Carg08302 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg08302
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Ribonuclease H2 subunit B
Location	Carg_Chr05: 3283345 .. 3287757 (+)
RNA-Seq Expression	Carg08302
Synteny	Carg08302

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTGGCGCGCGCGGTTTTTCCAGTTCTCGATTTCGTTTGGTTCCCGCCGTCGACATTCACATTTCCGTTTTCCGGCGAGGTCGGTACTTCATCTCTCTGTGGACGCCGGTGCTCTTCCGACCTTCTTCGTAGGTAAAAATGGTTCTTCGCCTTCTTTTCCTGAGATGAAAATTGTCGCCTCGATTGTTTTGATTCTCATACTCGGAACTGAAACTGGTGTATGCGCAATCTAAGACTAGGTTTTAAGAATGAAGCTCAATGTTACAATTTAGTTCAGATTATTATTTTTTGATGTTACTGTTTCGATGACCGTCAGGGTTGTTCCATGGCTTGGTGGGAAGGCGTCGAGGAAACTCGTATCCTCATTGCCCCTGGTAGGTCCGCGTTTTATTTATTTGTCATGTTCTTGATCTCGCCATTTCTTAAGGTAATCACTTATTACATTTTAATTTTGGAAATTTTGACTAAGTTAGATTTCTCTTGCTCTATTACAGATCATGGGGTAAAAGAAAATGGTACTGGATGCTTGCTTTCACTTCGCCATCCAAAATCAGGTAATTTAAGGAACTGGAGAGAAAATGATAGTTATCTAAATACTTTATTTGGTTAGCTATCCTATTGATCGGTGACCATCTTTCAGCATAACTTTATTGTGGAAAACTGTAAGGAAGTGCAAGCTAATGCTCATCATATTTCTACAAAATCATTCTATTAATTGAGTATATTCGATGTCTGTTGGCAGGAAATACGACATGTTTCTTATATGTAAACGAGTTGCTAGAGGAAATTCACTGGTTTAAGCAGTCATATTCATCGTGGTTTTTGGGAGATTATGTTACTGAAGGTAAGCAACTGTGTCACACTGAAAATCTTTTTGTGGATGCCTTATTTGTTAGACTTACTGCATAACTTCTAATGGTTTGAATCTCCTGAAGATTTTGCTAATTCAAGTATAAAATATTGAAAATAAACTATAAAACACAAGTAATTTTGAATAAATAACTTAATATATATTATTTTGTGTCTACAGATGGTCGTCTGTATTCCGCTACTCCAGTGGATCCTGTATTCATTTTCTTGCCCATTTTCGAGGAAGCTAGAATGAAGGTACTCTCACTGCAAAACTTGTATGATATCATAATTGAGAGATGGAGCATAATTGTGTGCTGCATACAATTATTTTGCATTCTACAATAGTAGTGATGTATGTTTGCGATCTACTTGGAAATTAGAGATACAATTTCAAGTTTTCCCTGGTTGCTTTGGAATTTATATGTCCATTTGTTTATCTTGAAATTGTTGGTCTAATTGTGATGCGTACAGAGCAAGTTAATTATATTGTTCATTTGAACTCATTATAATATATTTATAGTCGCTGAAACTATTAATATTTTCAATTGATTTAATGCTAAATTTTGAGAATTTTATTTCAAATTTCTTGCAGAAGGGAGATGATCCTGGGAAATTCAGGCAATTAGATGACATAATGTTCATCAATGGGTATCCTGGATATCTGCATTTACTGTCTGTTGCAGAGAAGTCTATGCAAGTAGTATGTGAGGTCAAAGGTGGGCATATAGTATATAAAATTAGAAAGGAAAAAAAAAAAAAAAAAAATCAAGCATGTCTAGAATCTATTCTCATTTCTGCTGTCTTGTCTTGTCTCCTGCAGAAATTGGATCTTCTAAGTTTTTTAGGCTTGATGATTCAAAGGTTTTGGCATGGTTATATCACAAGGTTCGTGTTCGTTCTCTCCACTAGAAATTTTCAAAATTGGTCCGTTAAGATGAGAACAATTAACTGTGACATCCTACAATCCCACATTGGTTGGGGAGGAGAACAAAACACCCTTTATAAGGGTGTGGAAACCTTTCCCTAGCAGACGCATTTTAAAAACCTGGAGGGGAAACCTGAAAGGACAAACCCAAAGAGGACAATATCTACTAGCGGTGTGCTTGGGCCGTTACAGATGGTATTAGAGCCAGACACTGGACAATGTGCCAGCGAGGAGACTCTTCCCCGAAGGGGGTAGACACGAGGCGATGTGCCAGTAAGGATGCTGGGCCCCGAAGATGGTGGATTTGGCGGGGGTCCCACATCGATTGGAGAAAGGAACAAGTGCCAGTGAGGACGCTTGGCCTTGAAGGTGGTGGATTGTGACATCCTACAATCCCACATTTGTTGGGGAGGAAAACGAAACACCTTTTATAAGGGTGTGGAAACCTTTCCCTGACAGACACGTTTTAAAAACTTTGAGAAGAAACCCAAAAGGAAAAATCCAAAGAGGACAATATCTGCTAGCGGTGTGTTTGGGCCGTTACATTAACAATAATGCATATCTTGATCAACGTGTTCATAGGTGATTGATGAATCCTGTTTTACATCAGTTTCTAACATGGGAGTATCTAGCTGCCAACTCTTCATGCTGTACTGCTTTTTCCATATAACAGCTACTTTTATTTTCCATAGTATCTTCTGGAAGAGATTTCGCGGTTTCTCTTATTTATTATATTATTTAATTTTATGCTTTTATTGTTGCAGGTATGTCAGCTCAAAAAAACTCTATGCACACTGGATAAAAACTATGCTGCAAGGGAAGAAAAAGATACATGTATATTTCTATAGAGACTTATAACCCCTTTATGCCATTCGTTTTAACACTGCAGCTGGGTTTCTTTCATTTTTATCTTCATCATTATAGTTTCCTATTGTTTAATATCATCTGGCACATCTGAAAAAACGATATAAAGAGGACATCATCAGTAGTTTTGACATCAAAGAGAACTTCCATACCCTGACACCTCGATGTTGAACTTGATAATTAAGGCCACCTCTAATTGATGAACTTGTATTTGATTCATGGGAAATTTTCTTTTGGATGTGTGCATATCATGGTGCTAATTTTCTTAGTCATATGTAGTGGAGCTGGGGAAACTTTCACGGATTTTATTTAAAACATTATGTAGTATGATGTGAGCAGTTGATGTAGTTAAAAAGAACAAACTATCTGTTGAGGAAAAAGAAACAAAAATCATGGACTGAATGTCTTTTGTGAGGTTGAAAACTGTCATCATATGCATTCTACATAGAAAAACTTAAGGAATGTTGTTTTAGTTAGCAAATTTCGTGGGTTGCTGTAAGTATTTGTGTTTGAATGAATTGATAGCTTCTCTTACATCGGTCTTTCTTTTTTGGTACAGTAAGTGATGCAGTTTCAATATTAGGGGAATACTTGAAAGATGAACCTTGGTTGAAGCTTCTATGTGACCATCTCAAGTATTTAGTCCTCAACTACTCTTAACCATAGCAATTAAGATGTTGTACATTTTACAGTTCCACAAATGGACCAAGTGGCTGTATTGAGGCCAGATACTGTTGGATTTTGTTTGCAGGTTAAATTTTGCTGAAGCAACTAGAAAAGCATCCGATGCAGAAAAGCCTCCTTTAAGCATAGAGAACAATTTTGATTCTATCTCACAGGTACAATGCCCAATGACTTGTAATTGCGTCAATTTTTTGTTTCCCTTTTCGTACATGCTACCCACCAATTATGTTGCTCCGTGATTATTCGCATATGATGTGTGAATGCAACGGCCCAAGTTCACCGTTAACAAATATTGTCCTCTTTGGACTTTCCCTTTCGAGCTTCCCCTTAGGGTTTTAAAACGCGTCTTCTAAGGAGAGGTTTCCACACCCTTATAAAAATGTTCCATTCTCCTCTCCAACTAATGTGGGACCTCACAATCCACCTCTCTTAGGGGCCCAACGTCCTCACTGGCACACCATCTGGTGTCTGGCTTTGATACCATTTGTAATAACCCAAGCTCATCGCTAGCAAATATTGTGCTCTTTTCCTTTCAGGCTTCCCCTCAAAGTTTTAAAACACGTCTTCTAGAGAGAGGTTTACACACCCTTATAAAGAATGTTTCATTCCCCTCTCCAACTGTCATAGGACCTCTGAGTTTTGATGTATTCCTAATCATGATTTATGATTTAATCCAAAGATGTTTTTATAAGTTGGCAGTGCATGGATTTTAAAATATTGAAAAATGTTTTCTGATGGATCAAAATCACATTTCAATTATCTGGAAGAATGACTAAATGGTAAGAATTACTTCTTAAAACGTCTTTAGACGAAACACTTCGAGTATTTCACGTCAAAACATTACTTTAAAGAACGATATTGAACTCATATAGAGTTGATGATAAAATATGTACATTTCTTTTGTTGGCATTTCATTTATATTCTTTTCTCTTCTCAAATGGATTGTGCAAATTGTGATATGGCAGGATAAGACTAGAAGTGGCGCAAAAAATACAAGAAATGGAAAGCAAGCTAAAAAGCCGAAGGTGGAGACAGAATCACGGAACATCAAGGACATGTTTTCAAAGGCTATAAAAAGAAAAACATGA

mRNA sequence

TTTGGCGCGCGCGGTTTTTCCAGTTCTCGATTTCGTTTGGTTCCCGCCGTCGACATTCACATTTCCGTTTTCCGGCGAGGTCGGTACTTCATCTCTCTGTGGACGCCGGTGCTCTTCCGACCTTCTTCGTAGGGTTGTTCCATGGCTTGGTGGGAAGGCGTCGAGGAAACTCGTATCCTCATTGCCCCTGATCATGGGGTAAAAGAAAATGGTACTGGATGCTTGCTTTCACTTCGCCATCCAAAATCAGGAAATACGACATGTTTCTTATATGTAAACGAGTTGCTAGAGGAAATTCACTGGTTTAAGCAGTCATATTCATCGTGGTTTTTGGGAGATTATGTTACTGAAGATGGTCGTCTGTATTCCGCTACTCCAGTGGATCCTGTATTCATTTTCTTGCCCATTTTCGAGGAAGCTAGAATGAAGAAGGGAGATGATCCTGGGAAATTCAGGCAATTAGATGACATAATGTTCATCAATGGGTATCCTGGATATCTGCATTTACTGTCTGTTGCAGAGAAGTCTATGCAAGTAGTATGTGAGGTCAAAGAAATTGGATCTTCTAAGTTTTTTAGGCTTGATGATTCAAAGGTTTTGGCATGGTTATATCACAAGGTATGTCAGCTCAAAAAAACTCTATGCACACTGGATAAAAACTATGCTGCAAGGGAAGAAAAAGATACATTAAGTGATGCAGTTTCAATATTAGGGGAATACTTGAAAGATGAACCTTGGTTGAAGCTTCTATGTGACCATCTCAAGTTAAATTTTGCTGAAGCAACTAGAAAAGCATCCGATGCAGAAAAGCCTCCTTTAAGCATAGAGAACAATTTTGATTCTATCTCACAGGATAAGACTAGAAGTGGCGCAAAAAATACAAGAAATGGAAAGCAAGCTAAAAAGCCGAAGGTGGAGACAGAATCACGGAACATCAAGGACATGTTTTCAAAGGCTATAAAAAGAAAAACATGA

Coding sequence (CDS)

ATGGCTTGGTGGGAAGGCGTCGAGGAAACTCGTATCCTCATTGCCCCTGATCATGGGGTAAAAGAAAATGGTACTGGATGCTTGCTTTCACTTCGCCATCCAAAATCAGGAAATACGACATGTTTCTTATATGTAAACGAGTTGCTAGAGGAAATTCACTGGTTTAAGCAGTCATATTCATCGTGGTTTTTGGGAGATTATGTTACTGAAGATGGTCGTCTGTATTCCGCTACTCCAGTGGATCCTGTATTCATTTTCTTGCCCATTTTCGAGGAAGCTAGAATGAAGAAGGGAGATGATCCTGGGAAATTCAGGCAATTAGATGACATAATGTTCATCAATGGGTATCCTGGATATCTGCATTTACTGTCTGTTGCAGAGAAGTCTATGCAAGTAGTATGTGAGGTCAAAGAAATTGGATCTTCTAAGTTTTTTAGGCTTGATGATTCAAAGGTTTTGGCATGGTTATATCACAAGGTATGTCAGCTCAAAAAAACTCTATGCACACTGGATAAAAACTATGCTGCAAGGGAAGAAAAAGATACATTAAGTGATGCAGTTTCAATATTAGGGGAATACTTGAAAGATGAACCTTGGTTGAAGCTTCTATGTGACCATCTCAAGTTAAATTTTGCTGAAGCAACTAGAAAAGCATCCGATGCAGAAAAGCCTCCTTTAAGCATAGAGAACAATTTTGATTCTATCTCACAGGATAAGACTAGAAGTGGCGCAAAAAATACAAGAAATGGAAAGCAAGCTAAAAAGCCGAAGGTGGAGACAGAATCACGGAACATCAAGGACATGTTTTCAAAGGCTATAAAAAGAAAAACATGA

Protein sequence

MAWWEGVEETRILIAPDHGVKENGTGCLLSLRHPKSGNTTCFLYVNELLEEIHWFKQSYSSWFLGDYVTEDGRLYSATPVDPVFIFLPIFEEARMKKGDDPGKFRQLDDIMFINGYPGYLHLLSVAEKSMQVVCEVKEIGSSKFFRLDDSKVLAWLYHKVCQLKKTLCTLDKNYAAREEKDTLSDAVSILGEYLKDEPWLKLLCDHLKLNFAEATRKASDAEKPPLSIENNFDSISQDKTRSGAKNTRNGKQAKKPKVETESRNIKDMFSKAIKRKT

Homology

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BLAST of Carg08302 vs. NCBI nr
Match: KAG7029739.1 (Ribonuclease H2 subunit B [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 570.5 bits (1469), Expect = 8.1e-159
Identity = 277/277 (100.00%), Postives = 277/277 (100.00%), Query Frame = 0


Query: 1   MAWWEGVEETRILIAPDHGVKENGTGCLLSLRHPKSGNTTCFLYVNELLEEIHWFKQSYS 60
           MAWWEGVEETRILIAPDHGVKENGTGCLLSLRHPKSGNTTCFLYVNELLEEIHWFKQSYS
Sbjct: 1   MAWWEGVEETRILIAPDHGVKENGTGCLLSLRHPKSGNTTCFLYVNELLEEIHWFKQSYS 60

Query: 61  SWFLGDYVTEDGRLYSATPVDPVFIFLPIFEEARMKKGDDPGKFRQLDDIMFINGYPGYL 120
           SWFLGDYVTEDGRLYSATPVDPVFIFLPIFEEARMKKGDDPGKFRQLDDIMFINGYPGYL
Sbjct: 61  SWFLGDYVTEDGRLYSATPVDPVFIFLPIFEEARMKKGDDPGKFRQLDDIMFINGYPGYL 120

Query: 121 HLLSVAEKSMQVVCEVKEIGSSKFFRLDDSKVLAWLYHKVCQLKKTLCTLDKNYAAREEK 180
           HLLSVAEKSMQVVCEVKEIGSSKFFRLDDSKVLAWLYHKVCQLKKTLCTLDKNYAAREEK
Sbjct: 121 HLLSVAEKSMQVVCEVKEIGSSKFFRLDDSKVLAWLYHKVCQLKKTLCTLDKNYAAREEK 180

Query: 181 DTLSDAVSILGEYLKDEPWLKLLCDHLKLNFAEATRKASDAEKPPLSIENNFDSISQDKT 240
           DTLSDAVSILGEYLKDEPWLKLLCDHLKLNFAEATRKASDAEKPPLSIENNFDSISQDKT
Sbjct: 181 DTLSDAVSILGEYLKDEPWLKLLCDHLKLNFAEATRKASDAEKPPLSIENNFDSISQDKT 240

Query: 241 RSGAKNTRNGKQAKKPKVETESRNIKDMFSKAIKRKT 278
           RSGAKNTRNGKQAKKPKVETESRNIKDMFSKAIKRKT
Sbjct: 241 RSGAKNTRNGKQAKKPKVETESRNIKDMFSKAIKRKT 277

[Close]

BLAST of Carg08302 vs. NCBI nr
Match: KAG6598802.1 (Ribonuclease H2 subunit B, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 570.5 bits (1469), Expect = 8.1e-159
Identity = 277/277 (100.00%), Postives = 277/277 (100.00%), Query Frame = 0


Query: 1   MAWWEGVEETRILIAPDHGVKENGTGCLLSLRHPKSGNTTCFLYVNELLEEIHWFKQSYS 60
           MAWWEGVEETRILIAPDHGVKENGTGCLLSLRHPKSGNTTCFLYVNELLEEIHWFKQSYS
Sbjct: 22  MAWWEGVEETRILIAPDHGVKENGTGCLLSLRHPKSGNTTCFLYVNELLEEIHWFKQSYS 81

Query: 61  SWFLGDYVTEDGRLYSATPVDPVFIFLPIFEEARMKKGDDPGKFRQLDDIMFINGYPGYL 120
           SWFLGDYVTEDGRLYSATPVDPVFIFLPIFEEARMKKGDDPGKFRQLDDIMFINGYPGYL
Sbjct: 82  SWFLGDYVTEDGRLYSATPVDPVFIFLPIFEEARMKKGDDPGKFRQLDDIMFINGYPGYL 141

Query: 121 HLLSVAEKSMQVVCEVKEIGSSKFFRLDDSKVLAWLYHKVCQLKKTLCTLDKNYAAREEK 180
           HLLSVAEKSMQVVCEVKEIGSSKFFRLDDSKVLAWLYHKVCQLKKTLCTLDKNYAAREEK
Sbjct: 142 HLLSVAEKSMQVVCEVKEIGSSKFFRLDDSKVLAWLYHKVCQLKKTLCTLDKNYAAREEK 201

Query: 181 DTLSDAVSILGEYLKDEPWLKLLCDHLKLNFAEATRKASDAEKPPLSIENNFDSISQDKT 240
           DTLSDAVSILGEYLKDEPWLKLLCDHLKLNFAEATRKASDAEKPPLSIENNFDSISQDKT
Sbjct: 202 DTLSDAVSILGEYLKDEPWLKLLCDHLKLNFAEATRKASDAEKPPLSIENNFDSISQDKT 261

Query: 241 RSGAKNTRNGKQAKKPKVETESRNIKDMFSKAIKRKT 278
           RSGAKNTRNGKQAKKPKVETESRNIKDMFSKAIKRKT
Sbjct: 262 RSGAKNTRNGKQAKKPKVETESRNIKDMFSKAIKRKT 298

[Close]

BLAST of Carg08302 vs. NCBI nr
Match: XP_023546639.1 (ribonuclease H2 subunit B [Cucurbita pepo subsp. pepo])

HSP 1 Score: 570.1 bits (1468), Expect = 1.1e-158
Identity = 276/277 (99.64%), Postives = 277/277 (100.00%), Query Frame = 0


Query: 1   MAWWEGVEETRILIAPDHGVKENGTGCLLSLRHPKSGNTTCFLYVNELLEEIHWFKQSYS 60
           MAWWEGVEETRILIAPDHGVKENGTGCLLSLRHPKSGNTTCFLYVNELLEEIHWFKQSYS
Sbjct: 1   MAWWEGVEETRILIAPDHGVKENGTGCLLSLRHPKSGNTTCFLYVNELLEEIHWFKQSYS 60

Query: 61  SWFLGDYVTEDGRLYSATPVDPVFIFLPIFEEARMKKGDDPGKFRQLDDIMFINGYPGYL 120
           SWFLGDYVTEDGRLYSATPVDPVFIFLPIFEEARMKKGDDPGKFRQLDDIMFINGYPGYL
Sbjct: 61  SWFLGDYVTEDGRLYSATPVDPVFIFLPIFEEARMKKGDDPGKFRQLDDIMFINGYPGYL 120

Query: 121 HLLSVAEKSMQVVCEVKEIGSSKFFRLDDSKVLAWLYHKVCQLKKTLCTLDKNYAAREEK 180
           HLLSVAEKSMQVVCEVKE+GSSKFFRLDDSKVLAWLYHKVCQLKKTLCTLDKNYAAREEK
Sbjct: 121 HLLSVAEKSMQVVCEVKEVGSSKFFRLDDSKVLAWLYHKVCQLKKTLCTLDKNYAAREEK 180

Query: 181 DTLSDAVSILGEYLKDEPWLKLLCDHLKLNFAEATRKASDAEKPPLSIENNFDSISQDKT 240
           DTLSDAVSILGEYLKDEPWLKLLCDHLKLNFAEATRKASDAEKPPLSIENNFDSISQDKT
Sbjct: 181 DTLSDAVSILGEYLKDEPWLKLLCDHLKLNFAEATRKASDAEKPPLSIENNFDSISQDKT 240

Query: 241 RSGAKNTRNGKQAKKPKVETESRNIKDMFSKAIKRKT 278
           RSGAKNTRNGKQAKKPKVETESRNIKDMFSKAIKRKT
Sbjct: 241 RSGAKNTRNGKQAKKPKVETESRNIKDMFSKAIKRKT 277

[Close]

BLAST of Carg08302 vs. NCBI nr
Match: XP_022962545.1 (ribonuclease H2 subunit B [Cucurbita moschata])

HSP 1 Score: 567.0 bits (1460), Expect = 8.9e-158
Identity = 275/277 (99.28%), Postives = 276/277 (99.64%), Query Frame = 0


Query: 1   MAWWEGVEETRILIAPDHGVKENGTGCLLSLRHPKSGNTTCFLYVNELLEEIHWFKQSYS 60
           MAWWEGVEETRILIAPDHGVKENGTGCLLSLRHPKSGNTTCFLYVNELLEEIHWFKQSYS
Sbjct: 1   MAWWEGVEETRILIAPDHGVKENGTGCLLSLRHPKSGNTTCFLYVNELLEEIHWFKQSYS 60

Query: 61  SWFLGDYVTEDGRLYSATPVDPVFIFLPIFEEARMKKGDDPGKFRQLDDIMFINGYPGYL 120
           SWFLGDYVTEDGRLYSATPVDPVFIFLPIFEEARMKKGDDPGKFRQLDDIMFINGYP YL
Sbjct: 61  SWFLGDYVTEDGRLYSATPVDPVFIFLPIFEEARMKKGDDPGKFRQLDDIMFINGYPEYL 120

Query: 121 HLLSVAEKSMQVVCEVKEIGSSKFFRLDDSKVLAWLYHKVCQLKKTLCTLDKNYAAREEK 180
           HLLSVAEKSMQVVCEVKE+GSSKFFRLDDSKVLAWLYHKVCQLKKTLCTLDKNYAAREEK
Sbjct: 121 HLLSVAEKSMQVVCEVKEVGSSKFFRLDDSKVLAWLYHKVCQLKKTLCTLDKNYAAREEK 180

Query: 181 DTLSDAVSILGEYLKDEPWLKLLCDHLKLNFAEATRKASDAEKPPLSIENNFDSISQDKT 240
           DTLSDAVSILGEYLKDEPWLKLLCDHLKLNFAEATRKASDAEKPPLSIENNFDSISQDKT
Sbjct: 181 DTLSDAVSILGEYLKDEPWLKLLCDHLKLNFAEATRKASDAEKPPLSIENNFDSISQDKT 240

Query: 241 RSGAKNTRNGKQAKKPKVETESRNIKDMFSKAIKRKT 278
           RSGAKNTRNGKQAKKPKVETESRNIKDMFSKAIKRKT
Sbjct: 241 RSGAKNTRNGKQAKKPKVETESRNIKDMFSKAIKRKT 277

[Close]

BLAST of Carg08302 vs. NCBI nr
Match: XP_022996804.1 (ribonuclease H2 subunit B [Cucurbita maxima])

HSP 1 Score: 560.5 bits (1443), Expect = 8.3e-156
Identity = 271/277 (97.83%), Postives = 274/277 (98.92%), Query Frame = 0


Query: 1   MAWWEGVEETRILIAPDHGVKENGTGCLLSLRHPKSGNTTCFLYVNELLEEIHWFKQSYS 60
           MAWWEGVEETRILIAPDHGVKENGTGCLLSLRHPKSGNTTCFLYVNELLEEIHWFKQSYS
Sbjct: 1   MAWWEGVEETRILIAPDHGVKENGTGCLLSLRHPKSGNTTCFLYVNELLEEIHWFKQSYS 60

Query: 61  SWFLGDYVTEDGRLYSATPVDPVFIFLPIFEEARMKKGDDPGKFRQLDDIMFINGYPGYL 120
           SWFLGDYVTEDGRLYSATP+DPVFIFLPIFEEARMKKGDDPGKFRQLDDIMFINGYPGYL
Sbjct: 61  SWFLGDYVTEDGRLYSATPIDPVFIFLPIFEEARMKKGDDPGKFRQLDDIMFINGYPGYL 120

Query: 121 HLLSVAEKSMQVVCEVKEIGSSKFFRLDDSKVLAWLYHKVCQLKKTLCTLDKNYAAREEK 180
           HLLSVAEKSMQVVCEVKE+GSSKFFRLDDSKVLAWLYHKVCQLKKTLCTLDKNYAAREEK
Sbjct: 121 HLLSVAEKSMQVVCEVKEVGSSKFFRLDDSKVLAWLYHKVCQLKKTLCTLDKNYAAREEK 180

Query: 181 DTLSDAVSILGEYLKDEPWLKLLCDHLKLNFAEATRKASDAEKPPLSIENNFDSISQDKT 240
           DTLSDAVSILGEYLKDEPWLKLLCDHLKLNFAEATR+ASDAEKPPL IENNFDSISQDKT
Sbjct: 181 DTLSDAVSILGEYLKDEPWLKLLCDHLKLNFAEATRQASDAEKPPLRIENNFDSISQDKT 240

Query: 241 RSGAKNTRNGKQAKKPKVETESRNIKDMFSKAIKRKT 278
           RSGAKNTRNGKQAK  KVETESRNIKDMFSKAIKRKT
Sbjct: 241 RSGAKNTRNGKQAKNLKVETESRNIKDMFSKAIKRKT 277

[Close]

BLAST of Carg08302 vs. ExPASy Swiss-Prot
Match: Q3ZBI3 (Ribonuclease H2 subunit B OS=Bos taurus OX=9913 GN=RNASEH2B PE=2 SV=2)

HSP 1 Score: 97.4 bits (241), Expect = 2.6e-19
Identity = 66/210 (31.43%), Postives = 103/210 (49.05%), Query Frame = 0


Query: 29  LSLRHPKSGNTTCFLYVNEL--LEEIHWFKQSYSSWFLGDYVTEDGRLYSATPVDPVFIF 88
           + L +P SG  T +L+   L  L EI  FK+   SWF+ + V   G L+ ATPVDP+F+ 
Sbjct: 38  VKLANPCSGEGTIYLFNMCLPQLFEIKVFKEKNHSWFINESVQSGGLLHFATPVDPLFLL 97

Query: 89  LPIFEEARMKKGDDPGKFRQLDDIMFINGYPGYLHLLSV--AEKSMQVVCEVKEIGSSKF 148
           L    +A   K    GKF+ LD ++  + +P  + LL +   EK +Q V E KE+   K+
Sbjct: 98  LHYLIKA--DKESFQGKFQPLDQVVMDDMFPDCILLLKLPELEKLLQHVTEEKEVDKKKY 157

Query: 149 FRLDDSKVLAWLYHKVCQLKKTLCTLDKNYAAREEKDTLSDAVSILGEYLKDEPWLK--- 208
           ++    K L WL  KV Q    L T   N +AR +         +  +  KDE +++   
Sbjct: 158 YKYSKEKTLKWLGKKVNQTMAALKTNKVNVSARVQSTAFFSGGQVSSD--KDEDYVRYAH 217

Query: 209 -LLCDHLKLNFAEATRKASDAEKPPLSIEN 231
            L+ D++    ++   K     +PP S+ N
Sbjct: 218 GLISDYIPKQLSDDLSKYLKLPEPPASVRN 243

[Close]

BLAST of Carg08302 vs. ExPASy Swiss-Prot
Match: Q5HZP1 (Ribonuclease H2 subunit B OS=Xenopus laevis OX=8355 GN=rnaseh2b PE=2 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 3.8e-18
Identity = 88/301 (29.24%), Postives = 134/301 (44.52%), Query Frame = 0


Query: 6   GVEETRILIAPD--HGVKEN--GTGCLLSLRHPKSGNTTCFLYVN--ELLEEIHWFKQSY 65
           G  E  +L+AP+   G  +N       + LR P +     FL++N  + + E+  F + Y
Sbjct: 11  GQSEQWVLLAPESLSGDAKNLSDETVFVKLRAPFADKGAMFLFINSGQQICEVKAFHEEY 70

Query: 66  SSWFLGDYVTEDGRLYSATPVDPVFIFLPIFEEARMKKGDDPGKFRQLDDIMFINGYP-- 125
            SWF+G  V +DGRL  ATP+DP+F+ LP      +K   + GKF+ ++ I+    +P  
Sbjct: 71  RSWFIGQTVQQDGRLLIATPIDPLFLVLPYL----IKADKEQGKFQPVEQIVVDEEFPSC 130

Query: 126 GYLHLLSVAEKSMQVVCEVKEIGSSKFFRLDDSKVLAWLYHKVCQLKKTL-----CTLDK 185
           G L   +   KS+  V E KEIGS KF +    K L WL  KV Q  K L     C    
Sbjct: 131 GMLLQCTPVAKSLHHVTEEKEIGSKKFHKYSKEKALVWLKKKVDQTVKVLKSSKVCVGGG 190

Query: 186 NYAAR----------EEKDTLSDAVSILGEYLKDEPWLKLLCDHLKL-NFAEAT------ 245
             +A           +E+D    A  ++ EYL  E   + L  +L L + +  T      
Sbjct: 191 VQSATFIRSTQGSDVKEEDYTRYAHGLISEYL-TEDLREDLSKYLGLPDLSSPTPEPPVK 250

Query: 246 -RKASDAEKPPLSIENNFDSISQDKTRSGAKNTRNGKQAKKPKVETES-RNIKDMFSKAI 275
            R+ SDA   P+  + ++   + D     + +     Q    KV+    +NI   FS   
Sbjct: 251 KRRVSDA---PVEADEDYTKYNSDNKSRKSNSKMTAAQKSLAKVDKSGMKNISAFFSPKA 303

[Close]

BLAST of Carg08302 vs. ExPASy Swiss-Prot
Match: Q28GD9 (Ribonuclease H2 subunit B OS=Xenopus tropicalis OX=8364 GN=rnaseh2b PE=2 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 1.1e-17
Identity = 82/297 (27.61%), Postives = 125/297 (42.09%), Query Frame = 0


Query: 6   GVEETRILIAP----DHGVKENGTGCLLSLRHPKSGNTTCFLYVN--ELLEEIHWFKQSY 65
           G  E  +L+AP    D     +       LR P +   + FL+++  + + E+  F + Y
Sbjct: 11  GQSEQWVLLAPESLSDDAKNLSDKTIFAKLRAPCTDKGSMFLFIDSGQQICEVKAFHEEY 70

Query: 66  SSWFLGDYVTEDGRLYSATPVDPVFIFLPIFEEARMKKGDDPGKFRQLDDIMFINGYPGY 125
            SWF+G  V +DGRL  ATP+DP+F+ L       +K   + GKF+ ++ I+    +P  
Sbjct: 71  RSWFIGQTVQQDGRLLIATPIDPMFLVLHYL----IKADKEQGKFQPVEQIVVDEEFPSC 130

Query: 126 LHLL--SVAEKSMQVVCEVKEIGSSKFFRLDDSKVLAWLYHKVCQLKKTL---------- 185
             LL  +   KS+  V E KEIGS KF++    K L WL  KV    K L          
Sbjct: 131 SMLLQCTPVSKSLHHVTEEKEIGSKKFYKYSKEKTLVWLKKKVELTVKVLKSSNICVGGG 190

Query: 186 ---CTLDKNYAARE--EKDTLSDAVSILGEYLKD---EPWLKLL-CDHLKLNFAEATRKA 245
               T  +N    +  E+D    A  ++ EYL +   E   K L    L     E   K 
Sbjct: 191 VQSATFIRNTQGSDVKEEDYTRYAHGLISEYLTEDLREDLSKYLGLPDLSSPTPEPPVKK 250

Query: 246 SDAEKPPLSIENNFDSISQDKTRSGAKNTRNGKQAKKPKVETES-RNIKDMFSKAIK 275
               + P+  E ++   + D     + +     Q    KV+    +NI   FS   K
Sbjct: 251 RKVSEAPVEAEEDYTKFNSDSKNKKSNSKMTAAQKSLAKVDKSGMKNISAFFSPKAK 303

[Close]

BLAST of Carg08302 vs. ExPASy Swiss-Prot
Match: Q5XI96 (Ribonuclease H2 subunit B OS=Rattus norvegicus OX=10116 GN=Rnaseh2b PE=2 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 1.6e-16
Identity = 75/275 (27.27%), Postives = 120/275 (43.64%), Query Frame = 0


Query: 29  LSLRHPKSGNTTCFLY--VNELLEEIHWFKQSYSSWFLGDYVTEDGRLYSATPVDPVFIF 88
           + L +P SG    +L     + L EI  FK+ + SWF+   V   G L+ ATP+DP+F+ 
Sbjct: 37  VKLVNPHSGEGATYLIDACLQKLFEIKVFKEKHHSWFINQSVQSGGLLHFATPMDPLFLL 96

Query: 89  LPIFEEARMKKGDDPGKFRQLDDIMFINGYPGYLHLLSV--AEKSMQVVCEVKEIGSSKF 148
           L       + K    GK++ LD ++  + +P    LL     EKS++ V E KE+ S K+
Sbjct: 97  LHY-----LIKAGKEGKYQPLDQVVVDDKFPDCTLLLRFPELEKSLRHVTEEKEVNSKKY 156

Query: 149 FRLDDSKVLAWLYHKVCQLKKTLCTLDKNYAAREEKDTLSDAVSILGEYLKDEPWLK--- 208
           ++    K L WL  KV Q    L     N  AR +       V +  +  K+E +++   
Sbjct: 157 YKYSTEKTLKWLEKKVNQTVAALKANHVNVGARVQSSAYFSGVQVSRD--KEEDYVRYAH 216

Query: 209 -LLCDHLKLNFAEATRKASDAEKPPLSIEN----------NFDSISQDKTRSGAKNTRNG 268
            L+ D++    ++   K     +PP S+ N                +D T+   K+ +NG
Sbjct: 217 GLISDYIPKELSDDLSKLLKLPEPPASLPNPPAKKLKLADEPVEAKEDYTKFNTKDLKNG 276

Query: 269 KQAKK--------PKVETESRNIKDMFSKAIKRKT 278
           K+  K         KV+       D F  A  +KT
Sbjct: 277 KKNSKMTAAQKALAKVDKSGMKSIDAFFGAKNKKT 304

[Close]

BLAST of Carg08302 vs. ExPASy Swiss-Prot
Match: Q80ZV0 (Ribonuclease H2 subunit B OS=Mus musculus OX=10090 GN=Rnaseh2b PE=1 SV=2)

HSP 1 Score: 87.0 bits (214), Expect = 3.5e-16
Identity = 76/297 (25.59%), Postives = 127/297 (42.76%), Query Frame = 0


Query: 12  ILIAPDH-----GVKENGTGCLLSLRHPKSGNTTCFL--YVNELLEEIHWFKQSYSSWFL 71
           + + P+H       K+  +   + L +P SG    +L     + L EI  FK+ + SWF+
Sbjct: 16  VFLLPEHLKDASKKKKKSSLLFVKLANPHSGEGATYLIDMCLQQLFEIKVFKEKHHSWFI 75

Query: 72  GDYVTEDGRLYSATPVDPVFIFLPIFEEARMKKGDDPGKFRQLDDIMFINGYPGYLHLLS 131
              V   G L+ ATP+DP+F+ L       + K    GK++ LD ++  + +P    LL 
Sbjct: 76  NQSVQSGGLLHFATPMDPLFLLLHY-----LLKAGKEGKYQPLDQVVVDDTFPDCTLLLR 135

Query: 132 V--AEKSMQVVCEVKEIGSSKFFRLDDSKVLAWLYHKVCQLKKTLCTLDKNYAAREEKDT 191
               EKS++ V E KE+ S K+++    K L WL  KV Q    L   + N  AR +   
Sbjct: 136 FPELEKSLRHVTEEKEVNSKKYYKYSSEKTLKWLEKKVNQTVVALKANNVNVGARVQSSA 195

Query: 192 LSDAVSILGEYLKDEPWLK----LLCDHLKLNFAEATRKASDAEKPPLSIENNFD----- 251
                 +  +  K+E +++    L+ D++    ++   K     +PP S+ N        
Sbjct: 196 YFSGGQVSRD--KEEDYVRYAHGLISDYIPKELSDDLSKFLKLPEPPASLTNPPSKKLKL 255

Query: 252 -----SISQDKTRSGAKNTRNGKQAKK--------PKVETESRNIKDMFSKAIKRKT 278
                   +D T+   K+ + GK+  K         KV+       D F  A  +KT
Sbjct: 256 SDEPVEAKEDYTKFNTKDLKTGKKNSKMTAAQKALAKVDKSGMKSIDAFFGAKNKKT 305

[Close]

BLAST of Carg08302 vs. ExPASy TrEMBL
Match: A0A6J1HFF3 (Ribonuclease H2 subunit B OS=Cucurbita moschata OX=3662 GN=LOC111462943 PE=4 SV=1)

HSP 1 Score: 567.0 bits (1460), Expect = 4.3e-158
Identity = 275/277 (99.28%), Postives = 276/277 (99.64%), Query Frame = 0


Query: 1   MAWWEGVEETRILIAPDHGVKENGTGCLLSLRHPKSGNTTCFLYVNELLEEIHWFKQSYS 60
           MAWWEGVEETRILIAPDHGVKENGTGCLLSLRHPKSGNTTCFLYVNELLEEIHWFKQSYS
Sbjct: 1   MAWWEGVEETRILIAPDHGVKENGTGCLLSLRHPKSGNTTCFLYVNELLEEIHWFKQSYS 60

Query: 61  SWFLGDYVTEDGRLYSATPVDPVFIFLPIFEEARMKKGDDPGKFRQLDDIMFINGYPGYL 120
           SWFLGDYVTEDGRLYSATPVDPVFIFLPIFEEARMKKGDDPGKFRQLDDIMFINGYP YL
Sbjct: 61  SWFLGDYVTEDGRLYSATPVDPVFIFLPIFEEARMKKGDDPGKFRQLDDIMFINGYPEYL 120

Query: 121 HLLSVAEKSMQVVCEVKEIGSSKFFRLDDSKVLAWLYHKVCQLKKTLCTLDKNYAAREEK 180
           HLLSVAEKSMQVVCEVKE+GSSKFFRLDDSKVLAWLYHKVCQLKKTLCTLDKNYAAREEK
Sbjct: 121 HLLSVAEKSMQVVCEVKEVGSSKFFRLDDSKVLAWLYHKVCQLKKTLCTLDKNYAAREEK 180

Query: 181 DTLSDAVSILGEYLKDEPWLKLLCDHLKLNFAEATRKASDAEKPPLSIENNFDSISQDKT 240
           DTLSDAVSILGEYLKDEPWLKLLCDHLKLNFAEATRKASDAEKPPLSIENNFDSISQDKT
Sbjct: 181 DTLSDAVSILGEYLKDEPWLKLLCDHLKLNFAEATRKASDAEKPPLSIENNFDSISQDKT 240

Query: 241 RSGAKNTRNGKQAKKPKVETESRNIKDMFSKAIKRKT 278
           RSGAKNTRNGKQAKKPKVETESRNIKDMFSKAIKRKT
Sbjct: 241 RSGAKNTRNGKQAKKPKVETESRNIKDMFSKAIKRKT 277

[Close]

BLAST of Carg08302 vs. ExPASy TrEMBL
Match: A0A6J1K9Q1 (Ribonuclease H2 subunit B OS=Cucurbita maxima OX=3661 GN=LOC111491930 PE=4 SV=1)

HSP 1 Score: 560.5 bits (1443), Expect = 4.0e-156
Identity = 271/277 (97.83%), Postives = 274/277 (98.92%), Query Frame = 0


Query: 1   MAWWEGVEETRILIAPDHGVKENGTGCLLSLRHPKSGNTTCFLYVNELLEEIHWFKQSYS 60
           MAWWEGVEETRILIAPDHGVKENGTGCLLSLRHPKSGNTTCFLYVNELLEEIHWFKQSYS
Sbjct: 1   MAWWEGVEETRILIAPDHGVKENGTGCLLSLRHPKSGNTTCFLYVNELLEEIHWFKQSYS 60

Query: 61  SWFLGDYVTEDGRLYSATPVDPVFIFLPIFEEARMKKGDDPGKFRQLDDIMFINGYPGYL 120
           SWFLGDYVTEDGRLYSATP+DPVFIFLPIFEEARMKKGDDPGKFRQLDDIMFINGYPGYL
Sbjct: 61  SWFLGDYVTEDGRLYSATPIDPVFIFLPIFEEARMKKGDDPGKFRQLDDIMFINGYPGYL 120

Query: 121 HLLSVAEKSMQVVCEVKEIGSSKFFRLDDSKVLAWLYHKVCQLKKTLCTLDKNYAAREEK 180
           HLLSVAEKSMQVVCEVKE+GSSKFFRLDDSKVLAWLYHKVCQLKKTLCTLDKNYAAREEK
Sbjct: 121 HLLSVAEKSMQVVCEVKEVGSSKFFRLDDSKVLAWLYHKVCQLKKTLCTLDKNYAAREEK 180

Query: 181 DTLSDAVSILGEYLKDEPWLKLLCDHLKLNFAEATRKASDAEKPPLSIENNFDSISQDKT 240
           DTLSDAVSILGEYLKDEPWLKLLCDHLKLNFAEATR+ASDAEKPPL IENNFDSISQDKT
Sbjct: 181 DTLSDAVSILGEYLKDEPWLKLLCDHLKLNFAEATRQASDAEKPPLRIENNFDSISQDKT 240

Query: 241 RSGAKNTRNGKQAKKPKVETESRNIKDMFSKAIKRKT 278
           RSGAKNTRNGKQAK  KVETESRNIKDMFSKAIKRKT
Sbjct: 241 RSGAKNTRNGKQAKNLKVETESRNIKDMFSKAIKRKT 277

[Close]

BLAST of Carg08302 vs. ExPASy TrEMBL
Match: A0A1S3BBT5 (Ribonuclease H2 subunit B OS=Cucumis melo OX=3656 GN=LOC103488218 PE=4 SV=1)

HSP 1 Score: 531.6 bits (1368), Expect = 2.0e-147
Identity = 257/276 (93.12%), Postives = 266/276 (96.38%), Query Frame = 0


Query: 1   MAWWEGVEETRILIAPDHGVKENGTGCLLSLRHPKSGNTTCFLYVNELLEEIHWFKQSYS 60
           MAW+EGVEE RILIAPDHGVKE GTGC LSLRHPKSGN TCFLYVNE+LEEIHWFKQSYS
Sbjct: 53  MAWYEGVEEKRILIAPDHGVKEIGTGCFLSLRHPKSGNPTCFLYVNEMLEEIHWFKQSYS 112

Query: 61  SWFLGDYVTEDGRLYSATPVDPVFIFLPIFEEARMKKGDDPGKFRQLDDIMFINGYPGYL 120
           SWFLGDYVTEDGRLYSATPVDPVFIFLPIFEEARMKKGDDPGKFRQLDDIMFINGYPGYL
Sbjct: 113 SWFLGDYVTEDGRLYSATPVDPVFIFLPIFEEARMKKGDDPGKFRQLDDIMFINGYPGYL 172

Query: 121 HLLSVAEKSMQVVCEVKEIGSSKFFRLDDSKVLAWLYHKVCQLKKTLCTLDKNYAAREEK 180
           HLLS+AEKSMQVVCEVKE+GSS+FFRLDDSKVLAWLYHKVCQLKKTLCTLDKNYAAREEK
Sbjct: 173 HLLSLAEKSMQVVCEVKEVGSSRFFRLDDSKVLAWLYHKVCQLKKTLCTLDKNYAAREEK 232

Query: 181 DTLSDAVSILGEYLKDEPWLKLLCDHLKLNFAEATRKASDAEKPPLSIENNFDSISQDKT 240
           +TLSDAVSILGEYLKD+PWLKLLCDHLKLNFAEATRKASD+EK P SIEN FDSISQDK+
Sbjct: 233 ETLSDAVSILGEYLKDQPWLKLLCDHLKLNFAEATRKASDSEKAPTSIENKFDSISQDKS 292

Query: 241 RSGAKNTRNGKQAKKPKVETESRNIKDMFSKAIKRK 277
            S AKNTRNGKQAKKPKVETESRNIKDMFSKA KRK
Sbjct: 293 WSDAKNTRNGKQAKKPKVETESRNIKDMFSKAAKRK 328

[Close]

BLAST of Carg08302 vs. ExPASy TrEMBL
Match: A0A1S3BCJ9 (Ribonuclease H2 subunit B OS=Cucumis melo OX=3656 GN=LOC103488218 PE=4 SV=1)

HSP 1 Score: 531.6 bits (1368), Expect = 2.0e-147
Identity = 257/276 (93.12%), Postives = 266/276 (96.38%), Query Frame = 0


Query: 1   MAWWEGVEETRILIAPDHGVKENGTGCLLSLRHPKSGNTTCFLYVNELLEEIHWFKQSYS 60
           MAW+EGVEE RILIAPDHGVKE GTGC LSLRHPKSGN TCFLYVNE+LEEIHWFKQSYS
Sbjct: 1   MAWYEGVEEKRILIAPDHGVKEIGTGCFLSLRHPKSGNPTCFLYVNEMLEEIHWFKQSYS 60

Query: 61  SWFLGDYVTEDGRLYSATPVDPVFIFLPIFEEARMKKGDDPGKFRQLDDIMFINGYPGYL 120
           SWFLGDYVTEDGRLYSATPVDPVFIFLPIFEEARMKKGDDPGKFRQLDDIMFINGYPGYL
Sbjct: 61  SWFLGDYVTEDGRLYSATPVDPVFIFLPIFEEARMKKGDDPGKFRQLDDIMFINGYPGYL 120

Query: 121 HLLSVAEKSMQVVCEVKEIGSSKFFRLDDSKVLAWLYHKVCQLKKTLCTLDKNYAAREEK 180
           HLLS+AEKSMQVVCEVKE+GSS+FFRLDDSKVLAWLYHKVCQLKKTLCTLDKNYAAREEK
Sbjct: 121 HLLSLAEKSMQVVCEVKEVGSSRFFRLDDSKVLAWLYHKVCQLKKTLCTLDKNYAAREEK 180

Query: 181 DTLSDAVSILGEYLKDEPWLKLLCDHLKLNFAEATRKASDAEKPPLSIENNFDSISQDKT 240
           +TLSDAVSILGEYLKD+PWLKLLCDHLKLNFAEATRKASD+EK P SIEN FDSISQDK+
Sbjct: 181 ETLSDAVSILGEYLKDQPWLKLLCDHLKLNFAEATRKASDSEKAPTSIENKFDSISQDKS 240

Query: 241 RSGAKNTRNGKQAKKPKVETESRNIKDMFSKAIKRK 277
            S AKNTRNGKQAKKPKVETESRNIKDMFSKA KRK
Sbjct: 241 WSDAKNTRNGKQAKKPKVETESRNIKDMFSKAAKRK 276

[Close]

BLAST of Carg08302 vs. ExPASy TrEMBL
Match: A0A6J1BTT6 (Ribonuclease H2 subunit B OS=Momordica charantia OX=3673 GN=LOC111004733 PE=4 SV=1)

HSP 1 Score: 525.4 bits (1352), Expect = 1.4e-145
Identity = 255/277 (92.06%), Postives = 266/277 (96.03%), Query Frame = 0


Query: 1   MAWWEGVEETRILIAPDHGVKENGTGCLLSLRHPKSGNTTCFLYVNELLEEIHWFKQSYS 60
           MAWWEGV+ETRILIAPD GVK N +GCLLSLRHPKSGNTTCFLY NE+L+EIHWFKQSYS
Sbjct: 1   MAWWEGVDETRILIAPDQGVKGNDSGCLLSLRHPKSGNTTCFLYGNEMLKEIHWFKQSYS 60

Query: 61  SWFLGDYVTEDGRLYSATPVDPVFIFLPIFEEARMKKGDDPGKFRQLDDIMFINGYPGYL 120
           SWFLGDY+TEDGRLYSATPVDPVFIFLPIFEEARMKK DDPGKFRQLDDIMFINGYPGY 
Sbjct: 61  SWFLGDYITEDGRLYSATPVDPVFIFLPIFEEARMKKRDDPGKFRQLDDIMFINGYPGYQ 120

Query: 121 HLLSVAEKSMQVVCEVKEIGSSKFFRLDDSKVLAWLYHKVCQLKKTLCTLDKNYAAREEK 180
            LLSVAEKSMQVVCEVKE+GSS+FFRLDDSKVLAWLYHKVCQLKKTLCTLDKNYAAREEK
Sbjct: 121 RLLSVAEKSMQVVCEVKEVGSSRFFRLDDSKVLAWLYHKVCQLKKTLCTLDKNYAAREEK 180

Query: 181 DTLSDAVSILGEYLKDEPWLKLLCDHLKLNFAEATRKASDAEKPPLSIENNFDS-ISQDK 240
           DTLSDAVSILGEYLKDEPWLKLLCDHLKLNFAEA RKASDAEK P+SIENNFDS ISQDK
Sbjct: 181 DTLSDAVSILGEYLKDEPWLKLLCDHLKLNFAEAIRKASDAEKAPISIENNFDSNISQDK 240

Query: 241 TRSGAKNTRNGKQAKKPKVETESRNIKDMFSKAIKRK 277
           +RSGAKNTRNGKQ+KKPKVET+SRNIKDMFSKA KRK
Sbjct: 241 SRSGAKNTRNGKQSKKPKVETQSRNIKDMFSKATKRK 277

[Close]

BLAST of Carg08302 vs. TAIR 10
Match: AT4G20325.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: nucleus; CONTAINS InterPro DOMAIN/s: Ribonuclease H2, subunit B (InterPro:IPR019024); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 357.5 bits (916), Expect = 1.0e-98
Identity = 172/274 (62.77%), Postives = 215/274 (78.47%), Query Frame = 0


Query: 3   WWEGVEETRILIAPDHGVKENGTGCLLSLRHPKSGNTTCFLYVNELLEEIHWFKQSYSSW 62
           WWEG E+TR+LIAPD G   N  G LL+LRHPKS N TCFL+ NE+L+E+ WFKQSY SW
Sbjct: 6   WWEGAEKTRVLIAPDSGCGGNKPGELLTLRHPKSENGTCFLFNNEMLQELQWFKQSYGSW 65

Query: 63  FLGDYVTEDGRLYSATPVDPVFIFLPIFEEARMKKGDDPGKFRQLDDIMFINGYPGYLHL 122
           FLGDY++EDG LY ATPVDPVFI LPIF+EARMKKG++ GKFRQLD+I+F+ GYPGY HL
Sbjct: 66  FLGDYISEDGSLYMATPVDPVFILLPIFDEARMKKGENSGKFRQLDEILFVEGYPGYQHL 125

Query: 123 LSVAEKSMQVVCEVKEIGSSKFFRLDDSKVLAWLYHKVCQLKKTLCTLDKNYAAREEKDT 182
           LS+AEK M++VC+ +E+GS KF+RLD+SKVLAWL  K+  LK +L  LDKNYAA++EK T
Sbjct: 126 LSLAEKCMEIVCQTQEVGSMKFYRLDNSKVLAWLSCKIYCLKNSLPELDKNYAAQDEKQT 185

Query: 183 LSDAVSILGEYLKDEPWLKLLCDHLKLNFAEATRKASDAEKPPLSIENNFDSISQDKTRS 242
           L D+VSI+GEYLK EPWLKLL DHL L F + T K ++ E  P + ENN  S +  + ++
Sbjct: 186 LVDSVSIVGEYLKTEPWLKLLYDHLGLKFVDPTMKETNMENLPTANENNMASSNSIQEKA 245

Query: 243 GAKNTRNGKQAKKPKVETESRNIKDMFSKAIKRK 277
              N + GKQ K+ KVET S+NI+DMFS+A K+K
Sbjct: 246 ---NKKPGKQTKQAKVETGSKNIRDMFSRACKKK 276

[Close]

BLAST of Carg08302 vs. TAIR 10
Match: AT4G20325.2 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: nucleus; CONTAINS InterPro DOMAIN/s: Ribonuclease H2, subunit B (InterPro:IPR019024); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 251.5 bits (641), Expect = 7.7e-67
Identity = 109/154 (70.78%), Postives = 132/154 (85.71%), Query Frame = 0


Query: 3   WWEGVEETRILIAPDHGVKENGTGCLLSLRHPKSGNTTCFLYVNELLEEIHWFKQSYSSW 62
           WWEG E+TR+LIAPD G   N  G LL+LRHPKS N TCFL+ NE+L+E+ WFKQSY SW
Sbjct: 6   WWEGAEKTRVLIAPDSGCGGNKPGELLTLRHPKSENGTCFLFNNEMLQELQWFKQSYGSW 65

Query: 63  FLGDYVTEDGRLYSATPVDPVFIFLPIFEEARMKKGDDPGKFRQLDDIMFINGYPGYLHL 122
           FLGDY++EDG LY ATPVDPVFI LPIF+EARMKKG++ GKFRQLD+I+F+ GYPGY HL
Sbjct: 66  FLGDYISEDGSLYMATPVDPVFILLPIFDEARMKKGENSGKFRQLDEILFVEGYPGYQHL 125

Query: 123 LSVAEKSMQVVCEVKEIGSSKFFRLDDSKVLAWL 157
           LS+AEK M++VC+ +E+GS KF+RLD+SKVLAWL
Sbjct: 126 LSLAEKCMEIVCQTQEVGSMKFYRLDNSKVLAWL 159

The following BLAST results are available for this feature:

BLAST of Carg08302 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7029739.1	8.1e-159	100.00	Ribonuclease H2 subunit B [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6598802.1	8.1e-159	100.00	Ribonuclease H2 subunit B, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023546639.1	1.1e-158	99.64	ribonuclease H2 subunit B [Cucurbita pepo subsp. pepo]	[more]
XP_022962545.1	8.9e-158	99.28	ribonuclease H2 subunit B [Cucurbita moschata]	[more]
XP_022996804.1	8.3e-156	97.83	ribonuclease H2 subunit B [Cucurbita maxima]	[more]

BLAST of Carg08302 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q3ZBI3	2.6e-19	31.43	Ribonuclease H2 subunit B OS=Bos taurus OX=9913 GN=RNASEH2B PE=2 SV=2	[more]
Q5HZP1	3.8e-18	29.24	Ribonuclease H2 subunit B OS=Xenopus laevis OX=8355 GN=rnaseh2b PE=2 SV=1	[more]
Q28GD9	1.1e-17	27.61	Ribonuclease H2 subunit B OS=Xenopus tropicalis OX=8364 GN=rnaseh2b PE=2 SV=1	[more]
Q5XI96	1.6e-16	27.27	Ribonuclease H2 subunit B OS=Rattus norvegicus OX=10116 GN=Rnaseh2b PE=2 SV=1	[more]
Q80ZV0	3.5e-16	25.59	Ribonuclease H2 subunit B OS=Mus musculus OX=10090 GN=Rnaseh2b PE=1 SV=2	[more]

BLAST of Carg08302 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HFF3	4.3e-158	99.28	Ribonuclease H2 subunit B OS=Cucurbita moschata OX=3662 GN=LOC111462943 PE=4 SV=...	[more]
A0A6J1K9Q1	4.0e-156	97.83	Ribonuclease H2 subunit B OS=Cucurbita maxima OX=3661 GN=LOC111491930 PE=4 SV=1	[more]
A0A1S3BBT5	2.0e-147	93.12	Ribonuclease H2 subunit B OS=Cucumis melo OX=3656 GN=LOC103488218 PE=4 SV=1	[more]
A0A1S3BCJ9	2.0e-147	93.12	Ribonuclease H2 subunit B OS=Cucumis melo OX=3656 GN=LOC103488218 PE=4 SV=1	[more]
A0A6J1BTT6	1.4e-145	92.06	Ribonuclease H2 subunit B OS=Momordica charantia OX=3673 GN=LOC111004733 PE=4 SV...	[more]

BLAST of Carg08302 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G20325.1	1.0e-98	62.77	FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow...	[more]
AT4G20325.2	7.7e-67	70.78	FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR019024	Ribonuclease H2 subunit B, wHTH domain	PFAM	PF09468	RNase_H2-Ydr279	coord: 84..170 e-value: 1.3E-10 score: 41.7
None	No IPR available	GENE3D	1.10.20.120		coord: 80..208 e-value: 3.2E-28 score: 100.5
None	No IPR available	GENE3D	2.20.25.530		coord: 8..79 e-value: 1.3E-15 score: 59.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 229..249
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 220..277
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 252..271
IPR041195	Rnh202, triple barrel domain	PFAM	PF17745	Ydr279_N	coord: 28..81 e-value: 1.2E-6 score: 28.6
IPR040456	Ribonuclease H2 subunit B	PANTHER	PTHR13383	RIBONUCLEASE H2 SUBUNIT B	coord: 1..275
IPR040456	Ribonuclease H2 subunit B	CDD	cd09270	RNase_H2-B	coord: 12..185 e-value: 1.39714E-44 score: 148.221

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg08302-RA	Carg08302-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0000455	enzyme-directed rRNA pseudouridine synthesis
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005634	nucleus
cellular_component	GO:0032299	ribonuclease H2 complex
molecular_function	GO:0009982	pseudouridine synthase activity
molecular_function	GO:0003723	RNA binding