Carg07730 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg07730
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionPlant protein of unknown function (DUF247)
LocationCarg_Chr13: 8664289 .. 8666388 (-)
RNA-Seq ExpressionCarg07730
SyntenyCarg07730
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAACAACGCAATGAAATCATTGGATGAAGGCAACCCCTGTTCTTCCTCACCTTCAATTCTCCGATCAAAATCCGGCGAAAGAAGATGGGTCGTTTACATCAACGACATCATCCATAAACACCTTAAAAACCCCAAATCCGACATCTCTTCCTCGATCTTCATCGTCCCAAAATTCCTCAGCGCCGCAAAGCCACAACATTATACCCCGCAATTCTTGGCTTTAGGTCCTTACCACCATTTCTCTCAAGAACTTTATGATAATATGGAGCGTTATAAACTTATGATTGCCAATCAAATCAGACACCAAATTGAATTTCAATCCTTTGTGTGTCGGCTGTCCCAGCTTGACCTTCATATTCGTGGCTCATACCACCGCCGTTTGGATCTTGATGCTGATACTCTGGCTCTCATTATGGCCATCGATGCTCTGTTCTTGCTCGAGCTTCTTTATTCCCAAGTTCAAAACCATGATCTTCTCCGTCCTCTTAACAAATTCATTACTGTTTCGAGGTGCAAGAGATTGAGTAATGATGCTGTTGTGCGGGACATTGTGAAGCTCGAGAATCAGATTCCGTTGTTCGTTTTGAGAGAGATTTTCGCGGCGGTGATTCAATGCGACGCGACTGAATCCGATGATTTGCTTGCCTCTGTTTTGGTGGGTTTTTGCGTTGAAATTTCCCCTTTGAATCTGACGGTGGGTCGTCGTGGTGTCATTGATTGTGCGCATGTTTTGGATGTTTTGTACCGCTTGATTTTACCGGAGAATTTGGAATGCTCCGGCGAAGACGACGGCCCAAAGGAGCAGCCTGGGAATATCCAACAGTCGAGATCTTTGAAGGGTAATTATCTGTGGTCGTATCCTTGGAATTGGATGTTGGATTGCTTCAAAATATTGAGGGGTTCTAAGAAAGTTTTTGAATTTATTGGACGTTTGTTGGACCTGCTGCCGATTGTCGGCGGCTTGGTTTCATCCATGGAAGAAAATCCCGACGGAAACAGAGTCGAAATCTTGATAGAAGACAAGACGCCCTTAATGGAAGCCGGCATCAAGATTCCGACAGCCTCCCAACTTTTGGACACCGGTAAGTCGTGGATTGTTAGGACAAAATTTCACGTTAGCTAATTCAGTGAATGATCAAAAACACAAAAGCAAAACCATGAGAGGTAAATGAACAATATCATATCATACACAGAGTTGTAATTCTTAAAATGACATTAGAGCCATGCTCGAATTCTTAAATATGGAACAAAGAAGTTGGGATCCTCGAAAGTGTAGTCAAAAGTGACTCAATTGTCGAATAAAGGGTGTACTTTGTTTGATGACCTTAATGCTGTTCGAGATCTCACTCAAGTACCAAATAAATGATGTATTTTGTTCAAGGGTCAATTAAGGTGAAGCGGTTCGAGGGCTTTATAGACCTCAAAAGAGGCTCTATAGTGTACTTTGTTCGAGAGTAGGATTGTTGGGAAATTCAAGAATATCATACTATTGCGGAGATTCGTGATTACTAACGAAAATCTGACATGGGTGTTGATGAATTTTACAGGGGTCAGCTTCAAACAAAGCGCAAGCATTAAAGCAATAAAATTCGAGGCAGAAAGCGTGACGTTGTTCCTCCCAGTGATCAAACTGGGAGCAAATTCAGAAGTAATCCTACGAAATTTGGTTGCATACGAAGCCATGGCGATGCCAGATATCTTGGCATTCAGTCGCTACTTACATCTAATGAACAGCTTGATCGACACCGCCGAGGATGTCAAGATACTCAAAGACGCAGAGATCGTGGTGATGAATGGGATGAAGAAAGATGAGGAGGTTGCAGTCCTTTTCAATGGCATAATGACTAGCAGTTCCATGGGATTGAGCGCCGCAAAAGAGCTGGACGAGGCCATCAATGGCGTGAACAAGTACTATAAAGGGAGGCCGAAGGTGAAGGCGAGTAGGGTGATTAAGAAATATGTTTACAGTTCGTGGAGGATTCTGGCGTTGATGGCTACGCTCATGGTTTTGGGCTTGTTGGTTCTGCAATCCTTTTGCTCTGTTTATAATTGCCCTCGTTTGTTTGGTACTCTGGATATTGGGGGAGATGATTCTTGA

mRNA sequence

ATGAACAACGCAATGAAATCATTGGATGAAGGCAACCCCTGTTCTTCCTCACCTTCAATTCTCCGATCAAAATCCGGCGAAAGAAGATGGGTCGTTTACATCAACGACATCATCCATAAACACCTTAAAAACCCCAAATCCGACATCTCTTCCTCGATCTTCATCGTCCCAAAATTCCTCAGCGCCGCAAAGCCACAACATTATACCCCGCAATTCTTGGCTTTAGGTCCTTACCACCATTTCTCTCAAGAACTTTATGATAATATGGAGCGTTATAAACTTATGATTGCCAATCAAATCAGACACCAAATTGAATTTCAATCCTTTGTGTGTCGGCTGTCCCAGCTTGACCTTCATATTCGTGGCTCATACCACCGCCGTTTGGATCTTGATGCTGATACTCTGGCTCTCATTATGGCCATCGATGCTCTGTTCTTGCTCGAGCTTCTTTATTCCCAAGTTCAAAACCATGATCTTCTCCGTCCTCTTAACAAATTCATTACTGTTTCGAGGTGCAAGAGATTGAGTAATGATGCTGTTGTGCGGGACATTGTGAAGCTCGAGAATCAGATTCCGTTGTTCGTTTTGAGAGAGATTTTCGCGGCGGTGATTCAATGCGACGCGACTGAATCCGATGATTTGCTTGCCTCTGTTTTGGTGGGTTTTTGCGTTGAAATTTCCCCTTTGAATCTGACGGTGGGTCGTCGTGGTGTCATTGATTGTGCGCATGTTTTGGATGTTTTGTACCGCTTGATTTTACCGGAGAATTTGGAATGCTCCGGCGAAGACGACGGCCCAAAGGAGCAGCCTGGGAATATCCAACAGTCGAGATCTTTGAAGGGTAATTATCTGTGGTCGTATCCTTGGAATTGGATGTTGGATTGCTTCAAAATATTGAGGGAAAGCGTGACGTTGTTCCTCCCAGTGATCAAACTGGGAGCAAATTCAGAAGTAATCCTACGAAATTTGGTTGCATACGAAGCCATGGCGATGCCAGATATCTTGGCATTCAGTCGCTACTTACATCTAATGAACAGCTTGATCGACACCGCCGAGGATGTCAAGATACTCAAAGACGCAGAGATCGTGGTGATGAATGGGATGAAGAAAGATGAGGAGGTTGCAGTCCTTTTCAATGGCATAATGACTAGCAGTTCCATGGGATTGAGCGCCGCAAAAGAGCTGGACGAGGCCATCAATGGCGTGAACAAGTACTATAAAGGGAGGCCGAAGGTGAAGGCGAGTAGGGTGATTAAGAAATATGTTTACAGTTCGTGGAGGATTCTGGCGTTGATGGCTACGCTCATGGTTTTGGGCTTGTTGGTTCTGCAATCCTTTTGCTCTGTTTATAATTGCCCTCGTTTGTTTGGTACTCTGGATATTGGGGGAGATGATTCTTGA

Coding sequence (CDS)

ATGAACAACGCAATGAAATCATTGGATGAAGGCAACCCCTGTTCTTCCTCACCTTCAATTCTCCGATCAAAATCCGGCGAAAGAAGATGGGTCGTTTACATCAACGACATCATCCATAAACACCTTAAAAACCCCAAATCCGACATCTCTTCCTCGATCTTCATCGTCCCAAAATTCCTCAGCGCCGCAAAGCCACAACATTATACCCCGCAATTCTTGGCTTTAGGTCCTTACCACCATTTCTCTCAAGAACTTTATGATAATATGGAGCGTTATAAACTTATGATTGCCAATCAAATCAGACACCAAATTGAATTTCAATCCTTTGTGTGTCGGCTGTCCCAGCTTGACCTTCATATTCGTGGCTCATACCACCGCCGTTTGGATCTTGATGCTGATACTCTGGCTCTCATTATGGCCATCGATGCTCTGTTCTTGCTCGAGCTTCTTTATTCCCAAGTTCAAAACCATGATCTTCTCCGTCCTCTTAACAAATTCATTACTGTTTCGAGGTGCAAGAGATTGAGTAATGATGCTGTTGTGCGGGACATTGTGAAGCTCGAGAATCAGATTCCGTTGTTCGTTTTGAGAGAGATTTTCGCGGCGGTGATTCAATGCGACGCGACTGAATCCGATGATTTGCTTGCCTCTGTTTTGGTGGGTTTTTGCGTTGAAATTTCCCCTTTGAATCTGACGGTGGGTCGTCGTGGTGTCATTGATTGTGCGCATGTTTTGGATGTTTTGTACCGCTTGATTTTACCGGAGAATTTGGAATGCTCCGGCGAAGACGACGGCCCAAAGGAGCAGCCTGGGAATATCCAACAGTCGAGATCTTTGAAGGGTAATTATCTGTGGTCGTATCCTTGGAATTGGATGTTGGATTGCTTCAAAATATTGAGGGAAAGCGTGACGTTGTTCCTCCCAGTGATCAAACTGGGAGCAAATTCAGAAGTAATCCTACGAAATTTGGTTGCATACGAAGCCATGGCGATGCCAGATATCTTGGCATTCAGTCGCTACTTACATCTAATGAACAGCTTGATCGACACCGCCGAGGATGTCAAGATACTCAAAGACGCAGAGATCGTGGTGATGAATGGGATGAAGAAAGATGAGGAGGTTGCAGTCCTTTTCAATGGCATAATGACTAGCAGTTCCATGGGATTGAGCGCCGCAAAAGAGCTGGACGAGGCCATCAATGGCGTGAACAAGTACTATAAAGGGAGGCCGAAGGTGAAGGCGAGTAGGGTGATTAAGAAATATGTTTACAGTTCGTGGAGGATTCTGGCGTTGATGGCTACGCTCATGGTTTTGGGCTTGTTGGTTCTGCAATCCTTTTGCTCTGTTTATAATTGCCCTCGTTTGTTTGGTACTCTGGATATTGGGGGAGATGATTCTTGA

Protein sequence

MNNAMKSLDEGNPCSSSPSILRSKSGERRWVVYINDIIHKHLKNPKSDISSSIFIVPKFLSAAKPQHYTPQFLALGPYHHFSQELYDNMERYKLMIANQIRHQIEFQSFVCRLSQLDLHIRGSYHRRLDLDADTLALIMAIDALFLLELLYSQVQNHDLLRPLNKFITVSRCKRLSNDAVVRDIVKLENQIPLFVLREIFAAVIQCDATESDDLLASVLVGFCVEISPLNLTVGRRGVIDCAHVLDVLYRLILPENLECSGEDDGPKEQPGNIQQSRSLKGNYLWSYPWNWMLDCFKILRESVTLFLPVIKLGANSEVILRNLVAYEAMAMPDILAFSRYLHLMNSLIDTAEDVKILKDAEIVVMNGMKKDEEVAVLFNGIMTSSSMGLSAAKELDEAINGVNKYYKGRPKVKASRVIKKYVYSSWRILALMATLMVLGLLVLQSFCSVYNCPRLFGTLDIGGDDS
Homology
BLAST of Carg07730 vs. NCBI nr
Match: KAG7019868.1 (putative UPF0481 protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 917.5 bits (2370), Expect = 4.5e-263
Identity = 466/466 (100.00%), Postives = 466/466 (100.00%), Query Frame = 0

Query: 1   MNNAMKSLDEGNPCSSSPSILRSKSGERRWVVYINDIIHKHLKNPKSDISSSIFIVPKFL 60
           MNNAMKSLDEGNPCSSSPSILRSKSGERRWVVYINDIIHKHLKNPKSDISSSIFIVPKFL
Sbjct: 1   MNNAMKSLDEGNPCSSSPSILRSKSGERRWVVYINDIIHKHLKNPKSDISSSIFIVPKFL 60

Query: 61  SAAKPQHYTPQFLALGPYHHFSQELYDNMERYKLMIANQIRHQIEFQSFVCRLSQLDLHI 120
           SAAKPQHYTPQFLALGPYHHFSQELYDNMERYKLMIANQIRHQIEFQSFVCRLSQLDLHI
Sbjct: 61  SAAKPQHYTPQFLALGPYHHFSQELYDNMERYKLMIANQIRHQIEFQSFVCRLSQLDLHI 120

Query: 121 RGSYHRRLDLDADTLALIMAIDALFLLELLYSQVQNHDLLRPLNKFITVSRCKRLSNDAV 180
           RGSYHRRLDLDADTLALIMAIDALFLLELLYSQVQNHDLLRPLNKFITVSRCKRLSNDAV
Sbjct: 121 RGSYHRRLDLDADTLALIMAIDALFLLELLYSQVQNHDLLRPLNKFITVSRCKRLSNDAV 180

Query: 181 VRDIVKLENQIPLFVLREIFAAVIQCDATESDDLLASVLVGFCVEISPLNLTVGRRGVID 240
           VRDIVKLENQIPLFVLREIFAAVIQCDATESDDLLASVLVGFCVEISPLNLTVGRRGVID
Sbjct: 181 VRDIVKLENQIPLFVLREIFAAVIQCDATESDDLLASVLVGFCVEISPLNLTVGRRGVID 240

Query: 241 CAHVLDVLYRLILPENLECSGEDDGPKEQPGNIQQSRSLKGNYLWSYPWNWMLDCFKILR 300
           CAHVLDVLYRLILPENLECSGEDDGPKEQPGNIQQSRSLKGNYLWSYPWNWMLDCFKILR
Sbjct: 241 CAHVLDVLYRLILPENLECSGEDDGPKEQPGNIQQSRSLKGNYLWSYPWNWMLDCFKILR 300

Query: 301 ESVTLFLPVIKLGANSEVILRNLVAYEAMAMPDILAFSRYLHLMNSLIDTAEDVKILKDA 360
           ESVTLFLPVIKLGANSEVILRNLVAYEAMAMPDILAFSRYLHLMNSLIDTAEDVKILKDA
Sbjct: 301 ESVTLFLPVIKLGANSEVILRNLVAYEAMAMPDILAFSRYLHLMNSLIDTAEDVKILKDA 360

Query: 361 EIVVMNGMKKDEEVAVLFNGIMTSSSMGLSAAKELDEAINGVNKYYKGRPKVKASRVIKK 420
           EIVVMNGMKKDEEVAVLFNGIMTSSSMGLSAAKELDEAINGVNKYYKGRPKVKASRVIKK
Sbjct: 361 EIVVMNGMKKDEEVAVLFNGIMTSSSMGLSAAKELDEAINGVNKYYKGRPKVKASRVIKK 420

Query: 421 YVYSSWRILALMATLMVLGLLVLQSFCSVYNCPRLFGTLDIGGDDS 467
           YVYSSWRILALMATLMVLGLLVLQSFCSVYNCPRLFGTLDIGGDDS
Sbjct: 421 YVYSSWRILALMATLMVLGLLVLQSFCSVYNCPRLFGTLDIGGDDS 466

BLAST of Carg07730 vs. NCBI nr
Match: XP_022923882.1 (putative UPF0481 protein At3g02645 [Cucurbita moschata])

HSP 1 Score: 877.1 bits (2265), Expect = 6.8e-251
Identity = 462/544 (84.93%), Postives = 464/544 (85.29%), Query Frame = 0

Query: 1   MNNAMKSLDEGNPCSSSPSILRSKSGERRWVVYINDIIHKHLKNPKSDISSSIFIVPKFL 60
           MNNAMKSLDEGNPCSSSPSILRSKSGERRWVVYINDIIHKHLKNPKSDISSSIFIVPKFL
Sbjct: 7   MNNAMKSLDEGNPCSSSPSILRSKSGERRWVVYINDIIHKHLKNPKSDISSSIFIVPKFL 66

Query: 61  SAAKPQHYTPQFLALGPYHHFSQELYDNMERYKLMIANQIRHQIEFQSFVCRLSQLDLHI 120
           SAAKPQHYTPQFLALGPYHHFSQELYDNMERYKLMIANQIRHQIEFQSFVCRLSQLDLHI
Sbjct: 67  SAAKPQHYTPQFLALGPYHHFSQELYDNMERYKLMIANQIRHQIEFQSFVCRLSQLDLHI 126

Query: 121 RGSYHRRLDLDADTLALIMAIDALFLLELLYSQVQNHDLLRPLNKFITVSRCKRLSNDAV 180
           RGSYHRRLDLDADTLALIMAIDALFLLELLYSQVQNHDLLRPLNKFITVSRCKRLSNDAV
Sbjct: 127 RGSYHRRLDLDADTLALIMAIDALFLLELLYSQVQNHDLLRPLNKFITVSRCKRLSNDAV 186

Query: 181 VRDIVKLENQIPLFVLREIFAAVIQCDATESDDLLASVLVGFCVEISPLNLTVGRRGVID 240
           VRDIVKLENQIPLFVLREIFAA IQC+ATESDDLLASVLVGFCVEISPLNLTVGRRGVI+
Sbjct: 187 VRDIVKLENQIPLFVLREIFAAAIQCEATESDDLLASVLVGFCVEISPLNLTVGRRGVIE 246

Query: 241 CAHVLDVLYRLILPENLECSGEDDGPKEQPGNIQQSRSLKGNYLWSYPWNWMLDCFKILR 300
           CAHVLDVLYRLILPENLECSGEDDGPKEQPGNIQQSRSLKGNYLWSYPWNWMLDCFKILR
Sbjct: 247 CAHVLDVLYRLILPENLECSGEDDGPKEQPGNIQQSRSLKGNYLWSYPWNWMLDCFKILR 306

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 307 GSKKVFEFIGRLLDLLPIVGGLVSSMEENPDRNRVEILIEDKTPLMEAGIKIPTASQLLD 366

Query: 361 ------------------ESVTLFLPVIKLGANSEVILRNLVAYEAMAMPDILAFSRYLH 420
                             ESVTLFLPVIKLGANSEVILRNLVAYEAMAMPD LAFSRYLH
Sbjct: 367 TGVSFKQSASIKTIKFEAESVTLFLPVIKLGANSEVILRNLVAYEAMAMPDFLAFSRYLH 426

Query: 421 LMNSLIDTAEDVKILKDAEIVVMNGMKKDEEVAVLFNGIMTSSSMGLSAAKELDEAINGV 467
           LMNSLIDTAEDVKILKDAEIVVMNGMKKDEEVAVLFNGIMTSSSMGLSAAKELDEAINGV
Sbjct: 427 LMNSLIDTAEDVKILKDAEIVVMNGMKKDEEVAVLFNGIMTSSSMGLSAAKELDEAINGV 486

BLAST of Carg07730 vs. NCBI nr
Match: XP_023520065.1 (putative UPF0481 protein At3g02645 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 874.8 bits (2259), Expect = 3.4e-250
Identity = 460/544 (84.56%), Postives = 464/544 (85.29%), Query Frame = 0

Query: 1   MNNAMKSLDEGNPCSSSPSILRSKSGERRWVVYINDIIHKHLKNPKSDISSSIFIVPKFL 60
           MNNAMKSLDEGNPCSSSPSILRSKSGERRWVVYINDIIHKHLKNPKSDISSSIFIVPKFL
Sbjct: 7   MNNAMKSLDEGNPCSSSPSILRSKSGERRWVVYINDIIHKHLKNPKSDISSSIFIVPKFL 66

Query: 61  SAAKPQHYTPQFLALGPYHHFSQELYDNMERYKLMIANQIRHQIEFQSFVCRLSQLDLHI 120
           SAAKPQHYTPQFLALGPYHHFSQELYDNMERYKLMIANQIRHQIEFQSFVCRLSQLDLHI
Sbjct: 67  SAAKPQHYTPQFLALGPYHHFSQELYDNMERYKLMIANQIRHQIEFQSFVCRLSQLDLHI 126

Query: 121 RGSYHRRLDLDADTLALIMAIDALFLLELLYSQVQNHDLLRPLNKFITVSRCKRLSNDAV 180
           RGSYHRRLDLDADTLALIMAIDALFLLELLYSQVQNHDLLRPLNKFITVSRCKRLSNDAV
Sbjct: 127 RGSYHRRLDLDADTLALIMAIDALFLLELLYSQVQNHDLLRPLNKFITVSRCKRLSNDAV 186

Query: 181 VRDIVKLENQIPLFVLREIFAAVIQCDATESDDLLASVLVGFCVEISPLNLTVGRRGVID 240
           VRDIVKLENQIPLFVLREIFAA IQC+ATESDDLLASVLVGFC+EISPLNLTVGRRGVI+
Sbjct: 187 VRDIVKLENQIPLFVLREIFAAAIQCEATESDDLLASVLVGFCIEISPLNLTVGRRGVIE 246

Query: 241 CAHVLDVLYRLILPENLECSGEDDGPKEQPGNIQQSRSLKGNYLWSYPWNWMLDCFKILR 300
           CAHVLDVLYRLILPENLECSGEDDGPKEQPGNIQQSRSLKGNYLWSYPWNWMLDCFKILR
Sbjct: 247 CAHVLDVLYRLILPENLECSGEDDGPKEQPGNIQQSRSLKGNYLWSYPWNWMLDCFKILR 306

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 307 GSKKVFEFIGRLLDLLPIVGGLVSSMEENPDRNRVEILIEDKTPLMEAGIKIPTASQLLD 366

Query: 361 ------------------ESVTLFLPVIKLGANSEVILRNLVAYEAMAMPDILAFSRYLH 420
                             ESVTLFLPVIKLGANSEVILRNLVAYEAMAMPDILAFSRYLH
Sbjct: 367 TGVSFKQSASIKTIKFEAESVTLFLPVIKLGANSEVILRNLVAYEAMAMPDILAFSRYLH 426

Query: 421 LMNSLIDTAEDVKILKDAEIVVMNGMKKDEEVAVLFNGIMTSSSMGLSAAKELDEAINGV 467
           LMNSLIDTAEDVKILKDAEIVVMNGMKKDEEVAVLFNGIMTSSSMGLSAAKELDEAINGV
Sbjct: 427 LMNSLIDTAEDVKILKDAEIVVMNGMKKDEEVAVLFNGIMTSSSMGLSAAKELDEAINGV 486

BLAST of Carg07730 vs. NCBI nr
Match: XP_023000998.1 (putative UPF0481 protein At3g02645 [Cucurbita maxima])

HSP 1 Score: 864.4 bits (2232), Expect = 4.5e-247
Identity = 453/544 (83.27%), Postives = 461/544 (84.74%), Query Frame = 0

Query: 1   MNNAMKSLDEGNPCSSSPSILRSKSGERRWVVYINDIIHKHLKNPKSDISSSIFIVPKFL 60
           MNNAMK LDEG+PCSSSPSILRSKSGERRWVVYINDII+KHLKNPKSDISSSIFIVPKFL
Sbjct: 7   MNNAMKPLDEGSPCSSSPSILRSKSGERRWVVYINDIIYKHLKNPKSDISSSIFIVPKFL 66

Query: 61  SAAKPQHYTPQFLALGPYHHFSQELYDNMERYKLMIANQIRHQIEFQSFVCRLSQLDLHI 120
           SAAKPQHYTPQFLALGPYHHFSQELYDNMERYKLMIANQIRHQIEFQSFVCRLSQLDLHI
Sbjct: 67  SAAKPQHYTPQFLALGPYHHFSQELYDNMERYKLMIANQIRHQIEFQSFVCRLSQLDLHI 126

Query: 121 RGSYHRRLDLDADTLALIMAIDALFLLELLYSQVQNHDLLRPLNKFITVSRCKRLSNDAV 180
           RGSYHRRLDLDADTLALIMAIDALFLLELLYSQVQNHDLLRPLNKFITVSRCKRLSNDAV
Sbjct: 127 RGSYHRRLDLDADTLALIMAIDALFLLELLYSQVQNHDLLRPLNKFITVSRCKRLSNDAV 186

Query: 181 VRDIVKLENQIPLFVLREIFAAVIQCDATESDDLLASVLVGFCVEISPLNLTVGRRGVID 240
           VRDIVKLENQIPLFVLREIFAA IQC+ATESDDLLASVLVGFC+EISPLNLTVGRRGVID
Sbjct: 187 VRDIVKLENQIPLFVLREIFAAAIQCEATESDDLLASVLVGFCIEISPLNLTVGRRGVID 246

Query: 241 CAHVLDVLYRLILPENLECSGEDDGPKEQPGNIQQSRSLKGNYLWSYPWNWMLDCFKILR 300
           CAHVLD+LYRLILPENLECSGEDDGPKEQPGNIQ SRSLKGNYLWSYPWNWMLDCFKILR
Sbjct: 247 CAHVLDILYRLILPENLECSGEDDGPKEQPGNIQHSRSLKGNYLWSYPWNWMLDCFKILR 306

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 307 GSKKVFEFIGRLLDLLPIVGGLVSSMEENPDRNRVEILIEDKTPLMEAGIKIPTASQLFD 366

Query: 361 ------------------ESVTLFLPVIKLGANSEVILRNLVAYEAMAMPDILAFSRYLH 420
                             ESVTLFLPVIKLGANSEVILRNLVAYEAMAMPD LAFSRY+H
Sbjct: 367 TGVSFKQSASIKTIKFEAESVTLFLPVIKLGANSEVILRNLVAYEAMAMPDFLAFSRYMH 426

Query: 421 LMNSLIDTAEDVKILKDAEIVVMNGMKKDEEVAVLFNGIMTSSSMGLSAAKELDEAINGV 467
           LMNSLIDTAEDVKILKDAEIVVMNGMKKDEEVAVLFNGIMT+SSMGLSAAKELDEAINGV
Sbjct: 427 LMNSLIDTAEDVKILKDAEIVVMNGMKKDEEVAVLFNGIMTNSSMGLSAAKELDEAINGV 486

BLAST of Carg07730 vs. NCBI nr
Match: KAG6584271.1 (putative UPF0481 protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 834.7 bits (2155), Expect = 3.9e-238
Identity = 434/456 (95.18%), Postives = 439/456 (96.27%), Query Frame = 0

Query: 1   MNNAMKSLDEGNPCSSSPSILRSKSGERRWVVYINDIIHKHLKNPKSDISSSIFIVPKFL 60
           MNNAMKSLDEGNPCSSSPSILRSKSGERRWVVYINDIIHKHLKNPKSDISSSIFIVPKFL
Sbjct: 1   MNNAMKSLDEGNPCSSSPSILRSKSGERRWVVYINDIIHKHLKNPKSDISSSIFIVPKFL 60

Query: 61  SAAKPQHYTPQFLALGPYHHFSQELYDNMERYKLMIANQIRHQIEFQSFVCRLSQLDLHI 120
           SAAKPQHYTPQFLALGPYHHFSQELYDNMERYKLMIANQIRHQIEFQSFVCRLSQLDLHI
Sbjct: 61  SAAKPQHYTPQFLALGPYHHFSQELYDNMERYKLMIANQIRHQIEFQSFVCRLSQLDLHI 120

Query: 121 RGSYHRRLDLDADTLALIMAIDALFLLELLYSQVQNHDLLRPLNKFITVSRCKRLSNDAV 180
           RGSYHRRLDLDADTLALIMAIDALFLLELLYSQVQNHDLL PLNKFITVSRCKRLSNDAV
Sbjct: 121 RGSYHRRLDLDADTLALIMAIDALFLLELLYSQVQNHDLLCPLNKFITVSRCKRLSNDAV 180

Query: 181 VRDIVKLENQIPLFVLREIFAAVIQCDATESDDLLASVLVGFCVEISPLNLTVGRRGVID 240
           VRDIVKLENQIPLFVLREIFAA IQC+ATESDDLLASVLVGFCVEISPLNLTVGRRGVI+
Sbjct: 181 VRDIVKLENQIPLFVLREIFAAAIQCEATESDDLLASVLVGFCVEISPLNLTVGRRGVIE 240

Query: 241 CAHVLDVLYRLILPENLECSGEDDGPKEQPGNIQQSRSLKGNYLWSYPWNWMLDCFKILR 300
           CAHVLDVLYRLILPENLECSGEDDGPKEQPGNIQQSRSLKG    S+  +  +   K   
Sbjct: 241 CAHVLDVLYRLILPENLECSGEDDGPKEQPGNIQQSRSLKG---VSFKQSASIKTIKFEA 300

Query: 301 ESVTLFLPVIKLGANSEVILRNLVAYEAMAMPDILAFSRYLHLMNSLIDTAEDVKILKDA 360
           ESVTLFLPVIKLGANSEVILRNLVAYEAMAMPD LAFSRYLHLMNSLIDTAEDVKILKDA
Sbjct: 301 ESVTLFLPVIKLGANSEVILRNLVAYEAMAMPDFLAFSRYLHLMNSLIDTAEDVKILKDA 360

Query: 361 EIVVMNGMKKDEEVAVLFNGIMTSSSMGLSAAKELDEAINGVNKYYKGRPKVKASRVIKK 420
           EIVVMNGMKKDEEVAVLFNGIMTSSSMGLSAAKELDEAINGVNKYYKGRPKVKASRVIKK
Sbjct: 361 EIVVMNGMKKDEEVAVLFNGIMTSSSMGLSAAKELDEAINGVNKYYKGRPKVKASRVIKK 420

Query: 421 YVYSSWRILALMATLMVLGLLVLQSFCSVYNCPRLF 457
           YVYSSWRILALMATLMVLGLLVLQSFCSVYNCPRLF
Sbjct: 421 YVYSSWRILALMATLMVLGLLVLQSFCSVYNCPRLF 453

BLAST of Carg07730 vs. ExPASy Swiss-Prot
Match: P0C897 (Putative UPF0481 protein At3g02645 OS=Arabidopsis thaliana OX=3702 GN=At3g02645 PE=3 SV=1)

HSP 1 Score: 234.6 bits (597), Expect = 2.3e-60
Identity = 176/522 (33.72%), Postives = 269/522 (51.53%), Query Frame = 0

Query: 27  ERRWVVYINDIIHKHL-KNPKSDISSSIFIVPKFLSAAKPQHYTPQFLALGPYHHFSQEL 86
           E RWV+ +   +   L ++   +++ SIF VPK L  + P  YTP  +++GPYH    EL
Sbjct: 18  ETRWVINVQKSLDAELEEHDLEEVTVSIFNVPKALMCSHPDSYTPHRVSIGPYHCLKPEL 77

Query: 87  YDNMERYKLMIANQIRHQ---IEFQSFVCRLSQLDLHIRGSYHRRLDLDADTLALIMAID 146
           ++ MERYKLMIA +IR+Q     F   V +L  +++ IR  YH+ +  + +TL  IMA+D
Sbjct: 78  HE-MERYKLMIARKIRNQYNSFRFHDLVEKLQSMEIKIRACYHKYIGFNGETLLWIMAVD 137

Query: 147 ALFLLELL--YSQVQNHDLLRPLNKFITVSRCKRLSNDAVVRDIVKLENQIPLFVLREIF 206
           + FL+E L  YS        R +   I      R+ ++ ++RDI+ +ENQIPLFVLR+  
Sbjct: 138 SSFLIEFLKIYS-------FRKVETLI-----NRVGHNEILRDIMMIENQIPLFVLRKTL 197

Query: 207 AAVIQCDATES-DDLLASVLVGFCVEISPLNLTVGRRGVI-----DCAHVLDVLYRLILP 266
               Q ++TES DDLL SVL G C ++SPL +      ++     +C H+LD LY++I+P
Sbjct: 198 E--FQLESTESADDLLLSVLTGLCKDLSPLVIKFDDDQILKAQFQECNHILDFLYQMIVP 257

Query: 267 ----ENLECSGEDDGPKEQPGN--IQQSRSLKGNY-----------LWSYPWNWM--LDC 326
               E LE   E++   E  GN  I+    +K  +           +  +PW  +  L  
Sbjct: 258 RIEEEELEEDDEENRADENGGNRAIRFMDEIKHQFKRVFASRPADLILRFPWRIISNLPG 317

Query: 327 FKILR-------------------ESVTL------------------------------- 386
           F  L+                   ESV++                               
Sbjct: 318 FMALKLSADYLFTRQENEATTTRQESVSILDIEKPPLVEELTIPSVSDLHKAGVRFKPTA 377

Query: 387 ----------------FLPVIKLGANSEVILRNLVAYEAMAMPDILAFSRYLHLMNSLID 446
                           +LPVI L  N+E +LRNLVAYEA      L F+RY  L+N +ID
Sbjct: 378 HGNISTVTFDSNSGQFYLPVINLDINTETVLRNLVAYEATNTSGPLVFTRYTELINGIID 437

Query: 447 TAEDVKILKDAEIVVMNGMKKDEEVAVLFNGIMTSSSMGLSAAKELDEAINGVNKYYKGR 452
           + EDV++L++  ++V + +K D+E A ++NG+  S S+ L+    LD+ I  VN+YY GR
Sbjct: 438 SEEDVRLLREQGVLV-SRLKSDQEAAEMWNGM--SKSVRLTKVGFLDKTIEDVNRYYTGR 497

BLAST of Carg07730 vs. ExPASy TrEMBL
Match: A0A6J1E7C3 (putative UPF0481 protein At3g02645 OS=Cucurbita moschata OX=3662 GN=LOC111431467 PE=4 SV=1)

HSP 1 Score: 877.1 bits (2265), Expect = 3.3e-251
Identity = 462/544 (84.93%), Postives = 464/544 (85.29%), Query Frame = 0

Query: 1   MNNAMKSLDEGNPCSSSPSILRSKSGERRWVVYINDIIHKHLKNPKSDISSSIFIVPKFL 60
           MNNAMKSLDEGNPCSSSPSILRSKSGERRWVVYINDIIHKHLKNPKSDISSSIFIVPKFL
Sbjct: 7   MNNAMKSLDEGNPCSSSPSILRSKSGERRWVVYINDIIHKHLKNPKSDISSSIFIVPKFL 66

Query: 61  SAAKPQHYTPQFLALGPYHHFSQELYDNMERYKLMIANQIRHQIEFQSFVCRLSQLDLHI 120
           SAAKPQHYTPQFLALGPYHHFSQELYDNMERYKLMIANQIRHQIEFQSFVCRLSQLDLHI
Sbjct: 67  SAAKPQHYTPQFLALGPYHHFSQELYDNMERYKLMIANQIRHQIEFQSFVCRLSQLDLHI 126

Query: 121 RGSYHRRLDLDADTLALIMAIDALFLLELLYSQVQNHDLLRPLNKFITVSRCKRLSNDAV 180
           RGSYHRRLDLDADTLALIMAIDALFLLELLYSQVQNHDLLRPLNKFITVSRCKRLSNDAV
Sbjct: 127 RGSYHRRLDLDADTLALIMAIDALFLLELLYSQVQNHDLLRPLNKFITVSRCKRLSNDAV 186

Query: 181 VRDIVKLENQIPLFVLREIFAAVIQCDATESDDLLASVLVGFCVEISPLNLTVGRRGVID 240
           VRDIVKLENQIPLFVLREIFAA IQC+ATESDDLLASVLVGFCVEISPLNLTVGRRGVI+
Sbjct: 187 VRDIVKLENQIPLFVLREIFAAAIQCEATESDDLLASVLVGFCVEISPLNLTVGRRGVIE 246

Query: 241 CAHVLDVLYRLILPENLECSGEDDGPKEQPGNIQQSRSLKGNYLWSYPWNWMLDCFKILR 300
           CAHVLDVLYRLILPENLECSGEDDGPKEQPGNIQQSRSLKGNYLWSYPWNWMLDCFKILR
Sbjct: 247 CAHVLDVLYRLILPENLECSGEDDGPKEQPGNIQQSRSLKGNYLWSYPWNWMLDCFKILR 306

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 307 GSKKVFEFIGRLLDLLPIVGGLVSSMEENPDRNRVEILIEDKTPLMEAGIKIPTASQLLD 366

Query: 361 ------------------ESVTLFLPVIKLGANSEVILRNLVAYEAMAMPDILAFSRYLH 420
                             ESVTLFLPVIKLGANSEVILRNLVAYEAMAMPD LAFSRYLH
Sbjct: 367 TGVSFKQSASIKTIKFEAESVTLFLPVIKLGANSEVILRNLVAYEAMAMPDFLAFSRYLH 426

Query: 421 LMNSLIDTAEDVKILKDAEIVVMNGMKKDEEVAVLFNGIMTSSSMGLSAAKELDEAINGV 467
           LMNSLIDTAEDVKILKDAEIVVMNGMKKDEEVAVLFNGIMTSSSMGLSAAKELDEAINGV
Sbjct: 427 LMNSLIDTAEDVKILKDAEIVVMNGMKKDEEVAVLFNGIMTSSSMGLSAAKELDEAINGV 486

BLAST of Carg07730 vs. ExPASy TrEMBL
Match: A0A6J1KP86 (putative UPF0481 protein At3g02645 OS=Cucurbita maxima OX=3661 GN=LOC111495266 PE=4 SV=1)

HSP 1 Score: 864.4 bits (2232), Expect = 2.2e-247
Identity = 453/544 (83.27%), Postives = 461/544 (84.74%), Query Frame = 0

Query: 1   MNNAMKSLDEGNPCSSSPSILRSKSGERRWVVYINDIIHKHLKNPKSDISSSIFIVPKFL 60
           MNNAMK LDEG+PCSSSPSILRSKSGERRWVVYINDII+KHLKNPKSDISSSIFIVPKFL
Sbjct: 7   MNNAMKPLDEGSPCSSSPSILRSKSGERRWVVYINDIIYKHLKNPKSDISSSIFIVPKFL 66

Query: 61  SAAKPQHYTPQFLALGPYHHFSQELYDNMERYKLMIANQIRHQIEFQSFVCRLSQLDLHI 120
           SAAKPQHYTPQFLALGPYHHFSQELYDNMERYKLMIANQIRHQIEFQSFVCRLSQLDLHI
Sbjct: 67  SAAKPQHYTPQFLALGPYHHFSQELYDNMERYKLMIANQIRHQIEFQSFVCRLSQLDLHI 126

Query: 121 RGSYHRRLDLDADTLALIMAIDALFLLELLYSQVQNHDLLRPLNKFITVSRCKRLSNDAV 180
           RGSYHRRLDLDADTLALIMAIDALFLLELLYSQVQNHDLLRPLNKFITVSRCKRLSNDAV
Sbjct: 127 RGSYHRRLDLDADTLALIMAIDALFLLELLYSQVQNHDLLRPLNKFITVSRCKRLSNDAV 186

Query: 181 VRDIVKLENQIPLFVLREIFAAVIQCDATESDDLLASVLVGFCVEISPLNLTVGRRGVID 240
           VRDIVKLENQIPLFVLREIFAA IQC+ATESDDLLASVLVGFC+EISPLNLTVGRRGVID
Sbjct: 187 VRDIVKLENQIPLFVLREIFAAAIQCEATESDDLLASVLVGFCIEISPLNLTVGRRGVID 246

Query: 241 CAHVLDVLYRLILPENLECSGEDDGPKEQPGNIQQSRSLKGNYLWSYPWNWMLDCFKILR 300
           CAHVLD+LYRLILPENLECSGEDDGPKEQPGNIQ SRSLKGNYLWSYPWNWMLDCFKILR
Sbjct: 247 CAHVLDILYRLILPENLECSGEDDGPKEQPGNIQHSRSLKGNYLWSYPWNWMLDCFKILR 306

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 307 GSKKVFEFIGRLLDLLPIVGGLVSSMEENPDRNRVEILIEDKTPLMEAGIKIPTASQLFD 366

Query: 361 ------------------ESVTLFLPVIKLGANSEVILRNLVAYEAMAMPDILAFSRYLH 420
                             ESVTLFLPVIKLGANSEVILRNLVAYEAMAMPD LAFSRY+H
Sbjct: 367 TGVSFKQSASIKTIKFEAESVTLFLPVIKLGANSEVILRNLVAYEAMAMPDFLAFSRYMH 426

Query: 421 LMNSLIDTAEDVKILKDAEIVVMNGMKKDEEVAVLFNGIMTSSSMGLSAAKELDEAINGV 467
           LMNSLIDTAEDVKILKDAEIVVMNGMKKDEEVAVLFNGIMT+SSMGLSAAKELDEAINGV
Sbjct: 427 LMNSLIDTAEDVKILKDAEIVVMNGMKKDEEVAVLFNGIMTNSSMGLSAAKELDEAINGV 486

BLAST of Carg07730 vs. ExPASy TrEMBL
Match: A0A2R6QS22 (UPF0481 protein OS=Actinidia chinensis var. chinensis OX=1590841 GN=CEY00_Acc14518 PE=4 SV=1)

HSP 1 Score: 324.7 bits (831), Expect = 6.3e-85
Identity = 206/501 (41.12%), Postives = 296/501 (59.08%), Query Frame = 0

Query: 19  SILRSKSGERRWVVYINDIIHKHLKNPKSDISSSIFIVPKFLSAAKPQHYTPQFLALGPY 78
           SI  S S ERRW+  I+ ++ K ++   +D+  SIF VP  LS+ KP+ YTPQ + LGPY
Sbjct: 5   SIFESSSSERRWINQISKMLEKEVRIDINDVPVSIFRVPTTLSSFKPEAYTPQLIGLGPY 64

Query: 79  HHFSQELYDNMERYKLMIANQIRHQ---IEFQSFVCRLSQLDLHIRGSYHRRLDLDADTL 138
           H F  ELY+ MERYK+  A+++++Q   +EF+  V +L++++  +R SYH+ LD+D +TL
Sbjct: 65  HRFRPELYE-MERYKIAAASRVQNQFQSLEFKQLVDKLNEIEYKVRASYHKYLDIDGETL 124

Query: 139 ALIMAIDALFLLELLYSQVQNHDLLRPLN--KFITVSRCKRLSNDAVVRDIVKLENQIPL 198
           A IMAID LFLL+ L S V   D L  L+   F+     K+L+NDA++ DIV LENQ+P+
Sbjct: 125 AWIMAIDGLFLLDFLNSYVNKKDSLSSLSTTAFLVDGSGKKLANDAILGDIVMLENQMPI 184

Query: 199 FVLREIFAAVIQCDATE-SDDLLASVLVGFCVEISPLNL--TVGRRGVIDCAHVLDVLYR 258
           F+L EI    IQ  + +   +LL  +L+GFC  +SPL L   +    V D AH+LD+LY 
Sbjct: 185 FLLSEIL--TIQSSSPDLGKNLLPPMLMGFCKAVSPLKLKEDLPLSQVFDHAHLLDLLYH 244

Query: 259 LILPENLECSGEDDGPK---------------------EQPG----------NIQQSR-- 318
           L +P+  E    D+G K                       PG          NI++ R  
Sbjct: 245 LTVPKLEEHHETDNGEKGGASLGGKTSTDSGLPWKFITSLPGISNFTSKAKENIKRDREN 304

Query: 319 ----SLKG-----------NYLWS-----YPWNWMLDCFKILRESVTLFLPVIKLGANSE 378
                +KG           +YL+      YP    +   K  +++ T +LPVI L  NSE
Sbjct: 305 LSGQEMKGPLVEEIMIPSVSYLFDAGVQFYPTAGDITTIKFDQKTKTFYLPVITLNVNSE 364

Query: 379 VILRNLVAYEAMAMPDILAFSRYLHLMNSLIDTAEDVKILKDAEIVVMNGMKKDEEVAVL 438
           VI+RNLVAYEA    + L  +RY  L+N +IDT ED K+L++ +I++ N +K D EVA L
Sbjct: 365 VIMRNLVAYEASIASESLVLARYTELLNGIIDTDEDAKLLREKKIII-NSLKSDAEVAEL 424

Query: 439 FNGIMTSSSMGLSAAKELDEAINGVNKYYKGRPKVKASRVIKKYVYSSWRILALMATLMV 459
           FNG+  S S+ L+    +DEAI+GVNKY+    KV+    +KKYVYSSW+IL L+A +M+
Sbjct: 425 FNGM--SKSVRLTNVSFIDEAIDGVNKYFYNTKKVRTYNRMKKYVYSSWKILTLIAVVML 484

BLAST of Carg07730 vs. ExPASy TrEMBL
Match: A0A7J0FLR8 (Transmembrane protein, putative OS=Actinidia rufa OX=165716 GN=Acr_13g0010710 PE=4 SV=1)

HSP 1 Score: 285.4 bits (729), Expect = 4.2e-73
Identity = 195/504 (38.69%), Postives = 286/504 (56.75%), Query Frame = 0

Query: 19  SILRSKSGERRWVVYINDIIHKHLKNPKSDISSSIFIV---PKFLSAAKPQHYTPQFLAL 78
           SI  S S ERRW+  I+ ++ K ++   +D+  SIF      +F  A      T +F   
Sbjct: 5   SIFESSSSERRWINQISKMLEKEVRIEINDVPVSIFSCAYNSEFFQARSLYASTHRF--- 64

Query: 79  GPYHHFSQELYDNMERYKLMIANQIRHQ---IEFQSFVCRLSQLDLHIRGSYHRRLDLDA 138
           G    FS ELY+ MERYK+  A+++++Q   +EF+  V +L++++  +R SYH+ LD+D 
Sbjct: 65  GALPSFSPELYE-MERYKIAAASRVQNQFQSLEFKQLVDKLNEIEYKVRASYHKYLDIDG 124

Query: 139 DTLALIMAIDALFLLELLYSQVQNHDLLRPLN--KFITVSRCKRLSNDAVVRDIVKLENQ 198
           +TLA IMAID LFLL+ L S V   D L  L+   F+     K+L+NDA++ DIV LENQ
Sbjct: 125 ETLAWIMAIDGLFLLDFLNSYVNKRDSLSSLSTTAFLVDGSGKKLANDAILGDIVMLENQ 184

Query: 199 IPLFVLREIFAAVIQCDATE-SDDLLASVLVGFCVEISPLNL--TVGRRGVIDCAHVLDV 258
           +P+F+L EI    IQ  + +   +LL  +L+GFC  +SPL L   +    V D AH+LD+
Sbjct: 185 MPIFLLSEIL--TIQSSSPDLGKNLLPPMLMGFCKAVSPLKLKEDLPLSQVFDHAHLLDL 244

Query: 259 LYRLILPENLECSGEDDGPK---------------------EQPG----------NIQQS 318
           LY L +P+  E    D+G K                       PG          NI++ 
Sbjct: 245 LYHLTVPKLEEHHETDNGEKGGASLGGKTSTDSGLPWKFITSLPGISNFTSKAKENIKRD 304

Query: 319 R------SLKG-----------NYLWS-----YPWNWMLDCFKILRESVTLFLPVIKLGA 378
           R       +KG           +YL+      YP    +   K  +++ T +LPVI L  
Sbjct: 305 RENLSGQEMKGPLVEEIMIPSVSYLFDAGVEFYPTAGDITTIKFDQKTKTFYLPVITLNV 364

Query: 379 NSEVILRNLVAYEAMAMPDILAFSRYLHLMNSLIDTAEDVKILKDAEIVVMNGMKKDEEV 438
           NSEVI+RNLVAYEA    + L  +RY  L+N +IDT +D K+L++ +I++ N +K D +V
Sbjct: 365 NSEVIMRNLVAYEASIASESLVLARYTELLNGIIDTDKDAKLLREKKIII-NSLKSDADV 424

Query: 439 AVLFNGIMTSSSMGLSAAKELDEAINGVNKYYKGRPKVKASRVIKKYVYSSWRILALMAT 459
           A LFNG+  S S+ L+    +DEAI+GVNKY+    KV+    +KKYVYSSW+IL L+A 
Sbjct: 425 AELFNGM--SKSVRLTNVSFIDEAIDGVNKYFYNTKKVRTYNRMKKYVYSSWKILTLIAV 484

BLAST of Carg07730 vs. ExPASy TrEMBL
Match: A0A7J7H0H1 (Uncharacterized protein OS=Camellia sinensis OX=4442 GN=HYC85_015366 PE=4 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 8.8e-71
Identity = 193/541 (35.67%), Postives = 285/541 (52.68%), Query Frame = 0

Query: 19  SILRSKSGERRWVVYINDIIHKHLKNPKSDISSSIFIVPKFLSAAKPQHYTPQFLALGPY 78
           SI  S  GE+RW+  ++ I+       + D+  SIF VP  +SA KP+ YTPQ + LGPY
Sbjct: 5   SIFESSEGEQRWISQVSKILQNDEVMVEIDLPVSIFRVPTTISAFKPEAYTPQLMGLGPY 64

Query: 79  HHFSQELYDNMERYKLMIANQIRHQ---IEFQSFVCRLSQLDLHIRGSYHRRLDLDADTL 138
           HHF  ELY+ MERYKL  A++++ Q   +EF+  V +L++ +  +R  YH+ LD++ DTL
Sbjct: 65  HHFRSELYE-MERYKLAAASRLQKQFKSLEFKQLVEKLAKFEHKVRACYHKYLDVECDTL 124

Query: 139 ALIMAIDALFLLELLYS---QVQNHDLLRPLNKFITVSRCKRLSNDAVVRDIVKLENQIP 198
           A IMAID LFLL+ L +    V+ +         +  S  ++L++ +++ DI+ LENQIP
Sbjct: 125 AWIMAIDGLFLLDFLQNYLIHVKENISSTSTTAHLVDSSGRKLAHGSILGDIMMLENQIP 184

Query: 199 LFVLREIFAAVIQCDATE-SDDLLASVLVGFCVEISPLNLT---VGRRGVIDCAHVLDVL 258
           +FVLR I +  IQC   +  D+LL ++L+GFC  +SPL L    +    V + AH+LD+L
Sbjct: 185 IFVLRAILS--IQCSLPDVEDNLLPTMLMGFCKALSPLKLVKEDLPLSQVSEHAHLLDLL 244

Query: 259 YRLILPENLECSGEDDGPKEQPGNIQQSRSLKG------------NYLWSYPWNW----- 318
           Y L +P+ LE S E  G K   G  + S S               + LWS   N      
Sbjct: 245 YHLTVPK-LENSQEHQG-KNTCGKFESSSSHAAEKFDSSKSCHVFDKLWSLLSNLNVGFV 304

Query: 319 ------------------------------------------------------MLDCFK 378
                                                                 M+    
Sbjct: 305 RKITKPIQNILGVSSKIVSSVPGISFLSSKKKEDIKPEDEASSDEKKTPKVEEIMIPSVS 364

Query: 379 ILRESVTLF--------------------LPVIKLGANSEVILRNLVAYEAMAMPDILAF 438
           +LR +   F                    LPVI L  NSEV++RNLVAYEA    + L F
Sbjct: 365 MLRFAGVEFCSSNGGLATIKFNKYTKKFHLPVIILNVNSEVVMRNLVAYEASTTSESLVF 424

Query: 439 SRYLHLMNSLIDTAEDVKILKDAEIVVMNGMKKDEEVAVLFNGIMTSSSMGLSAAKELDE 459
           +RY+ LMN +IDTAED K+L++ +I++ N +K D +VA LFNG+  S S+ L+    +D+
Sbjct: 425 ARYMELMNGIIDTAEDAKMLRENKIII-NSLKSDADVAELFNGM--SKSVRLTNVPYIDK 484

BLAST of Carg07730 vs. TAIR 10
Match: AT3G02645.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 234.6 bits (597), Expect = 1.6e-61
Identity = 176/522 (33.72%), Postives = 269/522 (51.53%), Query Frame = 0

Query: 27  ERRWVVYINDIIHKHL-KNPKSDISSSIFIVPKFLSAAKPQHYTPQFLALGPYHHFSQEL 86
           E RWV+ +   +   L ++   +++ SIF VPK L  + P  YTP  +++GPYH    EL
Sbjct: 18  ETRWVINVQKSLDAELEEHDLEEVTVSIFNVPKALMCSHPDSYTPHRVSIGPYHCLKPEL 77

Query: 87  YDNMERYKLMIANQIRHQ---IEFQSFVCRLSQLDLHIRGSYHRRLDLDADTLALIMAID 146
           ++ MERYKLMIA +IR+Q     F   V +L  +++ IR  YH+ +  + +TL  IMA+D
Sbjct: 78  HE-MERYKLMIARKIRNQYNSFRFHDLVEKLQSMEIKIRACYHKYIGFNGETLLWIMAVD 137

Query: 147 ALFLLELL--YSQVQNHDLLRPLNKFITVSRCKRLSNDAVVRDIVKLENQIPLFVLREIF 206
           + FL+E L  YS        R +   I      R+ ++ ++RDI+ +ENQIPLFVLR+  
Sbjct: 138 SSFLIEFLKIYS-------FRKVETLI-----NRVGHNEILRDIMMIENQIPLFVLRKTL 197

Query: 207 AAVIQCDATES-DDLLASVLVGFCVEISPLNLTVGRRGVI-----DCAHVLDVLYRLILP 266
               Q ++TES DDLL SVL G C ++SPL +      ++     +C H+LD LY++I+P
Sbjct: 198 E--FQLESTESADDLLLSVLTGLCKDLSPLVIKFDDDQILKAQFQECNHILDFLYQMIVP 257

Query: 267 ----ENLECSGEDDGPKEQPGN--IQQSRSLKGNY-----------LWSYPWNWM--LDC 326
               E LE   E++   E  GN  I+    +K  +           +  +PW  +  L  
Sbjct: 258 RIEEEELEEDDEENRADENGGNRAIRFMDEIKHQFKRVFASRPADLILRFPWRIISNLPG 317

Query: 327 FKILR-------------------ESVTL------------------------------- 386
           F  L+                   ESV++                               
Sbjct: 318 FMALKLSADYLFTRQENEATTTRQESVSILDIEKPPLVEELTIPSVSDLHKAGVRFKPTA 377

Query: 387 ----------------FLPVIKLGANSEVILRNLVAYEAMAMPDILAFSRYLHLMNSLID 446
                           +LPVI L  N+E +LRNLVAYEA      L F+RY  L+N +ID
Sbjct: 378 HGNISTVTFDSNSGQFYLPVINLDINTETVLRNLVAYEATNTSGPLVFTRYTELINGIID 437

Query: 447 TAEDVKILKDAEIVVMNGMKKDEEVAVLFNGIMTSSSMGLSAAKELDEAINGVNKYYKGR 452
           + EDV++L++  ++V + +K D+E A ++NG+  S S+ L+    LD+ I  VN+YY GR
Sbjct: 438 SEEDVRLLREQGVLV-SRLKSDQEAAEMWNGM--SKSVRLTKVGFLDKTIEDVNRYYTGR 497

BLAST of Carg07730 vs. TAIR 10
Match: AT3G50140.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 100.5 bits (249), Expect = 3.7e-21
Identity = 109/460 (23.70%), Postives = 197/460 (42.83%), Query Frame = 0

Query: 30  WVVYINDIIHKHLKNPKSDISSSIFI--VPKFLSAAKPQHYTPQFLALGPYHHFSQELYD 89
           WV++I D + + +++  +     I I  VP  L  +    Y PQ ++LGPYHH  + L  
Sbjct: 90  WVIWIKDKMEQVMRDAATTSWDKICIYRVPLSLKKSDKNSYFPQAVSLGPYHHGDEHLRP 149

Query: 90  NMERYKLMIANQI--RHQIEFQSFVCRLSQLDLHIRGSYHRRLDLDADTLALIMAIDALF 149
            M+ +K    N +  R +   + ++  + +L+   R  Y   + L ++    ++ +D  F
Sbjct: 150 -MDYHKWRAVNMVMKRTKQGIEMYIDAMKELEERARACYEGPIGLSSNKFTQMLVLDGCF 209

Query: 150 LLELLYSQVQNHDLLRPLNKFITVSRCKRLSNDAVVRDIVKLENQIPLFVLREIFAAVIQ 209
           +L+L     +    L         +   R S  ++ RD++ LENQ+PLFVL  +    +Q
Sbjct: 210 VLDLFRGAYEGFSKLGYDRNDPVFA--MRGSMHSIRRDMLMLENQLPLFVLNRLLE--LQ 269

Query: 210 CDATESDDLLASVLVGFCVEISPLNLTVGR------------RGVIDC----AHVLDVLY 269
                   L+A + V F   + P  ++  +              + D      H LDV  
Sbjct: 270 LGTQYQTGLVAQLAVRFFNPLMPTYMSSTKIENSQENNNKFFNPIADKEKEELHCLDVFR 329

Query: 270 RLILPENLECSGEDDGPKEQPGNIQQSRSLKGNYLWSYPWNWMLDCFKILRESVTLF--- 329
           R +L  +L         K  P  + +SR  +   +       +L C   LRE+   F   
Sbjct: 330 RSLLQPSL---------KPDP-RLSRSRWSRKPLVADKRQQQLLHCVTELREAGIKFKRR 389

Query: 330 ----------------LPVIKLGANSEVILRNLVAYEAMAMPDILAFSRYLHLMNSLIDT 389
                           +P + +   ++ +  NL+AYE   +      + Y+  M++LID+
Sbjct: 390 KSDRFWDIQFKNGCLEIPKLLIHDGTKSLFSNLIAYEQCHIDSTNDITSYIIFMDNLIDS 449

Query: 390 AEDVKILKDAEIVVMNGMKKDEEVAVLFNGIMTSSSMGLSAAKELDEAINGVNKYYKGRP 449
           AED++ L   +I + + +  D EVA +FN +    +  L     L E  N V++YY  + 
Sbjct: 450 AEDIRYLHYYDI-IEHWLGNDSEVADVFNRLCQEVAFDLENT-YLSELSNKVDRYYNRKW 509

Query: 450 KVKASRVIKKYVYSSWRILALMATLMVLGLLVLQSFCSVY 451
            V  + +  KY  + W   +  A +++L L + QSF + Y
Sbjct: 510 NVLKATLKHKYFSNPWAYFSFFAAVILLLLTLFQSFFTSY 532

BLAST of Carg07730 vs. TAIR 10
Match: AT3G50150.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 97.8 bits (242), Expect = 2.4e-20
Identity = 110/455 (24.18%), Postives = 201/455 (44.18%), Query Frame = 0

Query: 22  RSKSGERRWVVYINDIIHKHLKNPKSDI--SSSIFIVPKFLSAAKPQHYTPQFLALGPYH 81
           + +     WV+ I D + K L    ++      I+ VP +L     + Y PQ +++GPYH
Sbjct: 59  KPRETREEWVISIKDKMEKALSYDATNSWDKLCIYRVPFYLQENDKKSYLPQTVSIGPYH 118

Query: 82  HFSQELYDNMERYKLMIANQI----RHQIEFQSFVCRLSQLDLHIRGSYHRRLDL-DADT 141
           H    L   MER+K    N I    +H IE   ++  + +L+   R  Y   +D+ +++ 
Sbjct: 119 HGKVHLRP-MERHKWRAVNMIMARTKHNIEM--YIDAMKELEEEARACYQGPIDMKNSNE 178

Query: 142 LALIMAIDALFLLELLYSQVQNHDLLRPLNKFITVSRCKRLSNDAVVRDIVKLENQIPLF 201
              ++ +D  F+LEL    +Q    +         +  KR    ++ RD++ LENQ+PLF
Sbjct: 179 FTEMLVLDGCFVLELFKGTIQGFQKIGYARNDPVFA--KRGLMHSIQRDMIMLENQLPLF 238

Query: 202 VLREIFAAVIQCDATESDDLLASVLVGFCVEISPLN--LTVGRR-----------GVIDC 261
           VL  +    +Q        ++A V V F   + P +  LT   R           G    
Sbjct: 239 VLDRLLG--LQTGTPNQTGIVAEVAVRFFKTLMPTSEVLTKSERSLDSQEKSDELGDNGG 298

Query: 262 AHVLDVLYRLILPENLECSGEDDGPKEQPGNIQQSRSLKG--NYLWSYPWNWML----DC 321
            H LDV +R ++ ++ E + +   P E    +++ + L      L     N+M       
Sbjct: 299 LHCLDVFHRSLI-QSSETTNQGT-PYEDMSMVEKQQQLIHCVTELRGAGVNFMRKETGQL 358

Query: 322 FKILRESVTLFLPVIKLGANSEVILRNLVAYEAMAMPDILAFSRYLHLMNSLIDTAEDVK 381
           + I  ++  L +P + +   ++ +  NL+A+E          + Y+  M++LI++++DV 
Sbjct: 359 WDIEFKNGYLKIPKLLIHDGTKSLFSNLIAFEQCHTQSSNNITSYIIFMDNLINSSQDVS 418

Query: 382 ILKDAEIVVMNGMKKDEEVAVLFNGIMTSSSMGLSAAKELDEAINGVNKYYKGRPKVKAS 441
            L   + ++ + +  D EVA LFN +             L +    VN+YY  +     +
Sbjct: 419 YLHH-DGIIEHWLGSDSEVADLFNRLCKEVIFDPKDG-YLSQLSREVNRYYSRKWNSLKA 478

Query: 442 RVIKKYVYSSWRILALMATLMVLGLLVLQSFCSVY 451
            + +KY  + W   +  A +++L L   QSF +VY
Sbjct: 479 TLRQKYFNNPWAYFSFSAAVILLFLTFFQSFFAVY 502

BLAST of Carg07730 vs. TAIR 10
Match: AT3G50120.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 97.4 bits (241), Expect = 3.1e-20
Identity = 109/468 (23.29%), Postives = 196/468 (41.88%), Query Frame = 0

Query: 24  KSGERRWVVYINDIIHKHLKNPKSDI--SSSIFIVPKFLSAAKPQHYTPQFLALGPYHHF 83
           K     WV+ I D + +  ++  + +     I+ VP +L     + Y PQ ++LGPYHH 
Sbjct: 74  KDSRDDWVISITDKLEQAHRDDDTTLWGKLCIYRVPYYLQENDNKSYFPQTVSLGPYHHG 133

Query: 84  SQELYDNMERYKLMIANQI--RHQIEFQSFVCRLSQLDLHIRGSYHRRLDLDADTLALIM 143
            + L  +M+R+K    N++  R     + ++  + +L+   R  Y   L L ++    ++
Sbjct: 134 KKRL-RSMDRHKWRAVNRVLKRTNQGIKMYIDAMRELEEKARACYEGPLSLSSNEFIEML 193

Query: 144 AIDALFLLELLYSQVQNHDLLRPLNKFITVSRCKRLSNDAVVRDIVKLENQIPLFVLREI 203
            +D  F+LEL    V+    L         +   R S  ++ RD+V LENQ+PLFVL  +
Sbjct: 194 VLDGCFVLELFRGAVEGFTELGYARNDPVFA--MRGSMHSIQRDMVMLENQLPLFVLNRL 253

Query: 204 FAAVIQCDATESDDLLASVLVGFCVEISPLNLTVGRRGV------------------IDC 263
               +Q        L+A + + F   + P +  + + G                   +  
Sbjct: 254 LE--LQLGTRNQTGLVAQLAIRFFDPLMPTDEPLTKSGQSKLENSLARDKSFDPFADMGE 313

Query: 264 AHVLDVLYRLILPENLECSGEDDGPKEQPGNIQQSRSLKGNYLWSYPWNWMLDCFKILRE 323
            H LDV  R +L  +         PK +P  + + R  +   +       ++ C   L+E
Sbjct: 314 LHCLDVFRRSLLRSS---------PKPEP-RLTRKRWSRNTRVADKRRQQLIHCVTELKE 373

Query: 324 SVTLF-------------------LPVIKLGANSEVILRNLVAYEAMAMPDILAFSRYLH 383
           +   F                   +P + +   ++ +  NL+A+E   +      + Y+ 
Sbjct: 374 AGIKFRRRKTDRFWDMQFKNGYLEIPRLLIHDGTKSLFLNLIAFEQCHIDSSNDITSYII 433

Query: 384 LMNSLIDTAEDVKILKDAEIVVMNGMKKDEEVAVLFNGIMTSSSMGLSAAKELDEAINGV 443
            M++LID+ EDV  L    I + + +  D EVA LFN +        +    L      V
Sbjct: 434 FMDNLIDSHEDVSYLHYCGI-IEHWLGSDSEVADLFNRLCQEVVFD-TEDSYLSRLSIEV 493

Query: 444 NKYYKGRPKVKASRVIKKYVYSSWRILALMATLMVLGLLVLQSFCSVY 451
           N+YY  +     + +  KY  + W I++  A +++L L   QSF +VY
Sbjct: 494 NRYYDHKWNAWRATLKHKYFNNPWAIVSFCAAVILLVLTFSQSFYAVY 524

BLAST of Carg07730 vs. TAIR 10
Match: AT2G36430.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 96.3 bits (238), Expect = 7.0e-20
Identity = 109/442 (24.66%), Postives = 195/442 (44.12%), Query Frame = 0

Query: 23  SKSGERRWVVYINDIIHKHLKNPKSDISS-------SIFIVPKFLSAAKPQHYTPQFLAL 82
           +  GE    ++I + +HK LK P   +SS       SIF VP+ +     + Y P+ +++
Sbjct: 11  TSEGESLLKIHI-ERMHKKLKEPPRLLSSAAGKPTCSIFRVPQSMIDCNGRCYEPRVVSI 70

Query: 83  GPYHHFSQELYDNMERYKLMIANQI---RHQIEFQSFVCRLSQLDLHIRGSYHRRLDLDA 142
           GPYH    +L   +E +K    N +      +  + ++  +  ++   R  Y   + +D+
Sbjct: 71  GPYHRGQTQL-KMIEEHKWRYLNVLLTRTQNLTLEDYMKSVKNVEEVARECYSETIHMDS 130

Query: 143 DTLALIMAIDALFLLELLYSQVQNHDLLRPLNKFITVSRCKRLSNDAVVRDIVKLENQIP 202
           +    +M +D  FLLE L+ +V N     P +  + ++           RD + LENQIP
Sbjct: 131 EEFNEMMVLDGCFLLE-LFRKVNNLVPFEPNDPLVAMAWVLPF----FYRDFLCLENQIP 190

Query: 203 LFVLREIFAAVIQCDATESDDLLASVLVGFCVE-ISPLNLTVGRRGVIDCAHVLDVLYRL 262
            FVL  +F      +  E++  L S+   F    +      + R   +   H+LD+L   
Sbjct: 191 FFVLETLFNLTRGDNENETNASLQSLAFAFFNNMMHRTEEDLARFKELRAKHLLDLLRSS 250

Query: 263 ILPEN--LECSGEDDGPKEQPGNIQQSRSLKGNYLWSYPWNWMLDCFKILR-ESVTLFLP 322
            +PE+        + G ++ P +I  S S               + F ++R    T+ +P
Sbjct: 251 FIPESELHTPPATNPGKEKMPSHIIHSISKLRRAGIKLRELKDAESFLVVRFRHGTIEMP 310

Query: 323 VIKLGANSEVILRNLVAYEAMAMPDILAFSRYLHLMNSLIDTAEDVKILKDAEIVVMNGM 382
            I +       L N VAYE   +   + F+ Y  L++ L +T +DV+ L D  I + N  
Sbjct: 311 AITVDDFMSSFLENCVAYEQCHVACSMHFTTYATLLDCLTNTYKDVEYLCDQNI-IENYF 370

Query: 383 KKDEEVAVLFNGIMTSSSMGLSAAKELDEAINGVNKYYKGRPKVKASRVIKKYVYSSWRI 442
             D E+A   N +    +  ++    L +    VN+YYK    V+ +     Y  S W  
Sbjct: 371 GTDTELAKFVNSLGRDVAFDITQC-YLKDLFEEVNEYYKSSWHVEWATFKFTYFNSPWSF 430

Query: 443 LALMATLMVLGLLVLQSFCSVY 451
           ++ +A L++L L V+Q+  +V+
Sbjct: 431 VSALAALVLLVLSVIQTIYTVF 443

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7019868.14.5e-263100.00putative UPF0481 protein, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022923882.16.8e-25184.93putative UPF0481 protein At3g02645 [Cucurbita moschata][more]
XP_023520065.13.4e-25084.56putative UPF0481 protein At3g02645 [Cucurbita pepo subsp. pepo][more]
XP_023000998.14.5e-24783.27putative UPF0481 protein At3g02645 [Cucurbita maxima][more]
KAG6584271.13.9e-23895.18putative UPF0481 protein, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
P0C8972.3e-6033.72Putative UPF0481 protein At3g02645 OS=Arabidopsis thaliana OX=3702 GN=At3g02645 ... [more]
Match NameE-valueIdentityDescription
A0A6J1E7C33.3e-25184.93putative UPF0481 protein At3g02645 OS=Cucurbita moschata OX=3662 GN=LOC111431467... [more]
A0A6J1KP862.2e-24783.27putative UPF0481 protein At3g02645 OS=Cucurbita maxima OX=3661 GN=LOC111495266 P... [more]
A0A2R6QS226.3e-8541.12UPF0481 protein OS=Actinidia chinensis var. chinensis OX=1590841 GN=CEY00_Acc145... [more]
A0A7J0FLR84.2e-7338.69Transmembrane protein, putative OS=Actinidia rufa OX=165716 GN=Acr_13g0010710 PE... [more]
A0A7J7H0H18.8e-7135.67Uncharacterized protein OS=Camellia sinensis OX=4442 GN=HYC85_015366 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G02645.11.6e-6133.72Plant protein of unknown function (DUF247) [more]
AT3G50140.13.7e-2123.70Plant protein of unknown function (DUF247) [more]
AT3G50150.12.4e-2024.18Plant protein of unknown function (DUF247) [more]
AT3G50120.13.1e-2023.29Plant protein of unknown function (DUF247) [more]
AT2G36430.17.0e-2024.66Plant protein of unknown function (DUF247) [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004158Protein of unknown function DUF247, plantPFAMPF03140DUF247coord: 53..438
e-value: 1.8E-71
score: 241.5
IPR004158Protein of unknown function DUF247, plantPANTHERPTHR31549PROTEIN, PUTATIVE (DUF247)-RELATED-RELATEDcoord: 17..259
IPR004158Protein of unknown function DUF247, plantPANTHERPTHR31549PROTEIN, PUTATIVE (DUF247)-RELATED-RELATEDcoord: 299..448
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..21
NoneNo IPR availablePANTHERPTHR31549:SF177BNACNNG05850D PROTEINcoord: 299..448
NoneNo IPR availablePANTHERPTHR31549:SF177BNACNNG05850D PROTEINcoord: 17..259

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg07730-RACarg07730-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane