Carg08309 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg08309
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Aminoacyl-tRNA hydrolase
Location	Carg_Chr05: 3322812 .. 3324090 (+)
RNA-Seq Expression	Carg08309
Synteny	Carg08309

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTTTCATGTGCTACCTTGTGTGAAGATATCATGATTTCTGTAATTTCAGCGTCTCCATTCACTTTCTCCATTTCCAAATCCACGAAGCTCTGTTCGTGTTCGAGGTCTAGGGTTTGGCCCTCTTCTTCACTCAGTCTCCGCTCGCGTAACCGAAATTCGAATCTGTTTAGCTCCGCTTCTATGAGTGAATCCGCTGGAAGTAATTCCGCTCCTCCACCGGACGACGGCGTGGAGAAGACAGCCGATGTTTTGATTCAGTACGTGGTGCTCCGGAGAGATCTGATCGATTCGTGGCCTCTTGGAAGCGTCGTAACTCAGGGATGCCATGCTTCAGTGTCCGCTATTTGGTTGAACAAAGACGATCCTCACACTTCCGAGTACTGTAGTCCACACAATATCGATTCTATGCACAAGGTTAGTCGAACTTTTGATTCCATTTTTAAATCTGATTGTGAAGTCTGGTGCCTCGAACTCGGACGGACAATGTAGTATTCGTGTGCAGCCTTGAACCTTTTCTGTTGATAAGCTGAAAATTTGCTTCGTTGGTGCGCGCGGTGTTAAGTCGGTCTAGATGAGACATTCTGAGCAATTTGGAACTTTCGTGTTGTGGTTCTCGTCGCTTGTTGATTTGTTTAATTCTGGAAAGAACATTTTGCATCTATCAAGCATTTTGGAAGAACAATTGTTTACTGGAAAACTATTTTTGTTTGGGTTCTTGTGTTCTCCATTTGAAGTACATTTTTGCTATTCTGAAGTTCGAGTCCTCCTTGCTCGTTTTGTTTGCTGAATGTTCTGATTTTTCGACCTAATATTCATACGGTTGTATCTGTCTAGGCTCTCCACCGTATTCGTAGAGTCGTAGGGAAAAGAGAGGGTTGTTTTTTTTAGTTCTATAGCTTCCAAGGAATTTTTCACCTCCATTTCGCATTGGCCTGTGTAACCAGCCCCATAGTTAATGCTAACCTCATCATTTCAAGCTTGCAAAACGATTGAACTGCCAATTGGAAGTTTCCTTGCTCATCAGATTCTTATCGTGTGCAACTTAATTAGTTGAAATTACAGTTGTTCTACATTTCATTGATCCCTTAAGTCGTTCTGGACTTCTTGATCCCTGAAGTCAGTACTGACTTTATTTAACTTTATGTAGGTTACTCTTGAGGTGAAGGGAGAAACTCAGATTGTCAACTTGTCGGAGAAACTAAAAGCAAGTTCAATCGCCCACAAACTATGGATAGAACAACCAGAGCATCCCAATTCTTGTCGTGCAACAACTTAA

mRNA sequence

ATGGTTTCATGTGCTACCTTGTGTGAAGATATCATGATTTCTGTAATTTCAGCGTCTCCATTCACTTTCTCCATTTCCAAATCCACGAAGCTCTGTTCGTGTTCGAGGTCTAGGGTTTGGCCCTCTTCTTCACTCAGTCTCCGCTCGCGTAACCGAAATTCGAATCTGTTTAGCTCCGCTTCTATGAGTGAATCCGCTGGAAGTAATTCCGCTCCTCCACCGGACGACGGCGTGGAGAAGACAGCCGATGTTTTGATTCAGTACGTGGTGCTCCGGAGAGATCTGATCGATTCGTGGCCTCTTGGAAGCGTCGTAACTCAGGGATGCCATGCTTCAGTGTCCGCTATTTGGTTGAACAAAGACGATCCTCACACTTCCGAGTACTGTAGTCCACACAATATCGATTCTATGCACAAGGTTACTCTTGAGGTGAAGGGAGAAACTCAGATTGTCAACTTGTCGGAGAAACTAAAAGCAAGTTCAATCGCCCACAAACTATGGATAGAACAACCAGAGCATCCCAATTCTTGTCGTGCAACAACTTAA

Coding sequence (CDS)

ATGGTTTCATGTGCTACCTTGTGTGAAGATATCATGATTTCTGTAATTTCAGCGTCTCCATTCACTTTCTCCATTTCCAAATCCACGAAGCTCTGTTCGTGTTCGAGGTCTAGGGTTTGGCCCTCTTCTTCACTCAGTCTCCGCTCGCGTAACCGAAATTCGAATCTGTTTAGCTCCGCTTCTATGAGTGAATCCGCTGGAAGTAATTCCGCTCCTCCACCGGACGACGGCGTGGAGAAGACAGCCGATGTTTTGATTCAGTACGTGGTGCTCCGGAGAGATCTGATCGATTCGTGGCCTCTTGGAAGCGTCGTAACTCAGGGATGCCATGCTTCAGTGTCCGCTATTTGGTTGAACAAAGACGATCCTCACACTTCCGAGTACTGTAGTCCACACAATATCGATTCTATGCACAAGGTTACTCTTGAGGTGAAGGGAGAAACTCAGATTGTCAACTTGTCGGAGAAACTAAAAGCAAGTTCAATCGCCCACAAACTATGGATAGAACAACCAGAGCATCCCAATTCTTGTCGTGCAACAACTTAA

Protein sequence

MVSCATLCEDIMISVISASPFTFSISKSTKLCSCSRSRVWPSSSLSLRSRNRNSNLFSSASMSESAGSNSAPPPDDGVEKTADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPHTSEYCSPHNIDSMHKVTLEVKGETQIVNLSEKLKASSIAHKLWIEQPEHPNSCRATT

Homology

[Close]

BLAST of Carg08309 vs. NCBI nr
Match: KAG7029746.1 (putative peptidyl-tRNA hydrolase PTRHD1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 356.7 bits (914), Expect = 1.2e-94
Identity = 181/181 (100.00%), Postives = 181/181 (100.00%), Query Frame = 0


Query: 1   MVSCATLCEDIMISVISASPFTFSISKSTKLCSCSRSRVWPSSSLSLRSRNRNSNLFSSA 60
           MVSCATLCEDIMISVISASPFTFSISKSTKLCSCSRSRVWPSSSLSLRSRNRNSNLFSSA
Sbjct: 1   MVSCATLCEDIMISVISASPFTFSISKSTKLCSCSRSRVWPSSSLSLRSRNRNSNLFSSA 60

Query: 61  SMSESAGSNSAPPPDDGVEKTADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNK 120
           SMSESAGSNSAPPPDDGVEKTADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNK
Sbjct: 61  SMSESAGSNSAPPPDDGVEKTADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNK 120

Query: 121 DDPHTSEYCSPHNIDSMHKVTLEVKGETQIVNLSEKLKASSIAHKLWIEQPEHPNSCRAT 180
           DDPHTSEYCSPHNIDSMHKVTLEVKGETQIVNLSEKLKASSIAHKLWIEQPEHPNSCRAT
Sbjct: 121 DDPHTSEYCSPHNIDSMHKVTLEVKGETQIVNLSEKLKASSIAHKLWIEQPEHPNSCRAT 180

Query: 181 T 182
           T
Sbjct: 181 T 181

[Close]

BLAST of Carg08309 vs. NCBI nr
Match: XP_023546189.1 (putative peptidyl-tRNA hydrolase PTRHD1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 337.8 bits (865), Expect = 5.7e-89
Identity = 173/180 (96.11%), Postives = 176/180 (97.78%), Query Frame = 0


Query: 1   MVSCATLCEDIMISVISASPFTFSISKSTKLCSCSRSRVWPSSSLSLRSRNRNSNLFSSA 60
           MVSCATLCEDIMISVISASPFTFSISKSTKLCSCSRSRVWPSSSLSLRSRNRNSNLFSSA
Sbjct: 1   MVSCATLCEDIMISVISASPFTFSISKSTKLCSCSRSRVWPSSSLSLRSRNRNSNLFSSA 60

Query: 61  SMSESAGSNSAPPPDDGVEKTADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNK 120
           SMSESA SNSAPPPDDGVEKTADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSA+WLNK
Sbjct: 61  SMSESAESNSAPPPDDGVEKTADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAVWLNK 120

Query: 121 DDPHTSEYCSPHNIDSMHKVTLEVKGETQIVNLSEKLKASSIAHKLWIEQPEHPNSCRAT 180
           DDPHTSEYCSPHNIDSMHKVTLEVKGETQIVNLSEKLKASSIAHKLWIEQPE+  +C AT
Sbjct: 121 DDPHTSEYCSPHNIDSMHKVTLEVKGETQIVNLSEKLKASSIAHKLWIEQPENIPTCLAT 180

[Close]

BLAST of Carg08309 vs. NCBI nr
Match: KAG6598808.1 (putative peptidyl-tRNA hydrolase PTRHD1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 309.3 bits (791), Expect = 2.2e-80
Identity = 159/159 (100.00%), Postives = 159/159 (100.00%), Query Frame = 0


Query: 1   MVSCATLCEDIMISVISASPFTFSISKSTKLCSCSRSRVWPSSSLSLRSRNRNSNLFSSA 60
           MVSCATLCEDIMISVISASPFTFSISKSTKLCSCSRSRVWPSSSLSLRSRNRNSNLFSSA
Sbjct: 1   MVSCATLCEDIMISVISASPFTFSISKSTKLCSCSRSRVWPSSSLSLRSRNRNSNLFSSA 60

Query: 61  SMSESAGSNSAPPPDDGVEKTADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNK 120
           SMSESAGSNSAPPPDDGVEKTADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNK
Sbjct: 61  SMSESAGSNSAPPPDDGVEKTADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNK 120

Query: 121 DDPHTSEYCSPHNIDSMHKVTLEVKGETQIVNLSEKLKA 160
           DDPHTSEYCSPHNIDSMHKVTLEVKGETQIVNLSEKLKA
Sbjct: 121 DDPHTSEYCSPHNIDSMHKVTLEVKGETQIVNLSEKLKA 159

[Close]

BLAST of Carg08309 vs. NCBI nr
Match: XP_022962062.1 (putative peptidyl-tRNA hydrolase PTRHD1 [Cucurbita moschata])

HSP 1 Score: 303.9 bits (777), Expect = 9.2e-79
Identity = 156/169 (92.31%), Postives = 161/169 (95.27%), Query Frame = 0


Query: 12  MISVISASPFTFSISKSTKLCSCSRSRVWPSSSLSLRSRNRNSNLFSSASMSESAGSNSA 71
           MISVISAS FTFSISKSTKLCSCSRSRVWPSSSLSLRSRNRNSNLFSS SMSESA SNSA
Sbjct: 1   MISVISASRFTFSISKSTKLCSCSRSRVWPSSSLSLRSRNRNSNLFSSDSMSESAESNSA 60

Query: 72  PPPDDGVEKTADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPHTSEYCSP 131
           PPPDD VEKTADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSA+WLNKDDPHT EYCSP
Sbjct: 61  PPPDDSVEKTADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAVWLNKDDPHTFEYCSP 120

Query: 132 HNIDSMHKVTLEVKGETQIVNLSEKLKASSIAHKLWIEQPEHPNSCRAT 181
           HNIDSMHKVTLEVKGETQIVNLSEKL+A+SIAHKLWIEQPE+  +C AT
Sbjct: 121 HNIDSMHKVTLEVKGETQIVNLSEKLRANSIAHKLWIEQPENIPTCLAT 169

[Close]

BLAST of Carg08309 vs. NCBI nr
Match: XP_022996792.1 (putative peptidyl-tRNA hydrolase PTRHD1 [Cucurbita maxima])

HSP 1 Score: 301.6 bits (771), Expect = 4.6e-78
Identity = 157/169 (92.90%), Postives = 160/169 (94.67%), Query Frame = 0


Query: 12  MISVISASPFTFSISKSTKLCSCSRSRVWPSSSLSLRSRNRNSNLFSSASMSESAGSNSA 71
           MISVISAS FTFSISKSTKLCSCSRSRVWPSSSLSLRSRNRNSNLFSSASMSESA SNSA
Sbjct: 1   MISVISASRFTFSISKSTKLCSCSRSRVWPSSSLSLRSRNRNSNLFSSASMSESAESNSA 60

Query: 72  PPPDDGVEKTADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPHTSEYCSP 131
           PPPDD VEK ADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDP TSEYCSP
Sbjct: 61  PPPDDIVEKAADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPQTSEYCSP 120

Query: 132 HNIDSMHKVTLEVKGETQIVNLSEKLKASSIAHKLWIEQPEHPNSCRAT 181
           HNIDSMHKVTLEVKGETQIVNLSEKLK +SIAHKLWIEQPE+  +C AT
Sbjct: 121 HNIDSMHKVTLEVKGETQIVNLSEKLKENSIAHKLWIEQPENIPTCLAT 169

[Close]

BLAST of Carg08309 vs. ExPASy Swiss-Prot
Match: Q3SZ85 (Putative peptidyl-tRNA hydrolase PTRHD1 OS=Bos taurus OX=9913 GN=PTRHD1 PE=2 SV=2)

HSP 1 Score: 94.4 bits (233), Expect = 1.5e-18
Identity = 46/99 (46.46%), Postives = 66/99 (66.67%), Query Frame = 0


Query: 84  VLIQYVVLRRDLID---SWPLGSVVTQGCHASVSAIWLNKDDPHTSEYCSPHNIDSMHKV 143
           +L+QY+VLR+DL     SWP G+VV Q CHA+ +A+ L++D PHT+ Y     ++ M KV
Sbjct: 24  ILVQYLVLRKDLTQPPFSWPAGAVVAQACHAATAALHLHRDHPHTAAYL--RELERMRKV 83

Query: 144 TLEVKGETQIVNLSEKLKASSIAHKLWIEQPEHPNSCRA 180
            LE   ET +  L+E L+  +I HKLW+EQPE+  +C A
Sbjct: 84  VLEAPDETTLKLLAETLQQKNIDHKLWMEQPENIPTCIA 120

[Close]

BLAST of Carg08309 vs. ExPASy Swiss-Prot
Match: D3Z4S3 (Putative peptidyl-tRNA hydrolase PTRHD1 OS=Mus musculus OX=10090 GN=Ptrhd1 PE=1 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 1.2e-17
Identity = 47/106 (44.34%), Postives = 67/106 (63.21%), Query Frame = 0


Query: 77  GVEKTADVLIQYVVLRRDLID---SWPLGSVVTQGCHASVSAIWLNKDDPHTSEYCSPHN 136
           GVE    +L+QY+VLR+DL     SWP G++V Q CHA+ +A+ L++D PHT+ Y     
Sbjct: 19  GVE--PQILVQYLVLRKDLSQAPFSWPTGALVAQACHAATAALHLHRDHPHTAAYL--RE 78

Query: 137 IDSMHKVTLEVKGETQIVNLSEKLKASSIAHKLWIEQPEHPNSCRA 180
           +  M KV LE   ET +  L+E L+  +I H LW+EQPE+  +C A
Sbjct: 79  LGRMRKVVLEAADETTLKELAETLQQKNIDHTLWLEQPENIATCIA 120

[Close]

BLAST of Carg08309 vs. ExPASy Swiss-Prot
Match: Q6GMV3 (Putative peptidyl-tRNA hydrolase PTRHD1 OS=Homo sapiens OX=9606 GN=PTRHD1 PE=1 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 4.7e-17
Identity = 44/99 (44.44%), Postives = 63/99 (63.64%), Query Frame = 0


Query: 84  VLIQYVVLRRDLID---SWPLGSVVTQGCHASVSAIWLNKDDPHTSEYCSPHNIDSMHKV 143
           VL+QY+VLR+DL     SWP G++V Q CHA+ +A+  ++D PHT+ Y     +  M KV
Sbjct: 24  VLVQYLVLRKDLSQAPFSWPAGALVAQACHAATAALHTHRDHPHTAAYL--QELGRMRKV 83

Query: 144 TLEVKGETQIVNLSEKLKASSIAHKLWIEQPEHPNSCRA 180
            LE   ET +  L+E L+  +I H LW+EQPE+  +C A
Sbjct: 84  VLEAPDETTLKELAETLQQKNIDHMLWLEQPENIATCIA 120

[Close]

BLAST of Carg08309 vs. ExPASy TrEMBL
Match: A0A6J1HBR1 (Aminoacyl-tRNA hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111462634 PE=4 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 4.5e-79
Identity = 156/169 (92.31%), Postives = 161/169 (95.27%), Query Frame = 0


Query: 12  MISVISASPFTFSISKSTKLCSCSRSRVWPSSSLSLRSRNRNSNLFSSASMSESAGSNSA 71
           MISVISAS FTFSISKSTKLCSCSRSRVWPSSSLSLRSRNRNSNLFSS SMSESA SNSA
Sbjct: 1   MISVISASRFTFSISKSTKLCSCSRSRVWPSSSLSLRSRNRNSNLFSSDSMSESAESNSA 60

Query: 72  PPPDDGVEKTADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPHTSEYCSP 131
           PPPDD VEKTADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSA+WLNKDDPHT EYCSP
Sbjct: 61  PPPDDSVEKTADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAVWLNKDDPHTFEYCSP 120

Query: 132 HNIDSMHKVTLEVKGETQIVNLSEKLKASSIAHKLWIEQPEHPNSCRAT 181
           HNIDSMHKVTLEVKGETQIVNLSEKL+A+SIAHKLWIEQPE+  +C AT
Sbjct: 121 HNIDSMHKVTLEVKGETQIVNLSEKLRANSIAHKLWIEQPENIPTCLAT 169

[Close]

BLAST of Carg08309 vs. ExPASy TrEMBL
Match: A0A6J1K304 (Aminoacyl-tRNA hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111491924 PE=4 SV=1)

HSP 1 Score: 301.6 bits (771), Expect = 2.2e-78
Identity = 157/169 (92.90%), Postives = 160/169 (94.67%), Query Frame = 0


Query: 12  MISVISASPFTFSISKSTKLCSCSRSRVWPSSSLSLRSRNRNSNLFSSASMSESAGSNSA 71
           MISVISAS FTFSISKSTKLCSCSRSRVWPSSSLSLRSRNRNSNLFSSASMSESA SNSA
Sbjct: 1   MISVISASRFTFSISKSTKLCSCSRSRVWPSSSLSLRSRNRNSNLFSSASMSESAESNSA 60

Query: 72  PPPDDGVEKTADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPHTSEYCSP 131
           PPPDD VEK ADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDP TSEYCSP
Sbjct: 61  PPPDDIVEKAADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPQTSEYCSP 120

Query: 132 HNIDSMHKVTLEVKGETQIVNLSEKLKASSIAHKLWIEQPEHPNSCRAT 181
           HNIDSMHKVTLEVKGETQIVNLSEKLK +SIAHKLWIEQPE+  +C AT
Sbjct: 121 HNIDSMHKVTLEVKGETQIVNLSEKLKENSIAHKLWIEQPENIPTCLAT 169

[Close]

BLAST of Carg08309 vs. ExPASy TrEMBL
Match: A0A0A0LLQ2 (Aminoacyl-tRNA hydrolase OS=Cucumis sativus OX=3659 GN=Csa_2G377990 PE=4 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 6.5e-62
Identity = 132/173 (76.30%), Postives = 143/173 (82.66%), Query Frame = 0


Query: 12  MISVISASPFTFSISKSTKLCSCSRSRVWPSSSLSLRSRNRNSNLFSSASMSESAGSNSA 71
           M  VISAS FT SISKS  LCSC RSRV+PSSSLSL S  R ++ F SASMS+S  +NS 
Sbjct: 1   MTCVISASRFTLSISKSISLCSCPRSRVFPSSSLSLHSSIRKTHPFCSASMSQSVETNSV 60

Query: 72  PPPDD----GVEKTADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPHTSE 131
             PDD      E  ADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPHTS+
Sbjct: 61  STPDDAGAKAEETPADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPHTSD 120

Query: 132 YCSPHNIDSMHKVTLEVKGETQIVNLSEKLKASSIAHKLWIEQPEHPNSCRAT 181
           YC+PHNIDSMHKVTLEVKGETQ+VNLSEKLKA+SI HKLWIEQPE+  +C AT
Sbjct: 121 YCNPHNIDSMHKVTLEVKGETQMVNLSEKLKANSIVHKLWIEQPENIPTCLAT 173

[Close]

BLAST of Carg08309 vs. ExPASy TrEMBL
Match: A0A6J1KJQ6 (Aminoacyl-tRNA hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111496331 PE=4 SV=1)

HSP 1 Score: 243.0 bits (619), Expect = 9.3e-61
Identity = 133/172 (77.33%), Postives = 143/172 (83.14%), Query Frame = 0


Query: 12  MISVISASPFTFSISKSTKLCSCSRSRVWPSSSLSLRSRNRNSNLFSSASMSESAGSNSA 71
           M SVISAS FTFSIS S +L  CS +RVW  SS+ L S N NS  FSSASMSESA SNS 
Sbjct: 1   MASVISASRFTFSISNSARL--CSNARVWLPSSICLLSWNWNSISFSSASMSESAESNSV 60

Query: 72  PPPDDG---VEKTADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPHTSEY 131
            P DDG    E+ ADVL+QYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPHTSEY
Sbjct: 61  LPLDDGGAKAEEAADVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPHTSEY 120

Query: 132 CSPHNIDSMHKVTLEVKGETQIVNLSEKLKASSIAHKLWIEQPEHPNSCRAT 181
           CSP NIDSMHKVTLEVKGETQIVNLSEKLKA+SI HKLW+EQPE+  +C A+
Sbjct: 121 CSPQNIDSMHKVTLEVKGETQIVNLSEKLKANSIVHKLWVEQPENIPTCLAS 170

[Close]

BLAST of Carg08309 vs. ExPASy TrEMBL
Match: A0A6J1KTS0 (Aminoacyl-tRNA hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111496331 PE=4 SV=1)

HSP 1 Score: 243.0 bits (619), Expect = 9.3e-61
Identity = 133/172 (77.33%), Postives = 143/172 (83.14%), Query Frame = 0


Query: 12  MISVISASPFTFSISKSTKLCSCSRSRVWPSSSLSLRSRNRNSNLFSSASMSESAGSNSA 71
           M SVISAS FTFSIS S +L  CS +RVW  SS+ L S N NS  FSSASMSESA SNS 
Sbjct: 1   MASVISASRFTFSISNSARL--CSNARVWLPSSICLLSWNWNSISFSSASMSESAESNSV 60

Query: 72  PPPDDG---VEKTADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPHTSEY 131
            P DDG    E+ ADVL+QYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPHTSEY
Sbjct: 61  LPLDDGGAKAEEAADVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPHTSEY 120

Query: 132 CSPHNIDSMHKVTLEVKGETQIVNLSEKLKASSIAHKLWIEQPEHPNSCRAT 181
           CSP NIDSMHKVTLEVKGETQIVNLSEKLKA+SI HKLW+EQPE+  +C A+
Sbjct: 121 CSPQNIDSMHKVTLEVKGETQIVNLSEKLKANSIVHKLWVEQPENIPTCLAS 170

[Close]

BLAST of Carg08309 vs. TAIR 10
Match: AT5G10700.1 (Peptidyl-tRNA hydrolase II (PTH2) family protein )

HSP 1 Score: 163.3 bits (412), Expect = 1.8e-40
Identity = 91/169 (53.85%), Postives = 112/169 (66.27%), Query Frame = 0


Query: 12  MISVISASPFTFSISKSTKLCSCSRSRVWPSSSLSLRSRNRNSNLFSSASMSESAGSNSA 71
           M + + ++ F F I          R R + S   ++R  +      +S SMS+  G   A
Sbjct: 1   MATAVLSAAFRFPIVTRRLSAPAPRIRFYHS---TIRFAHFRRFTTASPSMSQQTGEIDA 60

Query: 72  PPPDDGVEKTADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPHTSEYCSP 131
                  EK  DV++QYVVLRRDLIDSWPLGSVVTQGCHASV+AIW  KDDP T +YC P
Sbjct: 61  VVDASSAEKPDDVVVQYVVLRRDLIDSWPLGSVVTQGCHASVAAIWSFKDDPVTLQYCDP 120

Query: 132 HNIDSMHKVTLEVKGETQIVNLSEKLKASSIAHKLWIEQPEHPNSCRAT 181
            +IDSMHKVTLEVKGETQ++NLSEKLK   I+HKLW+EQPE+  +C AT
Sbjct: 121 QHIDSMHKVTLEVKGETQMMNLSEKLKLGGISHKLWMEQPENIPTCIAT 166

The following BLAST results are available for this feature:

BLAST of Carg08309 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7029746.1	1.2e-94	100.00	putative peptidyl-tRNA hydrolase PTRHD1, partial [Cucurbita argyrosperma subsp. ...	[more]
XP_023546189.1	5.7e-89	96.11	putative peptidyl-tRNA hydrolase PTRHD1 [Cucurbita pepo subsp. pepo]	[more]
KAG6598808.1	2.2e-80	100.00	putative peptidyl-tRNA hydrolase PTRHD1, partial [Cucurbita argyrosperma subsp. ...	[more]
XP_022962062.1	9.2e-79	92.31	putative peptidyl-tRNA hydrolase PTRHD1 [Cucurbita moschata]	[more]
XP_022996792.1	4.6e-78	92.90	putative peptidyl-tRNA hydrolase PTRHD1 [Cucurbita maxima]	[more]

BLAST of Carg08309 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q3SZ85	1.5e-18	46.46	Putative peptidyl-tRNA hydrolase PTRHD1 OS=Bos taurus OX=9913 GN=PTRHD1 PE=2 SV=...	[more]
D3Z4S3	1.2e-17	44.34	Putative peptidyl-tRNA hydrolase PTRHD1 OS=Mus musculus OX=10090 GN=Ptrhd1 PE=1 ...	[more]
Q6GMV3	4.7e-17	44.44	Putative peptidyl-tRNA hydrolase PTRHD1 OS=Homo sapiens OX=9606 GN=PTRHD1 PE=1 S...	[more]

BLAST of Carg08309 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HBR1	4.5e-79	92.31	Aminoacyl-tRNA hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111462634 PE=4 SV=1	[more]
A0A6J1K304	2.2e-78	92.90	Aminoacyl-tRNA hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111491924 PE=4 SV=1	[more]
A0A0A0LLQ2	6.5e-62	76.30	Aminoacyl-tRNA hydrolase OS=Cucumis sativus OX=3659 GN=Csa_2G377990 PE=4 SV=1	[more]
A0A6J1KJQ6	9.3e-61	77.33	Aminoacyl-tRNA hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111496331 PE=4 SV=1	[more]
A0A6J1KTS0	9.3e-61	77.33	Aminoacyl-tRNA hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111496331 PE=4 SV=1	[more]

BLAST of Carg08309 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G10700.1	1.8e-40	53.85	Peptidyl-tRNA hydrolase II (PTH2) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR023476	Peptidyl-tRNA hydrolase II domain superfamily	GENE3D	3.40.1490.10	Bit1	coord: 85..178 e-value: 1.8E-10 score: 42.9
IPR023476	Peptidyl-tRNA hydrolase II domain superfamily	SUPERFAMILY	102462	Peptidyl-tRNA hydrolase II	coord: 86..180
IPR002833	Peptidyl-tRNA hydrolase, PTH2	PFAM	PF01981	PTH2	coord: 87..169 e-value: 4.9E-15 score: 55.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 59..78
IPR042237	Putative peptidyl-tRNA hydrolase PTRHD1	PANTHER	PTHR46194	PEPTIDYL-TRNA HYDROLASE PTRHD1-RELATED	coord: 51..180

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg08309-RA	Carg08309-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0004045	aminoacyl-tRNA hydrolase activity