Carg07731 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg07731
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	Carg_Chr13: 8671262 .. 8672408 (+)
RNA-Seq Expression	Carg07731
Synteny	Carg07731

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGGTGAGCTTCCTGTTGTGAGATTTAGTTCACACAATATGCAGAGCGGAGGGAGGAAGTGGGGTTCGACGAGGGAGAAGGTCCGGGAGGCACTTGAAGAATATGGGTGCTTTGTAGCGTTGTGCGACAGCGTTTCAATGGAGGCGAGTTCTTTGAAGATGATGGAAGGCATGAAGGAGGTGTTCGAAGTTCCATTGGAGAGGAAGGTTCAGAATGTGTCTGAGAAGCCTTTCCATGGCTACTTTGGGCAGAACCCATTGATGCCTATCCATGAAAGCATGGGCATTGAAGACCCTCTTCTTCCCTCCAACATCCAATCTTTCTCCAACCTCATGTGGCCCTCTCGCCCAAACCCTCTCTTCTGGTAAATTTCTTCCCTTCATTCATTCTGCCTTTATTTCTCACTTTCAGAGCTAACCCTCATGCTATTTTGCAGTGAAAACCTCAAATCCTACGCACAATTGGTGTCCCAATTGGAGCAGACGGTGAAGAAGATGGTGTTCGAGAGCTACGGCCTCAAAAGCCTCTTCCTTTCTCACACCCAATCCACCAAATATCTTCTCCGGATGATCAAATACAGAGTCCCACATCGGAACGAGATGAATTTGGGAGCTTTCCCTCACACCGACAAGAGCTTCCTCACCATTCTTCATCAGAATTCAGTAAATGGGTTACACATTAAGACCAGAGACAACAGATGGGTTGAATATCAGCCTCCTTCTACAGAATCTTCTGCCAAATCATTCATCGTCATGGCTGGAGATGCCTTCTTTGTAAGTTCCCATTAACTTCAAATTCCATTACAATTAGCAATAACCCATCAAAAACTTACCAACAATTGTGGGGTTGTTGGGACAGGCGTGGAGCAATGGGAGGATATATTCGGCGCCGCACAGAGTGACGATGAGTGGAAACAGAGAGAGATACTCAGTGGGGTTGTTTTCGTTCAACGATGGGATTGTTCAGATTCCAAAAGAATTGGTGGACGACAAACATCCGCAGCAGTTTGAGGCGTTTGATCATCAAGATTACCTTCGTTTCTACAGCACTGAGAAAGGCCAAAGCTCCCCTTCTGCTATTAAAGCTTATTGCGGCGTTGCAAATGGCCTCCTTGATTCACGAACTTGCATTCCCATGCATTAA

mRNA sequence

ATGGCGGGTGAGCTTCCTGTTGTGAGATTTAGTTCACACAATATGCAGAGCGGAGGGAGGAAGTGGGGTTCGACGAGGGAGAAGGTCCGGGAGGCACTTGAAGAATATGGGTGCTTTGTAGCGTTGTGCGACAGCGTTTCAATGGAGGCGAGTTCTTTGAAGATGATGGAAGGCATGAAGGAGGTGTTCGAAGTTCCATTGGAGAGGAAGGTTCAGAATGTGTCTGAGAAGCCTTTCCATGGCTACTTTGGGCAGAACCCATTGATGCCTATCCATGAAAGCATGGGCATTGAAGACCCTCTTCTTCCCTCCAACATCCAATCTTTCTCCAACCTCATGTGGCCCTCTCGCCCAAACCCTCTCTTCTGTGAAAACCTCAAATCCTACGCACAATTGGTGTCCCAATTGGAGCAGACGGTGAAGAAGATGGTGTTCGAGAGCTACGGCCTCAAAAGCCTCTTCCTTTCTCACACCCAATCCACCAAATATCTTCTCCGGATGATCAAATACAGAGTCCCACATCGGAACGAGATGAATTTGGGAGCTTTCCCTCACACCGACAAGAGCTTCCTCACCATTCTTCATCAGAATTCAGTAAATGGGTTACACATTAAGACCAGAGACAACAGATGGGTTGAATATCAGCCTCCTTCTACAGAATCTTCTGCCAAATCATTCATCGTCATGGCTGGAGATGCCTTCTTTGCGTGGAGCAATGGGAGGATATATTCGGCGCCGCACAGAGTGACGATGAGTGGAAACAGAGAGAGATACTCAGTGGGGTTGTTTTCGTTCAACGATGGGATTGTTCAGATTCCAAAAGAATTGGTGGACGACAAACATCCGCAGCAGTTTGAGGCGTTTGATCATCAAGATTACCTTCGTTTCTACAGCACTGAGAAAGGCCAAAGCTCCCCTTCTGCTATTAAAGCTTATTGCGGCGTTGCAAATGGCCTCCTTGATTCACGAACTTGCATTCCCATGCATTAA

Coding sequence (CDS)

ATGGCGGGTGAGCTTCCTGTTGTGAGATTTAGTTCACACAATATGCAGAGCGGAGGGAGGAAGTGGGGTTCGACGAGGGAGAAGGTCCGGGAGGCACTTGAAGAATATGGGTGCTTTGTAGCGTTGTGCGACAGCGTTTCAATGGAGGCGAGTTCTTTGAAGATGATGGAAGGCATGAAGGAGGTGTTCGAAGTTCCATTGGAGAGGAAGGTTCAGAATGTGTCTGAGAAGCCTTTCCATGGCTACTTTGGGCAGAACCCATTGATGCCTATCCATGAAAGCATGGGCATTGAAGACCCTCTTCTTCCCTCCAACATCCAATCTTTCTCCAACCTCATGTGGCCCTCTCGCCCAAACCCTCTCTTCTGTGAAAACCTCAAATCCTACGCACAATTGGTGTCCCAATTGGAGCAGACGGTGAAGAAGATGGTGTTCGAGAGCTACGGCCTCAAAAGCCTCTTCCTTTCTCACACCCAATCCACCAAATATCTTCTCCGGATGATCAAATACAGAGTCCCACATCGGAACGAGATGAATTTGGGAGCTTTCCCTCACACCGACAAGAGCTTCCTCACCATTCTTCATCAGAATTCAGTAAATGGGTTACACATTAAGACCAGAGACAACAGATGGGTTGAATATCAGCCTCCTTCTACAGAATCTTCTGCCAAATCATTCATCGTCATGGCTGGAGATGCCTTCTTTGCGTGGAGCAATGGGAGGATATATTCGGCGCCGCACAGAGTGACGATGAGTGGAAACAGAGAGAGATACTCAGTGGGGTTGTTTTCGTTCAACGATGGGATTGTTCAGATTCCAAAAGAATTGGTGGACGACAAACATCCGCAGCAGTTTGAGGCGTTTGATCATCAAGATTACCTTCGTTTCTACAGCACTGAGAAAGGCCAAAGCTCCCCTTCTGCTATTAAAGCTTATTGCGGCGTTGCAAATGGCCTCCTTGATTCACGAACTTGCATTCCCATGCATTAA

Protein sequence

MAGELPVVRFSSHNMQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEGMKEVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLFCENLKSYAQLVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLGAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSTESSAKSFIVMAGDAFFAWSNGRIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFEAFDHQDYLRFYSTEKGQSSPSAIKAYCGVANGLLDSRTCIPMH

Homology

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BLAST of Carg07731 vs. NCBI nr
Match: KAG7019869.1 (putative 2-oxoglutarate-dependent dioxygenase AOP1.2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 678.3 bits (1749), Expect = 3.3e-191
Identity = 329/329 (100.00%), Postives = 329/329 (100.00%), Query Frame = 0


Query: 1   MAGELPVVRFSSHNMQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEGMK 60
           MAGELPVVRFSSHNMQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEGMK
Sbjct: 1   MAGELPVVRFSSHNMQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEGMK 60

Query: 61  EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120
           EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP
Sbjct: 61  EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120

Query: 121 LFCENLKSYAQLVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180
           LFCENLKSYAQLVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL
Sbjct: 121 LFCENLKSYAQLVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180

Query: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSTESSAKSFIVMAGDAFFAWSNG 240
           GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSTESSAKSFIVMAGDAFFAWSNG
Sbjct: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSTESSAKSFIVMAGDAFFAWSNG 240

Query: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFEAFDHQDYLRFYSTE 300
           RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFEAFDHQDYLRFYSTE
Sbjct: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFEAFDHQDYLRFYSTE 300

Query: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 330
           KGQSSPSAIKAYCGVANGLLDSRTCIPMH
Sbjct: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 329

[Close]

BLAST of Carg07731 vs. NCBI nr
Match: KAG6584272.1 (putative 2-oxoglutarate-dependent dioxygenase AOP1.2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 673.3 bits (1736), Expect = 1.0e-189
Identity = 326/329 (99.09%), Postives = 328/329 (99.70%), Query Frame = 0


Query: 1   MAGELPVVRFSSHNMQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEGMK 60
           MAGELPVVRFSSHN+QSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMME MK
Sbjct: 1   MAGELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAMK 60

Query: 61  EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120
           EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP
Sbjct: 61  EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120

Query: 121 LFCENLKSYAQLVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180
           LFCENLKSYAQLVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL
Sbjct: 121 LFCENLKSYAQLVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180

Query: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSTESSAKSFIVMAGDAFFAWSNG 240
           GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSTESSAKSFIVMAGDAFFAWSNG
Sbjct: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSTESSAKSFIVMAGDAFFAWSNG 240

Query: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFEAFDHQDYLRFYSTE 300
           RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQF+AFDHQDYLRFYSTE
Sbjct: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE 300

Query: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 330
           KGQSSPSAIKAYCGVANGLLDSRTCIPMH
Sbjct: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 329

[Close]

BLAST of Carg07731 vs. NCBI nr
Match: KAG6584273.1 (putative 2-oxoglutarate-dependent dioxygenase AOP1.2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 669.1 bits (1725), Expect = 2.0e-188
Identity = 325/329 (98.78%), Postives = 327/329 (99.39%), Query Frame = 0


Query: 1   MAGELPVVRFSSHNMQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEGMK 60
           MAGELPVVRFSSHN+QSGGRKWGSTREKVREALEEYGCFVAL DSVSMEASSLKMME MK
Sbjct: 1   MAGELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALYDSVSMEASSLKMMEAMK 60

Query: 61  EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120
           EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP
Sbjct: 61  EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120

Query: 121 LFCENLKSYAQLVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180
           LFCENLKSYAQLVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL
Sbjct: 121 LFCENLKSYAQLVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180

Query: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSTESSAKSFIVMAGDAFFAWSNG 240
           GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSTESSAKSFIVMAGDAFFAWSNG
Sbjct: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSTESSAKSFIVMAGDAFFAWSNG 240

Query: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFEAFDHQDYLRFYSTE 300
           RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQF+AFDHQDYLRFYSTE
Sbjct: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE 300

Query: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 330
           KGQSSPSAIKAYCGVANGLLDSRTCIPMH
Sbjct: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 329

[Close]

BLAST of Carg07731 vs. NCBI nr
Match: XP_022923887.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita moschata])

HSP 1 Score: 668.3 bits (1723), Expect = 3.4e-188
Identity = 322/329 (97.87%), Postives = 327/329 (99.39%), Query Frame = 0


Query: 1   MAGELPVVRFSSHNMQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEGMK 60
           MAGELPVVRFSSHN+QSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMME M+
Sbjct: 1   MAGELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAME 60

Query: 61  EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120
           EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP
Sbjct: 61  EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120

Query: 121 LFCENLKSYAQLVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180
           LFCENLKSYA+LVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL
Sbjct: 121 LFCENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180

Query: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSTESSAKSFIVMAGDAFFAWSNG 240
           GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPS ESSAKSFI+MAGDAFFAWSNG
Sbjct: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIIMAGDAFFAWSNG 240

Query: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFEAFDHQDYLRFYSTE 300
           RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQF+AFDHQDYLRFYSTE
Sbjct: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE 300

Query: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 330
           KGQSSPSAIKAYCGVANGLLDSRTCIPMH
Sbjct: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 329

[Close]

BLAST of Carg07731 vs. NCBI nr
Match: XP_022923888.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita moschata] >XP_023519885.1 probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita pepo subsp. pepo] >XP_023519886.1 probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 665.2 bits (1715), Expect = 2.9e-187
Identity = 322/329 (97.87%), Postives = 326/329 (99.09%), Query Frame = 0


Query: 1   MAGELPVVRFSSHNMQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEGMK 60
           MAGE+PVVRFSSHN+QSGGRKWGSTREKVREALEEYGCFVAL DSVSMEASSLKMME MK
Sbjct: 1   MAGEIPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALYDSVSMEASSLKMMEAMK 60

Query: 61  EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120
           EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP
Sbjct: 61  EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120

Query: 121 LFCENLKSYAQLVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180
           LFCENLKSYA+LVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL
Sbjct: 121 LFCENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180

Query: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSTESSAKSFIVMAGDAFFAWSNG 240
           GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPS ESSAKSFIVMAGDAFFAWSNG
Sbjct: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIVMAGDAFFAWSNG 240

Query: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFEAFDHQDYLRFYSTE 300
           RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQF+AFDHQDYLRFYSTE
Sbjct: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE 300

Query: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 330
           KGQSSPSAIKAYCGVANGLLDSRTCIPMH
Sbjct: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 329

[Close]

BLAST of Carg07731 vs. ExPASy Swiss-Prot
Match: Q945B6 (Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=3702 GN=AOP1.2 PE=2 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 1.4e-64
Identity = 124/316 (39.24%), Postives = 194/316 (61.39%), Query Frame = 0


Query: 4   ELPVVRFSSHNMQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEGMKEVF 63
           +LPV+ FS  N++ G  KW   +  V +ALE+YGCF A  D +S+E +   + E M+++F
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVKADVLKALEDYGCFEAFFDKLSVELNR-SVFEAMEDLF 72

Query: 64  EVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLFC 123
           E+P+  K +NVS KPFHGY   N    ++ES+GI+D  +   +  F+  +WP   N    
Sbjct: 73  ELPIPTKQRNVSSKPFHGYLCHN----LYESLGIDDANVLEKVNDFTQQLWPDHGNKSIS 132

Query: 124 ENLKSYAQLVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVP-----HRNEM 183
           E +  +++ + +L+  V++M+ ES+G+++    H  ST YL R++KY  P        E 
Sbjct: 133 ETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDEET 192

Query: 184 NLGAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSTESSAKSFIVMAGDAFFAWS 243
            LG   HTDK+ +TILHQ  V+GL +KT+D++W++ +P     S  S +VM GD+  A  
Sbjct: 193 KLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKP-----SQDSVLVMVGDSLCALL 252

Query: 244 NGRIYSAPHRVTMSGNRERYSVGLFSF--NDGIVQIPKELVDDKHPQQFEAFDHQDYLRF 303
           NGR++S  HRV M+G + RYS GLFS      I+  P+ELVD +HP+ F+ F++ D+L F
Sbjct: 253 NGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHF 312

Query: 304 YSTEKGQSSPSAIKAY 313
           + TE G+ + SA+ A+
Sbjct: 313 FQTEAGRIAQSALHAF 318

[Close]

BLAST of Carg07731 vs. ExPASy Swiss-Prot
Match: Q9ZTA3 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 245.0 bits (624), Expect = 1.2e-63
Identity = 124/317 (39.12%), Postives = 192/317 (60.57%), Query Frame = 0


Query: 4   ELPVVRFSSHNMQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEGMKEVF 63
           +LPV+ FS  N++ G  KW      V +ALE+YGCF A  D +S+E +   + E M+++F
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNR-SVFEAMEDLF 72

Query: 64  EVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLFC 123
           E+P+  K +NVS KPFHGY   N    ++ES+GI D  +   +  F+  +WP   N    
Sbjct: 73  ELPIPTKQRNVSSKPFHGYLCHN----LYESLGINDANVLEKVNDFTQQLWPDHGNKSIS 132

Query: 124 ENLKSYAQLVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVP------HRNE 183
           E +  +++ + +L+  V++M+ ES+G+++    H  ST YL R++KY  P         E
Sbjct: 133 ETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDDEE 192

Query: 184 MNLGAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSTESSAKSFIVMAGDAFFAW 243
             LG   HTDK+ +TILHQ  V+GL +KT+D++W++ +P     S  S +VM GD+  A 
Sbjct: 193 TKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKP-----SQDSVLVMVGDSLCAL 252

Query: 244 SNGRIYSAPHRVTMSGNRERYSVGLFSF--NDGIVQIPKELVDDKHPQQFEAFDHQDYLR 303
            NGR++S  HRV M+G + RYS GLFS      I+  P+ELVD +HP+ F+ F++ D+L 
Sbjct: 253 LNGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLH 312

Query: 304 FYSTEKGQSSPSAIKAY 313
           F+ TE G+ + SA+ A+
Sbjct: 313 FFQTEAGRIAQSALHAF 319

[Close]

BLAST of Carg07731 vs. ExPASy Swiss-Prot
Match: Q944Z5 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 7.3e-61
Identity = 120/306 (39.22%), Postives = 184/306 (60.13%), Query Frame = 0


Query: 4   ELPVVRFSSHNMQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEGMKEVF 63
           +LPV+ FS  N++ G  KW      V +ALE+YGCF A  D +S+E +   + E M+++F
Sbjct: 6   QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNR-SVFEAMEDLF 65

Query: 64  EVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLFC 123
           E+P+  K +NVS K FHGY   N    ++ES+GI D  +   +  F+  +WP   N    
Sbjct: 66  ELPIPTKQRNVSSKLFHGYLCHN----LYESLGINDANVLEKVNDFTQQLWPDHGNKSIS 125

Query: 124 ENLKSYAQLVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVP----HRNEMN 183
           E +  +++ + +L+  V++M+ ES+G++     H  ST YL R++KY  P       E  
Sbjct: 126 ETIHLFSEQLVELDLMVRRMIMESFGIEKYIDEHLNSTYYLTRLMKYTSPPDDDDDEETK 185

Query: 184 LGAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSTESSAKSFIVMAGDAFFAWSN 243
           LG   HTDK+ +TILHQ  V+GL +KT+D++W++ +P     S  S +VM GD+  A  N
Sbjct: 186 LGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKP-----SQDSVLVMVGDSLCALLN 245

Query: 244 GRIYSAPHRVTMSGNRERYSVGLFSF--NDGIVQIPKELVDDKHPQQFEAFDHQDYLRFY 303
           GR++S  HRV M+G + RYS GLFS      I+  P+ELVD +HP+ F+ F++ D+L F+
Sbjct: 246 GRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFF 301

[Close]

BLAST of Carg07731 vs. ExPASy Swiss-Prot
Match: A0A0N9HQ36 (Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=1 SV=1)

HSP 1 Score: 218.4 bits (555), Expect = 1.2e-55
Identity = 117/308 (37.99%), Postives = 179/308 (58.12%), Query Frame = 0


Query: 5   LPVVRFSSHNMQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEGMKEVFE 64
           LPV+  S  N++ G   W S R +VREALEEYGCF A+ D+VS E       +G  E+  
Sbjct: 9   LPVIDLSMKNLKPGTTSWNSVRTQVREALEEYGCFEAVIDAVSPELQKAVCNKG-HELLN 68

Query: 65  VPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLFCE 124
           +PLE K+ N ++  + G+     +  ++E MG+        ++ F+NLMWP   N  FCE
Sbjct: 69  LPLETKMLNGNKPEYDGF---TSIPNLNEGMGVGRITDLEKVERFTNLMWP-EGNKDFCE 128

Query: 125 NLKSYAQLVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLGAFP 184
            + SY + +++++  +K MVFES+G++  F S  +ST YLL  ++Y+ P ++  +     
Sbjct: 129 TVYSYGKRMAEVDHILKMMVFESFGMEKHFDSFCESTNYLLHFMRYQQPGKDGRSPALSL 188

Query: 185 HTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSTESSAKSFIVMAGDAFFAWSNGRIYS 244
           H DKS LTI++QN V GL  +T+D  W+         +A + IV+ GD F AWSNGR++S
Sbjct: 189 HKDKSILTIVNQNDVKGLEFETKDGEWI-------LPTADNHIVLLGDCFMAWSNGRLHS 248

Query: 245 APHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFEAFDHQDYLRFYSTEKGQS 304
             HRVT+  N+ R S   FSF   I++ P ELVD++HP  F  F+  + L +  T++G  
Sbjct: 249 PLHRVTLVANQARLSTSSFSFPKDIIETPAELVDEEHPLLFNPFEITELLAYCFTKEGAK 304

Query: 305 SPSAIKAY 313
           +   +K Y
Sbjct: 309 AVCDLKQY 304

[Close]

BLAST of Carg07731 vs. ExPASy Swiss-Prot
Match: Q945B4 (2-oxoglutarate-dependent dioxygenase AOP3 OS=Arabidopsis thaliana OX=3702 GN=AOP3 PE=2 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 1.4e-48
Identity = 124/414 (29.95%), Postives = 193/414 (46.62%), Query Frame = 0


Query: 4   ELPVVRFSSHNMQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEGMKEVF 63
           +LP++  S   ++ G  KW   R  VR+ALE+YGCF A  D VSME     +++ M+E+F
Sbjct: 7   QLPLICLSDQTLKPGSSKWVKVRSDVRKALEDYGCFEAKIDQVSMELQG-SVLKAMQELF 66

Query: 64  EVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLFC 123
            +P E K +NV  KPF GYF  N L    ES GI+D  +      F+  +WP     +  
Sbjct: 67  ALPTEAKQRNVCPKPFAGYFSHNGL---SESFGIKDANILEKAHEFTQQLWPEGNKSI-- 126

Query: 124 ENLKSYAQLVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEM----- 183
           + ++ YA+ +++L+  V++++ ESYG++     H  ST Y +R++KY     N++     
Sbjct: 127 KMIQLYAEKLAELDMMVRRLILESYGIEYFIDEHLNSTYYRMRLMKYIARPDNDITAAVG 186

Query: 184 ------------------------------------------------------------ 243
                                                                       
Sbjct: 187 ANVDNGANDNADGDANVNDDGASIGVKVNVDVGDDVNDNDSVNIGVGVDINVETNVNGDL 246

Query: 244 -------------------------------NLGAFPHTDKSFLTILHQNSVNGLHIKTR 303
                                           LG   HTDKS   I++Q+ ++GL +KT+
Sbjct: 247 DAEANGDATAWVVGAVSGNASVGAKEANVDAELGLPSHTDKSLSGIIYQHQIDGLEVKTK 306

Query: 304 DNRWVEYQPPSTESSAKSFIVMAGDAFFAWSNGRIYSAPHRVTMSGNRE-RYSVGLFSF- 319
           + +W+  +P     +  + IV+AGDA  A  NGRI S  HRV ++  ++ RY+  LFS+ 
Sbjct: 307 EGKWIRVKP-----APNTVIVIAGDALCALMNGRIPSPYHRVRVTERKKTRYAAALFSYP 366

[Close]

BLAST of Carg07731 vs. ExPASy TrEMBL
Match: A0A6J1E7C8 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662 GN=LOC111431472 PE=3 SV=1)

HSP 1 Score: 668.3 bits (1723), Expect = 1.6e-188
Identity = 322/329 (97.87%), Postives = 327/329 (99.39%), Query Frame = 0


Query: 1   MAGELPVVRFSSHNMQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEGMK 60
           MAGELPVVRFSSHN+QSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMME M+
Sbjct: 1   MAGELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAME 60

Query: 61  EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120
           EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP
Sbjct: 61  EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120

Query: 121 LFCENLKSYAQLVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180
           LFCENLKSYA+LVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL
Sbjct: 121 LFCENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180

Query: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSTESSAKSFIVMAGDAFFAWSNG 240
           GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPS ESSAKSFI+MAGDAFFAWSNG
Sbjct: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIIMAGDAFFAWSNG 240

Query: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFEAFDHQDYLRFYSTE 300
           RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQF+AFDHQDYLRFYSTE
Sbjct: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE 300

Query: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 330
           KGQSSPSAIKAYCGVANGLLDSRTCIPMH
Sbjct: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 329

[Close]

BLAST of Carg07731 vs. ExPASy TrEMBL
Match: A0A6J1EAT7 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662 GN=LOC111431473 PE=3 SV=1)

HSP 1 Score: 665.2 bits (1715), Expect = 1.4e-187
Identity = 322/329 (97.87%), Postives = 326/329 (99.09%), Query Frame = 0


Query: 1   MAGELPVVRFSSHNMQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEGMK 60
           MAGE+PVVRFSSHN+QSGGRKWGSTREKVREALEEYGCFVAL DSVSMEASSLKMME MK
Sbjct: 1   MAGEIPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALYDSVSMEASSLKMMEAMK 60

Query: 61  EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120
           EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP
Sbjct: 61  EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120

Query: 121 LFCENLKSYAQLVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180
           LFCENLKSYA+LVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL
Sbjct: 121 LFCENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180

Query: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSTESSAKSFIVMAGDAFFAWSNG 240
           GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPS ESSAKSFIVMAGDAFFAWSNG
Sbjct: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIVMAGDAFFAWSNG 240

Query: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFEAFDHQDYLRFYSTE 300
           RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQF+AFDHQDYLRFYSTE
Sbjct: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE 300

Query: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 330
           KGQSSPSAIKAYCGVANGLLDSRTCIPMH
Sbjct: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 329

[Close]

BLAST of Carg07731 vs. ExPASy TrEMBL
Match: A0A6J1KFF7 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita maxima OX=3661 GN=LOC111495326 PE=3 SV=1)

HSP 1 Score: 661.8 bits (1706), Expect = 1.5e-186
Identity = 318/329 (96.66%), Postives = 326/329 (99.09%), Query Frame = 0


Query: 1   MAGELPVVRFSSHNMQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEGMK 60
           MAGE+PVVRFSSHN+QSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASS+KMME MK
Sbjct: 1   MAGEIPVVRFSSHNVQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSVKMMEAMK 60

Query: 61  EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120
           EVFEVPLERKVQNVS+KPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP
Sbjct: 61  EVFEVPLERKVQNVSQKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120

Query: 121 LFCENLKSYAQLVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180
           LFC NLKSYA+LVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL
Sbjct: 121 LFCGNLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180

Query: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSTESSAKSFIVMAGDAFFAWSNG 240
           GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPS +SSA+SFIVMAGDAFFAWSNG
Sbjct: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAQSSARSFIVMAGDAFFAWSNG 240

Query: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFEAFDHQDYLRFYSTE 300
           RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQF+AFDHQDYLRFYSTE
Sbjct: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE 300

Query: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 330
           KGQSSPSAIKAYCGVANGLLDSRTCIPMH
Sbjct: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 329

[Close]

BLAST of Carg07731 vs. ExPASy TrEMBL
Match: A0A6J1C712 (probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111008991 PE=3 SV=1)

HSP 1 Score: 507.3 bits (1305), Expect = 4.8e-140
Identity = 241/326 (73.93%), Postives = 279/326 (85.58%), Query Frame = 0


Query: 4   ELPVVRFSSHNMQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEGMKEVF 63
           +LP+V+FS  N+    R W STREKVREALEEYGCFVAL DSVSME SS  M + +KE+F
Sbjct: 5   QLPLVKFSEENL----RSWVSTREKVREALEEYGCFVALYDSVSMELSS-NMFDSLKELF 64

Query: 64  EVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLFC 123
           EVPLERK++NVSEKP+HGYFGQNPLMPIHESMGIEDP L S  QSF+NLMWPS  N  FC
Sbjct: 65  EVPLERKLKNVSEKPYHGYFGQNPLMPIHESMGIEDPNLLSKTQSFTNLMWPSNGNKSFC 124

Query: 124 ENLKSYAQLVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLGAF 183
           EN+ SYA+ +++L++TVKKMVFESYG++    SH +ST+YLLRMIKYRVP +NEMN+GAF
Sbjct: 125 ENVNSYARAIAELDETVKKMVFESYGVRKHLESHMKSTRYLLRMIKYRVPKQNEMNMGAF 184

Query: 184 PHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSTESSAKSFIVMAGDAFFAWSNGRIY 243
           PHTDKSFLTILHQN VNGL IKT+ N+W++Y P S+ SS  SFIVMAGDAFFAWSNGRIY
Sbjct: 185 PHTDKSFLTILHQNQVNGLQIKTKHNKWLQYHPSSSSSSTSSFIVMAGDAFFAWSNGRIY 244

Query: 244 SAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFEAFDHQDYLRFYSTEKGQ 303
           S PH+V MSG RERYSVGLFSFNDGIVQIPKELVD+ HPQQF+ FDHQDYLRFYSTE+GQ
Sbjct: 245 SPPHKVIMSGKRERYSVGLFSFNDGIVQIPKELVDENHPQQFKPFDHQDYLRFYSTERGQ 304

Query: 304 SSPSAIKAYCGVANGLLDSRTCIPMH 330
           +SP AIKAYCG+ NGL+D+R CI +H
Sbjct: 305 NSPCAIKAYCGIGNGLVDTRGCILLH 325

[Close]

BLAST of Carg07731 vs. ExPASy TrEMBL
Match: A0A5D3BIZ7 (Putative 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002910 PE=3 SV=1)

HSP 1 Score: 491.5 bits (1264), Expect = 2.7e-135
Identity = 239/329 (72.64%), Postives = 279/329 (84.80%), Query Frame = 0


Query: 1   MAGELPVVRFSSHNMQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEGMK 60
           M+ ELPVV+F S N++SG ++W  T +KVREALE+YGCFVAL DSVS E SS  M + +K
Sbjct: 5   MSDELPVVKFCSENLKSGTKEWVFTMKKVREALEDYGCFVALYDSVSAEVSS-NMFDSLK 64

Query: 61  EVF-EVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPN 120
           E+F +VPLERK+QNVSEKP+HGYFGQNPLMPIHESMGIE P+LP+NI SF+NLMWPSR N
Sbjct: 65  ELFDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGN 124

Query: 121 PLFCENLKSYAQLVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMN 180
             F E++ +YA+LVS+L++ VK+MVFESYG+ +   SH +STKYL+RMIKYRVP   EMN
Sbjct: 125 DSFRESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVPKEKEMN 184

Query: 181 LGAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSTESSAKSFIVMAGDAFFAWSN 240
           LGAFPHTDKSFLTILHQN VNGL IKTRDN+W++Y P S+ SS  SFIVMAGDAFFAWSN
Sbjct: 185 LGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSS-SSTSSFIVMAGDAFFAWSN 244

Query: 241 GRIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFEAFDHQDYLRFYST 300
           GRIYS PHRV MSGNRERYSVGLFSFN+GIVQIP+ELVDDKHPQ F  FDHQD+LRFYST
Sbjct: 245 GRIYSPPHRVIMSGNRERYSVGLFSFNNGIVQIPEELVDDKHPQLFNPFDHQDFLRFYST 304

Query: 301 EKGQSSPSAIKAYCGVANGLLDSRTCIPM 329
           E+GQ+SP AIKAYCGV N L+D  T  P+
Sbjct: 305 ERGQNSPCAIKAYCGVGNSLIDMDTRSPL 331

[Close]

BLAST of Carg07731 vs. TAIR 10
Match: AT1G52820.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 280.8 bits (717), Expect = 1.4e-75
Identity = 135/313 (43.13%), Postives = 208/313 (66.45%), Query Frame = 0


Query: 5   LPVVRFSSHNMQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEGMKEVFE 64
           LPV+ FS+ N++ G  +W  TR  V++AL++YG F A  D +  E     +   ++E+F+
Sbjct: 12  LPVIDFSNKNLKPGEPEWDLTRADVQKALQDYGYFEASFDRIPFELRK-SVFGALEELFD 71

Query: 65  VPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLFCE 124
           +PL+ K++NVS+KPFHGY GQ P++P++ESMGI+D  +   + +F+  +WP + N  F  
Sbjct: 72  LPLQTKLRNVSKKPFHGYVGQYPMVPLYESMGIDDSDIAEKVDAFTEKLWP-QGNISFST 131

Query: 125 NLKSYAQLVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLGAFP 184
            ++S+++ +S+L+ T+++M+ ES+GL      H  ST YLLR++KY+ P   E  +G   
Sbjct: 132 TIQSFSKKLSELDITIRRMIMESFGLDKYIDEHLHSTNYLLRVMKYKGPDTEETKVGLNA 191

Query: 185 HTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSTESSAKSFIVMAGDAFFAWSNGRIYS 244
           HTDK+ +TIL+QN V GL ++T+D  W++ +P     +  SF VM GD+ +A  NGR++S
Sbjct: 192 HTDKNIVTILYQNHVEGLEVQTKDKNWIKVKP-----TQDSFTVMIGDSLYALLNGRLHS 251

Query: 245 APHRVTMSGNRERYSVGLFSFNDG--IVQIPKELVDDKHPQQFEAFDHQDYLRFYSTEKG 304
             HRV M+G   RYS+GLFS      IV  P ELVD++HP+ F+ FDH ++L+FY TE G
Sbjct: 252 PYHRVMMTGTETRYSLGLFSIPKAGHIVSSPDELVDEEHPRLFKPFDHVEFLQFYYTEAG 311

Query: 305 QSSPSAIKAYCGV 316
           Q S SA+K YCG+
Sbjct: 312 QRSQSALKTYCGI 317

[Close]

BLAST of Carg07731 vs. TAIR 10
Match: AT1G52790.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 278.1 bits (710), Expect = 9.2e-75
Identity = 135/315 (42.86%), Postives = 206/315 (65.40%), Query Frame = 0


Query: 1   MAGELPVVRFSSHNMQSGGRKWGSTREKVREALEEYGCFVA-LCDSVSMEASSLKMMEGM 60
           M+ ++P + FS  +++ G + W STRE +R+ALEEYGCF+  L D   ++    ++   +
Sbjct: 1   MSPKIPTLDFSREDLKPGTKYWESTRENIRQALEEYGCFIIDLKDKTPLDLLD-RVFGSL 60

Query: 61  KEVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPN 120
            ++F++P + K++N  +KP +GY GQ P +P+HES+GI++       +SF+ LMWP + N
Sbjct: 61  VDLFDLPTQTKMKNKYDKPLNGYVGQIPALPLHESLGIDNATSLEATRSFTGLMWP-QGN 120

Query: 121 PLFCENLKSYAQLVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMN 180
             F E L  YA+  ++L+Q V +MVF+SY ++  +  + +ST YLLR++K R P+     
Sbjct: 121 EHFSECLYKYAEFAAELDQMVTRMVFQSYNVEKYYDPYIESTTYLLRVLKNRAPNNENPT 180

Query: 181 LGAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSTESSAKSFIVMAGDAFFAWSN 240
           LG   HTDKSF TILHQ+ VNGL ++TR+   +        SS   F+V+AGDA  AWSN
Sbjct: 181 LGFVTHTDKSFTTILHQDQVNGLEMETREGERINI----NLSSPSLFMVVAGDALMAWSN 240

Query: 241 GRIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFEAFDHQDYLRFYST 300
            R++S  H+V +SG  +RYS+G+F+FN+G +Q+P+EL+D +HP  ++ FDH   LRFY T
Sbjct: 241 DRVWSPRHQVLVSGETDRYSLGMFAFNNGTLQVPEELIDHQHPLMYKPFDHLGLLRFYRT 300

Query: 301 EKGQSSPSAIKAYCG 315
           + G  S   IKAYCG
Sbjct: 301 DTGYKSECPIKAYCG 309

[Close]

BLAST of Carg07731 vs. TAIR 10
Match: AT1G52800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 277.7 bits (709), Expect = 1.2e-74
Identity = 133/306 (43.46%), Postives = 202/306 (66.01%), Query Frame = 0


Query: 11  SSHNMQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEGMKEVFEVPLERK 70
           S   ++     W S   +  EALEEYGCF+A+ D V+ +     +    +E+F++P E K
Sbjct: 17  SQQELKPNTSTWRSVSREACEALEEYGCFLAVYDGVTQQLDD-SIFAAAEELFDLPTETK 76

Query: 71  VQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLFCENLKSYA 130
            +NV+EKP+HGY GQ P++P+HE +G++        Q F++LMWP + N  FC  + +++
Sbjct: 77  KKNVNEKPYHGYVGQMPVIPLHEGLGVDYVTNKEIAQRFTHLMWP-QGNDRFCNTVHTFS 136

Query: 131 QLVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLGAFP-HTDKS 190
             V++L++ V +M+FE+YG++  + SH  S  YLL+ +KY  P  + +++ AFP HTDK+
Sbjct: 137 NAVAELDRLVVRMIFENYGVEKHYESHVGSKTYLLKFLKYLAPPES-ISMPAFPQHTDKT 196

Query: 191 FLTILHQNSVNGLHIKTRDNRWVEYQPPSTESSAKSFIVMAGDAFFAWSNGRIYSAPHRV 250
           FL+ILHQN VNGL +K++D  W+  Q P      KS++VMAGD    WSN RI S  HRV
Sbjct: 197 FLSILHQNDVNGLEVKSKDGEWISLQLP-----PKSYVVMAGDISMGWSNDRIRSCEHRV 256

Query: 251 TMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFEAFDHQDYLRFYSTEKGQSSPSAI 310
           TM G++ RY++GLFSF   +V IP+ELVDDKHP  ++ FD+   + FY+T++G+ + S +
Sbjct: 257 TMEGDKTRYTLGLFSFLTDLVSIPEELVDDKHPLMYKPFDNIALINFYTTKEGREANSTL 314

Query: 311 KAYCGV 316
           KAYCG+
Sbjct: 317 KAYCGI 314

[Close]

BLAST of Carg07731 vs. TAIR 10
Match: AT1G80320.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 245.4 bits (625), Expect = 6.6e-65
Identity = 131/317 (41.32%), Postives = 190/317 (59.94%), Query Frame = 0


Query: 5   LPVVRFSSHNMQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEGMKEVFE 64
           LP++ FSS  +  G   W +TR+ VR A+E  G FVA    VS +     ++ GMKE++ 
Sbjct: 9   LPILDFSSDKLVRGTSHWITTRDDVRRAMEGQGWFVAEFSGVSSDLRD-NLLAGMKEMYY 68

Query: 65  VPLERKVQNVSEKPFHGYFGQ-NPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLFC 124
           +P + K++N + K  HGY         IHES+GI+        + FS L+WP   +P FC
Sbjct: 69  LPDQIKIKNENHKASHGYMSMVVDDYRIHESLGIDYATELQACKDFSKLLWPQGNDP-FC 128

Query: 125 ENLKSYAQLVSQLEQTVKKMVFESYGL--KSLFLSHTQSTKYLLRMIKYRVPHRNEMNLG 184
           +    YA  +++L+QTV +M++ESYG+  K   +SH++ST+YLLRM+ YR     E N G
Sbjct: 129 QTTHMYAMTMAELDQTVMRMLYESYGMDEKKHSVSHSESTRYLLRMLSYRRQQNGEANTG 188

Query: 185 AFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSTESSAKSFIVMAGDAFFAWSNGR 244
              HTDKSF++ILHQN V GL +KT   +WV + P  T      F+V++G    AWSN R
Sbjct: 189 FVSHTDKSFMSILHQNHVGGLQLKTMTGQWVGFNPSPTR-----FVVLSGMGLTAWSNDR 248

Query: 245 IYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFEAFDHQDYLRFYST-- 304
           I +  H+V MS +  RYS+G FSF+ G ++ P+ELVDD+HP ++  F+H   LRFY +  
Sbjct: 249 IKACYHKVVMSADEIRYSLGFFSFHKGTIRTPEELVDDQHPLRYNPFEHDGLLRFYESYI 308

Query: 305 -EKGQSSPSAIKAYCGV 316
               +SS   ++ YCGV
Sbjct: 309 NSLKKSSEDLLQIYCGV 318

[Close]

BLAST of Carg07731 vs. TAIR 10
Match: AT4G03070.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 245.0 bits (624), Expect = 8.6e-65
Identity = 124/317 (39.12%), Postives = 192/317 (60.57%), Query Frame = 0


Query: 4   ELPVVRFSSHNMQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEGMKEVF 63
           +LPV+ FS  N++ G  KW      V +ALE+YGCF A  D +S+E +   + E M+++F
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNR-SVFEAMEDLF 72

Query: 64  EVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLFC 123
           E+P+  K +NVS KPFHGY   N    ++ES+GI D  +   +  F+  +WP   N    
Sbjct: 73  ELPIPTKQRNVSSKPFHGYLCHN----LYESLGINDANVLEKVNDFTQQLWPDHGNKSIS 132

Query: 124 ENLKSYAQLVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVP------HRNE 183
           E +  +++ + +L+  V++M+ ES+G+++    H  ST YL R++KY  P         E
Sbjct: 133 ETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDDEE 192

Query: 184 MNLGAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSTESSAKSFIVMAGDAFFAW 243
             LG   HTDK+ +TILHQ  V+GL +KT+D++W++ +P     S  S +VM GD+  A 
Sbjct: 193 TKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKP-----SQDSVLVMVGDSLCAL 252

Query: 244 SNGRIYSAPHRVTMSGNRERYSVGLFSF--NDGIVQIPKELVDDKHPQQFEAFDHQDYLR 303
            NGR++S  HRV M+G + RYS GLFS      I+  P+ELVD +HP+ F+ F++ D+L 
Sbjct: 253 LNGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLH 312

Query: 304 FYSTEKGQSSPSAIKAY 313
           F+ TE G+ + SA+ A+
Sbjct: 313 FFQTEAGRIAQSALHAF 319

The following BLAST results are available for this feature:

BLAST of Carg07731 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7019869.1	3.3e-191	100.00	putative 2-oxoglutarate-dependent dioxygenase AOP1.2, partial [Cucurbita argyros...	[more]
KAG6584272.1	1.0e-189	99.09	putative 2-oxoglutarate-dependent dioxygenase AOP1.2, partial [Cucurbita argyros...	[more]
KAG6584273.1	2.0e-188	98.78	putative 2-oxoglutarate-dependent dioxygenase AOP1.2, partial [Cucurbita argyros...	[more]
XP_022923887.1	3.4e-188	97.87	probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita moschata]	[more]
XP_022923888.1	2.9e-187	97.87	probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita moschata] >XP_0235...	[more]

BLAST of Carg07731 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q945B6	1.4e-64	39.24	Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=...	[more]
Q9ZTA3	1.2e-63	39.12	Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=37...	[more]
Q944Z5	7.3e-61	39.22	Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis tha...	[more]
A0A0N9HQ36	1.2e-55	37.99	Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=...	[more]
Q945B4	1.4e-48	29.95	2-oxoglutarate-dependent dioxygenase AOP3 OS=Arabidopsis thaliana OX=3702 GN=AOP...	[more]

BLAST of Carg07731 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E7C8	1.6e-188	97.87	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662...	[more]
A0A6J1EAT7	1.4e-187	97.87	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662...	[more]
A0A6J1KFF7	1.5e-186	96.66	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita maxima OX=3661 G...	[more]
A0A6J1C712	4.8e-140	73.93	probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Momordica chara...	[more]
A0A5D3BIZ7	2.7e-135	72.64	Putative 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucumis melo var. makuwa O...	[more]

BLAST of Carg07731 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G52820.1	1.4e-75	43.13	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G52790.1	9.2e-75	42.86	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G52800.1	1.2e-74	43.46	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G80320.1	6.6e-65	41.32	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G03070.1	8.6e-65	39.12	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 3..317 e-value: 8.1E-52 score: 178.4
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 170..265 e-value: 5.2E-18 score: 65.4
None	No IPR available	PANTHER	PTHR47990:SF17	2-OXOGLUTARATE-DEPENDENT DIOXYGENASE AOP3-LIKE	coord: 4..314
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 4..314
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 4..302
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 161..268 score: 10.735442

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg07731-RA	Carg07731-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding