Carg07864 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg07864
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	RING-type domain-containing protein
Location	Carg_Chr07: 1494541 .. 1495278 (+)
RNA-Seq Expression	Carg07864
Synteny	Carg07864

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATCTTCTCAGTTTGATTTTACAGGAAGCGGCCAGGCGAGAAAGGGAGGCTAGGACGATTTGGGGATTTTTCAGCGATCAAATGGACGGTGATGATGGAGGGAGGCGGCGGCAACGGCGGAGTTTGAAGCAACGGCTAGGGTTTAAAGTCATGCGCTGTTGTGGTGCTACTTGGGGTTTCCGGCCGGCTACGGTCAGTGTTAGAGGCGGCGATGACGATGACGACAACGACGACCGGATTGAACCGGATGTGGAGGTGATGAACACGCCACAATTTCCGGAGGAAAGGGAGTTGGATCTCCGTTGCTTGAGTCCGTTTACCATTTCGTCGCCAGCGTCGTCCGGTATGAACTTGGCCACCGCTCTGGCAGCGGAGCGTCGTTTGAGAGAGTCGCCACGTGGAACGGAGGAGGGTTTTGTGGAATCCAGCACCGAGTTCGATTCATTTGCTGGAATGATGGGAAGCGGAACGCCGTTGAGGGTGTCACTGATGCGGCTGCTGGAAGAAACAGACGGTGGCGGTGGCGATGGTGGGAATTTGGGAGTGACGGAAAAAAAGATAGAAGAGGCGAGAAATGATTCGGTGTGCTGCGTCTGCATGGGGAGGAAAAAGGGAGCAGCATTTATCCCATGTGGACACACATTTTGCAGGGTGTGTTCGAGGGAGCTGTGGTTGAATCGAGGATCATGCCCACTTTGCAACCGTCCGATCCTCGAGATTCTTGATATATTTTGA

mRNA sequence

ATGAATCTTCTCAGTTTGATTTTACAGGAAGCGGCCAGGCGAGAAAGGGAGGCTAGGACGATTTGGGGATTTTTCAGCGATCAAATGGACGGTGATGATGGAGGGAGGCGGCGGCAACGGCGGAGTTTGAAGCAACGGCTAGGGTTTAAAGTCATGCGCTGTTGTGGTGCTACTTGGGGTTTCCGGCCGGCTACGGTCAGTGTTAGAGGCGGCGATGACGATGACGACAACGACGACCGGATTGAACCGGATGTGGAGGTGATGAACACGCCACAATTTCCGGAGGAAAGGGAGTTGGATCTCCGTTGCTTGAGTCCGTTTACCATTTCGTCGCCAGCGTCGTCCGGTATGAACTTGGCCACCGCTCTGGCAGCGGAGCGTCGTTTGAGAGAGTCGCCACGTGGAACGGAGGAGGGTTTTGTGGAATCCAGCACCGAGTTCGATTCATTTGCTGGAATGATGGGAAGCGGAACGCCGTTGAGGGTGTCACTGATGCGGCTGCTGGAAGAAACAGACGGTGGCGGTGGCGATGGTGGGAATTTGGGAGTGACGGAAAAAAAGATAGAAGAGGCGAGAAATGATTCGGTGTGCTGCGTCTGCATGGGGAGGAAAAAGGGAGCAGCATTTATCCCATGTGGACACACATTTTGCAGGGTGTGTTCGAGGGAGCTGTGGTTGAATCGAGGATCATGCCCACTTTGCAACCGTCCGATCCTCGAGATTCTTGATATATTTTGA

Coding sequence (CDS)

ATGAATCTTCTCAGTTTGATTTTACAGGAAGCGGCCAGGCGAGAAAGGGAGGCTAGGACGATTTGGGGATTTTTCAGCGATCAAATGGACGGTGATGATGGAGGGAGGCGGCGGCAACGGCGGAGTTTGAAGCAACGGCTAGGGTTTAAAGTCATGCGCTGTTGTGGTGCTACTTGGGGTTTCCGGCCGGCTACGGTCAGTGTTAGAGGCGGCGATGACGATGACGACAACGACGACCGGATTGAACCGGATGTGGAGGTGATGAACACGCCACAATTTCCGGAGGAAAGGGAGTTGGATCTCCGTTGCTTGAGTCCGTTTACCATTTCGTCGCCAGCGTCGTCCGGTATGAACTTGGCCACCGCTCTGGCAGCGGAGCGTCGTTTGAGAGAGTCGCCACGTGGAACGGAGGAGGGTTTTGTGGAATCCAGCACCGAGTTCGATTCATTTGCTGGAATGATGGGAAGCGGAACGCCGTTGAGGGTGTCACTGATGCGGCTGCTGGAAGAAACAGACGGTGGCGGTGGCGATGGTGGGAATTTGGGAGTGACGGAAAAAAAGATAGAAGAGGCGAGAAATGATTCGGTGTGCTGCGTCTGCATGGGGAGGAAAAAGGGAGCAGCATTTATCCCATGTGGACACACATTTTGCAGGGTGTGTTCGAGGGAGCTGTGGTTGAATCGAGGATCATGCCCACTTTGCAACCGTCCGATCCTCGAGATTCTTGATATATTTTGA

Protein sequence

MNLLSLILQEAARREREARTIWGFFSDQMDGDDGGRRRQRRSLKQRLGFKVMRCCGATWGFRPATVSVRGGDDDDDNDDRIEPDVEVMNTPQFPEERELDLRCLSPFTISSPASSGMNLATALAAERRLRESPRGTEEGFVESSTEFDSFAGMMGSGTPLRVSLMRLLEETDGGGGDGGNLGVTEKKIEEARNDSVCCVCMGRKKGAAFIPCGHTFCRVCSRELWLNRGSCPLCNRPILEILDIF

Homology

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BLAST of Carg07864 vs. NCBI nr
Match: KAG7026653.1 (putative E3 ubiquitin-protein ligase LUL4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 502.3 bits (1292), Expect = 2.4e-138
Identity = 245/245 (100.00%), Postives = 245/245 (100.00%), Query Frame = 0


Query: 1   MNLLSLILQEAARREREARTIWGFFSDQMDGDDGGRRRQRRSLKQRLGFKVMRCCGATWG 60
           MNLLSLILQEAARREREARTIWGFFSDQMDGDDGGRRRQRRSLKQRLGFKVMRCCGATWG
Sbjct: 1   MNLLSLILQEAARREREARTIWGFFSDQMDGDDGGRRRQRRSLKQRLGFKVMRCCGATWG 60

Query: 61  FRPATVSVRGGDDDDDNDDRIEPDVEVMNTPQFPEERELDLRCLSPFTISSPASSGMNLA 120
           FRPATVSVRGGDDDDDNDDRIEPDVEVMNTPQFPEERELDLRCLSPFTISSPASSGMNLA
Sbjct: 61  FRPATVSVRGGDDDDDNDDRIEPDVEVMNTPQFPEERELDLRCLSPFTISSPASSGMNLA 120

Query: 121 TALAAERRLRESPRGTEEGFVESSTEFDSFAGMMGSGTPLRVSLMRLLEETDGGGGDGGN 180
           TALAAERRLRESPRGTEEGFVESSTEFDSFAGMMGSGTPLRVSLMRLLEETDGGGGDGGN
Sbjct: 121 TALAAERRLRESPRGTEEGFVESSTEFDSFAGMMGSGTPLRVSLMRLLEETDGGGGDGGN 180

Query: 181 LGVTEKKIEEARNDSVCCVCMGRKKGAAFIPCGHTFCRVCSRELWLNRGSCPLCNRPILE 240
           LGVTEKKIEEARNDSVCCVCMGRKKGAAFIPCGHTFCRVCSRELWLNRGSCPLCNRPILE
Sbjct: 181 LGVTEKKIEEARNDSVCCVCMGRKKGAAFIPCGHTFCRVCSRELWLNRGSCPLCNRPILE 240

Query: 241 ILDIF 246
           ILDIF
Sbjct: 241 ILDIF 245

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BLAST of Carg07864 vs. NCBI nr
Match: XP_022926821.1 (uncharacterized protein LOC111433822 isoform X1 [Cucurbita moschata])

HSP 1 Score: 495.7 bits (1275), Expect = 2.2e-136
Identity = 242/245 (98.78%), Postives = 243/245 (99.18%), Query Frame = 0


Query: 1   MNLLSLILQEAARREREARTIWGFFSDQMDGDDGGRRRQRRSLKQRLGFKVMRCCGATWG 60
           MNLLSLILQEAARREREARTIWGFFSDQMDGDDGGRRRQRRSLKQRLGFKVMRCCGATWG
Sbjct: 1   MNLLSLILQEAARREREARTIWGFFSDQMDGDDGGRRRQRRSLKQRLGFKVMRCCGATWG 60

Query: 61  FRPATVSVRGGDDDDDNDDRIEPDVEVMNTPQFPEERELDLRCLSPFTISSPASSGMNLA 120
           FRPATVSVRGGDDDDD+DDRIEPDVEVMNTPQFPE RELDLRCLSPF ISSPASSGMNLA
Sbjct: 61  FRPATVSVRGGDDDDDDDDRIEPDVEVMNTPQFPEGRELDLRCLSPFNISSPASSGMNLA 120

Query: 121 TALAAERRLRESPRGTEEGFVESSTEFDSFAGMMGSGTPLRVSLMRLLEETDGGGGDGGN 180
           TALAAERRLRESPRGTEEGFVESSTEFDSFAGMMGSGTPLRVSLMRLLEETDGGGGDGGN
Sbjct: 121 TALAAERRLRESPRGTEEGFVESSTEFDSFAGMMGSGTPLRVSLMRLLEETDGGGGDGGN 180

Query: 181 LGVTEKKIEEARNDSVCCVCMGRKKGAAFIPCGHTFCRVCSRELWLNRGSCPLCNRPILE 240
           LGVTEKKIEEARNDSVCCVCMGRKKGAAFIPCGHTFCRVCSRELWLNRGSCPLCNRPILE
Sbjct: 181 LGVTEKKIEEARNDSVCCVCMGRKKGAAFIPCGHTFCRVCSRELWLNRGSCPLCNRPILE 240

Query: 241 ILDIF 246
           ILDIF
Sbjct: 241 ILDIF 245

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BLAST of Carg07864 vs. NCBI nr
Match: KAG6594685.1 (hypothetical protein SDJN03_11238, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 494.6 bits (1272), Expect = 5.0e-136
Identity = 242/245 (98.78%), Postives = 242/245 (98.78%), Query Frame = 0


Query: 1   MNLLSLILQEAARREREARTIWGFFSDQMDGDDGGRRRQRRSLKQRLGFKVMRCCGATWG 60
           MNLLSLILQEAARREREARTIWGFFSDQMDGDDGGRRRQRRSLKQRLGFKVMRCCGATWG
Sbjct: 1   MNLLSLILQEAARREREARTIWGFFSDQMDGDDGGRRRQRRSLKQRLGFKVMRCCGATWG 60

Query: 61  FRPATVSVRGGDDDDDNDDRIEPDVEVMNTPQFPEERELDLRCLSPFTISSPASSGMNLA 120
           FRPATVSVRG DDDDDNDDRIEPDVEVMNTPQFPEERELDLRCLSPFTISSPASSGMNLA
Sbjct: 61  FRPATVSVRGADDDDDNDDRIEPDVEVMNTPQFPEERELDLRCLSPFTISSPASSGMNLA 120

Query: 121 TALAAERRLRESPRGTEEGFVESSTEFDSFAGMMGSGTPLRVSLMRLLEETDGGGGDGGN 180
           TALAAERRLRESPRGTEEGFVESSTEFDS AGMMGS TPLRVSLMRLLEETDGGGGDGGN
Sbjct: 121 TALAAERRLRESPRGTEEGFVESSTEFDSIAGMMGSETPLRVSLMRLLEETDGGGGDGGN 180

Query: 181 LGVTEKKIEEARNDSVCCVCMGRKKGAAFIPCGHTFCRVCSRELWLNRGSCPLCNRPILE 240
           LGVTEKKIEEARNDSVCCVCMGRKKGAAFIPCGHTFCRVCSRELWLNRGSCPLCNRPILE
Sbjct: 181 LGVTEKKIEEARNDSVCCVCMGRKKGAAFIPCGHTFCRVCSRELWLNRGSCPLCNRPILE 240

Query: 241 ILDIF 246
           ILDIF
Sbjct: 241 ILDIF 245

[Close]

BLAST of Carg07864 vs. NCBI nr
Match: XP_022926823.1 (uncharacterized protein LOC111433822 isoform X2 [Cucurbita moschata] >XP_022926824.1 uncharacterized protein LOC111433822 isoform X2 [Cucurbita moschata])

HSP 1 Score: 441.8 bits (1135), Expect = 3.8e-120
Identity = 214/217 (98.62%), Postives = 215/217 (99.08%), Query Frame = 0


Query: 29  MDGDDGGRRRQRRSLKQRLGFKVMRCCGATWGFRPATVSVRGGDDDDDNDDRIEPDVEVM 88
           MDGDDGGRRRQRRSLKQRLGFKVMRCCGATWGFRPATVSVRGGDDDDD+DDRIEPDVEVM
Sbjct: 1   MDGDDGGRRRQRRSLKQRLGFKVMRCCGATWGFRPATVSVRGGDDDDDDDDRIEPDVEVM 60

Query: 89  NTPQFPEERELDLRCLSPFTISSPASSGMNLATALAAERRLRESPRGTEEGFVESSTEFD 148
           NTPQFPE RELDLRCLSPF ISSPASSGMNLATALAAERRLRESPRGTEEGFVESSTEFD
Sbjct: 61  NTPQFPEGRELDLRCLSPFNISSPASSGMNLATALAAERRLRESPRGTEEGFVESSTEFD 120

Query: 149 SFAGMMGSGTPLRVSLMRLLEETDGGGGDGGNLGVTEKKIEEARNDSVCCVCMGRKKGAA 208
           SFAGMMGSGTPLRVSLMRLLEETDGGGGDGGNLGVTEKKIEEARNDSVCCVCMGRKKGAA
Sbjct: 121 SFAGMMGSGTPLRVSLMRLLEETDGGGGDGGNLGVTEKKIEEARNDSVCCVCMGRKKGAA 180

Query: 209 FIPCGHTFCRVCSRELWLNRGSCPLCNRPILEILDIF 246
           FIPCGHTFCRVCSRELWLNRGSCPLCNRPILEILDIF
Sbjct: 181 FIPCGHTFCRVCSRELWLNRGSCPLCNRPILEILDIF 217

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BLAST of Carg07864 vs. NCBI nr
Match: XP_023518592.1 (E3 ubiquitin-protein ligase CIP8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 436.8 bits (1122), Expect = 1.2e-118
Identity = 213/219 (97.26%), Postives = 215/219 (98.17%), Query Frame = 0


Query: 29  MDGDDGGRRRQRRSLKQRLGFKVMRCCGATWGFRPATVSVRGGDDDDDNDDRIEPDVEVM 88
           MDGDDGGRRRQRRS KQRLGFKVMRCCGATWGFRPATVSVRGGDDDDD+DDR+EPDVEVM
Sbjct: 1   MDGDDGGRRRQRRSFKQRLGFKVMRCCGATWGFRPATVSVRGGDDDDDDDDRMEPDVEVM 60

Query: 89  NTPQFPEERELDLRCLSPFTISSPASSGMNLATALAAERRLRESPRGTEEGFVESSTEFD 148
           NTPQFPEERELDLRCLSPF ISSPASSGMNLATALAAERRLRESPRGTEEGFVESSTEFD
Sbjct: 61  NTPQFPEERELDLRCLSPFNISSPASSGMNLATALAAERRLRESPRGTEEGFVESSTEFD 120

Query: 149 SFAGMMGSGTPLRVSLMRLLEETD--GGGGDGGNLGVTEKKIEEARNDSVCCVCMGRKKG 208
           SFAGMMGSGTPLRVSLMRLLEETD  GGGGDGGNLGVTEKKIEEARNDSVCCVCMGRKKG
Sbjct: 121 SFAGMMGSGTPLRVSLMRLLEETDGGGGGGDGGNLGVTEKKIEEARNDSVCCVCMGRKKG 180

Query: 209 AAFIPCGHTFCRVCSRELWLNRGSCPLCNRPILEILDIF 246
           AAFIPCGHTFCRVCSRELWLNRGSCPLCNRPILEILDIF
Sbjct: 181 AAFIPCGHTFCRVCSRELWLNRGSCPLCNRPILEILDIF 219

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BLAST of Carg07864 vs. ExPASy Swiss-Prot
Match: Q8LA32 (Probable E3 ubiquitin-protein ligase LUL4 OS=Arabidopsis thaliana OX=3702 GN=LUL4 PE=2 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 1.0e-06
Identity = 28/86 (32.56%), Postives = 42/86 (48.84%), Query Frame = 0


Query: 160 LRVSLMRLLEETDGGGGDGGNLGVTEKKIEEARNDSVCCVCMGRKKGAAFIPCGH-TFCR 219
           +R  L  L   T  G   G         ++E+ + + C +CM   K  A +PC H   C 
Sbjct: 274 VRYELRELYGSTTQGAASG---------LDESGSGTECVICMTEAKDTAVLPCRHLCMCS 333

Query: 220 VCSRELWLNRGSCPLCNRPILEILDI 245
            C++EL L    CP+C +PI E+L+I
Sbjct: 334 DCAKELRLQSNKCPICRQPIEELLEI 350

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BLAST of Carg07864 vs. ExPASy Swiss-Prot
Match: Q8BFW4 (Tripartite motif-containing protein 65 OS=Mus musculus OX=10090 GN=Trim65 PE=1 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 1.2e-04
Identity = 18/45 (40.00%), Postives = 27/45 (60.00%), Query Frame = 0


Query: 197 CCVCMGRKKGAAFIPCGHTFCRVCSRELWLN-RGSCPLCNRPILE 241
           C +C+GR +    +PCGH+FC  C ++ W +   SCP C +P  E
Sbjct: 13  CSICLGRYRDPVTLPCGHSFCGNCIQDSWRSCEKSCPECRQPFPE 57

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BLAST of Carg07864 vs. ExPASy Swiss-Prot
Match: Q8IUD6 (E3 ubiquitin-protein ligase RNF135 OS=Homo sapiens OX=9606 GN=RNF135 PE=1 SV=2)

HSP 1 Score: 47.8 bits (112), Expect = 2.1e-04
Identity = 20/50 (40.00%), Postives = 26/50 (52.00%), Query Frame = 0


Query: 191 ARNDSVCCVCMGRKKGAAFIPCGHTFCRVCSRELW----LNRGSCPLCNR 237
           A +D  C +C G     A +PCGH+FCR C   LW      R +CP C +
Sbjct: 15  AEDDLGCIICQGLLDWPATLPCGHSFCRHCLEALWGARDARRWACPTCRQ 64

[Close]

BLAST of Carg07864 vs. ExPASy TrEMBL
Match: A0A6J1EFY3 (uncharacterized protein LOC111433822 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111433822 PE=4 SV=1)

HSP 1 Score: 495.7 bits (1275), Expect = 1.1e-136
Identity = 242/245 (98.78%), Postives = 243/245 (99.18%), Query Frame = 0


Query: 1   MNLLSLILQEAARREREARTIWGFFSDQMDGDDGGRRRQRRSLKQRLGFKVMRCCGATWG 60
           MNLLSLILQEAARREREARTIWGFFSDQMDGDDGGRRRQRRSLKQRLGFKVMRCCGATWG
Sbjct: 1   MNLLSLILQEAARREREARTIWGFFSDQMDGDDGGRRRQRRSLKQRLGFKVMRCCGATWG 60

Query: 61  FRPATVSVRGGDDDDDNDDRIEPDVEVMNTPQFPEERELDLRCLSPFTISSPASSGMNLA 120
           FRPATVSVRGGDDDDD+DDRIEPDVEVMNTPQFPE RELDLRCLSPF ISSPASSGMNLA
Sbjct: 61  FRPATVSVRGGDDDDDDDDRIEPDVEVMNTPQFPEGRELDLRCLSPFNISSPASSGMNLA 120

Query: 121 TALAAERRLRESPRGTEEGFVESSTEFDSFAGMMGSGTPLRVSLMRLLEETDGGGGDGGN 180
           TALAAERRLRESPRGTEEGFVESSTEFDSFAGMMGSGTPLRVSLMRLLEETDGGGGDGGN
Sbjct: 121 TALAAERRLRESPRGTEEGFVESSTEFDSFAGMMGSGTPLRVSLMRLLEETDGGGGDGGN 180

Query: 181 LGVTEKKIEEARNDSVCCVCMGRKKGAAFIPCGHTFCRVCSRELWLNRGSCPLCNRPILE 240
           LGVTEKKIEEARNDSVCCVCMGRKKGAAFIPCGHTFCRVCSRELWLNRGSCPLCNRPILE
Sbjct: 181 LGVTEKKIEEARNDSVCCVCMGRKKGAAFIPCGHTFCRVCSRELWLNRGSCPLCNRPILE 240

Query: 241 ILDIF 246
           ILDIF
Sbjct: 241 ILDIF 245

[Close]

BLAST of Carg07864 vs. ExPASy TrEMBL
Match: A0A6J1EJA9 (uncharacterized protein LOC111433822 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111433822 PE=4 SV=1)

HSP 1 Score: 441.8 bits (1135), Expect = 1.9e-120
Identity = 214/217 (98.62%), Postives = 215/217 (99.08%), Query Frame = 0


Query: 29  MDGDDGGRRRQRRSLKQRLGFKVMRCCGATWGFRPATVSVRGGDDDDDNDDRIEPDVEVM 88
           MDGDDGGRRRQRRSLKQRLGFKVMRCCGATWGFRPATVSVRGGDDDDD+DDRIEPDVEVM
Sbjct: 1   MDGDDGGRRRQRRSLKQRLGFKVMRCCGATWGFRPATVSVRGGDDDDDDDDRIEPDVEVM 60

Query: 89  NTPQFPEERELDLRCLSPFTISSPASSGMNLATALAAERRLRESPRGTEEGFVESSTEFD 148
           NTPQFPE RELDLRCLSPF ISSPASSGMNLATALAAERRLRESPRGTEEGFVESSTEFD
Sbjct: 61  NTPQFPEGRELDLRCLSPFNISSPASSGMNLATALAAERRLRESPRGTEEGFVESSTEFD 120

Query: 149 SFAGMMGSGTPLRVSLMRLLEETDGGGGDGGNLGVTEKKIEEARNDSVCCVCMGRKKGAA 208
           SFAGMMGSGTPLRVSLMRLLEETDGGGGDGGNLGVTEKKIEEARNDSVCCVCMGRKKGAA
Sbjct: 121 SFAGMMGSGTPLRVSLMRLLEETDGGGGDGGNLGVTEKKIEEARNDSVCCVCMGRKKGAA 180

Query: 209 FIPCGHTFCRVCSRELWLNRGSCPLCNRPILEILDIF 246
           FIPCGHTFCRVCSRELWLNRGSCPLCNRPILEILDIF
Sbjct: 181 FIPCGHTFCRVCSRELWLNRGSCPLCNRPILEILDIF 217

[Close]

BLAST of Carg07864 vs. ExPASy TrEMBL
Match: A0A6J1KPA2 (E3 ubiquitin-protein ligase CIP8-like OS=Cucurbita maxima OX=3661 GN=LOC111497056 PE=4 SV=1)

HSP 1 Score: 432.2 bits (1110), Expect = 1.5e-117
Identity = 212/219 (96.80%), Postives = 214/219 (97.72%), Query Frame = 0


Query: 29  MDGDDGGRRRQRRSLKQRLGFKVMRCCGATWGFRPATVSVRGGDDDDDNDDRIEPDVEVM 88
           MDGDDGGRRR+RRSLKQRLGFKVMRCCGATWGFRPATVSVRGGDDDDD+DDRIEPDVEVM
Sbjct: 1   MDGDDGGRRRRRRSLKQRLGFKVMRCCGATWGFRPATVSVRGGDDDDDDDDRIEPDVEVM 60

Query: 89  NTPQFPEERELDLRCLSPFTISSPASSGMNLATALAAERRLRESPRGTEEGFVESSTEFD 148
           NTPQFPEERELDLRCLSPF ISSPASSGMNLATALAAERRLRESPRGTEEGFVESSTEF 
Sbjct: 61  NTPQFPEERELDLRCLSPFNISSPASSGMNLATALAAERRLRESPRGTEEGFVESSTEFA 120

Query: 149 SFAGMMGSGTPLRVSLMRLLEETD--GGGGDGGNLGVTEKKIEEARNDSVCCVCMGRKKG 208
           SFAGMMGSGTPLRVSLMRLLEETD  GGGG GGNLGVTEKKIEEARNDSVCCVCMGRKKG
Sbjct: 121 SFAGMMGSGTPLRVSLMRLLEETDGGGGGGGGGNLGVTEKKIEEARNDSVCCVCMGRKKG 180

Query: 209 AAFIPCGHTFCRVCSRELWLNRGSCPLCNRPILEILDIF 246
           AAFIPCGHTFCRVCSRELWLNRGSCPLCNRPILEILDIF
Sbjct: 181 AAFIPCGHTFCRVCSRELWLNRGSCPLCNRPILEILDIF 219

[Close]

BLAST of Carg07864 vs. ExPASy TrEMBL
Match: A0A6J1ISH8 (uncharacterized protein LOC111478017 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111478017 PE=4 SV=1)

HSP 1 Score: 370.5 bits (950), Expect = 5.2e-99
Identity = 188/246 (76.42%), Postives = 207/246 (84.15%), Query Frame = 0


Query: 1   MNLLSLILQEAARREREARTIWGFFSDQMDGDDGGRRRQRRSLKQRLGFKVMRCCGATWG 60
           M+LL +ILQEAARRE EARTIWGF SDQMDG DGG RR R+SLKQRLGFKVM CCGATWG
Sbjct: 1   MSLLRVILQEAARRETEARTIWGFLSDQMDGVDGGGRR-RQSLKQRLGFKVMGCCGATWG 60

Query: 61  FRPATVSVRGGDDDDDNDDRIEPDVEVMNTPQFPEERELDLRCLSPFTISSPASSGMNLA 120
            RPAT SVRGG DDDD+D R+ P+ EV N  +F EER+LD  C+SP ++ SPA+SGMNLA
Sbjct: 61  SRPATGSVRGGGDDDDDDQRV-PETEVTNPRRFQEERQLDRSCMSPLSVPSPATSGMNLA 120

Query: 121 TALAAERRLRESPRGTEEGFVESS-TEFDSFAGMMGSGTPLRVSLMRLLEETDGGGGDGG 180
           TALAAERRLR SPRG E GFVESS  +FDS  GMM + TPL+VSL+RLL+ETDG  G  G
Sbjct: 121 TALAAERRLRASPRGAEGGFVESSNNDFDSLGGMMVTETPLKVSLIRLLKETDGSDGGRG 180

Query: 181 NLGVTEKKIEEARNDSVCCVCMGRKKGAAFIPCGHTFCRVCSRELWLNRGSCPLCNRPIL 240
            LGV EKK EEA NDS+CCVCMGRKKGAAFIPCGHTFCRVCSRELWLNRG CPLCNRPI+
Sbjct: 181 ILGVAEKKREEAGNDSMCCVCMGRKKGAAFIPCGHTFCRVCSRELWLNRGFCPLCNRPIM 240

Query: 241 EILDIF 246
           EILDIF
Sbjct: 241 EILDIF 244

[Close]

BLAST of Carg07864 vs. ExPASy TrEMBL
Match: A0A6J1GH19 (uncharacterized protein LOC111453829 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111453829 PE=4 SV=1)

HSP 1 Score: 368.2 bits (944), Expect = 2.6e-98
Identity = 186/246 (75.61%), Postives = 207/246 (84.15%), Query Frame = 0


Query: 1   MNLLSLILQEAARREREARTIWGFFSDQMDGDDGGRRRQRRSLKQRLGFKVMRCCGATWG 60
           M+LL +ILQEAARRE EARTIWGF SDQMDG DGG RR R+SLKQRLGFKVM CCGATWG
Sbjct: 1   MSLLRVILQEAARRETEARTIWGFLSDQMDGVDGGGRR-RQSLKQRLGFKVMGCCGATWG 60

Query: 61  FRPATVSVRGGDDDDDNDDRIEPDVEVMNTPQFPEERELDLRCLSPFTISSPASSGMNLA 120
            RPAT SVRGG  DDD+DD+  P+ EVMN  +F +ER+LD   +SP ++ SPA+SGMNLA
Sbjct: 61  SRPATGSVRGGGGDDDDDDQRVPETEVMNPRRFQDERQLDRSFMSPLSVPSPATSGMNLA 120

Query: 121 TALAAERRLRESPRGTEEGFVESS-TEFDSFAGMMGSGTPLRVSLMRLLEETDGGGGDGG 180
           TALAAERRLR SPRG E GFVESS  +FDS  GMM + TPL+VSL+RLL+ETDG  G  G
Sbjct: 121 TALAAERRLRASPRGAEVGFVESSNNDFDSLGGMMVTETPLKVSLIRLLKETDGSDGGRG 180

Query: 181 NLGVTEKKIEEARNDSVCCVCMGRKKGAAFIPCGHTFCRVCSRELWLNRGSCPLCNRPIL 240
           +LGV EKK EEA NDS+CCVCMGRKKGAAFIPCGHTFCRVCSRELWLNRG CPLCNRPI+
Sbjct: 181 SLGVAEKKREEAGNDSMCCVCMGRKKGAAFIPCGHTFCRVCSRELWLNRGFCPLCNRPIM 240

Query: 241 EILDIF 246
           EILDIF
Sbjct: 241 EILDIF 245

[Close]

BLAST of Carg07864 vs. TAIR 10
Match: AT4G03965.1 (RING/U-box superfamily protein )

HSP 1 Score: 188.3 bits (477), Expect = 7.1e-48
Identity = 120/256 (46.88%), Postives = 144/256 (56.25%), Query Frame = 0


Query: 1   MNLLSLILQEAARREREARTIWGFFSDQMDGDDGGRRRQRRSLKQRLGFKVMRCCGATWG 60
           M+ L +   +A     ++ T       QMDG D  RRR +R+LK+RLGFK + CCG TWG
Sbjct: 1   MSQLGVETSQAQPETPQSTTFLDLLRLQMDGLDRSRRR-KRTLKERLGFKRIGCCGPTWG 60

Query: 61  FRPATVSVRGGDDDDDNDDRIEPDVEVMNTPQFPEERELDLRCLSPFTISSPASSGMNLA 120
            R    S  G DDD+  ++R+    +                      ++ P + GMNLA
Sbjct: 61  LR--LTSNTGEDDDEPFENRLVSGSD---------------------HVTGPLNQGMNLA 120

Query: 121 TALAAERRLRESPRGTEEGFVESSTEFDSFAGMMGSGTPLRVSLMRLLEETDGGGGDGGN 180
           TALAAER  R     TEE   E+S          GS TPL+VSLMRLLEET         
Sbjct: 121 TALAAERNYR-----TEE--TEAS----------GSLTPLKVSLMRLLEET------AER 180

Query: 181 LGVTEKKIEE-----------ARNDSVCCVCMGRKKGAAFIPCGHTFCRVCSRELWLNRG 240
           + V E + E              NDSVCCVCMGRKKGAAFIPCGHTFCRVCSRE+WLNRG
Sbjct: 181 VVVEENETERVTASASSTVRGVNNDSVCCVCMGRKKGAAFIPCGHTFCRVCSREVWLNRG 209

Query: 241 SCPLCNRPILEILDIF 246
           SCPLCNRPI+EILDI+
Sbjct: 241 SCPLCNRPIIEILDIY 209

[Close]

BLAST of Carg07864 vs. TAIR 10
Match: AT4G22250.1 (RING/U-box superfamily protein )

HSP 1 Score: 167.2 bits (422), Expect = 1.7e-41
Identity = 111/240 (46.25%), Postives = 123/240 (51.25%), Query Frame = 0


Query: 9   QEAARREREARTIWGFFSDQMDGDDGGRRRQRRSLKQRLGFKVMRCCGATWGFRPATVSV 68
           Q      R   T       QM G D  RR  +R+LK+RL FK + CCG TW  R    + 
Sbjct: 11  QRMTETHRTTTTFLDLLRRQMSGHDLTRR--KRTLKERLRFKCIGCCGPTWNLRLTNNTT 70

Query: 69  RGGDDDDDNDDRIEPDVEVMNTPQFPEERELDLRCLSPFTISSPASSGMNLATALAAERR 128
                    DD IE               EL +           + +GMNLATAL AER 
Sbjct: 71  L--HSPTSRDDEIE---------------ELQIETGLVLESDHGSGTGMNLATALEAERY 130

Query: 129 LRESPRGTEEGFVESSTEFDSFAGMMGSGTPLRVSLMRLLEETDGG--GGDGGNLGVTEK 188
            R             STE ++        TP RVSLMRLLEET       DG    +   
Sbjct: 131 NR-----------GESTEAEA------DMTPRRVSLMRLLEETAEKIVDDDGKETEILTA 190

Query: 189 KIEE-ARNDSVCCVCMGRKKGAAFIPCGHTFCRVCSRELWLNRGSCPLCNRPILEILDIF 246
            I     NDSVCCVCMGRKKGAAFIPCGHTFCRVCSRELWLNRGSCPLCNRPI+EILDIF
Sbjct: 191 SIGTLTGNDSVCCVCMGRKKGAAFIPCGHTFCRVCSRELWLNRGSCPLCNRPIIEILDIF 214

[Close]

BLAST of Carg07864 vs. TAIR 10
Match: AT1G62370.1 (RING/U-box superfamily protein )

HSP 1 Score: 133.7 bits (335), Expect = 2.1e-31
Identity = 95/250 (38.00%), Postives = 128/250 (51.20%), Query Frame = 0


Query: 1   MNLLSLILQEAARREREARTIWGFFSDQMDGDDGGRRRQRR--SLKQRLGFKVMRCCGAT 60
           M+ L  +L+E+    R  RT+     ++ D  +GG R   +  +LKQRL F  + CCG  
Sbjct: 1   MSQLGDLLRESEDGTRSERTMMMSLLEE-DQINGGDRTMSKWTTLKQRLRFDWVGCCG-- 60

Query: 61  WGFRPATVSVRGGDDD---DDNDDRIEPDVEVMNTPQFPEERELDLRCLSPFTISSPASS 120
              +P T+ +R  +     DD+DD  E   +V++        ELD  CL+         S
Sbjct: 61  ---KPLTLRLRQAETPIVVDDDDDEEESQNQVVDFSDPGTGTELD--CLT-------RGS 120

Query: 121 GMNLATALAAERRLRESPRGTEEGFVESSTEFDSFAGMMGSGTPLRVSLMRLLEETDGGG 180
             NLA ALA ER    +   T                        +V LMRLL E+DG  
Sbjct: 121 TRNLAEALAEERLAHVTEEET---------------------AVTKVPLMRLLAESDGCD 180

Query: 181 GDGGNLGVTEKKIEEARNDSVCCVCMGRKKGAAFIPCGHTFCRVCSRELWLNRGSCPLCN 240
                LG          ND +CCVCMGR+KGAAFIPCGHT+CRVCSRE+W+NRG+CPLCN
Sbjct: 181 STTTWLG----------NDPLCCVCMGREKGAAFIPCGHTYCRVCSREIWMNRGTCPLCN 204

Query: 241 RPILEILDIF 246
           R I ++LD++
Sbjct: 241 RSIFDVLDLY 204

[Close]

BLAST of Carg07864 vs. TAIR 10
Match: AT3G25030.1 (RING/U-box superfamily protein )

HSP 1 Score: 110.5 bits (275), Expect = 1.9e-24
Identity = 51/88 (57.95%), Postives = 62/88 (70.45%), Query Frame = 0


Query: 159 PLRVSLMRLLEETDGGGGDGGNLGVTEKKIEEARNDSV-CCVCMGRKKGAAFIPCGHTFC 218
           P R+SLM LLEE +G        G  E+++       + CCVCM R KGAAFIPCGHTFC
Sbjct: 163 PARMSLMELLEENEGQMSFVSVDGEAEEEVAAVTAAEISCCVCMVRSKGAAFIPCGHTFC 222

Query: 219 RVCSRELWLNRGSCPLCNRPILEILDIF 246
           R+CSRELW+ RG+CPLCN  ILE+LD+F
Sbjct: 223 RLCSRELWVQRGNCPLCNTTILEVLDLF 250

[Close]

BLAST of Carg07864 vs. TAIR 10
Match: AT3G25030.2 (RING/U-box superfamily protein )

HSP 1 Score: 110.5 bits (275), Expect = 1.9e-24
Identity = 51/88 (57.95%), Postives = 62/88 (70.45%), Query Frame = 0


Query: 159 PLRVSLMRLLEETDGGGGDGGNLGVTEKKIEEARNDSV-CCVCMGRKKGAAFIPCGHTFC 218
           P R+SLM LLEE +G        G  E+++       + CCVCM R KGAAFIPCGHTFC
Sbjct: 163 PARMSLMELLEENEGQMSFVSVDGEAEEEVAAVTAAEISCCVCMVRSKGAAFIPCGHTFC 222

Query: 219 RVCSRELWLNRGSCPLCNRPILEILDIF 246
           R+CSRELW+ RG+CPLCN  ILE+LD+F
Sbjct: 223 RLCSRELWVQRGNCPLCNTTILEVLDLF 250

The following BLAST results are available for this feature:

BLAST of Carg07864 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7026653.1	2.4e-138	100.00	putative E3 ubiquitin-protein ligase LUL4, partial [Cucurbita argyrosperma subsp...	[more]
XP_022926821.1	2.2e-136	98.78	uncharacterized protein LOC111433822 isoform X1 [Cucurbita moschata]	[more]
KAG6594685.1	5.0e-136	98.78	hypothetical protein SDJN03_11238, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022926823.1	3.8e-120	98.62	uncharacterized protein LOC111433822 isoform X2 [Cucurbita moschata] >XP_0229268...	[more]
XP_023518592.1	1.2e-118	97.26	E3 ubiquitin-protein ligase CIP8-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg07864 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LA32	1.0e-06	32.56	Probable E3 ubiquitin-protein ligase LUL4 OS=Arabidopsis thaliana OX=3702 GN=LUL...	[more]
Q8BFW4	1.2e-04	40.00	Tripartite motif-containing protein 65 OS=Mus musculus OX=10090 GN=Trim65 PE=1 S...	[more]
Q8IUD6	2.1e-04	40.00	E3 ubiquitin-protein ligase RNF135 OS=Homo sapiens OX=9606 GN=RNF135 PE=1 SV=2	[more]

BLAST of Carg07864 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EFY3	1.1e-136	98.78	uncharacterized protein LOC111433822 isoform X1 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1EJA9	1.9e-120	98.62	uncharacterized protein LOC111433822 isoform X2 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1KPA2	1.5e-117	96.80	E3 ubiquitin-protein ligase CIP8-like OS=Cucurbita maxima OX=3661 GN=LOC11149705...	[more]
A0A6J1ISH8	5.2e-99	76.42	uncharacterized protein LOC111478017 isoform X1 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1GH19	2.6e-98	75.61	uncharacterized protein LOC111453829 isoform X1 OS=Cucurbita moschata OX=3662 GN...	[more]

BLAST of Carg07864 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G03965.1	7.1e-48	46.88	RING/U-box superfamily protein	[more]
AT4G22250.1	1.7e-41	46.25	RING/U-box superfamily protein	[more]
AT1G62370.1	2.1e-31	38.00	RING/U-box superfamily protein	[more]
AT3G25030.1	1.9e-24	57.95	RING/U-box superfamily protein	[more]
AT3G25030.2	1.9e-24	57.95	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 197..234 e-value: 2.8E-6 score: 36.9
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 197..235 score: 10.895954
None	No IPR available	PFAM	PF13920	zf-C3HC4_3	coord: 194..239 e-value: 9.8E-12 score: 44.5
None	No IPR available	PANTHER	PTHR46629:SF22	RING/U-BOX SUPERFAMILY PROTEIN	coord: 17..245
None	No IPR available	PANTHER	PTHR46629	OS01G0917900 PROTEIN	coord: 17..245
None	No IPR available	CDD	cd16449	RING-HC	coord: 197..234 e-value: 1.58829E-8 score: 47.4621
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 191..242
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 180..245 e-value: 3.5E-14 score: 53.9

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg07864-RA	Carg07864-RA	mRNA