Carg07912 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg07912
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionNon-specific serine/threonine protein kinase
LocationCarg_Chr07: 1672756 .. 1675550 (+)
RNA-Seq ExpressionCarg07912
SyntenyCarg07912
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GTATCTTTAAAATTTGTCTATAATCTTTCAATTCTTTGTCCAATAAACTTTAATTTTTTTCAATAAATTTCTTAATTATGTGTTGTTCTATTCAAAGATACAAATTTCATCACGGAATTATATATATTTTGTTGAAATCTAAGAATTTTGAGAAGTTTACTCGATTAAAATGTTTGTGTTAATTTCAGACGAAGAAGGAAGAGAAATCGGAGAAGGGTTTGGGTTTGGGTTTAGGGGAAATGCCGAAGATTGGAGATTCCAAGATTGTATACAGTTGAAGAAGCTTCGAATCTCTCAATCGCTGCCTGTTCCTTCACGTTCTTCCAAGATTTCTTCTCTATTTGAAGATCTCGCTATAAATCATCTCCTATTTGTTTCAATTTTCGTCGAGACATTTCTACTGGCTCTCGAATGAAGTGCTGTTCTTCCTTTCGCTATTTATGGATACTTCTATTGATACTGTTACGATTTTGGAGCTATCTTGGCGCGTCGAGGAATTTTGAGAATTCAGGATTCTCTGGATTGAGCCGGTATGGGGGTTTGGATGATTCTGAAGCCGTAGCTCGAATTGGCGGCAGATCCCTCTTGTCCGTTCCAACGTGAGTCTTCAATTCATTTCTCTCGTTTCCTTATTAATATGCAACGAAGATTCTTTAATCTCTTTGATCAACCTTGATCGAGGATATTTCTTGTAACCTCAACTCATCTACTTGCTCTTTGGACAAATTAATCGTTTATAGAACGAAATACTTATCCACCATTTGATGCTTCCATCTCTTCACTATCAATAAAAATAGTCTTTCAAATCCGTAAGAACTAATCCAAATCAATACCATTCTTCTGCTATTAGTTTTGTATTTTGTAAATCAATGTAATGAGTGGTACTGTCAATATATACTATCGTAGGAAAGGTAGTACTGCTCTTATAGCTGCTTTGGATGGGGCAATTCATTTGGTGGACAGTAACTCTATGAAGATAATTTGGTCCTTCAGTTCTGGACCGCCAATCTATTCGTCACATCAAGCTCATGTTCATCATGAGCAGAATCAAGAAAATGCTTCAGGTTTGAGCAGATCCTTCTTTTTTTATTGTGGGGATGACTGGGAGCTCTACATGCACACAGAGTATGGTGAAACGGTAAAATTTAGTGAATGAATAACACCATCATGTGTTAATGTTTTCTTTCGAAATTTGTTTCAGTATTGATTCTGCAATCGCTGTTTTGATTGTCTTTCCTTTTTTTCATTTTTGATATGAATATATATTTTCCTCTTGGGATTCCTTGACATTTCAGAAACTACCAAAAACGATTGATGAGGTTGTTAGAAGTTCACCATATATATTTGAGGATGGTACGGTGATGACTGGTTCTACGAAAACCACAGTTTTTGAGGTTGATCATGTGACTGGAGAGCTTATTCGTAGTCACATACCTGATCTATTGTCATCTGAACTAAGCAATGAAGATCTTGGTAGTTCTAAAGCGAAAAATAATCTAGACGATAAGGATTTGATGCAACCTGGCGTGATGAACCCCCCTGTTGAGCCACGATTGTACATCACAAGAACAGATTATTCTCTGAAATCATCCTTTTCGGGTTCTAAAGAAGTTTCCTGGAGTTTGAGTGTTGCTGAAATCGGAGCTACCTTACTCTGTCCAGAGGTTGAGAATCCAGTTGAGGGCATTCCTTGGAATCTTCAAAATAATAGTAGTTATGAAGTTGATTATGCTGTGCCATTGTCTTGCCAATCAAAAGTACTTGTCTTTCGTGAACGCCATGTTTTATCAGGAAAGTTGGGGTATAAAAGATTATCGAAGGCTCATAATGTTGATAATATGTCTTTGGTGTCTGCTTCAGGTTCGATCCTTCCTTCCCAGTTAAAGATTGATAAACATTTAAACAGTCATCCTGAAAGATTGATGCTTCCTGAGCCTGTATCAAACAATATCTCTTCTTTGCCACCCAATTCAAGTAGCGAAGCAGTAGTCCCAATGCCTTTGATGAAGGTTAATGAATCAAGTATCTTCAAGGGGTTTAATAAAATGGAGACTCTTGCTGGACCTATTGGATTGCTTTTTGTAGTTTTAATAACCATGCTAGTGGGATTGATGAAGTATGGCAGGACATTGGCTGTAAAGGTAAAACAGCGTTTCTTCAATGAGAAGTTGTCTAGTACTTATAATGCAAGAATTATCTCTTCGAAGAAAAACAAACCTCGAAAATCAAAAAGAAGTGGAAATTCTGAGAAAATGGATGCATCTATTTCATCAGAAATTGAAAATTCGCTTATGCAAAGTGAGAATAACTGGTTTCAGGACAATAATCTTATTGGTGGACGTCGGATTGGTAAACTAATTGTAACCAACAAAGAGATAGCCAAGGGTAGTAATGGCACCGTTATTCTTGAGGGAATCTATGAAGGCCGAGTAGTTGCTGTGAAGCGCCTTGTCAAGGCTCACCATGATGTTGCCTCTAAGGAAGTTCAAAATTTAATGGCTTCTGATTTTCACCAAAATATCGTTCGATGGTATGGCACGGAGAGTGATCAAGATTTTGTCTATATTTCATTGGAACGGTGCACTTGCAGCCTCGATGATTTGATTCAGATTTGTTCTGATCAGTCCTTGAACTCTATGCTTGGCTTGGATCAAGATACTGTACCCATGATTGATTCAGTCAAAGAGGTTATACCAGATTTGAAATTGTGGAAAGAAAATGGCCATCCATCTACAGTTGTTCTAAAACTCATGAGGTTCTCTATATTTATTATCCAACACAATAATGATCTTTAG

mRNA sequence

GTATCTTTAAAATTTGTCTATAATCTTTCAATTCTTTGTCCAATAAACTTTAATTTTTTTCAATAAATTTCTTAATTATGTGTTGTTCTATTCAAAGATACAAATTTCATCACGGAATTATATATATTTTGTTGAAATCTAAGAATTTTGAGAAGTTTACTCGATTAAAATGTTTGTGTTAATTTCAGACGAAGAAGGAAGAGAAATCGGAGAAGGGTTTGGGTTTGGGTTTAGGGGAAATGCCGAAGATTGGAGATTCCAAGATTGTATACAGTTGAAGAAGCTTCGAATCTCTCAATCGCTGCCTGTTCCTTCACGTTCTTCCAAGATTTCTTCTCTATTTGAAGATCTCGCTATAAATCATCTCCTATTTGTTTCAATTTTCGTCGAGACATTTCTACTGGCTCTCGAATGAAGTGCTGTTCTTCCTTTCGCTATTTATGGATACTTCTATTGATACTGTTACGATTTTGGAGCTATCTTGGCGCGTCGAGGAATTTTGAGAATTCAGGATTCTCTGGATTGAGCCGGTATGGGGGTTTGGATGATTCTGAAGCCGTAGCTCGAATTGGCGGCAGATCCCTCTTGTCCGTTCCAACGAAAGGTAGTACTGCTCTTATAGCTGCTTTGGATGGGGCAATTCATTTGGTGGACAGTAACTCTATGAAGATAATTTGGTCCTTCAGTTCTGGACCGCCAATCTATTCGTCACATCAAGCTCATGTTCATCATGAGCAGAATCAAGAAAATGCTTCAGGTTTGAGCAGATCCTTCTTTTTTTATTGTGGGGATGACTGGGAGCTCTACATGCACACAGAGTATGGTGAAACGAAACTACCAAAAACGATTGATGAGGTTGTTAGAAGTTCACCATATATATTTGAGGATGGTACGGTGATGACTGGTTCTACGAAAACCACAGTTTTTGAGGTTGATCATGTGACTGGAGAGCTTATTCGTAGTCACATACCTGATCTATTGTCATCTGAACTAAGCAATGAAGATCTTGGTAGTTCTAAAGCGAAAAATAATCTAGACGATAAGGATTTGATGCAACCTGGCGTGATGAACCCCCCTGTTGAGCCACGATTGTACATCACAAGAACAGATTATTCTCTGAAATCATCCTTTTCGGGTTCTAAAGAAGTTTCCTGGAGTTTGAGTGTTGCTGAAATCGGAGCTACCTTACTCTGTCCAGAGGTTGAGAATCCAGTTGAGGGCATTCCTTGGAATCTTCAAAATAATAGTAGTTATGAAGTTGATTATGCTGTGCCATTGTCTTGCCAATCAAAAGTACTTGTCTTTCGTGAACGCCATGTTTTATCAGGAAAGTTGGGGTATAAAAGATTATCGAAGGCTCATAATGTTGATAATATGTCTTTGGTGTCTGCTTCAGGTTCGATCCTTCCTTCCCAGTTAAAGATTGATAAACATTTAAACAGTCATCCTGAAAGATTGATGCTTCCTGAGCCTGTATCAAACAATATCTCTTCTTTGCCACCCAATTCAAGTAGCGAAGCAGTAGTCCCAATGCCTTTGATGAAGGTTAATGAATCAAGTATCTTCAAGGGGTTTAATAAAATGGAGACTCTTGCTGGACCTATTGGATTGCTTTTTGTAGTTTTAATAACCATGCTAGTGGGATTGATGAAGTATGGCAGGACATTGGCTGTAAAGGTAAAACAGCGTTTCTTCAATGAGAAGTTGTCTAGTACTTATAATGCAAGAATTATCTCTTCGAAGAAAAACAAACCTCGAAAATCAAAAAGAAGTGGAAATTCTGAGAAAATGGATGCATCTATTTCATCAGAAATTGAAAATTCGCTTATGCAAAGTGAGAATAACTGGTTTCAGGACAATAATCTTATTGGTGGACGTCGGATTGGTAAACTAATTGTAACCAACAAAGAGATAGCCAAGGGTAGTAATGGCACCGTTATTCTTGAGGGAATCTATGAAGGCCGAGTAGTTGCTGTGAAGCGCCTTGTCAAGGCTCACCATGATGTTGCCTCTAAGGAAGTTCAAAATTTAATGGCTTCTGATTTTCACCAAAATATCGTTCGATGGTATGGCACGGAGAGTGATCAAGATTTTGTCTATATTTCATTGGAACGGTGCACTTGCAGCCTCGATGATTTGATTCAGATTTGTTCTGATCAGTCCTTGAACTCTATGCTTGGCTTGGATCAAGATACTGTACCCATGATTGATTCAGTCAAAGAGGTTATACCAGATTTGAAATTGTGGAAAGAAAATGGCCATCCATCTACAGTTGTTCTAAAACTCATGAGGTTCTCTATATTTATTATCCAACACAATAATGATCTTTAG

Coding sequence (CDS)

ATGAAGTGCTGTTCTTCCTTTCGCTATTTATGGATACTTCTATTGATACTGTTACGATTTTGGAGCTATCTTGGCGCGTCGAGGAATTTTGAGAATTCAGGATTCTCTGGATTGAGCCGGTATGGGGGTTTGGATGATTCTGAAGCCGTAGCTCGAATTGGCGGCAGATCCCTCTTGTCCGTTCCAACGAAAGGTAGTACTGCTCTTATAGCTGCTTTGGATGGGGCAATTCATTTGGTGGACAGTAACTCTATGAAGATAATTTGGTCCTTCAGTTCTGGACCGCCAATCTATTCGTCACATCAAGCTCATGTTCATCATGAGCAGAATCAAGAAAATGCTTCAGGTTTGAGCAGATCCTTCTTTTTTTATTGTGGGGATGACTGGGAGCTCTACATGCACACAGAGTATGGTGAAACGAAACTACCAAAAACGATTGATGAGGTTGTTAGAAGTTCACCATATATATTTGAGGATGGTACGGTGATGACTGGTTCTACGAAAACCACAGTTTTTGAGGTTGATCATGTGACTGGAGAGCTTATTCGTAGTCACATACCTGATCTATTGTCATCTGAACTAAGCAATGAAGATCTTGGTAGTTCTAAAGCGAAAAATAATCTAGACGATAAGGATTTGATGCAACCTGGCGTGATGAACCCCCCTGTTGAGCCACGATTGTACATCACAAGAACAGATTATTCTCTGAAATCATCCTTTTCGGGTTCTAAAGAAGTTTCCTGGAGTTTGAGTGTTGCTGAAATCGGAGCTACCTTACTCTGTCCAGAGGTTGAGAATCCAGTTGAGGGCATTCCTTGGAATCTTCAAAATAATAGTAGTTATGAAGTTGATTATGCTGTGCCATTGTCTTGCCAATCAAAAGTACTTGTCTTTCGTGAACGCCATGTTTTATCAGGAAAGTTGGGGTATAAAAGATTATCGAAGGCTCATAATGTTGATAATATGTCTTTGGTGTCTGCTTCAGGTTCGATCCTTCCTTCCCAGTTAAAGATTGATAAACATTTAAACAGTCATCCTGAAAGATTGATGCTTCCTGAGCCTGTATCAAACAATATCTCTTCTTTGCCACCCAATTCAAGTAGCGAAGCAGTAGTCCCAATGCCTTTGATGAAGGTTAATGAATCAAGTATCTTCAAGGGGTTTAATAAAATGGAGACTCTTGCTGGACCTATTGGATTGCTTTTTGTAGTTTTAATAACCATGCTAGTGGGATTGATGAAGTATGGCAGGACATTGGCTGTAAAGGTAAAACAGCGTTTCTTCAATGAGAAGTTGTCTAGTACTTATAATGCAAGAATTATCTCTTCGAAGAAAAACAAACCTCGAAAATCAAAAAGAAGTGGAAATTCTGAGAAAATGGATGCATCTATTTCATCAGAAATTGAAAATTCGCTTATGCAAAGTGAGAATAACTGGTTTCAGGACAATAATCTTATTGGTGGACGTCGGATTGGTAAACTAATTGTAACCAACAAAGAGATAGCCAAGGGTAGTAATGGCACCGTTATTCTTGAGGGAATCTATGAAGGCCGAGTAGTTGCTGTGAAGCGCCTTGTCAAGGCTCACCATGATGTTGCCTCTAAGGAAGTTCAAAATTTAATGGCTTCTGATTTTCACCAAAATATCGTTCGATGGTATGGCACGGAGAGTGATCAAGATTTTGTCTATATTTCATTGGAACGGTGCACTTGCAGCCTCGATGATTTGATTCAGATTTGTTCTGATCAGTCCTTGAACTCTATGCTTGGCTTGGATCAAGATACTGTACCCATGATTGATTCAGTCAAAGAGGTTATACCAGATTTGAAATTGTGGAAAGAAAATGGCCATCCATCTACAGTTGTTCTAAAACTCATGAGGTTCTCTATATTTATTATCCAACACAATAATGATCTTTAG

Protein sequence

MKCCSSFRYLWILLLILLRFWSYLGASRNFENSGFSGLSRYGGLDDSEAVARIGGRSLLSVPTKGSTALIAALDGAIHLVDSNSMKIIWSFSSGPPIYSSHQAHVHHEQNQENASGLSRSFFFYCGDDWELYMHTEYGETKLPKTIDEVVRSSPYIFEDGTVMTGSTKTTVFEVDHVTGELIRSHIPDLLSSELSNEDLGSSKAKNNLDDKDLMQPGVMNPPVEPRLYITRTDYSLKSSFSGSKEVSWSLSVAEIGATLLCPEVENPVEGIPWNLQNNSSYEVDYAVPLSCQSKVLVFRERHVLSGKLGYKRLSKAHNVDNMSLVSASGSILPSQLKIDKHLNSHPERLMLPEPVSNNISSLPPNSSSEAVVPMPLMKVNESSIFKGFNKMETLAGPIGLLFVVLITMLVGLMKYGRTLAVKVKQRFFNEKLSSTYNARIISSKKNKPRKSKRSGNSEKMDASISSEIENSLMQSENNWFQDNNLIGGRRIGKLIVTNKEIAKGSNGTVILEGIYEGRVVAVKRLVKAHHDVASKEVQNLMASDFHQNIVRWYGTESDQDFVYISLERCTCSLDDLIQICSDQSLNSMLGLDQDTVPMIDSVKEVIPDLKLWKENGHPSTVVLKLMRFSIFIIQHNNDL
Homology
[Close]
BLAST of Carg07912 vs. NCBI nr
Match: KAG7026699.1 (Serine/threonine-protein kinase/endoribonuclease IRE1b [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1253.8 bits (3243), Expect = 0.0e+00
Identity = 639/639 (100.00%), Postives = 639/639 (100.00%), Query Frame = 0 

Query: 1   MKCCSSFRYLWILLLILLRFWSYLGASRNFENSGFSGLSRYGGLDDSEAVARIGGRSLLS 60
           MKCCSSFRYLWILLLILLRFWSYLGASRNFENSGFSGLSRYGGLDDSEAVARIGGRSLLS
Sbjct: 1   MKCCSSFRYLWILLLILLRFWSYLGASRNFENSGFSGLSRYGGLDDSEAVARIGGRSLLS 60

Query: 61  VPTKGSTALIAALDGAIHLVDSNSMKIIWSFSSGPPIYSSHQAHVHHEQNQENASGLSRS 120
           VPTKGSTALIAALDGAIHLVDSNSMKIIWSFSSGPPIYSSHQAHVHHEQNQENASGLSRS
Sbjct: 61  VPTKGSTALIAALDGAIHLVDSNSMKIIWSFSSGPPIYSSHQAHVHHEQNQENASGLSRS 120

Query: 121 FFFYCGDDWELYMHTEYGETKLPKTIDEVVRSSPYIFEDGTVMTGSTKTTVFEVDHVTGE 180
           FFFYCGDDWELYMHTEYGETKLPKTIDEVVRSSPYIFEDGTVMTGSTKTTVFEVDHVTGE
Sbjct: 121 FFFYCGDDWELYMHTEYGETKLPKTIDEVVRSSPYIFEDGTVMTGSTKTTVFEVDHVTGE 180

Query: 181 LIRSHIPDLLSSELSNEDLGSSKAKNNLDDKDLMQPGVMNPPVEPRLYITRTDYSLKSSF 240
           LIRSHIPDLLSSELSNEDLGSSKAKNNLDDKDLMQPGVMNPPVEPRLYITRTDYSLKSSF
Sbjct: 181 LIRSHIPDLLSSELSNEDLGSSKAKNNLDDKDLMQPGVMNPPVEPRLYITRTDYSLKSSF 240

Query: 241 SGSKEVSWSLSVAEIGATLLCPEVENPVEGIPWNLQNNSSYEVDYAVPLSCQSKVLVFRE 300
           SGSKEVSWSLSVAEIGATLLCPEVENPVEGIPWNLQNNSSYEVDYAVPLSCQSKVLVFRE
Sbjct: 241 SGSKEVSWSLSVAEIGATLLCPEVENPVEGIPWNLQNNSSYEVDYAVPLSCQSKVLVFRE 300

Query: 301 RHVLSGKLGYKRLSKAHNVDNMSLVSASGSILPSQLKIDKHLNSHPERLMLPEPVSNNIS 360
           RHVLSGKLGYKRLSKAHNVDNMSLVSASGSILPSQLKIDKHLNSHPERLMLPEPVSNNIS
Sbjct: 301 RHVLSGKLGYKRLSKAHNVDNMSLVSASGSILPSQLKIDKHLNSHPERLMLPEPVSNNIS 360

Query: 361 SLPPNSSSEAVVPMPLMKVNESSIFKGFNKMETLAGPIGLLFVVLITMLVGLMKYGRTLA 420
           SLPPNSSSEAVVPMPLMKVNESSIFKGFNKMETLAGPIGLLFVVLITMLVGLMKYGRTLA
Sbjct: 361 SLPPNSSSEAVVPMPLMKVNESSIFKGFNKMETLAGPIGLLFVVLITMLVGLMKYGRTLA 420

Query: 421 VKVKQRFFNEKLSSTYNARIISSKKNKPRKSKRSGNSEKMDASISSEIENSLMQSENNWF 480
           VKVKQRFFNEKLSSTYNARIISSKKNKPRKSKRSGNSEKMDASISSEIENSLMQSENNWF
Sbjct: 421 VKVKQRFFNEKLSSTYNARIISSKKNKPRKSKRSGNSEKMDASISSEIENSLMQSENNWF 480

Query: 481 QDNNLIGGRRIGKLIVTNKEIAKGSNGTVILEGIYEGRVVAVKRLVKAHHDVASKEVQNL 540
           QDNNLIGGRRIGKLIVTNKEIAKGSNGTVILEGIYEGRVVAVKRLVKAHHDVASKEVQNL
Sbjct: 481 QDNNLIGGRRIGKLIVTNKEIAKGSNGTVILEGIYEGRVVAVKRLVKAHHDVASKEVQNL 540

Query: 541 MASDFHQNIVRWYGTESDQDFVYISLERCTCSLDDLIQICSDQSLNSMLGLDQDTVPMID 600
           MASDFHQNIVRWYGTESDQDFVYISLERCTCSLDDLIQICSDQSLNSMLGLDQDTVPMID
Sbjct: 541 MASDFHQNIVRWYGTESDQDFVYISLERCTCSLDDLIQICSDQSLNSMLGLDQDTVPMID 600

Query: 601 SVKEVIPDLKLWKENGHPSTVVLKLMRFSIFIIQHNNDL 640
           SVKEVIPDLKLWKENGHPSTVVLKLMRFSIFIIQHNNDL
Sbjct: 601 SVKEVIPDLKLWKENGHPSTVVLKLMRFSIFIIQHNNDL 639

[Close]
BLAST of Carg07912 vs. NCBI nr
Match: XP_022926588.1 (serine/threonine-protein kinase/endoribonuclease IRE1a-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1231.5 bits (3185), Expect = 0.0e+00
Identity = 629/639 (98.44%), Postives = 633/639 (99.06%), Query Frame = 0 

Query: 1   MKCCSSFRYLWILLLILLRFWSYLGASRNFENSGFSGLSRYGGLDDSEAVARIGGRSLLS 60
           MKCCSSFRYLWILLLILLRFWSYLGASRNFENSGFSGLSRYGGLDDSEAVARIGGRSLLS
Sbjct: 1   MKCCSSFRYLWILLLILLRFWSYLGASRNFENSGFSGLSRYGGLDDSEAVARIGGRSLLS 60

Query: 61  VPTKGSTALIAALDGAIHLVDSNSMKIIWSFSSGPPIYSSHQAHVHHEQNQENASGLSRS 120
           VPTKGSTALIAALDGAIHLVDSNSMKIIWSFSSGPPIYSSHQAHVHHEQNQENASGLSRS
Sbjct: 61  VPTKGSTALIAALDGAIHLVDSNSMKIIWSFSSGPPIYSSHQAHVHHEQNQENASGLSRS 120

Query: 121 FFFYCGDDWELYMHTEYGETKLPKTIDEVVRSSPYIFEDGTVMTGSTKTTVFEVDHVTGE 180
           FFFYCGDDWELYMHTEYGETKLPKTIDEVVRSSPYIFEDGTVMTGSTKTTVFEVDHVTGE
Sbjct: 121 FFFYCGDDWELYMHTEYGETKLPKTIDEVVRSSPYIFEDGTVMTGSTKTTVFEVDHVTGE 180

Query: 181 LIRSHIPDLLSSELSNEDLGSSKAKNNLDDKDLMQPGVMNPPVEPRLYITRTDYSLKSSF 240
           LIRSHIPDLLSSELSNEDLGSSKAKNNLDDKDLMQPGVMNPPVEPRLYITRTDYSLKSSF
Sbjct: 181 LIRSHIPDLLSSELSNEDLGSSKAKNNLDDKDLMQPGVMNPPVEPRLYITRTDYSLKSSF 240

Query: 241 SGSKEVSWSLSVAEIGATLLCPEVENPVEGIPWNLQNNSSYEVDYAVPLSCQSKVLVFRE 300
           SGSKEVSWSLSVAEIGATLLCPEVENPVEGIPWNLQNNSSYEVDYAVPLSCQSKVLVFRE
Sbjct: 241 SGSKEVSWSLSVAEIGATLLCPEVENPVEGIPWNLQNNSSYEVDYAVPLSCQSKVLVFRE 300

Query: 301 RHVLSGKLGYKRLSKAHNVDNMSLVSASGSILPSQLKIDKHLNSHPERLMLPEPVSNNIS 360
           RHVLSGKLGYKRLSKAHNVDNMSLVSASGSILPSQLKIDKHLNSHPERLMLPEPVSNNIS
Sbjct: 301 RHVLSGKLGYKRLSKAHNVDNMSLVSASGSILPSQLKIDKHLNSHPERLMLPEPVSNNIS 360

Query: 361 SLPPNSSSEAVVPMPLMKVNESSIFKGFNKMETLAGPIGLLFVVLITMLVGLMKYGRTLA 420
           SLPPNSSSEAVVPMPLMKVNESSIFKGFNKMETLAGPIGLLFVVLITMLVGLMKYGRTLA
Sbjct: 361 SLPPNSSSEAVVPMPLMKVNESSIFKGFNKMETLAGPIGLLFVVLITMLVGLMKYGRTLA 420

Query: 421 VKVKQRFFNEKLSSTYNARIISSKKNKPRKSKRSGNSEKMDASISSEIENSLMQSENNWF 480
           VKVKQRFFNEKLSSTYNARIISSKKNKPRKSKRSGNSEKMDASISSEIENSLMQSENNWF
Sbjct: 421 VKVKQRFFNEKLSSTYNARIISSKKNKPRKSKRSGNSEKMDASISSEIENSLMQSENNWF 480

Query: 481 QDNNLIGGRRIGKLIVTNKEIAKGSNGTVILEGIYEGRVVAVKRLVKAHHDVASKEVQNL 540
           QDNNLIGGRRIGKLIVTNKEIAKGSNGTVILEGIYEGRVVAVKRLVKAHHDVASKEVQNL
Sbjct: 481 QDNNLIGGRRIGKLIVTNKEIAKGSNGTVILEGIYEGRVVAVKRLVKAHHDVASKEVQNL 540

Query: 541 MASDFHQNIVRWYGTESDQDFVYISLERCTCSLDDLIQICSDQSLNSMLGLDQDTVPMID 600
           MASDFHQNIVRWYGTESDQDFVYISLERCTCSLDDLIQICSDQSLNSMLGLDQDTVPMID
Sbjct: 541 MASDFHQNIVRWYGTESDQDFVYISLERCTCSLDDLIQICSDQSLNSMLGLDQDTVPMID 600

Query: 601 SVKEVIPDLKLWKENGHPSTVVLKLMRFSIFIIQHNNDL 640
           SVKEVIPDLKLWKENGHPSTVVLKLMR  +  + H ++L
Sbjct: 601 SVKEVIPDLKLWKENGHPSTVVLKLMRDMVAGLVHLHEL 639

[Close]
BLAST of Carg07912 vs. NCBI nr
Match: KAG6594729.1 (Serine/threonine-protein kinase/endoribonuclease IRE1b, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1211.4 bits (3133), Expect = 0.0e+00
Identity = 619/639 (96.87%), Postives = 627/639 (98.12%), Query Frame = 0 

Query: 1   MKCCSSFRYLWILLLILLRFWSYLGASRNFENSGFSGLSRYGGLDDSEAVARIGGRSLLS 60
           MKCCSSFRYLWILLLILLRFWSYLGASRNFENSGFSGLSRYGG DDSEAVARIGGRSLLS
Sbjct: 1   MKCCSSFRYLWILLLILLRFWSYLGASRNFENSGFSGLSRYGGFDDSEAVARIGGRSLLS 60

Query: 61  VPTKGSTALIAALDGAIHLVDSNSMKIIWSFSSGPPIYSSHQAHVHHEQNQENASGLSRS 120
           VPTKGSTALIAALDGAIHLVDSNSMKIIWSFSSGPPIYSSHQAHVHHEQNQENASGLSRS
Sbjct: 61  VPTKGSTALIAALDGAIHLVDSNSMKIIWSFSSGPPIYSSHQAHVHHEQNQENASGLSRS 120

Query: 121 FFFYCGDDWELYMHTEYGETKLPKTIDEVVRSSPYIFEDGTVMTGSTKTTVFEVDHVTGE 180
           FFFYCGDDWELYMHTEYGETKLPKTIDEVVRS+PYIFEDGTVMTGSTKTTVFEVDHVTGE
Sbjct: 121 FFFYCGDDWELYMHTEYGETKLPKTIDEVVRSTPYIFEDGTVMTGSTKTTVFEVDHVTGE 180

Query: 181 LIRSHIPDLLSSELSNEDLGSSKAKNNLDDKDLMQPGVMNPPVEPRLYITRTDYSLKSSF 240
           LIRSHIPDLLSSELSNEDLGSSKAKNNLDDKDLMQPGVMNPPVEPRLYITRTDYSLKSSF
Sbjct: 181 LIRSHIPDLLSSELSNEDLGSSKAKNNLDDKDLMQPGVMNPPVEPRLYITRTDYSLKSSF 240

Query: 241 SGSKEVSWSLSVAEIGATLLCPEVENPVEGIPWNLQNNSSYEVDYAVPLSCQSKVLVFRE 300
           SGSKEVSWSLSVAEIGATLLCPEVENPVEGIPWNLQNNSSYEVDYAVPLSCQSKVLVFRE
Sbjct: 241 SGSKEVSWSLSVAEIGATLLCPEVENPVEGIPWNLQNNSSYEVDYAVPLSCQSKVLVFRE 300

Query: 301 RHVLSGKLGYKRLSKAHNVDNMSLVSASGSILPSQLKIDKHLNSHPERLMLPEPVSNNIS 360
           RHVLSGKLGYKRLSKAHNVDNMSLVSASGSILPSQLKIDKHLN HPERLMLPEPVSN IS
Sbjct: 301 RHVLSGKLGYKRLSKAHNVDNMSLVSASGSILPSQLKIDKHLNIHPERLMLPEPVSNIIS 360

Query: 361 SLPPNSSSEAVVPMPLMKVNESSIFKGFNKMETLAGPIGLLFVVLITMLVGLMKYGRTLA 420
           SLPPNSSSEAVVPMPLMKVNESSIFKGFNKMETLAGPIGLLFVVLITMLVGLMKYGRTLA
Sbjct: 361 SLPPNSSSEAVVPMPLMKVNESSIFKGFNKMETLAGPIGLLFVVLITMLVGLMKYGRTLA 420

Query: 421 VKVKQRFFNEKLSSTYNARIISSKKNKPRKSKRSGNSEKMDASISSEIENSLMQSENNWF 480
           VKVKQRFFNEK SST NARI+SSKKNKPRKSKRSGNSEKMDASISSEIENSL+QSE+NWF
Sbjct: 421 VKVKQRFFNEKSSSTSNARIVSSKKNKPRKSKRSGNSEKMDASISSEIENSLIQSEDNWF 480

Query: 481 QDNNLIGGRRIGKLIVTNKEIAKGSNGTVILEGIYEGRVVAVKRLVKAHHDVASKEVQNL 540
           QDNNLIGGRRIGKL VTNKEIAKGSNGTVILEGIYEGRVVAVKRLVKAHHDVASKEVQNL
Sbjct: 481 QDNNLIGGRRIGKLTVTNKEIAKGSNGTVILEGIYEGRVVAVKRLVKAHHDVASKEVQNL 540

Query: 541 MASDFHQNIVRWYGTESDQDFVYISLERCTCSLDDLIQICSDQSLNSMLGLDQDTVPMID 600
           MASDFHQNIVRWYGTESDQDFVYISLERCTCSLDDLIQICSDQSLNSMLGLDQDTVPMID
Sbjct: 541 MASDFHQNIVRWYGTESDQDFVYISLERCTCSLDDLIQICSDQSLNSMLGLDQDTVPMID 600

Query: 601 SVKEVIPDLKLWKENGHPSTVVLKLMRFSIFIIQHNNDL 640
           SVKEVIPDLKLWKENGHPSTVVLKLMR  +  + H ++L
Sbjct: 601 SVKEVIPDLKLWKENGHPSTVVLKLMRDMVAGLVHLHEL 639

[Close]
BLAST of Carg07912 vs. NCBI nr
Match: XP_023518030.1 (serine/threonine-protein kinase/endoribonuclease IRE1a-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1198.7 bits (3100), Expect = 0.0e+00
Identity = 612/639 (95.77%), Postives = 622/639 (97.34%), Query Frame = 0 

Query: 1   MKCCSSFRYLWILLLILLRFWSYLGASRNFENSGFSGLSRYGGLDDSEAVARIGGRSLLS 60
           MKCCSSFRYLW+LLLILLRFWSYLGASRN ENSGFSGLSRYGGLDDSEAVARIGGRSLLS
Sbjct: 1   MKCCSSFRYLWMLLLILLRFWSYLGASRNSENSGFSGLSRYGGLDDSEAVARIGGRSLLS 60

Query: 61  VPTKGSTALIAALDGAIHLVDSNSMKIIWSFSSGPPIYSSHQAHVHHEQNQENASGLSRS 120
           VPTKGSTALIAALDGAIHLVDSNSMKIIWSFSSGPPIYSSHQAHVHHE NQENASGLSRS
Sbjct: 61  VPTKGSTALIAALDGAIHLVDSNSMKIIWSFSSGPPIYSSHQAHVHHEHNQENASGLSRS 120

Query: 121 FFFYCGDDWELYMHTEYGETKLPKTIDEVVRSSPYIFEDGTVMTGSTKTTVFEVDHVTGE 180
           FFFYCGDDWELYMHTEYGETKLPKTIDEVVRS+PYIFEDGTVMTGSTKTTVFEVDHVTGE
Sbjct: 121 FFFYCGDDWELYMHTEYGETKLPKTIDEVVRSTPYIFEDGTVMTGSTKTTVFEVDHVTGE 180

Query: 181 LIRSHIPDLLSSELSNEDLGSSKAKNNLDDKDLMQPGVMNPPVEPRLYITRTDYSLKSSF 240
           LIRSHIPDLLSSELSNEDLGS KA+NNLDDKDL+QPGVMNPPVEPRLYITRTDYSLKSSF
Sbjct: 181 LIRSHIPDLLSSELSNEDLGSYKARNNLDDKDLIQPGVMNPPVEPRLYITRTDYSLKSSF 240

Query: 241 SGSKEVSWSLSVAEIGATLLCPEVENPVEGIPWNLQNNSSYEVDYAVPLSCQSKVLVFRE 300
           SGSKEVSWSLSVAEIGATLLCPEVENP+EGIPWNLQNNSSYEVDYAVPLSCQSKVLVFRE
Sbjct: 241 SGSKEVSWSLSVAEIGATLLCPEVENPIEGIPWNLQNNSSYEVDYAVPLSCQSKVLVFRE 300

Query: 301 RHVLSGKLGYKRLSKAHNVDNMSLVSASGSILPSQLKIDKHLNSHPERLMLPEPVSNNIS 360
           RHVLSGK GYKRLSKAHNVDNMSLVSASGSILPSQLKIDKHLN HPERLMLPEPVSN IS
Sbjct: 301 RHVLSGKSGYKRLSKAHNVDNMSLVSASGSILPSQLKIDKHLNIHPERLMLPEPVSNTIS 360

Query: 361 SLPPNSSSEAVVPMPLMKVNESSIFKGFNKMETLAGPIGLLFVVLITMLVGLMKYGRTLA 420
           SLPPNSSSEAVVPMPLMKVNESSIFKG NKMETLAGPIGL FVVLITMLVGLMKYGRTLA
Sbjct: 361 SLPPNSSSEAVVPMPLMKVNESSIFKGCNKMETLAGPIGLFFVVLITMLVGLMKYGRTLA 420

Query: 421 VKVKQRFFNEKLSSTYNARIISSKKNKPRKSKRSGNSEKMDASISSEIENSLMQSENNWF 480
           VKVKQRFFNEK SST NARIISSKKNKPRKSKRSGNSEKMDASISSEIENSLMQSE NWF
Sbjct: 421 VKVKQRFFNEKSSSTSNARIISSKKNKPRKSKRSGNSEKMDASISSEIENSLMQSEENWF 480

Query: 481 QDNNLIGGRRIGKLIVTNKEIAKGSNGTVILEGIYEGRVVAVKRLVKAHHDVASKEVQNL 540
           QDNNLIGGRRIGKLIVTNKEIAKGSNGTVILEGIYEGRVVAVKRLVKAHHDVASKEVQNL
Sbjct: 481 QDNNLIGGRRIGKLIVTNKEIAKGSNGTVILEGIYEGRVVAVKRLVKAHHDVASKEVQNL 540

Query: 541 MASDFHQNIVRWYGTESDQDFVYISLERCTCSLDDLIQICSDQSLNSMLGLDQDTVPMID 600
           MASDFHQNI+RWYGTESDQDFVYISLERCTCSLDDLIQICSDQSLNSMLGLDQDTVPMID
Sbjct: 541 MASDFHQNIIRWYGTESDQDFVYISLERCTCSLDDLIQICSDQSLNSMLGLDQDTVPMID 600

Query: 601 SVKEVIPDLKLWKENGHPSTVVLKLMRFSIFIIQHNNDL 640
           SVKEVIPDLKLWKENGHPSTVVLKLMR  +  + H ++L
Sbjct: 601 SVKEVIPDLKLWKENGHPSTVVLKLMRDMVAGLVHLHEL 639

[Close]
BLAST of Carg07912 vs. NCBI nr
Match: XP_023003027.1 (serine/threonine-protein kinase/endoribonuclease IRE1a-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1188.7 bits (3074), Expect = 0.0e+00
Identity = 609/639 (95.31%), Postives = 618/639 (96.71%), Query Frame = 0 

Query: 1   MKCCSSFRYLWILLLILLRFWSYLGASRNFENSGFSGLSRYGGLDDSEAVARIGGRSLLS 60
           MK CSSFRYLWILLLILLRFWSYLGASRN ENSGFSGLSRYGG DDSEAVARIGGRSLLS
Sbjct: 1   MKRCSSFRYLWILLLILLRFWSYLGASRNSENSGFSGLSRYGGFDDSEAVARIGGRSLLS 60

Query: 61  VPTKGSTALIAALDGAIHLVDSNSMKIIWSFSSGPPIYSSHQAHVHHEQNQENASGLSRS 120
           VPTKGSTALIAALDGAIHLVDSNSMKIIWSFSSGPPIYS HQAHVHHE NQENASGLSR 
Sbjct: 61  VPTKGSTALIAALDGAIHLVDSNSMKIIWSFSSGPPIYSPHQAHVHHEHNQENASGLSRP 120

Query: 121 FFFYCGDDWELYMHTEYGETKLPKTIDEVVRSSPYIFEDGTVMTGSTKTTVFEVDHVTGE 180
           FFFYCGDDWELYMHTEYGETKLPKTIDEVVRS+PYIFEDGTVMTGSTKTTVFEVDHVTGE
Sbjct: 121 FFFYCGDDWELYMHTEYGETKLPKTIDEVVRSTPYIFEDGTVMTGSTKTTVFEVDHVTGE 180

Query: 181 LIRSHIPDLLSSELSNEDLGSSKAKNNLDDKDLMQPGVMNPPVEPRLYITRTDYSLKSSF 240
           LIRSHIPDLLSSELSNEDLGSSKAK+NLDDKDL+QPGVMNPPVEPRLYITRTDYSLKSSF
Sbjct: 181 LIRSHIPDLLSSELSNEDLGSSKAKSNLDDKDLIQPGVMNPPVEPRLYITRTDYSLKSSF 240

Query: 241 SGSKEVSWSLSVAEIGATLLCPEVENPVEGIPWNLQNNSSYEVDYAVPLSCQSKVLVFRE 300
           SGSKEVSWSLSVAEIGATLLCPEVENPVEGIPWNLQNNSSYEVDYAVPLSCQSKVLVFRE
Sbjct: 241 SGSKEVSWSLSVAEIGATLLCPEVENPVEGIPWNLQNNSSYEVDYAVPLSCQSKVLVFRE 300

Query: 301 RHVLSGKLGYKRLSKAHNVDNMSLVSASGSILPSQLKIDKHLNSHPERLMLPEPVSNNIS 360
           RH LSGK GYKRLSKAHNVDNMSLVSASGSILPSQLKIDKHLN HPERLMLPEPVSN IS
Sbjct: 301 RHALSGKSGYKRLSKAHNVDNMSLVSASGSILPSQLKIDKHLNIHPERLMLPEPVSNTIS 360

Query: 361 SLPPNSSSEAVVPMPLMKVNESSIFKGFNKMETLAGPIGLLFVVLITMLVGLMKYGRTLA 420
           SLPPNSSSEAVVPMPLMKVNESSIFKG NKMETLAGPIGL FVVLI MLVGLMKYGRTLA
Sbjct: 361 SLPPNSSSEAVVPMPLMKVNESSIFKGCNKMETLAGPIGLFFVVLIIMLVGLMKYGRTLA 420

Query: 421 VKVKQRFFNEKLSSTYNARIISSKKNKPRKSKRSGNSEKMDASISSEIENSLMQSENNWF 480
           VKVKQRFFNEK SST NARIISSKKNKPRKSKRSGNSEKMDASISSEIENSLMQSE+NWF
Sbjct: 421 VKVKQRFFNEKSSSTSNARIISSKKNKPRKSKRSGNSEKMDASISSEIENSLMQSEDNWF 480

Query: 481 QDNNLIGGRRIGKLIVTNKEIAKGSNGTVILEGIYEGRVVAVKRLVKAHHDVASKEVQNL 540
           QDNNLIGGRRIGKLIVTNKEIAKGSNGTVILEGIYEGRVVAVKRLVKAHHDVASKEVQNL
Sbjct: 481 QDNNLIGGRRIGKLIVTNKEIAKGSNGTVILEGIYEGRVVAVKRLVKAHHDVASKEVQNL 540

Query: 541 MASDFHQNIVRWYGTESDQDFVYISLERCTCSLDDLIQICSDQSLNSMLGLDQDTVPMID 600
           MASDFHQNIVRWYGTESDQDFVYISLERCTCSLDDLIQICSDQS NSMLGLDQDTVPMID
Sbjct: 541 MASDFHQNIVRWYGTESDQDFVYISLERCTCSLDDLIQICSDQSFNSMLGLDQDTVPMID 600

Query: 601 SVKEVIPDLKLWKENGHPSTVVLKLMRFSIFIIQHNNDL 640
           SVKEVIPDLKLWKENGHPSTVVLKLMR  +  ++H ++L
Sbjct: 601 SVKEVIPDLKLWKENGHPSTVVLKLMRDMVAGLEHLHEL 639

[Close]
BLAST of Carg07912 vs. ExPASy Swiss-Prot
Match: Q93VJ2 (Serine/threonine-protein kinase/endoribonuclease IRE1b OS=Arabidopsis thaliana OX=3702 GN=IRE1B PE=2 SV=1)

HSP 1 Score: 252.3 bits (643), Expect = 1.5e-65
Identity = 205/594 (34.51%), Postives = 299/594 (50.34%), Query Frame = 0 

Query: 69  LIAALDGAIHLVDSNSMKIIWSFSSGPPIYSSHQA-HVHHEQNQENASGLSRSFFFYCGD 128
           L++ +DG+I LVD +S K+ W+F +  PIYSS+QA H H+  ++E +S L   F+  C  
Sbjct: 50  LVSTVDGSISLVDMSSQKLDWTFHTNEPIYSSYQAPHYHYTTDEERSSVLGDDFYMDCDK 109

Query: 129 DWELYMHTEYGETKLPKTID--EVVRSSPYIFEDGTVMTGSTKTTVFEVDHVTGELIRSH 188
           DW LY  +     ++ + +D  E + + PY   D  V+ G   T+VF +D  TG+L++ +
Sbjct: 110 DWRLYNSSVRKGKRVNEIVDASEFIGTLPYTSTDRIVL-GKKDTSVFLLDWKTGKLVKRY 169

Query: 189 IPDLLSSELSNEDLGSSKAKNNLDDKD---LMQPGVMNPPVEPRL-YITRTDYSLKSSFS 248
             D L    SN  + + K K  +  K+   L   G       P L YI R D+ ++   S
Sbjct: 170 RMDEL---YSNTVVENDKEKAIVLSKEAPLLFGSGFKKSEDFPELVYIERKDFKIQ-CIS 229

Query: 249 GSKEVSWSLSVAEIGATLLCPEVENPVEGIPWNLQNNSSYEVDYAVPLSCQSKVLVFRER 308
              +V WS+S A++ A L   E    + G+  ++  N         PLS  + V + + R
Sbjct: 230 KFGDVLWSVSYAKMEAKLQNHESVQFISGLSSSVGKNQ-------FPLSYTTSVPMVQLR 289

Query: 309 HVLSGKLGYKRLSKAHNVDNMSLVSASGSILPSQLKIDKHLNSHPERLMLPEPVSNNISS 368
           +V      Y+ L       + +L        P+QL I                   N  +
Sbjct: 290 NV-----KYETLFPRLGFLDEALYLPFQDRKPNQLAIG----------------DGNQLT 349

Query: 369 LPPNSSSEAVVPMPLMKV---NESSIFKGFNKMETLA----GPIGLLFVVLITML--VGL 428
           LP N  +E V+ +PL +      + I  G  K    A    G I L+F   +TML   GL
Sbjct: 350 LPGNKEAEEVLSLPLPETVISQITDIIDGSTKQAGFASKFSGLIVLIFGFCVTMLSVCGL 409

Query: 429 MKYGRTLAVKVKQRFFNEKLSSTYNARIISSKKNKPRKSKRSGNSEKMDASISSEIENSL 488
             Y    ++++K+ + +E         I + KK K +K+  +    K +    S      
Sbjct: 410 FFYRLRQSIRIKEPYVSE-------VPIATPKKKKSKKNGTTKAVHKKENGFISGGNKDP 469

Query: 489 MQSENNW-------FQDNNLIGGRRIGKLIVTNKEIAKGSNGTVILEGIYEGRVVAVKRL 548
              EN           +N+   G R+GKL V+NKEIAKGSNGTV+LEG YEGR+VAVKRL
Sbjct: 470 SHEENEKRLLTAFPGLNNSSAEGYRVGKLFVSNKEIAKGSNGTVVLEGSYEGRLVAVKRL 529

Query: 549 VKAHHDVASKEVQNLMASDFHQNIVRWYGTESDQDFVYISLERCTCSLDDLIQICSDQSL 608
           V++HHDVA KE+ NLMASD H NIVRWYG + D+ F+YISLE C CSL+DLI   S   L
Sbjct: 530 VQSHHDVAQKEILNLMASDKHSNIVRWYGVDQDEHFIYISLELCACSLNDLI-YASSALL 589

Query: 609 NSMLGLDQDTVPMIDSVKEVIPDLKLWKENGHPSTVVLKLMRFSIFIIQHNNDL 640
            S +         I+ + E    ++LWKENGHPS V+LKLMR  +  + H +D+
Sbjct: 590 ESPMASSSIHSIQINPIFENGKGVELWKENGHPSPVLLKLMRDIVAGLVHLHDI 602

[Close]
BLAST of Carg07912 vs. ExPASy Swiss-Prot
Match: Q9C5S2 (Serine/threonine-protein kinase/endoribonuclease IRE1a OS=Arabidopsis thaliana OX=3702 GN=IRE1A PE=1 SV=1)

HSP 1 Score: 223.8 bits (569), Expect = 5.6e-57
Identity = 209/660 (31.67%), Postives = 304/660 (46.06%), Query Frame = 0 

Query: 4   CSSFRYLWILLLILLRFWSYLGASRNFENSGFSGLSRYGGLDDSEAVARI----GGRSLL 63
           C   R+L+ LLL LL  W                +S  GG  D      +    G RS+L
Sbjct: 5   CPFLRHLFFLLL-LLSPWI---------------MSPCGGAADDVTYPIVPSSPGRRSIL 64

Query: 64  SV----PTKGSTALIAALDGAIHLVDSNSMKIIWSFSSGPPIYSSHQAHVHHEQNQENAS 123
            +    PT+ +T L+   DG + L         WSFS+G P++S +QA  ++  N ENA+
Sbjct: 65  QIRREPPTEPNTKLVVDRDGKVFLKQQPKETPYWSFSTGSPMHSLYQAPANN--NTENAT 124

Query: 124 GLSRSFFFYCGDDWELYMHTEYGETKLPK----TIDEVVRSSPYIFEDGTVMTGSTKTTV 183
            ++R            Y++     T +      T+ E  R  P + +DG V  GS  T+ 
Sbjct: 125 EITRPHIIV------EYLNNSKAATTVDGYHNWTVQEFFRQKPLVTDDG-VTLGSETTSA 184

Query: 184 FEVDHVTGELIRSHIPDLLSSELSNEDLGSSKAKNNLDDKDLMQPGVMNPPVEPRLYITR 243
           + VD  +G LI  +           +  G +K  N      L++P      V   L I R
Sbjct: 185 YLVDGRSGRLIHVY-----------KSTGDTKITN-----ALVKPASTEDFVNEPLLIRR 244

Query: 244 TDYSLKSSFSGSKEVSWSLSVAEIGATLLCPEVENPVEGIPWNLQNNSSYEVDYAVPLSC 303
           TD  L+     + ++ W+L+V+   A LLC  V N    +   LQ          +PL C
Sbjct: 245 TDSKLEHFSKTTGKLVWNLTVSHFRAALLCDPVFNSGYDLGPKLQTG------IYMPLLC 304

Query: 304 QSKVLVFRERHVLSGKLGYKRLSKAHNVDNMSLVSASGSILPSQLKIDKHLNSHPERLML 363
            S+                              +   G  +  ++  D+ +N      ML
Sbjct: 305 GSQ------------------------------IDVRGPEIVIRVLHDQPMNVK----ML 364

Query: 364 PEPVSNNISS----LPPNSSSEAVVPMPLMKVNESSIFKGFNKMETLAGPIGLLFVVLIT 423
           P P  N+  S    +P   + E+       K   + +F  ++ ++ LA P+ LL VV+  
Sbjct: 365 PSPSLNHFESENSIMPFGKARESRKLQEQHKQKYTYLFGQWSPVKLLA-PLVLLGVVVSV 424

Query: 424 MLVGLMKYGRTLAVKVKQRFFNEKLSSTYNARIISSKKNKPRKSKRSGNSEKMDASIS-- 483
            +      G  +++K                    SKK K RKS +  N + +       
Sbjct: 425 FIKKFSSRGSDVSLKAG-----------------PSKKKKNRKSAKDTNRQSVPRGQDQF 484

Query: 484 SEIENSLMQSENNWFQDNNLIGGRRIGKLIVTNKEIAKGSNGTVILEGIYEGRVVAVKRL 543
             IE   M    N FQ +    GR+IGKL +++KEIAKGSNGTV+ EGIYEGR VAVKRL
Sbjct: 485 ELIEGGQMLLGFNNFQ-SGATDGRKIGKLFLSSKEIAKGSNGTVVFEGIYEGRPVAVKRL 544

Query: 544 VKAHHDVASKEVQNLMASDFHQNIVRWYGTESDQDFVYISLERCTCSLDDLIQICSDQSL 603
           V++HH+VA KE+QNL+ASD H NI+RWYG E DQDFVY+SLERCTCSLDDLI+   + S+
Sbjct: 545 VRSHHEVAFKEIQNLIASDQHTNIIRWYGVEYDQDFVYLSLERCTCSLDDLIKSYLEFSM 564

Query: 604 NSMLGLDQDTVPM------IDSVKEVIPDLKLWKENGHPSTVVLKLMRFSIFIIQHNNDL 640
             +L  +  T  +      +DS++ VI     WK  GHPS ++LKLMR  +  I H ++L
Sbjct: 605 TKVLENNDSTEGVAAYKIQLDSLEGVIKGNNFWKVGGHPSPLMLKLMRDIVCGIVHLHEL 564

[Close]
BLAST of Carg07912 vs. ExPASy Swiss-Prot
Match: Q7XIT1 (Serine/threonine-protein kinase/endoribonuclease IRE1 OS=Oryza sativa subsp. japonica OX=39947 GN=IRE1 PE=1 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 4.3e-41
Identity = 173/584 (29.62%), Postives = 261/584 (44.69%), Query Frame = 0 

Query: 74  DGAIHLVDSNSMKIIWSFSSGPPIYSSHQAHVHHEQNQENASGLSRSFFFYCGDDWELYM 133
           +G I LVD  S    W FS+GPP+ S H      + N            +  G +    +
Sbjct: 105 NGTIQLVDLLSKLPRWQFSTGPPL-SKHITTSKPDLNY---------VIYLDGSETSDLI 164

Query: 134 HTEYGE-TKLPKTIDEVVRSSPYIFEDGTVMTGSTKTTVFEVDHVTGELIRSHIPDLLSS 193
               G   +LP  ++E +  +PYI  D  V  GS  +T F V+  +GE+I  H     S 
Sbjct: 165 EVHNGSGVRLPWKLEEFIAETPYI-RDSFVTIGSKVSTTFVVNADSGEIIYKH-----SL 224

Query: 194 ELSNEDLGSSKAKNNLDDKDLMQPGVMNPPVEPRLYITRTDYSLKSSFSGSKEVSWSLS- 253
            ++  ++G    +      D  + G         + + RTDYS+ +S  G    +W+ + 
Sbjct: 225 PVALNEVGGPLVEEIPSKLDAARSGTS----ANIIVVVRTDYSISASDLGEHLFNWTRTS 284

Query: 254 -----VAEIGATLLCPEVENPVEGIPWNLQNNSSYEVDYAVPLSCQSKVLVFRERHVLSG 313
                 A  G   +  +       IP          +   +P S     +V R       
Sbjct: 285 FTANYYARYGHQDMLAQSSCLRGNIP--CIRTEGPPIKLYLPDSSSDNAIVLRP------ 344

Query: 314 KLGYKRLSKAHNVDNMSLVSASGSILPSQLKIDKHLNSHPERLMLPEPVSNNISSLPPNS 373
                       V+ +S V A   +LP            P++L  P   SN       N 
Sbjct: 345 ------------VNEVSAVDALEPLLP------------PKKLPQPAGESNVALDSAQNQ 404

Query: 374 SSEAVVP--MPLMKVNESSIFKGFNKMETLAGPIGLLFVVLITMLVGLMKYGRTLAVKVK 433
           +++  +   +P      +S+ K F+        + LL +  +  L    KY R   ++  
Sbjct: 405 TADIALGHFVPADTELTNSVTK-FSYRWLFPTFLMLLIMACLVKLADASKYCRQFVIRFL 464

Query: 434 QRFF-NEKLSSTYNARIISSKKNKPRKSKRSGNSEKMDASISSEIENS--LMQSENNWFQ 493
           + F  +EKL         +SK+ K RK     NS ++ ++   E   +    ++++N   
Sbjct: 465 KPFMRDEKLMDPRGKSEGTSKRRKARKKDGLINSTQIFSASDKEGNGTGGSTEAQSNKAH 524

Query: 494 DN------NLIGGRRIGKLIVTNKEIAKGSNGTVILEGIYEGRVVAVKRLVKAHHDVASK 553
           D+      N + GR+IGKL V +KEI KGSNGTV+ EG Y GR VAVKRL+++H+D+ASK
Sbjct: 525 DSTNVELPNGLNGRQIGKLCVYSKEIGKGSNGTVVFEGSYGGREVAVKRLLRSHNDIASK 584

Query: 554 EVQNLMASDFHQNIVRWYGTESDQDFVYISLERCTCSLDDLIQICSDQSLNSMLGLDQDT 613
           E++NL+ASD   NIVR YG E D DFVYISLERC CSL DLIQ+ S              
Sbjct: 585 EIENLIASDQDPNIVRMYGFEQDNDFVYISLERCRCSLADLIQLHS-------------- 619

Query: 614 VPMIDSVKEVIPDLKLWKENGHPSTVVLKLMRFSIFIIQHNNDL 640
           VP   + K    D++LW+++G PS  +LKLMR  +  I H + L
Sbjct: 645 VPPFSNTKGT--DIELWRQDGLPSAQLLKLMRDVVAGIVHLHSL 619

[Close]
BLAST of Carg07912 vs. ExPASy Swiss-Prot
Match: Q9SF12 (Inactive serine/threonine-protein kinase/endoribonuclease IRE1-like OS=Arabidopsis thaliana OX=3702 GN=At3g11870 PE=3 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 6.9e-15
Identity = 82/262 (31.30%), Postives = 120/262 (45.80%), Query Frame = 0 

Query: 398 IGLLFVVLITMLVGLMKYGRTLAVKVKQRFFNEKLSSTYNARIISSKKNKPRKSKRSG-- 457
           +GLL VV+   L      G    V  K+R  N     + +    +  +   +KS  SG  
Sbjct: 9   VGLLVVVVCVFLRFSKDKGLDGIVNEKKRDKNSAPRVSASGEDGTKNEQVEKKSDPSGGL 68

Query: 458 -------NSEK------MDASISSEIENSLMQSENNWFQDNNLIGGRRIGKLIVTNKEIA 517
                  NSE        D +I+  +   L   E   + +N   G     +L+V+  E+ 
Sbjct: 69  GEENEKTNSESKVLSVPSDQNINKTLPVMLPSLELRKYDENETPGKVVNRRLLVSTNEMK 128

Query: 518 KGSNGTVILEGIYEGR-VVAVKRLVKAHHDVA--SKEVQNLMASDFHQNIVRWYGTESDQ 577
            G NG  + +G+Y  R  VAVK L  AH   A    E+ N    D H NI+R++G E DQ
Sbjct: 129 YGRNGYEVFQGVYGRRSSVAVKCLDLAHTTEAFIQNEIDNHCLCDDHSNIIRFHGLEQDQ 188

Query: 578 DFVYISLERCTCSLDDLIQICSDQSLNSMLGLDQDTVPMIDSVKEVIPDLKLWKENGH-- 637
            F YI LE   CSLDDLI++   ++       D   V  +D +++V+  +K WKE G   
Sbjct: 189 SFAYICLEPWKCSLDDLIKLSVRRTKR-----DTQAVAPVDDLEKVMKRIKFWKEKGKPL 248

Query: 638 PSTVVLKLMRFSIFIIQHNNDL 640
           P T +LKLMR  +  + H + L
Sbjct: 249 PLTPMLKLMRDVVCGLAHLHKL 265

[Close]
BLAST of Carg07912 vs. ExPASy Swiss-Prot
Match: O94537 (Sensor for unfolded proteins in the ER ire1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=ire1 PE=1 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 4.5e-14
Identity = 44/123 (35.77%), Postives = 72/123 (58.54%), Query Frame = 0 

Query: 456 NSEKMDASISSEIENSLMQSENNWFQDNNLIGGRRIGKLIVTNKEIAKGSNGTVILEGIY 515
           +++ +  S+ S +++ L ++        N  G   +  L V  + I  GS+GT++  G+Y
Sbjct: 619 STDPLQKSLDSSLKSHLPEAT----VIQNTDGSVTVNSLTVYPEVIGYGSHGTIVYRGVY 678

Query: 516 EGRVVAVKRLVKAHHDVASKEVQNLMASDFHQNIVRWYGTESDQDFVYISLERCTCSLDD 575
           E R VAVKR++   +D+AS+E+  L  SD H NIVR+Y  +    F+YI +E C C+L D
Sbjct: 679 EDREVAVKRVLMEFYDLASREITLLQQSDNHPNIVRYYCKQKSDQFLYIVIELCKCNLSD 737

Query: 576 LIQ 579
           LI+
Sbjct: 739 LIE 737

[Close]
BLAST of Carg07912 vs. ExPASy TrEMBL
Match: A0A6J1EIH5 (Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111433677 PE=4 SV=1)

HSP 1 Score: 1231.5 bits (3185), Expect = 0.0e+00
Identity = 629/639 (98.44%), Postives = 633/639 (99.06%), Query Frame = 0 

Query: 1   MKCCSSFRYLWILLLILLRFWSYLGASRNFENSGFSGLSRYGGLDDSEAVARIGGRSLLS 60
           MKCCSSFRYLWILLLILLRFWSYLGASRNFENSGFSGLSRYGGLDDSEAVARIGGRSLLS
Sbjct: 1   MKCCSSFRYLWILLLILLRFWSYLGASRNFENSGFSGLSRYGGLDDSEAVARIGGRSLLS 60

Query: 61  VPTKGSTALIAALDGAIHLVDSNSMKIIWSFSSGPPIYSSHQAHVHHEQNQENASGLSRS 120
           VPTKGSTALIAALDGAIHLVDSNSMKIIWSFSSGPPIYSSHQAHVHHEQNQENASGLSRS
Sbjct: 61  VPTKGSTALIAALDGAIHLVDSNSMKIIWSFSSGPPIYSSHQAHVHHEQNQENASGLSRS 120

Query: 121 FFFYCGDDWELYMHTEYGETKLPKTIDEVVRSSPYIFEDGTVMTGSTKTTVFEVDHVTGE 180
           FFFYCGDDWELYMHTEYGETKLPKTIDEVVRSSPYIFEDGTVMTGSTKTTVFEVDHVTGE
Sbjct: 121 FFFYCGDDWELYMHTEYGETKLPKTIDEVVRSSPYIFEDGTVMTGSTKTTVFEVDHVTGE 180

Query: 181 LIRSHIPDLLSSELSNEDLGSSKAKNNLDDKDLMQPGVMNPPVEPRLYITRTDYSLKSSF 240
           LIRSHIPDLLSSELSNEDLGSSKAKNNLDDKDLMQPGVMNPPVEPRLYITRTDYSLKSSF
Sbjct: 181 LIRSHIPDLLSSELSNEDLGSSKAKNNLDDKDLMQPGVMNPPVEPRLYITRTDYSLKSSF 240

Query: 241 SGSKEVSWSLSVAEIGATLLCPEVENPVEGIPWNLQNNSSYEVDYAVPLSCQSKVLVFRE 300
           SGSKEVSWSLSVAEIGATLLCPEVENPVEGIPWNLQNNSSYEVDYAVPLSCQSKVLVFRE
Sbjct: 241 SGSKEVSWSLSVAEIGATLLCPEVENPVEGIPWNLQNNSSYEVDYAVPLSCQSKVLVFRE 300

Query: 301 RHVLSGKLGYKRLSKAHNVDNMSLVSASGSILPSQLKIDKHLNSHPERLMLPEPVSNNIS 360
           RHVLSGKLGYKRLSKAHNVDNMSLVSASGSILPSQLKIDKHLNSHPERLMLPEPVSNNIS
Sbjct: 301 RHVLSGKLGYKRLSKAHNVDNMSLVSASGSILPSQLKIDKHLNSHPERLMLPEPVSNNIS 360

Query: 361 SLPPNSSSEAVVPMPLMKVNESSIFKGFNKMETLAGPIGLLFVVLITMLVGLMKYGRTLA 420
           SLPPNSSSEAVVPMPLMKVNESSIFKGFNKMETLAGPIGLLFVVLITMLVGLMKYGRTLA
Sbjct: 361 SLPPNSSSEAVVPMPLMKVNESSIFKGFNKMETLAGPIGLLFVVLITMLVGLMKYGRTLA 420

Query: 421 VKVKQRFFNEKLSSTYNARIISSKKNKPRKSKRSGNSEKMDASISSEIENSLMQSENNWF 480
           VKVKQRFFNEKLSSTYNARIISSKKNKPRKSKRSGNSEKMDASISSEIENSLMQSENNWF
Sbjct: 421 VKVKQRFFNEKLSSTYNARIISSKKNKPRKSKRSGNSEKMDASISSEIENSLMQSENNWF 480

Query: 481 QDNNLIGGRRIGKLIVTNKEIAKGSNGTVILEGIYEGRVVAVKRLVKAHHDVASKEVQNL 540
           QDNNLIGGRRIGKLIVTNKEIAKGSNGTVILEGIYEGRVVAVKRLVKAHHDVASKEVQNL
Sbjct: 481 QDNNLIGGRRIGKLIVTNKEIAKGSNGTVILEGIYEGRVVAVKRLVKAHHDVASKEVQNL 540

Query: 541 MASDFHQNIVRWYGTESDQDFVYISLERCTCSLDDLIQICSDQSLNSMLGLDQDTVPMID 600
           MASDFHQNIVRWYGTESDQDFVYISLERCTCSLDDLIQICSDQSLNSMLGLDQDTVPMID
Sbjct: 541 MASDFHQNIVRWYGTESDQDFVYISLERCTCSLDDLIQICSDQSLNSMLGLDQDTVPMID 600

Query: 601 SVKEVIPDLKLWKENGHPSTVVLKLMRFSIFIIQHNNDL 640
           SVKEVIPDLKLWKENGHPSTVVLKLMR  +  + H ++L
Sbjct: 601 SVKEVIPDLKLWKENGHPSTVVLKLMRDMVAGLVHLHEL 639

[Close]
BLAST of Carg07912 vs. ExPASy TrEMBL
Match: A0A6J1KL94 (serine/threonine-protein kinase/endoribonuclease IRE1a-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111496759 PE=4 SV=1)

HSP 1 Score: 1188.7 bits (3074), Expect = 0.0e+00
Identity = 609/639 (95.31%), Postives = 618/639 (96.71%), Query Frame = 0 

Query: 1   MKCCSSFRYLWILLLILLRFWSYLGASRNFENSGFSGLSRYGGLDDSEAVARIGGRSLLS 60
           MK CSSFRYLWILLLILLRFWSYLGASRN ENSGFSGLSRYGG DDSEAVARIGGRSLLS
Sbjct: 1   MKRCSSFRYLWILLLILLRFWSYLGASRNSENSGFSGLSRYGGFDDSEAVARIGGRSLLS 60

Query: 61  VPTKGSTALIAALDGAIHLVDSNSMKIIWSFSSGPPIYSSHQAHVHHEQNQENASGLSRS 120
           VPTKGSTALIAALDGAIHLVDSNSMKIIWSFSSGPPIYS HQAHVHHE NQENASGLSR 
Sbjct: 61  VPTKGSTALIAALDGAIHLVDSNSMKIIWSFSSGPPIYSPHQAHVHHEHNQENASGLSRP 120

Query: 121 FFFYCGDDWELYMHTEYGETKLPKTIDEVVRSSPYIFEDGTVMTGSTKTTVFEVDHVTGE 180
           FFFYCGDDWELYMHTEYGETKLPKTIDEVVRS+PYIFEDGTVMTGSTKTTVFEVDHVTGE
Sbjct: 121 FFFYCGDDWELYMHTEYGETKLPKTIDEVVRSTPYIFEDGTVMTGSTKTTVFEVDHVTGE 180

Query: 181 LIRSHIPDLLSSELSNEDLGSSKAKNNLDDKDLMQPGVMNPPVEPRLYITRTDYSLKSSF 240
           LIRSHIPDLLSSELSNEDLGSSKAK+NLDDKDL+QPGVMNPPVEPRLYITRTDYSLKSSF
Sbjct: 181 LIRSHIPDLLSSELSNEDLGSSKAKSNLDDKDLIQPGVMNPPVEPRLYITRTDYSLKSSF 240

Query: 241 SGSKEVSWSLSVAEIGATLLCPEVENPVEGIPWNLQNNSSYEVDYAVPLSCQSKVLVFRE 300
           SGSKEVSWSLSVAEIGATLLCPEVENPVEGIPWNLQNNSSYEVDYAVPLSCQSKVLVFRE
Sbjct: 241 SGSKEVSWSLSVAEIGATLLCPEVENPVEGIPWNLQNNSSYEVDYAVPLSCQSKVLVFRE 300

Query: 301 RHVLSGKLGYKRLSKAHNVDNMSLVSASGSILPSQLKIDKHLNSHPERLMLPEPVSNNIS 360
           RH LSGK GYKRLSKAHNVDNMSLVSASGSILPSQLKIDKHLN HPERLMLPEPVSN IS
Sbjct: 301 RHALSGKSGYKRLSKAHNVDNMSLVSASGSILPSQLKIDKHLNIHPERLMLPEPVSNTIS 360

Query: 361 SLPPNSSSEAVVPMPLMKVNESSIFKGFNKMETLAGPIGLLFVVLITMLVGLMKYGRTLA 420
           SLPPNSSSEAVVPMPLMKVNESSIFKG NKMETLAGPIGL FVVLI MLVGLMKYGRTLA
Sbjct: 361 SLPPNSSSEAVVPMPLMKVNESSIFKGCNKMETLAGPIGLFFVVLIIMLVGLMKYGRTLA 420

Query: 421 VKVKQRFFNEKLSSTYNARIISSKKNKPRKSKRSGNSEKMDASISSEIENSLMQSENNWF 480
           VKVKQRFFNEK SST NARIISSKKNKPRKSKRSGNSEKMDASISSEIENSLMQSE+NWF
Sbjct: 421 VKVKQRFFNEKSSSTSNARIISSKKNKPRKSKRSGNSEKMDASISSEIENSLMQSEDNWF 480

Query: 481 QDNNLIGGRRIGKLIVTNKEIAKGSNGTVILEGIYEGRVVAVKRLVKAHHDVASKEVQNL 540
           QDNNLIGGRRIGKLIVTNKEIAKGSNGTVILEGIYEGRVVAVKRLVKAHHDVASKEVQNL
Sbjct: 481 QDNNLIGGRRIGKLIVTNKEIAKGSNGTVILEGIYEGRVVAVKRLVKAHHDVASKEVQNL 540

Query: 541 MASDFHQNIVRWYGTESDQDFVYISLERCTCSLDDLIQICSDQSLNSMLGLDQDTVPMID 600
           MASDFHQNIVRWYGTESDQDFVYISLERCTCSLDDLIQICSDQS NSMLGLDQDTVPMID
Sbjct: 541 MASDFHQNIVRWYGTESDQDFVYISLERCTCSLDDLIQICSDQSFNSMLGLDQDTVPMID 600

Query: 601 SVKEVIPDLKLWKENGHPSTVVLKLMRFSIFIIQHNNDL 640
           SVKEVIPDLKLWKENGHPSTVVLKLMR  +  ++H ++L
Sbjct: 601 SVKEVIPDLKLWKENGHPSTVVLKLMRDMVAGLEHLHEL 639

[Close]
BLAST of Carg07912 vs. ExPASy TrEMBL
Match: A0A6J1EFL5 (serine/threonine-protein kinase/endoribonuclease IRE1a-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111433677 PE=4 SV=1)

HSP 1 Score: 1071.2 bits (2769), Expect = 1.7e-309
Identity = 545/555 (98.20%), Postives = 549/555 (98.92%), Query Frame = 0 

Query: 85  MKIIWSFSSGPPIYSSHQAHVHHEQNQENASGLSRSFFFYCGDDWELYMHTEYGETKLPK 144
           MKIIWSFSSGPPIYSSHQAHVHHEQNQENASGLSRSFFFYCGDDWELYMHTEYGETKLPK
Sbjct: 1   MKIIWSFSSGPPIYSSHQAHVHHEQNQENASGLSRSFFFYCGDDWELYMHTEYGETKLPK 60

Query: 145 TIDEVVRSSPYIFEDGTVMTGSTKTTVFEVDHVTGELIRSHIPDLLSSELSNEDLGSSKA 204
           TIDEVVRSSPYIFEDGTVMTGSTKTTVFEVDHVTGELIRSHIPDLLSSELSNEDLGSSKA
Sbjct: 61  TIDEVVRSSPYIFEDGTVMTGSTKTTVFEVDHVTGELIRSHIPDLLSSELSNEDLGSSKA 120

Query: 205 KNNLDDKDLMQPGVMNPPVEPRLYITRTDYSLKSSFSGSKEVSWSLSVAEIGATLLCPEV 264
           KNNLDDKDLMQPGVMNPPVEPRLYITRTDYSLKSSFSGSKEVSWSLSVAEIGATLLCPEV
Sbjct: 121 KNNLDDKDLMQPGVMNPPVEPRLYITRTDYSLKSSFSGSKEVSWSLSVAEIGATLLCPEV 180

Query: 265 ENPVEGIPWNLQNNSSYEVDYAVPLSCQSKVLVFRERHVLSGKLGYKRLSKAHNVDNMSL 324
           ENPVEGIPWNLQNNSSYEVDYAVPLSCQSKVLVFRERHVLSGKLGYKRLSKAHNVDNMSL
Sbjct: 181 ENPVEGIPWNLQNNSSYEVDYAVPLSCQSKVLVFRERHVLSGKLGYKRLSKAHNVDNMSL 240

Query: 325 VSASGSILPSQLKIDKHLNSHPERLMLPEPVSNNISSLPPNSSSEAVVPMPLMKVNESSI 384
           VSASGSILPSQLKIDKHLNSHPERLMLPEPVSNNISSLPPNSSSEAVVPMPLMKVNESSI
Sbjct: 241 VSASGSILPSQLKIDKHLNSHPERLMLPEPVSNNISSLPPNSSSEAVVPMPLMKVNESSI 300

Query: 385 FKGFNKMETLAGPIGLLFVVLITMLVGLMKYGRTLAVKVKQRFFNEKLSSTYNARIISSK 444
           FKGFNKMETLAGPIGLLFVVLITMLVGLMKYGRTLAVKVKQRFFNEKLSSTYNARIISSK
Sbjct: 301 FKGFNKMETLAGPIGLLFVVLITMLVGLMKYGRTLAVKVKQRFFNEKLSSTYNARIISSK 360

Query: 445 KNKPRKSKRSGNSEKMDASISSEIENSLMQSENNWFQDNNLIGGRRIGKLIVTNKEIAKG 504
           KNKPRKSKRSGNSEKMDASISSEIENSLMQSENNWFQDNNLIGGRRIGKLIVTNKEIAKG
Sbjct: 361 KNKPRKSKRSGNSEKMDASISSEIENSLMQSENNWFQDNNLIGGRRIGKLIVTNKEIAKG 420

Query: 505 SNGTVILEGIYEGRVVAVKRLVKAHHDVASKEVQNLMASDFHQNIVRWYGTESDQDFVYI 564
           SNGTVILEGIYEGRVVAVKRLVKAHHDVASKEVQNLMASDFHQNIVRWYGTESDQDFVYI
Sbjct: 421 SNGTVILEGIYEGRVVAVKRLVKAHHDVASKEVQNLMASDFHQNIVRWYGTESDQDFVYI 480

Query: 565 SLERCTCSLDDLIQICSDQSLNSMLGLDQDTVPMIDSVKEVIPDLKLWKENGHPSTVVLK 624
           SLERCTCSLDDLIQICSDQSLNSMLGLDQDTVPMIDSVKEVIPDLKLWKENGHPSTVVLK
Sbjct: 481 SLERCTCSLDDLIQICSDQSLNSMLGLDQDTVPMIDSVKEVIPDLKLWKENGHPSTVVLK 540

Query: 625 LMRFSIFIIQHNNDL 640
           LMR  +  + H ++L
Sbjct: 541 LMRDMVAGLVHLHEL 555

[Close]
BLAST of Carg07912 vs. ExPASy TrEMBL
Match: A0A6J1KS56 (serine/threonine-protein kinase/endoribonuclease IRE1a-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111496759 PE=4 SV=1)

HSP 1 Score: 1037.7 bits (2682), Expect = 2.0e-299
Identity = 528/555 (95.14%), Postives = 537/555 (96.76%), Query Frame = 0 

Query: 85  MKIIWSFSSGPPIYSSHQAHVHHEQNQENASGLSRSFFFYCGDDWELYMHTEYGETKLPK 144
           MKIIWSFSSGPPIYS HQAHVHHE NQENASGLSR FFFYCGDDWELYMHTEYGETKLPK
Sbjct: 1   MKIIWSFSSGPPIYSPHQAHVHHEHNQENASGLSRPFFFYCGDDWELYMHTEYGETKLPK 60

Query: 145 TIDEVVRSSPYIFEDGTVMTGSTKTTVFEVDHVTGELIRSHIPDLLSSELSNEDLGSSKA 204
           TIDEVVRS+PYIFEDGTVMTGSTKTTVFEVDHVTGELIRSHIPDLLSSELSNEDLGSSKA
Sbjct: 61  TIDEVVRSTPYIFEDGTVMTGSTKTTVFEVDHVTGELIRSHIPDLLSSELSNEDLGSSKA 120

Query: 205 KNNLDDKDLMQPGVMNPPVEPRLYITRTDYSLKSSFSGSKEVSWSLSVAEIGATLLCPEV 264
           K+NLDDKDL+QPGVMNPPVEPRLYITRTDYSLKSSFSGSKEVSWSLSVAEIGATLLCPEV
Sbjct: 121 KSNLDDKDLIQPGVMNPPVEPRLYITRTDYSLKSSFSGSKEVSWSLSVAEIGATLLCPEV 180

Query: 265 ENPVEGIPWNLQNNSSYEVDYAVPLSCQSKVLVFRERHVLSGKLGYKRLSKAHNVDNMSL 324
           ENPVEGIPWNLQNNSSYEVDYAVPLSCQSKVLVFRERH LSGK GYKRLSKAHNVDNMSL
Sbjct: 181 ENPVEGIPWNLQNNSSYEVDYAVPLSCQSKVLVFRERHALSGKSGYKRLSKAHNVDNMSL 240

Query: 325 VSASGSILPSQLKIDKHLNSHPERLMLPEPVSNNISSLPPNSSSEAVVPMPLMKVNESSI 384
           VSASGSILPSQLKIDKHLN HPERLMLPEPVSN ISSLPPNSSSEAVVPMPLMKVNESSI
Sbjct: 241 VSASGSILPSQLKIDKHLNIHPERLMLPEPVSNTISSLPPNSSSEAVVPMPLMKVNESSI 300

Query: 385 FKGFNKMETLAGPIGLLFVVLITMLVGLMKYGRTLAVKVKQRFFNEKLSSTYNARIISSK 444
           FKG NKMETLAGPIGL FVVLI MLVGLMKYGRTLAVKVKQRFFNEK SST NARIISSK
Sbjct: 301 FKGCNKMETLAGPIGLFFVVLIIMLVGLMKYGRTLAVKVKQRFFNEKSSSTSNARIISSK 360

Query: 445 KNKPRKSKRSGNSEKMDASISSEIENSLMQSENNWFQDNNLIGGRRIGKLIVTNKEIAKG 504
           KNKPRKSKRSGNSEKMDASISSEIENSLMQSE+NWFQDNNLIGGRRIGKLIVTNKEIAKG
Sbjct: 361 KNKPRKSKRSGNSEKMDASISSEIENSLMQSEDNWFQDNNLIGGRRIGKLIVTNKEIAKG 420

Query: 505 SNGTVILEGIYEGRVVAVKRLVKAHHDVASKEVQNLMASDFHQNIVRWYGTESDQDFVYI 564
           SNGTVILEGIYEGRVVAVKRLVKAHHDVASKEVQNLMASDFHQNIVRWYGTESDQDFVYI
Sbjct: 421 SNGTVILEGIYEGRVVAVKRLVKAHHDVASKEVQNLMASDFHQNIVRWYGTESDQDFVYI 480

Query: 565 SLERCTCSLDDLIQICSDQSLNSMLGLDQDTVPMIDSVKEVIPDLKLWKENGHPSTVVLK 624
           SLERCTCSLDDLIQICSDQS NSMLGLDQDTVPMIDSVKEVIPDLKLWKENGHPSTVVLK
Sbjct: 481 SLERCTCSLDDLIQICSDQSFNSMLGLDQDTVPMIDSVKEVIPDLKLWKENGHPSTVVLK 540

Query: 625 LMRFSIFIIQHNNDL 640
           LMR  +  ++H ++L
Sbjct: 541 LMRDMVAGLEHLHEL 555

[Close]
BLAST of Carg07912 vs. ExPASy TrEMBL
Match: A0A6J1ELJ4 (serine/threonine-protein kinase/endoribonuclease IRE1a-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111433677 PE=4 SV=1)

HSP 1 Score: 949.5 bits (2453), Expect = 7.1e-273
Identity = 489/499 (98.00%), Postives = 493/499 (98.80%), Query Frame = 0 

Query: 141 KLPKTIDEVVRSSPYIFEDGTVMTGSTKTTVFEVDHVTGELIRSHIPDLLSSELSNEDLG 200
           KLPKTIDEVVRSSPYIFEDGTVMTGSTKTTVFEVDHVTGELIRSHIPDLLSSELSNEDLG
Sbjct: 13  KLPKTIDEVVRSSPYIFEDGTVMTGSTKTTVFEVDHVTGELIRSHIPDLLSSELSNEDLG 72

Query: 201 SSKAKNNLDDKDLMQPGVMNPPVEPRLYITRTDYSLKSSFSGSKEVSWSLSVAEIGATLL 260
           SSKAKNNLDDKDLMQPGVMNPPVEPRLYITRTDYSLKSSFSGSKEVSWSLSVAEIGATLL
Sbjct: 73  SSKAKNNLDDKDLMQPGVMNPPVEPRLYITRTDYSLKSSFSGSKEVSWSLSVAEIGATLL 132

Query: 261 CPEVENPVEGIPWNLQNNSSYEVDYAVPLSCQSKVLVFRERHVLSGKLGYKRLSKAHNVD 320
           CPEVENPVEGIPWNLQNNSSYEVDYAVPLSCQSKVLVFRERHVLSGKLGYKRLSKAHNVD
Sbjct: 133 CPEVENPVEGIPWNLQNNSSYEVDYAVPLSCQSKVLVFRERHVLSGKLGYKRLSKAHNVD 192

Query: 321 NMSLVSASGSILPSQLKIDKHLNSHPERLMLPEPVSNNISSLPPNSSSEAVVPMPLMKVN 380
           NMSLVSASGSILPSQLKIDKHLNSHPERLMLPEPVSNNISSLPPNSSSEAVVPMPLMKVN
Sbjct: 193 NMSLVSASGSILPSQLKIDKHLNSHPERLMLPEPVSNNISSLPPNSSSEAVVPMPLMKVN 252

Query: 381 ESSIFKGFNKMETLAGPIGLLFVVLITMLVGLMKYGRTLAVKVKQRFFNEKLSSTYNARI 440
           ESSIFKGFNKMETLAGPIGLLFVVLITMLVGLMKYGRTLAVKVKQRFFNEKLSSTYNARI
Sbjct: 253 ESSIFKGFNKMETLAGPIGLLFVVLITMLVGLMKYGRTLAVKVKQRFFNEKLSSTYNARI 312

Query: 441 ISSKKNKPRKSKRSGNSEKMDASISSEIENSLMQSENNWFQDNNLIGGRRIGKLIVTNKE 500
           ISSKKNKPRKSKRSGNSEKMDASISSEIENSLMQSENNWFQDNNLIGGRRIGKLIVTNKE
Sbjct: 313 ISSKKNKPRKSKRSGNSEKMDASISSEIENSLMQSENNWFQDNNLIGGRRIGKLIVTNKE 372

Query: 501 IAKGSNGTVILEGIYEGRVVAVKRLVKAHHDVASKEVQNLMASDFHQNIVRWYGTESDQD 560
           IAKGSNGTVILEGIYEGRVVAVKRLVKAHHDVASKEVQNLMASDFHQNIVRWYGTESDQD
Sbjct: 373 IAKGSNGTVILEGIYEGRVVAVKRLVKAHHDVASKEVQNLMASDFHQNIVRWYGTESDQD 432

Query: 561 FVYISLERCTCSLDDLIQICSDQSLNSMLGLDQDTVPMIDSVKEVIPDLKLWKENGHPST 620
           FVYISLERCTCSLDDLIQICSDQSLNSMLGLDQDTVPMIDSVKEVIPDLKLWKENGHPST
Sbjct: 433 FVYISLERCTCSLDDLIQICSDQSLNSMLGLDQDTVPMIDSVKEVIPDLKLWKENGHPST 492

Query: 621 VVLKLMRFSIFIIQHNNDL 640
           VVLKLMR  +  + H ++L
Sbjct: 493 VVLKLMRDMVAGLVHLHEL 511

[Close]
BLAST of Carg07912 vs. TAIR 10
Match: AT5G24360.1 (inositol requiring 1-1 )

HSP 1 Score: 252.3 bits (643), Expect = 1.0e-66
Identity = 205/594 (34.51%), Postives = 299/594 (50.34%), Query Frame = 0 

Query: 69  LIAALDGAIHLVDSNSMKIIWSFSSGPPIYSSHQA-HVHHEQNQENASGLSRSFFFYCGD 128
           L++ +DG+I LVD +S K+ W+F +  PIYSS+QA H H+  ++E +S L   F+  C  
Sbjct: 50  LVSTVDGSISLVDMSSQKLDWTFHTNEPIYSSYQAPHYHYTTDEERSSVLGDDFYMDCDK 109

Query: 129 DWELYMHTEYGETKLPKTID--EVVRSSPYIFEDGTVMTGSTKTTVFEVDHVTGELIRSH 188
           DW LY  +     ++ + +D  E + + PY   D  V+ G   T+VF +D  TG+L++ +
Sbjct: 110 DWRLYNSSVRKGKRVNEIVDASEFIGTLPYTSTDRIVL-GKKDTSVFLLDWKTGKLVKRY 169

Query: 189 IPDLLSSELSNEDLGSSKAKNNLDDKD---LMQPGVMNPPVEPRL-YITRTDYSLKSSFS 248
             D L    SN  + + K K  +  K+   L   G       P L YI R D+ ++   S
Sbjct: 170 RMDEL---YSNTVVENDKEKAIVLSKEAPLLFGSGFKKSEDFPELVYIERKDFKIQ-CIS 229

Query: 249 GSKEVSWSLSVAEIGATLLCPEVENPVEGIPWNLQNNSSYEVDYAVPLSCQSKVLVFRER 308
              +V WS+S A++ A L   E    + G+  ++  N         PLS  + V + + R
Sbjct: 230 KFGDVLWSVSYAKMEAKLQNHESVQFISGLSSSVGKNQ-------FPLSYTTSVPMVQLR 289

Query: 309 HVLSGKLGYKRLSKAHNVDNMSLVSASGSILPSQLKIDKHLNSHPERLMLPEPVSNNISS 368
           +V      Y+ L       + +L        P+QL I                   N  +
Sbjct: 290 NV-----KYETLFPRLGFLDEALYLPFQDRKPNQLAIG----------------DGNQLT 349

Query: 369 LPPNSSSEAVVPMPLMKV---NESSIFKGFNKMETLA----GPIGLLFVVLITML--VGL 428
           LP N  +E V+ +PL +      + I  G  K    A    G I L+F   +TML   GL
Sbjct: 350 LPGNKEAEEVLSLPLPETVISQITDIIDGSTKQAGFASKFSGLIVLIFGFCVTMLSVCGL 409

Query: 429 MKYGRTLAVKVKQRFFNEKLSSTYNARIISSKKNKPRKSKRSGNSEKMDASISSEIENSL 488
             Y    ++++K+ + +E         I + KK K +K+  +    K +    S      
Sbjct: 410 FFYRLRQSIRIKEPYVSE-------VPIATPKKKKSKKNGTTKAVHKKENGFISGGNKDP 469

Query: 489 MQSENNW-------FQDNNLIGGRRIGKLIVTNKEIAKGSNGTVILEGIYEGRVVAVKRL 548
              EN           +N+   G R+GKL V+NKEIAKGSNGTV+LEG YEGR+VAVKRL
Sbjct: 470 SHEENEKRLLTAFPGLNNSSAEGYRVGKLFVSNKEIAKGSNGTVVLEGSYEGRLVAVKRL 529

Query: 549 VKAHHDVASKEVQNLMASDFHQNIVRWYGTESDQDFVYISLERCTCSLDDLIQICSDQSL 608
           V++HHDVA KE+ NLMASD H NIVRWYG + D+ F+YISLE C CSL+DLI   S   L
Sbjct: 530 VQSHHDVAQKEILNLMASDKHSNIVRWYGVDQDEHFIYISLELCACSLNDLI-YASSALL 589

Query: 609 NSMLGLDQDTVPMIDSVKEVIPDLKLWKENGHPSTVVLKLMRFSIFIIQHNNDL 640
            S +         I+ + E    ++LWKENGHPS V+LKLMR  +  + H +D+
Sbjct: 590 ESPMASSSIHSIQINPIFENGKGVELWKENGHPSPVLLKLMRDIVAGLVHLHDI 602

[Close]
BLAST of Carg07912 vs. TAIR 10
Match: AT5G24360.2 (inositol requiring 1-1 )

HSP 1 Score: 252.3 bits (643), Expect = 1.0e-66
Identity = 205/594 (34.51%), Postives = 299/594 (50.34%), Query Frame = 0 

Query: 69  LIAALDGAIHLVDSNSMKIIWSFSSGPPIYSSHQA-HVHHEQNQENASGLSRSFFFYCGD 128
           L++ +DG+I LVD +S K+ W+F +  PIYSS+QA H H+  ++E +S L   F+  C  
Sbjct: 56  LVSTVDGSISLVDMSSQKLDWTFHTNEPIYSSYQAPHYHYTTDEERSSVLGDDFYMDCDK 115

Query: 129 DWELYMHTEYGETKLPKTID--EVVRSSPYIFEDGTVMTGSTKTTVFEVDHVTGELIRSH 188
           DW LY  +     ++ + +D  E + + PY   D  V+ G   T+VF +D  TG+L++ +
Sbjct: 116 DWRLYNSSVRKGKRVNEIVDASEFIGTLPYTSTDRIVL-GKKDTSVFLLDWKTGKLVKRY 175

Query: 189 IPDLLSSELSNEDLGSSKAKNNLDDKD---LMQPGVMNPPVEPRL-YITRTDYSLKSSFS 248
             D L    SN  + + K K  +  K+   L   G       P L YI R D+ ++   S
Sbjct: 176 RMDEL---YSNTVVENDKEKAIVLSKEAPLLFGSGFKKSEDFPELVYIERKDFKIQ-CIS 235

Query: 249 GSKEVSWSLSVAEIGATLLCPEVENPVEGIPWNLQNNSSYEVDYAVPLSCQSKVLVFRER 308
              +V WS+S A++ A L   E    + G+  ++  N         PLS  + V + + R
Sbjct: 236 KFGDVLWSVSYAKMEAKLQNHESVQFISGLSSSVGKNQ-------FPLSYTTSVPMVQLR 295

Query: 309 HVLSGKLGYKRLSKAHNVDNMSLVSASGSILPSQLKIDKHLNSHPERLMLPEPVSNNISS 368
           +V      Y+ L       + +L        P+QL I                   N  +
Sbjct: 296 NV-----KYETLFPRLGFLDEALYLPFQDRKPNQLAIG----------------DGNQLT 355

Query: 369 LPPNSSSEAVVPMPLMKV---NESSIFKGFNKMETLA----GPIGLLFVVLITML--VGL 428
           LP N  +E V+ +PL +      + I  G  K    A    G I L+F   +TML   GL
Sbjct: 356 LPGNKEAEEVLSLPLPETVISQITDIIDGSTKQAGFASKFSGLIVLIFGFCVTMLSVCGL 415

Query: 429 MKYGRTLAVKVKQRFFNEKLSSTYNARIISSKKNKPRKSKRSGNSEKMDASISSEIENSL 488
             Y    ++++K+ + +E         I + KK K +K+  +    K +    S      
Sbjct: 416 FFYRLRQSIRIKEPYVSE-------VPIATPKKKKSKKNGTTKAVHKKENGFISGGNKDP 475

Query: 489 MQSENNW-------FQDNNLIGGRRIGKLIVTNKEIAKGSNGTVILEGIYEGRVVAVKRL 548
              EN           +N+   G R+GKL V+NKEIAKGSNGTV+LEG YEGR+VAVKRL
Sbjct: 476 SHEENEKRLLTAFPGLNNSSAEGYRVGKLFVSNKEIAKGSNGTVVLEGSYEGRLVAVKRL 535

Query: 549 VKAHHDVASKEVQNLMASDFHQNIVRWYGTESDQDFVYISLERCTCSLDDLIQICSDQSL 608
           V++HHDVA KE+ NLMASD H NIVRWYG + D+ F+YISLE C CSL+DLI   S   L
Sbjct: 536 VQSHHDVAQKEILNLMASDKHSNIVRWYGVDQDEHFIYISLELCACSLNDLI-YASSALL 595

Query: 609 NSMLGLDQDTVPMIDSVKEVIPDLKLWKENGHPSTVVLKLMRFSIFIIQHNNDL 640
            S +         I+ + E    ++LWKENGHPS V+LKLMR  +  + H +D+
Sbjct: 596 ESPMASSSIHSIQINPIFENGKGVELWKENGHPSPVLLKLMRDIVAGLVHLHDI 608

[Close]
BLAST of Carg07912 vs. TAIR 10
Match: AT5G24360.3 (inositol requiring 1-1 )

HSP 1 Score: 252.3 bits (643), Expect = 1.0e-66
Identity = 206/602 (34.22%), Postives = 301/602 (50.00%), Query Frame = 0 

Query: 61  VPTKGSTALIAALDGAIHLVDSNSMKIIWSFSSGPPIYSSHQA-HVHHEQNQENASGLSR 120
           V  +    L++ +DG+I LVD +S K+ W+F +  PIYSS+QA H H+  ++E +S L  
Sbjct: 28  VSKESGYVLVSTVDGSISLVDMSSQKLDWTFHTNEPIYSSYQAPHYHYTTDEERSSVLGD 87

Query: 121 SFFFYCGDDWELYMHTEYGETKLPKTID--EVVRSSPYIFEDGTVMTGSTKTTVFEVDHV 180
            F+  C  DW LY  +     ++ + +D  E + + PY   D  V+ G   T+VF +D  
Sbjct: 88  DFYMDCDKDWRLYNSSVRKGKRVNEIVDASEFIGTLPYTSTDRIVL-GKKDTSVFLLDWK 147

Query: 181 TGELIRSHIPDLLSSELSNEDLGSSKAKNNLDDKD---LMQPGVMNPPVEPRL-YITRTD 240
           TG+L++ +  D L    SN  + + K K  +  K+   L   G       P L YI R D
Sbjct: 148 TGKLVKRYRMDEL---YSNTVVENDKEKAIVLSKEAPLLFGSGFKKSEDFPELVYIERKD 207

Query: 241 YSLKSSFSGSKEVSWSLSVAEIGATLLCPEVENPVEGIPWNLQNNSSYEVDYAVPLSCQS 300
           + ++   S   +V WS+S A++ A L   E    + G+  ++  N         PLS  +
Sbjct: 208 FKIQ-CISKFGDVLWSVSYAKMEAKLQNHESVQFISGLSSSVGKNQ-------FPLSYTT 267

Query: 301 KVLVFRERHVLSGKLGYKRLSKAHNVDNMSLVSASGSILPSQLKIDKHLNSHPERLMLPE 360
            V + + R+V      Y+ L       + +L        P+QL I               
Sbjct: 268 SVPMVQLRNV-----KYETLFPRLGFLDEALYLPFQDRKPNQLAIG-------------- 327

Query: 361 PVSNNISSLPPNSSSEAVVPMPLMKV---NESSIFKGFNKMETLA----GPIGLLFVVLI 420
               N  +LP N  +E V+ +PL +      + I  G  K    A    G I L+F   +
Sbjct: 328 --DGNQLTLPGNKEAEEVLSLPLPETVISQITDIIDGSTKQAGFASKFSGLIVLIFGFCV 387

Query: 421 TML--VGLMKYGRTLAVKVKQRFFNEKLSSTYNARIISSKKNKPRKSKRSGNSEKMDASI 480
           TML   GL  Y    ++++K+ + +E         I + KK K +K+  +    K +   
Sbjct: 388 TMLSVCGLFFYRLRQSIRIKEPYVSE-------VPIATPKKKKSKKNGTTKAVHKKENGF 447

Query: 481 SSEIENSLMQSENNW-------FQDNNLIGGRRIGKLIVTNKEIAKGSNGTVILEGIYEG 540
            S         EN           +N+   G R+GKL V+NKEIAKGSNGTV+LEG YEG
Sbjct: 448 ISGGNKDPSHEENEKRLLTAFPGLNNSSAEGYRVGKLFVSNKEIAKGSNGTVVLEGSYEG 507

Query: 541 RVVAVKRLVKAHHDVASKEVQNLMASDFHQNIVRWYGTESDQDFVYISLERCTCSLDDLI 600
           R+VAVKRLV++HHDVA KE+ NLMASD H NIVRWYG + D+ F+YISLE C CSL+DLI
Sbjct: 508 RLVAVKRLVQSHHDVAQKEILNLMASDKHSNIVRWYGVDQDEHFIYISLELCACSLNDLI 567

Query: 601 QICSDQSLNSMLGLDQDTVPMIDSVKEVIPDLKLWKENGHPSTVVLKLMRFSIFIIQHNN 640
              S   L S +         I+ + E    ++LWKENGHPS V+LKLMR  +  + H +
Sbjct: 568 -YASSALLESPMASSSIHSIQINPIFENGKGVELWKENGHPSPVLLKLMRDIVAGLVHLH 588

[Close]
BLAST of Carg07912 vs. TAIR 10
Match: AT2G17520.1 (Endoribonuclease/protein kinase IRE1-like )

HSP 1 Score: 223.8 bits (569), Expect = 4.0e-58
Identity = 209/660 (31.67%), Postives = 304/660 (46.06%), Query Frame = 0 

Query: 4   CSSFRYLWILLLILLRFWSYLGASRNFENSGFSGLSRYGGLDDSEAVARI----GGRSLL 63
           C   R+L+ LLL LL  W                +S  GG  D      +    G RS+L
Sbjct: 5   CPFLRHLFFLLL-LLSPWI---------------MSPCGGAADDVTYPIVPSSPGRRSIL 64

Query: 64  SV----PTKGSTALIAALDGAIHLVDSNSMKIIWSFSSGPPIYSSHQAHVHHEQNQENAS 123
            +    PT+ +T L+   DG + L         WSFS+G P++S +QA  ++  N ENA+
Sbjct: 65  QIRREPPTEPNTKLVVDRDGKVFLKQQPKETPYWSFSTGSPMHSLYQAPANN--NTENAT 124

Query: 124 GLSRSFFFYCGDDWELYMHTEYGETKLPK----TIDEVVRSSPYIFEDGTVMTGSTKTTV 183
            ++R            Y++     T +      T+ E  R  P + +DG V  GS  T+ 
Sbjct: 125 EITRPHIIV------EYLNNSKAATTVDGYHNWTVQEFFRQKPLVTDDG-VTLGSETTSA 184

Query: 184 FEVDHVTGELIRSHIPDLLSSELSNEDLGSSKAKNNLDDKDLMQPGVMNPPVEPRLYITR 243
           + VD  +G LI  +           +  G +K  N      L++P      V   L I R
Sbjct: 185 YLVDGRSGRLIHVY-----------KSTGDTKITN-----ALVKPASTEDFVNEPLLIRR 244

Query: 244 TDYSLKSSFSGSKEVSWSLSVAEIGATLLCPEVENPVEGIPWNLQNNSSYEVDYAVPLSC 303
           TD  L+     + ++ W+L+V+   A LLC  V N    +   LQ          +PL C
Sbjct: 245 TDSKLEHFSKTTGKLVWNLTVSHFRAALLCDPVFNSGYDLGPKLQTG------IYMPLLC 304

Query: 304 QSKVLVFRERHVLSGKLGYKRLSKAHNVDNMSLVSASGSILPSQLKIDKHLNSHPERLML 363
            S+                              +   G  +  ++  D+ +N      ML
Sbjct: 305 GSQ------------------------------IDVRGPEIVIRVLHDQPMNVK----ML 364

Query: 364 PEPVSNNISS----LPPNSSSEAVVPMPLMKVNESSIFKGFNKMETLAGPIGLLFVVLIT 423
           P P  N+  S    +P   + E+       K   + +F  ++ ++ LA P+ LL VV+  
Sbjct: 365 PSPSLNHFESENSIMPFGKARESRKLQEQHKQKYTYLFGQWSPVKLLA-PLVLLGVVVSV 424

Query: 424 MLVGLMKYGRTLAVKVKQRFFNEKLSSTYNARIISSKKNKPRKSKRSGNSEKMDASIS-- 483
            +      G  +++K                    SKK K RKS +  N + +       
Sbjct: 425 FIKKFSSRGSDVSLKAG-----------------PSKKKKNRKSAKDTNRQSVPRGQDQF 484

Query: 484 SEIENSLMQSENNWFQDNNLIGGRRIGKLIVTNKEIAKGSNGTVILEGIYEGRVVAVKRL 543
             IE   M    N FQ +    GR+IGKL +++KEIAKGSNGTV+ EGIYEGR VAVKRL
Sbjct: 485 ELIEGGQMLLGFNNFQ-SGATDGRKIGKLFLSSKEIAKGSNGTVVFEGIYEGRPVAVKRL 544

Query: 544 VKAHHDVASKEVQNLMASDFHQNIVRWYGTESDQDFVYISLERCTCSLDDLIQICSDQSL 603
           V++HH+VA KE+QNL+ASD H NI+RWYG E DQDFVY+SLERCTCSLDDLI+   + S+
Sbjct: 545 VRSHHEVAFKEIQNLIASDQHTNIIRWYGVEYDQDFVYLSLERCTCSLDDLIKSYLEFSM 564

Query: 604 NSMLGLDQDTVPM------IDSVKEVIPDLKLWKENGHPSTVVLKLMRFSIFIIQHNNDL 640
             +L  +  T  +      +DS++ VI     WK  GHPS ++LKLMR  +  I H ++L
Sbjct: 605 TKVLENNDSTEGVAAYKIQLDSLEGVIKGNNFWKVGGHPSPLMLKLMRDIVCGIVHLHEL 564

[Close]
BLAST of Carg07912 vs. TAIR 10
Match: AT3G11870.1 (Endoribonuclease/protein kinase IRE1-like )

HSP 1 Score: 84.0 bits (206), Expect = 4.9e-16
Identity = 82/262 (31.30%), Postives = 120/262 (45.80%), Query Frame = 0 

Query: 398 IGLLFVVLITMLVGLMKYGRTLAVKVKQRFFNEKLSSTYNARIISSKKNKPRKSKRSG-- 457
           +GLL VV+   L      G    V  K+R  N     + +    +  +   +KS  SG  
Sbjct: 9   VGLLVVVVCVFLRFSKDKGLDGIVNEKKRDKNSAPRVSASGEDGTKNEQVEKKSDPSGGL 68

Query: 458 -------NSEK------MDASISSEIENSLMQSENNWFQDNNLIGGRRIGKLIVTNKEIA 517
                  NSE        D +I+  +   L   E   + +N   G     +L+V+  E+ 
Sbjct: 69  GEENEKTNSESKVLSVPSDQNINKTLPVMLPSLELRKYDENETPGKVVNRRLLVSTNEMK 128

Query: 518 KGSNGTVILEGIYEGR-VVAVKRLVKAHHDVA--SKEVQNLMASDFHQNIVRWYGTESDQ 577
            G NG  + +G+Y  R  VAVK L  AH   A    E+ N    D H NI+R++G E DQ
Sbjct: 129 YGRNGYEVFQGVYGRRSSVAVKCLDLAHTTEAFIQNEIDNHCLCDDHSNIIRFHGLEQDQ 188

Query: 578 DFVYISLERCTCSLDDLIQICSDQSLNSMLGLDQDTVPMIDSVKEVIPDLKLWKENGH-- 637
            F YI LE   CSLDDLI++   ++       D   V  +D +++V+  +K WKE G   
Sbjct: 189 SFAYICLEPWKCSLDDLIKLSVRRTKR-----DTQAVAPVDDLEKVMKRIKFWKEKGKPL 248

Query: 638 PSTVVLKLMRFSIFIIQHNNDL 640
           P T +LKLMR  +  + H + L
Sbjct: 249 PLTPMLKLMRDVVCGLAHLHKL 265

The following BLAST results are available for this feature:
BLAST of Carg07912 vs. NCBI nr
Match NameE-valueIdentityDescription
KAG7026699.10.0e+00100.00Serine/threonine-protein kinase/endoribonuclease IRE1b [Cucurbita argyrosperma s... [more]
XP_022926588.10.0e+0098.44serine/threonine-protein kinase/endoribonuclease IRE1a-like isoform X1 [Cucurbit... [more]
KAG6594729.10.0e+0096.87Serine/threonine-protein kinase/endoribonuclease IRE1b, partial [Cucurbita argyr... [more]
XP_023518030.10.0e+0095.77serine/threonine-protein kinase/endoribonuclease IRE1a-like isoform X1 [Cucurbit... [more]
XP_023003027.10.0e+0095.31serine/threonine-protein kinase/endoribonuclease IRE1a-like isoform X1 [Cucurbit... [more]
BLAST of Carg07912 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q93VJ21.5e-6534.51Serine/threonine-protein kinase/endoribonuclease IRE1b OS=Arabidopsis thaliana O... [more]
Q9C5S25.6e-5731.67Serine/threonine-protein kinase/endoribonuclease IRE1a OS=Arabidopsis thaliana O... [more]
Q7XIT14.3e-4129.62Serine/threonine-protein kinase/endoribonuclease IRE1 OS=Oryza sativa subsp. jap... [more]
Q9SF126.9e-1531.30Inactive serine/threonine-protein kinase/endoribonuclease IRE1-like OS=Arabidops... [more]
O945374.5e-1435.77Sensor for unfolded proteins in the ER ire1 OS=Schizosaccharomyces pombe (strain... [more]
BLAST of Carg07912 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1EIH50.0e+0098.44Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1KL940.0e+0095.31serine/threonine-protein kinase/endoribonuclease IRE1a-like isoform X1 OS=Cucurb... [more]
A0A6J1EFL51.7e-30998.20serine/threonine-protein kinase/endoribonuclease IRE1a-like isoform X2 OS=Cucurb... [more]
A0A6J1KS562.0e-29995.14serine/threonine-protein kinase/endoribonuclease IRE1a-like isoform X2 OS=Cucurb... [more]
A0A6J1ELJ47.1e-27398.00serine/threonine-protein kinase/endoribonuclease IRE1a-like isoform X3 OS=Cucurb... [more]
BLAST of Carg07912 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G24360.11.0e-6634.51inositol requiring 1-1 [more]
AT5G24360.21.0e-6634.51inositol requiring 1-1 [more]
AT5G24360.31.0e-6634.22inositol requiring 1-1 [more]
AT2G17520.14.0e-5831.67Endoribonuclease/protein kinase IRE1-like [more]
AT3G11870.14.9e-1631.30Endoribonuclease/protein kinase IRE1-like [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 463..610
e-value: 3.9E-28
score: 100.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 441..463
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 196..216
NoneNo IPR availablePANTHERPTHR13954:SF26SERINE/THREONINE KINASE DOMAIN PROTEINcoord: 49..628
IPR045133Serine/threonine-protein kinase/endoribonuclease IRE1/2-likePANTHERPTHR13954IRE1-RELATEDcoord: 49..628
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 495..639
score: 10.025966
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 487..585
IPR011047Quinoprotein alcohol dehydrogenase-like superfamilySUPERFAMILY50998Quinoprotein alcohol dehydrogenase-likecoord: 65..186

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg07912-RACarg07912-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0036498 IRE1-mediated unfolded protein response
biological_process GO:0006397 mRNA processing
biological_process GO:0006468 protein phosphorylation
biological_process GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic
biological_process GO:0030968 endoplasmic reticulum unfolded protein response
cellular_component GO:0016021 integral component of membrane
cellular_component GO:1990604 IRE1-TRAF2-ASK1 complex
molecular_function GO:0005524 ATP binding
molecular_function GO:0004521 endoribonuclease activity
molecular_function GO:0106310 protein serine kinase activity
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004712 protein serine/threonine/tyrosine kinase activity
molecular_function GO:0051082 unfolded protein binding
molecular_function GO:0004672 protein kinase activity