Carg08012 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg08012
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionTrehalose 6-phosphate phosphatase
LocationCarg_Chr04: 1735668 .. 1738176 (-)
RNA-Seq ExpressionCarg08012
SyntenyCarg08012
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAGCTTTAAGATATCTAGAACTTTGCATGCCTATTCATGATGGAGTTTGATTTGGTTTTATGCACTTGTACGGCCACTTGAAATCTTACTGTTCTTCTCTGTTATATTATAGAAGCAGAGTACTCTGTTCCAACAAATCATGAGATCATATTTATTTTCCCAAAGCTTTACACTTAACTGTATAAACTCAAATTTTCAGGTGGTTCCAACTCTCAGTTTGTAAGGAGGACATAAGTGCACTTGGGCTTTGAATCAACCTCCAAAACTTGACCAAATGGATCTTAAACCAAACCATAATTCTCCCATTCTCTCTGACCCTGCGCCTATCACCAAGTCTAGACTTGGTGTACATTCGAATATGTTGCCATACTCAGCTGCTCCAGGAGTTGGGTTCGCTCCGAATTTGCTACTAATCACGAGGAAAAGGACCGGTGTGCTCGATGACGTTCGAGCAAGCATCTGGCTAGATGCTATGAAATCTTCATCTCCTCCTCCTAATAGGATTGCCAAGGATGTTGTCAATGAGCTTCCATCGTTTGATCCAGATCTTCTCTATTGCAGTTGGATGGTACTGTTTCTTTTACTGTCATGTTCTGATGTAGGATAAGTTTTATTGATCTTGGAGGAGTTTTTTATGTACAATATGCCCCTTTGTTACTTTCAGGTTAAGTATCCATCGGCACTTGCGTCGTTCGACCAAATCGTAACTCAGGCGAAAGGCAAAAGAATAGCATTGTTTCTGGACTATGATGGGACTCTTTCTCCAATTGTAGACAACCCTGATTGTGCATTCATGTCTGATACTGTAAGAATCTGTAATTCCTTGTAAATAAGTTGCTTAGAATAACTCCATCTTAGCTACTAACCTTGACTAACCTCTCTGCAAATCTCTTAGATGCGTGTGGCTGTAAAAGAGGCTGCAAAACATTTCCCGACTGCTATAATTAGTGGAAGAAGTCGTGACAAGGTATGATGCTTCATATATTATACAAATATTTGCCAATGAATCCGACTCGTTGATGTGGTCGTAAGTTTCGAGTTCCCTTGTTTTTGTTTGTTCAGGTTTATGAATTTATTGGACTAAATGAACTCTCCTATGCTGGTAGTCATGGAATGGACATTATGGTCTCTGATAAACACTCAACTAACGATCAGGTAACGAAATGGGAGTTGTTGTCGTTTTATCGACCGATGTCTAAATCTTTCTCGTTTTGGATGTTACTTCGACAGTGCTCTTACATACATTCCTTTCATTACATAGGGCAAGGAAGTTATGTTCCAGCCTGCTAGTGAATTTTTACCTCTGATTGACGAGGTACGAACCGACTTTTGGCGTACTAACATATATTCATATCGTGTTCGAGCATTTGAATGAAGAAAGTTCTCTTGTTTGGACAGGTTTACGAATCCCTGATTGAAATCGCCAAGGGAATTGCTGGAGCAAAAGTTGAAAACAATAAGTTCTGTGTGTCTGTTCATTACAGGAACGTAGATGATAAGGTACCTCCCTCTCAGATTGTCAATGGAGAATATGAATATTGAACAAATTTCATTTGATTGCTTTCTTCTCTCTAGGACTGGAATGCTCTTGCAAATCGTGTTTACGACCTTTTGGAAAACTACCCTCGACTGCGCGTTAGTCACGGTCGTAAGGTACTTCGATATCAGTAATGAAGGCTCAATCAAATTGTCATTAGATACTAACTTTTCCAAAGAACAATCTTCAATCTTGCTTGTATGTGTCTGATCGTCCTGTTTTGAATTCATAGGTTTTAGAAGTACGACCTGTGATTAGCTGGGACAAGGGCAAAGCTGTTACGTTCTTGCTTGAATCTCTTGGTAAGAAACACAATGTCCACAACCCTGGTTCCCATTTAAGAACATTCTTGCACATGTTGTTTAACCTCTTCAAACTGTACTCCAGGACTTAGCAACTGCGATGACGTGCTCCCAATCTACGTCGGAGATGACCGAACCGACGAAGATGCCTTCAGGGTAAGTTTATCATCTCATTAATTCCAGCACCCTTAATCTCTCCTCTGAGAAGCTCAATCTTAAGAAATGAAGCGACTTGAATTACAGGTTCTGAAAGAAAGAAATTGTGGCTATGGCATATTAGTCTCTTCAGTGCCAAAGGAAAGCAGTGCAGCCTCATACTCTTTGAGAGAACCATCTGAGGTACAGACTCTTTATATACTCAACCTGAACACCGCTCGAGACTGGGGAGATTCCATAACAGGATCATGAACCCAGTTTCGATTACCATGTCATGCTTTTAGTACCATTATCTCCTGCATTCGAGTCGTTTCTTCGAGGTGTGGTTTGAATCTGATTAGAGTTAGGATGTGATATGGAAAATGCAGGTGATGGAATTTCTGAAGTCATTGGTAACATGGAGGGCGTTGGAAGAGGGATTGAATGAATAGCAGAAATTGATATAACAATGGTATAGAGATAAGTATAGGGAAGGAAGGAAGGAAGGAAGGAAGGAGGGGAAGGTGAAGGAGG

mRNA sequence

AAAGCTTTAAGATATCTAGAACTTTGCATGCCTATTCATGATGGAGTTTGATTTGGTTTTATGCACTTGTACGGCCACTTGAAATCTTACTGTTCTTCTCTGTTATATTATAGAAGCAGAGTACTCTGTTCCAACAAATCATGAGATCATATTTATTTTCCCAAAGCTTTACACTTAACTGTATAAACTCAAATTTTCAGGTGGTTCCAACTCTCAGTTTGTAAGGAGGACATAAGTGCACTTGGGCTTTGAATCAACCTCCAAAACTTGACCAAATGGATCTTAAACCAAACCATAATTCTCCCATTCTCTCTGACCCTGCGCCTATCACCAAGTCTAGACTTGGTGTACATTCGAATATGTTGCCATACTCAGCTGCTCCAGGAGTTGGGTTCGCTCCGAATTTGCTACTAATCACGAGGAAAAGGACCGGTGTGCTCGATGACGTTCGAGCAAGCATCTGGCTAGATGCTATGAAATCTTCATCTCCTCCTCCTAATAGGATTGCCAAGGATGTTGTCAATGAGCTTCCATCGTTTGATCCAGATCTTCTCTATTGCAGTTGGATGGTTAAGTATCCATCGGCACTTGCGTCGTTCGACCAAATCGTAACTCAGGCGAAAGGCAAAAGAATAGCATTGTTTCTGGACTATGATGGGACTCTTTCTCCAATTGTAGACAACCCTGATTGTGCATTCATGTCTGATACTATGCGTGTGGCTGTAAAAGAGGCTGCAAAACATTTCCCGACTGCTATAATTAGTGGAAGAAGTCGTGACAAGGTTTATGAATTTATTGGACTAAATGAACTCTCCTATGCTGGTAGTCATGGAATGGACATTATGGTCTCTGATAAACACTCAACTAACGATCAGGGCAAGGAAGTTATGTTCCAGCCTGCTAGTGAATTTTTACCTCTGATTGACGAGGTTTACGAATCCCTGATTGAAATCGCCAAGGGAATTGCTGGAGCAAAAGTTGAAAACAATAAGTTCTGTGTGTCTGTTCATTACAGGAACGTAGATGATAAGGACTGGAATGCTCTTGCAAATCGTGTTTACGACCTTTTGGAAAACTACCCTCGACTGCGCGTTAGTCACGGTCGTAAGGTTTTAGAAGTACGACCTGTGATTAGCTGGGACAAGGGCAAAGCTGTTACGTTCTTGCTTGAATCTCTTGGACTTAGCAACTGCGATGACGTGCTCCCAATCTACGTCGGAGATGACCGAACCGACGAAGATGCCTTCAGGGTTCTGAAAGAAAGAAATTGTGGCTATGGCATATTAGTCTCTTCAGTGCCAAAGGAAAGCAGTGCAGCCTCATACTCTTTGAGAGAACCATCTGAGGTGATGGAATTTCTGAAGTCATTGGTAACATGGAGGGCGTTGGAAGAGGGATTGAATGAATAGCAGAAATTGATATAACAATGGTATAGAGATAAGTATAGGGAAGGAAGGAAGGAAGGAAGGAAGGAGGGGAAGGTGAAGGAGG

Coding sequence (CDS)

ATGGATCTTAAACCAAACCATAATTCTCCCATTCTCTCTGACCCTGCGCCTATCACCAAGTCTAGACTTGGTGTACATTCGAATATGTTGCCATACTCAGCTGCTCCAGGAGTTGGGTTCGCTCCGAATTTGCTACTAATCACGAGGAAAAGGACCGGTGTGCTCGATGACGTTCGAGCAAGCATCTGGCTAGATGCTATGAAATCTTCATCTCCTCCTCCTAATAGGATTGCCAAGGATGTTGTCAATGAGCTTCCATCGTTTGATCCAGATCTTCTCTATTGCAGTTGGATGGTTAAGTATCCATCGGCACTTGCGTCGTTCGACCAAATCGTAACTCAGGCGAAAGGCAAAAGAATAGCATTGTTTCTGGACTATGATGGGACTCTTTCTCCAATTGTAGACAACCCTGATTGTGCATTCATGTCTGATACTATGCGTGTGGCTGTAAAAGAGGCTGCAAAACATTTCCCGACTGCTATAATTAGTGGAAGAAGTCGTGACAAGGTTTATGAATTTATTGGACTAAATGAACTCTCCTATGCTGGTAGTCATGGAATGGACATTATGGTCTCTGATAAACACTCAACTAACGATCAGGGCAAGGAAGTTATGTTCCAGCCTGCTAGTGAATTTTTACCTCTGATTGACGAGGTTTACGAATCCCTGATTGAAATCGCCAAGGGAATTGCTGGAGCAAAAGTTGAAAACAATAAGTTCTGTGTGTCTGTTCATTACAGGAACGTAGATGATAAGGACTGGAATGCTCTTGCAAATCGTGTTTACGACCTTTTGGAAAACTACCCTCGACTGCGCGTTAGTCACGGTCGTAAGGTTTTAGAAGTACGACCTGTGATTAGCTGGGACAAGGGCAAAGCTGTTACGTTCTTGCTTGAATCTCTTGGACTTAGCAACTGCGATGACGTGCTCCCAATCTACGTCGGAGATGACCGAACCGACGAAGATGCCTTCAGGGTTCTGAAAGAAAGAAATTGTGGCTATGGCATATTAGTCTCTTCAGTGCCAAAGGAAAGCAGTGCAGCCTCATACTCTTTGAGAGAACCATCTGAGGTGATGGAATTTCTGAAGTCATTGGTAACATGGAGGGCGTTGGAAGAGGGATTGAATGAATAG

Protein sequence

MDLKPNHNSPILSDPAPITKSRLGVHSNMLPYSAAPGVGFAPNLLLITRKRTGVLDDVRASIWLDAMKSSSPPPNRIAKDVVNELPSFDPDLLYCSWMVKYPSALASFDQIVTQAKGKRIALFLDYDGTLSPIVDNPDCAFMSDTMRVAVKEAAKHFPTAIISGRSRDKVYEFIGLNELSYAGSHGMDIMVSDKHSTNDQGKEVMFQPASEFLPLIDEVYESLIEIAKGIAGAKVENNKFCVSVHYRNVDDKDWNALANRVYDLLENYPRLRVSHGRKVLEVRPVISWDKGKAVTFLLESLGLSNCDDVLPIYVGDDRTDEDAFRVLKERNCGYGILVSSVPKESSAASYSLREPSEVMEFLKSLVTWRALEEGLNE
Homology
BLAST of Carg08012 vs. NCBI nr
Match: KAG6600173.1 (Trehalose-phosphate phosphatase A, partial [Cucurbita argyrosperma subsp. sororia] >KAG7030836.1 Trehalose-phosphate phosphatase A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 764.6 bits (1973), Expect = 4.0e-217
Identity = 377/377 (100.00%), Postives = 377/377 (100.00%), Query Frame = 0

Query: 1   MDLKPNHNSPILSDPAPITKSRLGVHSNMLPYSAAPGVGFAPNLLLITRKRTGVLDDVRA 60
           MDLKPNHNSPILSDPAPITKSRLGVHSNMLPYSAAPGVGFAPNLLLITRKRTGVLDDVRA
Sbjct: 1   MDLKPNHNSPILSDPAPITKSRLGVHSNMLPYSAAPGVGFAPNLLLITRKRTGVLDDVRA 60

Query: 61  SIWLDAMKSSSPPPNRIAKDVVNELPSFDPDLLYCSWMVKYPSALASFDQIVTQAKGKRI 120
           SIWLDAMKSSSPPPNRIAKDVVNELPSFDPDLLYCSWMVKYPSALASFDQIVTQAKGKRI
Sbjct: 61  SIWLDAMKSSSPPPNRIAKDVVNELPSFDPDLLYCSWMVKYPSALASFDQIVTQAKGKRI 120

Query: 121 ALFLDYDGTLSPIVDNPDCAFMSDTMRVAVKEAAKHFPTAIISGRSRDKVYEFIGLNELS 180
           ALFLDYDGTLSPIVDNPDCAFMSDTMRVAVKEAAKHFPTAIISGRSRDKVYEFIGLNELS
Sbjct: 121 ALFLDYDGTLSPIVDNPDCAFMSDTMRVAVKEAAKHFPTAIISGRSRDKVYEFIGLNELS 180

Query: 181 YAGSHGMDIMVSDKHSTNDQGKEVMFQPASEFLPLIDEVYESLIEIAKGIAGAKVENNKF 240
           YAGSHGMDIMVSDKHSTNDQGKEVMFQPASEFLPLIDEVYESLIEIAKGIAGAKVENNKF
Sbjct: 181 YAGSHGMDIMVSDKHSTNDQGKEVMFQPASEFLPLIDEVYESLIEIAKGIAGAKVENNKF 240

Query: 241 CVSVHYRNVDDKDWNALANRVYDLLENYPRLRVSHGRKVLEVRPVISWDKGKAVTFLLES 300
           CVSVHYRNVDDKDWNALANRVYDLLENYPRLRVSHGRKVLEVRPVISWDKGKAVTFLLES
Sbjct: 241 CVSVHYRNVDDKDWNALANRVYDLLENYPRLRVSHGRKVLEVRPVISWDKGKAVTFLLES 300

Query: 301 LGLSNCDDVLPIYVGDDRTDEDAFRVLKERNCGYGILVSSVPKESSAASYSLREPSEVME 360
           LGLSNCDDVLPIYVGDDRTDEDAFRVLKERNCGYGILVSSVPKESSAASYSLREPSEVME
Sbjct: 301 LGLSNCDDVLPIYVGDDRTDEDAFRVLKERNCGYGILVSSVPKESSAASYSLREPSEVME 360

Query: 361 FLKSLVTWRALEEGLNE 378
           FLKSLVTWRALEEGLNE
Sbjct: 361 FLKSLVTWRALEEGLNE 377

BLAST of Carg08012 vs. NCBI nr
Match: XP_022941645.1 (trehalose-phosphate phosphatase A-like [Cucurbita moschata] >XP_022941646.1 trehalose-phosphate phosphatase A-like [Cucurbita moschata] >XP_022941647.1 trehalose-phosphate phosphatase A-like [Cucurbita moschata])

HSP 1 Score: 762.7 bits (1968), Expect = 1.5e-216
Identity = 376/377 (99.73%), Postives = 376/377 (99.73%), Query Frame = 0

Query: 1   MDLKPNHNSPILSDPAPITKSRLGVHSNMLPYSAAPGVGFAPNLLLITRKRTGVLDDVRA 60
           MDLKPNHNSPILSDPAPITKSRLGVHSNMLPYSAAPGVGFAPNLLLITRKRTGVLDDVRA
Sbjct: 1   MDLKPNHNSPILSDPAPITKSRLGVHSNMLPYSAAPGVGFAPNLLLITRKRTGVLDDVRA 60

Query: 61  SIWLDAMKSSSPPPNRIAKDVVNELPSFDPDLLYCSWMVKYPSALASFDQIVTQAKGKRI 120
           SIWLDAMKSSSPPPNRIAKDVVNELPSFDPDLLYCSWMVKYPSALASFDQIVTQAKGKRI
Sbjct: 61  SIWLDAMKSSSPPPNRIAKDVVNELPSFDPDLLYCSWMVKYPSALASFDQIVTQAKGKRI 120

Query: 121 ALFLDYDGTLSPIVDNPDCAFMSDTMRVAVKEAAKHFPTAIISGRSRDKVYEFIGLNELS 180
           ALFLDYDGTLSPIVDNPDCAFMSD MRVAVKEAAKHFPTAIISGRSRDKVYEFIGLNELS
Sbjct: 121 ALFLDYDGTLSPIVDNPDCAFMSDAMRVAVKEAAKHFPTAIISGRSRDKVYEFIGLNELS 180

Query: 181 YAGSHGMDIMVSDKHSTNDQGKEVMFQPASEFLPLIDEVYESLIEIAKGIAGAKVENNKF 240
           YAGSHGMDIMVSDKHSTNDQGKEVMFQPASEFLPLIDEVYESLIEIAKGIAGAKVENNKF
Sbjct: 181 YAGSHGMDIMVSDKHSTNDQGKEVMFQPASEFLPLIDEVYESLIEIAKGIAGAKVENNKF 240

Query: 241 CVSVHYRNVDDKDWNALANRVYDLLENYPRLRVSHGRKVLEVRPVISWDKGKAVTFLLES 300
           CVSVHYRNVDDKDWNALANRVYDLLENYPRLRVSHGRKVLEVRPVISWDKGKAVTFLLES
Sbjct: 241 CVSVHYRNVDDKDWNALANRVYDLLENYPRLRVSHGRKVLEVRPVISWDKGKAVTFLLES 300

Query: 301 LGLSNCDDVLPIYVGDDRTDEDAFRVLKERNCGYGILVSSVPKESSAASYSLREPSEVME 360
           LGLSNCDDVLPIYVGDDRTDEDAFRVLKERNCGYGILVSSVPKESSAASYSLREPSEVME
Sbjct: 301 LGLSNCDDVLPIYVGDDRTDEDAFRVLKERNCGYGILVSSVPKESSAASYSLREPSEVME 360

Query: 361 FLKSLVTWRALEEGLNE 378
           FLKSLVTWRALEEGLNE
Sbjct: 361 FLKSLVTWRALEEGLNE 377

BLAST of Carg08012 vs. NCBI nr
Match: XP_022966874.1 (trehalose-phosphate phosphatase A-like [Cucurbita maxima] >XP_022966947.1 trehalose-phosphate phosphatase A-like [Cucurbita maxima] >XP_022967031.1 trehalose-phosphate phosphatase A-like [Cucurbita maxima])

HSP 1 Score: 760.8 bits (1963), Expect = 5.7e-216
Identity = 375/377 (99.47%), Postives = 376/377 (99.73%), Query Frame = 0

Query: 1   MDLKPNHNSPILSDPAPITKSRLGVHSNMLPYSAAPGVGFAPNLLLITRKRTGVLDDVRA 60
           MDLKPNHNSPILSDPAPITKSRLGVHSNMLPYSAAPGVGFAPNLLLITRKRTGVL+DVRA
Sbjct: 1   MDLKPNHNSPILSDPAPITKSRLGVHSNMLPYSAAPGVGFAPNLLLITRKRTGVLNDVRA 60

Query: 61  SIWLDAMKSSSPPPNRIAKDVVNELPSFDPDLLYCSWMVKYPSALASFDQIVTQAKGKRI 120
           SIWLDAMKSSSPPPNRIAKDVVNELPSFDPDLLYCSWMVKYPSALASFDQIVTQAKGKRI
Sbjct: 61  SIWLDAMKSSSPPPNRIAKDVVNELPSFDPDLLYCSWMVKYPSALASFDQIVTQAKGKRI 120

Query: 121 ALFLDYDGTLSPIVDNPDCAFMSDTMRVAVKEAAKHFPTAIISGRSRDKVYEFIGLNELS 180
           ALFLDYDGTLSPIVDNPDCAFMSD MRVAVKEAAKHFPTAIISGRSRDKVYEFIGLNELS
Sbjct: 121 ALFLDYDGTLSPIVDNPDCAFMSDAMRVAVKEAAKHFPTAIISGRSRDKVYEFIGLNELS 180

Query: 181 YAGSHGMDIMVSDKHSTNDQGKEVMFQPASEFLPLIDEVYESLIEIAKGIAGAKVENNKF 240
           YAGSHGMDIMVSDKHSTNDQGKEVMFQPASEFLPLIDEVYESLIEIAKGIAGAKVENNKF
Sbjct: 181 YAGSHGMDIMVSDKHSTNDQGKEVMFQPASEFLPLIDEVYESLIEIAKGIAGAKVENNKF 240

Query: 241 CVSVHYRNVDDKDWNALANRVYDLLENYPRLRVSHGRKVLEVRPVISWDKGKAVTFLLES 300
           CVSVHYRNVDDKDWNALANRVYDLLENYPRLRVSHGRKVLEVRPVISWDKGKAVTFLLES
Sbjct: 241 CVSVHYRNVDDKDWNALANRVYDLLENYPRLRVSHGRKVLEVRPVISWDKGKAVTFLLES 300

Query: 301 LGLSNCDDVLPIYVGDDRTDEDAFRVLKERNCGYGILVSSVPKESSAASYSLREPSEVME 360
           LGLSNCDDVLPIYVGDDRTDEDAFRVLKERNCGYGILVSSVPKESSAASYSLREPSEVME
Sbjct: 301 LGLSNCDDVLPIYVGDDRTDEDAFRVLKERNCGYGILVSSVPKESSAASYSLREPSEVME 360

Query: 361 FLKSLVTWRALEEGLNE 378
           FLKSLVTWRALEEGLNE
Sbjct: 361 FLKSLVTWRALEEGLNE 377

BLAST of Carg08012 vs. NCBI nr
Match: XP_023525097.1 (trehalose-phosphate phosphatase A-like [Cucurbita pepo subsp. pepo] >XP_023525107.1 trehalose-phosphate phosphatase A-like [Cucurbita pepo subsp. pepo] >XP_023525116.1 trehalose-phosphate phosphatase A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 759.2 bits (1959), Expect = 1.7e-215
Identity = 374/377 (99.20%), Postives = 374/377 (99.20%), Query Frame = 0

Query: 1   MDLKPNHNSPILSDPAPITKSRLGVHSNMLPYSAAPGVGFAPNLLLITRKRTGVLDDVRA 60
           MDLKPNHNSPILSDPAPITKSRLGVHSNMLPYSAAPGVGFAPNLLLITRKRTGVLDDVRA
Sbjct: 1   MDLKPNHNSPILSDPAPITKSRLGVHSNMLPYSAAPGVGFAPNLLLITRKRTGVLDDVRA 60

Query: 61  SIWLDAMKSSSPPPNRIAKDVVNELPSFDPDLLYCSWMVKYPSALASFDQIVTQAKGKRI 120
           SIWLDAMKSSSPPPNRIAKDVVNELPSFDPDLLYCSWMVKYPSALASFDQIVTQAKGKRI
Sbjct: 61  SIWLDAMKSSSPPPNRIAKDVVNELPSFDPDLLYCSWMVKYPSALASFDQIVTQAKGKRI 120

Query: 121 ALFLDYDGTLSPIVDNPDCAFMSDTMRVAVKEAAKHFPTAIISGRSRDKVYEFIGLNELS 180
           ALFLDYDGTLSPIVDNPDCAFMSD MRVAVKEAAKHFPTAIISGRSRDKVYEFIGLNELS
Sbjct: 121 ALFLDYDGTLSPIVDNPDCAFMSDAMRVAVKEAAKHFPTAIISGRSRDKVYEFIGLNELS 180

Query: 181 YAGSHGMDIMVSDKHSTNDQGKEVMFQPASEFLPLIDEVYESLIEIAKGIAGAKVENNKF 240
           YAGSHGMDIMVSDKHSTNDQGKEVMFQPASEFLPLIDEVYESLIEIAKGI GAKVENNKF
Sbjct: 181 YAGSHGMDIMVSDKHSTNDQGKEVMFQPASEFLPLIDEVYESLIEIAKGITGAKVENNKF 240

Query: 241 CVSVHYRNVDDKDWNALANRVYDLLENYPRLRVSHGRKVLEVRPVISWDKGKAVTFLLES 300
           CVSVHYRNVDDKDWNALAN VYDLLENYPRLRVSHGRKVLEVRPVISWDKGKAVTFLLES
Sbjct: 241 CVSVHYRNVDDKDWNALANHVYDLLENYPRLRVSHGRKVLEVRPVISWDKGKAVTFLLES 300

Query: 301 LGLSNCDDVLPIYVGDDRTDEDAFRVLKERNCGYGILVSSVPKESSAASYSLREPSEVME 360
           LGLSNCDDVLPIYVGDDRTDEDAFRVLKERNCGYGILVSSVPKESSAASYSLREPSEVME
Sbjct: 301 LGLSNCDDVLPIYVGDDRTDEDAFRVLKERNCGYGILVSSVPKESSAASYSLREPSEVME 360

Query: 361 FLKSLVTWRALEEGLNE 378
           FLKSLVTWRALEEGLNE
Sbjct: 361 FLKSLVTWRALEEGLNE 377

BLAST of Carg08012 vs. NCBI nr
Match: XP_038877832.1 (trehalose-phosphate phosphatase A [Benincasa hispida] >XP_038877836.1 trehalose-phosphate phosphatase A [Benincasa hispida])

HSP 1 Score: 684.5 bits (1765), Expect = 5.2e-193
Identity = 335/369 (90.79%), Postives = 351/369 (95.12%), Query Frame = 0

Query: 1   MDLKPNHNSPILSDPAPITKSRLGVHSNMLPYSAAPGVGFAPNLLLITRKRTGVLDDVRA 60
           MDLK NHNSPIL+DPAPI KSRLGVHSNMLPY+ APGVGF+PNLLLI RK+TGVLDDVRA
Sbjct: 1   MDLKSNHNSPILTDPAPINKSRLGVHSNMLPYAPAPGVGFSPNLLLIPRKKTGVLDDVRA 60

Query: 61  SIWLDAMKSSSPPPNRIAKDVVNELPSFDPDLLYCSWMVKYPSALASFDQIVTQAKGKRI 120
           S WLDAMKSSSPPPNRI+KDVVNELPS DP  LYC+WM+KYPSALASFDQIVTQAKGKRI
Sbjct: 61  SSWLDAMKSSSPPPNRISKDVVNELPSIDPIFLYCNWMIKYPSALASFDQIVTQAKGKRI 120

Query: 121 ALFLDYDGTLSPIVDNPDCAFMSDTMRVAVKEAAKHFPTAIISGRSRDKVYEFIGLNELS 180
           ALFLDYDGTLSPIVDNPDCAFMSD MR AVKEAAK+FPTAIISGRSRDKVYEFIGL EL 
Sbjct: 121 ALFLDYDGTLSPIVDNPDCAFMSDAMRAAVKEAAKYFPTAIISGRSRDKVYEFIGLRELY 180

Query: 181 YAGSHGMDIMVSDKHSTNDQGKEVMFQPASEFLPLIDEVYESLIEIAKGIAGAKVENNKF 240
           YAGSHGMDIMVSD+HSTN+QGKEVMFQPASEFLPLIDEVY+SLIEI KGIAGAK+ENNKF
Sbjct: 181 YAGSHGMDIMVSDRHSTNNQGKEVMFQPASEFLPLIDEVYKSLIEITKGIAGAKIENNKF 240

Query: 241 CVSVHYRNVDDKDWNALANRVYDLLENYPRLRVSHGRKVLEVRPVISWDKGKAVTFLLES 300
           CVSVHYRNVDDK+WNALAN VYDLLE+YPRLRVSHGRKVLEVRPVISWDKGKAV FLLES
Sbjct: 241 CVSVHYRNVDDKNWNALANVVYDLLEDYPRLRVSHGRKVLEVRPVISWDKGKAVAFLLES 300

Query: 301 LGLSNCDDVLPIYVGDDRTDEDAFRVLKERNCGYGILVSSVPKESSAASYSLREPSEVME 360
           LGL NCD+VLPIYVGDDRTDEDAFRVLKERNCGYGILVSSV KESSAA YSLR+PSEVME
Sbjct: 301 LGLDNCDEVLPIYVGDDRTDEDAFRVLKERNCGYGILVSSVAKESSAA-YSLRDPSEVME 360

Query: 361 FLKSLVTWR 370
           FLKSLVTWR
Sbjct: 361 FLKSLVTWR 368

BLAST of Carg08012 vs. ExPASy Swiss-Prot
Match: O64896 (Trehalose-phosphate phosphatase A OS=Arabidopsis thaliana OX=3702 GN=TPPA PE=1 SV=1)

HSP 1 Score: 482.6 bits (1241), Expect = 3.9e-135
Identity = 246/385 (63.90%), Postives = 305/385 (79.22%), Query Frame = 0

Query: 1   MDLKPNHNSPILSDPAPITKSRLGVHSNMLPYSAAPGVGFAPN---LLLITRKRTGVLDD 60
           MD+K  H+SP+++D  PI+ SRL +  N LPYS+A     + N   LL + RK+TG+LDD
Sbjct: 1   MDMKSGHSSPVMTDSPPISNSRLTIRQNRLPYSSAAATAISQNNNLLLTVPRKKTGILDD 60

Query: 61  VRASIWLDAMKSSSPPPNRIAKDVVNELPSFDPDLLYCSWM-VKYPSALASFDQIVTQAK 120
           V+++ WLDAMKSSSPPP  + KD    L +   D+ Y  WM +KYPSAL SF++I++ AK
Sbjct: 61  VKSNGWLDAMKSSSPPPTILNKD---NLSNDATDMTYREWMQLKYPSALTSFEKIMSFAK 120

Query: 121 GKRIALFLDYDGTLSPIVDNPDCAFMSDTMRVAVKEAAKHFPTAIISGRSRDKVYEFIGL 180
           GKRIALFLDYDGTLSPIV+ PDCA+MS  MR AV+  AK+FPTAIISGRSRDKVYEF+ L
Sbjct: 121 GKRIALFLDYDGTLSPIVEEPDCAYMSSAMRSAVQNVAKYFPTAIISGRSRDKVYEFVNL 180

Query: 181 NELSYAGSHGMDIM------VSDKHSTN----DQGKEV-MFQPASEFLPLIDEVYESLIE 240
           +EL YAGSHGMDIM      ++ +HS      +QGK+V +FQPASEFLP+ID+V  SLIE
Sbjct: 181 SELYYAGSHGMDIMSPAGESLNHEHSRTVSVYEQGKDVNLFQPASEFLPMIDKVLCSLIE 240

Query: 241 IAKGIAGAKVENNKFCVSVHYRNVDDKDWNALANRVYDLLENYPRLRVSHGRKVLEVRPV 300
             K I G KVE+NKFC+SVHYRNV++K+W  +A  V D++  YP+LR++HGRKVLE+RPV
Sbjct: 241 STKDIKGVKVEDNKFCISVHYRNVEEKNWTLVAQCVDDVIRTYPKLRLTHGRKVLEIRPV 300

Query: 301 ISWDKGKAVTFLLESLGLSNCDDVLPIYVGDDRTDEDAFRVLKE-RNCGYGILVSSVPKE 360
           I WDKGKAVTFLLESLGL+NC+DVLPIYVGDDRTDEDAF+VL++  N GYGILVS+VPK+
Sbjct: 301 IDWDKGKAVTFLLESLGLNNCEDVLPIYVGDDRTDEDAFKVLRDGPNHGYGILVSAVPKD 360

Query: 361 SSAASYSLREPSEVMEFLKSLVTWR 370
           S+ A YSLR+PSEVMEFLKSLVTW+
Sbjct: 361 SN-AFYSLRDPSEVMEFLKSLVTWK 381

BLAST of Carg08012 vs. ExPASy Swiss-Prot
Match: Q9SU39 (Probable trehalose-phosphate phosphatase F OS=Arabidopsis thaliana OX=3702 GN=TPPF PE=2 SV=1)

HSP 1 Score: 449.5 bits (1155), Expect = 3.7e-125
Identity = 234/380 (61.58%), Postives = 284/380 (74.74%), Query Frame = 0

Query: 1   MDLKPNHNSPILSDPAP-ITKSRLGVHSNMLPYSAAPGVGFAPNLLLITRKRTGVLDDVR 60
           MDL  NH S +L DP+P + +SRLGV S                 ++   K+   LDDVR
Sbjct: 1   MDLNSNHKSSVLKDPSPSVNQSRLGVSS---------------RFMMSQWKKPAKLDDVR 60

Query: 61  ASIWLDAMKSSSPPPNRIAKDVVNELPSFDPDLLYCSWMVKYPSALASFDQIVTQAKGKR 120
           ++ WLDAM SSSPP  ++ KD   E+   D D    +WMVKYPSA++SF  I  QAK K+
Sbjct: 61  SNGWLDAMISSSPPRKKLVKDFNVEVAPED-DFAQRAWMVKYPSAISSFAHIAAQAKKKK 120

Query: 121 IALFLDYDGTLSPIVDNPDCAFMSDTMRVAVKEAAKHFPTAIISGRSRDKVYEFIGLNEL 180
           IA+FLDYDGTLSPIVD+PD A MSD MR AVK+ A +FPTAIISGRSRDKVY+ +GL EL
Sbjct: 121 IAVFLDYDGTLSPIVDDPDRAIMSDAMRSAVKDVASYFPTAIISGRSRDKVYQLVGLTEL 180

Query: 181 SYAGSHGMDIMVSDK-----HSTNDQGKEV-MFQPASEFLPLIDEVYESLIEIAKGIAGA 240
            YAGSHGMDIM S        ST+ QGKEV +FQPA EF+P+IDEV+ +L+E  K I GA
Sbjct: 181 YYAGSHGMDIMTSSDGPNCFKSTDQQGKEVNLFQPAREFIPVIDEVFRTLVEKMKDIKGA 240

Query: 241 KVENNKFCVSVHYRNVDDKDWNALANRVYDLLENYPRLRVSHGRKVLEVRPVISWDKGKA 300
           KVEN+KFC SVHYRNVD+KDW  +A RV+D L+ YPRLR++HGRKVLEVRPVI W+KG+A
Sbjct: 241 KVENHKFCASVHYRNVDEKDWPIIAQRVHDHLKQYPRLRLTHGRKVLEVRPVIDWNKGRA 300

Query: 301 VTFLLESLGLSNCDDVLPIYVGDDRTDEDAFRVLKERNCGYGILVSSVPKESSAASYSLR 360
           V FLLESLGLSN DD+LPIY+GDD TDEDAF+VL++ N G+GILVSS+PKES+ A YSLR
Sbjct: 301 VEFLLESLGLSNKDDLLPIYIGDDTTDEDAFKVLRDGNRGFGILVSSIPKESN-AFYSLR 360

Query: 361 EPSEVMEFLKSLVTWRALEE 374
           +PSEV +FLK+LV W  LE+
Sbjct: 361 DPSEVKKFLKTLVKWAKLEK 363

BLAST of Carg08012 vs. ExPASy Swiss-Prot
Match: Q9SUW0 (Probable trehalose-phosphate phosphatase G OS=Arabidopsis thaliana OX=3702 GN=TPPG PE=2 SV=1)

HSP 1 Score: 448.4 bits (1152), Expect = 8.2e-125
Identity = 236/385 (61.30%), Postives = 287/385 (74.55%), Query Frame = 0

Query: 1   MDLKPNHNSPILSDP-APITKSRLGVHSNMLPYSAAPGVGFAPNLLLITRKRTGVLDDVR 60
           MDL  N  +P+LSDP  P++K+RLG        S+ P   F  N    +RK+   LDDVR
Sbjct: 1   MDLNINKTTPVLSDPTTPVSKTRLG--------SSFPSGRFMMN----SRKKIPKLDDVR 60

Query: 61  ASIWLDAMKSSSPPPNRIAKDVVNELPSFDPDLLYCSWMVKYPSALASFDQIVTQAKGKR 120
           ++ WLDAM SSSPP  R+ KD   E+   D D    +WM+KYPSA+ SF  I  QAK K+
Sbjct: 61  SNGWLDAMISSSPPRKRLVKDFNIEIAPED-DFSQRAWMLKYPSAITSFAHIAAQAKNKK 120

Query: 121 IALFLDYDGTLSPIVDNPDCAFMSDTMRVAVKEAAKHFPTAIISGRSRDKVYEFIGLNEL 180
           IA+FLDYDGTLSPIVD+PD A MSD MR AVK+ AK+FPTAIISGRSRDKVY+ +GL EL
Sbjct: 121 IAVFLDYDGTLSPIVDDPDRAIMSDAMRAAVKDVAKYFPTAIISGRSRDKVYQLVGLTEL 180

Query: 181 SYAGSHGMDIMVSDK-----------HSTNDQGKEV-MFQPASEFLPLIDEVYESLIEIA 240
            YAGSHGMDIM                +T+ QG+EV +FQPA EF+P+I+EVY +L+EI 
Sbjct: 181 YYAGSHGMDIMTPVNPNGSPEDPNCIKTTDQQGEEVNLFQPAKEFIPVIEEVYNNLVEIT 240

Query: 241 KGIAGAKVENNKFCVSVHYRNVDDKDWNALANRVYDLLENYPRLRVSHGRKVLEVRPVIS 300
           K I GAKVEN+KFC SVHYRNVD+KDW  +A RV+D L+ YPRLR++HGRKVLEVRPVI 
Sbjct: 241 KCIKGAKVENHKFCTSVHYRNVDEKDWPLVAQRVHDHLKRYPRLRITHGRKVLEVRPVIE 300

Query: 301 WDKGKAVTFLLESLGLSNCDDVLPIYVGDDRTDEDAFRVLKERNCGYGILVSSVPKESSA 360
           W+KGKAV FLLESLGLSN D+ LPI++GDD+TDEDAF+VL+E N G+GILVSSVPKES+ 
Sbjct: 301 WNKGKAVEFLLESLGLSNNDEFLPIFIGDDKTDEDAFKVLREGNRGFGILVSSVPKESN- 360

Query: 361 ASYSLREPSEVMEFLKSLVTWRALE 373
           A YSLR+PSEV +FLK+LV W  +E
Sbjct: 361 AFYSLRDPSEVKKFLKTLVKWGKME 371

BLAST of Carg08012 vs. ExPASy Swiss-Prot
Match: Q9FWQ2 (Probable trehalose-phosphate phosphatase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=TPP2 PE=1 SV=1)

HSP 1 Score: 416.4 bits (1069), Expect = 3.5e-115
Identity = 225/385 (58.44%), Postives = 283/385 (73.51%), Query Frame = 0

Query: 1   MDLKPNHNSPILSDPAPITKSRLGVHSNMLPYSAAPGVGFAPNLLLITRKR---TGVLDD 60
           MDLK + NSP+++DP P    +L + S ++ Y+  P    +  L L T K+    G +++
Sbjct: 1   MDLKTS-NSPVIADPLP----KLALPSAVMTYT-TPTSFPSTGLYLNTPKKKPLPGKIEE 60

Query: 61  VRASIWLDAMKSSSPPPNRIAKDVVNELPSFDPDLLYCSWMVKYPSALASFDQIVTQAKG 120
           VRA+ WLD M +SSPP  R  KD  N++ + + DLLY +W+V +PSAL SF+ IV  A+G
Sbjct: 61  VRAAGWLDLMLASSPPRKRQTKDFANDVQADELDLLYRNWVVNHPSALTSFEDIVNLARG 120

Query: 121 KRIALFLDYDGTLSPIVDNPDCAFMSDTMRVAVKEAAKHFPTAIISGRSRDKVYEFIGLN 180
           KR+ALFLDYDGTLSPIVDNP+ A MSD MR AVK  A  FPTAIISGRSRDKV++F+ L 
Sbjct: 121 KRLALFLDYDGTLSPIVDNPENAVMSDEMRSAVKHVASLFPTAIISGRSRDKVFDFVKLT 180

Query: 181 ELSYAGSHGMDIM-------VSDKH-----STNDQGKEV-MFQPASEFLPLIDEVYESLI 240
           EL YAGSHGMDIM        S +H     ST+ +GKEV +FQPASEFLP+I EVY+ L 
Sbjct: 181 ELYYAGSHGMDIMGPVRKSDSSGQHVECIRSTDSEGKEVNLFQPASEFLPMISEVYKKLS 240

Query: 241 EIAKGIAGAKVENNKFCVSVHYRNVDDKDWNALANRVYDLLENYPRLRVSHGRKVLEVRP 300
           E  K I GA++E+NKFCVSVHYRNV   D+  +  RV  +L+NYP LR++HGRKVLEVRP
Sbjct: 241 ESIKDIDGARMEDNKFCVSVHYRNVAPHDYGEVHQRVTAVLKNYPCLRLTHGRKVLEVRP 300

Query: 301 VISWDKGKAVTFLLESLGLSNCDDVLPIYVGDDRTDEDAFRVLKERNCGYGILVSSVPKE 360
           VI W+KGKAV FLLESLGL   +DVLPIYVGDD+TDEDAF+VLK  + G+GILVSSVPK+
Sbjct: 301 VIDWNKGKAVEFLLESLGLCGKEDVLPIYVGDDKTDEDAFKVLKANSIGFGILVSSVPKD 360

Query: 361 SSAASYSLREPSEVMEFLKSLVTWR 370
           +  A YS+R+P+EVMEFLK L +W+
Sbjct: 361 TD-AFYSVRDPAEVMEFLKKLASWK 378

BLAST of Carg08012 vs. ExPASy Swiss-Prot
Match: Q75WV3 (Probable trehalose-phosphate phosphatase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=TPP1 PE=1 SV=1)

HSP 1 Score: 414.8 bits (1065), Expect = 1.0e-114
Identity = 215/373 (57.64%), Postives = 281/373 (75.34%), Query Frame = 0

Query: 1   MDLKPNHNSPILSDPAPITKSRL-GVHSNMLPYSAAPGVGFAPNLLLITRKRTGVLDDVR 60
           MDL  +++SP+++DP  I++  L G+ SN++ +S  PG G++ + + +   R  + ++V 
Sbjct: 1   MDL--SNSSPVITDPVAISQQLLGGLPSNLMQFSVMPG-GYSSSGMNVGVSRLKI-EEVL 60

Query: 61  ASIWLDAMKSSSPPPNRIAKDVVNELPSFDPDLLYCSWMVKYPSALASFDQIVTQAKGKR 120
            +  LDAMKSSS P  R+      +  S + D  Y +WM K PSALASF QIV  A+GK+
Sbjct: 61  VNGLLDAMKSSS-PRRRLNVAFGEDNSSEEEDPAYSAWMAKCPSALASFKQIVASAQGKK 120

Query: 121 IALFLDYDGTLSPIVDNPDCAFMSDTMRVAVKEAAKHFPTAIISGRSRDKVYEFIGLNEL 180
           IA+FLDYDGTLSPIVD+PD A MS  MR AV+  AK+FPTAI+SGRSR+KV+EF+ L EL
Sbjct: 121 IAVFLDYDGTLSPIVDDPDKAVMSPVMRAAVRNVAKYFPTAIVSGRSRNKVFEFVKLKEL 180

Query: 181 SYAGSHGMDIM---VSDKHSTNDQGKEVMFQPASEFLPLIDEVYESLIEIAKGIAGAKVE 240
            YAGSHGMDIM    + +HS     +  +FQPA +FLP+IDEV +SL+++  GI GA VE
Sbjct: 181 YYAGSHGMDIMAPSANHEHSAEKSKQANLFQPAHDFLPMIDEVTKSLLQVVSGIEGATVE 240

Query: 241 NNKFCVSVHYRNVDDKDWNALANRVYDLLENYPRLRVSHGRKVLEVRPVISWDKGKAVTF 300
           NNKFCVSVHYRNV +KDW  +A  V ++LE +PRL+V++GR VLEVRPVI WDKGKAV F
Sbjct: 241 NNKFCVSVHYRNVAEKDWKLVARLVNEVLEAFPRLKVTNGRMVLEVRPVIDWDKGKAVEF 300

Query: 301 LLESLGLSNCDDVLPIYVGDDRTDEDAFRVLKERNCGYGILVSSVPKESSAASYSLREPS 360
           LL+SLGL++ ++V+PIY+GDDRTDEDAF+VL++RNCGYGILVS VPKE+  A YSLR+PS
Sbjct: 301 LLQSLGLNDSENVIPIYIGDDRTDEDAFKVLRQRNCGYGILVSQVPKETE-AFYSLRDPS 360

Query: 361 EVMEFLKSLVTWR 370
           EVMEFL  LV W+
Sbjct: 361 EVMEFLNFLVRWK 367

BLAST of Carg08012 vs. ExPASy TrEMBL
Match: A0A6J1FLP0 (Trehalose 6-phosphate phosphatase OS=Cucurbita moschata OX=3662 GN=LOC111446945 PE=3 SV=1)

HSP 1 Score: 762.7 bits (1968), Expect = 7.3e-217
Identity = 376/377 (99.73%), Postives = 376/377 (99.73%), Query Frame = 0

Query: 1   MDLKPNHNSPILSDPAPITKSRLGVHSNMLPYSAAPGVGFAPNLLLITRKRTGVLDDVRA 60
           MDLKPNHNSPILSDPAPITKSRLGVHSNMLPYSAAPGVGFAPNLLLITRKRTGVLDDVRA
Sbjct: 1   MDLKPNHNSPILSDPAPITKSRLGVHSNMLPYSAAPGVGFAPNLLLITRKRTGVLDDVRA 60

Query: 61  SIWLDAMKSSSPPPNRIAKDVVNELPSFDPDLLYCSWMVKYPSALASFDQIVTQAKGKRI 120
           SIWLDAMKSSSPPPNRIAKDVVNELPSFDPDLLYCSWMVKYPSALASFDQIVTQAKGKRI
Sbjct: 61  SIWLDAMKSSSPPPNRIAKDVVNELPSFDPDLLYCSWMVKYPSALASFDQIVTQAKGKRI 120

Query: 121 ALFLDYDGTLSPIVDNPDCAFMSDTMRVAVKEAAKHFPTAIISGRSRDKVYEFIGLNELS 180
           ALFLDYDGTLSPIVDNPDCAFMSD MRVAVKEAAKHFPTAIISGRSRDKVYEFIGLNELS
Sbjct: 121 ALFLDYDGTLSPIVDNPDCAFMSDAMRVAVKEAAKHFPTAIISGRSRDKVYEFIGLNELS 180

Query: 181 YAGSHGMDIMVSDKHSTNDQGKEVMFQPASEFLPLIDEVYESLIEIAKGIAGAKVENNKF 240
           YAGSHGMDIMVSDKHSTNDQGKEVMFQPASEFLPLIDEVYESLIEIAKGIAGAKVENNKF
Sbjct: 181 YAGSHGMDIMVSDKHSTNDQGKEVMFQPASEFLPLIDEVYESLIEIAKGIAGAKVENNKF 240

Query: 241 CVSVHYRNVDDKDWNALANRVYDLLENYPRLRVSHGRKVLEVRPVISWDKGKAVTFLLES 300
           CVSVHYRNVDDKDWNALANRVYDLLENYPRLRVSHGRKVLEVRPVISWDKGKAVTFLLES
Sbjct: 241 CVSVHYRNVDDKDWNALANRVYDLLENYPRLRVSHGRKVLEVRPVISWDKGKAVTFLLES 300

Query: 301 LGLSNCDDVLPIYVGDDRTDEDAFRVLKERNCGYGILVSSVPKESSAASYSLREPSEVME 360
           LGLSNCDDVLPIYVGDDRTDEDAFRVLKERNCGYGILVSSVPKESSAASYSLREPSEVME
Sbjct: 301 LGLSNCDDVLPIYVGDDRTDEDAFRVLKERNCGYGILVSSVPKESSAASYSLREPSEVME 360

Query: 361 FLKSLVTWRALEEGLNE 378
           FLKSLVTWRALEEGLNE
Sbjct: 361 FLKSLVTWRALEEGLNE 377

BLAST of Carg08012 vs. ExPASy TrEMBL
Match: A0A6J1HQX9 (Trehalose 6-phosphate phosphatase OS=Cucurbita maxima OX=3661 GN=LOC111466421 PE=3 SV=1)

HSP 1 Score: 760.8 bits (1963), Expect = 2.8e-216
Identity = 375/377 (99.47%), Postives = 376/377 (99.73%), Query Frame = 0

Query: 1   MDLKPNHNSPILSDPAPITKSRLGVHSNMLPYSAAPGVGFAPNLLLITRKRTGVLDDVRA 60
           MDLKPNHNSPILSDPAPITKSRLGVHSNMLPYSAAPGVGFAPNLLLITRKRTGVL+DVRA
Sbjct: 1   MDLKPNHNSPILSDPAPITKSRLGVHSNMLPYSAAPGVGFAPNLLLITRKRTGVLNDVRA 60

Query: 61  SIWLDAMKSSSPPPNRIAKDVVNELPSFDPDLLYCSWMVKYPSALASFDQIVTQAKGKRI 120
           SIWLDAMKSSSPPPNRIAKDVVNELPSFDPDLLYCSWMVKYPSALASFDQIVTQAKGKRI
Sbjct: 61  SIWLDAMKSSSPPPNRIAKDVVNELPSFDPDLLYCSWMVKYPSALASFDQIVTQAKGKRI 120

Query: 121 ALFLDYDGTLSPIVDNPDCAFMSDTMRVAVKEAAKHFPTAIISGRSRDKVYEFIGLNELS 180
           ALFLDYDGTLSPIVDNPDCAFMSD MRVAVKEAAKHFPTAIISGRSRDKVYEFIGLNELS
Sbjct: 121 ALFLDYDGTLSPIVDNPDCAFMSDAMRVAVKEAAKHFPTAIISGRSRDKVYEFIGLNELS 180

Query: 181 YAGSHGMDIMVSDKHSTNDQGKEVMFQPASEFLPLIDEVYESLIEIAKGIAGAKVENNKF 240
           YAGSHGMDIMVSDKHSTNDQGKEVMFQPASEFLPLIDEVYESLIEIAKGIAGAKVENNKF
Sbjct: 181 YAGSHGMDIMVSDKHSTNDQGKEVMFQPASEFLPLIDEVYESLIEIAKGIAGAKVENNKF 240

Query: 241 CVSVHYRNVDDKDWNALANRVYDLLENYPRLRVSHGRKVLEVRPVISWDKGKAVTFLLES 300
           CVSVHYRNVDDKDWNALANRVYDLLENYPRLRVSHGRKVLEVRPVISWDKGKAVTFLLES
Sbjct: 241 CVSVHYRNVDDKDWNALANRVYDLLENYPRLRVSHGRKVLEVRPVISWDKGKAVTFLLES 300

Query: 301 LGLSNCDDVLPIYVGDDRTDEDAFRVLKERNCGYGILVSSVPKESSAASYSLREPSEVME 360
           LGLSNCDDVLPIYVGDDRTDEDAFRVLKERNCGYGILVSSVPKESSAASYSLREPSEVME
Sbjct: 301 LGLSNCDDVLPIYVGDDRTDEDAFRVLKERNCGYGILVSSVPKESSAASYSLREPSEVME 360

Query: 361 FLKSLVTWRALEEGLNE 378
           FLKSLVTWRALEEGLNE
Sbjct: 361 FLKSLVTWRALEEGLNE 377

BLAST of Carg08012 vs. ExPASy TrEMBL
Match: A0A6J1J8A8 (Trehalose 6-phosphate phosphatase OS=Cucurbita maxima OX=3661 GN=LOC111483480 PE=3 SV=1)

HSP 1 Score: 682.2 bits (1759), Expect = 1.3e-192
Identity = 334/369 (90.51%), Postives = 352/369 (95.39%), Query Frame = 0

Query: 1   MDLKPNHNSPILSDPAPITKSRLGVHSNMLPYSAAPGVGFAPNLLLITRKRTGVLDDVRA 60
           MDLK NHNSPIL+DPAPI+KSRLGVHSNMLPY+AAPGVGF+PNLLLI RK+TGVLDDVRA
Sbjct: 1   MDLKSNHNSPILTDPAPISKSRLGVHSNMLPYAAAPGVGFSPNLLLIPRKKTGVLDDVRA 60

Query: 61  SIWLDAMKSSSPPPNRIAKDVVNELPSFDPDLLYCSWMVKYPSALASFDQIVTQAKGKRI 120
           S WLDAMKSSSPPPNRI+KDVVNELPSFDPD LY +WMVKYPSAL SF+QIVT AKGKRI
Sbjct: 61  SCWLDAMKSSSPPPNRISKDVVNELPSFDPDFLYRNWMVKYPSALGSFEQIVTHAKGKRI 120

Query: 121 ALFLDYDGTLSPIVDNPDCAFMSDTMRVAVKEAAKHFPTAIISGRSRDKVYEFIGLNELS 180
            LFLDYDGTLSPIVDNPDCAFMSD MR AVKEAAK+FPTAIISGRSRDKVYEFIGL EL 
Sbjct: 121 VLFLDYDGTLSPIVDNPDCAFMSDAMRAAVKEAAKYFPTAIISGRSRDKVYEFIGLRELY 180

Query: 181 YAGSHGMDIMVSDKHSTNDQGKEVMFQPASEFLPLIDEVYESLIEIAKGIAGAKVENNKF 240
           YAGSHGMDIMVSD+HSTN+QGKEVMFQPASEFLPLIDEVY+SLIEI +GIAGAKVENNKF
Sbjct: 181 YAGSHGMDIMVSDRHSTNNQGKEVMFQPASEFLPLIDEVYKSLIEITRGIAGAKVENNKF 240

Query: 241 CVSVHYRNVDDKDWNALANRVYDLLENYPRLRVSHGRKVLEVRPVISWDKGKAVTFLLES 300
           CVSVHYRNVDDK+WNALAN VYDLLE+YPRLRVSHGRKVLEVRPVISWDKGKAVTFLLES
Sbjct: 241 CVSVHYRNVDDKNWNALANCVYDLLEDYPRLRVSHGRKVLEVRPVISWDKGKAVTFLLES 300

Query: 301 LGLSNCDDVLPIYVGDDRTDEDAFRVLKERNCGYGILVSSVPKESSAASYSLREPSEVME 360
           LGL+NCD+VLPIYVGDDRTDEDAFR LKERNCGYGILVSSV KESSAA YSLR+PSEVME
Sbjct: 301 LGLNNCDEVLPIYVGDDRTDEDAFRALKERNCGYGILVSSVAKESSAA-YSLRDPSEVME 360

Query: 361 FLKSLVTWR 370
           FLKSLVTWR
Sbjct: 361 FLKSLVTWR 368

BLAST of Carg08012 vs. ExPASy TrEMBL
Match: A0A6J1E8M3 (Trehalose 6-phosphate phosphatase OS=Cucurbita moschata OX=3662 GN=LOC111430866 PE=3 SV=1)

HSP 1 Score: 680.2 bits (1754), Expect = 4.8e-192
Identity = 333/369 (90.24%), Postives = 352/369 (95.39%), Query Frame = 0

Query: 1   MDLKPNHNSPILSDPAPITKSRLGVHSNMLPYSAAPGVGFAPNLLLITRKRTGVLDDVRA 60
           MDLK NHNSPIL+DPAPI+KSRLGVHSNMLPY+AAPGVGF+PNLLLI RK+TGVLDDVRA
Sbjct: 1   MDLKSNHNSPILTDPAPISKSRLGVHSNMLPYAAAPGVGFSPNLLLIPRKKTGVLDDVRA 60

Query: 61  SIWLDAMKSSSPPPNRIAKDVVNELPSFDPDLLYCSWMVKYPSALASFDQIVTQAKGKRI 120
           S WLDAMKSSSPPPNRI+KDVVNELPSFDPD LY +WMVKYPSAL SF+QIVT AKGKRI
Sbjct: 61  SCWLDAMKSSSPPPNRISKDVVNELPSFDPDFLYRNWMVKYPSALGSFEQIVTHAKGKRI 120

Query: 121 ALFLDYDGTLSPIVDNPDCAFMSDTMRVAVKEAAKHFPTAIISGRSRDKVYEFIGLNELS 180
            LFLDYDGTLSPIVDNPDCAFMSD MR AVKEAAK+FPTAIISGRSRDKV+EFIGL EL 
Sbjct: 121 VLFLDYDGTLSPIVDNPDCAFMSDAMRAAVKEAAKYFPTAIISGRSRDKVFEFIGLRELY 180

Query: 181 YAGSHGMDIMVSDKHSTNDQGKEVMFQPASEFLPLIDEVYESLIEIAKGIAGAKVENNKF 240
           YAGSHGMDIMVSD+HSTN+QGKEVMFQPASEFLPLIDEV++SLIEI KGIAGAKVENNKF
Sbjct: 181 YAGSHGMDIMVSDRHSTNNQGKEVMFQPASEFLPLIDEVFKSLIEITKGIAGAKVENNKF 240

Query: 241 CVSVHYRNVDDKDWNALANRVYDLLENYPRLRVSHGRKVLEVRPVISWDKGKAVTFLLES 300
           CVSVHYRNVDDK+WNALAN VYDLLE+YPRLRVSHGRKVLEVRPVISWDKGKAVTFLLES
Sbjct: 241 CVSVHYRNVDDKNWNALANCVYDLLEDYPRLRVSHGRKVLEVRPVISWDKGKAVTFLLES 300

Query: 301 LGLSNCDDVLPIYVGDDRTDEDAFRVLKERNCGYGILVSSVPKESSAASYSLREPSEVME 360
           LGL+NCD+VLPIYVGDDRTDEDAFR LKERNCGYGILVSSV KESSAA YSLR+PSEVME
Sbjct: 301 LGLNNCDEVLPIYVGDDRTDEDAFRALKERNCGYGILVSSVAKESSAA-YSLRDPSEVME 360

Query: 361 FLKSLVTWR 370
           FLKSLVTWR
Sbjct: 361 FLKSLVTWR 368

BLAST of Carg08012 vs. ExPASy TrEMBL
Match: A0A1S3AYK7 (Trehalose 6-phosphate phosphatase OS=Cucumis melo OX=3656 GN=LOC103483982 PE=3 SV=1)

HSP 1 Score: 666.0 bits (1717), Expect = 9.3e-188
Identity = 326/369 (88.35%), Postives = 348/369 (94.31%), Query Frame = 0

Query: 1   MDLKPNHNSPILSDPAPITKSRLGVHSNMLPYSAAPGVGFAPNLLLITRKRTGVLDDVRA 60
           MDLK NHNSPIL+DPAPI+KSRLGVH NMLPY++APGVGF+PN+LLI RK+TGVLDDVRA
Sbjct: 1   MDLKSNHNSPILTDPAPISKSRLGVHLNMLPYASAPGVGFSPNMLLIPRKKTGVLDDVRA 60

Query: 61  SIWLDAMKSSSPPPNRIAKDVVNELPSFDPDLLYCSWMVKYPSALASFDQIVTQAKGKRI 120
           S WLDAMKSSSPPPNRI+KD VNEL S DPD LY +WM+KYPSALASFDQIV  AKGKRI
Sbjct: 61  SSWLDAMKSSSPPPNRISKDDVNELSSLDPDFLYRNWMIKYPSALASFDQIVPHAKGKRI 120

Query: 121 ALFLDYDGTLSPIVDNPDCAFMSDTMRVAVKEAAKHFPTAIISGRSRDKVYEFIGLNELS 180
           ALFLDYDGTLSPIVDNPD AFMSD MR  VKEAAK+FPTAIISGRSRDKVYEFIGL+EL 
Sbjct: 121 ALFLDYDGTLSPIVDNPDGAFMSDAMRATVKEAAKYFPTAIISGRSRDKVYEFIGLSELH 180

Query: 181 YAGSHGMDIMVSDKHSTNDQGKEVMFQPASEFLPLIDEVYESLIEIAKGIAGAKVENNKF 240
           YAGSHGMDIMVSD+HST+DQGKEVMFQPASEFLPLIDEVY+SLIEI KGIAGAKVENNKF
Sbjct: 181 YAGSHGMDIMVSDRHSTDDQGKEVMFQPASEFLPLIDEVYKSLIEITKGIAGAKVENNKF 240

Query: 241 CVSVHYRNVDDKDWNALANRVYDLLENYPRLRVSHGRKVLEVRPVISWDKGKAVTFLLES 300
           CVSVHYRNVDDK+WNALAN VYDLLE+YPRLRVSHGRKVLEVRPVISWDKGKAV FLLE+
Sbjct: 241 CVSVHYRNVDDKNWNALANSVYDLLEDYPRLRVSHGRKVLEVRPVISWDKGKAVAFLLET 300

Query: 301 LGLSNCDDVLPIYVGDDRTDEDAFRVLKERNCGYGILVSSVPKESSAASYSLREPSEVME 360
           LGL+NCD+VLPIY+GDDRTDEDAFRVLKERNCGYGILVSSV KESSAA YSLR+PSEVME
Sbjct: 301 LGLNNCDEVLPIYIGDDRTDEDAFRVLKERNCGYGILVSSVAKESSAA-YSLRDPSEVME 360

Query: 361 FLKSLVTWR 370
           FLKSLVTWR
Sbjct: 361 FLKSLVTWR 368

BLAST of Carg08012 vs. TAIR 10
Match: AT5G51460.2 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 487.3 bits (1253), Expect = 1.1e-137
Identity = 246/384 (64.06%), Postives = 305/384 (79.43%), Query Frame = 0

Query: 1   MDLKPNHNSPILSDPAPITKSRLGVHSNMLPYSAAPGVGFAPN---LLLITRKRTGVLDD 60
           MD+K  H+SP+++D  PI+ SRL +  N LPYS+A     + N   LL + RK+TG+LDD
Sbjct: 1   MDMKSGHSSPVMTDSPPISNSRLTIRQNRLPYSSAAATAISQNNNLLLTVPRKKTGILDD 60

Query: 61  VRASIWLDAMKSSSPPPNRIAKDVVNELPSFDPDLLYCSWMVKYPSALASFDQIVTQAKG 120
           V+++ WLDAMKSSSPPP  + KD    L +   D+ Y  WM+KYPSAL SF++I++ AKG
Sbjct: 61  VKSNGWLDAMKSSSPPPTILNKD---NLSNDATDMTYREWMLKYPSALTSFEKIMSFAKG 120

Query: 121 KRIALFLDYDGTLSPIVDNPDCAFMSDTMRVAVKEAAKHFPTAIISGRSRDKVYEFIGLN 180
           KRIALFLDYDGTLSPIV+ PDCA+MS  MR AV+  AK+FPTAIISGRSRDKVYEF+ L+
Sbjct: 121 KRIALFLDYDGTLSPIVEEPDCAYMSSAMRSAVQNVAKYFPTAIISGRSRDKVYEFVNLS 180

Query: 181 ELSYAGSHGMDIM------VSDKHSTN----DQGKEV-MFQPASEFLPLIDEVYESLIEI 240
           EL YAGSHGMDIM      ++ +HS      +QGK+V +FQPASEFLP+ID+V  SLIE 
Sbjct: 181 ELYYAGSHGMDIMSPAGESLNHEHSRTVSVYEQGKDVNLFQPASEFLPMIDKVLCSLIES 240

Query: 241 AKGIAGAKVENNKFCVSVHYRNVDDKDWNALANRVYDLLENYPRLRVSHGRKVLEVRPVI 300
            K I G KVE+NKFC+SVHYRNV++K+W  +A  V D++  YP+LR++HGRKVLE+RPVI
Sbjct: 241 TKDIKGVKVEDNKFCISVHYRNVEEKNWTLVAQCVDDVIRTYPKLRLTHGRKVLEIRPVI 300

Query: 301 SWDKGKAVTFLLESLGLSNCDDVLPIYVGDDRTDEDAFRVLKE-RNCGYGILVSSVPKES 360
            WDKGKAVTFLLESLGL+NC+DVLPIYVGDDRTDEDAF+VL++  N GYGILVS+VPK+S
Sbjct: 301 DWDKGKAVTFLLESLGLNNCEDVLPIYVGDDRTDEDAFKVLRDGPNHGYGILVSAVPKDS 360

Query: 361 SAASYSLREPSEVMEFLKSLVTWR 370
           + A YSLR+PSEVMEFLKSLVTW+
Sbjct: 361 N-AFYSLRDPSEVMEFLKSLVTWK 380

BLAST of Carg08012 vs. TAIR 10
Match: AT5G51460.3 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 482.6 bits (1241), Expect = 2.8e-136
Identity = 246/385 (63.90%), Postives = 305/385 (79.22%), Query Frame = 0

Query: 1   MDLKPNHNSPILSDPAPITKSRLGVHSNMLPYSAAPGVGFAPN---LLLITRKRTGVLDD 60
           MD+K  H+SP+++D  PI+ SRL +  N LPYS+A     + N   LL + RK+TG+LDD
Sbjct: 1   MDMKSGHSSPVMTDSPPISNSRLTIRQNRLPYSSAAATAISQNNNLLLTVPRKKTGILDD 60

Query: 61  VRASIWLDAMKSSSPPPNRIAKDVVNELPSFDPDLLYCSWM-VKYPSALASFDQIVTQAK 120
           V+++ WLDAMKSSSPPP  + KD    L +   D+ Y  WM +KYPSAL SF++I++ AK
Sbjct: 61  VKSNGWLDAMKSSSPPPTILNKD---NLSNDATDMTYREWMQLKYPSALTSFEKIMSFAK 120

Query: 121 GKRIALFLDYDGTLSPIVDNPDCAFMSDTMRVAVKEAAKHFPTAIISGRSRDKVYEFIGL 180
           GKRIALFLDYDGTLSPIV+ PDCA+MS  MR AV+  AK+FPTAIISGRSRDKVYEF+ L
Sbjct: 121 GKRIALFLDYDGTLSPIVEEPDCAYMSSAMRSAVQNVAKYFPTAIISGRSRDKVYEFVNL 180

Query: 181 NELSYAGSHGMDIM------VSDKHSTN----DQGKEV-MFQPASEFLPLIDEVYESLIE 240
           +EL YAGSHGMDIM      ++ +HS      +QGK+V +FQPASEFLP+ID+V  SLIE
Sbjct: 181 SELYYAGSHGMDIMSPAGESLNHEHSRTVSVYEQGKDVNLFQPASEFLPMIDKVLCSLIE 240

Query: 241 IAKGIAGAKVENNKFCVSVHYRNVDDKDWNALANRVYDLLENYPRLRVSHGRKVLEVRPV 300
             K I G KVE+NKFC+SVHYRNV++K+W  +A  V D++  YP+LR++HGRKVLE+RPV
Sbjct: 241 STKDIKGVKVEDNKFCISVHYRNVEEKNWTLVAQCVDDVIRTYPKLRLTHGRKVLEIRPV 300

Query: 301 ISWDKGKAVTFLLESLGLSNCDDVLPIYVGDDRTDEDAFRVLKE-RNCGYGILVSSVPKE 360
           I WDKGKAVTFLLESLGL+NC+DVLPIYVGDDRTDEDAF+VL++  N GYGILVS+VPK+
Sbjct: 301 IDWDKGKAVTFLLESLGLNNCEDVLPIYVGDDRTDEDAFKVLRDGPNHGYGILVSAVPKD 360

Query: 361 SSAASYSLREPSEVMEFLKSLVTWR 370
           S+ A YSLR+PSEVMEFLKSLVTW+
Sbjct: 361 SN-AFYSLRDPSEVMEFLKSLVTWK 381

BLAST of Carg08012 vs. TAIR 10
Match: AT5G51460.1 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 482.6 bits (1241), Expect = 2.8e-136
Identity = 246/385 (63.90%), Postives = 305/385 (79.22%), Query Frame = 0

Query: 1   MDLKPNHNSPILSDPAPITKSRLGVHSNMLPYSAAPGVGFAPN---LLLITRKRTGVLDD 60
           MD+K  H+SP+++D  PI+ SRL +  N LPYS+A     + N   LL + RK+TG+LDD
Sbjct: 1   MDMKSGHSSPVMTDSPPISNSRLTIRQNRLPYSSAAATAISQNNNLLLTVPRKKTGILDD 60

Query: 61  VRASIWLDAMKSSSPPPNRIAKDVVNELPSFDPDLLYCSWM-VKYPSALASFDQIVTQAK 120
           V+++ WLDAMKSSSPPP  + KD    L +   D+ Y  WM +KYPSAL SF++I++ AK
Sbjct: 61  VKSNGWLDAMKSSSPPPTILNKD---NLSNDATDMTYREWMQLKYPSALTSFEKIMSFAK 120

Query: 121 GKRIALFLDYDGTLSPIVDNPDCAFMSDTMRVAVKEAAKHFPTAIISGRSRDKVYEFIGL 180
           GKRIALFLDYDGTLSPIV+ PDCA+MS  MR AV+  AK+FPTAIISGRSRDKVYEF+ L
Sbjct: 121 GKRIALFLDYDGTLSPIVEEPDCAYMSSAMRSAVQNVAKYFPTAIISGRSRDKVYEFVNL 180

Query: 181 NELSYAGSHGMDIM------VSDKHSTN----DQGKEV-MFQPASEFLPLIDEVYESLIE 240
           +EL YAGSHGMDIM      ++ +HS      +QGK+V +FQPASEFLP+ID+V  SLIE
Sbjct: 181 SELYYAGSHGMDIMSPAGESLNHEHSRTVSVYEQGKDVNLFQPASEFLPMIDKVLCSLIE 240

Query: 241 IAKGIAGAKVENNKFCVSVHYRNVDDKDWNALANRVYDLLENYPRLRVSHGRKVLEVRPV 300
             K I G KVE+NKFC+SVHYRNV++K+W  +A  V D++  YP+LR++HGRKVLE+RPV
Sbjct: 241 STKDIKGVKVEDNKFCISVHYRNVEEKNWTLVAQCVDDVIRTYPKLRLTHGRKVLEIRPV 300

Query: 301 ISWDKGKAVTFLLESLGLSNCDDVLPIYVGDDRTDEDAFRVLKE-RNCGYGILVSSVPKE 360
           I WDKGKAVTFLLESLGL+NC+DVLPIYVGDDRTDEDAF+VL++  N GYGILVS+VPK+
Sbjct: 301 IDWDKGKAVTFLLESLGLNNCEDVLPIYVGDDRTDEDAFKVLRDGPNHGYGILVSAVPKD 360

Query: 361 SSAASYSLREPSEVMEFLKSLVTWR 370
           S+ A YSLR+PSEVMEFLKSLVTW+
Sbjct: 361 SN-AFYSLRDPSEVMEFLKSLVTWK 381

BLAST of Carg08012 vs. TAIR 10
Match: AT4G12430.1 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 449.5 bits (1155), Expect = 2.6e-126
Identity = 234/380 (61.58%), Postives = 284/380 (74.74%), Query Frame = 0

Query: 1   MDLKPNHNSPILSDPAP-ITKSRLGVHSNMLPYSAAPGVGFAPNLLLITRKRTGVLDDVR 60
           MDL  NH S +L DP+P + +SRLGV S                 ++   K+   LDDVR
Sbjct: 1   MDLNSNHKSSVLKDPSPSVNQSRLGVSS---------------RFMMSQWKKPAKLDDVR 60

Query: 61  ASIWLDAMKSSSPPPNRIAKDVVNELPSFDPDLLYCSWMVKYPSALASFDQIVTQAKGKR 120
           ++ WLDAM SSSPP  ++ KD   E+   D D    +WMVKYPSA++SF  I  QAK K+
Sbjct: 61  SNGWLDAMISSSPPRKKLVKDFNVEVAPED-DFAQRAWMVKYPSAISSFAHIAAQAKKKK 120

Query: 121 IALFLDYDGTLSPIVDNPDCAFMSDTMRVAVKEAAKHFPTAIISGRSRDKVYEFIGLNEL 180
           IA+FLDYDGTLSPIVD+PD A MSD MR AVK+ A +FPTAIISGRSRDKVY+ +GL EL
Sbjct: 121 IAVFLDYDGTLSPIVDDPDRAIMSDAMRSAVKDVASYFPTAIISGRSRDKVYQLVGLTEL 180

Query: 181 SYAGSHGMDIMVSDK-----HSTNDQGKEV-MFQPASEFLPLIDEVYESLIEIAKGIAGA 240
            YAGSHGMDIM S        ST+ QGKEV +FQPA EF+P+IDEV+ +L+E  K I GA
Sbjct: 181 YYAGSHGMDIMTSSDGPNCFKSTDQQGKEVNLFQPAREFIPVIDEVFRTLVEKMKDIKGA 240

Query: 241 KVENNKFCVSVHYRNVDDKDWNALANRVYDLLENYPRLRVSHGRKVLEVRPVISWDKGKA 300
           KVEN+KFC SVHYRNVD+KDW  +A RV+D L+ YPRLR++HGRKVLEVRPVI W+KG+A
Sbjct: 241 KVENHKFCASVHYRNVDEKDWPIIAQRVHDHLKQYPRLRLTHGRKVLEVRPVIDWNKGRA 300

Query: 301 VTFLLESLGLSNCDDVLPIYVGDDRTDEDAFRVLKERNCGYGILVSSVPKESSAASYSLR 360
           V FLLESLGLSN DD+LPIY+GDD TDEDAF+VL++ N G+GILVSS+PKES+ A YSLR
Sbjct: 301 VEFLLESLGLSNKDDLLPIYIGDDTTDEDAFKVLRDGNRGFGILVSSIPKESN-AFYSLR 360

Query: 361 EPSEVMEFLKSLVTWRALEE 374
           +PSEV +FLK+LV W  LE+
Sbjct: 361 DPSEVKKFLKTLVKWAKLEK 363

BLAST of Carg08012 vs. TAIR 10
Match: AT4G22590.1 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 448.4 bits (1152), Expect = 5.9e-126
Identity = 236/385 (61.30%), Postives = 287/385 (74.55%), Query Frame = 0

Query: 1   MDLKPNHNSPILSDP-APITKSRLGVHSNMLPYSAAPGVGFAPNLLLITRKRTGVLDDVR 60
           MDL  N  +P+LSDP  P++K+RLG        S+ P   F  N    +RK+   LDDVR
Sbjct: 1   MDLNINKTTPVLSDPTTPVSKTRLG--------SSFPSGRFMMN----SRKKIPKLDDVR 60

Query: 61  ASIWLDAMKSSSPPPNRIAKDVVNELPSFDPDLLYCSWMVKYPSALASFDQIVTQAKGKR 120
           ++ WLDAM SSSPP  R+ KD   E+   D D    +WM+KYPSA+ SF  I  QAK K+
Sbjct: 61  SNGWLDAMISSSPPRKRLVKDFNIEIAPED-DFSQRAWMLKYPSAITSFAHIAAQAKNKK 120

Query: 121 IALFLDYDGTLSPIVDNPDCAFMSDTMRVAVKEAAKHFPTAIISGRSRDKVYEFIGLNEL 180
           IA+FLDYDGTLSPIVD+PD A MSD MR AVK+ AK+FPTAIISGRSRDKVY+ +GL EL
Sbjct: 121 IAVFLDYDGTLSPIVDDPDRAIMSDAMRAAVKDVAKYFPTAIISGRSRDKVYQLVGLTEL 180

Query: 181 SYAGSHGMDIMVSDK-----------HSTNDQGKEV-MFQPASEFLPLIDEVYESLIEIA 240
            YAGSHGMDIM                +T+ QG+EV +FQPA EF+P+I+EVY +L+EI 
Sbjct: 181 YYAGSHGMDIMTPVNPNGSPEDPNCIKTTDQQGEEVNLFQPAKEFIPVIEEVYNNLVEIT 240

Query: 241 KGIAGAKVENNKFCVSVHYRNVDDKDWNALANRVYDLLENYPRLRVSHGRKVLEVRPVIS 300
           K I GAKVEN+KFC SVHYRNVD+KDW  +A RV+D L+ YPRLR++HGRKVLEVRPVI 
Sbjct: 241 KCIKGAKVENHKFCTSVHYRNVDEKDWPLVAQRVHDHLKRYPRLRITHGRKVLEVRPVIE 300

Query: 301 WDKGKAVTFLLESLGLSNCDDVLPIYVGDDRTDEDAFRVLKERNCGYGILVSSVPKESSA 360
           W+KGKAV FLLESLGLSN D+ LPI++GDD+TDEDAF+VL+E N G+GILVSSVPKES+ 
Sbjct: 301 WNKGKAVEFLLESLGLSNNDEFLPIFIGDDKTDEDAFKVLREGNRGFGILVSSVPKESN- 360

Query: 361 ASYSLREPSEVMEFLKSLVTWRALE 373
           A YSLR+PSEV +FLK+LV W  +E
Sbjct: 361 AFYSLRDPSEVKKFLKTLVKWGKME 371

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6600173.14.0e-217100.00Trehalose-phosphate phosphatase A, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022941645.11.5e-21699.73trehalose-phosphate phosphatase A-like [Cucurbita moschata] >XP_022941646.1 treh... [more]
XP_022966874.15.7e-21699.47trehalose-phosphate phosphatase A-like [Cucurbita maxima] >XP_022966947.1 trehal... [more]
XP_023525097.11.7e-21599.20trehalose-phosphate phosphatase A-like [Cucurbita pepo subsp. pepo] >XP_02352510... [more]
XP_038877832.15.2e-19390.79trehalose-phosphate phosphatase A [Benincasa hispida] >XP_038877836.1 trehalose-... [more]
Match NameE-valueIdentityDescription
O648963.9e-13563.90Trehalose-phosphate phosphatase A OS=Arabidopsis thaliana OX=3702 GN=TPPA PE=1 S... [more]
Q9SU393.7e-12561.58Probable trehalose-phosphate phosphatase F OS=Arabidopsis thaliana OX=3702 GN=TP... [more]
Q9SUW08.2e-12561.30Probable trehalose-phosphate phosphatase G OS=Arabidopsis thaliana OX=3702 GN=TP... [more]
Q9FWQ23.5e-11558.44Probable trehalose-phosphate phosphatase 2 OS=Oryza sativa subsp. japonica OX=39... [more]
Q75WV31.0e-11457.64Probable trehalose-phosphate phosphatase 1 OS=Oryza sativa subsp. japonica OX=39... [more]
Match NameE-valueIdentityDescription
A0A6J1FLP07.3e-21799.73Trehalose 6-phosphate phosphatase OS=Cucurbita moschata OX=3662 GN=LOC111446945 ... [more]
A0A6J1HQX92.8e-21699.47Trehalose 6-phosphate phosphatase OS=Cucurbita maxima OX=3661 GN=LOC111466421 PE... [more]
A0A6J1J8A81.3e-19290.51Trehalose 6-phosphate phosphatase OS=Cucurbita maxima OX=3661 GN=LOC111483480 PE... [more]
A0A6J1E8M34.8e-19290.24Trehalose 6-phosphate phosphatase OS=Cucurbita moschata OX=3662 GN=LOC111430866 ... [more]
A0A1S3AYK79.3e-18888.35Trehalose 6-phosphate phosphatase OS=Cucumis melo OX=3656 GN=LOC103483982 PE=3 S... [more]
Match NameE-valueIdentityDescription
AT5G51460.21.1e-13764.06Haloacid dehalogenase-like hydrolase (HAD) superfamily protein [more]
AT5G51460.32.8e-13663.90Haloacid dehalogenase-like hydrolase (HAD) superfamily protein [more]
AT5G51460.12.8e-13663.90Haloacid dehalogenase-like hydrolase (HAD) superfamily protein [more]
AT4G12430.12.6e-12661.58Haloacid dehalogenase-like hydrolase (HAD) superfamily protein [more]
AT4G22590.15.9e-12661.30Haloacid dehalogenase-like hydrolase (HAD) superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003337Trehalose-phosphataseTIGRFAMTIGR00685TIGR00685coord: 117..366
e-value: 1.4E-47
score: 160.6
IPR003337Trehalose-phosphatasePFAMPF02358Trehalose_PPasecoord: 123..356
e-value: 7.6E-73
score: 244.5
IPR006379HAD-superfamily hydrolase, subfamily IIBTIGRFAMTIGR01484TIGR01484coord: 122..329
e-value: 3.8E-20
score: 70.8
IPR023214HAD superfamilyGENE3D3.40.50.1000coord: 120..205
e-value: 1.6E-22
score: 82.1
coord: 208..374
e-value: 8.8E-22
score: 79.7
IPR044651Trehalose 6-phosphate OTSB-likePANTHERPTHR43768TREHALOSE 6-PHOSPHATE PHOSPHATASEcoord: 1..370
NoneNo IPR availablePANTHERPTHR43768:SF27TREHALOSE-PHOSPHATE PHOSPHATASE Acoord: 1..370
NoneNo IPR availableCDDcd01627HAD_TPPcoord: 121..360
e-value: 2.62953E-57
score: 184.8
IPR036412HAD-like superfamilySUPERFAMILY56784HAD-likecoord: 121..367

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg08012-RACarg08012-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016311 dephosphorylation
biological_process GO:0005992 trehalose biosynthetic process
molecular_function GO:0003677 DNA binding
molecular_function GO:0004805 trehalose-phosphatase activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0003824 catalytic activity