Carg08103 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg08103
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Pyruvate kinase
Location	Carg_Chr04: 2289575 .. 2294445 (+)
RNA-Seq Expression	Carg08103
Synteny	Carg08103

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TGCAGGTTTTCGTCAATGGCGGAGGTGAGTGCTATGATGATTCGTGCTTGTTGCAATTTCGTTGCTAAAACCTCTGTGTTAGATGTGAAACGATCGGATTCTCGTTTGTCGGTTATCGGTCGAACAGTTTGGTGCGATCGGGGTAGGATGAAGGGGAGCGTTGTAACTGGAGTTAAGGCGGCAATGCAGGTGGATTTGGAGGATGATGAGAGGTTGAAACGATTGGACGGTTTAGAGGCGACATTAGGGCTTGATGTTGTGGCGGAGAGGGAATTGAAGGAGAAAGGGTTTTTGGGGATGAGGAAGACGAAGTTTGTTTGTACGATTGGGCCTGCGTGTTGTGCGATTGAGGATTTGGAGAAGTTGGCTTTGGAAGGTATGAACATTGCGAGATTGAATATGTGTCACAACAAGAAGGAGTGGCACTATGACGTGATTAGGAAGATCAAGAAGTTGAATGAAAAAGGATTTTGTATTTCGGTGATGATTGATACTGAAGGAAGTCAGATTCATGTTGTTGATCATGGAGCTCCTTCCTCCGTCAAAGTAGAGGTAGATTTTATATCTCTGTTATTTTTTCTGTAATTTTAGTGGTAATCAAATCAACAGAGAGTTATGATCCAGTGTTACTATTTTCCATTGTAATTTAAGAAAAACGGGGTTTAAATTCTCTGGCCATCATTGTTCAGGATGGTTCAAATTGGTTGTTTACTGCTGAGAAATTCGAGGGCTCTCGTCCATTTACTGTTCAAGCGAGCTATGAAGGCTTTTCTGAAGGTGCTGCACTTTGAATCTTTTTTTTTGTTTTCTCTGTCTTCTGAATTGTGATTAGAAGAAATGTATGAATCCCACAAGTTAAATAGGATATGAGTTTGAATTCTACAGGTATCCAAGTGGGTGATTCAATTGTGATTGATGGGGGAATGGCATCCTTTGAAGTTATGGAAAAGATTGGGAATGATTTAAGCTGTAAGTGCACAGACCCTGGTTTGTTTCTTCCTCGAGCAAAATGCAGCTTTTGGAGGGATGGGAGGCTTGTTATGAGAAAACATGAGTCTCCCACGCTGTCAAGTAAGGTAGGAAAGTGTCTGATGCATATCAATATTCATTCTGAATCATTTACTTGTGATGAAGCAGGTATTGTACGTTTGGAATTTCCCTTTCGAGCTCTCCTCAAAATTTTTAAAACGTGTCAGTTAGGAAGAGGGTTCCACACCCTTATAAAGAATGCTTCTCCTCTCCGACATGGAATCTCACAATCCACCCTCTTTGGGGCCAGCGTCTTCGCTGGCACTCGTTCCCCTCTCCAATGGACGTGGGATCTTTGAACCTCACAAGTTTTTACCCCTCCCCAACGGTGTGGACATGATGTATAAGGTTCACCGTTGAAAATTTTTCTTACTCTTGGGTTGTCAACAGGACAAGGTTTTACTGAATATGGTATGGCGACAAAAATGTTATGTTCATCATTAAAAATTTGTCTTCTCATAGTGGTTGTCAACATTATAAGATTTTGTTTCTGATTGGTATTGTGGATGACATTTTAGGATCATTGTTTAAATTTATTTTCTTGGGTTGTCAGCATCACAAGTTTTATCCCTGTATATCAACATCTATGTGCAGAAGAAATTTTATCCACTTTTCTGCAATTTTGCTCTCTTTTGACAAGGTTGACGATCGCATTAATGTGTCAAAATGTAAAAAATAATAACAAAAAAAAAAAAAGAGAGAGAGAAGAATTTAATGAATTACGTCTTCTTCTTGGATGATGCTAGTTCTCTGCTTTTCAACTCTGTTTTTCACAGTGTATTATACTATTTGGTTTACTATTTTATAGGATTGGTCCGACATTGAATTTGGAATCTCGGAAGGCGTCGACTTCATTGCCCTTTCCTTTGTGAACAGTGCAGATTCTGTCAAGCATCTAAAGAGCTACCTATCAGCAAAATCAGCAAAGTAAGCATCGATTCAAGAAGTCAACATATTAGCATGAGTTAGGTTGGAATATCACTACTAGGCATGAACGATATATCCCGATGTCCCTTTCAATTGAATTTTCATCACCACTGGTTTCTTAGCCGCAATTCATAAATTGTCTGGAAATCTTCTTTATTGAAACTGATATTGTGTAATTTCAAAACCCATTTTAGTAGTTAAGGATAAAAAAAATGTTCTCATGCACAGATCTGTGAGAGTATTGGCCAAGATTGAAAGCTTGGAGTCCCTTCAAAATCTAGAAGAAATCATAAAGGCTTCTGATGGAATCATGGTGGCTCGAGGGGATCTTGGGGTTGAAATTCCTCTTGAGCAGATACCAGCAGTCCAAGAAGAGATAACTAGTGTCTGTAGGGAGCTGAACAAACCAGTGATCGTAGCTTCTCAACTTCTAGAGTCGATGGTTGAATATCCTACTCCAACACGAGCTGAGGTACGAGTTTTTTAGTTAGTTAGTCTTTTTCAACTTCAGGTACAGGGATCCAACTATAATTCTCGTGAGATCCCACATCGGTTGGAGATGAGAACGAAACATTTCTTATAAAGGTGTGGAAACTTCTTCCTAGCAGATGCGTTTTAAAACCGTTAGACTGATAGTGATACGTAATGGGCCAAAGTAGACAATATCTACTAGTGGTGGGCTTAAGATGTTATAAATGGTATAGAGCCAAAATCGGGCACTGTGCCAGTGAGGACGTTGGGCCCTCAAGGGGAGTGGATTGTGAGACCTCACATCGGTTGGAAAAGGGAACGAAACATTCTTTATAAAGAGGGTAGAAACCTTTCCCTAACAGACATGTTTTAAAATTGTGAGGTTGAGACGATAGGGCCAAAACGGACAGTTATTCAAAAACATATTTCCGAGTTGAAATTTCTGGTGCTCTCTGGTATGGTGGATTGAATATTGTGTTGAAGTCTAGAATGATGATCAGTCTTCGTGTTCAATAGGTTGCAGATGTTTCTGAAGTTGTCCGACAGTATGCCGATGCATTGATGCTGTCTGGAGAGTCTGCCATAGGAGCATATGGACAGAAAGCGCTTTCGGTTCTGCAGATAGCCAGCAGCCGAATGGAGTTATGGAGTCGTGAAGAAAATACGAAACATTTCCTTCCCCAACACCAACTTGGGGTGTCATTGCACGATCGCATTGCCGAAGAAATTTGCAATTCTGCAGCTGAATTGGGTAAAACTCACTTTTCTCCACTTGCTTTCTTAACAATCCCTTCGTGTGTGATCATGAAAATAACATCAGAATCCCCTTTCGGTCATATATCATGTCTAACTTCTAGCTTCTTTATTACGTAATCATGCATAAGAAAATTATAAAGTTATTCTTTGTTAGTTTCAATTTGGTACTTTTTGTTTAGAAGTACATATTAACAAAATCTTTCATATCATCTTGGCTGTTCGCTTCTCAAAAAATTAAGTTTCGTTTGATGGGAGAGATAAACCATGGTAGATTGACTTAGTAGTCAATGTAAACGATAAAAGACTCGGACGAAATGAGTTCAAGACATGATGGTTATTTACCTAAGATTTAATATGTGTGACAGCCCAAGTCCACCGTTAGCAACTATTGTATGCTTTTGTCCCTTCACGATTTTAAAACGCGTCTGCTAGGGAGAAGTTTCTACACCCTTATAAAGAAAGTTTTGTTCTCCTCTCCAACCAATGTGGGCTCAGCATTCGCACTGTCATACCATTCGGTGTTTGACTCTGATACCATTTATAACAATCTAAGCCCACCGCTAACCGACATTGTCAATTTTATGTATTGTCGTCAGCCTCACAATTCTAAAACGCATCTATCAGAGAGAGATTTCCACACTCTCATAAATAATGTTTTGTTCCCCTCTTCAGCTAGGATAGGATCTATGAATTATCTTGAAAACCATATCTAATAGGGTTAGACAATTCTCTATGCCAACAGGGTTCGCTAGTTTAGACACTCACAGATATAAAAGTACAAATGGCTAGTGGTACTGAATTGGAGTAAAAAGAAAGTGTCGTATACAAAAGTAGATAACATTTAGCAACAACAGAGACATATTGTATCAAAAAAGTTATGGGTTGTGTCGTGGTGGGTGTGGTAGAACTGATTATATCATTTATAGGTTTCAAGAAACAATTCAATTACCTTTGAAAGGAAAGGTTCTGAAACTAGTTAACACCACCATGTGCTTTGTTGCAGCCAACAAACTTTCAGTAGACGCTATATTCGTGCTCACAAAGCAAGGTCACATGGCTTCTCTTCTATCCCGAAACCGCCCCAGCCCGCCTATATTCGCATTAACCGACGACGACAGCACGAGAATGGCTTTGAATCTTCAATGGGGAGTGTTTCCTCTGCGCATTGACCTCTCAGACGACATAGAAGCCAACATCTCAAGAGGAATCGAGCTAGTGAAGTCAAAGGGACTGGTCAAACAGGGGGACTTCGTGTTGATGGTCTCAGAGATCTCTCCAGCTCGAGCTGCATCAATGGCGTCCCAATCAATCCAGCTGAAATCCATCGTGTAGCACAAACAGAAAACCAAGGCAGGTCAGAGGTTTTATATACTGGAAACTGCTTTAGGATGCTTTGTAGTATGCTTTTTAGTGGTTTGTGATTGCACGTACCATATGTGGTGGCTACTCATTTATCTATCTCATTACATGTGAAACGACGTCGTTTTGTTACTTGTGGCCATGTCTGATAGGTTGCTTGCTGTAGTATAGCTAATATTTGGTGATTGAACAACTCTATTGAAATTATTGGCCATTGTTGATATGTTTGTAGAAAACGACAAAAGATGTGCCAAACTTTAGATCTGATTTTTAAAGTAATATTATTTATTTTATTATTAAATTTTAAGTTGTAGGAATTAGAAGTTGTA

mRNA sequence

TGCAGGTTTTCGTCAATGGCGGAGGTGAGTGCTATGATGATTCGTGCTTGTTGCAATTTCGTTGCTAAAACCTCTGTGTTAGATGTGAAACGATCGGATTCTCGTTTGTCGGTTATCGGTCGAACAGTTTGGTGCGATCGGGGTAGGATGAAGGGGAGCGTTGTAACTGGAGTTAAGGCGGCAATGCAGGTGGATTTGGAGGATGATGAGAGGTTGAAACGATTGGACGGTTTAGAGGCGACATTAGGGCTTGATGTTGTGGCGGAGAGGGAATTGAAGGAGAAAGGGTTTTTGGGGATGAGGAAGACGAAGTTTGTTTGTACGATTGGGCCTGCGTGTTGTGCGATTGAGGATTTGGAGAAGTTGGCTTTGGAAGGTATGAACATTGCGAGATTGAATATGTGTCACAACAAGAAGGAGTGGCACTATGACGTGATTAGGAAGATCAAGAAGTTGAATGAAAAAGGATTTTGTATTTCGGTGATGATTGATACTGAAGGAAGTCAGATTCATGTTGTTGATCATGGAGCTCCTTCCTCCGTCAAAGTAGAGGATGGTTCAAATTGGTTGTTTACTGCTGAGAAATTCGAGGGCTCTCGTCCATTTACTGTTCAAGCGAGCTATGAAGGCTTTTCTGAAGGTATCCAAGTGGGTGATTCAATTGTGATTGATGGGGGAATGGCATCCTTTGAAGTTATGGAAAAGATTGGGAATGATTTAAGCTGTAAGTGCACAGACCCTGGTTTGTTTCTTCCTCGAGCAAAATGCAGCTTTTGGAGGGATGGGAGGCTTGTTATGAGAAAACATGAGTCTCCCACGCTGTCAAGTAAGGATTGGTCCGACATTGAATTTGGAATCTCGGAAGGCGTCGACTTCATTGCCCTTTCCTTTGTGAACAGTGCAGATTCTGTCAAGCATCTAAAGAGCTACCTATCAGCAAAATCAGCAAAATCTGTGAGAGTATTGGCCAAGATTGAAAGCTTGGAGTCCCTTCAAAATCTAGAAGAAATCATAAAGGCTTCTGATGGAATCATGGTGGCTCGAGGGGATCTTGGGGTTGAAATTCCTCTTGAGCAGATACCAGCAGTCCAAGAAGAGATAACTAGTGTCTGTAGGGAGCTGAACAAACCAGTGATCGTAGCTTCTCAACTTCTAGAGTCGATGGTTGAATATCCTACTCCAACACGAGCTGAGGTTGCAGATGTTTCTGAAGTTGTCCGACAGTATGCCGATGCATTGATGCTGTCTGGAGAGTCTGCCATAGGAGCATATGGACAGAAAGCGCTTTCGGTTCTGCAGATAGCCAGCAGCCGAATGGAGTTATGGAGTCGTGAAGAAAATACGAAACATTTCCTTCCCCAACACCAACTTGGGGTGTCATTGCACGATCGCATTGCCGAAGAAATTTGCAATTCTGCAGCTGAATTGGCCAACAAACTTTCAGTAGACGCTATATTCGTGCTCACAAAGCAAGGTCACATGGCTTCTCTTCTATCCCGAAACCGCCCCAGCCCGCCTATATTCGCATTAACCGACGACGACAGCACGAGAATGGCTTTGAATCTTCAATGGGGAGTGTTTCCTCTGCGCATTGACCTCTCAGACGACATAGAAGCCAACATCTCAAGAGGAATCGAGCTAGTGAAGTCAAAGGGACTGGTCAAACAGGGGGACTTCGTGTTGATGGTCTCAGAGATCTCTCCAGCTCGAGCTGCATCAATGGCGTCCCAATCAATCCAGCTGAAATCCATCGTGTAGCACAAACAGAAAACCAAGGCAGTATGCTTTTTAGTGGTTTGTGATTGCACGTACCATATGTGGTGGCTACTCATTTATCTATCTCATTACATGTGAAACGACGTCGTTTTGTTACTTGTGGCCATGTCTGATAGGTTGCTTGCTGTAGTATAGCTAATATTTGGTGATTGAACAACTCTATTGAAATTATTGGCCATTGTTGATATGTTTGTAGAAAACGACAAAAGATGTGCCAAACTTTAGATCTGATTTTTAAAGTAATATTATTTATTTTATTATTAAATTTTAAGTTGTAGGAATTAGAAGTTGTA

Coding sequence (CDS)

ATGGCGGAGGTGAGTGCTATGATGATTCGTGCTTGTTGCAATTTCGTTGCTAAAACCTCTGTGTTAGATGTGAAACGATCGGATTCTCGTTTGTCGGTTATCGGTCGAACAGTTTGGTGCGATCGGGGTAGGATGAAGGGGAGCGTTGTAACTGGAGTTAAGGCGGCAATGCAGGTGGATTTGGAGGATGATGAGAGGTTGAAACGATTGGACGGTTTAGAGGCGACATTAGGGCTTGATGTTGTGGCGGAGAGGGAATTGAAGGAGAAAGGGTTTTTGGGGATGAGGAAGACGAAGTTTGTTTGTACGATTGGGCCTGCGTGTTGTGCGATTGAGGATTTGGAGAAGTTGGCTTTGGAAGGTATGAACATTGCGAGATTGAATATGTGTCACAACAAGAAGGAGTGGCACTATGACGTGATTAGGAAGATCAAGAAGTTGAATGAAAAAGGATTTTGTATTTCGGTGATGATTGATACTGAAGGAAGTCAGATTCATGTTGTTGATCATGGAGCTCCTTCCTCCGTCAAAGTAGAGGATGGTTCAAATTGGTTGTTTACTGCTGAGAAATTCGAGGGCTCTCGTCCATTTACTGTTCAAGCGAGCTATGAAGGCTTTTCTGAAGGTATCCAAGTGGGTGATTCAATTGTGATTGATGGGGGAATGGCATCCTTTGAAGTTATGGAAAAGATTGGGAATGATTTAAGCTGTAAGTGCACAGACCCTGGTTTGTTTCTTCCTCGAGCAAAATGCAGCTTTTGGAGGGATGGGAGGCTTGTTATGAGAAAACATGAGTCTCCCACGCTGTCAAGTAAGGATTGGTCCGACATTGAATTTGGAATCTCGGAAGGCGTCGACTTCATTGCCCTTTCCTTTGTGAACAGTGCAGATTCTGTCAAGCATCTAAAGAGCTACCTATCAGCAAAATCAGCAAAATCTGTGAGAGTATTGGCCAAGATTGAAAGCTTGGAGTCCCTTCAAAATCTAGAAGAAATCATAAAGGCTTCTGATGGAATCATGGTGGCTCGAGGGGATCTTGGGGTTGAAATTCCTCTTGAGCAGATACCAGCAGTCCAAGAAGAGATAACTAGTGTCTGTAGGGAGCTGAACAAACCAGTGATCGTAGCTTCTCAACTTCTAGAGTCGATGGTTGAATATCCTACTCCAACACGAGCTGAGGTTGCAGATGTTTCTGAAGTTGTCCGACAGTATGCCGATGCATTGATGCTGTCTGGAGAGTCTGCCATAGGAGCATATGGACAGAAAGCGCTTTCGGTTCTGCAGATAGCCAGCAGCCGAATGGAGTTATGGAGTCGTGAAGAAAATACGAAACATTTCCTTCCCCAACACCAACTTGGGGTGTCATTGCACGATCGCATTGCCGAAGAAATTTGCAATTCTGCAGCTGAATTGGCCAACAAACTTTCAGTAGACGCTATATTCGTGCTCACAAAGCAAGGTCACATGGCTTCTCTTCTATCCCGAAACCGCCCCAGCCCGCCTATATTCGCATTAACCGACGACGACAGCACGAGAATGGCTTTGAATCTTCAATGGGGAGTGTTTCCTCTGCGCATTGACCTCTCAGACGACATAGAAGCCAACATCTCAAGAGGAATCGAGCTAGTGAAGTCAAAGGGACTGGTCAAACAGGGGGACTTCGTGTTGATGGTCTCAGAGATCTCTCCAGCTCGAGCTGCATCAATGGCGTCCCAATCAATCCAGCTGAAATCCATCGTGTAG

Protein sequence

MAEVSAMMIRACCNFVAKTSVLDVKRSDSRLSVIGRTVWCDRGRMKGSVVTGVKAAMQVDLEDDERLKRLDGLEATLGLDVVAERELKEKGFLGMRKTKFVCTIGPACCAIEDLEKLALEGMNIARLNMCHNKKEWHYDVIRKIKKLNEKGFCISVMIDTEGSQIHVVDHGAPSSVKVEDGSNWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMASFEVMEKIGNDLSCKCTDPGLFLPRAKCSFWRDGRLVMRKHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADSVKHLKSYLSAKSAKSVRVLAKIESLESLQNLEEIIKASDGIMVARGDLGVEIPLEQIPAVQEEITSVCRELNKPVIVASQLLESMVEYPTPTRAEVADVSEVVRQYADALMLSGESAIGAYGQKALSVLQIASSRMELWSREENTKHFLPQHQLGVSLHDRIAEEICNSAAELANKLSVDAIFVLTKQGHMASLLSRNRPSPPIFALTDDDSTRMALNLQWGVFPLRIDLSDDIEANISRGIELVKSKGLVKQGDFVLMVSEISPARAASMASQSIQLKSIV

Homology

[Close]

BLAST of Carg08103 vs. NCBI nr
Match: KAG6600268.1 (Plastidial pyruvate kinase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7030927.1 Plastidial pyruvate kinase 1, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1128.6 bits (2918), Expect = 0.0e+00
Identity = 580/580 (100.00%), Postives = 580/580 (100.00%), Query Frame = 0


Query: 1   MAEVSAMMIRACCNFVAKTSVLDVKRSDSRLSVIGRTVWCDRGRMKGSVVTGVKAAMQVD 60
           MAEVSAMMIRACCNFVAKTSVLDVKRSDSRLSVIGRTVWCDRGRMKGSVVTGVKAAMQVD
Sbjct: 1   MAEVSAMMIRACCNFVAKTSVLDVKRSDSRLSVIGRTVWCDRGRMKGSVVTGVKAAMQVD 60

Query: 61  LEDDERLKRLDGLEATLGLDVVAERELKEKGFLGMRKTKFVCTIGPACCAIEDLEKLALE 120
           LEDDERLKRLDGLEATLGLDVVAERELKEKGFLGMRKTKFVCTIGPACCAIEDLEKLALE
Sbjct: 61  LEDDERLKRLDGLEATLGLDVVAERELKEKGFLGMRKTKFVCTIGPACCAIEDLEKLALE 120

Query: 121 GMNIARLNMCHNKKEWHYDVIRKIKKLNEKGFCISVMIDTEGSQIHVVDHGAPSSVKVED 180
           GMNIARLNMCHNKKEWHYDVIRKIKKLNEKGFCISVMIDTEGSQIHVVDHGAPSSVKVED
Sbjct: 121 GMNIARLNMCHNKKEWHYDVIRKIKKLNEKGFCISVMIDTEGSQIHVVDHGAPSSVKVED 180

Query: 181 GSNWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMASFEVMEKIGNDLSCKCT 240
           GSNWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMASFEVMEKIGNDLSCKCT
Sbjct: 181 GSNWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMASFEVMEKIGNDLSCKCT 240

Query: 241 DPGLFLPRAKCSFWRDGRLVMRKHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADSVK 300
           DPGLFLPRAKCSFWRDGRLVMRKHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADSVK
Sbjct: 241 DPGLFLPRAKCSFWRDGRLVMRKHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADSVK 300

Query: 301 HLKSYLSAKSAKSVRVLAKIESLESLQNLEEIIKASDGIMVARGDLGVEIPLEQIPAVQE 360
           HLKSYLSAKSAKSVRVLAKIESLESLQNLEEIIKASDGIMVARGDLGVEIPLEQIPAVQE
Sbjct: 301 HLKSYLSAKSAKSVRVLAKIESLESLQNLEEIIKASDGIMVARGDLGVEIPLEQIPAVQE 360

Query: 361 EITSVCRELNKPVIVASQLLESMVEYPTPTRAEVADVSEVVRQYADALMLSGESAIGAYG 420
           EITSVCRELNKPVIVASQLLESMVEYPTPTRAEVADVSEVVRQYADALMLSGESAIGAYG
Sbjct: 361 EITSVCRELNKPVIVASQLLESMVEYPTPTRAEVADVSEVVRQYADALMLSGESAIGAYG 420

Query: 421 QKALSVLQIASSRMELWSREENTKHFLPQHQLGVSLHDRIAEEICNSAAELANKLSVDAI 480
           QKALSVLQIASSRMELWSREENTKHFLPQHQLGVSLHDRIAEEICNSAAELANKLSVDAI
Sbjct: 421 QKALSVLQIASSRMELWSREENTKHFLPQHQLGVSLHDRIAEEICNSAAELANKLSVDAI 480

Query: 481 FVLTKQGHMASLLSRNRPSPPIFALTDDDSTRMALNLQWGVFPLRIDLSDDIEANISRGI 540
           FVLTKQGHMASLLSRNRPSPPIFALTDDDSTRMALNLQWGVFPLRIDLSDDIEANISRGI
Sbjct: 481 FVLTKQGHMASLLSRNRPSPPIFALTDDDSTRMALNLQWGVFPLRIDLSDDIEANISRGI 540

Query: 541 ELVKSKGLVKQGDFVLMVSEISPARAASMASQSIQLKSIV 581
           ELVKSKGLVKQGDFVLMVSEISPARAASMASQSIQLKSIV
Sbjct: 541 ELVKSKGLVKQGDFVLMVSEISPARAASMASQSIQLKSIV 580

[Close]

BLAST of Carg08103 vs. NCBI nr
Match: XP_022942487.1 (plastidial pyruvate kinase 1, chloroplastic-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1114.0 bits (2880), Expect = 0.0e+00
Identity = 573/580 (98.79%), Postives = 574/580 (98.97%), Query Frame = 0


Query: 1   MAEVSAMMIRACCNFVAKTSVLDVKRSDSRLSVIGRTVWCDRGRMKGSVVTGVKAAMQVD 60
           M EVSAMMIRACCNFVAKTSVLDVKRSDSRLSVIGRTVWCDRGRMKG VVTGVKAAMQVD
Sbjct: 1   MTEVSAMMIRACCNFVAKTSVLDVKRSDSRLSVIGRTVWCDRGRMKGRVVTGVKAAMQVD 60

Query: 61  LEDDERLKRLDGLEATLGLDVVAERELKEKGFLGMRKTKFVCTIGPACCAIEDLEKLALE 120
           LEDDERLKRLDGLEATLGLDVVAERELKEKGFLGMRKTKFVCTIGPACCAIEDLEKLALE
Sbjct: 61  LEDDERLKRLDGLEATLGLDVVAERELKEKGFLGMRKTKFVCTIGPACCAIEDLEKLALE 120

Query: 121 GMNIARLNMCHNKKEWHYDVIRKIKKLNEKGFCISVMIDTEGSQIHVVDHGAPSSVKVED 180
           GMNIARLNMCHNKKEWHYDVIRKIKKLNEKGFCISVMIDTEGSQIHVVDHGAP SVKVED
Sbjct: 121 GMNIARLNMCHNKKEWHYDVIRKIKKLNEKGFCISVMIDTEGSQIHVVDHGAPFSVKVED 180

Query: 181 GSNWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMASFEVMEKIGNDLSCKCT 240
           GSNWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMASFEVMEKIGNDLSCKCT
Sbjct: 181 GSNWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMASFEVMEKIGNDLSCKCT 240

Query: 241 DPGLFLPRAKCSFWRDGRLVMRKHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADSVK 300
           DPGLFLPRAKCSFWRDGRLVMRKHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADSVK
Sbjct: 241 DPGLFLPRAKCSFWRDGRLVMRKHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADSVK 300

Query: 301 HLKSYLSAKSAKSVRVLAKIESLESLQNLEEIIKASDGIMVARGDLGVEIPLEQIPAVQE 360
           HLKSYLSAKSAKSVRVLAKIESLESLQNLEEIIKASDGIMVARGDLGVEIPLEQIPAVQE
Sbjct: 301 HLKSYLSAKSAKSVRVLAKIESLESLQNLEEIIKASDGIMVARGDLGVEIPLEQIPAVQE 360

Query: 361 EITSVCRELNKPVIVASQLLESMVEYPTPTRAEVADVSEVVRQYADALMLSGESAIGAYG 420
           EITSVCRELNKPVIVASQLLESMVEYPTPTRAEVADVSEVVRQYADALML GESAIG+YG
Sbjct: 361 EITSVCRELNKPVIVASQLLESMVEYPTPTRAEVADVSEVVRQYADALMLCGESAIGSYG 420

Query: 421 QKALSVLQIASSRMELWSREENTKHFLPQHQLGVSLHDRIAEEICNSAAELANKLSVDAI 480
           QKALSVLQIASSRMELWS EENTKHFLPQHQLGVSLHDRIAEEICNSAAELANKLSVDAI
Sbjct: 421 QKALSVLQIASSRMELWSGEENTKHFLPQHQLGVSLHDRIAEEICNSAAELANKLSVDAI 480

Query: 481 FVLTKQGHMASLLSRNRPSPPIFALTDDDSTRMALNLQWGVFPLRIDLSDDIEANISRGI 540
           FVLTKQGHMASLLSRNRPSPPIFALTDDDSTRMALNLQWGVFPLRIDLSDDIEANISRGI
Sbjct: 481 FVLTKQGHMASLLSRNRPSPPIFALTDDDSTRMALNLQWGVFPLRIDLSDDIEANISRGI 540

Query: 541 ELVKSKGLVKQGDFVLMVSEISPARAASMASQSIQLKSIV 581
           ELVKSKGLVKQGDFVLMVSEIS ARAASMASQSIQLKSIV
Sbjct: 541 ELVKSKGLVKQGDFVLMVSEISAARAASMASQSIQLKSIV 580

[Close]

BLAST of Carg08103 vs. NCBI nr
Match: XP_022975726.1 (plastidial pyruvate kinase 1, chloroplastic-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1097.4 bits (2837), Expect = 0.0e+00
Identity = 565/580 (97.41%), Postives = 570/580 (98.28%), Query Frame = 0


Query: 1   MAEVSAMMIRACCNFVAKTSVLDVKRSDSRLSVIGRTVWCDRGRMKGSVVTGVKAAMQVD 60
           MAEVSAMMIRA CNFVAKTSVLDVKRSDSRLSVIGRTVWCDRGRMKG VVTGVKA MQVD
Sbjct: 1   MAEVSAMMIRARCNFVAKTSVLDVKRSDSRLSVIGRTVWCDRGRMKGRVVTGVKATMQVD 60

Query: 61  LEDDERLKRLDGLEATLGLDVVAERELKEKGFLGMRKTKFVCTIGPACCAIEDLEKLALE 120
           LEDDERLKRLDGLEATLGLDVVAERELKEKGFLGMRKTKFVCTIGPACCA EDLEKLALE
Sbjct: 61  LEDDERLKRLDGLEATLGLDVVAERELKEKGFLGMRKTKFVCTIGPACCATEDLEKLALE 120

Query: 121 GMNIARLNMCHNKKEWHYDVIRKIKKLNEKGFCISVMIDTEGSQIHVVDHGAPSSVKVED 180
           GMNIARLNMCHN KEWHY VIRKIKKLNEKGFCISVMIDTEGSQIHVVDHGAPSSVKVED
Sbjct: 121 GMNIARLNMCHNTKEWHYGVIRKIKKLNEKGFCISVMIDTEGSQIHVVDHGAPSSVKVED 180

Query: 181 GSNWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMASFEVMEKIGNDLSCKCT 240
           GSNWLFTAEKFEGSRPFTVQASY GFSEGIQVGDSIVIDGGMASFEVMEKIGNDLSCKCT
Sbjct: 181 GSNWLFTAEKFEGSRPFTVQASYGGFSEGIQVGDSIVIDGGMASFEVMEKIGNDLSCKCT 240

Query: 241 DPGLFLPRAKCSFWRDGRLVMRKHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADSVK 300
           DPGLFLPRAKCSFWRDGRLV+RKHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADSVK
Sbjct: 241 DPGLFLPRAKCSFWRDGRLVVRKHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADSVK 300

Query: 301 HLKSYLSAKSAKSVRVLAKIESLESLQNLEEIIKASDGIMVARGDLGVEIPLEQIPAVQE 360
           HLKSYLSAKSAKSVRVLAKIESLESLQNLEEII+ASDGIMVARGDLGVEIPLEQIPAVQE
Sbjct: 301 HLKSYLSAKSAKSVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQE 360

Query: 361 EITSVCRELNKPVIVASQLLESMVEYPTPTRAEVADVSEVVRQYADALMLSGESAIGAYG 420
           EITSVCRELNKPVIVASQLLESMVEYPTPTRAEVADVSE VRQYADALML GESAIG+YG
Sbjct: 361 EITSVCRELNKPVIVASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLCGESAIGSYG 420

Query: 421 QKALSVLQIASSRMELWSREENTKHFLPQHQLGVSLHDRIAEEICNSAAELANKLSVDAI 480
           QKALSVLQIASSRMELWSREEN KHFLPQHQLGVSLHDRIAEEICNSAAELANKLSVDAI
Sbjct: 421 QKALSVLQIASSRMELWSREENRKHFLPQHQLGVSLHDRIAEEICNSAAELANKLSVDAI 480

Query: 481 FVLTKQGHMASLLSRNRPSPPIFALTDDDSTRMALNLQWGVFPLRIDLSDDIEANISRGI 540
           FVLTKQGHMASLLSRNRPSPPIFALT++DSTRMALNLQWGVFPLRIDLSDDIEANISRGI
Sbjct: 481 FVLTKQGHMASLLSRNRPSPPIFALTNEDSTRMALNLQWGVFPLRIDLSDDIEANISRGI 540

Query: 541 ELVKSKGLVKQGDFVLMVSEISPARAASMASQSIQLKSIV 581
           ELVKSKGLVKQGDFVLMVSEISPARAASMASQSIQLKSIV
Sbjct: 541 ELVKSKGLVKQGDFVLMVSEISPARAASMASQSIQLKSIV 580

[Close]

BLAST of Carg08103 vs. NCBI nr
Match: XP_023515955.1 (plastidial pyruvate kinase 1, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1095.1 bits (2831), Expect = 0.0e+00
Identity = 563/580 (97.07%), Postives = 570/580 (98.28%), Query Frame = 0


Query: 1   MAEVSAMMIRACCNFVAKTSVLDVKRSDSRLSVIGRTVWCDRGRMKGSVVTGVKAAMQVD 60
           MAEVSAMMIRA CNFVA   VLDVKRSDSRLSVIGRTVWCDRGRMKG VVTGVKAAMQ+D
Sbjct: 1   MAEVSAMMIRARCNFVAINPVLDVKRSDSRLSVIGRTVWCDRGRMKGRVVTGVKAAMQMD 60

Query: 61  LEDDERLKRLDGLEATLGLDVVAERELKEKGFLGMRKTKFVCTIGPACCAIEDLEKLALE 120
           LEDDERLKRLDGLEATLGLDVVAERELKEKGFLGMRKTKFVCTIGPACCAI+DLEKLALE
Sbjct: 61  LEDDERLKRLDGLEATLGLDVVAERELKEKGFLGMRKTKFVCTIGPACCAIDDLEKLALE 120

Query: 121 GMNIARLNMCHNKKEWHYDVIRKIKKLNEKGFCISVMIDTEGSQIHVVDHGAPSSVKVED 180
           GMNIARLNMCHN KEWHYDVIRKIKKLNEKGFCISVMIDTEGSQIHVVDHGAPSSVKVED
Sbjct: 121 GMNIARLNMCHNTKEWHYDVIRKIKKLNEKGFCISVMIDTEGSQIHVVDHGAPSSVKVED 180

Query: 181 GSNWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMASFEVMEKIGNDLSCKCT 240
           GSNWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMASFEVMEKIGNDLSCKCT
Sbjct: 181 GSNWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMASFEVMEKIGNDLSCKCT 240

Query: 241 DPGLFLPRAKCSFWRDGRLVMRKHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADSVK 300
           DPGLFLPRAKCSFWR+GRLV+RKHESPTLSSKDWSDIEFGISEGVDFIALSFV+SA+SVK
Sbjct: 241 DPGLFLPRAKCSFWRNGRLVVRKHESPTLSSKDWSDIEFGISEGVDFIALSFVSSAESVK 300

Query: 301 HLKSYLSAKSAKSVRVLAKIESLESLQNLEEIIKASDGIMVARGDLGVEIPLEQIPAVQE 360
           HLKSYLSAKSAKSVRVLAKIESLESLQNLEEIIKASDGIMVARGDLGVEIPLEQIPAVQE
Sbjct: 301 HLKSYLSAKSAKSVRVLAKIESLESLQNLEEIIKASDGIMVARGDLGVEIPLEQIPAVQE 360

Query: 361 EITSVCRELNKPVIVASQLLESMVEYPTPTRAEVADVSEVVRQYADALMLSGESAIGAYG 420
           EITSVCRELNKPVIVASQLLESMVEYPTPTRAEVADVSE VRQYADALML GESAIG+YG
Sbjct: 361 EITSVCRELNKPVIVASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLCGESAIGSYG 420

Query: 421 QKALSVLQIASSRMELWSREENTKHFLPQHQLGVSLHDRIAEEICNSAAELANKLSVDAI 480
           QKALSVLQIASSRMELWSREEN KHFLPQHQLGVSLHDRIAEEICNSAAELANKLSVDAI
Sbjct: 421 QKALSVLQIASSRMELWSREENRKHFLPQHQLGVSLHDRIAEEICNSAAELANKLSVDAI 480

Query: 481 FVLTKQGHMASLLSRNRPSPPIFALTDDDSTRMALNLQWGVFPLRIDLSDDIEANISRGI 540
           FVLTKQGHMASLLSRNRPSPPIFALTDDDSTRM LNLQWGVFPLRIDLSDDIEANISRGI
Sbjct: 481 FVLTKQGHMASLLSRNRPSPPIFALTDDDSTRMGLNLQWGVFPLRIDLSDDIEANISRGI 540

Query: 541 ELVKSKGLVKQGDFVLMVSEISPARAASMASQSIQLKSIV 581
           ELVKSKGLVKQGDFVLMVSEISPARAASMASQSIQLKSIV
Sbjct: 541 ELVKSKGLVKQGDFVLMVSEISPARAASMASQSIQLKSIV 580

[Close]

BLAST of Carg08103 vs. NCBI nr
Match: XP_022942488.1 (plastidial pyruvate kinase 1, chloroplastic-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 1030.0 bits (2662), Expect = 7.8e-297
Identity = 530/536 (98.88%), Postives = 531/536 (99.07%), Query Frame = 0


Query: 45  MKGSVVTGVKAAMQVDLEDDERLKRLDGLEATLGLDVVAERELKEKGFLGMRKTKFVCTI 104
           MKG VVTGVKAAMQVDLEDDERLKRLDGLEATLGLDVVAERELKEKGFLGMRKTKFVCTI
Sbjct: 1   MKGRVVTGVKAAMQVDLEDDERLKRLDGLEATLGLDVVAERELKEKGFLGMRKTKFVCTI 60

Query: 105 GPACCAIEDLEKLALEGMNIARLNMCHNKKEWHYDVIRKIKKLNEKGFCISVMIDTEGSQ 164
           GPACCAIEDLEKLALEGMNIARLNMCHNKKEWHYDVIRKIKKLNEKGFCISVMIDTEGSQ
Sbjct: 61  GPACCAIEDLEKLALEGMNIARLNMCHNKKEWHYDVIRKIKKLNEKGFCISVMIDTEGSQ 120

Query: 165 IHVVDHGAPSSVKVEDGSNWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMAS 224
           IHVVDHGAP SVKVEDGSNWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMAS
Sbjct: 121 IHVVDHGAPFSVKVEDGSNWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMAS 180

Query: 225 FEVMEKIGNDLSCKCTDPGLFLPRAKCSFWRDGRLVMRKHESPTLSSKDWSDIEFGISEG 284
           FEVMEKIGNDLSCKCTDPGLFLPRAKCSFWRDGRLVMRKHESPTLSSKDWSDIEFGISEG
Sbjct: 181 FEVMEKIGNDLSCKCTDPGLFLPRAKCSFWRDGRLVMRKHESPTLSSKDWSDIEFGISEG 240

Query: 285 VDFIALSFVNSADSVKHLKSYLSAKSAKSVRVLAKIESLESLQNLEEIIKASDGIMVARG 344
           VDFIALSFVNSADSVKHLKSYLSAKSAKSVRVLAKIESLESLQNLEEIIKASDGIMVARG
Sbjct: 241 VDFIALSFVNSADSVKHLKSYLSAKSAKSVRVLAKIESLESLQNLEEIIKASDGIMVARG 300

Query: 345 DLGVEIPLEQIPAVQEEITSVCRELNKPVIVASQLLESMVEYPTPTRAEVADVSEVVRQY 404
           DLGVEIPLEQIPAVQEEITSVCRELNKPVIVASQLLESMVEYPTPTRAEVADVSEVVRQY
Sbjct: 301 DLGVEIPLEQIPAVQEEITSVCRELNKPVIVASQLLESMVEYPTPTRAEVADVSEVVRQY 360

Query: 405 ADALMLSGESAIGAYGQKALSVLQIASSRMELWSREENTKHFLPQHQLGVSLHDRIAEEI 464
           ADALML GESAIG+YGQKALSVLQIASSRMELWS EENTKHFLPQHQLGVSLHDRIAEEI
Sbjct: 361 ADALMLCGESAIGSYGQKALSVLQIASSRMELWSGEENTKHFLPQHQLGVSLHDRIAEEI 420

Query: 465 CNSAAELANKLSVDAIFVLTKQGHMASLLSRNRPSPPIFALTDDDSTRMALNLQWGVFPL 524
           CNSAAELANKLSVDAIFVLTKQGHMASLLSRNRPSPPIFALTDDDSTRMALNLQWGVFPL
Sbjct: 421 CNSAAELANKLSVDAIFVLTKQGHMASLLSRNRPSPPIFALTDDDSTRMALNLQWGVFPL 480

Query: 525 RIDLSDDIEANISRGIELVKSKGLVKQGDFVLMVSEISPARAASMASQSIQLKSIV 581
           RIDLSDDIEANISRGIELVKSKGLVKQGDFVLMVSEIS ARAASMASQSIQLKSIV
Sbjct: 481 RIDLSDDIEANISRGIELVKSKGLVKQGDFVLMVSEISAARAASMASQSIQLKSIV 536

[Close]

BLAST of Carg08103 vs. ExPASy Swiss-Prot
Match: Q9LIK0 (Plastidial pyruvate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PKP1 PE=1 SV=1)

HSP 1 Score: 595.9 bits (1535), Expect = 4.9e-169
Identity = 294/487 (60.37%), Postives = 369/487 (75.77%), Query Frame = 0


Query: 79  LDVVAERELKEKGFLGMRKTKFVCTIGPACCAIEDLEKLALEGMNIARLNMCHNKKEWHY 138
           +D V E ELKE GF   R+TK +CTIGPA C  E LE LA+ GMN+ARLNMCH  ++WH 
Sbjct: 100 VDTVTEAELKENGFRSTRRTKLICTIGPATCGFEQLEALAVGGMNVARLNMCHGTRDWHR 159

Query: 139 DVIRKIKKLN-EKGFCISVMIDTEGSQIHVVDHGAPSSVKVEDGSNWLFTAEKFEGSRP- 198
            VIR +++LN EKGF +++M+DTEGS+IH+ D G  +S K EDG  W FT   F+ SRP 
Sbjct: 160 GVIRSVRRLNEEKGFAVAIMMDTEGSEIHMGDLGGEASAKAEDGEVWTFTVRAFDSSRPE 219

Query: 199 FTVQASYEGFSEGIQVGDSIVIDGGMASFEVMEKIGNDLSCKCTDPGLFLPRAKCSFWRD 258
            T+  SY+GF+E ++VGD +++DGGM  FEV+EKIG D+ C CTDPGL LPRA  +FWRD
Sbjct: 220 RTISVSYDGFAEDVRVGDELLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRD 279

Query: 259 GRLVMRKHES-PTLSSKDWSDIEFGISEGVDFIALSFVNSADSVKHLKSYLSAKS-AKSV 318
           G LV  ++   PT+SSKDW DI+FGI+EGVDFIA+SFV SA+ + HLKSYL+A+S    +
Sbjct: 280 GSLVRERNAMLPTISSKDWLDIDFGIAEGVDFIAVSFVKSAEVINHLKSYLAARSRGGEI 339

Query: 319 RVLAKIESLESLQNLEEIIKASDGIMVARGDLGVEIPLEQIPAVQEEITSVCRELNKPVI 378
            V+AKIES++SL NLEEII ASDG MVARGDLG +IPLEQ+PA Q+ I  VCR LNKPVI
Sbjct: 340 GVIAKIESIDSLTNLEEIILASDGAMVARGDLGAQIPLEQVPAAQQRIVQVCRALNKPVI 399

Query: 379 VASQLLESMVEYPTPTRAEVADVSEVVRQYADALMLSGESAIGAYGQKALSVLQIASSRM 438
           VASQLLESM+EYPTPTRAEVADVSE VRQ +DALMLSGESA+G +  KAL+VL+  S R+
Sbjct: 400 VASQLLESMIEYPTPTRAEVADVSEAVRQRSDALMLSGESAMGQFPDKALTVLRTVSLRI 459

Query: 439 ELWSREENTKHFLPQHQLGVSLHDRIAEEICNSAAELANKLSVDAIFVLTKQGHMASLLS 498
           E W REE     +P   +G S  D+I+EEICNSAA++AN L VDA+FV T  GHMASL+S
Sbjct: 460 ERWWREEKRHESVPLQAIGSSFSDKISEEICNSAAKMANNLGVDAVFVYTTSGHMASLVS 519

Query: 499 RNRPSPPIFALTDDDSTRMALNLQWGVFPLRIDLSDDIEANISRGIELVKSKGLVKQGDF 558
           R RP  PIFA T   S R  LNLQWG+ P R+  SDD+E+N+++   L+KS+G++K GD 
Sbjct: 520 RCRPDCPIFAFTTTTSVRRRLNLQWGLIPFRLSFSDDMESNLNKTFSLLKSRGMIKSGDL 579

Query: 559 VLMVSEI 562
           V+ VS++
Sbjct: 580 VIAVSDM 586

[Close]

BLAST of Carg08103 vs. ExPASy Swiss-Prot
Match: Q43117 (Pyruvate kinase isozyme A, chloroplastic OS=Ricinus communis OX=3988 PE=1 SV=1)

HSP 1 Score: 595.5 bits (1534), Expect = 6.4e-169
Identity = 290/491 (59.06%), Postives = 377/491 (76.78%), Query Frame = 0


Query: 75  ATLGLDVVAERELKEKGFLGMRKTKFVCTIGPACCAIEDLEKLALEGMNIARLNMCHNKK 134
           +++ +D V E ELKE GF   R+TK VCTIGPA C  E+LE LA+ GMN+AR+NMCH  +
Sbjct: 83  SSIEVDAVTETELKENGFRSTRRTKLVCTIGPATCGFEELEALAVGGMNVARINMCHGTR 142

Query: 135 EWHYDVIRKIKKLN-EKGFCISVMIDTEGSQIHVVDHGAPSSVKVEDGSNWLFTAEKFEG 194
           EWH  VI ++++LN EKGF +++M+DTEGS+IH+ D G  SS K EDG  W F+   ++ 
Sbjct: 143 EWHKSVIERVRRLNEEKGFAVAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAYDS 202

Query: 195 SRP-FTVQASYEGFSEGIQVGDSIVIDGGMASFEVMEKIGNDLSCKCTDPGLFLPRAKCS 254
            RP  T+  +Y+GF+E ++VGD +++DGGM  FEV+EKIG D+ C+CTDPGL LPRA  +
Sbjct: 203 PRPERTINVNYDGFAEDVKVGDELLVDGGMVRFEVIEKIGPDVKCRCTDPGLLLPRANLT 262

Query: 255 FWRDGRLVMRKHES-PTLSSKDWSDIEFGISEGVDFIALSFVNSADSVKHLKSYLSAKSA 314
           FWRDG LV  ++   PT+SSKDW DI+FGI+EGVDFIA+SFV SA+ + HLKSY++A+S 
Sbjct: 263 FWRDGSLVRERNAMLPTISSKDWLDIDFGIAEGVDFIAISFVKSAEVINHLKSYIAARSR 322

Query: 315 KS-VRVLAKIESLESLQNLEEIIKASDGIMVARGDLGVEIPLEQIPAVQEEITSVCRELN 374
            S + V+AKIES++SL+NLEEII+ASDG MVARGDLG +IPLEQ+P+ Q+ I  VCR+LN
Sbjct: 323 DSDIAVIAKIESIDSLKNLEEIIRASDGAMVARGDLGAQIPLEQVPSAQQNIVQVCRQLN 382

Query: 375 KPVIVASQLLESMVEYPTPTRAEVADVSEVVRQYADALMLSGESAIGAYGQKALSVLQIA 434
           KPVIVASQLLESM+EYPTPTRAEVADVSE VRQ ADALMLSGESA+G Y +KAL+VL+  
Sbjct: 383 KPVIVASQLLESMIEYPTPTRAEVADVSEAVRQRADALMLSGESAMGQYPEKALAVLRSV 442

Query: 435 SSRMELWSREENTKHFLPQHQLGVSLHDRIAEEICNSAAELANKLSVDAIFVLTKQGHMA 494
           S R+E W REE     +    +G +  D I+EEICNSAA++AN L VDA+FV TK GHMA
Sbjct: 443 SVRIEKWWREEKHHEAMELPAIGSTYSDSISEEICNSAAKMANNLGVDALFVYTKDGHMA 502

Query: 495 SLLSRNRPSPPIFALTDDDSTRMALNLQWGVFPLRIDLSDDIEANISRGIELVKSKGLVK 554
           SLLSR RP  PIFA T   S R  LNLQWG+ P R+  +DD+E+N+++   L+K++G++K
Sbjct: 503 SLLSRCRPDCPIFAFTTTTSVRRRLNLQWGLIPFRLSFADDMESNLNKTFSLLKARGMIK 562

Query: 555 QGDFVLMVSEI 562
            GD V+ VS++
Sbjct: 563 SGDLVIAVSDM 573

[Close]

BLAST of Carg08103 vs. ExPASy Swiss-Prot
Match: Q40545 (Pyruvate kinase isozyme A, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 578.6 bits (1490), Expect = 8.1e-164
Identity = 284/491 (57.84%), Postives = 372/491 (75.76%), Query Frame = 0


Query: 75  ATLGLDVVAERELKEKGFLGMRKTKFVCTIGPACCAIEDLEKLALEGMNIARLNMCHNKK 134
           +++ +D V E ELKE GF   R+TK +CTIGPA C  E LE+LA  GMN+AR+NMCH  +
Sbjct: 93  SSIEVDTVTEAELKENGFRSTRRTKLICTIGPATCGFEQLERLAEGGMNVARINMCHGTR 152

Query: 135 EWHYDVIRKIKKLN-EKGFCISVMIDTEGSQIHVVDHGAPSSVKVEDGSNWLFTAEKFEG 194
           EWH  VI ++++LN EKGF +++M+DTEGS+IH+ D G  SS K EDG  W FT   F+ 
Sbjct: 153 EWHRMVIERLRRLNEEKGFAVAIMMDTEGSEIHMGDLGGASSAKAEDGEIWNFTVRSFDP 212

Query: 195 SRP-FTVQASYEGFSEGIQVGDSIVIDGGMASFEVMEKIGNDLSCKCTDPGLFLPRAKCS 254
             P  TV  +Y+GF+E ++VGD +++DGGM  FEV+EKIG D+ C CTDPGL LPRA  +
Sbjct: 213 PLPERTVTVNYDGFAEDVKVGDELLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLT 272

Query: 255 FWRDGRLVMRKHES-PTLSSKDWSDIEFGISEGVDFIALSFVNSADSVKHLKSYLSAKSA 314
           FWRDG+LV  ++   PT+SSKDW DI+FGI+EGVDFIA+SFV SA+ +KHLKSY+ A++ 
Sbjct: 273 FWRDGKLVRERNAMLPTISSKDWLDIDFGIAEGVDFIAVSFVKSAEVIKHLKSYIQARAR 332

Query: 315 KS-VRVLAKIESLESLQNLEEIIKASDGIMVARGDLGVEIPLEQIPAVQEEITSVCRELN 374
            S + V+AKIES++SL+NLEEII+ASDG MVARGDLG +IPLEQ+P+ Q++I  +CR+LN
Sbjct: 333 DSDISVIAKIESIDSLKNLEEIIQASDGAMVARGDLGAQIPLEQVPSEQQKIVQICRQLN 392

Query: 375 KPVIVASQLLESMVEYPTPTRAEVADVSEVVRQYADALMLSGESAIGAYGQKALSVLQIA 434
           +PVIVASQLLESM+EYP PTRAEVADVSE VRQ  DALMLSGESA+G + +KAL+VL+  
Sbjct: 393 RPVIVASQLLESMIEYPIPTRAEVADVSEAVRQRGDALMLSGESAMGQFPEKALTVLRSV 452

Query: 435 SSRMELWSREENTKHFLPQHQLGVSLHDRIAEEICNSAAELANKLSVDAIFVLTKQGHMA 494
           S R+E   RE+     +    +  S  D I+EEICNSAA++AN L VDA+FV TK GHMA
Sbjct: 453 SLRIERMWREQKRHEVIELPSIASSFSDSISEEICNSAAKMANNLEVDALFVYTKNGHMA 512

Query: 495 SLLSRNRPSPPIFALTDDDSTRMALNLQWGVFPLRIDLSDDIEANISRGIELVKSKGLVK 554
           SLLSR RP  PIFA T   S R  LNLQWG+ P R+  SDD+E+N+++   L+K++G++K
Sbjct: 513 SLLSRCRPDCPIFAFTTTTSVRRRLNLQWGLMPFRLSFSDDMESNLNKTFSLLKARGMIK 572

Query: 555 QGDFVLMVSEI 562
            GD ++ VS++
Sbjct: 573 SGDLIIAVSDM 583

[Close]

BLAST of Carg08103 vs. ExPASy Swiss-Prot
Match: Q40546 (Pyruvate kinase isozyme G, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 322.8 bits (826), Expect = 8.0e-87
Identity = 191/486 (39.30%), Postives = 286/486 (58.85%), Query Frame = 0


Query: 96  RKTKFVCTIGPACCAIEDLEKLALEGMNIARLNMCHNKKEWHYDVIRKIKKLNE--KGFC 155
           RKTK VCTIGP+  + E + KLA  GMN+ARLNM H     H   I  +K+ N   +   
Sbjct: 91  RKTKIVCTIGPSTSSREMIWKLAEAGMNVARLNMSHGDHASHQRTIDLVKEYNAQFEDKV 150

Query: 156 ISVMIDTEGSQIHVVDHGAPSSVKVEDGSNWLFTAEKFEGSRPFTVQASYEGFSEGIQVG 215
           I++M+DT+G +  V+    P  + +++G  + F+ ++   +   TV  +Y+ F   ++ G
Sbjct: 151 IAIMLDTKGPE--VISGDVPKPILLKEGQEFNFSIKRGVSTED-TVSVNYDDFINDVEAG 210

Query: 216 DSIVIDGGMASFEVMEKIGNDLSCKCTDPGLFLPRAKCSFWRDGRLVMRKHESPTLSSKD 275
           D +++DGGM S  V  K  + + C+  D G    R      R   +  +    P+++ KD
Sbjct: 211 DILLVDGGMMSLAVKSKTSDIVKCEVIDGGELKSR------RHLNVRGKSATLPSITEKD 270

Query: 276 WSDIEFGISEGVDFIALSFVNSADSVKHLKSYLSAKSAKSVRVLAKIESLESLQNLEEII 335
           W DI+FG++  VDF A+SFV  A  V  LK YL + +A  + V+ KIES +S+ NL  II
Sbjct: 271 WDDIKFGVNNQVDFYAVSFVKDAKVVHELKDYLKSCNA-DIHVIVKIESADSIPNLHSII 330

Query: 336 KASDGIMVARGDLGVEIPLEQIPAVQEEITSVCRELNKPVIVASQLLESMVEYPTPTRAE 395
            ASDG MVARGDLG E+P+E++P +QE+I   C+ + KPVIVA+ +LESM+++PTPTRAE
Sbjct: 331 SASDGAMVARGDLGAELPIEEVPLLQEDIIRRCQSMQKPVIVATNMLESMIDHPTPTRAE 390

Query: 396 VADVSEVVRQYADALMLSGESAIGAYGQKALSVLQIASSRMELWSREENTKHFLPQHQLG 455
           V+D+S  VR+ ADA+MLSGE+A G Y  KA+ V+ I + R E  S +++T    P     
Sbjct: 391 VSDISIAVREGADAVMLSGETAHGKYPLKAVKVMHIVALRTES-SLQKSTSS--PSQSAA 450

Query: 456 VSLHDRIAEEICNSAAELANKLSVDAIFVLTKQGHMASLLSRNRPSPPIFALTDDDSTRM 515
              H  + E     ++ +AN LS   I V T+ G MA +LS NRPS  +FA T+++  + 
Sbjct: 451 YKSH--MGEMFAFHSSSMANTLST-PIIVFTRTGSMAIILSHNRPSSTVFAFTNNERVKQ 510

Query: 516 ALNLQWGVFPLRIDLSDDIEANISRGIELVKSKGLVKQGDFVLMVSEISPARAASMASQS 575
            L L  GV P+ ++ S D E   SR I+L+ SK LVK G +V +V   +       ++  
Sbjct: 511 RLALYHGVVPIYMEFSSDAEETFSRAIKLLLSKSLVKDGQYVTLVQSGAQPIWRRHSTHH 560

Query: 576 IQLKSI 580
           IQ++ +
Sbjct: 571 IQVRKV 560

[Close]

BLAST of Carg08103 vs. ExPASy Swiss-Prot
Match: Q9FLW9 (Plastidial pyruvate kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PKP2 PE=1 SV=1)

HSP 1 Score: 314.7 bits (805), Expect = 2.2e-84
Identity = 185/486 (38.07%), Postives = 280/486 (57.61%), Query Frame = 0


Query: 96  RKTKFVCTIGPACCAIEDLEKLALEGMNIARLNMCHNKKEWHYDVIRKIKKLN--EKGFC 155
           RKTK VCT+GP+    E + KLA  GMN+AR+NM H     H  VI  +K+ N   K   
Sbjct: 110 RKTKIVCTVGPSTNTREMIWKLAEAGMNVARMNMSHGDHASHKKVIDLVKEYNAQTKDNT 169

Query: 156 ISVMIDTEGSQIHVVDHGAPSSVKVEDGSNWLFTAEKFEGSRPFTVQASYEGFSEGIQVG 215
           I++M+DT+G ++   D   P  + ++ G  + FT E+   S P  V  +Y+ F   ++ G
Sbjct: 170 IAIMLDTKGPEVRSGD--LPQPIMLDPGQEFTFTIER-GVSTPSCVSVNYDDFVNDVEAG 229

Query: 216 DSIVIDGGMASFEVMEKIGNDLSCKCTDPGLFLPRAKCSFWRDGRLVMRKHESPTLSSKD 275
           D +++DGGM SF V  K  + + C+  D G    R      R   +  +    P+++ KD
Sbjct: 230 DMLLVDGGMMSFMVKSKTKDSVKCEVVDGGELKSR------RHLNVRGKSATLPSITEKD 289

Query: 276 WSDIEFGISEGVDFIALSFVNSADSVKHLKSYLSAKSAKSVRVLAKIESLESLQNLEEII 335
           W DI+FG+   VDF A+SFV  A  V  LK YL   S   + V+ KIES +S+ NL  II
Sbjct: 290 WEDIKFGVENKVDFYAVSFVKDAQVVHELKKYLQ-NSGADIHVIVKIESADSIPNLHSII 349

Query: 336 KASDGIMVARGDLGVEIPLEQIPAVQEEITSVCRELNKPVIVASQLLESMVEYPTPTRAE 395
            ASDG MVARGDLG E+P+E++P +QEEI ++CR + K VIVA+ +LESM+ +PTPTRAE
Sbjct: 350 TASDGAMVARGDLGAELPIEEVPILQEEIINLCRSMGKAVIVATNMLESMIVHPTPTRAE 409

Query: 396 VADVSEVVRQYADALMLSGESAIGAYGQKALSVLQIASSRMELWSREENTKHFLPQHQLG 455
           V+D++  VR+ ADA+MLSGE+A G +  KA  V+   + R E       T   +P + LG
Sbjct: 410 VSDIAIAVREGADAVMLSGETAHGKFPLKAAGVMHTVALRTE----ATITSGEMPPN-LG 469

Query: 456 VSLHDRIAEEICNSAAELANKLSVDAIFVLTKQGHMASLLSRNRPSPPIFALTDDDSTRM 515
            +  + ++E     A  ++N L    + V T+ G MA LLS  RPS  I+A T++   + 
Sbjct: 470 QAFKNHMSEMFAYHATMMSNTLGTSTV-VFTRTGFMAILLSHYRPSGTIYAFTNEKKIQQ 529

Query: 516 ALNLQWGVFPLRIDLSDDIEANISRGIELVKSKGLVKQGDFVLMVSEISPARAASMASQS 575
            L L  GV P+ ++ +DD E   +  +  +  +G+VK+G+ + +V   +     S ++ +
Sbjct: 530 RLALYQGVCPIYMEFTDDAEETFANALATLLKQGMVKKGEEIAIVQSGTQPIWRSQSTHN 579

Query: 576 IQLKSI 580
           IQ++ +
Sbjct: 590 IQVRKV 579

[Close]

BLAST of Carg08103 vs. ExPASy TrEMBL
Match: A0A6J1FQD7 (Pyruvate kinase OS=Cucurbita moschata OX=3662 GN=LOC111447508 PE=3 SV=1)

HSP 1 Score: 1114.0 bits (2880), Expect = 0.0e+00
Identity = 573/580 (98.79%), Postives = 574/580 (98.97%), Query Frame = 0


Query: 1   MAEVSAMMIRACCNFVAKTSVLDVKRSDSRLSVIGRTVWCDRGRMKGSVVTGVKAAMQVD 60
           M EVSAMMIRACCNFVAKTSVLDVKRSDSRLSVIGRTVWCDRGRMKG VVTGVKAAMQVD
Sbjct: 1   MTEVSAMMIRACCNFVAKTSVLDVKRSDSRLSVIGRTVWCDRGRMKGRVVTGVKAAMQVD 60

Query: 61  LEDDERLKRLDGLEATLGLDVVAERELKEKGFLGMRKTKFVCTIGPACCAIEDLEKLALE 120
           LEDDERLKRLDGLEATLGLDVVAERELKEKGFLGMRKTKFVCTIGPACCAIEDLEKLALE
Sbjct: 61  LEDDERLKRLDGLEATLGLDVVAERELKEKGFLGMRKTKFVCTIGPACCAIEDLEKLALE 120

Query: 121 GMNIARLNMCHNKKEWHYDVIRKIKKLNEKGFCISVMIDTEGSQIHVVDHGAPSSVKVED 180
           GMNIARLNMCHNKKEWHYDVIRKIKKLNEKGFCISVMIDTEGSQIHVVDHGAP SVKVED
Sbjct: 121 GMNIARLNMCHNKKEWHYDVIRKIKKLNEKGFCISVMIDTEGSQIHVVDHGAPFSVKVED 180

Query: 181 GSNWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMASFEVMEKIGNDLSCKCT 240
           GSNWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMASFEVMEKIGNDLSCKCT
Sbjct: 181 GSNWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMASFEVMEKIGNDLSCKCT 240

Query: 241 DPGLFLPRAKCSFWRDGRLVMRKHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADSVK 300
           DPGLFLPRAKCSFWRDGRLVMRKHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADSVK
Sbjct: 241 DPGLFLPRAKCSFWRDGRLVMRKHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADSVK 300

Query: 301 HLKSYLSAKSAKSVRVLAKIESLESLQNLEEIIKASDGIMVARGDLGVEIPLEQIPAVQE 360
           HLKSYLSAKSAKSVRVLAKIESLESLQNLEEIIKASDGIMVARGDLGVEIPLEQIPAVQE
Sbjct: 301 HLKSYLSAKSAKSVRVLAKIESLESLQNLEEIIKASDGIMVARGDLGVEIPLEQIPAVQE 360

Query: 361 EITSVCRELNKPVIVASQLLESMVEYPTPTRAEVADVSEVVRQYADALMLSGESAIGAYG 420
           EITSVCRELNKPVIVASQLLESMVEYPTPTRAEVADVSEVVRQYADALML GESAIG+YG
Sbjct: 361 EITSVCRELNKPVIVASQLLESMVEYPTPTRAEVADVSEVVRQYADALMLCGESAIGSYG 420

Query: 421 QKALSVLQIASSRMELWSREENTKHFLPQHQLGVSLHDRIAEEICNSAAELANKLSVDAI 480
           QKALSVLQIASSRMELWS EENTKHFLPQHQLGVSLHDRIAEEICNSAAELANKLSVDAI
Sbjct: 421 QKALSVLQIASSRMELWSGEENTKHFLPQHQLGVSLHDRIAEEICNSAAELANKLSVDAI 480

Query: 481 FVLTKQGHMASLLSRNRPSPPIFALTDDDSTRMALNLQWGVFPLRIDLSDDIEANISRGI 540
           FVLTKQGHMASLLSRNRPSPPIFALTDDDSTRMALNLQWGVFPLRIDLSDDIEANISRGI
Sbjct: 481 FVLTKQGHMASLLSRNRPSPPIFALTDDDSTRMALNLQWGVFPLRIDLSDDIEANISRGI 540

Query: 541 ELVKSKGLVKQGDFVLMVSEISPARAASMASQSIQLKSIV 581
           ELVKSKGLVKQGDFVLMVSEIS ARAASMASQSIQLKSIV
Sbjct: 541 ELVKSKGLVKQGDFVLMVSEISAARAASMASQSIQLKSIV 580

[Close]

BLAST of Carg08103 vs. ExPASy TrEMBL
Match: A0A6J1IF03 (Pyruvate kinase OS=Cucurbita maxima OX=3661 GN=LOC111475842 PE=3 SV=1)

HSP 1 Score: 1097.4 bits (2837), Expect = 0.0e+00
Identity = 565/580 (97.41%), Postives = 570/580 (98.28%), Query Frame = 0


Query: 1   MAEVSAMMIRACCNFVAKTSVLDVKRSDSRLSVIGRTVWCDRGRMKGSVVTGVKAAMQVD 60
           MAEVSAMMIRA CNFVAKTSVLDVKRSDSRLSVIGRTVWCDRGRMKG VVTGVKA MQVD
Sbjct: 1   MAEVSAMMIRARCNFVAKTSVLDVKRSDSRLSVIGRTVWCDRGRMKGRVVTGVKATMQVD 60

Query: 61  LEDDERLKRLDGLEATLGLDVVAERELKEKGFLGMRKTKFVCTIGPACCAIEDLEKLALE 120
           LEDDERLKRLDGLEATLGLDVVAERELKEKGFLGMRKTKFVCTIGPACCA EDLEKLALE
Sbjct: 61  LEDDERLKRLDGLEATLGLDVVAERELKEKGFLGMRKTKFVCTIGPACCATEDLEKLALE 120

Query: 121 GMNIARLNMCHNKKEWHYDVIRKIKKLNEKGFCISVMIDTEGSQIHVVDHGAPSSVKVED 180
           GMNIARLNMCHN KEWHY VIRKIKKLNEKGFCISVMIDTEGSQIHVVDHGAPSSVKVED
Sbjct: 121 GMNIARLNMCHNTKEWHYGVIRKIKKLNEKGFCISVMIDTEGSQIHVVDHGAPSSVKVED 180

Query: 181 GSNWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMASFEVMEKIGNDLSCKCT 240
           GSNWLFTAEKFEGSRPFTVQASY GFSEGIQVGDSIVIDGGMASFEVMEKIGNDLSCKCT
Sbjct: 181 GSNWLFTAEKFEGSRPFTVQASYGGFSEGIQVGDSIVIDGGMASFEVMEKIGNDLSCKCT 240

Query: 241 DPGLFLPRAKCSFWRDGRLVMRKHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADSVK 300
           DPGLFLPRAKCSFWRDGRLV+RKHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADSVK
Sbjct: 241 DPGLFLPRAKCSFWRDGRLVVRKHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADSVK 300

Query: 301 HLKSYLSAKSAKSVRVLAKIESLESLQNLEEIIKASDGIMVARGDLGVEIPLEQIPAVQE 360
           HLKSYLSAKSAKSVRVLAKIESLESLQNLEEII+ASDGIMVARGDLGVEIPLEQIPAVQE
Sbjct: 301 HLKSYLSAKSAKSVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQE 360

Query: 361 EITSVCRELNKPVIVASQLLESMVEYPTPTRAEVADVSEVVRQYADALMLSGESAIGAYG 420
           EITSVCRELNKPVIVASQLLESMVEYPTPTRAEVADVSE VRQYADALML GESAIG+YG
Sbjct: 361 EITSVCRELNKPVIVASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLCGESAIGSYG 420

Query: 421 QKALSVLQIASSRMELWSREENTKHFLPQHQLGVSLHDRIAEEICNSAAELANKLSVDAI 480
           QKALSVLQIASSRMELWSREEN KHFLPQHQLGVSLHDRIAEEICNSAAELANKLSVDAI
Sbjct: 421 QKALSVLQIASSRMELWSREENRKHFLPQHQLGVSLHDRIAEEICNSAAELANKLSVDAI 480

Query: 481 FVLTKQGHMASLLSRNRPSPPIFALTDDDSTRMALNLQWGVFPLRIDLSDDIEANISRGI 540
           FVLTKQGHMASLLSRNRPSPPIFALT++DSTRMALNLQWGVFPLRIDLSDDIEANISRGI
Sbjct: 481 FVLTKQGHMASLLSRNRPSPPIFALTNEDSTRMALNLQWGVFPLRIDLSDDIEANISRGI 540

Query: 541 ELVKSKGLVKQGDFVLMVSEISPARAASMASQSIQLKSIV 581
           ELVKSKGLVKQGDFVLMVSEISPARAASMASQSIQLKSIV
Sbjct: 541 ELVKSKGLVKQGDFVLMVSEISPARAASMASQSIQLKSIV 580

[Close]

BLAST of Carg08103 vs. ExPASy TrEMBL
Match: A0A6J1FUY6 (Pyruvate kinase OS=Cucurbita moschata OX=3662 GN=LOC111447508 PE=3 SV=1)

HSP 1 Score: 1030.0 bits (2662), Expect = 3.8e-297
Identity = 530/536 (98.88%), Postives = 531/536 (99.07%), Query Frame = 0


Query: 45  MKGSVVTGVKAAMQVDLEDDERLKRLDGLEATLGLDVVAERELKEKGFLGMRKTKFVCTI 104
           MKG VVTGVKAAMQVDLEDDERLKRLDGLEATLGLDVVAERELKEKGFLGMRKTKFVCTI
Sbjct: 1   MKGRVVTGVKAAMQVDLEDDERLKRLDGLEATLGLDVVAERELKEKGFLGMRKTKFVCTI 60

Query: 105 GPACCAIEDLEKLALEGMNIARLNMCHNKKEWHYDVIRKIKKLNEKGFCISVMIDTEGSQ 164
           GPACCAIEDLEKLALEGMNIARLNMCHNKKEWHYDVIRKIKKLNEKGFCISVMIDTEGSQ
Sbjct: 61  GPACCAIEDLEKLALEGMNIARLNMCHNKKEWHYDVIRKIKKLNEKGFCISVMIDTEGSQ 120

Query: 165 IHVVDHGAPSSVKVEDGSNWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMAS 224
           IHVVDHGAP SVKVEDGSNWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMAS
Sbjct: 121 IHVVDHGAPFSVKVEDGSNWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMAS 180

Query: 225 FEVMEKIGNDLSCKCTDPGLFLPRAKCSFWRDGRLVMRKHESPTLSSKDWSDIEFGISEG 284
           FEVMEKIGNDLSCKCTDPGLFLPRAKCSFWRDGRLVMRKHESPTLSSKDWSDIEFGISEG
Sbjct: 181 FEVMEKIGNDLSCKCTDPGLFLPRAKCSFWRDGRLVMRKHESPTLSSKDWSDIEFGISEG 240

Query: 285 VDFIALSFVNSADSVKHLKSYLSAKSAKSVRVLAKIESLESLQNLEEIIKASDGIMVARG 344
           VDFIALSFVNSADSVKHLKSYLSAKSAKSVRVLAKIESLESLQNLEEIIKASDGIMVARG
Sbjct: 241 VDFIALSFVNSADSVKHLKSYLSAKSAKSVRVLAKIESLESLQNLEEIIKASDGIMVARG 300

Query: 345 DLGVEIPLEQIPAVQEEITSVCRELNKPVIVASQLLESMVEYPTPTRAEVADVSEVVRQY 404
           DLGVEIPLEQIPAVQEEITSVCRELNKPVIVASQLLESMVEYPTPTRAEVADVSEVVRQY
Sbjct: 301 DLGVEIPLEQIPAVQEEITSVCRELNKPVIVASQLLESMVEYPTPTRAEVADVSEVVRQY 360

Query: 405 ADALMLSGESAIGAYGQKALSVLQIASSRMELWSREENTKHFLPQHQLGVSLHDRIAEEI 464
           ADALML GESAIG+YGQKALSVLQIASSRMELWS EENTKHFLPQHQLGVSLHDRIAEEI
Sbjct: 361 ADALMLCGESAIGSYGQKALSVLQIASSRMELWSGEENTKHFLPQHQLGVSLHDRIAEEI 420

Query: 465 CNSAAELANKLSVDAIFVLTKQGHMASLLSRNRPSPPIFALTDDDSTRMALNLQWGVFPL 524
           CNSAAELANKLSVDAIFVLTKQGHMASLLSRNRPSPPIFALTDDDSTRMALNLQWGVFPL
Sbjct: 421 CNSAAELANKLSVDAIFVLTKQGHMASLLSRNRPSPPIFALTDDDSTRMALNLQWGVFPL 480

Query: 525 RIDLSDDIEANISRGIELVKSKGLVKQGDFVLMVSEISPARAASMASQSIQLKSIV 581
           RIDLSDDIEANISRGIELVKSKGLVKQGDFVLMVSEIS ARAASMASQSIQLKSIV
Sbjct: 481 RIDLSDDIEANISRGIELVKSKGLVKQGDFVLMVSEISAARAASMASQSIQLKSIV 536

[Close]

BLAST of Carg08103 vs. ExPASy TrEMBL
Match: A0A6J1ILG4 (Pyruvate kinase OS=Cucurbita maxima OX=3661 GN=LOC111475842 PE=3 SV=1)

HSP 1 Score: 1016.5 bits (2627), Expect = 4.3e-293
Identity = 522/536 (97.39%), Postives = 527/536 (98.32%), Query Frame = 0


Query: 45  MKGSVVTGVKAAMQVDLEDDERLKRLDGLEATLGLDVVAERELKEKGFLGMRKTKFVCTI 104
           MKG VVTGVKA MQVDLEDDERLKRLDGLEATLGLDVVAERELKEKGFLGMRKTKFVCTI
Sbjct: 1   MKGRVVTGVKATMQVDLEDDERLKRLDGLEATLGLDVVAERELKEKGFLGMRKTKFVCTI 60

Query: 105 GPACCAIEDLEKLALEGMNIARLNMCHNKKEWHYDVIRKIKKLNEKGFCISVMIDTEGSQ 164
           GPACCA EDLEKLALEGMNIARLNMCHN KEWHY VIRKIKKLNEKGFCISVMIDTEGSQ
Sbjct: 61  GPACCATEDLEKLALEGMNIARLNMCHNTKEWHYGVIRKIKKLNEKGFCISVMIDTEGSQ 120

Query: 165 IHVVDHGAPSSVKVEDGSNWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMAS 224
           IHVVDHGAPSSVKVEDGSNWLFTAEKFEGSRPFTVQASY GFSEGIQVGDSIVIDGGMAS
Sbjct: 121 IHVVDHGAPSSVKVEDGSNWLFTAEKFEGSRPFTVQASYGGFSEGIQVGDSIVIDGGMAS 180

Query: 225 FEVMEKIGNDLSCKCTDPGLFLPRAKCSFWRDGRLVMRKHESPTLSSKDWSDIEFGISEG 284
           FEVMEKIGNDLSCKCTDPGLFLPRAKCSFWRDGRLV+RKHESPTLSSKDWSDIEFGISEG
Sbjct: 181 FEVMEKIGNDLSCKCTDPGLFLPRAKCSFWRDGRLVVRKHESPTLSSKDWSDIEFGISEG 240

Query: 285 VDFIALSFVNSADSVKHLKSYLSAKSAKSVRVLAKIESLESLQNLEEIIKASDGIMVARG 344
           VDFIALSFVNSADSVKHLKSYLSAKSAKSVRVLAKIESLESLQNLEEII+ASDGIMVARG
Sbjct: 241 VDFIALSFVNSADSVKHLKSYLSAKSAKSVRVLAKIESLESLQNLEEIIEASDGIMVARG 300

Query: 345 DLGVEIPLEQIPAVQEEITSVCRELNKPVIVASQLLESMVEYPTPTRAEVADVSEVVRQY 404
           DLGVEIPLEQIPAVQEEITSVCRELNKPVIVASQLLESMVEYPTPTRAEVADVSE VRQY
Sbjct: 301 DLGVEIPLEQIPAVQEEITSVCRELNKPVIVASQLLESMVEYPTPTRAEVADVSEAVRQY 360

Query: 405 ADALMLSGESAIGAYGQKALSVLQIASSRMELWSREENTKHFLPQHQLGVSLHDRIAEEI 464
           ADALML GESAIG+YGQKALSVLQIASSRMELWSREEN KHFLPQHQLGVSLHDRIAEEI
Sbjct: 361 ADALMLCGESAIGSYGQKALSVLQIASSRMELWSREENRKHFLPQHQLGVSLHDRIAEEI 420

Query: 465 CNSAAELANKLSVDAIFVLTKQGHMASLLSRNRPSPPIFALTDDDSTRMALNLQWGVFPL 524
           CNSAAELANKLSVDAIFVLTKQGHMASLLSRNRPSPPIFALT++DSTRMALNLQWGVFPL
Sbjct: 421 CNSAAELANKLSVDAIFVLTKQGHMASLLSRNRPSPPIFALTNEDSTRMALNLQWGVFPL 480

Query: 525 RIDLSDDIEANISRGIELVKSKGLVKQGDFVLMVSEISPARAASMASQSIQLKSIV 581
           RIDLSDDIEANISRGIELVKSKGLVKQGDFVLMVSEISPARAASMASQSIQLKSIV
Sbjct: 481 RIDLSDDIEANISRGIELVKSKGLVKQGDFVLMVSEISPARAASMASQSIQLKSIV 536

[Close]

BLAST of Carg08103 vs. ExPASy TrEMBL
Match: A0A0A0KSY4 (Pyruvate kinase OS=Cucumis sativus OX=3659 GN=Csa_4G000560 PE=3 SV=1)

HSP 1 Score: 1010.0 bits (2610), Expect = 4.0e-291
Identity = 517/582 (88.83%), Postives = 548/582 (94.16%), Query Frame = 0


Query: 1   MAEVSAMMIRACCNFVAKTSVLDVKRSDSRLSVIG-RTVWCDRGRMKGSVVTGVKAAMQV 60
           MAEV+AMMIRA CNF+ +  VLDVKR   R+ V+G RTVWC R RMKG VV GVKA MQV
Sbjct: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGRRTVWCSRRRMKGRVVNGVKATMQV 60

Query: 61  DLEDDERLKRLDGLEATLGLDVVAERELKEKGFLGMRKTKFVCTIGPACCAIEDLEKLAL 120
           DLED+ERLKRL+GLEATLGLD+VAERELKEKGFLGMRKTK VCT+GPAC  I DLEKLAL
Sbjct: 61  DLEDEERLKRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTVGPACSGIADLEKLAL 120

Query: 121 EGMNIARLNMCHNKKEWHYDVIRKIKKLN-EKGFCISVMIDTEGSQIHVVDHGAPSSVKV 180
           EGMNIARLNMCHN  EWH D+I+KIKKLN EKGFCISVMID EGSQIHVVDHGAPSSVKV
Sbjct: 121 EGMNIARLNMCHNSMEWHCDMIKKIKKLNEEKGFCISVMIDIEGSQIHVVDHGAPSSVKV 180

Query: 181 EDGSNWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMASFEVMEKIGNDLSCK 240
           EDGS WLFTA+KFEGSRPFTVQASYEGFSEGI+VGDSIVIDGGMA+FEV+EKIGNDLSC+
Sbjct: 181 EDGSIWLFTAKKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVIEKIGNDLSCR 240

Query: 241 CTDPGLFLPRAKCSFWRDGRLVMRKHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADS 300
           CTDPGLFLPRAKCSFWRDGRLVMR HESPTLSSKDWSDIEFGISEGVDFIALSFVNSAD 
Sbjct: 241 CTDPGLFLPRAKCSFWRDGRLVMRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300

Query: 301 VKHLKSYLSAKSAKSVRVLAKIESLESLQNLEEIIKASDGIMVARGDLGVEIPLEQIPAV 360
           V+HLK+YLS KSAKS+RVLAKIESLE+LQNLEEI++ASDGIMVARGDLGVEIPLEQIPAV
Sbjct: 301 VRHLKNYLSTKSAKSIRVLAKIESLEALQNLEEIVEASDGIMVARGDLGVEIPLEQIPAV 360

Query: 361 QEEITSVCRELNKPVIVASQLLESMVEYPTPTRAEVADVSEVVRQYADALMLSGESAIGA 420
           QEEIT VCRELNKPVI+ASQLLESMVEYPTPTRAEVADVSE VRQYADALMLSGESAIG+
Sbjct: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420

Query: 421 YGQKALSVLQIASSRMELWSREENTKHFLPQHQLGVSLHDRIAEEICNSAAELANKLSVD 480
           YGQKALSVLQ+ASSRMELWSREEN  +FLPQHQLGVSLHDRIAEEICNSAAELAN+LSVD
Sbjct: 421 YGQKALSVLQMASSRMELWSREENRNNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480

Query: 481 AIFVLTKQGHMASLLSRNRPSPPIFALTDDDSTRMALNLQWGVFPLRIDLSDDIEANISR 540
           AIFVLT QGHMASLLSRNRP PPIFALTDDDSTRMALNLQWGVFPLRIDLS+DIEANISR
Sbjct: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540

Query: 541 GIELVKSKGLVKQGDFVLMVSEISPARAASMASQSIQLKSIV 581
           GIE+VKSKGLVKQGD VL+VSEISPARAASMASQSIQLK+IV
Sbjct: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTIV 582

[Close]

BLAST of Carg08103 vs. TAIR 10
Match: AT3G22960.1 (Pyruvate kinase family protein )

HSP 1 Score: 595.9 bits (1535), Expect = 3.5e-170
Identity = 294/487 (60.37%), Postives = 369/487 (75.77%), Query Frame = 0


Query: 79  LDVVAERELKEKGFLGMRKTKFVCTIGPACCAIEDLEKLALEGMNIARLNMCHNKKEWHY 138
           +D V E ELKE GF   R+TK +CTIGPA C  E LE LA+ GMN+ARLNMCH  ++WH 
Sbjct: 100 VDTVTEAELKENGFRSTRRTKLICTIGPATCGFEQLEALAVGGMNVARLNMCHGTRDWHR 159

Query: 139 DVIRKIKKLN-EKGFCISVMIDTEGSQIHVVDHGAPSSVKVEDGSNWLFTAEKFEGSRP- 198
            VIR +++LN EKGF +++M+DTEGS+IH+ D G  +S K EDG  W FT   F+ SRP 
Sbjct: 160 GVIRSVRRLNEEKGFAVAIMMDTEGSEIHMGDLGGEASAKAEDGEVWTFTVRAFDSSRPE 219

Query: 199 FTVQASYEGFSEGIQVGDSIVIDGGMASFEVMEKIGNDLSCKCTDPGLFLPRAKCSFWRD 258
            T+  SY+GF+E ++VGD +++DGGM  FEV+EKIG D+ C CTDPGL LPRA  +FWRD
Sbjct: 220 RTISVSYDGFAEDVRVGDELLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRD 279

Query: 259 GRLVMRKHES-PTLSSKDWSDIEFGISEGVDFIALSFVNSADSVKHLKSYLSAKS-AKSV 318
           G LV  ++   PT+SSKDW DI+FGI+EGVDFIA+SFV SA+ + HLKSYL+A+S    +
Sbjct: 280 GSLVRERNAMLPTISSKDWLDIDFGIAEGVDFIAVSFVKSAEVINHLKSYLAARSRGGEI 339

Query: 319 RVLAKIESLESLQNLEEIIKASDGIMVARGDLGVEIPLEQIPAVQEEITSVCRELNKPVI 378
            V+AKIES++SL NLEEII ASDG MVARGDLG +IPLEQ+PA Q+ I  VCR LNKPVI
Sbjct: 340 GVIAKIESIDSLTNLEEIILASDGAMVARGDLGAQIPLEQVPAAQQRIVQVCRALNKPVI 399

Query: 379 VASQLLESMVEYPTPTRAEVADVSEVVRQYADALMLSGESAIGAYGQKALSVLQIASSRM 438
           VASQLLESM+EYPTPTRAEVADVSE VRQ +DALMLSGESA+G +  KAL+VL+  S R+
Sbjct: 400 VASQLLESMIEYPTPTRAEVADVSEAVRQRSDALMLSGESAMGQFPDKALTVLRTVSLRI 459

Query: 439 ELWSREENTKHFLPQHQLGVSLHDRIAEEICNSAAELANKLSVDAIFVLTKQGHMASLLS 498
           E W REE     +P   +G S  D+I+EEICNSAA++AN L VDA+FV T  GHMASL+S
Sbjct: 460 ERWWREEKRHESVPLQAIGSSFSDKISEEICNSAAKMANNLGVDAVFVYTTSGHMASLVS 519

Query: 499 RNRPSPPIFALTDDDSTRMALNLQWGVFPLRIDLSDDIEANISRGIELVKSKGLVKQGDF 558
           R RP  PIFA T   S R  LNLQWG+ P R+  SDD+E+N+++   L+KS+G++K GD 
Sbjct: 520 RCRPDCPIFAFTTTTSVRRRLNLQWGLIPFRLSFSDDMESNLNKTFSLLKSRGMIKSGDL 579

Query: 559 VLMVSEI 562
           V+ VS++
Sbjct: 580 VIAVSDM 586

[Close]

BLAST of Carg08103 vs. TAIR 10
Match: AT5G52920.1 (plastidic pyruvate kinase beta subunit 1 )

HSP 1 Score: 314.7 bits (805), Expect = 1.6e-85
Identity = 185/486 (38.07%), Postives = 280/486 (57.61%), Query Frame = 0


Query: 96  RKTKFVCTIGPACCAIEDLEKLALEGMNIARLNMCHNKKEWHYDVIRKIKKLN--EKGFC 155
           RKTK VCT+GP+    E + KLA  GMN+AR+NM H     H  VI  +K+ N   K   
Sbjct: 110 RKTKIVCTVGPSTNTREMIWKLAEAGMNVARMNMSHGDHASHKKVIDLVKEYNAQTKDNT 169

Query: 156 ISVMIDTEGSQIHVVDHGAPSSVKVEDGSNWLFTAEKFEGSRPFTVQASYEGFSEGIQVG 215
           I++M+DT+G ++   D   P  + ++ G  + FT E+   S P  V  +Y+ F   ++ G
Sbjct: 170 IAIMLDTKGPEVRSGD--LPQPIMLDPGQEFTFTIER-GVSTPSCVSVNYDDFVNDVEAG 229

Query: 216 DSIVIDGGMASFEVMEKIGNDLSCKCTDPGLFLPRAKCSFWRDGRLVMRKHESPTLSSKD 275
           D +++DGGM SF V  K  + + C+  D G    R      R   +  +    P+++ KD
Sbjct: 230 DMLLVDGGMMSFMVKSKTKDSVKCEVVDGGELKSR------RHLNVRGKSATLPSITEKD 289

Query: 276 WSDIEFGISEGVDFIALSFVNSADSVKHLKSYLSAKSAKSVRVLAKIESLESLQNLEEII 335
           W DI+FG+   VDF A+SFV  A  V  LK YL   S   + V+ KIES +S+ NL  II
Sbjct: 290 WEDIKFGVENKVDFYAVSFVKDAQVVHELKKYLQ-NSGADIHVIVKIESADSIPNLHSII 349

Query: 336 KASDGIMVARGDLGVEIPLEQIPAVQEEITSVCRELNKPVIVASQLLESMVEYPTPTRAE 395
            ASDG MVARGDLG E+P+E++P +QEEI ++CR + K VIVA+ +LESM+ +PTPTRAE
Sbjct: 350 TASDGAMVARGDLGAELPIEEVPILQEEIINLCRSMGKAVIVATNMLESMIVHPTPTRAE 409

Query: 396 VADVSEVVRQYADALMLSGESAIGAYGQKALSVLQIASSRMELWSREENTKHFLPQHQLG 455
           V+D++  VR+ ADA+MLSGE+A G +  KA  V+   + R E       T   +P + LG
Sbjct: 410 VSDIAIAVREGADAVMLSGETAHGKFPLKAAGVMHTVALRTE----ATITSGEMPPN-LG 469

Query: 456 VSLHDRIAEEICNSAAELANKLSVDAIFVLTKQGHMASLLSRNRPSPPIFALTDDDSTRM 515
            +  + ++E     A  ++N L    + V T+ G MA LLS  RPS  I+A T++   + 
Sbjct: 470 QAFKNHMSEMFAYHATMMSNTLGTSTV-VFTRTGFMAILLSHYRPSGTIYAFTNEKKIQQ 529

Query: 516 ALNLQWGVFPLRIDLSDDIEANISRGIELVKSKGLVKQGDFVLMVSEISPARAASMASQS 575
            L L  GV P+ ++ +DD E   +  +  +  +G+VK+G+ + +V   +     S ++ +
Sbjct: 530 RLALYQGVCPIYMEFTDDAEETFANALATLLKQGMVKKGEEIAIVQSGTQPIWRSQSTHN 579

Query: 576 IQLKSI 580
           IQ++ +
Sbjct: 590 IQVRKV 579

[Close]

BLAST of Carg08103 vs. TAIR 10
Match: AT1G32440.1 (plastidial pyruvate kinase 3 )

HSP 1 Score: 306.6 bits (784), Expect = 4.2e-83
Identity = 189/488 (38.73%), Postives = 280/488 (57.38%), Query Frame = 0


Query: 96  RKTKFVCTIGPACCAIEDLEKLALEGMNIARLNMCHNKKEWHYDVIRKIKKLNE--KGFC 155
           RKTK VCTIGP+  + E + KLA  GMN+ARLNM H     H   I  +K+ N       
Sbjct: 99  RKTKIVCTIGPSSSSREMIWKLAEAGMNVARLNMSHGDHASHQITIDLVKEYNSLFVDKA 158

Query: 156 ISVMIDTEGSQIHVVDHGAPSSVKVEDGSNWLFTAEKFEGSRPFTVQASYEGFSEGIQVG 215
           I++M+DT+G ++   D   P  + +E+G  + FT ++    +  TV  +Y+ F   ++VG
Sbjct: 159 IAIMLDTKGPEVRSGD--VPQPIFLEEGQEFNFTIKRGVSLKD-TVSVNYDDFVNDVEVG 218

Query: 216 DSIVIDGGMASFEVMEKIGNDLSCKCTDPGLFLPRAKCSFWRDGRLVMRKHESPTLSSKD 275
           D +++DGGM S  V  K  + + C   D G    R      R   +  +    P+++ KD
Sbjct: 219 DILLVDGGMMSLAVKSKTSDLVKCVVIDGGELQSR------RHLNVRGKSATLPSITDKD 278

Query: 276 WSDIEFGISEGVDFIALSFVNSADSVKHLKSYLSAKSAKSVRVLAKIESLESLQNLEEII 335
           W DI+FG+   VDF A+SFV  A  V  LK+YL   SA  + V+ KIES +S++NL  II
Sbjct: 279 WEDIKFGVDNQVDFYAVSFVKDAKVVHELKNYLKTCSA-DISVIVKIESADSIKNLPSII 338

Query: 336 KASDGIMVARGDLGVEIPLEQIPAVQEEITSVCRELNKPVIVASQLLESMVEYPTPTRAE 395
            A DG MVARGDLG E+P+E++P +QEEI   CR ++KPVIVA+ +LESM+ +PTPTRAE
Sbjct: 339 SACDGAMVARGDLGAELPIEEVPLLQEEIIRRCRSIHKPVIVATNMLESMINHPTPTRAE 398

Query: 396 VADVSEVVRQYADALMLSGESAIGAYGQKALSVLQIASSRME--LWSREENTKHFLPQHQ 455
           V+D++  VR+ ADA+MLSGE+A G +  KA++V+   + R E  L  R   ++    +  
Sbjct: 399 VSDIAIAVREGADAIMLSGETAHGKFPLKAVNVMHTVALRTEASLPVRTSASRTTAYKGH 458

Query: 456 LGVSLHDRIAEEICNSAAELANKLSVDAIFVLTKQGHMASLLSRNRPSPPIFALTDDDST 515
           +G        +     A+ +AN LS   + V T+ G MA LLS  RPS  IFA T+    
Sbjct: 459 MG--------QMFAFHASIMANTLS-SPLIVFTRTGSMAVLLSHYRPSATIFAFTNQRRI 518

Query: 516 RMALNLQWGVFPLRIDLSDDIEANISRGIELVKSKGLVKQGDFVLMVSEISPARAASMAS 575
              L L  GV P+ ++ SDD E   +R ++L++ + ++K+G  V +V   S       ++
Sbjct: 519 MQRLALYQGVMPIYMEFSDDAEDTYARSLKLLQDENMLKEGQHVTLVQSGSQPIWREEST 567

Query: 576 QSIQLKSI 580
             IQ++ I
Sbjct: 579 HLIQVRKI 567

[Close]

BLAST of Carg08103 vs. TAIR 10
Match: AT3G04050.1 (Pyruvate kinase family protein )

HSP 1 Score: 225.7 bits (574), Expect = 9.5e-59
Identity = 166/502 (33.07%), Postives = 254/502 (50.60%), Query Frame = 0


Query: 97  KTKFVCTIGPACCAIEDLEKLALEGMNIARLNMCHNKKEWHYDVIRKIKKLNEKGFCI-- 156
           KTK VCT+GPA  ++E +EKL   GMN+AR N  H    +H + +  ++   E   CI  
Sbjct: 17  KTKIVCTLGPASRSVEMVEKLLKAGMNVARFNFSHGSHSYHQETLDNLRTAME-NTCIPC 76

Query: 157 SVMIDTEGSQIHV--VDHGAPSSVKVEDGSNWLFTAEKFEGSRPFTVQASYEGFSEGIQV 216
           +VM+DT+G +I    +  G P  V++  G     + +        T+  SY+  +E ++ 
Sbjct: 77  AVMLDTKGPEIRTGFLKEGKP--VELIQGQEITISTDYTMEGDSNTISMSYKKLAEDLKS 136

Query: 217 GDSIVIDGGMASFEVMEKIGNDLSCKCTDPGLFLPRAKCSFWRDGRLVMRKH-------- 276
           GD I+   G  S  V       LSC   D  L L RA+C       L  RK+        
Sbjct: 137 GDVILCSDGTISLTV-------LSC---DKNLGLVRARCE--NSAVLGERKNVNLPGIVV 196

Query: 277 ESPTLSSKDWSDI-EFGISEGVDFIALSFVNSADSVKHLKSYLSAKSAKSVRVLAKIESL 336
           + PTL+ KD  DI ++G+   +D IALSFV     +  ++  L  ++AKS+ +++K+E+ 
Sbjct: 197 DLPTLTEKDQEDILQWGVPNKIDIIALSFVRKGSDLVEVRKLL-GENAKSIMLMSKVENQ 256

Query: 337 ESLQNLEEIIKASDGIMVARGDLGVEIPLEQIPAVQEEITSVCRELNKPVIVASQLLESM 396
           E + N ++I++ SD  MVARGDLG+EIP+E++   Q+ +      L KP++ A+Q+LESM
Sbjct: 257 EGVMNFDKILEYSDAFMVARGDLGMEIPIEKMFLAQKMMIQKANALGKPIVTATQMLESM 316

Query: 397 VEYPTPTRAEVADVSEVVRQYADALMLSGESAIGAYGQKALSVLQIASSRMELWSREENT 456
            + P PTRAE  DV+  V    D +MLSGE+A GA+ + A+  +       E +  + +T
Sbjct: 317 TKSPRPTRAEATDVANAVLDGTDCVMLSGETAAGAHPETAVLTMSRICKEAEDFI-DYDT 376

Query: 457 KHFLPQHQLGVSLHDRIAEEICNSAAELANKLSVDAIFVLTKQGHMASLLSRNRPSPPIF 516
            H   + Q  VSL     E +  SA   A  L   AI VLTK G+   L+++ RPS PI 
Sbjct: 377 MH--KKIQDIVSLPLSPIESLAASAVSTARSLCAAAIVVLTKGGYTVELVAKYRPSVPIL 436

Query: 517 ALTDDDSTRMALNLQW-----------------GVFPL-------RIDLSDDIEANISRG 562
           ++   + TR   + +W                 G+ P+       R    D  E  I   
Sbjct: 437 SVIVPEITRTD-DFEWSCSESAAHVARRGLIYRGIVPVMATGASARSSNKDSTEETIQFA 496

[Close]

BLAST of Carg08103 vs. TAIR 10
Match: AT5G63680.1 (Pyruvate kinase family protein )

HSP 1 Score: 221.9 bits (564), Expect = 1.4e-57
Identity = 154/494 (31.17%), Postives = 252/494 (51.01%), Query Frame = 0


Query: 97  KTKFVCTIGPACCAIEDLEKLALEGMNIARLNMCHNKKEWHYDVIRKIK-KLNEKGFCIS 156
           KTK VCT+GPA  ++  +EKL   GMN+AR N  H   E+H + +  ++  +   G   +
Sbjct: 21  KTKIVCTLGPASRSVTMIEKLLKAGMNVARFNFSHGSHEYHQETLDNLRTAMQNTGILAA 80

Query: 157 VMIDTEGSQIHVVDHGAPSSVKVEDGSNWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDS 216
           VM+DT+G +I        + +++++G     T +        T+  SY+     ++ G++
Sbjct: 81  VMLDTKGPEIRTGFLKDGNPIQLKEGQEITITTDYDIKGDEKTISMSYKKLPVDVKPGNT 140

Query: 217 IVIDGGMASFEVM--EKIGNDLSCKCTDPGLFLPRAKCSFWRDGRLVMRKHESPTLSSKD 276
           I+   G  S  V+  +     + C+C +  +   R   +    G +V    + PTL+ KD
Sbjct: 141 ILCADGSISLAVVSCDPNAGTVICRCENTAMLGERKNVNL--PGVVV----DLPTLTDKD 200

Query: 277 WSDI-EFGISEGVDFIALSFVNSADSVKHLKSYLSAKSAKSVRVLAKIESLESLQNLEEI 336
             DI ++G+   +D IALSFV     + +++  L + S KS+ +++K+E+ E + N +EI
Sbjct: 201 VEDILKWGVPNNIDMIALSFVRKGSDLVNVRKVLGSHS-KSIMLMSKVENQEGVLNFDEI 260

Query: 337 IKASDGIMVARGDLGVEIPLEQIPAVQEEITSVCRELNKPVIVASQLLESMVEYPTPTRA 396
           ++ +D  MVARGDLG+EIP+E+I   Q+ +   C    KPV+ A+Q+LESM++ P PTRA
Sbjct: 261 LRETDAFMVARGDLGMEIPIEKIFLAQKMMIYKCNLAGKPVVTATQMLESMIKSPRPTRA 320

Query: 397 EVADVSEVVRQYADALMLSGESAIGAYGQKALSVLQIASSRMELWSREENT--KHFLPQH 456
           E  DV+  V    D +MLSGESA GAY + A+  +       E  S + NT  K  +   
Sbjct: 321 EATDVANAVLDGTDCVMLSGESAAGAYPEIAVKTMAKICIEAES-SLDYNTIFKEMIRAT 380

Query: 457 QLGVSLHDRIAEEICNSAAELANKLSVDAIFVLTKQGHMASLLSRNRPSPPIFAL----- 516
            L +S      E + +SA   ANK     I VLT+ G  A L+++ RP+ PI ++     
Sbjct: 381 PLPMS----TLESLASSAVRTANKAKAKLIIVLTRGGTTAKLVAKYRPAVPILSVVVPVF 440

Query: 517 ---------TDDDSTRMALNLQWGVFPL------RIDLSDDIEANISRGIELVKSKGLVK 565
                    +D+   R +L +  G+ P+      +   S+  E  I   ++    KGL  
Sbjct: 441 TSDTFNWSCSDESPARHSL-IYRGLIPVLGEGSAKATDSESTEEIIESALKSATEKGLCN 500

The following BLAST results are available for this feature:

BLAST of Carg08103 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6600268.1	0.0e+00	100.00	Plastidial pyruvate kinase 1, chloroplastic, partial [Cucurbita argyrosperma sub...	[more]
XP_022942487.1	0.0e+00	98.79	plastidial pyruvate kinase 1, chloroplastic-like isoform X1 [Cucurbita moschata]	[more]
XP_022975726.1	0.0e+00	97.41	plastidial pyruvate kinase 1, chloroplastic-like isoform X1 [Cucurbita maxima]	[more]
XP_023515955.1	0.0e+00	97.07	plastidial pyruvate kinase 1, chloroplastic-like isoform X1 [Cucurbita pepo subs...	[more]
XP_022942488.1	7.8e-297	98.88	plastidial pyruvate kinase 1, chloroplastic-like isoform X2 [Cucurbita moschata]	[more]

BLAST of Carg08103 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LIK0	4.9e-169	60.37	Plastidial pyruvate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=P...	[more]
Q43117	6.4e-169	59.06	Pyruvate kinase isozyme A, chloroplastic OS=Ricinus communis OX=3988 PE=1 SV=1	[more]
Q40545	8.1e-164	57.84	Pyruvate kinase isozyme A, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
Q40546	8.0e-87	39.30	Pyruvate kinase isozyme G, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
Q9FLW9	2.2e-84	38.07	Plastidial pyruvate kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PKP2 PE=1 SV=1	[more]

BLAST of Carg08103 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FQD7	0.0e+00	98.79	Pyruvate kinase OS=Cucurbita moschata OX=3662 GN=LOC111447508 PE=3 SV=1	[more]
A0A6J1IF03	0.0e+00	97.41	Pyruvate kinase OS=Cucurbita maxima OX=3661 GN=LOC111475842 PE=3 SV=1	[more]
A0A6J1FUY6	3.8e-297	98.88	Pyruvate kinase OS=Cucurbita moschata OX=3662 GN=LOC111447508 PE=3 SV=1	[more]
A0A6J1ILG4	4.3e-293	97.39	Pyruvate kinase OS=Cucurbita maxima OX=3661 GN=LOC111475842 PE=3 SV=1	[more]
A0A0A0KSY4	4.0e-291	88.83	Pyruvate kinase OS=Cucumis sativus OX=3659 GN=Csa_4G000560 PE=3 SV=1	[more]

BLAST of Carg08103 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G22960.1	3.5e-170	60.37	Pyruvate kinase family protein	[more]
AT5G52920.1	1.6e-85	38.07	plastidic pyruvate kinase beta subunit 1	[more]
AT1G32440.1	4.2e-83	38.73	plastidial pyruvate kinase 3	[more]
AT3G04050.1	9.5e-59	33.07	Pyruvate kinase family protein	[more]
AT5G63680.1	1.4e-57	31.17	Pyruvate kinase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001697	Pyruvate kinase	PRINTS	PR01050	PYRUVTKNASE	coord: 152..168 score: 27.42 coord: 285..299 score: 47.28 coord: 343..367 score: 48.92 coord: 393..411 score: 49.07 coord: 368..392 score: 58.28 coord: 412..428 score: 35.38 coord: 316..342 score: 50.88
IPR001697	Pyruvate kinase	TIGRFAM	TIGR01064	TIGR01064	coord: 96..565 e-value: 2.5E-121 score: 403.4
IPR001697	Pyruvate kinase	PANTHER	PTHR11817	PYRUVATE KINASE	coord: 39..579
IPR015806	Pyruvate kinase, insert domain superfamily	GENE3D	2.40.33.10		coord: 163..267 e-value: 4.5E-139 score: 466.0
IPR036918	Pyruvate kinase, C-terminal domain superfamily	GENE3D	3.40.1380.20		coord: 435..561 e-value: 4.5E-139 score: 466.0
IPR036918	Pyruvate kinase, C-terminal domain superfamily	SUPERFAMILY	52935	PK C-terminal domain-like	coord: 458..561
IPR015793	Pyruvate kinase, barrel	PFAM	PF00224	PK	coord: 95..430 e-value: 1.1E-84 score: 284.3
IPR040442	Pyruvate kinase-like domain superfamily	GENE3D	3.20.20.60		coord: 96..434 e-value: 4.5E-139 score: 466.0
IPR015795	Pyruvate kinase, C-terminal	PFAM	PF02887	PK_C	coord: 462..562 e-value: 7.7E-21 score: 74.5
None	No IPR available	PANTHER	PTHR11817:SF21	PYRUVATE KINASE	coord: 39..579
IPR018209	Pyruvate kinase, active site	PROSITE	PS00110	PYRUVATE_KINASE	coord: 314..326
IPR015813	Pyruvate/Phosphoenolpyruvate kinase-like domain superfamily	SUPERFAMILY	51621	Phosphoenolpyruvate/pyruvate domain	coord: 90..435
IPR011037	Pyruvate kinase-like, insert domain superfamily	SUPERFAMILY	50800	PK beta-barrel domain-like	coord: 165..252

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg08103-RA	Carg08103-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006096	glycolytic process
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0016301	kinase activity
molecular_function	GO:0000287	magnesium ion binding
molecular_function	GO:0030955	potassium ion binding
molecular_function	GO:0004743	pyruvate kinase activity
molecular_function	GO:0003824	catalytic activity