETQRKRP-TQLETDSSETSSTQDDSEISQ-GIPSNERI 135
           KEI GSSSRT       +K    T R++P + L+T+SSETS   DD   ++  +P  +  
Sbjct: 77  KEIVGSSSRTPGGFGYLRKPAKVTARRQPSSSLDTESSETSCIHDDPAATEPTLPRQKTK 136

Query: 136 RKGLHVSLKSSDSTDDTVMEPRSSSVAPNTRG-RRDFNQGSGMRNKDTPASSSVLMGSKS 195
           R  ++V  +S+ S +  + +  SSS     RG  R  +  S +  +D     SV   S +
Sbjct: 137 RVTINVHPQSAVSREVVITKAGSSS-----RGTSRISHPKSELGTRDALTGPSVSTSSGN 196

Query: 196 SRPTSRGG-------------------ADPNLSKLKDTAKKRNSEAESS-STRGKKMN-- 255
           S  T RGG                    + N +      KK+N++ ESS S++G K +  
Sbjct: 197 SEHTVRGGLSRHRLRNLSCNSVSDVLPTNSNSATKISVTKKKNADGESSLSSKGSKTSVL 256

Query: 256 GSSSERRITSSGYGVSISGSRGAKNGTSNRENGIASVR-SRTSINGTSRARSQSSRPDRN 315
                 +I+S G GV++S +R        R   + S+R S T ++   R      R +R 
Sbjct: 257 VPKVRNQISSHGNGVTVSDNR--------RNRVVPSIRDSSTVVSNGCRRAGYFGRSERL 316

Query: 316 STSLHESRSVIYQESQDHQPESSVDNNAHEVSTELISDHPISYRRSD--SMNENILSHRP 375
             +   + S      Q   P +  D N             +S+  S+  S    + S+ P
Sbjct: 317 GATASSATS-----RQMPHPTTPTDPNP-----------SLSFCPSNIYSSTGRVHSNMP 376

Query: 376 ASPAEIGLTRSLTTRDSFRHY---VIAEVLLALERIEHEEELTYEQATLLETNLFLSGL- 435
            SP E   + SL  RD   HY    IAEVLLALERIEH+EELTYEQ   +ETNLF SG+ 
Sbjct: 377 GSPTEADPSSSLVNRDGLSHYNMNGIAEVLLALERIEHDEELTYEQLASIETNLFSSGMF 436

Query: 436 NVYDQHRDMRLDIDNMSYEELLALEERMGTVSTAVTEEALSQCLNRSIYQSTHAEDTIAG 495
             YDQHRDMRLDIDNMSYEELLAL ++MGTVSTA++EEALS+ L +SIYQ T  ++T + 
Sbjct: 437 RFYDQHRDMRLDIDNMSYEELLALGDKMGTVSTALSEEALSRSLKQSIYQET--DETGSI 496

Query: 496 GSEYEDGVKCCICQEEYSNGDEVGMLRCEHTYHEGCIQQWLQLKNWCPICKASVEEPAS 521
               +D +KC ICQEEY +GDE+G + C+H YH  C+QQWL++KNWCPICK S EE  S
Sbjct: 497 SLYKDDDIKCSICQEEYVDGDELGTIPCQHMYHVSCVQQWLRMKNWCPICKTSAEEEKS 524

BLAST of Carg08313 vs. TAIR 10
Match: AT5G10650.2 (RING/U-box superfamily protein )

HSP 1 Score: 238.0 bits (606), Expect = 1.7e-62
Identity = 203/539 (37.66%), Postives = 287/539 (53.25%), Query Frame = 0

Query: 16  RRGPSAVMRDTANSRD-QDGKYCSRIGCSGRLNSPRSTR-SSYSEKLKSPLQSFRTSSSG 75
           R+     +R+  N  D ++  +CSR+GC+ ++ S + +R  S     K  L    ++ + 
Sbjct: 17  RKASGLTLRENMNKTDGKNVPFCSRVGCTAKVTSTKRSRIGSTDNNTKVGLPPVPSTLNR 76

Query: 76  KEIAGSSSRTYYAVRGSKKSVAETQRKRP-TQLETDSSETSSTQDDSEISQ-GIPSNERI 135
           KEI GSSSRT       +K    T R++P + L+T+SSETS   DD   ++  +P  +  
Sbjct: 77  KEIVGSSSRTPGGFGYLRKPAKVTARRQPSSSLDTESSETSCIHDDPAATEPTLPRQKTK 136

Query: 136 RKGLHVSLKSSDSTDDTVMEPRSSSVAPNTRG-RRDFNQGSGMRNKDTPASSSVLMGSKS 195
           R  ++V  +S+ S +  + +  SSS     RG  R  +  S +  +D     SV   S +
Sbjct: 137 RVTINVHPQSAVSREVVITKAGSSS-----RGTSRISHPKSELGTRDALTGPSVSTSSGN 196

Query: 196 SRPTSRGG-------------------ADPNLSKLKDTAKKRNSEAESS-STRGKKMN-- 255
           S  T RGG                    + N +      KK+N++ ESS S++G K +  
Sbjct: 197 SEHTVRGGLSRHRLRNLSCNSVSDVLPTNSNSATKISVTKKKNADGESSLSSKGSKTSVL 256

Query: 256 GSSSERRITSSGYGVSISGSRGAKNGTSNRENGIASVR-SRTSINGTSRARSQSSRPDRN 315
                 +I+S G GV++S +R        R   + S+R S T ++   R      R +R 
Sbjct: 257 VPKVRNQISSHGNGVTVSDNR--------RNRVVPSIRDSSTVVSNGCRRAGYFGRSERL 316

Query: 316 STSLHESRSVIYQESQDHQPESSVDNNAHEVSTELISDHPISYRRSD--SMNENILSHRP 375
             +   + S      Q   P +  D N             +S+  S+  S    + S+ P
Sbjct: 317 GATASSATS-----RQMPHPTTPTDPNP-----------SLSFCPSNIYSSTGRVHSNMP 376

Query: 376 ASPAEIGLTRSLTTRDSFRHY---VIAEVLLALERIEHEEELTYEQATLLETNLFLSGL- 435
            SP E   + SL  RD   HY    IAEVLLALERIEH+EELTYEQ   +ETNLF SG+ 
Sbjct: 377 GSPTEADPSSSLVNRDGLSHYNMNGIAEVLLALERIEHDEELTYEQLASIETNLFSSGMF 436

Query: 436 NVYDQHRDMRLDIDNMSYEELLALEERMGTVSTAVTEEALSQCLNRSIYQSTHAEDTIAG 495
             YDQHRDMRLDIDNMSYEELLAL ++MGTVSTA++EEALS+ L +SIYQ T  ++T + 
Sbjct: 437 RFYDQHRDMRLDIDNMSYEELLALGDKMGTVSTALSEEALSRSLKQSIYQET--DETGSI 496

Query: 496 GSEYEDGVKCCICQEEYSNGDEVGMLRCEHTYHEGCIQQWLQLKNWCPICKASVEEPAS 521
               +D +KC ICQEEY +GDE+G + C+H YH  C+QQWL++KNWCPICK S EE  S
Sbjct: 497 SLYKDDDIKCSICQEEYVDGDELGTIPCQHMYHVSCVQQWLRMKNWCPICKTSAEEEKS 524

BLAST of Carg08313 vs. TAIR 10
Match: AT4G31450.1 (RING/U-box superfamily protein )

HSP 1 Score: 213.8 bits (543), Expect = 3.4e-55
Identity = 201/539 (37.29%), Postives = 273/539 (50.65%), Query Frame = 0

Query: 1   MDEYPSKRVANGY-TSRRGPSAVMRDTANSRDQDGKYCSRIGCSGRLNSPRSTR--SSYS 60
           MD + SKR       S++    V RD  NS       C    CS    S   TR   S  
Sbjct: 1   MDRWSSKRAMEARPDSKKKGGVVFRDRFNSNS-----CKVPICSDEKKSMNFTRFVGSSD 60

Query: 61  EKLKSPLQSFRTSSSGKEIAGSSSRTYYAVRGSKKSVAETQRKRPTQLETDSSETSSTQD 120
           +K KS L ++R+S +GKE+ G+SS+   +   S  SV   +++  +Q+  DSSE+S   +
Sbjct: 61  KKEKSVLSTYRSSPNGKEVIGTSSKICIS---SSSSVKSGEKQPFSQIAIDSSESSRGSE 120

Query: 121 D---SEISQGIPSNERIRKGLHVSLKSSDSTDDTVMEPRSSSVAPNTRGRRDFNQGSGMR 180
           D   SEI Q +P     R+  +  +     T     E   S +  ++R +R F Q  G+ 
Sbjct: 121 DEVESEILQ-VPLGRDKRRMNNKLIYGKVITP----EAECSKLPSSSRIKRGFRQRFGLS 180

Query: 181 NKD--TPASSSVLMGSKSSRPTSRG------GADPNLSKLKDTAKKRNSEAESSST---R 240
            ++     S      ++   P   G      G D  LS+  DT  K     ESSS+   R
Sbjct: 181 KQEFHPGPSGQSTSANRGCSPLLSGVIPSGFGLDKRLSRKADTISKTKVYGESSSSSSAR 240

Query: 241 GKKMNGSSS-ERRITSSGYGVSISGSRGAKNGTSNRENGIASVRSRTSINGTSRARSQSS 300
           GK +      E R  S     S+S SR A++   + +N +ASV S+   N  +     S 
Sbjct: 241 GKNVTEPPPVEVRRRSFNPRGSVSDSRRARHCILDDDNDVASVGSQRLANRNNSRIRGSG 300

Query: 301 RPDRNSTSLHESRSVIYQESQDHQPESSVDNNAHEVSTELISDHPISYRRSDSMNENILS 360
           R   +S +  E            Q E+S + N+  VS EL S  P               
Sbjct: 301 RDGLSSVTAAEM----------SQTETSNNLNS-PVSLELFSGFP--------------- 360

Query: 361 HRPASPAEIGLTRSLTTRDSFRHY---VIAEVLLALERIEHEEELTYEQATLLETNLFLS 420
                  E GL+ SL + DSFR Y    I+E+L  L+RIE + EL YE   ++ET L L 
Sbjct: 361 -------EFGLSGSLLSHDSFRSYNLDGISEILPELDRIEQDIELNYEDLLIMETGLLLG 420

Query: 421 GLNVYDQHRDMRLDIDNMSYEELLALEERMGTVSTAVTEEALSQCLNRSIYQSTHAE--D 480
           GL+ +DQHRDMRLDIDNMSYEELLALEER+GTVSTA+TEEA+S+CL  SIYQ        
Sbjct: 421 GLSFHDQHRDMRLDIDNMSYEELLALEERIGTVSTALTEEAISKCLKTSIYQMKPLSYGS 480

Query: 481 TIAGGSEYEDGVKCCICQEEYSNGDEVGMLRCEHTYHEGCIQQWLQLKNWCPICKASVE 517
                S+ ++  KC ICQEEY+ GDEVG L CEHTYH  C+Q+WL++K+WCPICKA+ E
Sbjct: 481 ITKSPSDNKEDAKCSICQEEYTIGDEVGRLHCEHTYHVKCVQEWLRIKSWCPICKATAE 493