Carg08054 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg08054
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionPentatricopeptide repeat-containing protein
LocationCarg_Chr04: 1995187 .. 1997487 (+)
RNA-Seq ExpressionCarg08054
SyntenyCarg08054
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAATGAAATGAAAAATAACCCACGATGTGGATGGGCAGGATTGTGGTTAAGCCCTAACAAAACCTCCGACGTATCACGCACCTCAGGACAAGTGATTTTCGAGAGAAATGAATCGTCGGAGTTTACTCTCGAGAGCGTCGGCAGGTTTGCGGCATCTCTGTACTTCAACCGCCGAGTCGAAGCGCGGTCCTGTGAATGATCAGCAGCGGCTATACCCGAGGCTGTCGAAGTTGGGTGCCACCGGCGGTAGCGTGGCGCAGACATTGAACCAGTACATTATGGAGGGAAAGATCGTCAAAAAATATGAGCTCGAGAGATGCATAAAGGAGCTCCGGAAGTACCGTAGATACCACCACGCCCTTCAGGTTTTTTCCTTAAACACATAGGTTCGTTCACTGTAGGAGAAATATAAATGCACAAAATTACTGCTGCTTTTCATTTGTTTTTCAAATTCTATTGCCAATTGTTGATTGTATTCTTCCTTTCGCTGCTAGATCATAAATTTTTTGTAAACGAAACATTCATGAAATATAAAGGAAATGACAAAAACCTTGTGAAGAATTTACGATAAAAAATCATTGGATCTGGTAGCTATGAAAAAGCACTCTGGAGGGAGAACTAGCCAAAAAGTTGCTGTAATTGCAGGAGACCATAAGATCTTAGCTCATCCATTGCATTTGCTTCGGTTGTTGCAGATAATGGAATGGATGGAGATGAGGAAAATCAACTACTCATTCACTGACTACGCGCTGCGTTTAGATCTTATATCGAAAGTTAAAGGAATTGCTGCTGCGGAGAATTATTTCTGTGATCTGTCGCCGTCTGCGAAGAATCGATTTACTTATGGAGCTCTTTTGAATTGCTATTGCAAGGAATTGATGGAGGAAAAGGCATTGGCTCTTTCTAAGAAGATAGATGAGTTGAAGTTTGCTTCCAATTTGTCCTTTAACAATCTTATGACCATGTATATGAGAATGGATCAACCCGAGAAAGTACCTCCTCTTATAGATGAAATGAAGCAGAGAGGGATTTTTCTTAGCACGTACACATACAATGTTTGGATGAACAGTTGTGCTTCCCTGAATGGCGTTGGAAAAGTTGAAGAAATTCTCGAGGAGATGAAAAACGAAGACAGAAACAAATTTGATTGGACGACGTTTTCGAACTTGGCTGCTATCTATGTTAAGGCAGGACAGCTCGAGAAAGCTGAATTAGCTCTTAAGAAGGTAGAGAACGAGATCAAATCGAATAAGCAGCAGGATCGTCTAGCGTACCATTTCTTGATAAGCCTTTATGCATCGACGTCGAATCGGAGTGAGGTGTATAGGATATGGAATGCACTGAAATCAGTTTATCCAATGACAAATAACATGAGTTATCTCGTCATGCTTCAGGCTCTAAGCAAACTAAAGGATTTCGAGGGTCTTAAAAGCACTTATAAGGAATGGGAATCTAGTTGCTCGAGCTTTGATTTGCGGTTAGCGGATGTTACGATCGGGGCTTACCTACGACAGGACATGTACAAAGATGCTGCATTGGTCTTTGAGGATGCTATTAAGAGAAGTAAAGGACCTTTCTTTAGGGCTCGAGAAATGTTCATGATTTACTTCTTGAAGTTCAAGCAAGTCGATTTGGCACTCAGTCATTTGGAATCAGCTATATCGGAAAGCATGGACGATGAATGGCATCCATCACCGGCGATGGCGAATGCTTTTCTGATGTACTTTGAGGAAGAGAAAGACGTTGAAGGCGCCGAAGATTTTGCCAGGATTTTGAAGAGATTTAAGTGTCTCGACGCTAGTGCATACCATCTATTGCTCAAGACCTATGCAGCTGCAGGAAAACGAGCCCCCGACATGCGACAAAGATTGATAGAAGACAACATTGAAGTAAGTAGTGAGCTTGAGGAGTTGTTAAGTAGACAACATTGAGACAAGTAGGAGGAAAATCCATGGGATGTTTTTTTTTTTATCTGATCTTTATAAGCTGATCCACTTCATTCCTTTTCTCCTAAACGAAATTTGTATTTTCGAGCATTAGTCTATTTTCATTTTCTTGATAAGCTGATGGTGCACAACTTGTAGAAGTTCCCTTGGTTTGTGGTAGACTTGGACCGGGTTTTTGTCACCACGTTATCAGCATCGAAGAAGCCAGGGCTGGCAAGATAAGTTCACGTGGCCACACCCTAAACCAATCAATAAGCTTTTTTATTTGATTGAAAGGAAAAATTACTGCTTATTAGCTTAGCTAGCCTTTGGGAGAATCAAGTCATTTAGCCAGTTCATATGAATATATCAATT

mRNA sequence

AAAATGAAATGAAAAATAACCCACGATGTGGATGGGCAGGATTGTGGTTAAGCCCTAACAAAACCTCCGACGTATCACGCACCTCAGGACAAGTGATTTTCGAGAGAAATGAATCGTCGGAGTTTACTCTCGAGAGCGTCGGCAGGTTTGCGGCATCTCTGTACTTCAACCGCCGAGTCGAAGCGCGGTCCTGTGAATGATCAGCAGCGGCTATACCCGAGGCTGTCGAAGTTGGGTGCCACCGGCGGTAGCGTGGCGCAGACATTGAACCAGTACATTATGGAGGGAAAGATCGTCAAAAAATATGAGCTCGAGAGATGCATAAAGGAGCTCCGGAAGTACCGTAGATACCACCACGCCCTTCAGATAATGGAATGGATGGAGATGAGGAAAATCAACTACTCATTCACTGACTACGCGCTGCGTTTAGATCTTATATCGAAAGTTAAAGGAATTGCTGCTGCGGAGAATTATTTCTGTGATCTGTCGCCGTCTGCGAAGAATCGATTTACTTATGGAGCTCTTTTGAATTGCTATTGCAAGGAATTGATGGAGGAAAAGGCATTGGCTCTTTCTAAGAAGATAGATGAGTTGAAGTTTGCTTCCAATTTGTCCTTTAACAATCTTATGACCATGTATATGAGAATGGATCAACCCGAGAAAGTACCTCCTCTTATAGATGAAATGAAGCAGAGAGGGATTTTTCTTAGCACGTACACATACAATGTTTGGATGAACAGTTGTGCTTCCCTGAATGGCGTTGGAAAAGTTGAAGAAATTCTCGAGGAGATGAAAAACGAAGACAGAAACAAATTTGATTGGACGACGTTTTCGAACTTGGCTGCTATCTATGTTAAGGCAGGACAGCTCGAGAAAGCTGAATTAGCTCTTAAGAAGGTAGAGAACGAGATCAAATCGAATAAGCAGCAGGATCGTCTAGCGTACCATTTCTTGATAAGCCTTTATGCATCGACGTCGAATCGGAGTGAGGTGTATAGGATATGGAATGCACTGAAATCAGTTTATCCAATGACAAATAACATGAGTTATCTCGTCATGCTTCAGGCTCTAAGCAAACTAAAGGATTTCGAGGGTCTTAAAAGCACTTATAAGGAATGGGAATCTAGTTGCTCGAGCTTTGATTTGCGGTTAGCGGATGTTACGATCGGGGCTTACCTACGACAGGACATGTACAAAGATGCTGCATTGGTCTTTGAGGATGCTATTAAGAGAAGTAAAGGACCTTTCTTTAGGGCTCGAGAAATGTTCATGATTTACTTCTTGAAGTTCAAGCAAGTCGATTTGGCACTCAGTCATTTGGAATCAGCTATATCGGAAAGCATGGACGATGAATGGCATCCATCACCGGCGATGGCGAATGCTTTTCTGATGTACTTTGAGGAAGAGAAAGACGTTGAAGGCGCCGAAGATTTTGCCAGGATTTTGAAGAGATTTAAGTGTCTCGACGCTAGTGCATACCATCTATTGCTCAAGACCTATGCAGCTGCAGGAAAACGAGCCCCCGACATGCGACAAAGATTGATAGAAGACAACATTGAAGTAAGTAGTGAGCTTGAGGAGTTGTTAAGTAGACAACATTGAGACAAGTAGGAGGAAAATCCATGGGATGTTTTTTTTTTTATCTGATCTTTATAAGCTGATCCACTTCATTCCTTTTCTCCTAAACGAAATTTGTATTTTCGAGCATTAGTCTATTTTCATTTTCTTGATAAGCTGATGGTGCACAACTTGTAGAAGTTCCCTTGGTTTGTGGTAGACTTGGACCGGGTTTTTGTCACCACGTTATCAGCATCGAAGAAGCCAGGGCTGGCAAGATAAGTTCACGTGGCCACACCCTAAACCAATCAATAAGCTTTTTTATTTGATTGAAAGGAAAAATTACTGCTTATTAGCTTAGCTAGCCTTTGGGAGAATCAAGTCATTTAGCCAGTTCATATGAATATATCAATT

Coding sequence (CDS)

ATGAATCGTCGGAGTTTACTCTCGAGAGCGTCGGCAGGTTTGCGGCATCTCTGTACTTCAACCGCCGAGTCGAAGCGCGGTCCTGTGAATGATCAGCAGCGGCTATACCCGAGGCTGTCGAAGTTGGGTGCCACCGGCGGTAGCGTGGCGCAGACATTGAACCAGTACATTATGGAGGGAAAGATCGTCAAAAAATATGAGCTCGAGAGATGCATAAAGGAGCTCCGGAAGTACCGTAGATACCACCACGCCCTTCAGATAATGGAATGGATGGAGATGAGGAAAATCAACTACTCATTCACTGACTACGCGCTGCGTTTAGATCTTATATCGAAAGTTAAAGGAATTGCTGCTGCGGAGAATTATTTCTGTGATCTGTCGCCGTCTGCGAAGAATCGATTTACTTATGGAGCTCTTTTGAATTGCTATTGCAAGGAATTGATGGAGGAAAAGGCATTGGCTCTTTCTAAGAAGATAGATGAGTTGAAGTTTGCTTCCAATTTGTCCTTTAACAATCTTATGACCATGTATATGAGAATGGATCAACCCGAGAAAGTACCTCCTCTTATAGATGAAATGAAGCAGAGAGGGATTTTTCTTAGCACGTACACATACAATGTTTGGATGAACAGTTGTGCTTCCCTGAATGGCGTTGGAAAAGTTGAAGAAATTCTCGAGGAGATGAAAAACGAAGACAGAAACAAATTTGATTGGACGACGTTTTCGAACTTGGCTGCTATCTATGTTAAGGCAGGACAGCTCGAGAAAGCTGAATTAGCTCTTAAGAAGGTAGAGAACGAGATCAAATCGAATAAGCAGCAGGATCGTCTAGCGTACCATTTCTTGATAAGCCTTTATGCATCGACGTCGAATCGGAGTGAGGTGTATAGGATATGGAATGCACTGAAATCAGTTTATCCAATGACAAATAACATGAGTTATCTCGTCATGCTTCAGGCTCTAAGCAAACTAAAGGATTTCGAGGGTCTTAAAAGCACTTATAAGGAATGGGAATCTAGTTGCTCGAGCTTTGATTTGCGGTTAGCGGATGTTACGATCGGGGCTTACCTACGACAGGACATGTACAAAGATGCTGCATTGGTCTTTGAGGATGCTATTAAGAGAAGTAAAGGACCTTTCTTTAGGGCTCGAGAAATGTTCATGATTTACTTCTTGAAGTTCAAGCAAGTCGATTTGGCACTCAGTCATTTGGAATCAGCTATATCGGAAAGCATGGACGATGAATGGCATCCATCACCGGCGATGGCGAATGCTTTTCTGATGTACTTTGAGGAAGAGAAAGACGTTGAAGGCGCCGAAGATTTTGCCAGGATTTTGAAGAGATTTAAGTGTCTCGACGCTAGTGCATACCATCTATTGCTCAAGACCTATGCAGCTGCAGGAAAACGAGCCCCCGACATGCGACAAAGATTGATAGAAGACAACATTGAAGTAAGTAGTGAGCTTGAGGAGTTGTTAAGTAGACAACATTGA

Protein sequence

MNRRSLLSRASAGLRHLCTSTAESKRGPVNDQQRLYPRLSKLGATGGSVAQTLNQYIMEGKIVKKYELERCIKELRKYRRYHHALQIMEWMEMRKINYSFTDYALRLDLISKVKGIAAAENYFCDLSPSAKNRFTYGALLNCYCKELMEEKALALSKKIDELKFASNLSFNNLMTMYMRMDQPEKVPPLIDEMKQRGIFLSTYTYNVWMNSCASLNGVGKVEEILEEMKNEDRNKFDWTTFSNLAAIYVKAGQLEKAELALKKVENEIKSNKQQDRLAYHFLISLYASTSNRSEVYRIWNALKSVYPMTNNMSYLVMLQALSKLKDFEGLKSTYKEWESSCSSFDLRLADVTIGAYLRQDMYKDAALVFEDAIKRSKGPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDDEWHPSPAMANAFLMYFEEEKDVEGAEDFARILKRFKCLDASAYHLLLKTYAAAGKRAPDMRQRLIEDNIEVSSELEELLSRQH
Homology
BLAST of Carg08054 vs. NCBI nr
Match: KAG7030878.1 (Pentatricopeptide repeat-containing protein, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 978.8 bits (2529), Expect = 1.8e-281
Identity = 497/497 (100.00%), Postives = 497/497 (100.00%), Query Frame = 0

Query: 1   MNRRSLLSRASAGLRHLCTSTAESKRGPVNDQQRLYPRLSKLGATGGSVAQTLNQYIMEG 60
           MNRRSLLSRASAGLRHLCTSTAESKRGPVNDQQRLYPRLSKLGATGGSVAQTLNQYIMEG
Sbjct: 1   MNRRSLLSRASAGLRHLCTSTAESKRGPVNDQQRLYPRLSKLGATGGSVAQTLNQYIMEG 60

Query: 61  KIVKKYELERCIKELRKYRRYHHALQIMEWMEMRKINYSFTDYALRLDLISKVKGIAAAE 120
           KIVKKYELERCIKELRKYRRYHHALQIMEWMEMRKINYSFTDYALRLDLISKVKGIAAAE
Sbjct: 61  KIVKKYELERCIKELRKYRRYHHALQIMEWMEMRKINYSFTDYALRLDLISKVKGIAAAE 120

Query: 121 NYFCDLSPSAKNRFTYGALLNCYCKELMEEKALALSKKIDELKFASNLSFNNLMTMYMRM 180
           NYFCDLSPSAKNRFTYGALLNCYCKELMEEKALALSKKIDELKFASNLSFNNLMTMYMRM
Sbjct: 121 NYFCDLSPSAKNRFTYGALLNCYCKELMEEKALALSKKIDELKFASNLSFNNLMTMYMRM 180

Query: 181 DQPEKVPPLIDEMKQRGIFLSTYTYNVWMNSCASLNGVGKVEEILEEMKNEDRNKFDWTT 240
           DQPEKVPPLIDEMKQRGIFLSTYTYNVWMNSCASLNGVGKVEEILEEMKNEDRNKFDWTT
Sbjct: 181 DQPEKVPPLIDEMKQRGIFLSTYTYNVWMNSCASLNGVGKVEEILEEMKNEDRNKFDWTT 240

Query: 241 FSNLAAIYVKAGQLEKAELALKKVENEIKSNKQQDRLAYHFLISLYASTSNRSEVYRIWN 300
           FSNLAAIYVKAGQLEKAELALKKVENEIKSNKQQDRLAYHFLISLYASTSNRSEVYRIWN
Sbjct: 241 FSNLAAIYVKAGQLEKAELALKKVENEIKSNKQQDRLAYHFLISLYASTSNRSEVYRIWN 300

Query: 301 ALKSVYPMTNNMSYLVMLQALSKLKDFEGLKSTYKEWESSCSSFDLRLADVTIGAYLRQD 360
           ALKSVYPMTNNMSYLVMLQALSKLKDFEGLKSTYKEWESSCSSFDLRLADVTIGAYLRQD
Sbjct: 301 ALKSVYPMTNNMSYLVMLQALSKLKDFEGLKSTYKEWESSCSSFDLRLADVTIGAYLRQD 360

Query: 361 MYKDAALVFEDAIKRSKGPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDDEWHPSP 420
           MYKDAALVFEDAIKRSKGPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDDEWHPSP
Sbjct: 361 MYKDAALVFEDAIKRSKGPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDDEWHPSP 420

Query: 421 AMANAFLMYFEEEKDVEGAEDFARILKRFKCLDASAYHLLLKTYAAAGKRAPDMRQRLIE 480
           AMANAFLMYFEEEKDVEGAEDFARILKRFKCLDASAYHLLLKTYAAAGKRAPDMRQRLIE
Sbjct: 421 AMANAFLMYFEEEKDVEGAEDFARILKRFKCLDASAYHLLLKTYAAAGKRAPDMRQRLIE 480

Query: 481 DNIEVSSELEELLSRQH 498
           DNIEVSSELEELLSRQH
Sbjct: 481 DNIEVSSELEELLSRQH 497

BLAST of Carg08054 vs. NCBI nr
Match: KAG6600219.1 (Pentatricopeptide repeat-containing protein, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 952.2 bits (2460), Expect = 1.8e-273
Identity = 482/484 (99.59%), Postives = 483/484 (99.79%), Query Frame = 0

Query: 1   MNRRSLLSRASAGLRHLCTSTAESKRGPVNDQQRLYPRLSKLGATGGSVAQTLNQYIMEG 60
           MNRR+LLSRASAGLRHLCTSTAESKRGPVNDQQRLYPRLSKLGATGGSVAQTLNQYIMEG
Sbjct: 1   MNRRNLLSRASAGLRHLCTSTAESKRGPVNDQQRLYPRLSKLGATGGSVAQTLNQYIMEG 60

Query: 61  KIVKKYELERCIKELRKYRRYHHALQIMEWMEMRKINYSFTDYALRLDLISKVKGIAAAE 120
           KIVKKYELERCIKELRKYRRYHHALQIMEWMEMRKINYSFTDYALRLDLISKVKGIAAAE
Sbjct: 61  KIVKKYELERCIKELRKYRRYHHALQIMEWMEMRKINYSFTDYALRLDLISKVKGIAAAE 120

Query: 121 NYFCDLSPSAKNRFTYGALLNCYCKELMEEKALALSKKIDELKFASNLSFNNLMTMYMRM 180
           NYFCDLS SAKNRFTYGALLNCYCKELMEEKALALSKKIDELKFASNLSFNNLMTMYMRM
Sbjct: 121 NYFCDLSSSAKNRFTYGALLNCYCKELMEEKALALSKKIDELKFASNLSFNNLMTMYMRM 180

Query: 181 DQPEKVPPLIDEMKQRGIFLSTYTYNVWMNSCASLNGVGKVEEILEEMKNEDRNKFDWTT 240
           DQPEKVPPLIDEMKQRGIFLSTYTYNVWMNSCASLNGVGKVEEILEEMKNEDRNKFDWTT
Sbjct: 181 DQPEKVPPLIDEMKQRGIFLSTYTYNVWMNSCASLNGVGKVEEILEEMKNEDRNKFDWTT 240

Query: 241 FSNLAAIYVKAGQLEKAELALKKVENEIKSNKQQDRLAYHFLISLYASTSNRSEVYRIWN 300
           FSNLAAIYVKAGQLEKAELALKKVENEIKSNKQQDRLAYHFLISLYASTSNRSEVYRIWN
Sbjct: 241 FSNLAAIYVKAGQLEKAELALKKVENEIKSNKQQDRLAYHFLISLYASTSNRSEVYRIWN 300

Query: 301 ALKSVYPMTNNMSYLVMLQALSKLKDFEGLKSTYKEWESSCSSFDLRLADVTIGAYLRQD 360
           ALKSVYPMTNNMSYLVMLQALSKLKDFEGLKSTYKEWESSCSSFDLRLADVTIGAYLRQD
Sbjct: 301 ALKSVYPMTNNMSYLVMLQALSKLKDFEGLKSTYKEWESSCSSFDLRLADVTIGAYLRQD 360

Query: 361 MYKDAALVFEDAIKRSKGPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDDEWHPSP 420
           MYKDAALVFEDAIKRSKGPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDDEWHPSP
Sbjct: 361 MYKDAALVFEDAIKRSKGPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDDEWHPSP 420

Query: 421 AMANAFLMYFEEEKDVEGAEDFARILKRFKCLDASAYHLLLKTYAAAGKRAPDMRQRLIE 480
           AMANAFLMYFEEEKDVEGAEDFARILKRFKCLDASAYHLLLKTYAAAGKRAPDMRQRLIE
Sbjct: 421 AMANAFLMYFEEEKDVEGAEDFARILKRFKCLDASAYHLLLKTYAAAGKRAPDMRQRLIE 480

Query: 481 DNIE 485
           DNIE
Sbjct: 481 DNIE 484

BLAST of Carg08054 vs. NCBI nr
Match: XP_022942939.1 (pentatricopeptide repeat-containing protein At1g02370, mitochondrial-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 949.9 bits (2454), Expect = 8.8e-273
Identity = 480/484 (99.17%), Postives = 483/484 (99.79%), Query Frame = 0

Query: 1   MNRRSLLSRASAGLRHLCTSTAESKRGPVNDQQRLYPRLSKLGATGGSVAQTLNQYIMEG 60
           MNRRSLLSRASAGLRHLCTSTAESKRGPVNDQQRLYPRLSKLGATGGSVAQTLNQYIMEG
Sbjct: 1   MNRRSLLSRASAGLRHLCTSTAESKRGPVNDQQRLYPRLSKLGATGGSVAQTLNQYIMEG 60

Query: 61  KIVKKYELERCIKELRKYRRYHHALQIMEWMEMRKINYSFTDYALRLDLISKVKGIAAAE 120
           KIVKKYELERCIKELRKYRRYHHALQIMEWMEMRKINYSFTDYALRLDLISKVKGIAAAE
Sbjct: 61  KIVKKYELERCIKELRKYRRYHHALQIMEWMEMRKINYSFTDYALRLDLISKVKGIAAAE 120

Query: 121 NYFCDLSPSAKNRFTYGALLNCYCKELMEEKALALSKKIDELKFASNLSFNNLMTMYMRM 180
           NYFCDLS SAKNRFTYGALLNCYCKELMEEKALALSKKIDELKFASNLSFNNLMTMYMRM
Sbjct: 121 NYFCDLSSSAKNRFTYGALLNCYCKELMEEKALALSKKIDELKFASNLSFNNLMTMYMRM 180

Query: 181 DQPEKVPPLIDEMKQRGIFLSTYTYNVWMNSCASLNGVGKVEEILEEMKNEDRNKFDWTT 240
           DQPEKVPPLIDEMK+RGIFLSTYTYNVWMNSCASLNGVGKVEEILEEMKNEDRNKFDWTT
Sbjct: 181 DQPEKVPPLIDEMKRRGIFLSTYTYNVWMNSCASLNGVGKVEEILEEMKNEDRNKFDWTT 240

Query: 241 FSNLAAIYVKAGQLEKAELALKKVENEIKSNKQQDRLAYHFLISLYASTSNRSEVYRIWN 300
           FSNLAAIYVKAGQLEKAELALKKVENEIKSNKQQDRLAYHFLISLYASTSNRSEVYRIWN
Sbjct: 241 FSNLAAIYVKAGQLEKAELALKKVENEIKSNKQQDRLAYHFLISLYASTSNRSEVYRIWN 300

Query: 301 ALKSVYPMTNNMSYLVMLQALSKLKDFEGLKSTYKEWESSCSSFDLRLADVTIGAYLRQD 360
           ALKSVYPMTNNMSYLVMLQALSKLKDFEGLKSTYKEWESSCSSFDLRLADVTIGAYLRQD
Sbjct: 301 ALKSVYPMTNNMSYLVMLQALSKLKDFEGLKSTYKEWESSCSSFDLRLADVTIGAYLRQD 360

Query: 361 MYKDAALVFEDAIKRSKGPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDDEWHPSP 420
           MY+DAALVFEDAIKRSKGPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDDEWHPSP
Sbjct: 361 MYEDAALVFEDAIKRSKGPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDDEWHPSP 420

Query: 421 AMANAFLMYFEEEKDVEGAEDFARILKRFKCLDASAYHLLLKTYAAAGKRAPDMRQRLIE 480
           AMANAFLMYFEEEKD+EGAEDFARILKRFKCLDASAYHLLLKTYAAAGKRAPDMRQRLIE
Sbjct: 421 AMANAFLMYFEEEKDIEGAEDFARILKRFKCLDASAYHLLLKTYAAAGKRAPDMRQRLIE 480

Query: 481 DNIE 485
           DNIE
Sbjct: 481 DNIE 484

BLAST of Carg08054 vs. NCBI nr
Match: XP_023512291.1 (pentatricopeptide repeat-containing protein At1g02370, mitochondrial-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 940.6 bits (2430), Expect = 5.3e-270
Identity = 479/485 (98.76%), Postives = 480/485 (98.97%), Query Frame = 0

Query: 1   MNRRSLLSRASAGLRHLCTSTAESKRGPVND-QQRLYPRLSKLGATGGSVAQTLNQYIME 60
           MNRRSLLSRASAGLRHLCTS AES RGPVND QQRLYPRLSKLGATGGSVAQTLNQYIME
Sbjct: 1   MNRRSLLSRASAGLRHLCTSNAESSRGPVNDQQQRLYPRLSKLGATGGSVAQTLNQYIME 60

Query: 61  GKIVKKYELERCIKELRKYRRYHHALQIMEWMEMRKINYSFTDYALRLDLISKVKGIAAA 120
           GKIVKKYELERCIKELRKYRRYHHALQIMEWMEMRKINYSFTDYALRLDLISKVKGIAAA
Sbjct: 61  GKIVKKYELERCIKELRKYRRYHHALQIMEWMEMRKINYSFTDYALRLDLISKVKGIAAA 120

Query: 121 ENYFCDLSPSAKNRFTYGALLNCYCKELMEEKALALSKKIDELKFASNLSFNNLMTMYMR 180
           ENYFCDLS SAKNRFTYGALLNCYCKELMEEKALALSKKIDELKFASNLSFNNLMTMYMR
Sbjct: 121 ENYFCDLSSSAKNRFTYGALLNCYCKELMEEKALALSKKIDELKFASNLSFNNLMTMYMR 180

Query: 181 MDQPEKVPPLIDEMKQRGIFLSTYTYNVWMNSCASLNGVGKVEEILEEMKNEDRNKFDWT 240
           MDQPEKVPPLIDEMK+RGIFLSTYTYNVWMNSCASLNGVGKVEEILEEMKNEDRNKFDWT
Sbjct: 181 MDQPEKVPPLIDEMKRRGIFLSTYTYNVWMNSCASLNGVGKVEEILEEMKNEDRNKFDWT 240

Query: 241 TFSNLAAIYVKAGQLEKAELALKKVENEIKSNKQQDRLAYHFLISLYASTSNRSEVYRIW 300
           TFSNLAAIYVKAGQLEKAELALKKVENEIKSNKQQDRLAYHFLISLYASTSNRSEVYRIW
Sbjct: 241 TFSNLAAIYVKAGQLEKAELALKKVENEIKSNKQQDRLAYHFLISLYASTSNRSEVYRIW 300

Query: 301 NALKSVYPMTNNMSYLVMLQALSKLKDFEGLKSTYKEWESSCSSFDLRLADVTIGAYLRQ 360
           NALKSVYPMTNNMSYLVMLQALSKLKDFEGLKSTYKEWESSCSSFDLRLADVTIGAYLRQ
Sbjct: 301 NALKSVYPMTNNMSYLVMLQALSKLKDFEGLKSTYKEWESSCSSFDLRLADVTIGAYLRQ 360

Query: 361 DMYKDAALVFEDAIKRSKGPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDDEWHPS 420
           DMYKDAALVFEDAIKRSKGPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDDEWHPS
Sbjct: 361 DMYKDAALVFEDAIKRSKGPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDDEWHPS 420

Query: 421 PAMANAFLMYFEEEKDVEGAEDFARILKRFKCLDASAYHLLLKTYAAAGKRAPDMRQRLI 480
           PAMANAFLMYFEEEKDVEGAEDFARILKRFKCLDASAYHLLLKTYAAAGKRAPDMRQRL 
Sbjct: 421 PAMANAFLMYFEEEKDVEGAEDFARILKRFKCLDASAYHLLLKTYAAAGKRAPDMRQRLK 480

Query: 481 EDNIE 485
           EDNIE
Sbjct: 481 EDNIE 485

BLAST of Carg08054 vs. NCBI nr
Match: XP_023005130.1 (pentatricopeptide repeat-containing protein At1g02370, mitochondrial-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 925.2 bits (2390), Expect = 2.3e-265
Identity = 471/485 (97.11%), Postives = 475/485 (97.94%), Query Frame = 0

Query: 1   MNRRSLLSRASAGLRHLCTSTAESKRGPVND-QQRLYPRLSKLGATGGSVAQTLNQYIME 60
           MNRRSLLSRASAGLRHLCTS AE  RGPVND QQRLYPRLSKLGATGGSVAQTLNQYIME
Sbjct: 1   MNRRSLLSRASAGLRHLCTSAAELTRGPVNDQQQRLYPRLSKLGATGGSVAQTLNQYIME 60

Query: 61  GKIVKKYELERCIKELRKYRRYHHALQIMEWMEMRKINYSFTDYALRLDLISKVKGIAAA 120
           GKIVKKYELERCIKELRKYRRYHHALQIMEWMEMRKINYSFTDYALRLDLISKVKGIAAA
Sbjct: 61  GKIVKKYELERCIKELRKYRRYHHALQIMEWMEMRKINYSFTDYALRLDLISKVKGIAAA 120

Query: 121 ENYFCDLSPSAKNRFTYGALLNCYCKELMEEKALALSKKIDELKFASNLSFNNLMTMYMR 180
           ENYFCDLS SAKNRFTYGALLNCYCKELMEEKALALSKKIDELKFASNLSFNNLMTMYMR
Sbjct: 121 ENYFCDLSSSAKNRFTYGALLNCYCKELMEEKALALSKKIDELKFASNLSFNNLMTMYMR 180

Query: 181 MDQPEKVPPLIDEMKQRGIFLSTYTYNVWMNSCASLNGVGKVEEILEEMKNEDRNKFDWT 240
           MDQPEKVPPLIDEMK+RGIFLSTYTYNVWMNSCASLNGVGKVEEILEEMK+EDRNKFDWT
Sbjct: 181 MDQPEKVPPLIDEMKRRGIFLSTYTYNVWMNSCASLNGVGKVEEILEEMKDEDRNKFDWT 240

Query: 241 TFSNLAAIYVKAGQLEKAELALKKVENEIKSNKQQDRLAYHFLISLYASTSNRSEVYRIW 300
           TFSNLAAIYVK GQLEKAELALKKVENEIKS++QQDRLAYHFLISLYASTSNRSEVYRIW
Sbjct: 241 TFSNLAAIYVKVGQLEKAELALKKVENEIKSSRQQDRLAYHFLISLYASTSNRSEVYRIW 300

Query: 301 NALKSVYPMTNNMSYLVMLQALSKLKDFEGLKSTYKEWESSCSSFDLRLADVTIGAYLRQ 360
           NALKSVYPMTNNMSYLVMLQALSKLKDFEGLK TYKEWESSCSSFDLRLADVTIGAYLRQ
Sbjct: 301 NALKSVYPMTNNMSYLVMLQALSKLKDFEGLKRTYKEWESSCSSFDLRLADVTIGAYLRQ 360

Query: 361 DMYKDAALVFEDAIKRSKGPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDDEWHPS 420
           DMYKDA LVFEDA KRSKGPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDDEWHPS
Sbjct: 361 DMYKDAVLVFEDANKRSKGPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDDEWHPS 420

Query: 421 PAMANAFLMYFEEEKDVEGAEDFARILKRFKCLDASAYHLLLKTYAAAGKRAPDMRQRLI 480
           PAMANAFLMYFEEEKDVEGAEDFARILKRFKCLDASAYHLLLKTYAAAGKRAPDMRQRL 
Sbjct: 421 PAMANAFLMYFEEEKDVEGAEDFARILKRFKCLDASAYHLLLKTYAAAGKRAPDMRQRLK 480

Query: 481 EDNIE 485
           EDNIE
Sbjct: 481 EDNIE 485

BLAST of Carg08054 vs. ExPASy Swiss-Prot
Match: Q9FZ24 (Pentatricopeptide repeat-containing protein At1g02370, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g02370 PE=2 SV=1)

HSP 1 Score: 489.6 bits (1259), Expect = 4.3e-137
Identity = 249/466 (53.43%), Postives = 338/466 (72.53%), Query Frame = 0

Query: 32  QQRLYPRLSKLGATGGSVAQTLNQYIMEGKIVKKYELERCIKELRKYRRYHHALQIMEWM 91
           Q+ LY +LS L  TGG+VA+TLNQ+IMEG  V+K +L RC K LRK+RR  HA +I +WM
Sbjct: 70  QRELYKKLSMLSVTGGTVAETLNQFIMEGITVRKDDLFRCAKTLRKFRRPQHAFEIFDWM 129

Query: 92  EMRKINYSFTDYALRLDLISKVKGIAAAENYFCDLSPSAKN-RFTYGALLNCYCKELMEE 151
           E RK+ +S +D+A+ LDLI K KG+ AAENYF +L PSAKN + TYGAL+NCYC EL EE
Sbjct: 130 EKRKMTFSVSDHAICLDLIGKTKGLEAAENYFNNLDPSAKNHQSTYGALMNCYCVELEEE 189

Query: 152 KALALSKKIDELKFASN-LSFNNLMTMYMRMDQPEKVPPLIDEMKQRGIFLSTYTYNVWM 211
           KA A  + +DEL F +N L FNN+M+MYMR+ QPEKVP L+D MKQRGI     TY++WM
Sbjct: 190 KAKAHFEIMDELNFVNNSLPFNNMMSMYMRLSQPEKVPVLVDAMKQRGISPCGVTYSIWM 249

Query: 212 NSCASLNGVGKVEEILEEMKNEDRNKFDWTTFSNLAAIYVKAGQLEKAELALKKVENEIK 271
            SC SLN +  +E+I++EM  +   K  W TFSNLAAIY KAG  EKA+ ALK +E ++ 
Sbjct: 250 QSCGSLNDLDGLEKIIDEMGKDSEAKTTWNTFSNLAAIYTKAGLYEKADSALKSMEEKMN 309

Query: 272 SNKQQDRLAYHFLISLYASTSNRSEVYRIWNALKSVYPMTNNMSYLVMLQALSKLKDFEG 331
            N   +R ++HFL+SLYA  S   EVYR+W +LK   P  NN+SYLVMLQA+SKL D +G
Sbjct: 310 PN---NRDSHHFLMSLYAGISKGPEVYRVWESLKKARPEVNNLSYLVMLQAMSKLGDLDG 369

Query: 332 LKSTYKEWESSCSSFDLRLADVTIGAYLRQDMYKDAALVFEDAIKRSKGPFFRAREMFMI 391
           +K  + EWES C ++D+RLA++ I  YL+ +MY++A  + + A+K+SKGPF +AR++ MI
Sbjct: 370 IKKIFTEWESKCWAYDMRLANIAINTYLKGNMYEEAEKILDGAMKKSKGPFSKARQLLMI 429

Query: 392 YFLKFKQVDLALSHLESAISESMD--DEWHPSPAMANAFLMYFEEEKDVEGAEDFARILK 451
           + L+  + DLA+ HLE+A+S+S +  DEW  S  + + F ++FE+ KDV+GAEDF +IL 
Sbjct: 430 HLLENDKADLAMKHLEAAVSDSAENKDEWGWSSELVSLFFLHFEKAKDVDGAEDFCKILS 489

Query: 452 RFKCLDASAYHLLLKTYAAAGKRAPDMRQRLIEDNIEVSSELEELL 494
            +K LD+     L+KTYAAA K +PDMR+RL +  IEVS E+++LL
Sbjct: 490 NWKPLDSETMTFLIKTYAAAEKTSPDMRERLSQQQIEVSEEIQDLL 532

BLAST of Carg08054 vs. ExPASy Swiss-Prot
Match: Q93WC5 (Pentatricopeptide repeat-containing protein At4g01990, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At4g01990 PE=2 SV=1)

HSP 1 Score: 437.6 bits (1124), Expect = 1.9e-121
Identity = 235/488 (48.16%), Postives = 324/488 (66.39%), Query Frame = 0

Query: 17  LCTSTAE-------SKRGPVNDQQRLYPRLSKLGAT-GGSVAQTLNQYIMEGKIVKKYEL 76
           L T+TAE       S        + +Y +LS LG   GG + +TLNQ++MEG  VKK++L
Sbjct: 16  LATATAEISGEAAASVPTKAKKHRSIYKKLSSLGTRGGGKMEETLNQFVMEGVPVKKHDL 75

Query: 77  ERCIKELRKYRRYHHALQIMEWMEMRKINYSFTDYALRLDLISKVKGIAAAENYFCDLSP 136
            R  K+LRK+R+   AL+I EWME ++I ++ +D+A+RL+LI+K KG+ AAE YF  L  
Sbjct: 76  IRYAKDLRKFRQPQRALEIFEWMERKEIAFTGSDHAIRLNLIAKSKGLEAAETYFNSLDD 135

Query: 137 SAKNRFTYGALLNCYCKELMEEKALALSKKIDELKFASN-LSFNNLMTMYMRMDQPEKVP 196
           S KN+ TYG+LLNCYC E  E KA A  + + +L   SN L FNNLM MYM + QPEKVP
Sbjct: 136 SIKNQSTYGSLLNCYCVEKEEVKAKAHFENMVDLNHVSNSLPFNNLMAMYMGLGQPEKVP 195

Query: 197 PLIDEMKQRGIFLSTYTYNVWMNSCASLNGVGKVEEILEEMKNEDRNKFDWTTFSNLAAI 256
            L+  MK++ I     TY++W+ SC SL  +  VE++L+EMK E    F W TF+NLAAI
Sbjct: 196 ALVVAMKEKSITPCDITYSMWIQSCGSLKDLDGVEKVLDEMKAEGEGIFSWNTFANLAAI 255

Query: 257 YVKAGQLEKAELALKKVENEIKSNKQQDRLAYHFLISLYASTSNRSEVYRIWNALKSVYP 316
           Y+K G   KAE ALK +EN +  +    R  YHFLI+LY   +N SEVYR+W+ LK  YP
Sbjct: 256 YIKVGLYGKAEEALKSLENNMNPDV---RDCYHFLINLYTGIANASEVYRVWDLLKKRYP 315

Query: 317 MTNNMSYLVMLQALSKLKDFEGLKSTYKEWESSCSSFDLRLADVTIGAYLRQDMYKDAAL 376
             NN SYL ML+ALSKL D +G+K  + EWES+C ++D+R+A+V I +YL+Q+MY++A  
Sbjct: 316 NVNNSSYLTMLRALSKLDDIDGVKKVFAEWESTCWTYDMRMANVAISSYLKQNMYEEAEA 375

Query: 377 VFEDAIKRSKGPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDDEWHPSPAMANAFL 436
           VF  A+K+ KG F +AR++ M++ LK  Q DLAL H E+A+ +  D  W  S  + ++F 
Sbjct: 376 VFNGAMKKCKGQFSKARQLLMMHLLKNDQADLALKHFEAAVLD-QDKNWTWSSELISSFF 435

Query: 437 MYFEEEKDVEGAEDFARILKRFKCLDASAYHLLLKTYAAAGKRAPDMRQRLIEDNIEVSS 496
           ++FEE KDV+GAE+F + L ++  L +  Y LL+KTY AAGK  PDM++RL E  I V  
Sbjct: 436 LHFEEAKDVDGAEEFCKTLTKWSPLSSETYTLLMKTYLAAGKACPDMKKRLEEQGILVDE 495

BLAST of Carg08054 vs. ExPASy Swiss-Prot
Match: O22714 (Pentatricopeptide repeat-containing protein At1g60770 OS=Arabidopsis thaliana OX=3702 GN=At1g60770 PE=1 SV=1)

HSP 1 Score: 366.7 bits (940), Expect = 4.2e-100
Identity = 206/485 (42.47%), Postives = 302/485 (62.27%), Query Frame = 0

Query: 14  LRHLCTSTAESKRGPVN-DQQRLYPRLSKLGATGGSVAQTLNQYIMEGKIVKKYELERCI 73
           +RHL  S   +KR      ++ LY RL K G T   V Q LNQ++   K V K+E+   I
Sbjct: 3   MRHLSRSRDVTKRSTKKYIEEPLYNRLFKDGGTEVKVRQQLNQFLKGTKHVFKWEVGDTI 62

Query: 74  KELRKYRRYHHALQIMEWMEMRKINYSFTDYALRLDLISKVKGIAAAENYFCDLSPSAKN 133
           K+LR    Y+ AL++ E ME R +N + +D A+ LDL++K + I A ENYF DL  ++K 
Sbjct: 63  KKLRNRGLYYPALKLSEVMEERGMNKTVSDQAIHLDLVAKAREITAGENYFVDLPETSKT 122

Query: 134 RFTYGALLNCYCKELMEEKALALSKKIDELKFA-SNLSFNNLMTMYMRMDQPEKVPPLID 193
             TYG+LLNCYCKEL+ EKA  L  K+ EL    S++S+N+LMT+Y +  + EKVP +I 
Sbjct: 123 ELTYGSLLNCYCKELLTEKAEGLLNKMKELNITPSSMSYNSLMTLYTKTGETEKVPAMIQ 182

Query: 194 EMKQRGIFLSTYTYNVWMNSCASLNGVGKVEEILEEMKNEDRNKFDWTTFSNLAAIYVKA 253
           E+K   +   +YTYNVWM + A+ N +  VE ++EEM  + R   DWTT+SN+A+IYV A
Sbjct: 183 ELKAENVMPDSYTYNVWMRALAATNDISGVERVIEEMNRDGRVAPDWTTYSNMASIYVDA 242

Query: 254 GQLEKAELALKKVENEIKSNKQQDRLAYHFLISLYASTSNRSEVYRIWNALKSVYPMTNN 313
           G  +KAE AL+++E     N Q+D  AY FLI+LY      +EVYRIW +L+   P T+N
Sbjct: 243 GLSQKAEKALQELE---MKNTQRDFTAYQFLITLYGRLGKLTEVYRIWRSLRLAIPKTSN 302

Query: 314 MSYLVMLQALSKLKDFEGLKSTYKEWESSCSSFDLRLADVTIGAYLRQDMYKDAALVFED 373
           ++YL M+Q L KL D  G ++ +KEW+++CS++D+R+ +V IGAY ++ + + A  + E 
Sbjct: 303 VAYLNMIQVLVKLNDLPGAETLFKEWQANCSTYDIRIVNVLIGAYAQEGLIQKANELKEK 362

Query: 374 AIKRSKGPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDD--EWHPSPAMANAFLMY 433
           A +R      +  E+FM Y++K   +  AL  +  A+S    D  +W PSP    A + Y
Sbjct: 363 APRRGGKLNAKTWEIFMDYYVKSGDMARALECMSKAVSIGKGDGGKWLPSPETVRALMSY 422

Query: 434 FEEEKDVEGAEDFARILKR-FKCLDASAYHLLLKTYAAAGKRAPDMRQRLIEDNIEVSSE 493
           FE++KDV GAE+   ILK     + A  +  L++TYAAAGK  P MR+RL  +N+EV+  
Sbjct: 423 FEQKKDVNGAENLLEILKNGTDNIGAEIFEPLIRTYAAAGKSHPAMRRRLKMENVEVNEA 482

BLAST of Carg08054 vs. ExPASy Swiss-Prot
Match: Q8LPS6 (Pentatricopeptide repeat-containing protein At1g02150 OS=Arabidopsis thaliana OX=3702 GN=At1g02150 PE=2 SV=2)

HSP 1 Score: 285.8 bits (730), Expect = 9.3e-76
Identity = 158/444 (35.59%), Postives = 256/444 (57.66%), Query Frame = 0

Query: 21  TAESKRGPVNDQQRLYPRLSKLGATGGSVAQTLNQYIMEGKIVKKYELERCIKELRKYRR 80
           T + +R P+     +Y ++S +       A  LNQ+   G+ + K+EL R +KELRKY+R
Sbjct: 55  TVDYERRPIVQWNAIYKKISLMEKPELGAASVLNQWEKAGRKLTKWELCRVVKELRKYKR 114

Query: 81  YHHALQIMEWMEMR--KINYSFTDYALRLDLISKVKGIAAAENYFCDLSPSAKNRFTYGA 140
            + AL++ +WM  R  +   S +D A++LDLI KV+GI  AE +F  L  + K+R  YG+
Sbjct: 115 ANQALEVYDWMNNRGERFRLSASDAAIQLDLIGKVRGIPDAEEFFLQLPENFKDRRVYGS 174

Query: 141 LLNCYCKELMEEKALALSKKIDELKFASN-LSFNNLMTMYMRMDQPEKVPPLIDEMKQRG 200
           LLN Y +    EKA AL   + +  +A + L FN +MT+YM + + +KV  ++ EMKQ+ 
Sbjct: 175 LLNAYVRAKSREKAEALLNTMRDKGYALHPLPFNVMMTLYMNLREYDKVDAMVFEMKQKD 234

Query: 201 IFLSTYTYNVWMNSCASLNGVGKVEEILEEMKNEDRNKFDWTTFSNLAAIYVKAGQLEKA 260
           I L  Y+YN+W++SC SL  V K+E + ++MK++     +WTTFS +A +Y+K G+ EKA
Sbjct: 235 IRLDIYSYNIWLSSCGSLGSVEKMELVYQQMKSDVSIYPNWTTFSTMATMYIKMGETEKA 294

Query: 261 ELALKKVENEIKSNKQQDRLAYHFLISLYASTSNRSEVYRIWNALKSVYPMTNNMSYLVM 320
           E AL+KVE  I     ++R+ YH+L+SLY S  N+ E+YR+W+  KSV P   N+ Y  +
Sbjct: 295 EDALRKVEARITG---RNRIPYHYLLSLYGSLGNKKELYRVWHVYKSVVPSIPNLGYHAL 354

Query: 321 LQALSKLKDFEGLKSTYKEWESSCSSFDLRLADVTIGAYLRQDMYKDAALVFEDAIKRSK 380
           + +L ++ D EG +  Y+EW    SS+D R+ ++ + AY++ D  + A  +F+  ++   
Sbjct: 355 VSSLVRMGDIEGAEKVYEEWLPVKSSYDPRIPNLLMNAYVKNDQLETAEGLFDHMVEMGG 414

Query: 381 GPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDDEWHPSPAMANAFLMYFEEEKDVE 440
            P     E+  +   + + +  AL+ L +A S      W P   M + F    EEE DV 
Sbjct: 415 KPSSSTWEILAVGHTRKRCISEALTCLRNAFSAEGSSNWRPKVLMLSGFFKLCEEESDVT 474

Query: 441 GAEDFARILKRFKCLDASAYHLLL 462
             E    +L++   L+  +Y  L+
Sbjct: 475 SKEAVLELLRQSGDLEDKSYLALI 495

BLAST of Carg08054 vs. ExPASy Swiss-Prot
Match: Q9SY07 (Pentatricopeptide repeat-containing protein At4g02820, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At4g02820 PE=2 SV=1)

HSP 1 Score: 256.1 bits (653), Expect = 7.9e-67
Identity = 163/475 (34.32%), Postives = 261/475 (54.95%), Query Frame = 0

Query: 21  TAESKRGPVNDQQRLYPRLSKLGATGGSVAQTLNQYIMEGKIVKKYELERCIKELRKYRR 80
           +A  K   V  +  L  RL  L  T  S   T+ ++  EG  V+KYEL R ++ELRK +R
Sbjct: 49  SANKKETVVGGRDTLGGRLLSLVYTKRSAVVTIRKWKEEGHSVRKYELNRIVRELRKIKR 108

Query: 81  YHHALQIMEWMEMRK-INYSFTDYALRLDLISKVKGIAAAENYFCDLSPSAKNRFTYGAL 140
           Y HAL+I EWM +++ I     DYA+ LDLISK++G+ +AE +F D+    +      +L
Sbjct: 109 YKHALEICEWMVVQEDIKLQAGDYAVHLDLISKIRGLNSAEKFFEDMPDQMRGHAACTSL 168

Query: 141 LNCYCKELMEEKALALSKKIDELKF-ASNLSFNNLMTMYMRMDQPEKVPPLIDEMKQRGI 200
           L+ Y +  + +KA AL +K+ E  F  S L +N++++MY+   Q EKVP LI E+K R  
Sbjct: 169 LHSYVQNKLSDKAEALFEKMGECGFLKSCLPYNHMLSMYISRGQFEKVPVLIKELKIR-T 228

Query: 201 FLSTYTYNVWMNSCASLNGVGKVEEILEEMKNEDRNKFDWTTFSNLAAIYVKAGQLEKAE 260
                TYN+W+ + AS N V   E++  + K E  N  DW T+S L  +Y K   +EKA 
Sbjct: 229 SPDIVTYNLWLTAFASGNDVEGAEKVYLKAKEEKLNP-DWVTYSVLTNLYAKTDNVEKAR 288

Query: 261 LALKKVENEIKSNKQQDRLAYHFLISLYASTSNRSEVYRIWNALKSVYPMTNNMSYLVML 320
           LALK++E   K   +++R+AY  LISL+A+  ++  V   W  +KS +   N+  YL M+
Sbjct: 289 LALKEME---KLVSKKNRVAYASLISLHANLGDKDGVNLTWKKVKSSFKKMNDAEYLSMI 348

Query: 321 QALSKLKDFEGLKSTYKEWESSCSSFDLRLADVTIGAYLRQDMYKDAALVFEDAIKRSKG 380
            A+ KL +FE  K  Y EWES   + D R+ ++ +  Y+ +D        +E  +++   
Sbjct: 349 SAVVKLGEFEQAKGLYDEWESVSGTGDARIPNLILAEYMNRDEVLLGEKFYERIVEKGIN 408

Query: 381 PFFRAREMFMIYFLKFKQVDLALSHLESAISESMDDEWHPSPAMANAFLMYFEEEKDVEG 440
           P +   E+    +LK K ++  L     AI      +W  +  +        EE+ +V+G
Sbjct: 409 PSYSTWEILTWAYLKRKDMEKVLDCFGKAIDSV--KKWTVNVRLVKGACKELEEQGNVKG 468

Query: 441 AEDFARILKRFKCLDASAYHLLLKTYAAAGKRAPDMRQRLIEDNIEVSSELEELL 494
           AE    +L++   ++   Y+ LL+TYA AG+ A  + +R+ +DN+E+  E +EL+
Sbjct: 469 AEKLMTLLQKAGYVNTQLYNSLLRTYAKAGEMALIVEERMAKDNVELDEETKELI 516

BLAST of Carg08054 vs. ExPASy TrEMBL
Match: A0A6J1FST4 (pentatricopeptide repeat-containing protein At1g02370, mitochondrial-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111447825 PE=4 SV=1)

HSP 1 Score: 949.9 bits (2454), Expect = 4.2e-273
Identity = 480/484 (99.17%), Postives = 483/484 (99.79%), Query Frame = 0

Query: 1   MNRRSLLSRASAGLRHLCTSTAESKRGPVNDQQRLYPRLSKLGATGGSVAQTLNQYIMEG 60
           MNRRSLLSRASAGLRHLCTSTAESKRGPVNDQQRLYPRLSKLGATGGSVAQTLNQYIMEG
Sbjct: 1   MNRRSLLSRASAGLRHLCTSTAESKRGPVNDQQRLYPRLSKLGATGGSVAQTLNQYIMEG 60

Query: 61  KIVKKYELERCIKELRKYRRYHHALQIMEWMEMRKINYSFTDYALRLDLISKVKGIAAAE 120
           KIVKKYELERCIKELRKYRRYHHALQIMEWMEMRKINYSFTDYALRLDLISKVKGIAAAE
Sbjct: 61  KIVKKYELERCIKELRKYRRYHHALQIMEWMEMRKINYSFTDYALRLDLISKVKGIAAAE 120

Query: 121 NYFCDLSPSAKNRFTYGALLNCYCKELMEEKALALSKKIDELKFASNLSFNNLMTMYMRM 180
           NYFCDLS SAKNRFTYGALLNCYCKELMEEKALALSKKIDELKFASNLSFNNLMTMYMRM
Sbjct: 121 NYFCDLSSSAKNRFTYGALLNCYCKELMEEKALALSKKIDELKFASNLSFNNLMTMYMRM 180

Query: 181 DQPEKVPPLIDEMKQRGIFLSTYTYNVWMNSCASLNGVGKVEEILEEMKNEDRNKFDWTT 240
           DQPEKVPPLIDEMK+RGIFLSTYTYNVWMNSCASLNGVGKVEEILEEMKNEDRNKFDWTT
Sbjct: 181 DQPEKVPPLIDEMKRRGIFLSTYTYNVWMNSCASLNGVGKVEEILEEMKNEDRNKFDWTT 240

Query: 241 FSNLAAIYVKAGQLEKAELALKKVENEIKSNKQQDRLAYHFLISLYASTSNRSEVYRIWN 300
           FSNLAAIYVKAGQLEKAELALKKVENEIKSNKQQDRLAYHFLISLYASTSNRSEVYRIWN
Sbjct: 241 FSNLAAIYVKAGQLEKAELALKKVENEIKSNKQQDRLAYHFLISLYASTSNRSEVYRIWN 300

Query: 301 ALKSVYPMTNNMSYLVMLQALSKLKDFEGLKSTYKEWESSCSSFDLRLADVTIGAYLRQD 360
           ALKSVYPMTNNMSYLVMLQALSKLKDFEGLKSTYKEWESSCSSFDLRLADVTIGAYLRQD
Sbjct: 301 ALKSVYPMTNNMSYLVMLQALSKLKDFEGLKSTYKEWESSCSSFDLRLADVTIGAYLRQD 360

Query: 361 MYKDAALVFEDAIKRSKGPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDDEWHPSP 420
           MY+DAALVFEDAIKRSKGPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDDEWHPSP
Sbjct: 361 MYEDAALVFEDAIKRSKGPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDDEWHPSP 420

Query: 421 AMANAFLMYFEEEKDVEGAEDFARILKRFKCLDASAYHLLLKTYAAAGKRAPDMRQRLIE 480
           AMANAFLMYFEEEKD+EGAEDFARILKRFKCLDASAYHLLLKTYAAAGKRAPDMRQRLIE
Sbjct: 421 AMANAFLMYFEEEKDIEGAEDFARILKRFKCLDASAYHLLLKTYAAAGKRAPDMRQRLIE 480

Query: 481 DNIE 485
           DNIE
Sbjct: 481 DNIE 484

BLAST of Carg08054 vs. ExPASy TrEMBL
Match: A0A6J1KWK9 (pentatricopeptide repeat-containing protein At1g02370, mitochondrial-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111498213 PE=4 SV=1)

HSP 1 Score: 925.2 bits (2390), Expect = 1.1e-265
Identity = 471/485 (97.11%), Postives = 475/485 (97.94%), Query Frame = 0

Query: 1   MNRRSLLSRASAGLRHLCTSTAESKRGPVND-QQRLYPRLSKLGATGGSVAQTLNQYIME 60
           MNRRSLLSRASAGLRHLCTS AE  RGPVND QQRLYPRLSKLGATGGSVAQTLNQYIME
Sbjct: 1   MNRRSLLSRASAGLRHLCTSAAELTRGPVNDQQQRLYPRLSKLGATGGSVAQTLNQYIME 60

Query: 61  GKIVKKYELERCIKELRKYRRYHHALQIMEWMEMRKINYSFTDYALRLDLISKVKGIAAA 120
           GKIVKKYELERCIKELRKYRRYHHALQIMEWMEMRKINYSFTDYALRLDLISKVKGIAAA
Sbjct: 61  GKIVKKYELERCIKELRKYRRYHHALQIMEWMEMRKINYSFTDYALRLDLISKVKGIAAA 120

Query: 121 ENYFCDLSPSAKNRFTYGALLNCYCKELMEEKALALSKKIDELKFASNLSFNNLMTMYMR 180
           ENYFCDLS SAKNRFTYGALLNCYCKELMEEKALALSKKIDELKFASNLSFNNLMTMYMR
Sbjct: 121 ENYFCDLSSSAKNRFTYGALLNCYCKELMEEKALALSKKIDELKFASNLSFNNLMTMYMR 180

Query: 181 MDQPEKVPPLIDEMKQRGIFLSTYTYNVWMNSCASLNGVGKVEEILEEMKNEDRNKFDWT 240
           MDQPEKVPPLIDEMK+RGIFLSTYTYNVWMNSCASLNGVGKVEEILEEMK+EDRNKFDWT
Sbjct: 181 MDQPEKVPPLIDEMKRRGIFLSTYTYNVWMNSCASLNGVGKVEEILEEMKDEDRNKFDWT 240

Query: 241 TFSNLAAIYVKAGQLEKAELALKKVENEIKSNKQQDRLAYHFLISLYASTSNRSEVYRIW 300
           TFSNLAAIYVK GQLEKAELALKKVENEIKS++QQDRLAYHFLISLYASTSNRSEVYRIW
Sbjct: 241 TFSNLAAIYVKVGQLEKAELALKKVENEIKSSRQQDRLAYHFLISLYASTSNRSEVYRIW 300

Query: 301 NALKSVYPMTNNMSYLVMLQALSKLKDFEGLKSTYKEWESSCSSFDLRLADVTIGAYLRQ 360
           NALKSVYPMTNNMSYLVMLQALSKLKDFEGLK TYKEWESSCSSFDLRLADVTIGAYLRQ
Sbjct: 301 NALKSVYPMTNNMSYLVMLQALSKLKDFEGLKRTYKEWESSCSSFDLRLADVTIGAYLRQ 360

Query: 361 DMYKDAALVFEDAIKRSKGPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDDEWHPS 420
           DMYKDA LVFEDA KRSKGPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDDEWHPS
Sbjct: 361 DMYKDAVLVFEDANKRSKGPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDDEWHPS 420

Query: 421 PAMANAFLMYFEEEKDVEGAEDFARILKRFKCLDASAYHLLLKTYAAAGKRAPDMRQRLI 480
           PAMANAFLMYFEEEKDVEGAEDFARILKRFKCLDASAYHLLLKTYAAAGKRAPDMRQRL 
Sbjct: 421 PAMANAFLMYFEEEKDVEGAEDFARILKRFKCLDASAYHLLLKTYAAAGKRAPDMRQRLK 480

Query: 481 EDNIE 485
           EDNIE
Sbjct: 481 EDNIE 485

BLAST of Carg08054 vs. ExPASy TrEMBL
Match: A0A6J1CK87 (pentatricopeptide repeat-containing protein At1g02370, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111011748 PE=4 SV=1)

HSP 1 Score: 851.3 bits (2198), Expect = 2.1e-243
Identity = 435/493 (88.24%), Postives = 458/493 (92.90%), Query Frame = 0

Query: 1   MNRRSLLSRASAGLRHLCTSTAESKRGPVNDQQRLYPRLSKLGATGGSVAQTLNQYIMEG 60
           MNRRSL+S   AGLR LCTS AE  RGP+NDQ+RLYPRLS LGA+GGSVA+TLNQ+IMEG
Sbjct: 1   MNRRSLIS--PAGLRQLCTSAAELTRGPLNDQRRLYPRLSALGASGGSVARTLNQFIMEG 60

Query: 61  KIVKKYELERCIKELRKYRRYHHALQIMEWMEMRKINYSFTDYALRLDLISKVKGIAAAE 120
           KIVKKYELERCIKELRKYRRYHHALQIMEWMEMR IN SFTDYALRLDLISKVKGIAAAE
Sbjct: 61  KIVKKYELERCIKELRKYRRYHHALQIMEWMEMRNINSSFTDYALRLDLISKVKGIAAAE 120

Query: 121 NYFCDLSPSAKNRFTYGALLNCYCKELMEEKALALSKKIDELKFASNLSFNNLMTMYMRM 180
           +YFCDLSPSAKNRFTYGALLNCYCKE+M+EKALALSKKIDELKFASNLSFNNLMTMYMRM
Sbjct: 121 SYFCDLSPSAKNRFTYGALLNCYCKEMMQEKALALSKKIDELKFASNLSFNNLMTMYMRM 180

Query: 181 DQPEKVPPLIDEMKQRGIFLSTYTYNVWMNSCASLNGVGKVEEILEEMKNEDRNKFDWTT 240
           +QPEKVPPLIDEMKQRGIFLSTYTYNVWMNSCASL+ VGKVEEILEEMKNEDRNKFDWTT
Sbjct: 181 NQPEKVPPLIDEMKQRGIFLSTYTYNVWMNSCASLDDVGKVEEILEEMKNEDRNKFDWTT 240

Query: 241 FSNLAAIYVKAGQLEKAELALKKVENEIKSNKQQDRLAYHFLISLYASTSNRSEVYRIWN 300
           FSNLAAIYVK GQL+KAELALKKVE EIKSNKQ+  LAYHFLISLYASTSN SEV RIW 
Sbjct: 241 FSNLAAIYVKVGQLDKAELALKKVEEEIKSNKQK-CLAYHFLISLYASTSNLSEVNRIWK 300

Query: 301 ALKSVYPMTNNMSYLVMLQALSKLKDFEGLKSTYKEWESSCSSFDLRLADVTIGAYLRQD 360
           ALKSVYPMTNN+SYLV+LQALSKLKDF+GLK TY EWESSCS FD RLA VTIGAYLRQD
Sbjct: 301 ALKSVYPMTNNISYLVILQALSKLKDFDGLKKTYNEWESSCSIFDFRLAAVTIGAYLRQD 360

Query: 361 MYKDAALVFEDAIKRSKGPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDDEWHPSP 420
           MYKDAAL+FEDAIKRSKGPF RAREMFMIYFLK KQVD ALSHLESAISES D+EWHPSP
Sbjct: 361 MYKDAALIFEDAIKRSKGPFLRAREMFMIYFLKLKQVDSALSHLESAISESKDNEWHPSP 420

Query: 421 AMANAFLMYFEEEKDVEGAEDFARILKRFKCLDASAYHLLLKTYAAAGKRAPDMRQRLIE 480
           AM NAFL YFEEEKDV+GAEDFA+ILK F CLD+SAYHLLLKTY AAGK +PDMRQRL E
Sbjct: 421 AMVNAFLKYFEEEKDVKGAEDFAKILKSFNCLDSSAYHLLLKTYVAAGKSSPDMRQRLKE 480

Query: 481 DNIEVSSELEELL 494
           D IE+SSELEELL
Sbjct: 481 DKIEISSELEELL 490

BLAST of Carg08054 vs. ExPASy TrEMBL
Match: A0A6J1FX64 (pentatricopeptide repeat-containing protein At1g02370, mitochondrial-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111447825 PE=4 SV=1)

HSP 1 Score: 782.3 bits (2019), Expect = 1.2e-222
Identity = 393/397 (98.99%), Postives = 396/397 (99.75%), Query Frame = 0

Query: 88  MEWMEMRKINYSFTDYALRLDLISKVKGIAAAENYFCDLSPSAKNRFTYGALLNCYCKEL 147
           MEWMEMRKINYSFTDYALRLDLISKVKGIAAAENYFCDLS SAKNRFTYGALLNCYCKEL
Sbjct: 1   MEWMEMRKINYSFTDYALRLDLISKVKGIAAAENYFCDLSSSAKNRFTYGALLNCYCKEL 60

Query: 148 MEEKALALSKKIDELKFASNLSFNNLMTMYMRMDQPEKVPPLIDEMKQRGIFLSTYTYNV 207
           MEEKALALSKKIDELKFASNLSFNNLMTMYMRMDQPEKVPPLIDEMK+RGIFLSTYTYNV
Sbjct: 61  MEEKALALSKKIDELKFASNLSFNNLMTMYMRMDQPEKVPPLIDEMKRRGIFLSTYTYNV 120

Query: 208 WMNSCASLNGVGKVEEILEEMKNEDRNKFDWTTFSNLAAIYVKAGQLEKAELALKKVENE 267
           WMNSCASLNGVGKVEEILEEMKNEDRNKFDWTTFSNLAAIYVKAGQLEKAELALKKVENE
Sbjct: 121 WMNSCASLNGVGKVEEILEEMKNEDRNKFDWTTFSNLAAIYVKAGQLEKAELALKKVENE 180

Query: 268 IKSNKQQDRLAYHFLISLYASTSNRSEVYRIWNALKSVYPMTNNMSYLVMLQALSKLKDF 327
           IKSNKQQDRLAYHFLISLYASTSNRSEVYRIWNALKSVYPMTNNMSYLVMLQALSKLKDF
Sbjct: 181 IKSNKQQDRLAYHFLISLYASTSNRSEVYRIWNALKSVYPMTNNMSYLVMLQALSKLKDF 240

Query: 328 EGLKSTYKEWESSCSSFDLRLADVTIGAYLRQDMYKDAALVFEDAIKRSKGPFFRAREMF 387
           EGLKSTYKEWESSCSSFDLRLADVTIGAYLRQDMY+DAALVFEDAIKRSKGPFFRAREMF
Sbjct: 241 EGLKSTYKEWESSCSSFDLRLADVTIGAYLRQDMYEDAALVFEDAIKRSKGPFFRAREMF 300

Query: 388 MIYFLKFKQVDLALSHLESAISESMDDEWHPSPAMANAFLMYFEEEKDVEGAEDFARILK 447
           MIYFLKFKQVDLALSHLESAISESMDDEWHPSPAMANAFLMYFEEEKD+EGAEDFARILK
Sbjct: 301 MIYFLKFKQVDLALSHLESAISESMDDEWHPSPAMANAFLMYFEEEKDIEGAEDFARILK 360

Query: 448 RFKCLDASAYHLLLKTYAAAGKRAPDMRQRLIEDNIE 485
           RFKCLDASAYHLLLKTYAAAGKRAPDMRQRLIEDNIE
Sbjct: 361 RFKCLDASAYHLLLKTYAAAGKRAPDMRQRLIEDNIE 397

BLAST of Carg08054 vs. ExPASy TrEMBL
Match: A0A1S3BYD8 (pentatricopeptide repeat-containing protein At1g02370, mitochondrial-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103494433 PE=4 SV=1)

HSP 1 Score: 780.8 bits (2015), Expect = 3.4e-222
Identity = 399/493 (80.93%), Postives = 429/493 (87.02%), Query Frame = 0

Query: 1   MNRRSLLSRASAGLRHLCTSTAESKRGPVNDQQRLYPRLSKLGATGGSVAQTLNQYIMEG 60
           MNRRSL+SRA AGLR LCTS AE  R P N+ + LYPRLS LGATGGSVAQT+N++IMEG
Sbjct: 1   MNRRSLISRAPAGLRQLCTSVAELTRSPANNHRGLYPRLSVLGATGGSVAQTINRFIMEG 60

Query: 61  KIVKKYELERCIKELRKYRRYHHALQIMEWMEMRKINYSFTDYALRLDLISKVKGIAAAE 120
            IVKKYELE+CIKELRKYRRY H+LQIMEWME+RKINYSFTDYALRLDLISKV GI AAE
Sbjct: 61  NIVKKYELEKCIKELRKYRRYDHSLQIMEWMEIRKINYSFTDYALRLDLISKVNGITAAE 120

Query: 121 NYFCDLSPSAKNRFTYGALLNCYCKELMEEKALALSKKIDELKFASNLSFNNLMTMYMRM 180
            YF DL PSAKNR TYGALLNCYCKE+MEEKA  L KK+DELKF ++L+FNNLMTMYMRM
Sbjct: 121 KYFYDLPPSAKNRCTYGALLNCYCKEMMEEKASTLFKKMDELKFVTSLAFNNLMTMYMRM 180

Query: 181 DQPEKVPPLIDEMKQRGIFLSTYTYNVWMNSCASLNGVGKVEEILEEMKNEDRNKFDWTT 240
           DQPEKVPPLI EMKQRG +L+T+TYNVWMNSCASLN +GKVEEILEEMK ED NK DWTT
Sbjct: 181 DQPEKVPPLIGEMKQRGFYLTTFTYNVWMNSCASLNDIGKVEEILEEMKMEDSNKLDWTT 240

Query: 241 FSNLAAIYVKAGQLEKAELALKKVENEIKSNKQQDRLAYHFLISLYASTSNRSEVYRIWN 300
           FSNLA+ YVKAGQLEKAELALKKVE EIKS+K +DRLAYH LISLYASTSN SEV RIWN
Sbjct: 241 FSNLASFYVKAGQLEKAELALKKVEEEIKSDK-KDRLAYHCLISLYASTSNLSEVNRIWN 300

Query: 301 ALKSVYPMTNNMSYLVMLQALSKLKDFEGLKSTYKEWESSCSSFDLRLADVTIGAYLRQD 360
            LKSVYP   N SYLVMLQALSKLKD EGLK TYKEWES C  FDLRL +V IGAYL+QD
Sbjct: 301 LLKSVYPTMTNTSYLVMLQALSKLKDIEGLKKTYKEWESICHIFDLRLVNVIIGAYLQQD 360

Query: 361 MYKDAALVFEDAIKRSKGPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDDEWHPSP 420
           MY+DAA++FEDAIKRSKGPF RARE FM+YFLK KQVD A SHLESAISES + EWHPS 
Sbjct: 361 MYEDAAMIFEDAIKRSKGPFSRAREKFMVYFLKLKQVDSAFSHLESAISESKEKEWHPSL 420

Query: 421 AMANAFLMYFEEEKDVEGAEDFARILKRFKCLDASAYHLLLKTYAAAGKRAPDMRQRLIE 480
           A  NAFL YFEEEKDVEGAEDFARILKR KCLD S YHLLLKTY AAGK APDMRQRL E
Sbjct: 421 ATTNAFLNYFEEEKDVEGAEDFARILKRLKCLDESGYHLLLKTYVAAGKSAPDMRQRLKE 480

Query: 481 DNIEVSSELEELL 494
           D+I +SSELEELL
Sbjct: 481 DDIGISSELEELL 492

BLAST of Carg08054 vs. TAIR 10
Match: AT1G02370.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 489.6 bits (1259), Expect = 3.0e-138
Identity = 249/466 (53.43%), Postives = 338/466 (72.53%), Query Frame = 0

Query: 32  QQRLYPRLSKLGATGGSVAQTLNQYIMEGKIVKKYELERCIKELRKYRRYHHALQIMEWM 91
           Q+ LY +LS L  TGG+VA+TLNQ+IMEG  V+K +L RC K LRK+RR  HA +I +WM
Sbjct: 70  QRELYKKLSMLSVTGGTVAETLNQFIMEGITVRKDDLFRCAKTLRKFRRPQHAFEIFDWM 129

Query: 92  EMRKINYSFTDYALRLDLISKVKGIAAAENYFCDLSPSAKN-RFTYGALLNCYCKELMEE 151
           E RK+ +S +D+A+ LDLI K KG+ AAENYF +L PSAKN + TYGAL+NCYC EL EE
Sbjct: 130 EKRKMTFSVSDHAICLDLIGKTKGLEAAENYFNNLDPSAKNHQSTYGALMNCYCVELEEE 189

Query: 152 KALALSKKIDELKFASN-LSFNNLMTMYMRMDQPEKVPPLIDEMKQRGIFLSTYTYNVWM 211
           KA A  + +DEL F +N L FNN+M+MYMR+ QPEKVP L+D MKQRGI     TY++WM
Sbjct: 190 KAKAHFEIMDELNFVNNSLPFNNMMSMYMRLSQPEKVPVLVDAMKQRGISPCGVTYSIWM 249

Query: 212 NSCASLNGVGKVEEILEEMKNEDRNKFDWTTFSNLAAIYVKAGQLEKAELALKKVENEIK 271
            SC SLN +  +E+I++EM  +   K  W TFSNLAAIY KAG  EKA+ ALK +E ++ 
Sbjct: 250 QSCGSLNDLDGLEKIIDEMGKDSEAKTTWNTFSNLAAIYTKAGLYEKADSALKSMEEKMN 309

Query: 272 SNKQQDRLAYHFLISLYASTSNRSEVYRIWNALKSVYPMTNNMSYLVMLQALSKLKDFEG 331
            N   +R ++HFL+SLYA  S   EVYR+W +LK   P  NN+SYLVMLQA+SKL D +G
Sbjct: 310 PN---NRDSHHFLMSLYAGISKGPEVYRVWESLKKARPEVNNLSYLVMLQAMSKLGDLDG 369

Query: 332 LKSTYKEWESSCSSFDLRLADVTIGAYLRQDMYKDAALVFEDAIKRSKGPFFRAREMFMI 391
           +K  + EWES C ++D+RLA++ I  YL+ +MY++A  + + A+K+SKGPF +AR++ MI
Sbjct: 370 IKKIFTEWESKCWAYDMRLANIAINTYLKGNMYEEAEKILDGAMKKSKGPFSKARQLLMI 429

Query: 392 YFLKFKQVDLALSHLESAISESMD--DEWHPSPAMANAFLMYFEEEKDVEGAEDFARILK 451
           + L+  + DLA+ HLE+A+S+S +  DEW  S  + + F ++FE+ KDV+GAEDF +IL 
Sbjct: 430 HLLENDKADLAMKHLEAAVSDSAENKDEWGWSSELVSLFFLHFEKAKDVDGAEDFCKILS 489

Query: 452 RFKCLDASAYHLLLKTYAAAGKRAPDMRQRLIEDNIEVSSELEELL 494
            +K LD+     L+KTYAAA K +PDMR+RL +  IEVS E+++LL
Sbjct: 490 NWKPLDSETMTFLIKTYAAAEKTSPDMRERLSQQQIEVSEEIQDLL 532

BLAST of Carg08054 vs. TAIR 10
Match: AT4G01990.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 437.6 bits (1124), Expect = 1.4e-122
Identity = 235/488 (48.16%), Postives = 324/488 (66.39%), Query Frame = 0

Query: 17  LCTSTAE-------SKRGPVNDQQRLYPRLSKLGAT-GGSVAQTLNQYIMEGKIVKKYEL 76
           L T+TAE       S        + +Y +LS LG   GG + +TLNQ++MEG  VKK++L
Sbjct: 16  LATATAEISGEAAASVPTKAKKHRSIYKKLSSLGTRGGGKMEETLNQFVMEGVPVKKHDL 75

Query: 77  ERCIKELRKYRRYHHALQIMEWMEMRKINYSFTDYALRLDLISKVKGIAAAENYFCDLSP 136
            R  K+LRK+R+   AL+I EWME ++I ++ +D+A+RL+LI+K KG+ AAE YF  L  
Sbjct: 76  IRYAKDLRKFRQPQRALEIFEWMERKEIAFTGSDHAIRLNLIAKSKGLEAAETYFNSLDD 135

Query: 137 SAKNRFTYGALLNCYCKELMEEKALALSKKIDELKFASN-LSFNNLMTMYMRMDQPEKVP 196
           S KN+ TYG+LLNCYC E  E KA A  + + +L   SN L FNNLM MYM + QPEKVP
Sbjct: 136 SIKNQSTYGSLLNCYCVEKEEVKAKAHFENMVDLNHVSNSLPFNNLMAMYMGLGQPEKVP 195

Query: 197 PLIDEMKQRGIFLSTYTYNVWMNSCASLNGVGKVEEILEEMKNEDRNKFDWTTFSNLAAI 256
            L+  MK++ I     TY++W+ SC SL  +  VE++L+EMK E    F W TF+NLAAI
Sbjct: 196 ALVVAMKEKSITPCDITYSMWIQSCGSLKDLDGVEKVLDEMKAEGEGIFSWNTFANLAAI 255

Query: 257 YVKAGQLEKAELALKKVENEIKSNKQQDRLAYHFLISLYASTSNRSEVYRIWNALKSVYP 316
           Y+K G   KAE ALK +EN +  +    R  YHFLI+LY   +N SEVYR+W+ LK  YP
Sbjct: 256 YIKVGLYGKAEEALKSLENNMNPDV---RDCYHFLINLYTGIANASEVYRVWDLLKKRYP 315

Query: 317 MTNNMSYLVMLQALSKLKDFEGLKSTYKEWESSCSSFDLRLADVTIGAYLRQDMYKDAAL 376
             NN SYL ML+ALSKL D +G+K  + EWES+C ++D+R+A+V I +YL+Q+MY++A  
Sbjct: 316 NVNNSSYLTMLRALSKLDDIDGVKKVFAEWESTCWTYDMRMANVAISSYLKQNMYEEAEA 375

Query: 377 VFEDAIKRSKGPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDDEWHPSPAMANAFL 436
           VF  A+K+ KG F +AR++ M++ LK  Q DLAL H E+A+ +  D  W  S  + ++F 
Sbjct: 376 VFNGAMKKCKGQFSKARQLLMMHLLKNDQADLALKHFEAAVLD-QDKNWTWSSELISSFF 435

Query: 437 MYFEEEKDVEGAEDFARILKRFKCLDASAYHLLLKTYAAAGKRAPDMRQRLIEDNIEVSS 496
           ++FEE KDV+GAE+F + L ++  L +  Y LL+KTY AAGK  PDM++RL E  I V  
Sbjct: 436 LHFEEAKDVDGAEEFCKTLTKWSPLSSETYTLLMKTYLAAGKACPDMKKRLEEQGILVDE 495

BLAST of Carg08054 vs. TAIR 10
Match: AT1G60770.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 366.7 bits (940), Expect = 3.0e-101
Identity = 206/485 (42.47%), Postives = 302/485 (62.27%), Query Frame = 0

Query: 14  LRHLCTSTAESKRGPVN-DQQRLYPRLSKLGATGGSVAQTLNQYIMEGKIVKKYELERCI 73
           +RHL  S   +KR      ++ LY RL K G T   V Q LNQ++   K V K+E+   I
Sbjct: 3   MRHLSRSRDVTKRSTKKYIEEPLYNRLFKDGGTEVKVRQQLNQFLKGTKHVFKWEVGDTI 62

Query: 74  KELRKYRRYHHALQIMEWMEMRKINYSFTDYALRLDLISKVKGIAAAENYFCDLSPSAKN 133
           K+LR    Y+ AL++ E ME R +N + +D A+ LDL++K + I A ENYF DL  ++K 
Sbjct: 63  KKLRNRGLYYPALKLSEVMEERGMNKTVSDQAIHLDLVAKAREITAGENYFVDLPETSKT 122

Query: 134 RFTYGALLNCYCKELMEEKALALSKKIDELKFA-SNLSFNNLMTMYMRMDQPEKVPPLID 193
             TYG+LLNCYCKEL+ EKA  L  K+ EL    S++S+N+LMT+Y +  + EKVP +I 
Sbjct: 123 ELTYGSLLNCYCKELLTEKAEGLLNKMKELNITPSSMSYNSLMTLYTKTGETEKVPAMIQ 182

Query: 194 EMKQRGIFLSTYTYNVWMNSCASLNGVGKVEEILEEMKNEDRNKFDWTTFSNLAAIYVKA 253
           E+K   +   +YTYNVWM + A+ N +  VE ++EEM  + R   DWTT+SN+A+IYV A
Sbjct: 183 ELKAENVMPDSYTYNVWMRALAATNDISGVERVIEEMNRDGRVAPDWTTYSNMASIYVDA 242

Query: 254 GQLEKAELALKKVENEIKSNKQQDRLAYHFLISLYASTSNRSEVYRIWNALKSVYPMTNN 313
           G  +KAE AL+++E     N Q+D  AY FLI+LY      +EVYRIW +L+   P T+N
Sbjct: 243 GLSQKAEKALQELE---MKNTQRDFTAYQFLITLYGRLGKLTEVYRIWRSLRLAIPKTSN 302

Query: 314 MSYLVMLQALSKLKDFEGLKSTYKEWESSCSSFDLRLADVTIGAYLRQDMYKDAALVFED 373
           ++YL M+Q L KL D  G ++ +KEW+++CS++D+R+ +V IGAY ++ + + A  + E 
Sbjct: 303 VAYLNMIQVLVKLNDLPGAETLFKEWQANCSTYDIRIVNVLIGAYAQEGLIQKANELKEK 362

Query: 374 AIKRSKGPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDD--EWHPSPAMANAFLMY 433
           A +R      +  E+FM Y++K   +  AL  +  A+S    D  +W PSP    A + Y
Sbjct: 363 APRRGGKLNAKTWEIFMDYYVKSGDMARALECMSKAVSIGKGDGGKWLPSPETVRALMSY 422

Query: 434 FEEEKDVEGAEDFARILKR-FKCLDASAYHLLLKTYAAAGKRAPDMRQRLIEDNIEVSSE 493
           FE++KDV GAE+   ILK     + A  +  L++TYAAAGK  P MR+RL  +N+EV+  
Sbjct: 423 FEQKKDVNGAENLLEILKNGTDNIGAEIFEPLIRTYAAAGKSHPAMRRRLKMENVEVNEA 482

BLAST of Carg08054 vs. TAIR 10
Match: AT1G02150.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 285.8 bits (730), Expect = 6.6e-77
Identity = 158/444 (35.59%), Postives = 256/444 (57.66%), Query Frame = 0

Query: 21  TAESKRGPVNDQQRLYPRLSKLGATGGSVAQTLNQYIMEGKIVKKYELERCIKELRKYRR 80
           T + +R P+     +Y ++S +       A  LNQ+   G+ + K+EL R +KELRKY+R
Sbjct: 55  TVDYERRPIVQWNAIYKKISLMEKPELGAASVLNQWEKAGRKLTKWELCRVVKELRKYKR 114

Query: 81  YHHALQIMEWMEMR--KINYSFTDYALRLDLISKVKGIAAAENYFCDLSPSAKNRFTYGA 140
            + AL++ +WM  R  +   S +D A++LDLI KV+GI  AE +F  L  + K+R  YG+
Sbjct: 115 ANQALEVYDWMNNRGERFRLSASDAAIQLDLIGKVRGIPDAEEFFLQLPENFKDRRVYGS 174

Query: 141 LLNCYCKELMEEKALALSKKIDELKFASN-LSFNNLMTMYMRMDQPEKVPPLIDEMKQRG 200
           LLN Y +    EKA AL   + +  +A + L FN +MT+YM + + +KV  ++ EMKQ+ 
Sbjct: 175 LLNAYVRAKSREKAEALLNTMRDKGYALHPLPFNVMMTLYMNLREYDKVDAMVFEMKQKD 234

Query: 201 IFLSTYTYNVWMNSCASLNGVGKVEEILEEMKNEDRNKFDWTTFSNLAAIYVKAGQLEKA 260
           I L  Y+YN+W++SC SL  V K+E + ++MK++     +WTTFS +A +Y+K G+ EKA
Sbjct: 235 IRLDIYSYNIWLSSCGSLGSVEKMELVYQQMKSDVSIYPNWTTFSTMATMYIKMGETEKA 294

Query: 261 ELALKKVENEIKSNKQQDRLAYHFLISLYASTSNRSEVYRIWNALKSVYPMTNNMSYLVM 320
           E AL+KVE  I     ++R+ YH+L+SLY S  N+ E+YR+W+  KSV P   N+ Y  +
Sbjct: 295 EDALRKVEARITG---RNRIPYHYLLSLYGSLGNKKELYRVWHVYKSVVPSIPNLGYHAL 354

Query: 321 LQALSKLKDFEGLKSTYKEWESSCSSFDLRLADVTIGAYLRQDMYKDAALVFEDAIKRSK 380
           + +L ++ D EG +  Y+EW    SS+D R+ ++ + AY++ D  + A  +F+  ++   
Sbjct: 355 VSSLVRMGDIEGAEKVYEEWLPVKSSYDPRIPNLLMNAYVKNDQLETAEGLFDHMVEMGG 414

Query: 381 GPFFRAREMFMIYFLKFKQVDLALSHLESAISESMDDEWHPSPAMANAFLMYFEEEKDVE 440
            P     E+  +   + + +  AL+ L +A S      W P   M + F    EEE DV 
Sbjct: 415 KPSSSTWEILAVGHTRKRCISEALTCLRNAFSAEGSSNWRPKVLMLSGFFKLCEEESDVT 474

Query: 441 GAEDFARILKRFKCLDASAYHLLL 462
             E    +L++   L+  +Y  L+
Sbjct: 475 SKEAVLELLRQSGDLEDKSYLALI 495

BLAST of Carg08054 vs. TAIR 10
Match: AT4G02820.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 256.1 bits (653), Expect = 5.6e-68
Identity = 163/475 (34.32%), Postives = 261/475 (54.95%), Query Frame = 0

Query: 21  TAESKRGPVNDQQRLYPRLSKLGATGGSVAQTLNQYIMEGKIVKKYELERCIKELRKYRR 80
           +A  K   V  +  L  RL  L  T  S   T+ ++  EG  V+KYEL R ++ELRK +R
Sbjct: 49  SANKKETVVGGRDTLGGRLLSLVYTKRSAVVTIRKWKEEGHSVRKYELNRIVRELRKIKR 108

Query: 81  YHHALQIMEWMEMRK-INYSFTDYALRLDLISKVKGIAAAENYFCDLSPSAKNRFTYGAL 140
           Y HAL+I EWM +++ I     DYA+ LDLISK++G+ +AE +F D+    +      +L
Sbjct: 109 YKHALEICEWMVVQEDIKLQAGDYAVHLDLISKIRGLNSAEKFFEDMPDQMRGHAACTSL 168

Query: 141 LNCYCKELMEEKALALSKKIDELKF-ASNLSFNNLMTMYMRMDQPEKVPPLIDEMKQRGI 200
           L+ Y +  + +KA AL +K+ E  F  S L +N++++MY+   Q EKVP LI E+K R  
Sbjct: 169 LHSYVQNKLSDKAEALFEKMGECGFLKSCLPYNHMLSMYISRGQFEKVPVLIKELKIR-T 228

Query: 201 FLSTYTYNVWMNSCASLNGVGKVEEILEEMKNEDRNKFDWTTFSNLAAIYVKAGQLEKAE 260
                TYN+W+ + AS N V   E++  + K E  N  DW T+S L  +Y K   +EKA 
Sbjct: 229 SPDIVTYNLWLTAFASGNDVEGAEKVYLKAKEEKLNP-DWVTYSVLTNLYAKTDNVEKAR 288

Query: 261 LALKKVENEIKSNKQQDRLAYHFLISLYASTSNRSEVYRIWNALKSVYPMTNNMSYLVML 320
           LALK++E   K   +++R+AY  LISL+A+  ++  V   W  +KS +   N+  YL M+
Sbjct: 289 LALKEME---KLVSKKNRVAYASLISLHANLGDKDGVNLTWKKVKSSFKKMNDAEYLSMI 348

Query: 321 QALSKLKDFEGLKSTYKEWESSCSSFDLRLADVTIGAYLRQDMYKDAALVFEDAIKRSKG 380
            A+ KL +FE  K  Y EWES   + D R+ ++ +  Y+ +D        +E  +++   
Sbjct: 349 SAVVKLGEFEQAKGLYDEWESVSGTGDARIPNLILAEYMNRDEVLLGEKFYERIVEKGIN 408

Query: 381 PFFRAREMFMIYFLKFKQVDLALSHLESAISESMDDEWHPSPAMANAFLMYFEEEKDVEG 440
           P +   E+    +LK K ++  L     AI      +W  +  +        EE+ +V+G
Sbjct: 409 PSYSTWEILTWAYLKRKDMEKVLDCFGKAIDSV--KKWTVNVRLVKGACKELEEQGNVKG 468

Query: 441 AEDFARILKRFKCLDASAYHLLLKTYAAAGKRAPDMRQRLIEDNIEVSSELEELL 494
           AE    +L++   ++   Y+ LL+TYA AG+ A  + +R+ +DN+E+  E +EL+
Sbjct: 469 AEKLMTLLQKAGYVNTQLYNSLLRTYAKAGEMALIVEERMAKDNVELDEETKELI 516

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7030878.11.8e-281100.00Pentatricopeptide repeat-containing protein, mitochondrial [Cucurbita argyrosper... [more]
KAG6600219.11.8e-27399.59Pentatricopeptide repeat-containing protein, mitochondrial, partial [Cucurbita a... [more]
XP_022942939.18.8e-27399.17pentatricopeptide repeat-containing protein At1g02370, mitochondrial-like isofor... [more]
XP_023512291.15.3e-27098.76pentatricopeptide repeat-containing protein At1g02370, mitochondrial-like isofor... [more]
XP_023005130.12.3e-26597.11pentatricopeptide repeat-containing protein At1g02370, mitochondrial-like isofor... [more]
Match NameE-valueIdentityDescription
Q9FZ244.3e-13753.43Pentatricopeptide repeat-containing protein At1g02370, mitochondrial OS=Arabidop... [more]
Q93WC51.9e-12148.16Pentatricopeptide repeat-containing protein At4g01990, mitochondrial OS=Arabidop... [more]
O227144.2e-10042.47Pentatricopeptide repeat-containing protein At1g60770 OS=Arabidopsis thaliana OX... [more]
Q8LPS69.3e-7635.59Pentatricopeptide repeat-containing protein At1g02150 OS=Arabidopsis thaliana OX... [more]
Q9SY077.9e-6734.32Pentatricopeptide repeat-containing protein At4g02820, mitochondrial OS=Arabidop... [more]
Match NameE-valueIdentityDescription
A0A6J1FST44.2e-27399.17pentatricopeptide repeat-containing protein At1g02370, mitochondrial-like isofor... [more]
A0A6J1KWK91.1e-26597.11pentatricopeptide repeat-containing protein At1g02370, mitochondrial-like isofor... [more]
A0A6J1CK872.1e-24388.24pentatricopeptide repeat-containing protein At1g02370, mitochondrial OS=Momordic... [more]
A0A6J1FX641.2e-22298.99pentatricopeptide repeat-containing protein At1g02370, mitochondrial-like isofor... [more]
A0A1S3BYD83.4e-22280.93pentatricopeptide repeat-containing protein At1g02370, mitochondrial-like isofor... [more]
Match NameE-valueIdentityDescription
AT1G02370.13.0e-13853.43Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT4G01990.11.4e-12248.16Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT1G60770.13.0e-10142.47Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT1G02150.16.6e-7735.59Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT4G02820.15.6e-6834.32Pentatricopeptide repeat (PPR) superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 475..495
NoneNo IPR availableCOILSCoilCoilcoord: 254..274
NoneNo IPR availablePANTHERPTHR45717OS12G0527900 PROTEINcoord: 4..495
NoneNo IPR availablePANTHERPTHR45717:SF8OS01G0301500 PROTEINcoord: 4..495
IPR002885Pentatricopeptide repeatPFAMPF13041PPR_2coord: 169..213
e-value: 1.3E-8
score: 34.9
IPR002885Pentatricopeptide repeatPFAMPF01535PPRcoord: 240..263
e-value: 1.4
score: 9.4
coord: 134..155
e-value: 0.034
score: 14.4
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 58..166
e-value: 1.2E-5
score: 26.8
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 167..386
e-value: 9.5E-17
score: 63.4
IPR011990Tetratricopeptide-like helical domain superfamilySUPERFAMILY48452TPR-likecoord: 135..321

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg08054-RACarg08054-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005739 mitochondrion
molecular_function GO:0005515 protein binding