Carg07887 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg07887
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	SNF1-related protein kinase regulatory subunit beta-2-like
Location	Carg_Chr07: 1580677 .. 1581568 (-)
RNA-Seq Expression	Carg07887
Synteny	Carg07887

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCTAATGTCTTGTTCTTGATCAGAATACCCTTGCAGAGATCTGGGAAGGACTTCACGATTGTGAAGGTACTGCCTTCTGGTGTTTACCAGTATAGGTTTATTGCTGATGGACAATGGAGATATGCTCCTGACTTACCTTGGGCTCGAGATGATTCCGGCAATGCTTACAATATCTTGGACTTGCAGGTAAAAACATCTCTTTTTTGCACACCACTGCTTATGAATGGAAGTAGGACAACCTATTACCATAGCTGATTTTACTGTTGCAGGACTATGTCCCAGAAGACATTGAAAGCATTTCTAGCTTCGAACCTCCTCAATCCCCGGAGTCGAGTTACAGCAGTTTGCAGCTTGGATCTGAAGATTTTTCGAAGGAGCCACCATTGGCTCCTCCTCACTTGAAATCCACGTTGCTTGACATGCCTTGCCCATACAATGAGATTTTGCCTCCTTTGTCAAGACCTCAGCATGTAATGCTGAATCATCTTTATATGCAGAAGGAAAGGGGCGGTCCATCTGTCGTGGCACTCGGGATGACTCACCGGTTTCTAGCAAAGTACGTAACTGTTGTCCTCTATAAATCGTTGCAGAGATAATGTAGTATAGGCATATCCTGTGTGAAAGAAATTCAAAATCTAAACCCATTTTTCCTTACTGTAGCTTTATATGGTGTTGATGCCATTTCATTTTAACACTTCTGAATTAACCAGGACGTCTGAATGGATCGTTCTTCAAGACTCTACCTTACATGATGGCATCTCTTTTTGATCTGACATGCATTTTAACCATTTAGCAACCTTTTCCCCATCATTGTCGTAGGATCTTTCGTTTCACTCCCTTTCTACCTATCATGTAGCTTTTGCTAGTGAACCAATCTATTCGGGATGCC

mRNA sequence

ATGCCTAATGTCTTGTTCTTGATCAGAATACCCTTGCAGAGATCTGGGAAGGACTTCACGATTGTGAAGGTACTGCCTTCTGGTGTTTACCAGTATAGGTTTATTGCTGATGGACAATGGAGATATGCTCCTGACTTACCTTGGGCTCGAGATGATTCCGGCAATGCTTACAATATCTTGGACTTGCAGGACTATGTCCCAGAAGACATTGAAAGCATTTCTAGCTTCGAACCTCCTCAATCCCCGGAGTCGAGTTACAGCAGTTTGCAGCTTGGATCTGAAGATTTTTCGAAGGAGCCACCATTGGCTCCTCCTCACTTGAAATCCACGTTGCTTGACATGCCTTGCCCATACAATGAGATTTTGCCTCCTTTGTCAAGACCTCAGCATGTAATGCTGAATCATCTTTATATGCAGAAGGAAAGGGGCGGTCCATCTGTCGTGGCACTCGGGATGACTCACCGGTTTCTAGCAAAGTACGTAACTGTTGTCCTCTATAAATCGTTGCAGAGATAATGTAGTATAGGCATATCCTGTGTGAAAGAAATTCAAAATCTAAACCCATTTTTCCTTACTGTAGCTTTATATGGTGTTGATGCCATTTCATTTTAACACTTCTGAATTAACCAGGACGTCTGAATGGATCGTTCTTCAAGACTCTACCTTACATGATGGCATCTCTTTTTGATCTGACATGCATTTTAACCATTTAGCAACCTTTTCCCCATCATTGTCGTAGGATCTTTCGTTTCACTCCCTTTCTACCTATCATGTAGCTTTTGCTAGTGAACCAATCTATTCGGGATGCC

Coding sequence (CDS)

ATGCCTAATGTCTTGTTCTTGATCAGAATACCCTTGCAGAGATCTGGGAAGGACTTCACGATTGTGAAGGTACTGCCTTCTGGTGTTTACCAGTATAGGTTTATTGCTGATGGACAATGGAGATATGCTCCTGACTTACCTTGGGCTCGAGATGATTCCGGCAATGCTTACAATATCTTGGACTTGCAGGACTATGTCCCAGAAGACATTGAAAGCATTTCTAGCTTCGAACCTCCTCAATCCCCGGAGTCGAGTTACAGCAGTTTGCAGCTTGGATCTGAAGATTTTTCGAAGGAGCCACCATTGGCTCCTCCTCACTTGAAATCCACGTTGCTTGACATGCCTTGCCCATACAATGAGATTTTGCCTCCTTTGTCAAGACCTCAGCATGTAATGCTGAATCATCTTTATATGCAGAAGGAAAGGGGCGGTCCATCTGTCGTGGCACTCGGGATGACTCACCGGTTTCTAGCAAAGTACGTAACTGTTGTCCTCTATAAATCGTTGCAGAGATAA

Protein sequence

MPNVLFLIRIPLQRSGKDFTIVKVLPSGVYQYRFIADGQWRYAPDLPWARDDSGNAYNILDLQDYVPEDIESISSFEPPQSPESSYSSLQLGSEDFSKEPPLAPPHLKSTLLDMPCPYNEILPPLSRPQHVMLNHLYMQKERGGPSVVALGMTHRFLAKYVTVVLYKSLQR

Homology

[Close]

BLAST of Carg07887 vs. NCBI nr
Match: KAG7026674.1 (SNF1-related protein kinase regulatory subunit beta-2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 350.5 bits (898), Expect = 8.1e-93
Identity = 171/171 (100.00%), Postives = 171/171 (100.00%), Query Frame = 0


Query: 1   MPNVLFLIRIPLQRSGKDFTIVKVLPSGVYQYRFIADGQWRYAPDLPWARDDSGNAYNIL 60
           MPNVLFLIRIPLQRSGKDFTIVKVLPSGVYQYRFIADGQWRYAPDLPWARDDSGNAYNIL
Sbjct: 1   MPNVLFLIRIPLQRSGKDFTIVKVLPSGVYQYRFIADGQWRYAPDLPWARDDSGNAYNIL 60

Query: 61  DLQDYVPEDIESISSFEPPQSPESSYSSLQLGSEDFSKEPPLAPPHLKSTLLDMPCPYNE 120
           DLQDYVPEDIESISSFEPPQSPESSYSSLQLGSEDFSKEPPLAPPHLKSTLLDMPCPYNE
Sbjct: 61  DLQDYVPEDIESISSFEPPQSPESSYSSLQLGSEDFSKEPPLAPPHLKSTLLDMPCPYNE 120

Query: 121 ILPPLSRPQHVMLNHLYMQKERGGPSVVALGMTHRFLAKYVTVVLYKSLQR 172
           ILPPLSRPQHVMLNHLYMQKERGGPSVVALGMTHRFLAKYVTVVLYKSLQR
Sbjct: 121 ILPPLSRPQHVMLNHLYMQKERGGPSVVALGMTHRFLAKYVTVVLYKSLQR 171

[Close]

BLAST of Carg07887 vs. NCBI nr
Match: XP_022926790.1 (SNF1-related protein kinase regulatory subunit beta-2-like [Cucurbita moschata])

HSP 1 Score: 333.6 bits (854), Expect = 1.0e-87
Identity = 161/164 (98.17%), Postives = 164/164 (100.00%), Query Frame = 0


Query: 8   IRIPLQRSGKDFTIVKVLPSGVYQYRFIADGQWRYAPDLPWARDDSGNAYNILDLQDYVP 67
           ++IPLQRSGKDFTIVKVLPSGVYQYRFIADGQWRYAPDLPWA+DDSGNAYNILDLQDYVP
Sbjct: 138 MKIPLQRSGKDFTIVKVLPSGVYQYRFIADGQWRYAPDLPWAQDDSGNAYNILDLQDYVP 197

Query: 68  EDIESISSFEPPQSPESSYSSLQLGSEDFSKEPPLAPPHLKSTLLDMPCPYNEILPPLSR 127
           EDIESISSFEPPQSPESSYSSLQLGSEDFSKEPPLAPPHLKSTLLDMPCPYNEILPPLSR
Sbjct: 198 EDIESISSFEPPQSPESSYSSLQLGSEDFSKEPPLAPPHLKSTLLDMPCPYNEILPPLSR 257

Query: 128 PQHVMLNHLYMQKERGGPSVVALGMTHRFLAKYVTVVLYKSLQR 172
           PQHVMLNHLYMQKERGGPSVVALGMTHRFLAKYVTVVLYKSLQR
Sbjct: 258 PQHVMLNHLYMQKERGGPSVVALGMTHRFLAKYVTVVLYKSLQR 301

[Close]

BLAST of Carg07887 vs. NCBI nr
Match: XP_023517360.1 (SNF1-related protein kinase regulatory subunit beta-2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 333.6 bits (854), Expect = 1.0e-87
Identity = 161/164 (98.17%), Postives = 164/164 (100.00%), Query Frame = 0


Query: 8   IRIPLQRSGKDFTIVKVLPSGVYQYRFIADGQWRYAPDLPWARDDSGNAYNILDLQDYVP 67
           ++IPLQRSGKDFTIVKVLPSGVYQYRFIADGQWRYAPDLPWA+DDSGNAYNILDLQDYVP
Sbjct: 138 MKIPLQRSGKDFTIVKVLPSGVYQYRFIADGQWRYAPDLPWAQDDSGNAYNILDLQDYVP 197

Query: 68  EDIESISSFEPPQSPESSYSSLQLGSEDFSKEPPLAPPHLKSTLLDMPCPYNEILPPLSR 127
           EDIESISSFEPPQSPESSYSSLQLGSEDFSKEPPLAPPHLKSTLLDMPCPYNEILPPLSR
Sbjct: 198 EDIESISSFEPPQSPESSYSSLQLGSEDFSKEPPLAPPHLKSTLLDMPCPYNEILPPLSR 257

Query: 128 PQHVMLNHLYMQKERGGPSVVALGMTHRFLAKYVTVVLYKSLQR 172
           PQHVMLNHLYMQKERGGPSVVALGMTHRFLAKYVTVVLYKSLQR
Sbjct: 258 PQHVMLNHLYMQKERGGPSVVALGMTHRFLAKYVTVVLYKSLQR 301

[Close]

BLAST of Carg07887 vs. NCBI nr
Match: XP_023003375.1 (SNF1-related protein kinase regulatory subunit beta-2-like [Cucurbita maxima])

HSP 1 Score: 330.9 bits (847), Expect = 6.6e-87
Identity = 160/164 (97.56%), Postives = 164/164 (100.00%), Query Frame = 0


Query: 8   IRIPLQRSGKDFTIVKVLPSGVYQYRFIADGQWRYAPDLPWARDDSGNAYNILDLQDYVP 67
           ++IPLQRSGKDFTIVKVLPSGVYQYRFIADGQWRYAPDLPWA+DDSGNAYNILDLQDYVP
Sbjct: 137 MKIPLQRSGKDFTIVKVLPSGVYQYRFIADGQWRYAPDLPWAQDDSGNAYNILDLQDYVP 196

Query: 68  EDIESISSFEPPQSPESSYSSLQLGSEDFSKEPPLAPPHLKSTLLDMPCPYNEILPPLSR 127
           EDIESISSFEPPQSPESSYSSLQLGSEDFSKEPPLAPPHLKSTLLDMPCPYNEILPPLSR
Sbjct: 197 EDIESISSFEPPQSPESSYSSLQLGSEDFSKEPPLAPPHLKSTLLDMPCPYNEILPPLSR 256

Query: 128 PQHVMLNHLYMQKERGGPSVVALGMTHRFLAKYVTVVLYKSLQR 172
           PQHVMLNHLYMQKERGGPSVVALGMT+RFLAKYVTVVLYKSLQR
Sbjct: 257 PQHVMLNHLYMQKERGGPSVVALGMTNRFLAKYVTVVLYKSLQR 300

[Close]

BLAST of Carg07887 vs. NCBI nr
Match: XP_038882886.1 (SNF1-related protein kinase regulatory subunit beta-2 [Benincasa hispida])

HSP 1 Score: 322.0 bits (824), Expect = 3.1e-84
Identity = 152/164 (92.68%), Postives = 164/164 (100.00%), Query Frame = 0


Query: 8   IRIPLQRSGKDFTIVKVLPSGVYQYRFIADGQWRYAPDLPWARDDSGNAYNILDLQDYVP 67
           ++IPLQRSGKDFTI+KVLPSGVYQYRFIADGQWRYAPDLPWA+DD+GNAYNILDLQDYVP
Sbjct: 125 MKIPLQRSGKDFTIMKVLPSGVYQYRFIADGQWRYAPDLPWAQDDAGNAYNILDLQDYVP 184

Query: 68  EDIESISSFEPPQSPESSYSSLQLGSEDFSKEPPLAPPHLKSTLLDMPCPYNEILPPLSR 127
           EDIESISSFEPPQSPESSY+SLQLG++D+SKEPPLAPPHLK+TLLDMPCP+NEILPPLSR
Sbjct: 185 EDIESISSFEPPQSPESSYNSLQLGADDYSKEPPLAPPHLKTTLLDMPCPHNEILPPLSR 244

Query: 128 PQHVMLNHLYMQKERGGPSVVALGMTHRFLAKYVTVVLYKSLQR 172
           PQHV+LNHLYMQKERGGPSVVALGMTHRFLAKYVTVVLYKSLQR
Sbjct: 245 PQHVVLNHLYMQKERGGPSVVALGMTHRFLAKYVTVVLYKSLQR 288

[Close]

BLAST of Carg07887 vs. ExPASy Swiss-Prot
Match: Q9SCY5 (SNF1-related protein kinase regulatory subunit beta-2 OS=Arabidopsis thaliana OX=3702 GN=KINB2 PE=1 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 3.8e-69
Identity = 126/163 (77.30%), Postives = 144/163 (88.34%), Query Frame = 0


Query: 9   RIPLQRSGKDFTIVKVLPSGVYQYRFIADGQWRYAPDLPWARDDSGNAYNILDLQDYVPE 68
           R  LQRSGKDFTI+KVLPSGVY+YRFI DGQWR+AP+LP ARDD+GN +NILDLQDYVPE
Sbjct: 127 RSRLQRSGKDFTIMKVLPSGVYEYRFIVDGQWRHAPELPLARDDAGNTFNILDLQDYVPE 186

Query: 69  DIESISSFEPPQSPESSYSSLQLGSEDFSKEPPLAPPHLKSTLLDMPCPYNEILPPLSRP 128
           DI+SIS FEPPQSPE+SYS+L LG+ED+SKEPP+ PPHL+ TLL++P    +I  PL RP
Sbjct: 187 DIQSISGFEPPQSPENSYSNLLLGAEDYSKEPPVVPPHLQMTLLNLPAANPDIPSPLPRP 246

Query: 129 QHVMLNHLYMQKERGGPSVVALGMTHRFLAKYVTVVLYKSLQR 172
           QHV+LNHLYMQK + GPSVVALG THRFLAKYVTVVLYKSLQR
Sbjct: 247 QHVILNHLYMQKGKSGPSVVALGSTHRFLAKYVTVVLYKSLQR 289

[Close]

BLAST of Carg07887 vs. ExPASy Swiss-Prot
Match: Q84VQ1 (SNF1-related protein kinase regulatory subunit beta-1 OS=Arabidopsis thaliana OX=3702 GN=KINB1 PE=1 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 5.9e-38
Identity = 81/163 (49.69%), Postives = 110/163 (67.48%), Query Frame = 0


Query: 9   RIPLQRSGKDFTIVKVLPSGVYQYRFIADGQWRYAPDLPWARDDSGNAYNILDLQDYVPE 68
           R  LQ+SGKD +I+ VLPSG+Y Y+ I DG+ +Y PDLP+  D+ GN  NILD+ ++VPE
Sbjct: 125 RKKLQKSGKDHSILFVLPSGIYHYKVIVDGESKYIPDLPFVADEVGNVCNILDVHNFVPE 184

Query: 69  DIESISSFEPPQSPESSYSSLQLGSEDFSKEPPLAPPHLKSTLLDMPCPYNEILPPLSRP 128
           + ESI  FE P SP+ SY      +ED++KEP   PP L  TLL       E     ++P
Sbjct: 185 NPESIVEFEAPPSPDHSYGQTLPAAEDYAKEPLAVPPQLHLTLLGT----TEETAIATKP 244

Query: 129 QHVMLNHLYMQKERGGPSVVALGMTHRFLAKYVTVVLYKSLQR 172
           QHV+LNH+++++     S+VALG+THRF +KY+TVVLYK L R
Sbjct: 245 QHVVLNHVFIEQGWTPQSIVALGLTHRFESKYITVVLYKPLTR 283

[Close]

BLAST of Carg07887 vs. ExPASy Swiss-Prot
Match: O43741 (5'-AMP-activated protein kinase subunit beta-2 OS=Homo sapiens OX=9606 GN=PRKAB2 PE=1 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 4.0e-18
Identity = 60/173 (34.68%), Postives = 84/173 (48.55%), Query Frame = 0


Query: 9   RIPLQRSGKDFTIVKVLPSGVYQYRFIADGQWRYAPDLPWARDDSGNAYNILDLQ--DYV 68
           +IPL +S  DF  +  LP G +QY+F  DGQW + P  P      G   N++ ++  D+ 
Sbjct: 102 KIPLIKSHNDFVAILDLPEGEHQYKFFVDGQWVHDPSEPVVTSQLGTINNLIHVKKSDFE 161

Query: 69  PEDIESISSFEPPQSPESSYSSLQLG----------SEDFSKEPPLAPPHLKSTLLDMPC 128
             D   + S E  ++     SS   G          SE+  K PP+ PPHL   +L+   
Sbjct: 162 VFDALKLDSMESSETSCRDLSSSPPGPYGQEMYAFRSEERFKSPPILPPHLLQVILNKDT 221

Query: 129 PYNEILPPLSRPQHVMLNHLYMQKERGGPSVVALGMTHRFLAKYVTVVLYKSL 170
             +     L  P HVMLNHLY    +   SV+ L  THR+  KYVT +LYK +
Sbjct: 222 NISCDPALLPEPNHVMLNHLYALSIK--DSVMVLSATHRYKKKYVTTLLYKPI 272

[Close]

BLAST of Carg07887 vs. ExPASy Swiss-Prot
Match: Q9R078 (5'-AMP-activated protein kinase subunit beta-1 OS=Mus musculus OX=10090 GN=Prkab1 PE=1 SV=2)

HSP 1 Score: 92.0 bits (227), Expect = 6.8e-18
Identity = 59/174 (33.91%), Postives = 82/174 (47.13%), Query Frame = 0


Query: 9   RIPLQRSGKDFTIVKVLPSGVYQYRFIADGQWRYAPDLPWARDDSGNAYNILDLQDYVPE 68
           ++PL RS  +F  +  LP G +QY+F  DGQW + P  P      G   NI+ ++    E
Sbjct: 102 KLPLTRSQNNFVAILDLPEGEHQYKFFVDGQWTHDPSEPIVTSQLGTVNNIIQVKKTDFE 161

Query: 69  -------------DIESISSFEPPQSPESSYSSLQLGSEDFSKEPPLAPPHLKSTLLDMP 128
                        D+  +SS  P    +  Y S     E+  K PP+ PPHL   +L+  
Sbjct: 162 VFDALMVDSQKCSDVSELSSSPPGPYHQEPYMS---KPEERFKAPPILPPHLLQVILNKD 221

Query: 129 CPYNEILPPLSRPQHVMLNHLYMQKERGGPSVVALGMTHRFLAKYVTVVLYKSL 170
              +     L  P HVMLNHLY    + G  V+ L  THR+  KYVT +LYK +
Sbjct: 222 TGISCDPALLPEPNHVMLNHLYALSIKDG--VMVLSATHRYKKKYVTTLLYKPI 270

[Close]

BLAST of Carg07887 vs. ExPASy Swiss-Prot
Match: P80386 (5'-AMP-activated protein kinase subunit beta-1 OS=Rattus norvegicus OX=10116 GN=Prkab1 PE=1 SV=4)

HSP 1 Score: 91.7 bits (226), Expect = 8.9e-18
Identity = 59/174 (33.91%), Postives = 82/174 (47.13%), Query Frame = 0


Query: 9   RIPLQRSGKDFTIVKVLPSGVYQYRFIADGQWRYAPDLPWARDDSGNAYNILDLQDYVPE 68
           ++PL RS  +F  +  LP G +QY+F  DGQW + P  P      G   NI+ ++    E
Sbjct: 102 KLPLTRSQNNFVAILDLPEGEHQYKFFVDGQWTHDPSEPIVTSQLGTVNNIIQVKKTDFE 161

Query: 69  -------------DIESISSFEPPQSPESSYSSLQLGSEDFSKEPPLAPPHLKSTLLDMP 128
                        D+  +SS  P    +  Y S     E+  K PP+ PPHL   +L+  
Sbjct: 162 VFDALMVDSQKCSDVSELSSSPPGPYHQEPYIS---KPEERFKAPPILPPHLLQVILNKD 221

Query: 129 CPYNEILPPLSRPQHVMLNHLYMQKERGGPSVVALGMTHRFLAKYVTVVLYKSL 170
              +     L  P HVMLNHLY    + G  V+ L  THR+  KYVT +LYK +
Sbjct: 222 TGISCDPALLPEPNHVMLNHLYALSIKDG--VMVLSATHRYKKKYVTTLLYKPI 270

[Close]

BLAST of Carg07887 vs. ExPASy TrEMBL
Match: A0A6J1EM37 (SNF1-related protein kinase regulatory subunit beta-2-like OS=Cucurbita moschata OX=3662 GN=LOC111433799 PE=3 SV=1)

HSP 1 Score: 333.6 bits (854), Expect = 5.0e-88
Identity = 161/164 (98.17%), Postives = 164/164 (100.00%), Query Frame = 0


Query: 8   IRIPLQRSGKDFTIVKVLPSGVYQYRFIADGQWRYAPDLPWARDDSGNAYNILDLQDYVP 67
           ++IPLQRSGKDFTIVKVLPSGVYQYRFIADGQWRYAPDLPWA+DDSGNAYNILDLQDYVP
Sbjct: 138 MKIPLQRSGKDFTIVKVLPSGVYQYRFIADGQWRYAPDLPWAQDDSGNAYNILDLQDYVP 197

Query: 68  EDIESISSFEPPQSPESSYSSLQLGSEDFSKEPPLAPPHLKSTLLDMPCPYNEILPPLSR 127
           EDIESISSFEPPQSPESSYSSLQLGSEDFSKEPPLAPPHLKSTLLDMPCPYNEILPPLSR
Sbjct: 198 EDIESISSFEPPQSPESSYSSLQLGSEDFSKEPPLAPPHLKSTLLDMPCPYNEILPPLSR 257

Query: 128 PQHVMLNHLYMQKERGGPSVVALGMTHRFLAKYVTVVLYKSLQR 172
           PQHVMLNHLYMQKERGGPSVVALGMTHRFLAKYVTVVLYKSLQR
Sbjct: 258 PQHVMLNHLYMQKERGGPSVVALGMTHRFLAKYVTVVLYKSLQR 301

[Close]

BLAST of Carg07887 vs. ExPASy TrEMBL
Match: A0A6J1KRL1 (SNF1-related protein kinase regulatory subunit beta-2-like OS=Cucurbita maxima OX=3661 GN=LOC111497000 PE=3 SV=1)

HSP 1 Score: 330.9 bits (847), Expect = 3.2e-87
Identity = 160/164 (97.56%), Postives = 164/164 (100.00%), Query Frame = 0


Query: 8   IRIPLQRSGKDFTIVKVLPSGVYQYRFIADGQWRYAPDLPWARDDSGNAYNILDLQDYVP 67
           ++IPLQRSGKDFTIVKVLPSGVYQYRFIADGQWRYAPDLPWA+DDSGNAYNILDLQDYVP
Sbjct: 137 MKIPLQRSGKDFTIVKVLPSGVYQYRFIADGQWRYAPDLPWAQDDSGNAYNILDLQDYVP 196

Query: 68  EDIESISSFEPPQSPESSYSSLQLGSEDFSKEPPLAPPHLKSTLLDMPCPYNEILPPLSR 127
           EDIESISSFEPPQSPESSYSSLQLGSEDFSKEPPLAPPHLKSTLLDMPCPYNEILPPLSR
Sbjct: 197 EDIESISSFEPPQSPESSYSSLQLGSEDFSKEPPLAPPHLKSTLLDMPCPYNEILPPLSR 256

Query: 128 PQHVMLNHLYMQKERGGPSVVALGMTHRFLAKYVTVVLYKSLQR 172
           PQHVMLNHLYMQKERGGPSVVALGMT+RFLAKYVTVVLYKSLQR
Sbjct: 257 PQHVMLNHLYMQKERGGPSVVALGMTNRFLAKYVTVVLYKSLQR 300

[Close]

BLAST of Carg07887 vs. ExPASy TrEMBL
Match: A0A6J1GE75 (SNF1-related protein kinase regulatory subunit beta-2-like OS=Cucurbita moschata OX=3662 GN=LOC111453144 PE=3 SV=1)

HSP 1 Score: 320.5 bits (820), Expect = 4.3e-84
Identity = 154/163 (94.48%), Postives = 161/163 (98.77%), Query Frame = 0


Query: 9   RIPLQRSGKDFTIVKVLPSGVYQYRFIADGQWRYAPDLPWARDDSGNAYNILDLQDYVPE 68
           +IPLQRSGKDFTI+KVL SGVYQYRFIADGQWRYAPDLPWA+DD+GNAYNILDLQDYVPE
Sbjct: 135 KIPLQRSGKDFTIMKVLSSGVYQYRFIADGQWRYAPDLPWAQDDAGNAYNILDLQDYVPE 194

Query: 69  DIESISSFEPPQSPESSYSSLQLGSEDFSKEPPLAPPHLKSTLLDMPCPYNEILPPLSRP 128
           DIESISSFEPPQSPESSYSSLQLGSEDFSKEPPLAPPHLK+TLLDMP PYNEILPP+SRP
Sbjct: 195 DIESISSFEPPQSPESSYSSLQLGSEDFSKEPPLAPPHLKTTLLDMPSPYNEILPPISRP 254

Query: 129 QHVMLNHLYMQKERGGPSVVALGMTHRFLAKYVTVVLYKSLQR 172
           QHV+LNHLYMQKERGGPSVVALGMTHRFLAKYVTVVLYKSLQR
Sbjct: 255 QHVVLNHLYMQKERGGPSVVALGMTHRFLAKYVTVVLYKSLQR 297

[Close]

BLAST of Carg07887 vs. ExPASy TrEMBL
Match: A0A0A0KH57 (AMPKBI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G511020 PE=3 SV=1)

HSP 1 Score: 319.7 bits (818), Expect = 7.4e-84
Identity = 151/164 (92.07%), Postives = 163/164 (99.39%), Query Frame = 0


Query: 8   IRIPLQRSGKDFTIVKVLPSGVYQYRFIADGQWRYAPDLPWARDDSGNAYNILDLQDYVP 67
           ++IPLQRSGKDFTI+KVLPSGVYQYRFIADGQWRYAPDLPWA+DD+GNAYNILDLQDYVP
Sbjct: 134 MKIPLQRSGKDFTIMKVLPSGVYQYRFIADGQWRYAPDLPWAQDDAGNAYNILDLQDYVP 193

Query: 68  EDIESISSFEPPQSPESSYSSLQLGSEDFSKEPPLAPPHLKSTLLDMPCPYNEILPPLSR 127
           EDIESISSFEPPQSPESSY+SLQL ++D+SKEPPLAPPHLK+TLLDMPCPYNEILPP+SR
Sbjct: 194 EDIESISSFEPPQSPESSYNSLQLVADDYSKEPPLAPPHLKTTLLDMPCPYNEILPPISR 253

Query: 128 PQHVMLNHLYMQKERGGPSVVALGMTHRFLAKYVTVVLYKSLQR 172
           PQHV+LNHLYMQKERGGPSVVALGMTHRFLAKYVTVVLYKSLQR
Sbjct: 254 PQHVVLNHLYMQKERGGPSVVALGMTHRFLAKYVTVVLYKSLQR 297

[Close]

BLAST of Carg07887 vs. ExPASy TrEMBL
Match: A0A6J1IJ92 (SNF1-related protein kinase regulatory subunit beta-2-like OS=Cucurbita maxima OX=3661 GN=LOC111477935 PE=3 SV=1)

HSP 1 Score: 319.3 bits (817), Expect = 9.7e-84
Identity = 154/163 (94.48%), Postives = 160/163 (98.16%), Query Frame = 0


Query: 9   RIPLQRSGKDFTIVKVLPSGVYQYRFIADGQWRYAPDLPWARDDSGNAYNILDLQDYVPE 68
           +IPLQRSGKDFTI+KVL SGVYQYRFIADGQWRYAPDLPWA DD+GNAYNILDLQDYVPE
Sbjct: 135 KIPLQRSGKDFTIMKVLSSGVYQYRFIADGQWRYAPDLPWAPDDAGNAYNILDLQDYVPE 194

Query: 69  DIESISSFEPPQSPESSYSSLQLGSEDFSKEPPLAPPHLKSTLLDMPCPYNEILPPLSRP 128
           DIESISSFEPPQSPESSYSSLQLGSEDFSKEPPLAPPHLK+TLLDMP PYNEILPP+SRP
Sbjct: 195 DIESISSFEPPQSPESSYSSLQLGSEDFSKEPPLAPPHLKTTLLDMPSPYNEILPPISRP 254

Query: 129 QHVMLNHLYMQKERGGPSVVALGMTHRFLAKYVTVVLYKSLQR 172
           QHV+LNHLYMQKERGGPSVVALGMTHRFLAKYVTVVLYKSLQR
Sbjct: 255 QHVVLNHLYMQKERGGPSVVALGMTHRFLAKYVTVVLYKSLQR 297

[Close]

BLAST of Carg07887 vs. TAIR 10
Match: AT4G16360.1 (5'-AMP-activated protein kinase beta-2 subunit protein )

HSP 1 Score: 262.3 bits (669), Expect = 2.7e-70
Identity = 126/163 (77.30%), Postives = 144/163 (88.34%), Query Frame = 0


Query: 9   RIPLQRSGKDFTIVKVLPSGVYQYRFIADGQWRYAPDLPWARDDSGNAYNILDLQDYVPE 68
           R  LQRSGKDFTI+KVLPSGVY+YRFI DGQWR+AP+LP ARDD+GN +NILDLQDYVPE
Sbjct: 97  RSRLQRSGKDFTIMKVLPSGVYEYRFIVDGQWRHAPELPLARDDAGNTFNILDLQDYVPE 156

Query: 69  DIESISSFEPPQSPESSYSSLQLGSEDFSKEPPLAPPHLKSTLLDMPCPYNEILPPLSRP 128
           DI+SIS FEPPQSPE+SYS+L LG+ED+SKEPP+ PPHL+ TLL++P    +I  PL RP
Sbjct: 157 DIQSISGFEPPQSPENSYSNLLLGAEDYSKEPPVVPPHLQMTLLNLPAANPDIPSPLPRP 216

Query: 129 QHVMLNHLYMQKERGGPSVVALGMTHRFLAKYVTVVLYKSLQR 172
           QHV+LNHLYMQK + GPSVVALG THRFLAKYVTVVLYKSLQR
Sbjct: 217 QHVILNHLYMQKGKSGPSVVALGSTHRFLAKYVTVVLYKSLQR 259

[Close]

BLAST of Carg07887 vs. TAIR 10
Match: AT4G16360.3 (5'-AMP-activated protein kinase beta-2 subunit protein )

HSP 1 Score: 262.3 bits (669), Expect = 2.7e-70
Identity = 126/163 (77.30%), Postives = 144/163 (88.34%), Query Frame = 0


Query: 9   RIPLQRSGKDFTIVKVLPSGVYQYRFIADGQWRYAPDLPWARDDSGNAYNILDLQDYVPE 68
           R  LQRSGKDFTI+KVLPSGVY+YRFI DGQWR+AP+LP ARDD+GN +NILDLQDYVPE
Sbjct: 99  RSRLQRSGKDFTIMKVLPSGVYEYRFIVDGQWRHAPELPLARDDAGNTFNILDLQDYVPE 158

Query: 69  DIESISSFEPPQSPESSYSSLQLGSEDFSKEPPLAPPHLKSTLLDMPCPYNEILPPLSRP 128
           DI+SIS FEPPQSPE+SYS+L LG+ED+SKEPP+ PPHL+ TLL++P    +I  PL RP
Sbjct: 159 DIQSISGFEPPQSPENSYSNLLLGAEDYSKEPPVVPPHLQMTLLNLPAANPDIPSPLPRP 218

Query: 129 QHVMLNHLYMQKERGGPSVVALGMTHRFLAKYVTVVLYKSLQR 172
           QHV+LNHLYMQK + GPSVVALG THRFLAKYVTVVLYKSLQR
Sbjct: 219 QHVILNHLYMQKGKSGPSVVALGSTHRFLAKYVTVVLYKSLQR 261

[Close]

BLAST of Carg07887 vs. TAIR 10
Match: AT4G16360.2 (5'-AMP-activated protein kinase beta-2 subunit protein )

HSP 1 Score: 261.9 bits (668), Expect = 3.5e-70
Identity = 125/160 (78.12%), Postives = 143/160 (89.38%), Query Frame = 0


Query: 12  LQRSGKDFTIVKVLPSGVYQYRFIADGQWRYAPDLPWARDDSGNAYNILDLQDYVPEDIE 71
           LQRSGKDFTI+KVLPSGVY+YRFI DGQWR+AP+LP ARDD+GN +NILDLQDYVPEDI+
Sbjct: 99  LQRSGKDFTIMKVLPSGVYEYRFIVDGQWRHAPELPLARDDAGNTFNILDLQDYVPEDIQ 158

Query: 72  SISSFEPPQSPESSYSSLQLGSEDFSKEPPLAPPHLKSTLLDMPCPYNEILPPLSRPQHV 131
           SIS FEPPQSPE+SYS+L LG+ED+SKEPP+ PPHL+ TLL++P    +I  PL RPQHV
Sbjct: 159 SISGFEPPQSPENSYSNLLLGAEDYSKEPPVVPPHLQMTLLNLPAANPDIPSPLPRPQHV 218

Query: 132 MLNHLYMQKERGGPSVVALGMTHRFLAKYVTVVLYKSLQR 172
           +LNHLYMQK + GPSVVALG THRFLAKYVTVVLYKSLQR
Sbjct: 219 ILNHLYMQKGKSGPSVVALGSTHRFLAKYVTVVLYKSLQR 258

[Close]

BLAST of Carg07887 vs. TAIR 10
Match: AT5G21170.1 (5'-AMP-activated protein kinase beta-2 subunit protein )

HSP 1 Score: 158.7 bits (400), Expect = 4.2e-39
Identity = 81/163 (49.69%), Postives = 110/163 (67.48%), Query Frame = 0


Query: 9   RIPLQRSGKDFTIVKVLPSGVYQYRFIADGQWRYAPDLPWARDDSGNAYNILDLQDYVPE 68
           R  LQ+SGKD +I+ VLPSG+Y Y+ I DG+ +Y PDLP+  D+ GN  NILD+ ++VPE
Sbjct: 125 RKKLQKSGKDHSILFVLPSGIYHYKVIVDGESKYIPDLPFVADEVGNVCNILDVHNFVPE 184

Query: 69  DIESISSFEPPQSPESSYSSLQLGSEDFSKEPPLAPPHLKSTLLDMPCPYNEILPPLSRP 128
           + ESI  FE P SP+ SY      +ED++KEP   PP L  TLL       E     ++P
Sbjct: 185 NPESIVEFEAPPSPDHSYGQTLPAAEDYAKEPLAVPPQLHLTLLGT----TEETAIATKP 244

Query: 129 QHVMLNHLYMQKERGGPSVVALGMTHRFLAKYVTVVLYKSLQR 172
           QHV+LNH+++++     S+VALG+THRF +KY+TVVLYK L R
Sbjct: 245 QHVVLNHVFIEQGWTPQSIVALGLTHRFESKYITVVLYKPLTR 283

[Close]

BLAST of Carg07887 vs. TAIR 10
Match: AT5G21170.2 (5'-AMP-activated protein kinase beta-2 subunit protein )

HSP 1 Score: 158.7 bits (400), Expect = 4.2e-39
Identity = 81/163 (49.69%), Postives = 110/163 (67.48%), Query Frame = 0


Query: 9   RIPLQRSGKDFTIVKVLPSGVYQYRFIADGQWRYAPDLPWARDDSGNAYNILDLQDYVPE 68
           R  LQ+SGKD +I+ VLPSG+Y Y+ I DG+ +Y PDLP+  D+ GN  NILD+ ++VPE
Sbjct: 162 RKKLQKSGKDHSILFVLPSGIYHYKVIVDGESKYIPDLPFVADEVGNVCNILDVHNFVPE 221

Query: 69  DIESISSFEPPQSPESSYSSLQLGSEDFSKEPPLAPPHLKSTLLDMPCPYNEILPPLSRP 128
           + ESI  FE P SP+ SY      +ED++KEP   PP L  TLL       E     ++P
Sbjct: 222 NPESIVEFEAPPSPDHSYGQTLPAAEDYAKEPLAVPPQLHLTLLGT----TEETAIATKP 281

Query: 129 QHVMLNHLYMQKERGGPSVVALGMTHRFLAKYVTVVLYKSLQR 172
           QHV+LNH+++++     S+VALG+THRF +KY+TVVLYK L R
Sbjct: 282 QHVVLNHVFIEQGWTPQSIVALGLTHRFESKYITVVLYKPLTR 320

The following BLAST results are available for this feature:

BLAST of Carg07887 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7026674.1	8.1e-93	100.00	SNF1-related protein kinase regulatory subunit beta-2, partial [Cucurbita argyro...	[more]
XP_022926790.1	1.0e-87	98.17	SNF1-related protein kinase regulatory subunit beta-2-like [Cucurbita moschata]	[more]
XP_023517360.1	1.0e-87	98.17	SNF1-related protein kinase regulatory subunit beta-2-like [Cucurbita pepo subsp...	[more]
XP_023003375.1	6.6e-87	97.56	SNF1-related protein kinase regulatory subunit beta-2-like [Cucurbita maxima]	[more]
XP_038882886.1	3.1e-84	92.68	SNF1-related protein kinase regulatory subunit beta-2 [Benincasa hispida]	[more]

BLAST of Carg07887 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SCY5	3.8e-69	77.30	SNF1-related protein kinase regulatory subunit beta-2 OS=Arabidopsis thaliana OX...	[more]
Q84VQ1	5.9e-38	49.69	SNF1-related protein kinase regulatory subunit beta-1 OS=Arabidopsis thaliana OX...	[more]
O43741	4.0e-18	34.68	5'-AMP-activated protein kinase subunit beta-2 OS=Homo sapiens OX=9606 GN=PRKAB2...	[more]
Q9R078	6.8e-18	33.91	5'-AMP-activated protein kinase subunit beta-1 OS=Mus musculus OX=10090 GN=Prkab...	[more]
P80386	8.9e-18	33.91	5'-AMP-activated protein kinase subunit beta-1 OS=Rattus norvegicus OX=10116 GN=...	[more]

BLAST of Carg07887 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EM37	5.0e-88	98.17	SNF1-related protein kinase regulatory subunit beta-2-like OS=Cucurbita moschata...	[more]
A0A6J1KRL1	3.2e-87	97.56	SNF1-related protein kinase regulatory subunit beta-2-like OS=Cucurbita maxima O...	[more]
A0A6J1GE75	4.3e-84	94.48	SNF1-related protein kinase regulatory subunit beta-2-like OS=Cucurbita moschata...	[more]
A0A0A0KH57	7.4e-84	92.07	AMPKBI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G511020 PE=3...	[more]
A0A6J1IJ92	9.7e-84	94.48	SNF1-related protein kinase regulatory subunit beta-2-like OS=Cucurbita maxima O...	[more]

BLAST of Carg07887 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G16360.1	2.7e-70	77.30	5'-AMP-activated protein kinase beta-2 subunit protein	[more]
AT4G16360.3	2.7e-70	77.30	5'-AMP-activated protein kinase beta-2 subunit protein	[more]
AT4G16360.2	3.5e-70	78.13	5'-AMP-activated protein kinase beta-2 subunit protein	[more]
AT5G21170.1	4.2e-39	49.69	5'-AMP-activated protein kinase beta-2 subunit protein	[more]
AT5G21170.2	4.2e-39	49.69	5'-AMP-activated protein kinase beta-2 subunit protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006828	Association with the SNF1 complex (ASC) domain	SMART	SM01010	AMPKBI_2	coord: 79..169 e-value: 4.9E-21 score: 85.9
IPR006828	Association with the SNF1 complex (ASC) domain	PFAM	PF04739	AMPKBI	coord: 97..167 e-value: 2.7E-18 score: 66.0
None	No IPR available	GENE3D	6.20.250.60		coord: 71..169 e-value: 5.1E-23 score: 83.0
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 74..94
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 73..98
None	No IPR available	PANTHER	PTHR46316:SF2	SNF1-RELATED PROTEIN KINASE REGULATORY SUBUNIT BETA-2	coord: 8..171
None	No IPR available	CDD	cd02859	E_set_AMPKbeta_like_N	coord: 9..61 e-value: 3.31708E-17 score: 70.3204
IPR032640	AMP-activated protein kinase, glycogen-binding domain	PFAM	PF16561	AMPK1_CBM	coord: 9..63 e-value: 5.4E-17 score: 61.8
IPR013783	Immunoglobulin-like fold	GENE3D	2.60.40.10	Immunoglobulins	coord: 7..66 e-value: 2.8E-13 score: 51.5
IPR043554	SNF1-related protein kinase regulatory subunit beta	PANTHER	PTHR46316	SNF1-RELATED PROTEIN KINASE REGULATORY SUBUNIT BETA-1	coord: 8..171
IPR014756	Immunoglobulin E-set	SUPERFAMILY	81296	E set domains	coord: 9..69
IPR037256	ASC domain superfamily	SUPERFAMILY	160219	AMPKBI-like	coord: 66..168

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg07887-RA	Carg07887-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016310	phosphorylation
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0016301	kinase activity
molecular_function	GO:0005515	protein binding