Carg07886 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg07886 vs. NCBI nr
Match: KAG7026673.1 (Heavy metal-associated isoprenylated plant protein 8 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 516.5 bits (1329), Expect = 1.4e-142
Identity = 287/287 (100.00%), Postives = 287/287 (100.00%), Query Frame = 0

Query: 1   MGEEIKKEGEDQKKEETKQEQEEPTESAAAATEEEKKQDSKKNEEEPPSEIVLKVDMHCE 60
           MGEEIKKEGEDQKKEETKQEQEEPTESAAAATEEEKKQDSKKNEEEPPSEIVLKVDMHCE
Sbjct: 1   MGEEIKKEGEDQKKEETKQEQEEPTESAAAATEEEKKQDSKKNEEEPPSEIVLKVDMHCE 60

Query: 61  ACARKVARSLRGFQGVETVTTASRAGKVVVKGKEVDPLKVCERLQKKSGRKVELISPLPK 120
           ACARKVARSLRGFQGVETVTTASRAGKVVVKGKEVDPLKVCERLQKKSGRKVELISPLPK
Sbjct: 61  ACARKVARSLRGFQGVETVTTASRAGKVVVKGKEVDPLKVCERLQKKSGRKVELISPLPK 120

Query: 121 PPEQQPKEDEKQPKEEKKEEATPPPPAVVTVVLQVQMHCEACAQGLRKRIRKIKGVESVE 180
           PPEQQPKEDEKQPKEEKKEEATPPPPAVVTVVLQVQMHCEACAQGLRKRIRKIKGVESVE
Sbjct: 121 PPEQQPKEDEKQPKEEKKEEATPPPPAVVTVVLQVQMHCEACAQGLRKRIRKIKGVESVE 180

Query: 181 TEPSNNRVIVKGVMDATTLVNEVSKRTRKPASIVVKEEEKKEEEKKEEEKPAAEEKPEEK 240
           TEPSNNRVIVKGVMDATTLVNEVSKRTRKPASIVVKEEEKKEEEKKEEEKPAAEEKPEEK
Sbjct: 181 TEPSNNRVIVKGVMDATTLVNEVSKRTRKPASIVVKEEEKKEEEKKEEEKPAAEEKPEEK 240

Query: 241 KETQEEDDEKFDIKRFEHWPTKHYTEYAYYYPERIFSDENPNGCSIM 288
           KETQEEDDEKFDIKRFEHWPTKHYTEYAYYYPERIFSDENPNGCSIM
Sbjct: 241 KETQEEDDEKFDIKRFEHWPTKHYTEYAYYYPERIFSDENPNGCSIM 287

BLAST of Carg07886 vs. NCBI nr
Match: KAG6594708.1 (Heavy metal-associated isoprenylated plant protein 8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 509.2 bits (1310), Expect = 2.3e-140
Identity = 283/287 (98.61%), Postives = 285/287 (99.30%), Query Frame = 0

Query: 1   MGEEIKKEGEDQKKEETKQEQEEPTESAAAATEEEKKQDSKKNEEEPPSEIVLKVDMHCE 60
           MGEEIKKEGEDQKKEETKQEQEEPTESAAAATEEEKKQDSKKNEEEPPSEIVLKVDMHCE
Sbjct: 1   MGEEIKKEGEDQKKEETKQEQEEPTESAAAATEEEKKQDSKKNEEEPPSEIVLKVDMHCE 60

Query: 61  ACARKVARSLRGFQGVETVTTASRAGKVVVKGKEVDPLKVCERLQKKSGRKVELISPLPK 120
           ACARKVAR+LRGFQGVETVTT SRAGKVVVKGKEVDPLKVCERLQKKSGRKVELISPLPK
Sbjct: 61  ACARKVARALRGFQGVETVTTDSRAGKVVVKGKEVDPLKVCERLQKKSGRKVELISPLPK 120

Query: 121 PPEQQPKEDEKQPKEEKKEEATPPPPAVVTVVLQVQMHCEACAQGLRKRIRKIKGVESVE 180
           PPEQQPKEDEKQPKEEKKEEATPPPPAVVTVVLQVQMHCEACAQGLRKRIRKIKGVESVE
Sbjct: 121 PPEQQPKEDEKQPKEEKKEEATPPPPAVVTVVLQVQMHCEACAQGLRKRIRKIKGVESVE 180

Query: 181 TEPSNNRVIVKGVMDATTLVNEVSKRTRKPASIVVKEEEKKEEEKKEEEKPAAEEKPEEK 240
           TEPSNNRVIVKGVMDAT LVNEVSKRTRKPASIVVKEEEKKEEEKKEEEKPAAEEKPEEK
Sbjct: 181 TEPSNNRVIVKGVMDATMLVNEVSKRTRKPASIVVKEEEKKEEEKKEEEKPAAEEKPEEK 240

Query: 241 KETQEEDDEKFDIKRFEHWPTKHYTEYAYYYPERIFSDENPNGCSIM 288
           KETQEEDD+KFDIKRFEHWPTKHYTEYAYYYPERIFSDENPNGCSIM
Sbjct: 241 KETQEEDDKKFDIKRFEHWPTKHYTEYAYYYPERIFSDENPNGCSIM 287

BLAST of Carg07886 vs. NCBI nr
Match: XP_022926792.1 (heavy metal-associated isoprenylated plant protein 7-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 505.8 bits (1301), Expect = 2.5e-139
Identity = 282/287 (98.26%), Postives = 284/287 (98.95%), Query Frame = 0

Query: 1   MGEEIKKEGEDQKKEETKQEQEEPTESAAAATEEEKKQDSKKNEEEPPSEIVLKVDMHCE 60
           MGEEIKKEGEDQKKEETKQEQEEPTESAAAATEEEKKQDSKKNEEE PSEIVLKVDMHCE
Sbjct: 1   MGEEIKKEGEDQKKEETKQEQEEPTESAAAATEEEKKQDSKKNEEESPSEIVLKVDMHCE 60

Query: 61  ACARKVARSLRGFQGVETVTTASRAGKVVVKGKEVDPLKVCERLQKKSGRKVELISPLPK 120
           ACARKVAR+LRGFQGVETVTT SRAGKVVVKGKEVDPLKVCERLQKKSGRKVELISPLPK
Sbjct: 61  ACARKVARALRGFQGVETVTTDSRAGKVVVKGKEVDPLKVCERLQKKSGRKVELISPLPK 120

Query: 121 PPEQQPKEDEKQPKEEKKEEATPPPPAVVTVVLQVQMHCEACAQGLRKRIRKIKGVESVE 180
           PPEQQPKEDEKQPKEEKKEEATPPPPAVVTVVLQVQMHCEACAQGLRKRIRKIKGVESVE
Sbjct: 121 PPEQQPKEDEKQPKEEKKEEATPPPPAVVTVVLQVQMHCEACAQGLRKRIRKIKGVESVE 180

Query: 181 TEPSNNRVIVKGVMDATTLVNEVSKRTRKPASIVVKEEEKKEEEKKEEEKPAAEEKPEEK 240
           TEPSNNRVIVKGVMDATTLVNEVSKRTRKPASIVVKEEEKKEEEKKE EKPAAEEKPEEK
Sbjct: 181 TEPSNNRVIVKGVMDATTLVNEVSKRTRKPASIVVKEEEKKEEEKKEVEKPAAEEKPEEK 240

Query: 241 KETQEEDDEKFDIKRFEHWPTKHYTEYAYYYPERIFSDENPNGCSIM 288
           KETQEEDD+KFDIKRFEHWPTKHYTEYAYYYPERIFSDENPNGCSIM
Sbjct: 241 KETQEEDDKKFDIKRFEHWPTKHYTEYAYYYPERIFSDENPNGCSIM 287

BLAST of Carg07886 vs. NCBI nr
Match: XP_022926793.1 (heavy metal-associated isoprenylated plant protein 7-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 499.6 bits (1285), Expect = 1.8e-137
Identity = 281/287 (97.91%), Postives = 283/287 (98.61%), Query Frame = 0

Query: 1   MGEEIKKEGEDQKKEETKQEQEEPTESAAAATEEEKKQDSKKNEEEPPSEIVLKVDMHCE 60
           MGEEIKKEGEDQKKEETKQEQEEPTESAAAATEEEKKQDSKKNEEE PSEIVLKVDMHCE
Sbjct: 1   MGEEIKKEGEDQKKEETKQEQEEPTESAAAATEEEKKQDSKKNEEESPSEIVLKVDMHCE 60

Query: 61  ACARKVARSLRGFQGVETVTTASRAGKVVVKGKEVDPLKVCERLQKKSGRKVELISPLPK 120
           ACARKVAR+LRGFQGVETVTT SRAGKVVVKGKEVDPLKVCERLQKKSGRKVELISPLPK
Sbjct: 61  ACARKVARALRGFQGVETVTTDSRAGKVVVKGKEVDPLKVCERLQKKSGRKVELISPLPK 120

Query: 121 PPEQQPKEDEKQPKEEKKEEATPPPPAVVTVVLQVQMHCEACAQGLRKRIRKIKGVESVE 180
           PPEQQPKEDEKQPKEEKK EATPPPPAVVTVVLQVQMHCEACAQGLRKRIRKIKGVESVE
Sbjct: 121 PPEQQPKEDEKQPKEEKK-EATPPPPAVVTVVLQVQMHCEACAQGLRKRIRKIKGVESVE 180

Query: 181 TEPSNNRVIVKGVMDATTLVNEVSKRTRKPASIVVKEEEKKEEEKKEEEKPAAEEKPEEK 240
           TEPSNNRVIVKGVMDATTLVNEVSKRTRKPASIVVKEEEKKEEEKKE EKPAAEEKPEEK
Sbjct: 181 TEPSNNRVIVKGVMDATTLVNEVSKRTRKPASIVVKEEEKKEEEKKEVEKPAAEEKPEEK 240

Query: 241 KETQEEDDEKFDIKRFEHWPTKHYTEYAYYYPERIFSDENPNGCSIM 288
           KETQEEDD+KFDIKRFEHWPTKHYTEYAYYYPERIFSDENPNGCSIM
Sbjct: 241 KETQEEDDKKFDIKRFEHWPTKHYTEYAYYYPERIFSDENPNGCSIM 286

BLAST of Carg07886 vs. NCBI nr
Match: XP_023517116.1 (heavy metal-associated isoprenylated plant protein 7-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 485.7 bits (1249), Expect = 2.7e-133
Identity = 277/292 (94.86%), Postives = 281/292 (96.23%), Query Frame = 0

Query: 1   MGEEIKK--EGEDQKKEETKQEQEEPTES---AAAATEEEKKQDSKKNEEEPPSEIVLKV 60
           MGEEIK+  EGEDQKKEETKQE+EEPTES   AAAATEEEKKQDSKKNEEEPPSEIVLKV
Sbjct: 1   MGEEIKRKEEGEDQKKEETKQEKEEPTESSAEAAAATEEEKKQDSKKNEEEPPSEIVLKV 60

Query: 61  DMHCEACARKVARSLRGFQGVETVTTASRAGKVVVKGKEVDPLKVCERLQKKSGRKVELI 120
           DMHCEACARKVAR+LRGFQGVE VTT SRAGKVVVKGKE DPLKVCERLQKKSGRKVELI
Sbjct: 61  DMHCEACARKVARALRGFQGVENVTTDSRAGKVVVKGKEADPLKVCERLQKKSGRKVELI 120

Query: 121 SPLPKPPEQQPKEDEKQPKEEKKEEATPPPPAVVTVVLQVQMHCEACAQGLRKRIRKIKG 180
           SPLPK PEQQPKEDEKQPKEEKKEEATPPPPAVVTVVLQVQMHCEACAQGLRKRIRKIKG
Sbjct: 121 SPLPKLPEQQPKEDEKQPKEEKKEEATPPPPAVVTVVLQVQMHCEACAQGLRKRIRKIKG 180

Query: 181 VESVETEPSNNRVIVKGVMDATTLVNEVSKRTRKPASIVVKEEEKKEEEKKEEEKPAAEE 240
           VESVETE SNNRVIVKGVMDATTLVNEVSKRTRKPASIVVKEEEKKEEEKKEEEKPA EE
Sbjct: 181 VESVETELSNNRVIVKGVMDATTLVNEVSKRTRKPASIVVKEEEKKEEEKKEEEKPATEE 240

Query: 241 KPEEKKETQEEDDEKFDIKRFEHWPTKHYTEYAYYYPERIFSDENPNGCSIM 288
           KPEEKKETQEEDD+KFDIKRFEHWPTKHYTEYAYYYPERIFSDENPNGCSIM
Sbjct: 241 KPEEKKETQEEDDKKFDIKRFEHWPTKHYTEYAYYYPERIFSDENPNGCSIM 292

BLAST of Carg07886 vs. ExPASy Swiss-Prot
Match: Q9C5D3 (Heavy metal-associated isoprenylated plant protein 7 OS=Arabidopsis thaliana OX=3702 GN=HIPP07 PE=1 SV=1)

HSP 1 Score: 167.5 bits (423), Expect = 2.1e-40
Identity = 143/343 (41.69%), Postives = 193/343 (56.27%), Query Frame = 0

Query: 4   EIKKEGEDQKKEETKQEQEEPTESAAAATEEEKKQDSKKNEEE------PPSEIVLKVDM 63
           E KKEGED+K +  K+ ++   +     + ++ K+DS     E      PP E+VLKV M
Sbjct: 22  EEKKEGEDKKVDAEKKGEDSDKKPQEGESNKDSKEDSAPAAPEAPAPPPPPQEVVLKVYM 81

Query: 64  HCEACARKVARSLRGFQGVETVTTASRAGKVVVKGKEVDPLKVCERLQKKSGRKVELISP 123
           HCE CARKV R L+GF+GVE V T  + GKVVVKG++ DPLKV  R+Q+K+ R+V+L+SP
Sbjct: 82  HCEGCARKVRRCLKGFEGVEDVMTDCKTGKVVVKGEKADPLKVLARVQRKTHRQVQLLSP 141

Query: 124 LPKPPEQQPK--EDEKQPKEEKKEEATPPPPAVVTVVLQVQMHCEACAQGLRKRIRKIKG 183
           +P PP    K  E++K   EEKK E    PP VVTVVL+V MHCEACA  ++KRI ++KG
Sbjct: 142 IPPPPPPPEKKAEEDKPIVEEKKVE----PPVVVTVVLKVHMHCEACATEIKKRIMRMKG 201

Query: 184 VESVETEPSNNRVIVKGVMDATTLVNEVSKRTRKPASIV---------------VKEEEK 243
           VES E++  +++V VKGV +   LV  V KRT K A+I+                 E EK
Sbjct: 202 VESAESDLKSSQVTVKGVFEPQKLVEYVYKRTGKHAAIMKIDPPPPPPPEEAAAAAEGEK 261

Query: 244 KEEEKKEEEKPAAEEK------PEEKKETQ--------------EEDDEKFDIKRFEHWP 288
           KEEEK E E    E K       EEKKE                EE+ +  ++++ E+  
Sbjct: 262 KEEEKGEGESKGEEGKDDKAKTDEEKKEGDGGKGEGEAADNGGGEEEGKVVEVRKIEN-- 321

BLAST of Carg07886 vs. ExPASy Swiss-Prot
Match: Q9M8T7 (Heavy metal-associated isoprenylated plant protein 8 OS=Arabidopsis thaliana OX=3702 GN=HIPP08 PE=3 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 1.3e-29
Identity = 109/282 (38.65%), Postives = 158/282 (56.03%), Query Frame = 0

Query: 6   KKEGEDQKKEETKQEQEEPTESAAAATEEEKKQDSKKNEEEPPSEIVLKVDMHCEACARK 65
           K+ GE   K E K ++       ++  + +KK   K        EIVLKV MHCE CA +
Sbjct: 5   KQNGESDNKSEKKNQK----NGDSSVDKSDKKNQCK--------EIVLKVYMHCEGCASQ 64

Query: 66  VARSLRGFQGVETVTTASRAGKVVVKGKEVDPLKVCERLQKKSGRKVELISPLPKPPEQQ 125
           V+  LRG+ GVE + T     KVVV GK  DPLK+  R+QKK  R  E+IS     P+  
Sbjct: 65  VSHCLRGYDGVEHIKTEIGDNKVVVSGKFDDPLKILRRVQKKFSRNAEMIS-----PKHN 124

Query: 126 PKEDEKQPKEEKKEEATPPPPAVVTVVLQVQMHCEACAQGLRKRIRKIKGVESVETEPSN 185
           PK+D+K+P ++KKE A    P + T +L++ MHCE C   +++ I KIKG++SVE + S 
Sbjct: 125 PKQDQKEP-QQKKESA----PEIKTAILRMNMHCEGCVHEIKRGIEKIKGIQSVEPDRSK 184

Query: 186 NRVIVKGVMDATTLVNEVSKRTRKPASIVVKEEEKKEEEKKEEEKPAAEEKPEEKKETQE 245
           + V+V+GVMD   LV ++ K+  K A ++ +  EK ++  K         K   KKE  E
Sbjct: 185 STVVVRGVMDPPKLVEKIKKKLGKHAELLSQITEKGKDNNK---------KNNNKKE--E 244

Query: 246 EDDEKFDIKRFEHWPTKHYTEYAYYYPERIFSDENPNGCSIM 288
            D  K        +P ++ +++A  YP +IFSDEN + CSIM
Sbjct: 245 SDGNK-----IFSYPPQYSSQHA--YPSQIFSDENVHSCSIM 246

BLAST of Carg07886 vs. ExPASy Swiss-Prot
Match: Q9FLU5 (Heavy metal-associated isoprenylated plant protein 9 OS=Arabidopsis thaliana OX=3702 GN=HIPP09 PE=2 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 1.0e-26
Identity = 115/337 (34.12%), Postives = 166/337 (49.26%), Query Frame = 0

Query: 1   MGEEIKKEGEDQKK-------EETKQEQEEPTESAAAATEEEKKQDSKKNEEEPPSEIVL 60
           MGEE+K E ++          EE +++++   E   AA EE+ K++ +     PP   +L
Sbjct: 1   MGEEVKPEAKEAASAPQAVPAEEEEKKKDVAEEKKVAAEEEKPKEEEEPQPPPPPPPFIL 60

Query: 61  KVDMHCEACARKVARSLRGFQGVETVTTASRAGKVVVKGKEVDPLKVCERLQKKSGRKVE 120
            VD+HC  CA+K+ RS+   +GVE V       +V +KG  +DP  VC +++KK+ R  +
Sbjct: 61  YVDLHCVGCAKKIERSILKIRGVEEVVMDMNENQVTIKG-VLDPQAVCNKIKKKTKRMAK 120

Query: 121 LISPLPKPPEQQPKEDEKQPKEEKKEEATPPPPAV--------VTVVLQVQMHCEACAQG 180
           ++SPLP                    E  P PP +         TV L V MHC+ACA  
Sbjct: 121 VLSPLP------------------AAEGEPLPPIITSQVSGGLTTVELSVNMHCQACADQ 180

Query: 181 LRKRIRKIKGVESVETEPSNNRVIVKGVMDATTLVNEVSKRTRKPASIV----------- 240
           L+K+I K++GV++  TE +  +VIV G MDA  LV+ V +RT+K A IV           
Sbjct: 181 LKKKILKMRGVQTTVTEHTTGKVIVTGTMDAEKLVDYVYRRTKKQARIVPQPDPEPEAPA 240

Query: 241 VKEEEKKEEEKKEEEKP--AAEEKPEEKKETQEE-------------DDEKFDIKRFEHW 287
             +EEKKEE  +  EKP    EEK EEKK+  EE             ++E+ D +     
Sbjct: 241 AAQEEKKEESGEGNEKPPETGEEKEEEKKKEGEENGEEGGGEEAAATEEERRDNEMTAMA 300

BLAST of Carg07886 vs. ExPASy Swiss-Prot
Match: Q9FJH5 (Heavy metal-associated isoprenylated plant protein 3 OS=Arabidopsis thaliana OX=3702 GN=HIPP03 PE=1 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 8.7e-26
Identity = 97/276 (35.14%), Postives = 153/276 (55.43%), Query Frame = 0

Query: 34  EEKKQDSKKNEEEPPSEIVLKVDMHCEACARKVARSLRGFQGVETVTTASRAGKVVVKGK 93
           ++K  D+KK  E P   +VLKVDMHCE CA ++ + +R FQGVETV + S  GK+ V G 
Sbjct: 11  KKKGGDNKKKNETPSITVVLKVDMHCEGCASRIVKCVRSFQGVETVKSESATGKLTVTG- 70

Query: 94  EVDPLKVCERLQKKSGRKVELISPLPKPPEQQPK-----EDEKQPKEEKK---EEATPPP 153
            +DP+K+ E+L++K+ +KV+L+SP PK  +++       ED+K+ +E+KK    +  P  
Sbjct: 71  ALDPVKLREKLEEKTKKKVDLVSPQPKKEKEKENKNKNDEDKKKSEEKKKPDNNDKKPKE 130

Query: 154 PAVVTVVLQVQMHCEACAQGLRKRIRKIKGVESVETEPSNNRVIVKGVMDATTLVNEVSK 213
             V T VL++  HC+ C   ++K + K KGV  +  +   N + VKG MD   LV  +S+
Sbjct: 131 TPVTTAVLKLNFHCQGCIGKIQKTVTKTKGVNGLTMDKEKNLLTVKGTMDVKKLVEILSE 190

Query: 214 RTRKPASIVVKEEEKKEE---EKKEEEKPAAEEKPEEKKETQEEDDEKFDIKRFEHWPTK 273
           + ++   IV  ++EK +E   E  E++K    +   ++K   +   E   +   E+   +
Sbjct: 191 KLKRAVEIVPPKKEKDKENGNENGEKKKGGGGDGGGKEKTGNKGGGE--GVNMMEYMAAQ 250

Query: 274 HYTEYAYY------YP-----ERIFSDENPNGCSIM 288
               Y YY      YP      +IFSDENPN C +M
Sbjct: 251 PAYGYGYYPGGPYGYPIQAHAPQIFSDENPNACVVM 283

BLAST of Carg07886 vs. ExPASy Swiss-Prot
Match: Q9ZUV1 (Heavy metal-associated isoprenylated plant protein 1 OS=Arabidopsis thaliana OX=3702 GN=HIPP01 PE=2 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 6.5e-21
Identity = 73/209 (34.93%), Postives = 118/209 (56.46%), Query Frame = 0

Query: 20  EQEEPTESAAAATEEEKKQDSKKNEEEPPSEIVLKVDMHCEACARKVARSLRGFQGVETV 79
           E  +  E      EEEKK+  K+N    P  +VLK+D HC+ C  ++ R  R  +GVETV
Sbjct: 2   ENPQVCECYHGDVEEEKKK--KQNNTTSPVHVVLKIDFHCDGCIARIVRLSRRLEGVETV 61

Query: 80  TTASRAGKVVVKGKEVDPLKVCERLQKKSGRKVELISPLPKPPEQQPKEDEKQPKEEKKE 139
                + K+ + G  +DP+K+ E+LQKKS +KVELISP       +PK+D K+  E+K  
Sbjct: 62  RADPDSNKLTLIGFIMDPVKIAEKLQKKSKKKVELISP-------KPKKDTKENNEKKAN 121

Query: 140 EATPPPPAVVTVVLQVQMHCEACAQGLRKRIRKIKGVESVETEPSNNRVIVKGVMDATTL 199
           + T    AV TVVL+V   C+ C + ++K +   KGV  V+ +     V V G MD  ++
Sbjct: 122 DKTQTVVAVTTVVLKVNCSCDGCIKRIQKAVSTTKGVYQVKMDKEKETVTVMGTMDIKSV 181

Query: 200 VNEVSKRTRKPASIVVKEEEKKEEEKKEE 229
            + + ++ +K   +V ++++KK+++   E
Sbjct: 182 TDNLKRKLKKTVQVVPEKKKKKKDKDNAE 201

BLAST of Carg07886 vs. ExPASy TrEMBL
Match: A0A6J1EFD3 (heavy metal-associated isoprenylated plant protein 7-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111433800 PE=3 SV=1)

HSP 1 Score: 505.8 bits (1301), Expect = 1.2e-139
Identity = 282/287 (98.26%), Postives = 284/287 (98.95%), Query Frame = 0

Query: 1   MGEEIKKEGEDQKKEETKQEQEEPTESAAAATEEEKKQDSKKNEEEPPSEIVLKVDMHCE 60
           MGEEIKKEGEDQKKEETKQEQEEPTESAAAATEEEKKQDSKKNEEE PSEIVLKVDMHCE
Sbjct: 1   MGEEIKKEGEDQKKEETKQEQEEPTESAAAATEEEKKQDSKKNEEESPSEIVLKVDMHCE 60

Query: 61  ACARKVARSLRGFQGVETVTTASRAGKVVVKGKEVDPLKVCERLQKKSGRKVELISPLPK 120
           ACARKVAR+LRGFQGVETVTT SRAGKVVVKGKEVDPLKVCERLQKKSGRKVELISPLPK
Sbjct: 61  ACARKVARALRGFQGVETVTTDSRAGKVVVKGKEVDPLKVCERLQKKSGRKVELISPLPK 120

Query: 121 PPEQQPKEDEKQPKEEKKEEATPPPPAVVTVVLQVQMHCEACAQGLRKRIRKIKGVESVE 180
           PPEQQPKEDEKQPKEEKKEEATPPPPAVVTVVLQVQMHCEACAQGLRKRIRKIKGVESVE
Sbjct: 121 PPEQQPKEDEKQPKEEKKEEATPPPPAVVTVVLQVQMHCEACAQGLRKRIRKIKGVESVE 180

Query: 181 TEPSNNRVIVKGVMDATTLVNEVSKRTRKPASIVVKEEEKKEEEKKEEEKPAAEEKPEEK 240
           TEPSNNRVIVKGVMDATTLVNEVSKRTRKPASIVVKEEEKKEEEKKE EKPAAEEKPEEK
Sbjct: 181 TEPSNNRVIVKGVMDATTLVNEVSKRTRKPASIVVKEEEKKEEEKKEVEKPAAEEKPEEK 240

Query: 241 KETQEEDDEKFDIKRFEHWPTKHYTEYAYYYPERIFSDENPNGCSIM 288
           KETQEEDD+KFDIKRFEHWPTKHYTEYAYYYPERIFSDENPNGCSIM
Sbjct: 241 KETQEEDDKKFDIKRFEHWPTKHYTEYAYYYPERIFSDENPNGCSIM 287

BLAST of Carg07886 vs. ExPASy TrEMBL
Match: A0A6J1EJ79 (heavy metal-associated isoprenylated plant protein 7-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111433800 PE=3 SV=1)

HSP 1 Score: 499.6 bits (1285), Expect = 8.8e-138
Identity = 281/287 (97.91%), Postives = 283/287 (98.61%), Query Frame = 0

Query: 1   MGEEIKKEGEDQKKEETKQEQEEPTESAAAATEEEKKQDSKKNEEEPPSEIVLKVDMHCE 60
           MGEEIKKEGEDQKKEETKQEQEEPTESAAAATEEEKKQDSKKNEEE PSEIVLKVDMHCE
Sbjct: 1   MGEEIKKEGEDQKKEETKQEQEEPTESAAAATEEEKKQDSKKNEEESPSEIVLKVDMHCE 60

Query: 61  ACARKVARSLRGFQGVETVTTASRAGKVVVKGKEVDPLKVCERLQKKSGRKVELISPLPK 120
           ACARKVAR+LRGFQGVETVTT SRAGKVVVKGKEVDPLKVCERLQKKSGRKVELISPLPK
Sbjct: 61  ACARKVARALRGFQGVETVTTDSRAGKVVVKGKEVDPLKVCERLQKKSGRKVELISPLPK 120

Query: 121 PPEQQPKEDEKQPKEEKKEEATPPPPAVVTVVLQVQMHCEACAQGLRKRIRKIKGVESVE 180
           PPEQQPKEDEKQPKEEKK EATPPPPAVVTVVLQVQMHCEACAQGLRKRIRKIKGVESVE
Sbjct: 121 PPEQQPKEDEKQPKEEKK-EATPPPPAVVTVVLQVQMHCEACAQGLRKRIRKIKGVESVE 180

Query: 181 TEPSNNRVIVKGVMDATTLVNEVSKRTRKPASIVVKEEEKKEEEKKEEEKPAAEEKPEEK 240
           TEPSNNRVIVKGVMDATTLVNEVSKRTRKPASIVVKEEEKKEEEKKE EKPAAEEKPEEK
Sbjct: 181 TEPSNNRVIVKGVMDATTLVNEVSKRTRKPASIVVKEEEKKEEEKKEVEKPAAEEKPEEK 240

Query: 241 KETQEEDDEKFDIKRFEHWPTKHYTEYAYYYPERIFSDENPNGCSIM 288
           KETQEEDD+KFDIKRFEHWPTKHYTEYAYYYPERIFSDENPNGCSIM
Sbjct: 241 KETQEEDDKKFDIKRFEHWPTKHYTEYAYYYPERIFSDENPNGCSIM 286

BLAST of Carg07886 vs. ExPASy TrEMBL
Match: A0A6J1KP38 (heavy metal-associated isoprenylated plant protein 7-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111497001 PE=3 SV=1)

HSP 1 Score: 475.7 bits (1223), Expect = 1.4e-130
Identity = 269/289 (93.08%), Postives = 278/289 (96.19%), Query Frame = 0

Query: 1   MGEEIKK--EGEDQKKEETKQEQEEPTESAAAATEEEKKQDSKKNEEEPPSEIVLKVDMH 60
           MGEEIK+  EGEDQKKEETKQ+++EPTESAAAA   EKKQD+KKNEEEPPSEIVLKVDMH
Sbjct: 1   MGEEIKRKEEGEDQKKEETKQDKQEPTESAAAA-GTEKKQDTKKNEEEPPSEIVLKVDMH 60

Query: 61  CEACARKVARSLRGFQGVETVTTASRAGKVVVKGKEVDPLKVCERLQKKSGRKVELISPL 120
           CEACARKVAR+L+GFQGVE VTT SRAGKVVVKGKE DPLKVC+RLQKKSGRKVELISPL
Sbjct: 61  CEACARKVARALKGFQGVENVTTDSRAGKVVVKGKEADPLKVCQRLQKKSGRKVELISPL 120

Query: 121 PKPPEQQPKEDEKQPKEEKKEEATPPPPAVVTVVLQVQMHCEACAQGLRKRIRKIKGVES 180
           PKPPEQQPKEDEKQPKEEKKEEATPPPP VVTVVLQVQMHCEACAQGLRKRIRKIKGVES
Sbjct: 121 PKPPEQQPKEDEKQPKEEKKEEATPPPPPVVTVVLQVQMHCEACAQGLRKRIRKIKGVES 180

Query: 181 VETEPSNNRVIVKGVMDATTLVNEVSKRTRKPASIVVKEEEKKEEEKKEEEKPAAEEKPE 240
           VETE SNNRVIVKGVMDATTLVNEVSKRTRKPASIVVKEEEKKEEEKKEEEKPA EEKPE
Sbjct: 181 VETELSNNRVIVKGVMDATTLVNEVSKRTRKPASIVVKEEEKKEEEKKEEEKPATEEKPE 240

Query: 241 EKKETQEEDDEKFDIKRFEHWPTKHYTEYAYYYPERIFSDENPNGCSIM 288
           EKKETQEEDD+KFDIKRFEHWPTKHYTEYAYYYPERIFSDENPNGCSIM
Sbjct: 241 EKKETQEEDDKKFDIKRFEHWPTKHYTEYAYYYPERIFSDENPNGCSIM 288

BLAST of Carg07886 vs. ExPASy TrEMBL
Match: A0A6J1KMC7 (heavy metal-associated isoprenylated plant protein 7-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111497001 PE=3 SV=1)

HSP 1 Score: 469.5 bits (1207), Expect = 9.7e-129
Identity = 268/289 (92.73%), Postives = 277/289 (95.85%), Query Frame = 0

Query: 1   MGEEIKK--EGEDQKKEETKQEQEEPTESAAAATEEEKKQDSKKNEEEPPSEIVLKVDMH 60
           MGEEIK+  EGEDQKKEETKQ+++EPTESAAAA   EKKQD+KKNEEEPPSEIVLKVDMH
Sbjct: 1   MGEEIKRKEEGEDQKKEETKQDKQEPTESAAAA-GTEKKQDTKKNEEEPPSEIVLKVDMH 60

Query: 61  CEACARKVARSLRGFQGVETVTTASRAGKVVVKGKEVDPLKVCERLQKKSGRKVELISPL 120
           CEACARKVAR+L+GFQGVE VTT SRAGKVVVKGKE DPLKVC+RLQKKSGRKVELISPL
Sbjct: 61  CEACARKVARALKGFQGVENVTTDSRAGKVVVKGKEADPLKVCQRLQKKSGRKVELISPL 120

Query: 121 PKPPEQQPKEDEKQPKEEKKEEATPPPPAVVTVVLQVQMHCEACAQGLRKRIRKIKGVES 180
           PKPPEQQPKEDEKQPKEEKK EATPPPP VVTVVLQVQMHCEACAQGLRKRIRKIKGVES
Sbjct: 121 PKPPEQQPKEDEKQPKEEKK-EATPPPPPVVTVVLQVQMHCEACAQGLRKRIRKIKGVES 180

Query: 181 VETEPSNNRVIVKGVMDATTLVNEVSKRTRKPASIVVKEEEKKEEEKKEEEKPAAEEKPE 240
           VETE SNNRVIVKGVMDATTLVNEVSKRTRKPASIVVKEEEKKEEEKKEEEKPA EEKPE
Sbjct: 181 VETELSNNRVIVKGVMDATTLVNEVSKRTRKPASIVVKEEEKKEEEKKEEEKPATEEKPE 240

Query: 241 EKKETQEEDDEKFDIKRFEHWPTKHYTEYAYYYPERIFSDENPNGCSIM 288
           EKKETQEEDD+KFDIKRFEHWPTKHYTEYAYYYPERIFSDENPNGCSIM
Sbjct: 241 EKKETQEEDDKKFDIKRFEHWPTKHYTEYAYYYPERIFSDENPNGCSIM 287

BLAST of Carg07886 vs. ExPASy TrEMBL
Match: A0A6J1GDA6 (heavy metal-associated isoprenylated plant protein 7-like OS=Cucurbita moschata OX=3662 GN=LOC111453145 PE=3 SV=1)

HSP 1 Score: 408.3 bits (1048), Expect = 2.7e-110
Identity = 245/293 (83.62%), Postives = 266/293 (90.78%), Query Frame = 0

Query: 1   MGEEIKK--EGEDQKKEETKQEQEEPTESAAAAT-EEEKKQDSKKNEEEPPSEIVLKVDM 60
           MGEEIKK  EGEDQKKEE K+E++E TESAAA T EEEKKQ+SKKNE+E P EIVLKVDM
Sbjct: 1   MGEEIKKKEEGEDQKKEEAKEEKKEATESAAATTPEEEKKQESKKNEDESPPEIVLKVDM 60

Query: 61  HCEACARKVARSLRGFQGVETVTTASRAGKVVVKGKEVDPLKVCERLQKKSGRKVELISP 120
           HCEACARKVAR+L+GFQGVE VTT SRAGKVVVKGKE DPLKVCERLQKKSGRKVELISP
Sbjct: 61  HCEACARKVARALKGFQGVENVTTDSRAGKVVVKGKEADPLKVCERLQKKSGRKVELISP 120

Query: 121 LPK--PPEQQPKEDEKQPKEEKKEEATPPPPAVVTVVLQVQMHCEACAQGLRKRIRKIKG 180
           LPK  P EQQPKED+KQPKEEKKEEA PPPPAVVTVVL VQMHCEACAQ L+KRI+K KG
Sbjct: 121 LPKPAPEEQQPKEDDKQPKEEKKEEA-PPPPAVVTVVLNVQMHCEACAQVLKKRIKKFKG 180

Query: 181 VESVETEPSNNRVIVKGVMDATTLVNEVSKRTRKPASIVVKEEEKKEEEKKEEEKPAAEE 240
           VESVETE ++N+VIVKGVMD + LV+ VSKR+R+PASIVVKEEEKKEEEKKEEEKPAAEE
Sbjct: 181 VESVETELASNQVIVKGVMDPSKLVDHVSKRSRRPASIVVKEEEKKEEEKKEEEKPAAEE 240

Query: 241 KPEEKKETQEEDDEKFDIKRFEHWP-TKHYTEYAYYYPERIFSDENPNGCSIM 288
           KPEEKKETQEEDD+KFDIKR E+WP TK+YTEYA Y PER+FSDENPN CSIM
Sbjct: 241 KPEEKKETQEEDDKKFDIKRLEYWPSTKYYTEYA-YVPERLFSDENPNACSIM 291

BLAST of Carg07886 vs. TAIR 10
Match: AT5G50740.3 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 180.3 bits (456), Expect = 2.3e-45
Identity = 136/298 (45.64%), Postives = 187/298 (62.75%), Query Frame = 0

Query: 15  EETKQEQEEPTESAAAATEEEKKQDSKKNEEEPPSEIVLKVDMHCEACARKVARSLRGFQ 74
           EE K+ +E+ +E   A +E++K ++ KK E   P EIVLK+ MHCE CA+K+ R L+GF+
Sbjct: 3   EEDKKMEEKKSEEPQAKSEDKKPEEEKKKE---PQEIVLKIFMHCEGCAKKIHRCLKGFE 62

Query: 75  GVETVTTASRAGKVVVKGKEVDPLKVCERLQKKSGRKVELISPLPKP----PEQQPKEDE 134
           GVE VTT  +  KVVVKG++ DPLKV +RLQ+KS R+VELISP+P+P     E + KE E
Sbjct: 63  GVEDVTTDCKTSKVVVKGEKADPLKVLQRLQRKSHRQVELISPIPEPKPVSDEPEKKEKE 122

Query: 135 KQPKEEKKEEATPPPPAVVTVVLQVQMHCEACAQGLRKRIRKIKGVESVETEPSNNRVIV 194
           K   EEKKEE       VVTVVL+V MHCEACA  ++KRI ++KGVESVE +   ++V V
Sbjct: 123 KPKPEEKKEE-------VVTVVLRVHMHCEACAMEIQKRIMRMKGVESVEPDFKASQVSV 182

Query: 195 KGVMDATTLVNEVSKRTRKPASIV-----VKEEEKKEEEKKEEEKPAAEEKPEEKKETQ- 254
           KGV     LV  + KR  K A++V      K  EK++E K ++EK   E +P+E KE + 
Sbjct: 183 KGVFTPEKLVEFIYKRIGKHAAVVKQDPPPKPPEKEKETKDKDEKKKEEGQPKEGKEAKE 242

Query: 255 --------------EEDDEKFDIKRFEH-WPTKHYTEYAYYYPERIFSDENPNGCSIM 288
                         EE ++  D+K+ E+ +    Y    + YP +IFSDENPN C+I+
Sbjct: 243 NGGGGGAKGDGAAAEEGNKVVDLKKNEYQYQPPRYPVEMFAYPPQIFSDENPNACTII 290

BLAST of Carg07886 vs. TAIR 10
Match: AT5G50740.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 179.1 bits (453), Expect = 5.0e-45
Identity = 133/292 (45.55%), Postives = 183/292 (62.67%), Query Frame = 0

Query: 21  QEEPTESAAAATEEEKKQDSKKNEEEPPSEIVLKVDMHCEACARKVARSLRGFQGVETVT 80
           +E+ +E   A +E++K ++ KK E   P EIVLK+ MHCE CA+K+ R L+GF+GVE VT
Sbjct: 2   EEKKSEEPQAKSEDKKPEEEKKKE---PQEIVLKIFMHCEGCAKKIHRCLKGFEGVEDVT 61

Query: 81  TASRAGKVVVKGKEVDPLKVCERLQKKSGRKVELISPLPKP----PEQQPKEDEKQPKEE 140
           T  +  KVVVKG++ DPLKV +RLQ+KS R+VELISP+P+P     E + KE EK   EE
Sbjct: 62  TDCKTSKVVVKGEKADPLKVLQRLQRKSHRQVELISPIPEPKPVSDEPEKKEKEKPKPEE 121

Query: 141 KKEEATPPPPAVVTVVLQVQMHCEACAQGLRKRIRKIKGVESVETEPSNNRVIVKGVMDA 200
           KKEE       VVTVVL+V MHCEACA  ++KRI ++KGVESVE +   ++V VKGV   
Sbjct: 122 KKEE-------VVTVVLRVHMHCEACAMEIQKRIMRMKGVESVEPDFKASQVSVKGVFTP 181

Query: 201 TTLVNEVSKRTRKPASIV-----VKEEEKKEEEKKEEEKPAAEEKPEEKKETQ------- 260
             LV  + KR  K A++V      K  EK++E K ++EK   E +P+E KE +       
Sbjct: 182 EKLVEFIYKRIGKHAAVVKQDPPPKPPEKEKETKDKDEKKKEEGQPKEGKEAKENGGGGG 241

Query: 261 --------EEDDEKFDIKRFEH-WPTKHYTEYAYYYPERIFSDENPNGCSIM 288
                   EE ++  D+K+ E+ +    Y    + YP +IFSDENPN C+I+
Sbjct: 242 AKGDGAAAEEGNKVVDLKKNEYQYQPPRYPVEMFAYPPQIFSDENPNACTII 283

BLAST of Carg07886 vs. TAIR 10
Match: AT5G63530.1 (farnesylated protein 3 )

HSP 1 Score: 167.5 bits (423), Expect = 1.5e-41
Identity = 143/343 (41.69%), Postives = 193/343 (56.27%), Query Frame = 0

Query: 4   EIKKEGEDQKKEETKQEQEEPTESAAAATEEEKKQDSKKNEEE------PPSEIVLKVDM 63
           E KKEGED+K +  K+ ++   +     + ++ K+DS     E      PP E+VLKV M
Sbjct: 22  EEKKEGEDKKVDAEKKGEDSDKKPQEGESNKDSKEDSAPAAPEAPAPPPPPQEVVLKVYM 81

Query: 64  HCEACARKVARSLRGFQGVETVTTASRAGKVVVKGKEVDPLKVCERLQKKSGRKVELISP 123
           HCE CARKV R L+GF+GVE V T  + GKVVVKG++ DPLKV  R+Q+K+ R+V+L+SP
Sbjct: 82  HCEGCARKVRRCLKGFEGVEDVMTDCKTGKVVVKGEKADPLKVLARVQRKTHRQVQLLSP 141

Query: 124 LPKPPEQQPK--EDEKQPKEEKKEEATPPPPAVVTVVLQVQMHCEACAQGLRKRIRKIKG 183
           +P PP    K  E++K   EEKK E    PP VVTVVL+V MHCEACA  ++KRI ++KG
Sbjct: 142 IPPPPPPPEKKAEEDKPIVEEKKVE----PPVVVTVVLKVHMHCEACATEIKKRIMRMKG 201

Query: 184 VESVETEPSNNRVIVKGVMDATTLVNEVSKRTRKPASIV---------------VKEEEK 243
           VES E++  +++V VKGV +   LV  V KRT K A+I+                 E EK
Sbjct: 202 VESAESDLKSSQVTVKGVFEPQKLVEYVYKRTGKHAAIMKIDPPPPPPPEEAAAAAEGEK 261

Query: 244 KEEEKKEEEKPAAEEK------PEEKKETQ--------------EEDDEKFDIKRFEHWP 288
           KEEEK E E    E K       EEKKE                EE+ +  ++++ E+  
Sbjct: 262 KEEEKGEGESKGEEGKDDKAKTDEEKKEGDGGKGEGEAADNGGGEEEGKVVEVRKIEN-- 321

BLAST of Carg07886 vs. TAIR 10
Match: AT5G63530.2 (farnesylated protein 3 )

HSP 1 Score: 167.5 bits (423), Expect = 1.5e-41
Identity = 143/343 (41.69%), Postives = 193/343 (56.27%), Query Frame = 0

Query: 4   EIKKEGEDQKKEETKQEQEEPTESAAAATEEEKKQDSKKNEEE------PPSEIVLKVDM 63
           E KKEGED+K +  K+ ++   +     + ++ K+DS     E      PP E+VLKV M
Sbjct: 7   EEKKEGEDKKVDAEKKGEDSDKKPQEGESNKDSKEDSAPAAPEAPAPPPPPQEVVLKVYM 66

Query: 64  HCEACARKVARSLRGFQGVETVTTASRAGKVVVKGKEVDPLKVCERLQKKSGRKVELISP 123
           HCE CARKV R L+GF+GVE V T  + GKVVVKG++ DPLKV  R+Q+K+ R+V+L+SP
Sbjct: 67  HCEGCARKVRRCLKGFEGVEDVMTDCKTGKVVVKGEKADPLKVLARVQRKTHRQVQLLSP 126

Query: 124 LPKPPEQQPK--EDEKQPKEEKKEEATPPPPAVVTVVLQVQMHCEACAQGLRKRIRKIKG 183
           +P PP    K  E++K   EEKK E    PP VVTVVL+V MHCEACA  ++KRI ++KG
Sbjct: 127 IPPPPPPPEKKAEEDKPIVEEKKVE----PPVVVTVVLKVHMHCEACATEIKKRIMRMKG 186

Query: 184 VESVETEPSNNRVIVKGVMDATTLVNEVSKRTRKPASIV---------------VKEEEK 243
           VES E++  +++V VKGV +   LV  V KRT K A+I+                 E EK
Sbjct: 187 VESAESDLKSSQVTVKGVFEPQKLVEYVYKRTGKHAAIMKIDPPPPPPPEEAAAAAEGEK 246

Query: 244 KEEEKKEEEKPAAEEK------PEEKKETQ--------------EEDDEKFDIKRFEHWP 288
           KEEEK E E    E K       EEKKE                EE+ +  ++++ E+  
Sbjct: 247 KEEEKGEGESKGEEGKDDKAKTDEEKKEGDGGKGEGEAADNGGGEEEGKVVEVRKIEN-- 306

BLAST of Carg07886 vs. TAIR 10
Match: AT5G50740.4 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 142.5 bits (358), Expect = 5.2e-34
Identity = 89/166 (53.61%), Postives = 118/166 (71.08%), Query Frame = 0

Query: 15  EETKQEQEEPTESAAAATEEEKKQDSKKNEEEPPSEIVLKVDMHCEACARKVARSLRGFQ 74
           EE K+ +E+ +E   A +E++K ++ KK E   P EIVLK+ MHCE CA+K+ R L+GF+
Sbjct: 3   EEDKKMEEKKSEEPQAKSEDKKPEEEKKKE---PQEIVLKIFMHCEGCAKKIHRCLKGFE 62

Query: 75  GVETVTTASRAGKVVVKGKEVDPLKVCERLQKKSGRKVELISPLPKP----PEQQPKEDE 134
           GVE VTT  +  KVVVKG++ DPLKV +RLQ+KS R+VELISP+P+P     E + KE E
Sbjct: 63  GVEDVTTDCKTSKVVVKGEKADPLKVLQRLQRKSHRQVELISPIPEPKPVSDEPEKKEKE 122

Query: 135 KQPKEEKKEEATPPPPAVVTVVLQVQMHCEACAQGLRKRIRKIKGV 177
           K   EEKKEE       VVTVVL+V MHCEACA  ++KRI ++KG+
Sbjct: 123 KPKPEEKKEE-------VVTVVLRVHMHCEACAMEIQKRIMRMKGL 158