Carg08294 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg08294
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Protein kinase domain-containing protein
Location	Carg_Chr05: 3237903 .. 3241905 (+)
RNA-Seq Expression	Carg08294
Synteny	Carg08294

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TGGTGATTCTCAGGGATTTATCTGTTGATTATTTATTTGTACATTCGTAATCAGTATTGGGATAGAGTTTAGAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTGATTAATGAACGTCAGCCATGGAAGCTGAGAGAATGGAGAAAGAACAACAGCGGACCACCAGGAAAAGGCCTCGTTCCGCATGGGATGTGGTGCCATCCGAACAAGAGGTTAGTTCGTTGGCTTTGGGATTGGATTTTCAGCGATGACGACGTTGAATTCCGAAGGTCACGGCAATTAGGGCTTTTATTTCGAATCATGAAGGTTGCTGGGTGCGTCTTTTTCCTGTGTAGGCTAATAAGGCTTTGGCGGTGGTTAAGGATGAGGTCAAAAGGCATGCCTCGCCCCCGAGAAGAGACGATGATCGTGAAGGACACTATGTCTTCAACCTCGGCGAGAATCTCACTCCGAGATGTGAGTGCTTTTTTATTGTTATTGTCGGTTTGCCTTCTAAATTGGATGCTGAATATACTATGATAGTCTGGTATGGAGTGTGTGAAATCGAGTCTGCAATGTTGGGGTTTTTTCCTTTATCAGACGAGGGATTTACGGTAAATTCTTAATACAAGTAAGGCAGTATTTTGAGGCACACATCACGAGAACCATCATCTTATGTTGTTTGCTTGAAAGAAAGAAAACACATGAGGGAAAGAAAGAATTCTTGGAACTTTCTGTGAGATTTTTTTGGTTGTTGCCACCTGGTGTAAGCTGCAATGCCATTCTTTGACTATATCATTAATTCTTTTGTAGTATAGACCCTTTTGTAACTTCCTGCAAAGGTTGAGCTGGAGACTCTACTTGTGAATTTCATATTATCAATGAAATTACCTTTTATCAGGGAAAGAAATCACATTAATATTTTAAGGTTTCCCCGCAGCTGCGGAATGAACATCCCTTATTTGTTTTAAATACCCAAAACCATATATATATATATATAGTCTTATGTCCTCTTTCGTTTTTGTTATTTACTCAAGTTATCCATATCTTAAATAGCAATTAGGTTTTCGATTGGTCATTATCTGAATTGTTTTGGTCTTTTGATTTGTTATTCTTGAATCTTACTGGGGTAATCGGTAACATGAACGTCTTGCAGATAAAATCCTCAGCAAGATGGGTGAAGGTTAGCTGTCGTGTTCCCTGCTGTCTGCAAGATTTTATGTTTTTGTCATTCTGCTGGAGTCCACTGAGCAAATTTCTAGTGCTGTAGGCACATTTGGTCGAGTTTTGGAATGCTGGGACCGGCAAACTCGAGAATATGTAGCCATTAAGGTAGTTCGGAGCATAAGAAAGTACCGTGATGCAGCCATGGTTGAGGTGGATATACTTAAGCATCTTGCTCAGAATGACACAGGCTGCTTACGGTGTGTATCTATTTCCTTGTTTTATAGTCTTCAATTCATCAGATAACACAATCTAACTCTGGTTTGCAACATATTTCTCATGCAGTTGTGTGCAGATTAGAAATTGGTTTGACTACCGCAATCACATATGTATAGTAAGTATTTCAATTAAAGCCCATTGATTTATCTCTTTTTTCTCCCTTTCTTGATATTTAAGATGTTATTGCGATCCCTTTGATGTTGTATTTTCTGGTAACTGAAGTTAATCTCTTTCAGTTATCATAATAGTGGCGGTTTTTCTTTTGCTATTGGGTAAACCTTATCAATTATTGTGTACATGCATTGTTCACATATACATGAGTTCTTTCGTACATGTGGACTTATTCATAGAATTGGAAAATTAACAAAACCCTTCCTTTGAATGCATTTGAATGGTTTGATCAGGTATTTGAGAGGCTTGGACCAAGCTTATTTGATTTTCTAAAGAGAAATAAATACCACCCATTCCCTGTGGATCTTGTTCGGGAGTTTGGACGACAGCTTTTGGAATCTGTAGCATGTGTGTGGATTTATTTTCAACCTCAGCATTCTGATTTTCATTTATCTCTTTTTCCCTAAAGAACTCAGAAACCATTTTCATTGTTATTTCCTCTGTTCAGGGGTGGAAAATGTCTAATCATACCATGTTTAACTTGTTTACTTTGCTTCATCATATCTGGCAGATATGCATGATTTACACTTAATCCACACAGACCTGAAGCCAGAAAATATACTTCTTGTGTCTTCTGAATATATAAAGCTTCCTGGTTGTAAGGTATTTAAATATTTCGAATCTGAAAATTTGGGTGAAAACTCAAATGTATCTTAAAAACGATGCTTGAAATGATACAGAGGGTTTCGTCGGATGAAACGCAATTCAGGTGCTTGCCCAAGTCTAGTGCAATTAAGCTGATTGATTTTGGTAGTACTGCATTTGATAATGAGAATCATAGCTCCATTGTTTCTACGAGGCATTACAGAGCCCCTGAGGTTATTCTAGGTAACTAGTTATGTGGTTGGCTAGTGAAATTTGTGTTTGTGTATAGTTTTCTCCCCTTTCCTTCTCTGATTCCTAATTGAGAAACCTTCTGTGTTGAAACTTTTCCCAAAATTTCATTTTATTTATGTTGTTGCATAGAATTAAATAGTATCTGGAGGAAGGGGAAAGATGAACCCCAGTGGTAGCATTACGGAAGAAGGCGGAATCTGTCGAAACACCTTTAAAAATGTGATATATTTACGTTTTTTGCTTCTTTCTTGCTAAAATATTGTATATCTGTCTGGTCACTTTTACCCATAAGGCAATAACTCTTGTCATTGATGGTAGTTCGTGGCTCTTGTCGTCCAACGGTTCATACATATGTATGCAGCAGTGTAGAAATGTTTTAAGCTTTTCATTAGTTTCTAAATCTCATTGGGAGCGTGAGTGGAAGGTGTTTATTCTATGATGGAGGCTCCAATTTTTTAACATTTGGGTGTCAAAATTGTTTTGGGGTTAGAGTGACATTCATGGTTGTCTCTTCAGCATCCGTTTTTGCTGCTTGGCCCTGAGTTACACATTTCCTACTCATGGGCCCCTGCCTGAAAATTTATCATCAGATGGACATGAAAGTAATATAATTCATTGCATATCCCAGGATACTATTATATGATATTTGATTATTGCACCAAGAATCTCAGCAAGGACATGTTGACAATGTTAGATATTGCGTCACAAATGCCTTACATTATATACATGCTGTAGGCTCACCTGCATTGATGAATGTTATGCAGGTCTTGGATGGAGTTATCCATGCGATTTGTGGAGTATCGGTTGCATTCTTGTCGAACTGTGTTCGGTTAGTATTTGTTGAACCTGACTTAGGTTTGATTAGGTTGAAGTTTATATAAAAAGTTGTTAATTACTGGAAGCCTGTAGGGCAAAGCACTGTTTCAAACTCACGAGAACTTGGAGCATTTGGCAATGATGGAGAGGGTGTTGGGACCTATACCAGGGCACATGATTCAGAGTGCTGAGTACTGAAACATCCCGATTTCTCTTCATTTATTTCCCTTGTAGAGTAACAAATGGACTAATTTTCGATCCGTTTCATGTAGCCAAAACGCAGAAAAATATTTCAAACGAGGTTTGCGTCTAAACTGGCCCGAAGGAGCAGTTTCAAGAGAAAGCATTCGAGCCGTGAAGAAACTCGATCGTTTGAAGGTCTGTTAATTAGTATGTTTCTGTGGTTCTATAGAAGATGATGGTAAGCTCATATCAAATTGTTCTCTTTGTTTTCAGGATATGGTTTCACAACATGTGGGTTTCTCCAGGCTATTGACTGATCTGTTGTATGATCTATTGAAATACGACCCTTCGGAACGCCCAACAGCTCGACAAGCCCTTAACCATTCTTTCTTTAAGAGTATAGCATGAGAAGGTACGATTCCATGCTTAGTTACAGTTGCAATACAAGTCTGTCTGCAAAATGCTGGCTTTTTTATTTCAGGGGAAAGAGAGAGAGAGATGATTAAGAATGCTATGCAGACAATGATTTTTACTTGTATTTGTAGAAGTATGATTATTAATGAAAAGCATGTCA

mRNA sequence

TGGTGATTCTCAGGGATTTATCTGTTGATTATTTATTTGTACATTCGTAATCAGTATTGGGATAGAGTTTAGAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTGATTAATGAACGTCAGCCATGGAAGCTGAGAGAATGGAGAAAGAACAACAGCGGACCACCAGGAAAAGGCCTCGTTCCGCATGGGATGTGGTGCCATCCGAACAAGAGGCTAATAAGGCTTTGGCGGTGGTTAAGGATGAGGTCAAAAGGCATGCCTCGCCCCCGAGAAGAGACGATGATCGTGAAGGACACTATGTCTTCAACCTCGGCGAGAATCTCACTCCGAGATATAAAATCCTCAGCAAGATGGGTGAAGGCACATTTGGTCGAGTTTTGGAATGCTGGGACCGGCAAACTCGAGAATATGTAGCCATTAAGGTAGTTCGGAGCATAAGAAAGTACCGTGATGCAGCCATGGTTGAGGTGGATATACTTAAGCATCTTGCTCAGAATGACACAGGCTGCTTACGTTGTGTGCAGATTAGAAATTGGTTTGACTACCGCAATCACATATGTATAGTATTTGAGAGGCTTGGACCAAGCTTATTTGATTTTCTAAAGAGAAATAAATACCACCCATTCCCTGTGGATCTTGTTCGGGAGTTTGGACGACAGCTTTTGGAATCTGTAGCATATATGCATGATTTACACTTAATCCACACAGACCTGAAGCCAGAAAATATACTTCTTGTGTCTTCTGAATATATAAAGCTTCCTGGTTGTAAGAGGGTTTCGTCGGATGAAACGCAATTCAGGTGCTTGCCCAAGTCTAGTGCAATTAAGCTGATTGATTTTGGTAGTACTGCATTTGATAATGAGAATCATAGCTCCATTGTTTCTACGAGGCATTACAGAGCCCCTGAGGTTATTCTAGGTCTTGGATGGAGTTATCCATGCGATTTGTGGAGTATCGGTTGCATTCTTGTCGAACTGTGTTCGGGCAAAGCACTGTTTCAAACTCACGAGAACTTGGAGCATTTGGCAATGATGGAGAGGGTGTTGGGACCTATACCAGGGCACATGATTCAGAGTGCTGACCAAAACGCAGAAAAATATTTCAAACGAGGTTTGCGTCTAAACTGGCCCGAAGGAGCAGTTTCAAGAGAAAGCATTCGAGCCGTGAAGAAACTCGATCGTTTGAAGGATATGGTTTCACAACATGTGGGTTTCTCCAGGCTATTGACTGATCTGTTGTATGATCTATTGAAATACGACCCTTCGGAACGCCCAACAGCTCGACAAGCCCTTAACCATTCTTTCTTTAAGAGTATAGCATGAGAAGGTACGATTCCATGCTTAGTTACAGTTGCAATACAAGTCTGTCTGCAAAATGCTGGCTTTTTTATTTCAGGGGAAAGAGAGAGAGAGATGATTAAGAATGCTATGCAGACAATGATTTTTACTTGTATTTGTAGAAGTATGATTATTAATGAAAAGCATGTCA

Coding sequence (CDS)

ATGGAAGCTGAGAGAATGGAGAAAGAACAACAGCGGACCACCAGGAAAAGGCCTCGTTCCGCATGGGATGTGGTGCCATCCGAACAAGAGGCTAATAAGGCTTTGGCGGTGGTTAAGGATGAGGTCAAAAGGCATGCCTCGCCCCCGAGAAGAGACGATGATCGTGAAGGACACTATGTCTTCAACCTCGGCGAGAATCTCACTCCGAGATATAAAATCCTCAGCAAGATGGGTGAAGGCACATTTGGTCGAGTTTTGGAATGCTGGGACCGGCAAACTCGAGAATATGTAGCCATTAAGGTAGTTCGGAGCATAAGAAAGTACCGTGATGCAGCCATGGTTGAGGTGGATATACTTAAGCATCTTGCTCAGAATGACACAGGCTGCTTACGTTGTGTGCAGATTAGAAATTGGTTTGACTACCGCAATCACATATGTATAGTATTTGAGAGGCTTGGACCAAGCTTATTTGATTTTCTAAAGAGAAATAAATACCACCCATTCCCTGTGGATCTTGTTCGGGAGTTTGGACGACAGCTTTTGGAATCTGTAGCATATATGCATGATTTACACTTAATCCACACAGACCTGAAGCCAGAAAATATACTTCTTGTGTCTTCTGAATATATAAAGCTTCCTGGTTGTAAGAGGGTTTCGTCGGATGAAACGCAATTCAGGTGCTTGCCCAAGTCTAGTGCAATTAAGCTGATTGATTTTGGTAGTACTGCATTTGATAATGAGAATCATAGCTCCATTGTTTCTACGAGGCATTACAGAGCCCCTGAGGTTATTCTAGGTCTTGGATGGAGTTATCCATGCGATTTGTGGAGTATCGGTTGCATTCTTGTCGAACTGTGTTCGGGCAAAGCACTGTTTCAAACTCACGAGAACTTGGAGCATTTGGCAATGATGGAGAGGGTGTTGGGACCTATACCAGGGCACATGATTCAGAGTGCTGACCAAAACGCAGAAAAATATTTCAAACGAGGTTTGCGTCTAAACTGGCCCGAAGGAGCAGTTTCAAGAGAAAGCATTCGAGCCGTGAAGAAACTCGATCGTTTGAAGGATATGGTTTCACAACATGTGGGTTTCTCCAGGCTATTGACTGATCTGTTGTATGATCTATTGAAATACGACCCTTCGGAACGCCCAACAGCTCGACAAGCCCTTAACCATTCTTTCTTTAAGAGTATAGCATGA

Protein sequence

MEAERMEKEQQRTTRKRPRSAWDVVPSEQEANKALAVVKDEVKRHASPPRRDDDREGHYVFNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDILKHLAQNDTGCLRCVQIRNWFDYRNHICIVFERLGPSLFDFLKRNKYHPFPVDLVREFGRQLLESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKRVSSDETQFRCLPKSSAIKLIDFGSTAFDNENHSSIVSTRHYRAPEVILGLGWSYPCDLWSIGCILVELCSGKALFQTHENLEHLAMMERVLGPIPGHMIQSADQNAEKYFKRGLRLNWPEGAVSRESIRAVKKLDRLKDMVSQHVGFSRLLTDLLYDLLKYDPSERPTARQALNHSFFKSIA

Homology

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BLAST of Carg08294 vs. NCBI nr
Match: XP_023545797.1 (serine/threonine-protein kinase AFC3 isoform X1 [Cucurbita pepo subsp. pepo] >KAG6598793.1 Serine/threonine-protein kinase AFC3, partial [Cucurbita argyrosperma subsp. sororia] >KAG7029731.1 Serine/threonine-protein kinase AFC3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 820.8 bits (2119), Expect = 4.9e-234
Identity = 399/399 (100.00%), Postives = 399/399 (100.00%), Query Frame = 0


Query: 1   MEAERMEKEQQRTTRKRPRSAWDVVPSEQEANKALAVVKDEVKRHASPPRRDDDREGHYV 60
           MEAERMEKEQQRTTRKRPRSAWDVVPSEQEANKALAVVKDEVKRHASPPRRDDDREGHYV
Sbjct: 1   MEAERMEKEQQRTTRKRPRSAWDVVPSEQEANKALAVVKDEVKRHASPPRRDDDREGHYV 60

Query: 61  FNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDILK 120
           FNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDILK
Sbjct: 61  FNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDILK 120

Query: 121 HLAQNDTGCLRCVQIRNWFDYRNHICIVFERLGPSLFDFLKRNKYHPFPVDLVREFGRQL 180
           HLAQNDTGCLRCVQIRNWFDYRNHICIVFERLGPSLFDFLKRNKYHPFPVDLVREFGRQL
Sbjct: 121 HLAQNDTGCLRCVQIRNWFDYRNHICIVFERLGPSLFDFLKRNKYHPFPVDLVREFGRQL 180

Query: 181 LESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKRVSSDETQFRCLPKSSAIKLIDFG 240
           LESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKRVSSDETQFRCLPKSSAIKLIDFG
Sbjct: 181 LESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKRVSSDETQFRCLPKSSAIKLIDFG 240

Query: 241 STAFDNENHSSIVSTRHYRAPEVILGLGWSYPCDLWSIGCILVELCSGKALFQTHENLEH 300
           STAFDNENHSSIVSTRHYRAPEVILGLGWSYPCDLWSIGCILVELCSGKALFQTHENLEH
Sbjct: 241 STAFDNENHSSIVSTRHYRAPEVILGLGWSYPCDLWSIGCILVELCSGKALFQTHENLEH 300

Query: 301 LAMMERVLGPIPGHMIQSADQNAEKYFKRGLRLNWPEGAVSRESIRAVKKLDRLKDMVSQ 360
           LAMMERVLGPIPGHMIQSADQNAEKYFKRGLRLNWPEGAVSRESIRAVKKLDRLKDMVSQ
Sbjct: 301 LAMMERVLGPIPGHMIQSADQNAEKYFKRGLRLNWPEGAVSRESIRAVKKLDRLKDMVSQ 360

Query: 361 HVGFSRLLTDLLYDLLKYDPSERPTARQALNHSFFKSIA 400
           HVGFSRLLTDLLYDLLKYDPSERPTARQALNHSFFKSIA
Sbjct: 361 HVGFSRLLTDLLYDLLKYDPSERPTARQALNHSFFKSIA 399

[Close]

BLAST of Carg08294 vs. NCBI nr
Match: XP_022962075.1 (serine/threonine-protein kinase AFC3 isoform X1 [Cucurbita moschata])

HSP 1 Score: 818.9 bits (2114), Expect = 1.9e-233
Identity = 398/399 (99.75%), Postives = 398/399 (99.75%), Query Frame = 0


Query: 1   MEAERMEKEQQRTTRKRPRSAWDVVPSEQEANKALAVVKDEVKRHASPPRRDDDREGHYV 60
           MEAERMEKEQQRTTRKRPRSAWDVVPSEQEANKALAVVKDEVKRHASPPRRDDDREGHYV
Sbjct: 1   MEAERMEKEQQRTTRKRPRSAWDVVPSEQEANKALAVVKDEVKRHASPPRRDDDREGHYV 60

Query: 61  FNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDILK 120
           FNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDILK
Sbjct: 61  FNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDILK 120

Query: 121 HLAQNDTGCLRCVQIRNWFDYRNHICIVFERLGPSLFDFLKRNKYHPFPVDLVREFGRQL 180
           HLAQNDTGCLRCVQIRNWFDYRNHICIVFERLGPSLFDFLKRNKYHPFPVDLVREFGRQL
Sbjct: 121 HLAQNDTGCLRCVQIRNWFDYRNHICIVFERLGPSLFDFLKRNKYHPFPVDLVREFGRQL 180

Query: 181 LESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKRVSSDETQFRCLPKSSAIKLIDFG 240
           LESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKRVSSDETQFRCLPKSSAIKLIDFG
Sbjct: 181 LESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKRVSSDETQFRCLPKSSAIKLIDFG 240

Query: 241 STAFDNENHSSIVSTRHYRAPEVILGLGWSYPCDLWSIGCILVELCSGKALFQTHENLEH 300
           STAFDNENHSSIVSTRHYRAPEVILGLGWSYPCDLWSIGCILVELCSGKALFQTHENLEH
Sbjct: 241 STAFDNENHSSIVSTRHYRAPEVILGLGWSYPCDLWSIGCILVELCSGKALFQTHENLEH 300

Query: 301 LAMMERVLGPIPGHMIQSADQNAEKYFKRGLRLNWPEGAVSRESIRAVKKLDRLKDMVSQ 360
           LAMMERVLGPIPGHMIQSADQNAEKYFKRGL LNWPEGAVSRESIRAVKKLDRLKDMVSQ
Sbjct: 301 LAMMERVLGPIPGHMIQSADQNAEKYFKRGLHLNWPEGAVSRESIRAVKKLDRLKDMVSQ 360

Query: 361 HVGFSRLLTDLLYDLLKYDPSERPTARQALNHSFFKSIA 400
           HVGFSRLLTDLLYDLLKYDPSERPTARQALNHSFFKSIA
Sbjct: 361 HVGFSRLLTDLLYDLLKYDPSERPTARQALNHSFFKSIA 399

[Close]

BLAST of Carg08294 vs. NCBI nr
Match: XP_022996479.1 (serine/threonine-protein kinase AFC3 isoform X1 [Cucurbita maxima])

HSP 1 Score: 817.4 bits (2110), Expect = 5.5e-233
Identity = 397/399 (99.50%), Postives = 398/399 (99.75%), Query Frame = 0


Query: 1   MEAERMEKEQQRTTRKRPRSAWDVVPSEQEANKALAVVKDEVKRHASPPRRDDDREGHYV 60
           MEAERMEKEQQRTTRKRPRSAWDVVPSEQEANKAL VVKDEVKRHASPPRRDDDREGHYV
Sbjct: 4   MEAERMEKEQQRTTRKRPRSAWDVVPSEQEANKALVVVKDEVKRHASPPRRDDDREGHYV 63

Query: 61  FNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDILK 120
           FNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDILK
Sbjct: 64  FNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDILK 123

Query: 121 HLAQNDTGCLRCVQIRNWFDYRNHICIVFERLGPSLFDFLKRNKYHPFPVDLVREFGRQL 180
           HLAQNDTGCLRCVQIRNWFDYRNHICIVFERLGPSLFDFLKRNKYHPFPVDLVREFGRQL
Sbjct: 124 HLAQNDTGCLRCVQIRNWFDYRNHICIVFERLGPSLFDFLKRNKYHPFPVDLVREFGRQL 183

Query: 181 LESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKRVSSDETQFRCLPKSSAIKLIDFG 240
           LESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKRVSSDETQFRCLPKSSAIKLIDFG
Sbjct: 184 LESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKRVSSDETQFRCLPKSSAIKLIDFG 243

Query: 241 STAFDNENHSSIVSTRHYRAPEVILGLGWSYPCDLWSIGCILVELCSGKALFQTHENLEH 300
           STAFDNENHSSIVSTRHYRAPEVILGLGWSYPCDLWSIGCILVELCSGKALFQTHENLEH
Sbjct: 244 STAFDNENHSSIVSTRHYRAPEVILGLGWSYPCDLWSIGCILVELCSGKALFQTHENLEH 303

Query: 301 LAMMERVLGPIPGHMIQSADQNAEKYFKRGLRLNWPEGAVSRESIRAVKKLDRLKDMVSQ 360
           LAMMERVLGPIPGHMIQSADQNAEKYFKRGLRLNWPEGAVSRESIRAVKKLDRLKDMVSQ
Sbjct: 304 LAMMERVLGPIPGHMIQSADQNAEKYFKRGLRLNWPEGAVSRESIRAVKKLDRLKDMVSQ 363

Query: 361 HVGFSRLLTDLLYDLLKYDPSERPTARQALNHSFFKSIA 400
           HVGFSRLLTDLLYDLLKYDPSERPTARQAL+HSFFKSIA
Sbjct: 364 HVGFSRLLTDLLYDLLKYDPSERPTARQALDHSFFKSIA 402

[Close]

BLAST of Carg08294 vs. NCBI nr
Match: XP_038886114.1 (serine/threonine-protein kinase AFC3 [Benincasa hispida])

HSP 1 Score: 791.2 bits (2042), Expect = 4.2e-225
Identity = 387/400 (96.75%), Postives = 393/400 (98.25%), Query Frame = 0


Query: 1   MEAERMEKEQQRTTRKRPRSAWDVVPSEQEANKALAVVKDEVKRHASPPRRDDDREGHYV 60
           MEAERM KEQQRTTRKRPRSAWDVVPSEQEANKAL VVK++VKRHASPPRRDDDREGHYV
Sbjct: 1   MEAERMGKEQQRTTRKRPRSAWDVVPSEQEANKALVVVKNDVKRHASPPRRDDDREGHYV 60

Query: 61  FNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDILK 120
           FNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDILK
Sbjct: 61  FNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDILK 120

Query: 121 HLAQNDTGCLRCVQIRNWFDYRNHICIVFERLGPSLFDFLKRNKYHPFPVDLVREFGRQL 180
           HLAQNDTG LRCVQIR WFDYRNHICIVFE+LGPSLFDFLKRN+Y PFPVDLVREFGRQL
Sbjct: 121 HLAQNDTGSLRCVQIRTWFDYRNHICIVFEKLGPSLFDFLKRNRYCPFPVDLVREFGRQL 180

Query: 181 LESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKRVSSDETQFRCLPKSSAIKLIDFG 240
           LESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKRVSSDETQFRCLPKSSAIKLIDFG
Sbjct: 181 LESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKRVSSDETQFRCLPKSSAIKLIDFG 240

Query: 241 STAFDNENHSSIVSTRHYRAPEVILGLGWSYPCDLWSIGCILVELCSGKALFQTHENLEH 300
           STAFDNENHSSIVSTRHYRAPEVILGLGWSYPCDLWSIGCILVELCSGKALFQTHENLEH
Sbjct: 241 STAFDNENHSSIVSTRHYRAPEVILGLGWSYPCDLWSIGCILVELCSGKALFQTHENLEH 300

Query: 301 LAMMERVLGPIPGHMIQSADQNAEKYFKRGLRLNWPEGAVSRESIRAVKKLDRLKDMVSQ 360
           LAMMERVLGP+PGHMIQSADQNAEKYFKRGLRLNWPEGAVSRESIRAVKKLDRLKDMVSQ
Sbjct: 301 LAMMERVLGPLPGHMIQSADQNAEKYFKRGLRLNWPEGAVSRESIRAVKKLDRLKDMVSQ 360

Query: 361 HVGFSR-LLTDLLYDLLKYDPSERPTARQALNHSFFKSIA 400
           +VGFSR LLTDLLYDLLKYDPSERPTARQALNH FFKSIA
Sbjct: 361 YVGFSRSLLTDLLYDLLKYDPSERPTARQALNHPFFKSIA 400

[Close]

BLAST of Carg08294 vs. NCBI nr
Match: XP_022132125.1 (serine/threonine-protein kinase AFC3 [Momordica charantia])

HSP 1 Score: 789.6 bits (2038), Expect = 1.2e-224
Identity = 385/400 (96.25%), Postives = 392/400 (98.00%), Query Frame = 0


Query: 1   MEAERMEKEQQRTTRKRPRSAWDVVPSEQEANKALAVVKDEVKRHASPPRRDDDREGHYV 60
           MEA+RMEKEQQRTTRKRPRSAWDVVPSEQEA+KAL V KDEVKRHASPPRRDDDREGHYV
Sbjct: 4   MEADRMEKEQQRTTRKRPRSAWDVVPSEQEAHKALVVGKDEVKRHASPPRRDDDREGHYV 63

Query: 61  FNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDILK 120
           FNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDILK
Sbjct: 64  FNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDILK 123

Query: 121 HLAQNDTGCLRCVQIRNWFDYRNHICIVFERLGPSLFDFLKRNKYHPFPVDLVREFGRQL 180
           HLAQND G LRCVQIRNWFDYRNHICIVFE+LGPSLFDFLKRNKY PFPVDLVREFGRQL
Sbjct: 124 HLAQNDKGSLRCVQIRNWFDYRNHICIVFEKLGPSLFDFLKRNKYCPFPVDLVREFGRQL 183

Query: 181 LESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKRVSSDETQFRCLPKSSAIKLIDFG 240
           LESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCK+VSSDETQFRCLPKSSAIKLIDFG
Sbjct: 184 LESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKKVSSDETQFRCLPKSSAIKLIDFG 243

Query: 241 STAFDNENHSSIVSTRHYRAPEVILGLGWSYPCDLWSIGCILVELCSGKALFQTHENLEH 300
           STAFDNENHSSIVSTRHYRAPE+ILGLGWSYPCDLWSIGCILVELCSGKALFQTHENLEH
Sbjct: 244 STAFDNENHSSIVSTRHYRAPEIILGLGWSYPCDLWSIGCILVELCSGKALFQTHENLEH 303

Query: 301 LAMMERVLGPIPGHMIQSADQNAEKYFKRGLRLNWPEGAVSRESIRAVKKLDRLKDMVSQ 360
           LAMMERVLGP+PGHMIQSADQNAEKYFKRG RLNWPEGAVSRESIRAVKKLDRLKDMVSQ
Sbjct: 304 LAMMERVLGPLPGHMIQSADQNAEKYFKRGSRLNWPEGAVSRESIRAVKKLDRLKDMVSQ 363

Query: 361 HVGFSR-LLTDLLYDLLKYDPSERPTARQALNHSFFKSIA 400
           H+GFSR LLTDLLYDLLKYDPSERPTARQALNH FFKSIA
Sbjct: 364 HLGFSRSLLTDLLYDLLKYDPSERPTARQALNHPFFKSIA 403

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BLAST of Carg08294 vs. ExPASy Swiss-Prot
Match: P51568 (Serine/threonine-protein kinase AFC3 OS=Arabidopsis thaliana OX=3702 GN=AFC3 PE=1 SV=2)

HSP 1 Score: 594.0 bits (1530), Expect = 1.3e-168
Identity = 287/395 (72.66%), Postives = 329/395 (83.29%), Query Frame = 0


Query: 4   ERMEKEQQRTTRKRPRSAWDVVPSEQEANKALAVVKDEVKRHASPPRRDDDREGHYVFNL 63
           E M+KE+    RKRPR  WD  P+E EA +A+        R  SPP RDDDR+GHYVF+L
Sbjct: 7   ESMDKER---VRKRPRMTWDEAPAEPEAKRAVIKGHGSDGRILSPPLRDDDRDGHYVFSL 66

Query: 64  GENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDILKHLA 123
            +NLTPRYKILSKMGEGTFGRVLECWDR T+EYVAIK++RSI+KYRDAAM+E+D+L+ L 
Sbjct: 67  RDNLTPRYKILSKMGEGTFGRVLECWDRDTKEYVAIKIIRSIKKYRDAAMIEIDVLQKLV 126

Query: 124 QNDTGCLRCVQIRNWFDYRNHICIVFERLGPSLFDFLKRNKYHPFPVDLVREFGRQLLES 183
           ++D G  RCVQ++NWFDYRNHICIVFE+LGPSLFDFLKRNKY  FP+ LVR+FG QLLES
Sbjct: 127 KSDKGRTRCVQMKNWFDYRNHICIVFEKLGPSLFDFLKRNKYSAFPLALVRDFGCQLLES 186

Query: 184 VAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKRVSSDETQFRCLPKSSAIKLIDFGSTA 243
           VAYMH+L L+HTDLKPENILLVSSE +KLP  KR +++ET FRCLPKSSAIKLIDFGST 
Sbjct: 187 VAYMHELQLVHTDLKPENILLVSSENVKLPDNKRSAANETHFRCLPKSSAIKLIDFGSTV 246

Query: 244 FDNENHSSIVSTRHYRAPEVILGLGWSYPCDLWSIGCILVELCSGKALFQTHENLEHLAM 303
            DN  H SIV TRHYR+PEVILGLGWSY CDLWSIGCIL ELC+G+ALFQTH+NLEHLAM
Sbjct: 247 CDNRIHHSIVQTRHYRSPEVILGLGWSYQCDLWSIGCILFELCTGEALFQTHDNLEHLAM 306

Query: 304 MERVLGPIPGHMIQSADQNAEKYFKRGLRLNWPEGAVSRESIRAVKKLDRLKDMVSQHVG 363
           MER LGP+P HM + A + AEKYF+RG RLNWPEGA SRESIRAVK+LDRLKDMVS+HV 
Sbjct: 307 MERALGPLPEHMTRKASRGAEKYFRRGCRLNWPEGANSRESIRAVKRLDRLKDMVSKHVD 366

Query: 364 FSR-LLTDLLYDLLKYDPSERPTARQALNHSFFKS 398
            +R    DLLY LL YDPSER TA +AL+H FFKS
Sbjct: 367 NTRSRFADLLYGLLAYDPSERLTANEALDHPFFKS 398

[Close]

BLAST of Carg08294 vs. ExPASy Swiss-Prot
Match: P51566 (Serine/threonine-protein kinase AFC1 OS=Arabidopsis thaliana OX=3702 GN=AFC1 PE=2 SV=2)

HSP 1 Score: 537.0 bits (1382), Expect = 1.9e-151
Identity = 262/409 (64.06%), Postives = 315/409 (77.02%), Query Frame = 0


Query: 15  RKRPRSAWDVVPSEQEANKALAVVK------------------------DEVKRHASPPR 74
           RKRPR  WD  P          V +                        + + R  SPP 
Sbjct: 35  RKRPRLTWDAAPPLLPPPPPPTVFQPPLYYGPEFASGLVPNFVYPNMFYNGLPRQGSPPW 94

Query: 75  RDDDREGHYVFNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRD 134
           R DD++GHYVF +G+ LTPRY+ILSKMGEGTFG+VLEC+D + +E VAIKV+RSI KYR+
Sbjct: 95  RPDDKDGHYVFVVGDTLTPRYQILSKMGEGTFGQVLECFDNKNKEVVAIKVIRSINKYRE 154

Query: 135 AAMVEVDILKHLAQNDTGCLRCVQIRNWFDYRNHICIVFERLGPSLFDFLKRNKYHPFPV 194
           AAM+E+D+L+ L ++D G  RCVQIRNWFDYRNHICIVFE+LGPSL+DFL++N Y  FP+
Sbjct: 155 AAMIEIDVLQRLTRHDVGGSRCVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNSYRSFPI 214

Query: 195 DLVREFGRQLLESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCK---RVSSDETQFRC 254
           DLVRE GRQLLESVAYMHDL LIHTDLKPENILLVSSEYIK+P  K   R + D + F+ 
Sbjct: 215 DLVRELGRQLLESVAYMHDLRLIHTDLKPENILLVSSEYIKIPDYKFLSRPTKDGSYFKN 274

Query: 255 LPKSSAIKLIDFGSTAFDNENHSSIVSTRHYRAPEVILGLGWSYPCDLWSIGCILVELCS 314
           LPKSSAIKLIDFGST F++++H+ IVSTRHYRAPEVILG+GW+YPCDLWSIGCILVELCS
Sbjct: 275 LPKSSAIKLIDFGSTTFEHQDHNYIVSTRHYRAPEVILGVGWNYPCDLWSIGCILVELCS 334

Query: 315 GKALFQTHENLEHLAMMERVLGPIPGHMIQSADQNAEKYFKRGLRLNWPEGAVSRESIRA 374
           G+ALFQTHENLEHLAMMERVLGP+P HM+  AD+ +EKYF+RG +L+WPEGA SR+S++A
Sbjct: 335 GEALFQTHENLEHLAMMERVLGPLPPHMVLRADRRSEKYFRRGAKLDWPEGATSRDSLKA 394

Query: 375 VKKLDRLKDMVSQHVGFSR-LLTDLLYDLLKYDPSERPTARQALNHSFF 396
           V KL RL +++ QHV  S   L DLL  LL+YDP+ER  AR+ALNH FF
Sbjct: 395 VWKLPRLPNLIMQHVDHSAGDLIDLLQGLLRYDPTERFKAREALNHPFF 443

[Close]

BLAST of Carg08294 vs. ExPASy Swiss-Prot
Match: P51567 (Serine/threonine-protein kinase AFC2 OS=Arabidopsis thaliana OX=3702 GN=AFC2 PE=1 SV=1)

HSP 1 Score: 508.1 bits (1307), Expect = 9.3e-143
Identity = 249/426 (58.45%), Postives = 322/426 (75.59%), Query Frame = 0


Query: 1   MEAERMEK----EQQRTTRKRPRSAWDVVPSEQEA------------------------N 60
           ME ER+ +       R  RKR R  WDV+P   +A                        N
Sbjct: 1   MEMERVHEFPHTHMDRRPRKRARLGWDVLPQATKAQVGMFCGQEIGNISSFASSGAPSDN 60

Query: 61  KALAVVKDEVKRHASPPRRDDDREGHYVFNLGENLTPRYKILSKMGEGTFGRVLECWDRQ 120
            +   VK  V R+ SPP R+DD++GHY+F LG++LTPRYKI SKMGEGTFG+VLECWDR+
Sbjct: 61  SSSLCVKG-VARNGSPPWREDDKDGHYIFELGDDLTPRYKIYSKMGEGTFGQVLECWDRE 120

Query: 121 TREYVAIKVVRSIRKYRDAAMVEVDILKHLAQNDTGCLRCVQIRNWFDYRNHICIVFERL 180
            +E VA+K+VR ++KYR+AAM+E+++L+ L ++D G  RCVQIRNWFDYRNHICIVFE+L
Sbjct: 121 RKEMVAVKIVRGVKKYREAAMIEIEMLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180

Query: 181 GPSLFDFLKRNKYHPFPVDLVREFGRQLLESVAYMHDLHLIHTDLKPENILLVSSEYIKL 240
           G SL+DFL++N Y  FP+DLVRE G QLLE VA+MHDL +IHTDLKPENILLVSS+Y+K+
Sbjct: 181 GSSLYDFLRKNNYRSFPIDLVREIGWQLLECVAFMHDLRMIHTDLKPENILLVSSDYVKI 240

Query: 241 PGCK--RVSSDETQFRCLPKSSAIKLIDFGSTAFDNENHSSIVSTRHYRAPEVILGLGWS 300
           P  K  R+  D   ++ +PKSSAIK+IDFGST ++ ++ + IVSTRHYRAPEVILGLGWS
Sbjct: 241 PEYKGSRLQRD-VCYKRVPKSSAIKVIDFGSTTYERQDQTYIVSTRHYRAPEVILGLGWS 300

Query: 301 YPCDLWSIGCILVELCSGKALFQTHENLEHLAMMERVLGPIPGHMIQSADQNAEKYFKRG 360
           YPCD+WS+GCI+VELC+G+ALFQTHENLEHLAMMERVLGP P  M++  D+++EKY +RG
Sbjct: 301 YPCDVWSVGCIIVELCTGEALFQTHENLEHLAMMERVLGPFPQQMLKKVDRHSEKYVRRG 360

Query: 361 LRLNWPEGAVSRESIRAVKKLDRLKDMVSQHVGFSR-LLTDLLYDLLKYDPSERPTARQA 396
            RL+WP+GA SR+S++AV KL RL++++ QHV  S   L +++  LL++DPSER TAR+A
Sbjct: 361 -RLDWPDGATSRDSLKAVLKLPRLQNLIMQHVDHSAGELINMVQGLLRFDPSERITAREA 420

[Close]

BLAST of Carg08294 vs. ExPASy Swiss-Prot
Match: Q10156 (Dual specificity protein kinase lkh1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=lkh1 PE=1 SV=3)

HSP 1 Score: 347.8 bits (891), Expect = 1.6e-94
Identity = 179/387 (46.25%), Postives = 251/387 (64.86%), Query Frame = 0


Query: 12  RTTRKRPRSAWDVVPSEQEANKALAVVKDEVKRHASPPRRDDDREGHYVFNLGENLTPRY 71
           R  +KR R+AW         N  + VV D     A  P   DD +GHY          RY
Sbjct: 308 RPPKKRKRAAWPPYKKVDRVN--VPVVHDTT---AFDPSTFDDDDGHYKVVPNSKFANRY 367

Query: 72  KILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDILKHLAQND-TGCL 131
            ++  +G GTFG+V++C+D+ T  + AIKV R+I KYR+A+++E+ +L+ +A +D T   
Sbjct: 368 TVVRLLGHGTFGKVIQCYDQSTGRHCAIKVTRAIPKYREASLIELRVLQTIAHSDPTNEN 427

Query: 132 RCVQIRNWFDYRNHICIVFERLGPSLFDFLKRNKYHPFPVDLVREFGRQLLESVAYMHDL 191
           +C+Q+R++FDYR HICIV +  G S+FDFLK N Y PFP+  ++   +QL +SVA++H L
Sbjct: 428 KCIQLRDYFDYRKHICIVTDLFGWSVFDFLKNNNYIPFPLKHIQMLSQQLFKSVAFLHSL 487

Query: 192 HLIHTDLKPENILLVS--SEYIKLPGCKRVSSDETQFRCLPKSSAIKLIDFGSTAFDNEN 251
            L+HTDLKPEN+LLVS  S  I+LP   R  S +    C      I+LIDFGS  F++E 
Sbjct: 488 GLVHTDLKPENVLLVSNASRTIRLP--YRNYSQKVLNSC-----EIRLIDFGSATFEDEY 547

Query: 252 HSSIVSTRHYRAPEVILGLGWSYPCDLWSIGCILVELCSGKALFQTHENLEHLAMMERVL 311
           HSS+VSTRHYRAPE+ILGLGWSYPCD+WSIGCILVEL +G+ALFQTHE+ EHL MME++L
Sbjct: 548 HSSVVSTRHYRAPEIILGLGWSYPCDVWSIGCILVELFTGQALFQTHEDSEHLCMMEKIL 607

Query: 312 GPIPGHMIQSADQNAEKYFKRGLRLNWPEGAVSRESIRAVKKLDRLKDMVSQHVGFSRLL 371
           GP   +MI  + + ++++FK   ++ +P     ++SI  ++ L  L+ + +       LL
Sbjct: 608 GPFDRNMISRSSRTSQRFFKSDGKVRYPLSNTPKKSINYLQSLQTLEQIFAVSSPEVALL 667

Query: 372 TDLLYDLLKYDPSERPTARQALNHSFF 396
            DLL  +  YDP  R TA++AL H FF
Sbjct: 668 LDLLKKVFVYDPKRRITAKEALWHPFF 682

[Close]

BLAST of Carg08294 vs. ExPASy Swiss-Prot
Match: O35491 (Dual specificity protein kinase CLK2 OS=Mus musculus OX=10090 GN=Clk2 PE=1 SV=2)

HSP 1 Score: 332.4 bits (851), Expect = 7.0e-90
Identity = 162/346 (46.82%), Postives = 234/346 (67.63%), Query Frame = 0


Query: 52  DDDREGHYVFNLGENLTPRYKILSKMGEGTFGRVLECWD-RQTREYVAIKVVRSIRKYRD 111
           +DD EGH ++++G+ L  RY+I+S +GEGTFGRV++C D R+    VA+K+++++ KY++
Sbjct: 143 EDDAEGHLIYHVGDWLQERYEIVSTLGEGTFGRVVQCVDHRRGGTQVALKIIKNVEKYKE 202

Query: 112 AAMVEVDILKHLAQNDTGCLR-CVQIRNWFDYRNHICIVFERLGPSLFDFLKRNKYHPFP 171
           AA +E+++L+ + + D      CVQ+ +WFDY  H+CI FE LG S FDFLK N Y P+P
Sbjct: 203 AARLEINVLEKINEKDPDNKNLCVQMFDWFDYHGHMCISFELLGLSTFDFLKDNNYLPYP 262

Query: 172 VDLVREFGRQLLESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKRVSSDETQFRCLP 231
           +  VR    QL ++V ++HD  L HTDLKPENIL V+S+Y      ++   + +      
Sbjct: 263 IHQVRHMAFQLCQAVKFLHDNKLTHTDLKPENILFVNSDYELTYNLEKKRDERS-----V 322

Query: 232 KSSAIKLIDFGSTAFDNENHSSIVSTRHYRAPEVILGLGWSYPCDLWSIGCILVELCSGK 291
           KS+A++++DFGS  FD+E+HS+IVSTRHYRAPEVIL LGWS PCD+WSIGCI+ E   G 
Sbjct: 323 KSTAVRVVDFGSATFDHEHHSTIVSTRHYRAPEVILELGWSQPCDVWSIGCIIFEYYVGF 382

Query: 292 ALFQTHENLEHLAMMERVLGPIPGHMIQSADQNAEKYFKRGLRLNWPEGAVSRESIRAVK 351
            LFQTH+N EHLAMMER+LGP+P  MI+      +KYF RG RL+W E   +   +R  +
Sbjct: 383 TLFQTHDNREHLAMMERILGPVPSRMIRKT--RKQKYFYRG-RLDWDENTSAGRYVR--E 442

Query: 352 KLDRLKDMVSQHVGFSRLLTDLLYDLLKYDPSERPTARQALNHSFF 396
               L+  ++        L DL+ ++L+Y+P++R T  +AL H FF
Sbjct: 443 NCKPLRRYLTSEAEDHHQLFDLIENMLEYEPAKRLTLGEALQHPFF 478

[Close]

BLAST of Carg08294 vs. ExPASy TrEMBL
Match: A0A6J1HE30 (serine/threonine-protein kinase AFC3 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111462640 PE=4 SV=1)

HSP 1 Score: 818.9 bits (2114), Expect = 9.1e-234
Identity = 398/399 (99.75%), Postives = 398/399 (99.75%), Query Frame = 0


Query: 1   MEAERMEKEQQRTTRKRPRSAWDVVPSEQEANKALAVVKDEVKRHASPPRRDDDREGHYV 60
           MEAERMEKEQQRTTRKRPRSAWDVVPSEQEANKALAVVKDEVKRHASPPRRDDDREGHYV
Sbjct: 1   MEAERMEKEQQRTTRKRPRSAWDVVPSEQEANKALAVVKDEVKRHASPPRRDDDREGHYV 60

Query: 61  FNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDILK 120
           FNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDILK
Sbjct: 61  FNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDILK 120

Query: 121 HLAQNDTGCLRCVQIRNWFDYRNHICIVFERLGPSLFDFLKRNKYHPFPVDLVREFGRQL 180
           HLAQNDTGCLRCVQIRNWFDYRNHICIVFERLGPSLFDFLKRNKYHPFPVDLVREFGRQL
Sbjct: 121 HLAQNDTGCLRCVQIRNWFDYRNHICIVFERLGPSLFDFLKRNKYHPFPVDLVREFGRQL 180

Query: 181 LESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKRVSSDETQFRCLPKSSAIKLIDFG 240
           LESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKRVSSDETQFRCLPKSSAIKLIDFG
Sbjct: 181 LESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKRVSSDETQFRCLPKSSAIKLIDFG 240

Query: 241 STAFDNENHSSIVSTRHYRAPEVILGLGWSYPCDLWSIGCILVELCSGKALFQTHENLEH 300
           STAFDNENHSSIVSTRHYRAPEVILGLGWSYPCDLWSIGCILVELCSGKALFQTHENLEH
Sbjct: 241 STAFDNENHSSIVSTRHYRAPEVILGLGWSYPCDLWSIGCILVELCSGKALFQTHENLEH 300

Query: 301 LAMMERVLGPIPGHMIQSADQNAEKYFKRGLRLNWPEGAVSRESIRAVKKLDRLKDMVSQ 360
           LAMMERVLGPIPGHMIQSADQNAEKYFKRGL LNWPEGAVSRESIRAVKKLDRLKDMVSQ
Sbjct: 301 LAMMERVLGPIPGHMIQSADQNAEKYFKRGLHLNWPEGAVSRESIRAVKKLDRLKDMVSQ 360

Query: 361 HVGFSRLLTDLLYDLLKYDPSERPTARQALNHSFFKSIA 400
           HVGFSRLLTDLLYDLLKYDPSERPTARQALNHSFFKSIA
Sbjct: 361 HVGFSRLLTDLLYDLLKYDPSERPTARQALNHSFFKSIA 399

[Close]

BLAST of Carg08294 vs. ExPASy TrEMBL
Match: A0A6J1K8U5 (serine/threonine-protein kinase AFC3 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111491714 PE=4 SV=1)

HSP 1 Score: 817.4 bits (2110), Expect = 2.6e-233
Identity = 397/399 (99.50%), Postives = 398/399 (99.75%), Query Frame = 0


Query: 1   MEAERMEKEQQRTTRKRPRSAWDVVPSEQEANKALAVVKDEVKRHASPPRRDDDREGHYV 60
           MEAERMEKEQQRTTRKRPRSAWDVVPSEQEANKAL VVKDEVKRHASPPRRDDDREGHYV
Sbjct: 4   MEAERMEKEQQRTTRKRPRSAWDVVPSEQEANKALVVVKDEVKRHASPPRRDDDREGHYV 63

Query: 61  FNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDILK 120
           FNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDILK
Sbjct: 64  FNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDILK 123

Query: 121 HLAQNDTGCLRCVQIRNWFDYRNHICIVFERLGPSLFDFLKRNKYHPFPVDLVREFGRQL 180
           HLAQNDTGCLRCVQIRNWFDYRNHICIVFERLGPSLFDFLKRNKYHPFPVDLVREFGRQL
Sbjct: 124 HLAQNDTGCLRCVQIRNWFDYRNHICIVFERLGPSLFDFLKRNKYHPFPVDLVREFGRQL 183

Query: 181 LESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKRVSSDETQFRCLPKSSAIKLIDFG 240
           LESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKRVSSDETQFRCLPKSSAIKLIDFG
Sbjct: 184 LESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKRVSSDETQFRCLPKSSAIKLIDFG 243

Query: 241 STAFDNENHSSIVSTRHYRAPEVILGLGWSYPCDLWSIGCILVELCSGKALFQTHENLEH 300
           STAFDNENHSSIVSTRHYRAPEVILGLGWSYPCDLWSIGCILVELCSGKALFQTHENLEH
Sbjct: 244 STAFDNENHSSIVSTRHYRAPEVILGLGWSYPCDLWSIGCILVELCSGKALFQTHENLEH 303

Query: 301 LAMMERVLGPIPGHMIQSADQNAEKYFKRGLRLNWPEGAVSRESIRAVKKLDRLKDMVSQ 360
           LAMMERVLGPIPGHMIQSADQNAEKYFKRGLRLNWPEGAVSRESIRAVKKLDRLKDMVSQ
Sbjct: 304 LAMMERVLGPIPGHMIQSADQNAEKYFKRGLRLNWPEGAVSRESIRAVKKLDRLKDMVSQ 363

Query: 361 HVGFSRLLTDLLYDLLKYDPSERPTARQALNHSFFKSIA 400
           HVGFSRLLTDLLYDLLKYDPSERPTARQAL+HSFFKSIA
Sbjct: 364 HVGFSRLLTDLLYDLLKYDPSERPTARQALDHSFFKSIA 402

[Close]

BLAST of Carg08294 vs. ExPASy TrEMBL
Match: A0A6J1BRK0 (serine/threonine-protein kinase AFC3 OS=Momordica charantia OX=3673 GN=LOC111005069 PE=4 SV=1)

HSP 1 Score: 789.6 bits (2038), Expect = 5.9e-225
Identity = 385/400 (96.25%), Postives = 392/400 (98.00%), Query Frame = 0


Query: 1   MEAERMEKEQQRTTRKRPRSAWDVVPSEQEANKALAVVKDEVKRHASPPRRDDDREGHYV 60
           MEA+RMEKEQQRTTRKRPRSAWDVVPSEQEA+KAL V KDEVKRHASPPRRDDDREGHYV
Sbjct: 4   MEADRMEKEQQRTTRKRPRSAWDVVPSEQEAHKALVVGKDEVKRHASPPRRDDDREGHYV 63

Query: 61  FNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDILK 120
           FNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDILK
Sbjct: 64  FNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDILK 123

Query: 121 HLAQNDTGCLRCVQIRNWFDYRNHICIVFERLGPSLFDFLKRNKYHPFPVDLVREFGRQL 180
           HLAQND G LRCVQIRNWFDYRNHICIVFE+LGPSLFDFLKRNKY PFPVDLVREFGRQL
Sbjct: 124 HLAQNDKGSLRCVQIRNWFDYRNHICIVFEKLGPSLFDFLKRNKYCPFPVDLVREFGRQL 183

Query: 181 LESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKRVSSDETQFRCLPKSSAIKLIDFG 240
           LESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCK+VSSDETQFRCLPKSSAIKLIDFG
Sbjct: 184 LESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKKVSSDETQFRCLPKSSAIKLIDFG 243

Query: 241 STAFDNENHSSIVSTRHYRAPEVILGLGWSYPCDLWSIGCILVELCSGKALFQTHENLEH 300
           STAFDNENHSSIVSTRHYRAPE+ILGLGWSYPCDLWSIGCILVELCSGKALFQTHENLEH
Sbjct: 244 STAFDNENHSSIVSTRHYRAPEIILGLGWSYPCDLWSIGCILVELCSGKALFQTHENLEH 303

Query: 301 LAMMERVLGPIPGHMIQSADQNAEKYFKRGLRLNWPEGAVSRESIRAVKKLDRLKDMVSQ 360
           LAMMERVLGP+PGHMIQSADQNAEKYFKRG RLNWPEGAVSRESIRAVKKLDRLKDMVSQ
Sbjct: 304 LAMMERVLGPLPGHMIQSADQNAEKYFKRGSRLNWPEGAVSRESIRAVKKLDRLKDMVSQ 363

Query: 361 HVGFSR-LLTDLLYDLLKYDPSERPTARQALNHSFFKSIA 400
           H+GFSR LLTDLLYDLLKYDPSERPTARQALNH FFKSIA
Sbjct: 364 HLGFSRSLLTDLLYDLLKYDPSERPTARQALNHPFFKSIA 403

[Close]

BLAST of Carg08294 vs. ExPASy TrEMBL
Match: A0A0A0LM05 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G375780 PE=4 SV=1)

HSP 1 Score: 782.3 bits (2019), Expect = 9.4e-223
Identity = 383/400 (95.75%), Postives = 390/400 (97.50%), Query Frame = 0


Query: 1   MEAERMEKEQQRTTRKRPRSAWDVVPSEQEANKALAVVKDEVKRHASPPRRDDDREGHYV 60
           MEA+RMEKEQQRT+RKRPRSAWDVVP  QEANKALAVVK+EVKRH SPPRRDDDREGHYV
Sbjct: 1   MEADRMEKEQQRTSRKRPRSAWDVVPPAQEANKALAVVKNEVKRHVSPPRRDDDREGHYV 60

Query: 61  FNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDILK 120
           FNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDILK
Sbjct: 61  FNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDILK 120

Query: 121 HLAQNDTGCLRCVQIRNWFDYRNHICIVFERLGPSLFDFLKRNKYHPFPVDLVREFGRQL 180
           HLAQN+ G L CVQIR WFDYRNHICIVFE+LGPSLFDFLKRNKY PFPVDLVREFGRQL
Sbjct: 121 HLAQNEMGSLCCVQIRTWFDYRNHICIVFEKLGPSLFDFLKRNKYCPFPVDLVREFGRQL 180

Query: 181 LESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKRVSSDETQFRCLPKSSAIKLIDFG 240
           LESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKRVSSDETQFRCLPKSSAIKLIDFG
Sbjct: 181 LESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKRVSSDETQFRCLPKSSAIKLIDFG 240

Query: 241 STAFDNENHSSIVSTRHYRAPEVILGLGWSYPCDLWSIGCILVELCSGKALFQTHENLEH 300
           STAFDNENHSSIVSTRHYRAPEVILGLGWSYPCDLWSIGCILVELCSGKALFQTHENLEH
Sbjct: 241 STAFDNENHSSIVSTRHYRAPEVILGLGWSYPCDLWSIGCILVELCSGKALFQTHENLEH 300

Query: 301 LAMMERVLGPIPGHMIQSADQNAEKYFKRGLRLNWPEGAVSRESIRAVKKLDRLKDMVSQ 360
           LAMMERVLGP+PGHMIQSADQNAEKYFKRGLRLNWPEGAVSRESIRAVKKLDRLKDMVSQ
Sbjct: 301 LAMMERVLGPLPGHMIQSADQNAEKYFKRGLRLNWPEGAVSRESIRAVKKLDRLKDMVSQ 360

Query: 361 HVGFSR-LLTDLLYDLLKYDPSERPTARQALNHSFFKSIA 400
           +VGFSR LLTDLLYDLLKYDPSERPTARQALNH FFKSIA
Sbjct: 361 YVGFSRSLLTDLLYDLLKYDPSERPTARQALNHPFFKSIA 400

[Close]

BLAST of Carg08294 vs. ExPASy TrEMBL
Match: A0A1S3BCM1 (serine/threonine-protein kinase AFC3 OS=Cucumis melo OX=3656 GN=LOC103488204 PE=4 SV=1)

HSP 1 Score: 782.3 bits (2019), Expect = 9.4e-223
Identity = 383/400 (95.75%), Postives = 390/400 (97.50%), Query Frame = 0


Query: 1   MEAERMEKEQQRTTRKRPRSAWDVVPSEQEANKALAVVKDEVKRHASPPRRDDDREGHYV 60
           MEA+RMEKEQQR TRKRPRSAWDVVP  QEANKALAVVK+EVKRHASPPRRDDDREGHYV
Sbjct: 1   MEADRMEKEQQRITRKRPRSAWDVVPPAQEANKALAVVKNEVKRHASPPRRDDDREGHYV 60

Query: 61  FNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDILK 120
           FNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDILK
Sbjct: 61  FNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDILK 120

Query: 121 HLAQNDTGCLRCVQIRNWFDYRNHICIVFERLGPSLFDFLKRNKYHPFPVDLVREFGRQL 180
           HLAQN+ G LRCVQIR WFDYRNHICIVFE+LGPSLFDFLKRNKY PFPVDLVREFGRQL
Sbjct: 121 HLAQNEMGSLRCVQIRTWFDYRNHICIVFEKLGPSLFDFLKRNKYCPFPVDLVREFGRQL 180

Query: 181 LESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKRVSSDETQFRCLPKSSAIKLIDFG 240
           LESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKRVSSDETQFRCLPKSSAIKLIDFG
Sbjct: 181 LESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKRVSSDETQFRCLPKSSAIKLIDFG 240

Query: 241 STAFDNENHSSIVSTRHYRAPEVILGLGWSYPCDLWSIGCILVELCSGKALFQTHENLEH 300
           STAFDNENHSSIVSTRHYRAPEVILGLGWSYPCDLWSIGCILVELCSGKALFQTHENLEH
Sbjct: 241 STAFDNENHSSIVSTRHYRAPEVILGLGWSYPCDLWSIGCILVELCSGKALFQTHENLEH 300

Query: 301 LAMMERVLGPIPGHMIQSADQNAEKYFKRGLRLNWPEGAVSRESIRAVKKLDRLKDMVSQ 360
           LAMMERVLGP+PGHMIQSADQNAEKYFKRG RLNWPEGAVSRESIRAVKKLDRLKDMVSQ
Sbjct: 301 LAMMERVLGPLPGHMIQSADQNAEKYFKRGSRLNWPEGAVSRESIRAVKKLDRLKDMVSQ 360

Query: 361 HVGFSR-LLTDLLYDLLKYDPSERPTARQALNHSFFKSIA 400
           +VGFSR LLTDLLYDLLKYDPSERPTAR+ALNH FFKSIA
Sbjct: 361 YVGFSRSLLTDLLYDLLKYDPSERPTARRALNHPFFKSIA 400

[Close]

BLAST of Carg08294 vs. TAIR 10
Match: AT4G32660.1 (Protein kinase superfamily protein )

HSP 1 Score: 594.0 bits (1530), Expect = 9.1e-170
Identity = 287/395 (72.66%), Postives = 329/395 (83.29%), Query Frame = 0


Query: 4   ERMEKEQQRTTRKRPRSAWDVVPSEQEANKALAVVKDEVKRHASPPRRDDDREGHYVFNL 63
           E M+KE+    RKRPR  WD  P+E EA +A+        R  SPP RDDDR+GHYVF+L
Sbjct: 7   ESMDKER---VRKRPRMTWDEAPAEPEAKRAVIKGHGSDGRILSPPLRDDDRDGHYVFSL 66

Query: 64  GENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDILKHLA 123
            +NLTPRYKILSKMGEGTFGRVLECWDR T+EYVAIK++RSI+KYRDAAM+E+D+L+ L 
Sbjct: 67  RDNLTPRYKILSKMGEGTFGRVLECWDRDTKEYVAIKIIRSIKKYRDAAMIEIDVLQKLV 126

Query: 124 QNDTGCLRCVQIRNWFDYRNHICIVFERLGPSLFDFLKRNKYHPFPVDLVREFGRQLLES 183
           ++D G  RCVQ++NWFDYRNHICIVFE+LGPSLFDFLKRNKY  FP+ LVR+FG QLLES
Sbjct: 127 KSDKGRTRCVQMKNWFDYRNHICIVFEKLGPSLFDFLKRNKYSAFPLALVRDFGCQLLES 186

Query: 184 VAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKRVSSDETQFRCLPKSSAIKLIDFGSTA 243
           VAYMH+L L+HTDLKPENILLVSSE +KLP  KR +++ET FRCLPKSSAIKLIDFGST 
Sbjct: 187 VAYMHELQLVHTDLKPENILLVSSENVKLPDNKRSAANETHFRCLPKSSAIKLIDFGSTV 246

Query: 244 FDNENHSSIVSTRHYRAPEVILGLGWSYPCDLWSIGCILVELCSGKALFQTHENLEHLAM 303
            DN  H SIV TRHYR+PEVILGLGWSY CDLWSIGCIL ELC+G+ALFQTH+NLEHLAM
Sbjct: 247 CDNRIHHSIVQTRHYRSPEVILGLGWSYQCDLWSIGCILFELCTGEALFQTHDNLEHLAM 306

Query: 304 MERVLGPIPGHMIQSADQNAEKYFKRGLRLNWPEGAVSRESIRAVKKLDRLKDMVSQHVG 363
           MER LGP+P HM + A + AEKYF+RG RLNWPEGA SRESIRAVK+LDRLKDMVS+HV 
Sbjct: 307 MERALGPLPEHMTRKASRGAEKYFRRGCRLNWPEGANSRESIRAVKRLDRLKDMVSKHVD 366

Query: 364 FSR-LLTDLLYDLLKYDPSERPTARQALNHSFFKS 398
            +R    DLLY LL YDPSER TA +AL+H FFKS
Sbjct: 367 NTRSRFADLLYGLLAYDPSERLTANEALDHPFFKS 398

[Close]

BLAST of Carg08294 vs. TAIR 10
Match: AT4G32660.3 (Protein kinase superfamily protein )

HSP 1 Score: 593.6 bits (1529), Expect = 1.2e-169
Identity = 286/393 (72.77%), Postives = 328/393 (83.46%), Query Frame = 0


Query: 6   MEKEQQRTTRKRPRSAWDVVPSEQEANKALAVVKDEVKRHASPPRRDDDREGHYVFNLGE 65
           M+KE+    RKRPR  WD  P+E EA +A+        R  SPP RDDDR+GHYVF+L +
Sbjct: 1   MDKER---VRKRPRMTWDEAPAEPEAKRAVIKGHGSDGRILSPPLRDDDRDGHYVFSLRD 60

Query: 66  NLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDILKHLAQN 125
           NLTPRYKILSKMGEGTFGRVLECWDR T+EYVAIK++RSI+KYRDAAM+E+D+L+ L ++
Sbjct: 61  NLTPRYKILSKMGEGTFGRVLECWDRDTKEYVAIKIIRSIKKYRDAAMIEIDVLQKLVKS 120

Query: 126 DTGCLRCVQIRNWFDYRNHICIVFERLGPSLFDFLKRNKYHPFPVDLVREFGRQLLESVA 185
           D G  RCVQ++NWFDYRNHICIVFE+LGPSLFDFLKRNKY  FP+ LVR+FG QLLESVA
Sbjct: 121 DKGRTRCVQMKNWFDYRNHICIVFEKLGPSLFDFLKRNKYSAFPLALVRDFGCQLLESVA 180

Query: 186 YMHDLHLIHTDLKPENILLVSSEYIKLPGCKRVSSDETQFRCLPKSSAIKLIDFGSTAFD 245
           YMH+L L+HTDLKPENILLVSSE +KLP  KR +++ET FRCLPKSSAIKLIDFGST  D
Sbjct: 181 YMHELQLVHTDLKPENILLVSSENVKLPDNKRSAANETHFRCLPKSSAIKLIDFGSTVCD 240

Query: 246 NENHSSIVSTRHYRAPEVILGLGWSYPCDLWSIGCILVELCSGKALFQTHENLEHLAMME 305
           N  H SIV TRHYR+PEVILGLGWSY CDLWSIGCIL ELC+G+ALFQTH+NLEHLAMME
Sbjct: 241 NRIHHSIVQTRHYRSPEVILGLGWSYQCDLWSIGCILFELCTGEALFQTHDNLEHLAMME 300

Query: 306 RVLGPIPGHMIQSADQNAEKYFKRGLRLNWPEGAVSRESIRAVKKLDRLKDMVSQHVGFS 365
           R LGP+P HM + A + AEKYF+RG RLNWPEGA SRESIRAVK+LDRLKDMVS+HV  +
Sbjct: 301 RALGPLPEHMTRKASRGAEKYFRRGCRLNWPEGANSRESIRAVKRLDRLKDMVSKHVDNT 360

Query: 366 R-LLTDLLYDLLKYDPSERPTARQALNHSFFKS 398
           R    DLLY LL YDPSER TA +AL+H FFKS
Sbjct: 361 RSRFADLLYGLLAYDPSERLTANEALDHPFFKS 390

[Close]

BLAST of Carg08294 vs. TAIR 10
Match: AT4G32660.2 (Protein kinase superfamily protein )

HSP 1 Score: 544.3 bits (1401), Expect = 8.3e-155
Identity = 259/352 (73.58%), Postives = 297/352 (84.38%), Query Frame = 0


Query: 4   ERMEKEQQRTTRKRPRSAWDVVPSEQEANKALAVVKDEVKRHASPPRRDDDREGHYVFNL 63
           E M+KE+    RKRPR  WD  P+E EA +A+        R  SPP RDDDR+GHYVF+L
Sbjct: 7   ESMDKER---VRKRPRMTWDEAPAEPEAKRAVIKGHGSDGRILSPPLRDDDRDGHYVFSL 66

Query: 64  GENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRDAAMVEVDILKHLA 123
            +NLTPRYKILSKMGEGTFGRVLECWDR T+EYVAIK++RSI+KYRDAAM+E+D+L+ L 
Sbjct: 67  RDNLTPRYKILSKMGEGTFGRVLECWDRDTKEYVAIKIIRSIKKYRDAAMIEIDVLQKLV 126

Query: 124 QNDTGCLRCVQIRNWFDYRNHICIVFERLGPSLFDFLKRNKYHPFPVDLVREFGRQLLES 183
           ++D G  RCVQ++NWFDYRNHICIVFE+LGPSLFDFLKRNKY  FP+ LVR+FG QLLES
Sbjct: 127 KSDKGRTRCVQMKNWFDYRNHICIVFEKLGPSLFDFLKRNKYSAFPLALVRDFGCQLLES 186

Query: 184 VAYMHDLHLIHTDLKPENILLVSSEYIKLPGCKRVSSDETQFRCLPKSSAIKLIDFGSTA 243
           VAYMH+L L+HTDLKPENILLVSSE +KLP  KR +++ET FRCLPKSSAIKLIDFGST 
Sbjct: 187 VAYMHELQLVHTDLKPENILLVSSENVKLPDNKRSAANETHFRCLPKSSAIKLIDFGSTV 246

Query: 244 FDNENHSSIVSTRHYRAPEVILGLGWSYPCDLWSIGCILVELCSGKALFQTHENLEHLAM 303
            DN  H SIV TRHYR+PEVILGLGWSY CDLWSIGCIL ELC+G+ALFQTH+NLEHLAM
Sbjct: 247 CDNRIHHSIVQTRHYRSPEVILGLGWSYQCDLWSIGCILFELCTGEALFQTHDNLEHLAM 306

Query: 304 MERVLGPIPGHMIQSADQNAEKYFKRGLRLNWPEGAVSRESIRAVKKLDRLK 356
           MER LGP+P HM + A + AEKYF+RG RLNWPEGA SRESIRAVK+LDRLK
Sbjct: 307 MERALGPLPEHMTRKASRGAEKYFRRGCRLNWPEGANSRESIRAVKRLDRLK 355

[Close]

BLAST of Carg08294 vs. TAIR 10
Match: AT3G53570.3 (FUS3-complementing gene 1 )

HSP 1 Score: 537.0 bits (1382), Expect = 1.3e-152
Identity = 262/409 (64.06%), Postives = 315/409 (77.02%), Query Frame = 0


Query: 15  RKRPRSAWDVVPSEQEANKALAVVK------------------------DEVKRHASPPR 74
           RKRPR  WD  P          V +                        + + R  SPP 
Sbjct: 21  RKRPRLTWDAAPPLLPPPPPPTVFQPPLYYGPEFASGLVPNFVYPNMFYNGLPRQGSPPW 80

Query: 75  RDDDREGHYVFNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRD 134
           R DD++GHYVF +G+ LTPRY+ILSKMGEGTFG+VLEC+D + +E VAIKV+RSI KYR+
Sbjct: 81  RPDDKDGHYVFVVGDTLTPRYQILSKMGEGTFGQVLECFDNKNKEVVAIKVIRSINKYRE 140

Query: 135 AAMVEVDILKHLAQNDTGCLRCVQIRNWFDYRNHICIVFERLGPSLFDFLKRNKYHPFPV 194
           AAM+E+D+L+ L ++D G  RCVQIRNWFDYRNHICIVFE+LGPSL+DFL++N Y  FP+
Sbjct: 141 AAMIEIDVLQRLTRHDVGGSRCVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNSYRSFPI 200

Query: 195 DLVREFGRQLLESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCK---RVSSDETQFRC 254
           DLVRE GRQLLESVAYMHDL LIHTDLKPENILLVSSEYIK+P  K   R + D + F+ 
Sbjct: 201 DLVRELGRQLLESVAYMHDLRLIHTDLKPENILLVSSEYIKIPDYKFLSRPTKDGSYFKN 260

Query: 255 LPKSSAIKLIDFGSTAFDNENHSSIVSTRHYRAPEVILGLGWSYPCDLWSIGCILVELCS 314
           LPKSSAIKLIDFGST F++++H+ IVSTRHYRAPEVILG+GW+YPCDLWSIGCILVELCS
Sbjct: 261 LPKSSAIKLIDFGSTTFEHQDHNYIVSTRHYRAPEVILGVGWNYPCDLWSIGCILVELCS 320

Query: 315 GKALFQTHENLEHLAMMERVLGPIPGHMIQSADQNAEKYFKRGLRLNWPEGAVSRESIRA 374
           G+ALFQTHENLEHLAMMERVLGP+P HM+  AD+ +EKYF+RG +L+WPEGA SR+S++A
Sbjct: 321 GEALFQTHENLEHLAMMERVLGPLPPHMVLRADRRSEKYFRRGAKLDWPEGATSRDSLKA 380

Query: 375 VKKLDRLKDMVSQHVGFSR-LLTDLLYDLLKYDPSERPTARQALNHSFF 396
           V KL RL +++ QHV  S   L DLL  LL+YDP+ER  AR+ALNH FF
Sbjct: 381 VWKLPRLPNLIMQHVDHSAGDLIDLLQGLLRYDPTERFKAREALNHPFF 429

[Close]

BLAST of Carg08294 vs. TAIR 10
Match: AT3G53570.1 (FUS3-complementing gene 1 )

HSP 1 Score: 537.0 bits (1382), Expect = 1.3e-152
Identity = 262/409 (64.06%), Postives = 315/409 (77.02%), Query Frame = 0


Query: 15  RKRPRSAWDVVPSEQEANKALAVVK------------------------DEVKRHASPPR 74
           RKRPR  WD  P          V +                        + + R  SPP 
Sbjct: 35  RKRPRLTWDAAPPLLPPPPPPTVFQPPLYYGPEFASGLVPNFVYPNMFYNGLPRQGSPPW 94

Query: 75  RDDDREGHYVFNLGENLTPRYKILSKMGEGTFGRVLECWDRQTREYVAIKVVRSIRKYRD 134
           R DD++GHYVF +G+ LTPRY+ILSKMGEGTFG+VLEC+D + +E VAIKV+RSI KYR+
Sbjct: 95  RPDDKDGHYVFVVGDTLTPRYQILSKMGEGTFGQVLECFDNKNKEVVAIKVIRSINKYRE 154

Query: 135 AAMVEVDILKHLAQNDTGCLRCVQIRNWFDYRNHICIVFERLGPSLFDFLKRNKYHPFPV 194
           AAM+E+D+L+ L ++D G  RCVQIRNWFDYRNHICIVFE+LGPSL+DFL++N Y  FP+
Sbjct: 155 AAMIEIDVLQRLTRHDVGGSRCVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNSYRSFPI 214

Query: 195 DLVREFGRQLLESVAYMHDLHLIHTDLKPENILLVSSEYIKLPGCK---RVSSDETQFRC 254
           DLVRE GRQLLESVAYMHDL LIHTDLKPENILLVSSEYIK+P  K   R + D + F+ 
Sbjct: 215 DLVRELGRQLLESVAYMHDLRLIHTDLKPENILLVSSEYIKIPDYKFLSRPTKDGSYFKN 274

Query: 255 LPKSSAIKLIDFGSTAFDNENHSSIVSTRHYRAPEVILGLGWSYPCDLWSIGCILVELCS 314
           LPKSSAIKLIDFGST F++++H+ IVSTRHYRAPEVILG+GW+YPCDLWSIGCILVELCS
Sbjct: 275 LPKSSAIKLIDFGSTTFEHQDHNYIVSTRHYRAPEVILGVGWNYPCDLWSIGCILVELCS 334

Query: 315 GKALFQTHENLEHLAMMERVLGPIPGHMIQSADQNAEKYFKRGLRLNWPEGAVSRESIRA 374
           G+ALFQTHENLEHLAMMERVLGP+P HM+  AD+ +EKYF+RG +L+WPEGA SR+S++A
Sbjct: 335 GEALFQTHENLEHLAMMERVLGPLPPHMVLRADRRSEKYFRRGAKLDWPEGATSRDSLKA 394

Query: 375 VKKLDRLKDMVSQHVGFSR-LLTDLLYDLLKYDPSERPTARQALNHSFF 396
           V KL RL +++ QHV  S   L DLL  LL+YDP+ER  AR+ALNH FF
Sbjct: 395 VWKLPRLPNLIMQHVDHSAGDLIDLLQGLLRYDPTERFKAREALNHPFF 443

The following BLAST results are available for this feature:

BLAST of Carg08294 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023545797.1	4.9e-234	100.00	serine/threonine-protein kinase AFC3 isoform X1 [Cucurbita pepo subsp. pepo] >KA...	[more]
XP_022962075.1	1.9e-233	99.75	serine/threonine-protein kinase AFC3 isoform X1 [Cucurbita moschata]	[more]
XP_022996479.1	5.5e-233	99.50	serine/threonine-protein kinase AFC3 isoform X1 [Cucurbita maxima]	[more]
XP_038886114.1	4.2e-225	96.75	serine/threonine-protein kinase AFC3 [Benincasa hispida]	[more]
XP_022132125.1	1.2e-224	96.25	serine/threonine-protein kinase AFC3 [Momordica charantia]	[more]

BLAST of Carg08294 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P51568	1.3e-168	72.66	Serine/threonine-protein kinase AFC3 OS=Arabidopsis thaliana OX=3702 GN=AFC3 PE=...	[more]
P51566	1.9e-151	64.06	Serine/threonine-protein kinase AFC1 OS=Arabidopsis thaliana OX=3702 GN=AFC1 PE=...	[more]
P51567	9.3e-143	58.45	Serine/threonine-protein kinase AFC2 OS=Arabidopsis thaliana OX=3702 GN=AFC2 PE=...	[more]
Q10156	1.6e-94	46.25	Dual specificity protein kinase lkh1 OS=Schizosaccharomyces pombe (strain 972 / ...	[more]
O35491	7.0e-90	46.82	Dual specificity protein kinase CLK2 OS=Mus musculus OX=10090 GN=Clk2 PE=1 SV=2	[more]

BLAST of Carg08294 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HE30	9.1e-234	99.75	serine/threonine-protein kinase AFC3 isoform X1 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1K8U5	2.6e-233	99.50	serine/threonine-protein kinase AFC3 isoform X1 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1BRK0	5.9e-225	96.25	serine/threonine-protein kinase AFC3 OS=Momordica charantia OX=3673 GN=LOC111005...	[more]
A0A0A0LM05	9.4e-223	95.75	Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G375...	[more]
A0A1S3BCM1	9.4e-223	95.75	serine/threonine-protein kinase AFC3 OS=Cucumis melo OX=3656 GN=LOC103488204 PE=...	[more]

BLAST of Carg08294 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G32660.1	9.1e-170	72.66	Protein kinase superfamily protein	[more]
AT4G32660.3	1.2e-169	72.77	Protein kinase superfamily protein	[more]
AT4G32660.2	8.3e-155	73.58	Protein kinase superfamily protein	[more]
AT3G53570.3	1.3e-152	64.06	FUS3-complementing gene 1	[more]
AT3G53570.1	1.3e-152	64.06	FUS3-complementing gene 1	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 71..395 e-value: 3.1E-79 score: 279.2
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 71..395 e-value: 8.9E-52 score: 176.1
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 71..395 score: 39.679096
None	No IPR available	GENE3D	3.30.200.20	Phosphorylase Kinase; domain 1	coord: 58..151 e-value: 6.1E-113 score: 379.5
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 125..395 e-value: 6.1E-113 score: 379.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..23
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..27
None	No IPR available	PANTHER	PTHR45646	SERINE/THREONINE-PROTEIN KINASE DOA-RELATED	coord: 43..396
None	No IPR available	PANTHER	PTHR45646:SF17	BNAA07G37640D PROTEIN	coord: 43..396
None	No IPR available	CDD	cd14134	PKc_CLK	coord: 58..395 e-value: 0.0 score: 535.993
IPR008271	Serine/threonine-protein kinase, active site	PROSITE	PS00108	PROTEIN_KINASE_ST	coord: 192..204
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 58..398

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg08294-RA	Carg08294-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity