Carg08118 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg08118
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionAdenylyl-sulfate kinase
LocationCarg_Chr04: 2385942 .. 2389231 (-)
RNA-Seq ExpressionCarg08118
SyntenyCarg08118
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTTCGACTGGGGGAATTTCGTTTCCGGGATGTGTGCCGCCGCCGCTCCGCCACGAATCATTCTCGCCGCCATCGGCGTTTGGGTTTGCAAGGTTTTCCAATGGGATTAGCTTCAAATCAACGGTGGTTGTTTGTGATTGGGCTGAGAAAAAGGGTTCGAGGGCGGTGATTGTTAATGGGAAGGTTGATGGTTTGGGTAAGAGTGAATGCGAGATTGATTTAGATGCTACCTTGGGCAATGGACATGCTGGAAACTCTGGTATTGCTTCAATTTTGATGTCTGTTGTTGGTATAATCTTGATTCAGGGACTTGGTTTTGGTTGAATCAGAATATTTGTCTTGTTTTCTGAATCATCTCATTGATGTTTCTGGTGTTATGGCTGTCTTGCTTGATTCCTTAGATAGCACAAACAAGATATTGGAATAGCAAGAACCTTATGTTTGTATCTATGTCGATCTTAGCTATATTCTTATGTAGAACTCTTTGAAACGCTGATTTAGTTGCAGAACGTTTGAATTGTTTCTATGTTGATATAAGCTCATATAGTGGTGGTGTCTGATGATGTTCTTGTTTGTGTGAGATTCTTGTCTTAGTTTAAGCTGTCAAGATTACTAAGATGCTTTTCAATCAGGGAAAAATGCTGGAGTGTTGTCCACAGTAGGAAACTCTACCAATATCAAGTGGCATGAATGTTCAATTGGAAATGTTGAAAGACAGAGCCTTCTCAACCAGAAAGGATGTGTCATATGGATCACAGGGCTCAGTGGCTCAGGTTTGCTTTTACGCCTTAGTTGTTATTATCTATTAATCATCGTTTAGATAGCCTATAGTTTAGATTGTATTCGAATGAAATTAGTCTTGGAGTTTTGGGTATCCATCGATTTGGCTGGGTTTGTTCTCTGATAGATTTTTTGGGTTGTACGTGTGTTCTAATGTAACAACCTAAGCCCACCGCTAATAGATATTGTCTGCTCATGGTTTGAAAATGCGTCTACTAGGGAGAGGTTTCCACGTCCTTATAAGGAATGATTCGTTTCTCTTTCCAACTGATGTGGGATCTCACAATCCACCTCCCTTGGGGGCTAGCGTCCTCGCTGGCACACCCCCTGATGTTTGGCTCTGATACCATTTGTAACAACCCAAGCCCACCGCTAATAAATATTGTCCGCTCATGGTTTTAAAATGCATCTACTAGGGAGAGGTTTCCATGCCCTTATAAGGAATGATTTGTTCCATCTCCAACCGACGTGGGATCTCACAATCCACCTCCCTTGGGGCCAGCGTCCTCGCTAGCACACCACCTAGTGTCTGGCTCTGATATCATTTGTAATAGCCCAAGCCCACCGCTAATAGATATTGTCTGTTTTGGCTCGTTACATATAGTCGCCAGCCTCACGATTTTAAAACACGTATGCTAGGAAGGGGTTTCTATGCCCTTATAAGGAATGTTTCATTTCCCTCTGCAACCGACGTGGGATCTTACATCTAACCAAAATTATCCTTGTTCCTTCTAGATAATAGACGAAACCCAACCATGAAATATATGAGTCTCGATTGATTTTGATTTTTCAAGTTATGTTTGGAAAGATTTAGTGAAATAGATAGTAAAATATGTTGGCTTGAAATATATAAATTGGGTTTGAGACTTTTTCAACCTGACCCAATTTTAGCTAACTTGCAGGGAAGAGCTCTGTGGCTTGTGCCTTGACTAAAAGCTTATACAAAATGGGAAAGTTGGCTTATATTCTTGATGGGGACAATGTTAGGCATGGCTTGAATCGTGACCTTGGCTTTAAAGCCGAAGATCGTGCGGAGAACATAAGGAGGGTTGGTAAGTCCTGCTCGAGAAGTTTTGTTCATTTACAACACCTTGGATTGGGCTGTTCATGATTCCTAATTATACCTCTTCCATAAACTAGGTGAGGTTGCGAAACTGTTCGCAGACGCTGGAGTTATTTGTATTGCTAGTTTGATATCTCCATATCGAAGGGATCGAGATGCCTGTCGTGCCATTTTACCTGATGGATACTTTATTGAGGTATTTCTAAATTTACGCATACCATCGATTTTGAGTGGACTTCTATTCTACCTCGCCTCACCTCGCCTCGGAAACATATATGACAAAAATATACTAAAAATATACAGGTGTTCATGGATGTTCCTCTTGAAATTTGTGAAGCACGAGATACGAAGGGGCTGTATAAGCTTGCACGGGCGGGGAAGATCAAAGGTTATTGCGATTTGTTATCGTCTTACTTAGAGCTAATTGATATCAAATAGGCTTAAATATGCTCAGAATTATCATAGTTCATCCATTGACTGAACAAAATCTCATATGTTTGCAGGCTTTACCGGTATTGATGATCCATATGAAGTACCATTGAATTGTGAGGTAAAATTACACTGTCTAGTGGAAAGTGGAACATTTTAGAACATCTTGCAAAAGATAAATGCTTCTACTACTTGGCGAATGATTCTCGGGTTAAATTATAAGCTTAGTACTGATCTTTAAGTTTAGTTCTTATAGGCCCCGGGTCTTCAAAAAGTTCATAATTAATCGCTGAACTTTCAACTTTGCAACTAATAGATATCTAATTGTCTGGTAGATCAAAACTTACTCGACATTCTTTAGAATTCATTGTTAACTAAGTTGGAAGTTTAAGATTCTATTAGACACGGAATTAGAAGTCTTAAGAACGTTTTAGATATTTGAAAGTTCGATGTACTAACGCACGTGTTGGGTTTCAGATTGTACTGAAACACAATGGAGGTTCCCCATGCGACATGGCAGATAAAGTGATGAGTTTCCTTGAGCAGAAGGGCTTCCTGAAGGCTTAACTCAGAACACAATGAATAAGGGGCCTTAACAACTGAAGTTTTGGTTCAAATTTTCACTCTGTTTTAGAGCAAATCCAGTTTAACTAAAGTTTTGGCTTTGCTTCTTGTCTTTTTACGTCTAATTTGCTACTCCTTTGACCTCTTCTTTCTCTGAATACAGATAGGAATGAAGGTTTGACATAGAACGTTGTCTGGAAAAGGTTGTTGCATGTGATGATAGATCAATTCATTTTGCTATTTTGCTATTTTTTCTCTTTGGTTTGTGTGATGTAGAGTTGTTTGAAACGTGGTTGAATGTTCTATACAAATGAGTATACGATGAGTGCACGGGTCAGCTTGTGCGCACTTCGACTAATAACAGATATCGAGGATATCGAACCTATAAAGATACGTCATGCCTAATATAAACTTGTAAGTGGAGTGATTGCCATCACCACTCACTCACTCT

mRNA sequence

ATGGTTTCGACTGGGGGAATTTCGTTTCCGGGATGTGTGCCGCCGCCGCTCCGCCACGAATCATTCTCGCCGCCATCGGCGTTTGGGTTTGCAAGGTTTTCCAATGGGATTAGCTTCAAATCAACGGTGGTTGTTTGTGATTGGGCTGAGAAAAAGGGTTCGAGGGCGGTGATTGTTAATGGGAAGGTTGATGGTTTGGGTAAGAGTGAATGCGAGATTGATTTAGATGCTACCTTGGGCAATGGACATGCTGGAAACTCTGGTATTGCTTCAATTTTGATGTCTGTTGTTGGGAAAAATGCTGGAGTGTTGTCCACAGTAGGAAACTCTACCAATATCAAGTGGCATGAATGTTCAATTGGAAATGTTGAAAGACAGAGCCTTCTCAACCAGAAAGGATGTGTCATATGGATCACAGGGCTCAGTGGCTCAGGGAAGAGCTCTGTGGCTTGTGCCTTGACTAAAAGCTTATACAAAATGGGAAAGTTGGCTTATATTCTTGATGGGGACAATGTTAGGCATGGCTTGAATCGTGACCTTGGCTTTAAAGCCGAAGATCGTGCGGAGAACATAAGGAGGGTTGGTGAGGTTGCGAAACTGTTCGCAGACGCTGGAGTTATTTGTATTGCTAGTTTGATATCTCCATATCGAAGGGATCGAGATGCCTGTCGTGCCATTTTACCTGATGGATACTTTATTGAGGTGTTCATGGATGTTCCTCTTGAAATTTGTGAAGCACGAGATACGAAGGGGCTGTATAAGCTTGCACGGGCGGGGAAGATCAAAGGCTTTACCGGTATTGATGATCCATATGAAGTACCATTGAATTGTGAGATTGTACTGAAACACAATGGAGGTTCCCCATGCGACATGGCAGATAAAGTGATGAGTTTCCTTGAGCAGAAGGGCTTCCTGAAGGCTTAACTCAGAACACAATGAATAAGGGGCCTTAACAACTGAAGTTTTGGTTCAAATTTTCACTCTGTTTTAGAGCAAATCCAGTTTAACTAAAGTTTTGGCTTTGCTTCTTGTCTTTTTACGTCTAATTTGCTACTCCTTTGACCTCTTCTTTCTCTGAATACAGATAGGAATGAAGGTTTGACATAGAACGTTGTCTGGAAAAGGTTGTTGCATGTGATGATAGATCAATTCATTTTGCTATTTTGCTATTTTTTCTCTTTGGTTTGTGTGATGTAGAGTTGTTTGAAACGTGGTTGAATGTTCTATACAAATGAGTATACGATGAGTGCACGGGTCAGCTTGTGCGCACTTCGACTAATAACAGATATCGAGGATATCGAACCTATAAAGATACGTCATGCCTAATATAAACTTGTAAGTGGAGTGATTGCCATCACCACTCACTCACTCT

Coding sequence (CDS)

ATGGTTTCGACTGGGGGAATTTCGTTTCCGGGATGTGTGCCGCCGCCGCTCCGCCACGAATCATTCTCGCCGCCATCGGCGTTTGGGTTTGCAAGGTTTTCCAATGGGATTAGCTTCAAATCAACGGTGGTTGTTTGTGATTGGGCTGAGAAAAAGGGTTCGAGGGCGGTGATTGTTAATGGGAAGGTTGATGGTTTGGGTAAGAGTGAATGCGAGATTGATTTAGATGCTACCTTGGGCAATGGACATGCTGGAAACTCTGGTATTGCTTCAATTTTGATGTCTGTTGTTGGGAAAAATGCTGGAGTGTTGTCCACAGTAGGAAACTCTACCAATATCAAGTGGCATGAATGTTCAATTGGAAATGTTGAAAGACAGAGCCTTCTCAACCAGAAAGGATGTGTCATATGGATCACAGGGCTCAGTGGCTCAGGGAAGAGCTCTGTGGCTTGTGCCTTGACTAAAAGCTTATACAAAATGGGAAAGTTGGCTTATATTCTTGATGGGGACAATGTTAGGCATGGCTTGAATCGTGACCTTGGCTTTAAAGCCGAAGATCGTGCGGAGAACATAAGGAGGGTTGGTGAGGTTGCGAAACTGTTCGCAGACGCTGGAGTTATTTGTATTGCTAGTTTGATATCTCCATATCGAAGGGATCGAGATGCCTGTCGTGCCATTTTACCTGATGGATACTTTATTGAGGTGTTCATGGATGTTCCTCTTGAAATTTGTGAAGCACGAGATACGAAGGGGCTGTATAAGCTTGCACGGGCGGGGAAGATCAAAGGCTTTACCGGTATTGATGATCCATATGAAGTACCATTGAATTGTGAGATTGTACTGAAACACAATGGAGGTTCCCCATGCGACATGGCAGATAAAGTGATGAGTTTCCTTGAGCAGAAGGGCTTCCTGAAGGCTTAA

Protein sequence

MVSTGGISFPGCVPPPLRHESFSPPSAFGFARFSNGISFKSTVVVCDWAEKKGSRAVIVNGKVDGLGKSECEIDLDATLGNGHAGNSGIASILMSVVGKNAGVLSTVGNSTNIKWHECSIGNVERQSLLNQKGCVIWITGLSGSGKSSVACALTKSLYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENIRRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVPLEICEARDTKGLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHNGGSPCDMADKVMSFLEQKGFLKA
Homology
BLAST of Carg08118 vs. NCBI nr
Match: KAG7030942.1 (Adenylyl-sulfate kinase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 627.9 bits (1618), Expect = 4.7e-176
Identity = 307/307 (100.00%), Postives = 307/307 (100.00%), Query Frame = 0

Query: 1   MVSTGGISFPGCVPPPLRHESFSPPSAFGFARFSNGISFKSTVVVCDWAEKKGSRAVIVN 60
           MVSTGGISFPGCVPPPLRHESFSPPSAFGFARFSNGISFKSTVVVCDWAEKKGSRAVIVN
Sbjct: 1   MVSTGGISFPGCVPPPLRHESFSPPSAFGFARFSNGISFKSTVVVCDWAEKKGSRAVIVN 60

Query: 61  GKVDGLGKSECEIDLDATLGNGHAGNSGIASILMSVVGKNAGVLSTVGNSTNIKWHECSI 120
           GKVDGLGKSECEIDLDATLGNGHAGNSGIASILMSVVGKNAGVLSTVGNSTNIKWHECSI
Sbjct: 61  GKVDGLGKSECEIDLDATLGNGHAGNSGIASILMSVVGKNAGVLSTVGNSTNIKWHECSI 120

Query: 121 GNVERQSLLNQKGCVIWITGLSGSGKSSVACALTKSLYKMGKLAYILDGDNVRHGLNRDL 180
           GNVERQSLLNQKGCVIWITGLSGSGKSSVACALTKSLYKMGKLAYILDGDNVRHGLNRDL
Sbjct: 121 GNVERQSLLNQKGCVIWITGLSGSGKSSVACALTKSLYKMGKLAYILDGDNVRHGLNRDL 180

Query: 181 GFKAEDRAENIRRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVP 240
           GFKAEDRAENIRRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVP
Sbjct: 181 GFKAEDRAENIRRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVP 240

Query: 241 LEICEARDTKGLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHNGGSPCDMADKVMSFLE 300
           LEICEARDTKGLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHNGGSPCDMADKVMSFLE
Sbjct: 241 LEICEARDTKGLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHNGGSPCDMADKVMSFLE 300

Query: 301 QKGFLKA 308
           QKGFLKA
Sbjct: 301 QKGFLKA 307

BLAST of Carg08118 vs. NCBI nr
Match: XP_022984565.1 (adenylyl-sulfate kinase 3-like [Cucurbita maxima])

HSP 1 Score: 601.3 bits (1549), Expect = 4.7e-168
Identity = 295/307 (96.09%), Postives = 297/307 (96.74%), Query Frame = 0

Query: 1   MVSTGGISFPGCVPPPLRHESFSPPSAFGFARFSNGISFKSTVVVCDWAEKKGSRAVIVN 60
           MVSTGGISFPGCVPPPLRH+SFSPPSAFGFARFSNGISFKSTVVVCDWAEKKGSRAVIVN
Sbjct: 1   MVSTGGISFPGCVPPPLRHKSFSPPSAFGFARFSNGISFKSTVVVCDWAEKKGSRAVIVN 60

Query: 61  GKVDGLGKSECEIDLDATLGNGHAGNSGIASILMSVVGKNAGVLSTVGNSTNIKWHECSI 120
           GKVDGLGKSECEIDLDATLGNGHAGNS          GKNAGVLSTVGNSTNIKWHECSI
Sbjct: 61  GKVDGLGKSECEIDLDATLGNGHAGNS----------GKNAGVLSTVGNSTNIKWHECSI 120

Query: 121 GNVERQSLLNQKGCVIWITGLSGSGKSSVACALTKSLYKMGKLAYILDGDNVRHGLNRDL 180
           GNVERQSLLNQKGCVIWITGLSGSGKSSVACALTKSLYKMGKLAYILDGDNVRHGLNRDL
Sbjct: 121 GNVERQSLLNQKGCVIWITGLSGSGKSSVACALTKSLYKMGKLAYILDGDNVRHGLNRDL 180

Query: 181 GFKAEDRAENIRRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVP 240
           GFKAEDRAENIRRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVP
Sbjct: 181 GFKAEDRAENIRRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVP 240

Query: 241 LEICEARDTKGLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHNGGSPCDMADKVMSFLE 300
           LEICEARDTKGLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHNGGSPCDMADKVMS+LE
Sbjct: 241 LEICEARDTKGLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHNGGSPCDMADKVMSYLE 297

Query: 301 QKGFLKA 308
           QKGFLKA
Sbjct: 301 QKGFLKA 297

BLAST of Carg08118 vs. NCBI nr
Match: XP_022943061.1 (adenylyl-sulfate kinase 3-like [Cucurbita moschata])

HSP 1 Score: 597.0 bits (1538), Expect = 8.9e-167
Identity = 294/307 (95.77%), Postives = 295/307 (96.09%), Query Frame = 0

Query: 1   MVSTGGISFPGCVPPPLRHESFSPPSAFGFARFSNGISFKSTVVVCDWAEKKGSRAVIVN 60
           MVSTGGISFPGCVPPPLR +SFSPPSAFGFAR SNGISFKSTVVVCDWAEKKGSRAVIVN
Sbjct: 1   MVSTGGISFPGCVPPPLRQKSFSPPSAFGFARLSNGISFKSTVVVCDWAEKKGSRAVIVN 60

Query: 61  GKVDGLGKSECEIDLDATLGNGHAGNSGIASILMSVVGKNAGVLSTVGNSTNIKWHECSI 120
           GKVDGLGKSECEIDLDATLGNGHAGNS          GKNAGVLSTVGNSTNIKWHECSI
Sbjct: 61  GKVDGLGKSECEIDLDATLGNGHAGNS----------GKNAGVLSTVGNSTNIKWHECSI 120

Query: 121 GNVERQSLLNQKGCVIWITGLSGSGKSSVACALTKSLYKMGKLAYILDGDNVRHGLNRDL 180
           GNVERQSLLNQKGCVIWITGLSGSGKSSVACALTKSLYKMGKLAYILDGDNVRHGLNRDL
Sbjct: 121 GNVERQSLLNQKGCVIWITGLSGSGKSSVACALTKSLYKMGKLAYILDGDNVRHGLNRDL 180

Query: 181 GFKAEDRAENIRRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVP 240
           GFKAEDRAENIRRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVP
Sbjct: 181 GFKAEDRAENIRRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVP 240

Query: 241 LEICEARDTKGLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHNGGSPCDMADKVMSFLE 300
           LEICEARDTKGLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHNGGSPCDMADKVMSFLE
Sbjct: 241 LEICEARDTKGLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHNGGSPCDMADKVMSFLE 297

Query: 301 QKGFLKA 308
           QKGFLKA
Sbjct: 301 QKGFLKA 297

BLAST of Carg08118 vs. NCBI nr
Match: XP_023515167.1 (adenylyl-sulfate kinase 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 589.3 bits (1518), Expect = 1.9e-164
Identity = 293/308 (95.13%), Postives = 295/308 (95.78%), Query Frame = 0

Query: 1   MVSTGGISFPGCV-PPPLRHESFSPPSAFGFARFSNGISFKSTVVVCDWAEKKGSRAVIV 60
           MVSTGGISFPGCV PPPLRH+SFSPPSAFGFARFSNGISFKS VVVCD AEKKGSRAVIV
Sbjct: 1   MVSTGGISFPGCVPPPPLRHKSFSPPSAFGFARFSNGISFKSMVVVCDGAEKKGSRAVIV 60

Query: 61  NGKVDGLGKSECEIDLDATLGNGHAGNSGIASILMSVVGKNAGVLSTVGNSTNIKWHECS 120
           NGKVDGLGKSECEIDLDATLGNGHAGNS          GKNAGVLSTVGNSTNIKWHECS
Sbjct: 61  NGKVDGLGKSECEIDLDATLGNGHAGNS----------GKNAGVLSTVGNSTNIKWHECS 120

Query: 121 IGNVERQSLLNQKGCVIWITGLSGSGKSSVACALTKSLYKMGKLAYILDGDNVRHGLNRD 180
           IGNVERQSLLNQKGCVIWITGLSGSGKSSVACALTKSLYKMGKLAYILDGDNVRHGLNRD
Sbjct: 121 IGNVERQSLLNQKGCVIWITGLSGSGKSSVACALTKSLYKMGKLAYILDGDNVRHGLNRD 180

Query: 181 LGFKAEDRAENIRRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDV 240
           LGFKAEDRAENIRRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDV
Sbjct: 181 LGFKAEDRAENIRRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDV 240

Query: 241 PLEICEARDTKGLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHNGGSPCDMADKVMSFL 300
           PLEICEARDTKGLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHNGGSPCDMADKVMS+L
Sbjct: 241 PLEICEARDTKGLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHNGGSPCDMADKVMSYL 298

Query: 301 EQKGFLKA 308
           EQKGFLKA
Sbjct: 301 EQKGFLKA 298

BLAST of Carg08118 vs. NCBI nr
Match: KAG6600284.1 (Endo-1,4-beta-xylanase 5-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 546.6 bits (1407), Expect = 1.4e-151
Identity = 269/285 (94.39%), Postives = 272/285 (95.44%), Query Frame = 0

Query: 1   MVSTGGISFPGCVPPPLRHESFSPPSAFGFARFSNGISFKSTVVVCDWAEKKGSRAVIVN 60
           MVSTGGISFPGCVPPPLRH+SFSPPSAFGFARFSNGISFKSTVVVCDWAEKKGSRAVIVN
Sbjct: 1   MVSTGGISFPGCVPPPLRHKSFSPPSAFGFARFSNGISFKSTVVVCDWAEKKGSRAVIVN 60

Query: 61  GKVDGLGKSECEIDLDATLGNGHAGNSGIASILMSVVGKNAGVLSTVGNSTNIKWHECSI 120
           GKVDGLGKSECEIDLDATLGNGHAGNS          GKNAGVLSTVGNSTNIKWHECSI
Sbjct: 61  GKVDGLGKSECEIDLDATLGNGHAGNS----------GKNAGVLSTVGNSTNIKWHECSI 120

Query: 121 GNVERQSLLNQKGCVIWITGLSGSGKSSVACALTKSLYKMGKLAYILDGDNVRHGLNRDL 180
           GNVERQSLLNQKGCVIWITGLSGSGKSSVACALTKSLYKMGKLAYILDGDNVRHGLNRDL
Sbjct: 121 GNVERQSLLNQKGCVIWITGLSGSGKSSVACALTKSLYKMGKLAYILDGDNVRHGLNRDL 180

Query: 181 GFKAEDRAENIRRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVP 240
           GFKAEDRAENIRRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVP
Sbjct: 181 GFKAEDRAENIRRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVP 240

Query: 241 LEICEARDTKGLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHNG 286
           LEICEARDTKGLYKLARAGKIKGFTGIDDPYEVPLNCE ++K  G
Sbjct: 241 LEICEARDTKGLYKLARAGKIKGFTGIDDPYEVPLNCECLVKPRG 275

BLAST of Carg08118 vs. ExPASy Swiss-Prot
Match: Q43295 (Adenylyl-sulfate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APK1 PE=1 SV=1)

HSP 1 Score: 353.6 bits (906), Expect = 2.3e-96
Identity = 177/273 (64.84%), Postives = 209/273 (76.56%), Query Frame = 0

Query: 36  GISFKSTVVVCDWAEKKGSRAVIVNGKVDGLGKSECEIDLDATLGNGHAGNSGIASILMS 95
           G+S  S   + D      SR+V+V        ++   +D   TL +   G+      +  
Sbjct: 11  GLSMASPKGIFDSNSMSNSRSVVV-------VRACVSMDGSQTLSHNKNGSIPEVKSING 70

Query: 96  VVGKNAGVLSTVGNSTNIKWHECSIGNVERQSLLNQKGCVIWITGLSGSGKSSVACALTK 155
             G+  G LSTVGNSTNIKWHECS+  V+RQ LL+QKGCVIW+TGLSGSGKS++ACAL +
Sbjct: 71  HTGQKQGPLSTVGNSTNIKWHECSVEKVDRQRLLDQKGCVIWVTGLSGSGKSTLACALNQ 130

Query: 156 SLYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENIRRVGEVAKLFADAGVICIASLISP 215
            LY+ GKL YILDGDNVRHGLNRDL FKAEDRAENIRRVGEVAKLFADAG+ICIASLISP
Sbjct: 131 MLYQKGKLCYILDGDNVRHGLNRDLSFKAEDRAENIRRVGEVAKLFADAGIICIASLISP 190

Query: 216 YRRDRDACRAILPDGYFIEVFMDVPLEICEARDTKGLYKLARAGKIKGFTGIDDPYEVPL 275
           YR DRDACR++LP+G F+EVFMDVPL +CEARD KGLYKLARAGKIKGFTGIDDPYE PL
Sbjct: 191 YRTDRDACRSLLPEGDFVEVFMDVPLSVCEARDPKGLYKLARAGKIKGFTGIDDPYEPPL 250

Query: 276 NCEIVLKHNGG-SPCDMADKVMSFLEQKGFLKA 308
           NCEI L   GG SP +MA+KV+ +L+ KG+L+A
Sbjct: 251 NCEISLGREGGTSPIEMAEKVVGYLDNKGYLQA 276

BLAST of Carg08118 vs. ExPASy Swiss-Prot
Match: O49204 (Adenylyl-sulfate kinase, chloroplastic OS=Catharanthus roseus OX=4058 GN=AKN PE=2 SV=1)

HSP 1 Score: 332.8 bits (852), Expect = 4.1e-90
Identity = 169/255 (66.27%), Postives = 196/255 (76.86%), Query Frame = 0

Query: 59  VNGKVDG---LGKSECEIDLDATLGNGHAGNSGIASILMSVVGKNAGVLSTVGNSTNIKW 118
           VNGKVD    L  S+C  + D++L N +              GK     +TVGNSTNI W
Sbjct: 69  VNGKVDNFRHLQPSDCNSNSDSSLNNCN-----------GFPGKKILQTTTVGNSTNILW 128

Query: 119 HECSIGNVERQSLLNQKGCVIWITGLSGSGKSSVACALTKSLYKMGKLAYILDGDNVRHG 178
           H+C++   ERQ  L Q+GCVIWITGLSGSGKS++ACAL++ L+  GKL YILDGDNVRHG
Sbjct: 129 HKCAVEKSERQEPLQQRGCVIWITGLSGSGKSTLACALSRGLHAKGKLTYILDGDNVRHG 188

Query: 179 LNRDLGFKAEDRAENIRRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEV 238
           LN DL FKAEDRAENIRR+GEVAKLFADAGVICIASLISPYR+  DACR++LP+G FIEV
Sbjct: 189 LNSDLSFKAEDRAENIRRIGEVAKLFADAGVICIASLISPYRKPPDACRSLLPEGDFIEV 248

Query: 239 FMDVPLEICEARDTKGLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHNGG---SPCDMA 298
           FMDVPL++CEARD KGLYKLARAGKIKGFTGIDDPYE PL  EIVL    G   SPCD+A
Sbjct: 249 FMDVPLKVCEARDPKGLYKLARAGKIKGFTGIDDPYEPPLKSEIVLHQKLGMCDSPCDLA 308

Query: 299 DKVMSFLEQKGFLKA 308
           D V+S+LE+ G+LKA
Sbjct: 309 DIVISYLEENGYLKA 312

BLAST of Carg08118 vs. ExPASy Swiss-Prot
Match: O49196 (Adenylyl-sulfate kinase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APK2 PE=1 SV=1)

HSP 1 Score: 320.9 bits (821), Expect = 1.6e-86
Identity = 149/199 (74.87%), Postives = 171/199 (85.93%), Query Frame = 0

Query: 112 NIKWHECSIGNVERQSLLNQKGCVIWITGLSGSGKSSVACALTKSLYKMGKLAYILDGDN 171
           NI WHE SI   +RQ LL QKGCV+WITGLSGSGKS+VACAL+K+L++ GKL Y LDGDN
Sbjct: 94  NIVWHESSICRCDRQQLLQQKGCVVWITGLSGSGKSTVACALSKALFERGKLTYTLDGDN 153

Query: 172 VRHGLNRDLGFKAEDRAENIRRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGY 231
           VRHGLNRDL FKAE R ENIRR+GEVAKLFAD GVICIASLISPYRRDRDACR++LPDG 
Sbjct: 154 VRHGLNRDLTFKAEHRTENIRRIGEVAKLFADVGVICIASLISPYRRDRDACRSLLPDGD 213

Query: 232 FIEVFMDVPLEICEARDTKGLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHNGG----S 291
           F+EVFMDVPL +CE+RD KGLYKLARAGKIKGFTGIDDPYE P+NCE+VLKH G     S
Sbjct: 214 FVEVFMDVPLHVCESRDPKGLYKLARAGKIKGFTGIDDPYEAPVNCEVVLKHTGDDESCS 273

Query: 292 PCDMADKVMSFLEQKGFLK 307
           P  MA+ ++S+L+ KG+L+
Sbjct: 274 PRQMAENIISYLQNKGYLE 292

BLAST of Carg08118 vs. ExPASy Swiss-Prot
Match: Q9SRW7 (Adenylyl-sulfate kinase 3 OS=Arabidopsis thaliana OX=3702 GN=APK3 PE=1 SV=1)

HSP 1 Score: 320.9 bits (821), Expect = 1.6e-86
Identity = 153/206 (74.27%), Postives = 177/206 (85.92%), Query Frame = 0

Query: 104 LSTVGNSTNIKWHECSIGNVERQSLLNQKGCVIWITGLSGSGKSSVACALTKSLYKMGKL 163
           +STVGNSTNI W E  IG  ERQ LLNQKGCV+WITGLSGSGKS++AC+L++ L   GKL
Sbjct: 1   MSTVGNSTNIFWQESPIGKTERQKLLNQKGCVVWITGLSGSGKSTLACSLSRELNNRGKL 60

Query: 164 AYILDGDNVRHGLNRDLGFKAEDRAENIRRVGEVAKLFADAGVICIASLISPYRRDRDAC 223
           +YILDGDN+RHGLN+DLGFKAEDR ENIRRVGEVAKLFADAG+ICIASLISPYR+DRDAC
Sbjct: 61  SYILDGDNLRHGLNKDLGFKAEDRVENIRRVGEVAKLFADAGLICIASLISPYRKDRDAC 120

Query: 224 RAILPDGYFIEVFMDVPLEICEARDTKGLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKH 283
           R ++ +  FIEVFM++ L++CEARD KGLYKLARAGKIKGFTGIDDPYE PLNCEI LK 
Sbjct: 121 REMIQNSSFIEVFMNMSLQLCEARDPKGLYKLARAGKIKGFTGIDDPYESPLNCEIELKE 180

Query: 284 NGG---SPCDMADKVMSFLEQKGFLK 307
             G   SP  MA++V+S+LE KGFL+
Sbjct: 181 KEGECPSPVAMAEEVISYLEDKGFLQ 206

BLAST of Carg08118 vs. ExPASy Swiss-Prot
Match: Q84JF0 (Adenylyl-sulfate kinase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APK4 PE=1 SV=1)

HSP 1 Score: 304.7 bits (779), Expect = 1.2e-81
Identity = 144/217 (66.36%), Postives = 172/217 (79.26%), Query Frame = 0

Query: 108 GNSTNIKWHECSIGNVERQSLLNQKGCVIWITGLSGSGKSSVACALTKSLYKMGKLAYIL 167
           G   NI WH+C +   +RQ L+ QKGCVIWITGLSGSGKSS+ACAL+++L+  GKL+YIL
Sbjct: 84  GKQKNIVWHDCPVTKSDRQELIKQKGCVIWITGLSGSGKSSLACALSRALHNRGKLSYIL 143

Query: 168 DGDNVRHGLNRDLGFKAEDRAENIRRVGEVAKLFADAGVICIASLISPYRRDRDACRAIL 227
           DGDNVRHGLN DL F+A+DRAENIRRVGEVAKLFAD+G+ICIASLISPYR +R ACRA+L
Sbjct: 144 DGDNVRHGLNSDLSFEADDRAENIRRVGEVAKLFADSGIICIASLISPYRIERAACRALL 203

Query: 228 PDGYFIEVFMDVPLEICEARDTKGLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKH---- 287
           P G FIEVFMDVPL +CEARD KGLYK ARAGKIKGFTG+DDPYE PL+CEIV+++    
Sbjct: 204 PQGDFIEVFMDVPLHVCEARDPKGLYKRARAGKIKGFTGVDDPYEAPLDCEIVIQNSRDK 263

Query: 288 --------------NGGSPCDMADKVMSFLEQKGFLK 307
                         +  S C+MAD V+S+L+Q G+LK
Sbjct: 264 GLSSSSSSSSSPSSSSSSLCEMADIVVSYLDQNGYLK 300

BLAST of Carg08118 vs. ExPASy TrEMBL
Match: A0A6J1J8X7 (Adenylyl-sulfate kinase OS=Cucurbita maxima OX=3661 GN=LOC111482821 PE=3 SV=1)

HSP 1 Score: 601.3 bits (1549), Expect = 2.3e-168
Identity = 295/307 (96.09%), Postives = 297/307 (96.74%), Query Frame = 0

Query: 1   MVSTGGISFPGCVPPPLRHESFSPPSAFGFARFSNGISFKSTVVVCDWAEKKGSRAVIVN 60
           MVSTGGISFPGCVPPPLRH+SFSPPSAFGFARFSNGISFKSTVVVCDWAEKKGSRAVIVN
Sbjct: 1   MVSTGGISFPGCVPPPLRHKSFSPPSAFGFARFSNGISFKSTVVVCDWAEKKGSRAVIVN 60

Query: 61  GKVDGLGKSECEIDLDATLGNGHAGNSGIASILMSVVGKNAGVLSTVGNSTNIKWHECSI 120
           GKVDGLGKSECEIDLDATLGNGHAGNS          GKNAGVLSTVGNSTNIKWHECSI
Sbjct: 61  GKVDGLGKSECEIDLDATLGNGHAGNS----------GKNAGVLSTVGNSTNIKWHECSI 120

Query: 121 GNVERQSLLNQKGCVIWITGLSGSGKSSVACALTKSLYKMGKLAYILDGDNVRHGLNRDL 180
           GNVERQSLLNQKGCVIWITGLSGSGKSSVACALTKSLYKMGKLAYILDGDNVRHGLNRDL
Sbjct: 121 GNVERQSLLNQKGCVIWITGLSGSGKSSVACALTKSLYKMGKLAYILDGDNVRHGLNRDL 180

Query: 181 GFKAEDRAENIRRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVP 240
           GFKAEDRAENIRRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVP
Sbjct: 181 GFKAEDRAENIRRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVP 240

Query: 241 LEICEARDTKGLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHNGGSPCDMADKVMSFLE 300
           LEICEARDTKGLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHNGGSPCDMADKVMS+LE
Sbjct: 241 LEICEARDTKGLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHNGGSPCDMADKVMSYLE 297

Query: 301 QKGFLKA 308
           QKGFLKA
Sbjct: 301 QKGFLKA 297

BLAST of Carg08118 vs. ExPASy TrEMBL
Match: A0A6J1FQP4 (Adenylyl-sulfate kinase OS=Cucurbita moschata OX=3662 GN=LOC111447912 PE=3 SV=1)

HSP 1 Score: 597.0 bits (1538), Expect = 4.3e-167
Identity = 294/307 (95.77%), Postives = 295/307 (96.09%), Query Frame = 0

Query: 1   MVSTGGISFPGCVPPPLRHESFSPPSAFGFARFSNGISFKSTVVVCDWAEKKGSRAVIVN 60
           MVSTGGISFPGCVPPPLR +SFSPPSAFGFAR SNGISFKSTVVVCDWAEKKGSRAVIVN
Sbjct: 1   MVSTGGISFPGCVPPPLRQKSFSPPSAFGFARLSNGISFKSTVVVCDWAEKKGSRAVIVN 60

Query: 61  GKVDGLGKSECEIDLDATLGNGHAGNSGIASILMSVVGKNAGVLSTVGNSTNIKWHECSI 120
           GKVDGLGKSECEIDLDATLGNGHAGNS          GKNAGVLSTVGNSTNIKWHECSI
Sbjct: 61  GKVDGLGKSECEIDLDATLGNGHAGNS----------GKNAGVLSTVGNSTNIKWHECSI 120

Query: 121 GNVERQSLLNQKGCVIWITGLSGSGKSSVACALTKSLYKMGKLAYILDGDNVRHGLNRDL 180
           GNVERQSLLNQKGCVIWITGLSGSGKSSVACALTKSLYKMGKLAYILDGDNVRHGLNRDL
Sbjct: 121 GNVERQSLLNQKGCVIWITGLSGSGKSSVACALTKSLYKMGKLAYILDGDNVRHGLNRDL 180

Query: 181 GFKAEDRAENIRRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVP 240
           GFKAEDRAENIRRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVP
Sbjct: 181 GFKAEDRAENIRRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVP 240

Query: 241 LEICEARDTKGLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHNGGSPCDMADKVMSFLE 300
           LEICEARDTKGLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHNGGSPCDMADKVMSFLE
Sbjct: 241 LEICEARDTKGLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHNGGSPCDMADKVMSFLE 297

Query: 301 QKGFLKA 308
           QKGFLKA
Sbjct: 301 QKGFLKA 297

BLAST of Carg08118 vs. ExPASy TrEMBL
Match: A0A1S3BYH9 (Adenylyl-sulfate kinase OS=Cucumis melo OX=3656 GN=LOC103494812 PE=3 SV=1)

HSP 1 Score: 502.7 bits (1293), Expect = 1.1e-138
Identity = 250/307 (81.43%), Postives = 268/307 (87.30%), Query Frame = 0

Query: 1   MVSTGGISFPGCVPPPLRHESFSPPSAFGFARFSNGISFKSTVVVCDWAEKKGSRAVIVN 60
           MVS G IS PG VPP L H+  SPPSA GFARF + ISF+S+VV+ D  EKKGSR VIVN
Sbjct: 1   MVSIGRISLPGSVPPSLHHQC-SPPSAIGFARFPDRISFRSSVVLRDEVEKKGSRVVIVN 60

Query: 61  GKVDGLGKSECEIDLDATLGNGHAGNSGIASILMSVVGKNAGVLSTVGNSTNIKWHECSI 120
           GKVDGLGKSECE + D  LGNG AGNS          GKN GVLSTVGNSTNIKWHECS+
Sbjct: 61  GKVDGLGKSECETEFDTALGNGRAGNS----------GKNHGVLSTVGNSTNIKWHECSV 120

Query: 121 GNVERQSLLNQKGCVIWITGLSGSGKSSVACALTKSLYKMGKLAYILDGDNVRHGLNRDL 180
           G  E+QSLL QKGCVIW TGLSGSGKS+VACAL++SL KMGKLAYILDGDNVRHGLNRDL
Sbjct: 121 GKNEKQSLLKQKGCVIWFTGLSGSGKSTVACALSQSLCKMGKLAYILDGDNVRHGLNRDL 180

Query: 181 GFKAEDRAENIRRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVP 240
           GFKAEDRAENIRRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVP
Sbjct: 181 GFKAEDRAENIRRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVP 240

Query: 241 LEICEARDTKGLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHNGGSPCDMADKVMSFLE 300
           LE+CEARD KGLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKH GGSP +MA+KV+++LE
Sbjct: 241 LEVCEARDAKGLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHTGGSPSEMAEKVLNYLE 296

Query: 301 QKGFLKA 308
           QKGFL+A
Sbjct: 301 QKGFLQA 296

BLAST of Carg08118 vs. ExPASy TrEMBL
Match: A0A5A7TN80 (Adenylyl-sulfate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G00500 PE=3 SV=1)

HSP 1 Score: 502.7 bits (1293), Expect = 1.1e-138
Identity = 250/307 (81.43%), Postives = 268/307 (87.30%), Query Frame = 0

Query: 1   MVSTGGISFPGCVPPPLRHESFSPPSAFGFARFSNGISFKSTVVVCDWAEKKGSRAVIVN 60
           MVS G IS PG VPP L H+  SPPSA GFARF + ISF+S+VV+ D  EKKGSR VIVN
Sbjct: 1   MVSIGRISLPGSVPPSLHHQC-SPPSAIGFARFPDRISFRSSVVLRDEVEKKGSRVVIVN 60

Query: 61  GKVDGLGKSECEIDLDATLGNGHAGNSGIASILMSVVGKNAGVLSTVGNSTNIKWHECSI 120
           GKVDGLGKSECE + D  LGNG AGNS          GKN GVLSTVGNSTNIKWHECS+
Sbjct: 61  GKVDGLGKSECETEFDTALGNGRAGNS----------GKNHGVLSTVGNSTNIKWHECSV 120

Query: 121 GNVERQSLLNQKGCVIWITGLSGSGKSSVACALTKSLYKMGKLAYILDGDNVRHGLNRDL 180
           G  E+QSLL QKGCVIW TGLSGSGKS+VACAL++SL KMGKLAYILDGDNVRHGLNRDL
Sbjct: 121 GKNEKQSLLKQKGCVIWFTGLSGSGKSTVACALSQSLCKMGKLAYILDGDNVRHGLNRDL 180

Query: 181 GFKAEDRAENIRRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVP 240
           GFKAEDRAENIRRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVP
Sbjct: 181 GFKAEDRAENIRRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVP 240

Query: 241 LEICEARDTKGLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHNGGSPCDMADKVMSFLE 300
           LE+CEARD KGLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKH GGSP +MA+KV+++LE
Sbjct: 241 LEVCEARDAKGLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHTGGSPSEMAEKVLNYLE 296

Query: 301 QKGFLKA 308
           QKGFL+A
Sbjct: 301 QKGFLQA 296

BLAST of Carg08118 vs. ExPASy TrEMBL
Match: A0A5D3DZZ6 (Adenylyl-sulfate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G00510 PE=3 SV=1)

HSP 1 Score: 501.1 bits (1289), Expect = 3.2e-138
Identity = 249/306 (81.37%), Postives = 267/306 (87.25%), Query Frame = 0

Query: 1   MVSTGGISFPGCVPPPLRHESFSPPSAFGFARFSNGISFKSTVVVCDWAEKKGSRAVIVN 60
           MVS G IS PG VPP L H+  SPPSA GFARF + ISF+S+VV+ D  EKKGSR VIVN
Sbjct: 1   MVSIGRISLPGSVPPSLHHQC-SPPSAIGFARFPDRISFRSSVVLRDEVEKKGSRVVIVN 60

Query: 61  GKVDGLGKSECEIDLDATLGNGHAGNSGIASILMSVVGKNAGVLSTVGNSTNIKWHECSI 120
           GKVDGLGKSECE + D  LGNG AGNS          GKN GVLSTVGNSTNIKWHECS+
Sbjct: 61  GKVDGLGKSECETEFDTALGNGRAGNS----------GKNHGVLSTVGNSTNIKWHECSV 120

Query: 121 GNVERQSLLNQKGCVIWITGLSGSGKSSVACALTKSLYKMGKLAYILDGDNVRHGLNRDL 180
           G  E+QSLL QKGCVIW TGLSGSGKS+VACAL++SL KMGKLAYILDGDNVRHGLNRDL
Sbjct: 121 GKNEKQSLLKQKGCVIWFTGLSGSGKSTVACALSQSLCKMGKLAYILDGDNVRHGLNRDL 180

Query: 181 GFKAEDRAENIRRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVP 240
           GFKAEDRAENIRRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVP
Sbjct: 181 GFKAEDRAENIRRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVFMDVP 240

Query: 241 LEICEARDTKGLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHNGGSPCDMADKVMSFLE 300
           LE+CEARD KGLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKH GGSP +MA+KV+++LE
Sbjct: 241 LEVCEARDAKGLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHTGGSPSEMAEKVLNYLE 295

Query: 301 QKGFLK 307
           QKGFL+
Sbjct: 301 QKGFLQ 295

BLAST of Carg08118 vs. TAIR 10
Match: AT2G14750.1 (APS kinase )

HSP 1 Score: 353.6 bits (906), Expect = 1.6e-97
Identity = 177/273 (64.84%), Postives = 209/273 (76.56%), Query Frame = 0

Query: 36  GISFKSTVVVCDWAEKKGSRAVIVNGKVDGLGKSECEIDLDATLGNGHAGNSGIASILMS 95
           G+S  S   + D      SR+V+V        ++   +D   TL +   G+      +  
Sbjct: 11  GLSMASPKGIFDSNSMSNSRSVVV-------VRACVSMDGSQTLSHNKNGSIPEVKSING 70

Query: 96  VVGKNAGVLSTVGNSTNIKWHECSIGNVERQSLLNQKGCVIWITGLSGSGKSSVACALTK 155
             G+  G LSTVGNSTNIKWHECS+  V+RQ LL+QKGCVIW+TGLSGSGKS++ACAL +
Sbjct: 71  HTGQKQGPLSTVGNSTNIKWHECSVEKVDRQRLLDQKGCVIWVTGLSGSGKSTLACALNQ 130

Query: 156 SLYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENIRRVGEVAKLFADAGVICIASLISP 215
            LY+ GKL YILDGDNVRHGLNRDL FKAEDRAENIRRVGEVAKLFADAG+ICIASLISP
Sbjct: 131 MLYQKGKLCYILDGDNVRHGLNRDLSFKAEDRAENIRRVGEVAKLFADAGIICIASLISP 190

Query: 216 YRRDRDACRAILPDGYFIEVFMDVPLEICEARDTKGLYKLARAGKIKGFTGIDDPYEVPL 275
           YR DRDACR++LP+G F+EVFMDVPL +CEARD KGLYKLARAGKIKGFTGIDDPYE PL
Sbjct: 191 YRTDRDACRSLLPEGDFVEVFMDVPLSVCEARDPKGLYKLARAGKIKGFTGIDDPYEPPL 250

Query: 276 NCEIVLKHNGG-SPCDMADKVMSFLEQKGFLKA 308
           NCEI L   GG SP +MA+KV+ +L+ KG+L+A
Sbjct: 251 NCEISLGREGGTSPIEMAEKVVGYLDNKGYLQA 276

BLAST of Carg08118 vs. TAIR 10
Match: AT3G03900.1 (adenosine-5'-phosphosulfate (APS) kinase 3 )

HSP 1 Score: 320.9 bits (821), Expect = 1.1e-87
Identity = 153/206 (74.27%), Postives = 177/206 (85.92%), Query Frame = 0

Query: 104 LSTVGNSTNIKWHECSIGNVERQSLLNQKGCVIWITGLSGSGKSSVACALTKSLYKMGKL 163
           +STVGNSTNI W E  IG  ERQ LLNQKGCV+WITGLSGSGKS++AC+L++ L   GKL
Sbjct: 1   MSTVGNSTNIFWQESPIGKTERQKLLNQKGCVVWITGLSGSGKSTLACSLSRELNNRGKL 60

Query: 164 AYILDGDNVRHGLNRDLGFKAEDRAENIRRVGEVAKLFADAGVICIASLISPYRRDRDAC 223
           +YILDGDN+RHGLN+DLGFKAEDR ENIRRVGEVAKLFADAG+ICIASLISPYR+DRDAC
Sbjct: 61  SYILDGDNLRHGLNKDLGFKAEDRVENIRRVGEVAKLFADAGLICIASLISPYRKDRDAC 120

Query: 224 RAILPDGYFIEVFMDVPLEICEARDTKGLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKH 283
           R ++ +  FIEVFM++ L++CEARD KGLYKLARAGKIKGFTGIDDPYE PLNCEI LK 
Sbjct: 121 REMIQNSSFIEVFMNMSLQLCEARDPKGLYKLARAGKIKGFTGIDDPYESPLNCEIELKE 180

Query: 284 NGG---SPCDMADKVMSFLEQKGFLK 307
             G   SP  MA++V+S+LE KGFL+
Sbjct: 181 KEGECPSPVAMAEEVISYLEDKGFLQ 206

BLAST of Carg08118 vs. TAIR 10
Match: AT4G39940.1 (APS-kinase 2 )

HSP 1 Score: 320.9 bits (821), Expect = 1.1e-87
Identity = 149/199 (74.87%), Postives = 171/199 (85.93%), Query Frame = 0

Query: 112 NIKWHECSIGNVERQSLLNQKGCVIWITGLSGSGKSSVACALTKSLYKMGKLAYILDGDN 171
           NI WHE SI   +RQ LL QKGCV+WITGLSGSGKS+VACAL+K+L++ GKL Y LDGDN
Sbjct: 94  NIVWHESSICRCDRQQLLQQKGCVVWITGLSGSGKSTVACALSKALFERGKLTYTLDGDN 153

Query: 172 VRHGLNRDLGFKAEDRAENIRRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGY 231
           VRHGLNRDL FKAE R ENIRR+GEVAKLFAD GVICIASLISPYRRDRDACR++LPDG 
Sbjct: 154 VRHGLNRDLTFKAEHRTENIRRIGEVAKLFADVGVICIASLISPYRRDRDACRSLLPDGD 213

Query: 232 FIEVFMDVPLEICEARDTKGLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHNGG----S 291
           F+EVFMDVPL +CE+RD KGLYKLARAGKIKGFTGIDDPYE P+NCE+VLKH G     S
Sbjct: 214 FVEVFMDVPLHVCESRDPKGLYKLARAGKIKGFTGIDDPYEAPVNCEVVLKHTGDDESCS 273

Query: 292 PCDMADKVMSFLEQKGFLK 307
           P  MA+ ++S+L+ KG+L+
Sbjct: 274 PRQMAENIISYLQNKGYLE 292

BLAST of Carg08118 vs. TAIR 10
Match: AT5G67520.1 (adenosine-5'-phosphosulfate (APS) kinase 4 )

HSP 1 Score: 304.7 bits (779), Expect = 8.5e-83
Identity = 144/217 (66.36%), Postives = 172/217 (79.26%), Query Frame = 0

Query: 108 GNSTNIKWHECSIGNVERQSLLNQKGCVIWITGLSGSGKSSVACALTKSLYKMGKLAYIL 167
           G   NI WH+C +   +RQ L+ QKGCVIWITGLSGSGKSS+ACAL+++L+  GKL+YIL
Sbjct: 84  GKQKNIVWHDCPVTKSDRQELIKQKGCVIWITGLSGSGKSSLACALSRALHNRGKLSYIL 143

Query: 168 DGDNVRHGLNRDLGFKAEDRAENIRRVGEVAKLFADAGVICIASLISPYRRDRDACRAIL 227
           DGDNVRHGLN DL F+A+DRAENIRRVGEVAKLFAD+G+ICIASLISPYR +R ACRA+L
Sbjct: 144 DGDNVRHGLNSDLSFEADDRAENIRRVGEVAKLFADSGIICIASLISPYRIERAACRALL 203

Query: 228 PDGYFIEVFMDVPLEICEARDTKGLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKH---- 287
           P G FIEVFMDVPL +CEARD KGLYK ARAGKIKGFTG+DDPYE PL+CEIV+++    
Sbjct: 204 PQGDFIEVFMDVPLHVCEARDPKGLYKRARAGKIKGFTGVDDPYEAPLDCEIVIQNSRDK 263

Query: 288 --------------NGGSPCDMADKVMSFLEQKGFLK 307
                         +  S C+MAD V+S+L+Q G+LK
Sbjct: 264 GLSSSSSSSSSPSSSSSSLCEMADIVVSYLDQNGYLK 300

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7030942.14.7e-176100.00Adenylyl-sulfate kinase 1, chloroplastic, partial [Cucurbita argyrosperma subsp.... [more]
XP_022984565.14.7e-16896.09adenylyl-sulfate kinase 3-like [Cucurbita maxima][more]
XP_022943061.18.9e-16795.77adenylyl-sulfate kinase 3-like [Cucurbita moschata][more]
XP_023515167.11.9e-16495.13adenylyl-sulfate kinase 3-like [Cucurbita pepo subsp. pepo][more]
KAG6600284.11.4e-15194.39Endo-1,4-beta-xylanase 5-like protein, partial [Cucurbita argyrosperma subsp. so... [more]
Match NameE-valueIdentityDescription
Q432952.3e-9664.84Adenylyl-sulfate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APK1... [more]
O492044.1e-9066.27Adenylyl-sulfate kinase, chloroplastic OS=Catharanthus roseus OX=4058 GN=AKN PE=... [more]
O491961.6e-8674.87Adenylyl-sulfate kinase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APK2... [more]
Q9SRW71.6e-8674.27Adenylyl-sulfate kinase 3 OS=Arabidopsis thaliana OX=3702 GN=APK3 PE=1 SV=1[more]
Q84JF01.2e-8166.36Adenylyl-sulfate kinase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APK4... [more]
Match NameE-valueIdentityDescription
A0A6J1J8X72.3e-16896.09Adenylyl-sulfate kinase OS=Cucurbita maxima OX=3661 GN=LOC111482821 PE=3 SV=1[more]
A0A6J1FQP44.3e-16795.77Adenylyl-sulfate kinase OS=Cucurbita moschata OX=3662 GN=LOC111447912 PE=3 SV=1[more]
A0A1S3BYH91.1e-13881.43Adenylyl-sulfate kinase OS=Cucumis melo OX=3656 GN=LOC103494812 PE=3 SV=1[more]
A0A5A7TN801.1e-13881.43Adenylyl-sulfate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
A0A5D3DZZ63.2e-13881.37Adenylyl-sulfate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
Match NameE-valueIdentityDescription
AT2G14750.11.6e-9764.84APS kinase [more]
AT3G03900.11.1e-8774.27adenosine-5'-phosphosulfate (APS) kinase 3 [more]
AT4G39940.11.1e-8774.87APS-kinase 2 [more]
AT5G67520.18.5e-8366.36adenosine-5'-phosphosulfate (APS) kinase 4 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF01583APS_kinasecoord: 132..284
e-value: 1.9E-71
score: 239.1
NoneNo IPR availablePANTHERPTHR11055:SF55ADENYLYL-SULFATE KINASE 1, CHLOROPLASTICcoord: 89..307
NoneNo IPR availablePANTHERPTHR11055BIFUNCTIONAL 3'-PHOSPHOADENOSINE 5'-PHOSPHOSULFATE SYNTHASEcoord: 89..307
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 105..307
e-value: 2.8E-91
score: 306.8
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 131..302
IPR002891Adenylyl-sulfate kinaseTIGRFAMTIGR00455TIGR00455coord: 115..297
e-value: 8.6E-75
score: 248.4
IPR002891Adenylyl-sulfate kinaseHAMAPMF_00065Adenylyl_sulf_kinasecoord: 131..303
score: 36.076126
IPR002891Adenylyl-sulfate kinaseCDDcd02027APSKcoord: 135..282
e-value: 6.20127E-99
score: 285.526

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg08118-RACarg08118-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0070814 hydrogen sulfide biosynthetic process
biological_process GO:0016310 phosphorylation
biological_process GO:0000103 sulfate assimilation
molecular_function GO:0004020 adenylylsulfate kinase activity
molecular_function GO:0005524 ATP binding