Carg07724 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg07724
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	RING-type domain-containing protein
Location	Carg_Chr13: 8645282 .. 8645959 (-)
RNA-Seq Expression	Carg07724
Synteny	Carg07724

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCCACCGTCGCCGCCGCCGCCGCCGTTTCCGGCCTCCACTGCGAGGTTTGGCAGCCCCAAGACTTCTCTGAAACTCACTGTTCTTTTTCTTCTTCTTCTTCTGAATCCCCTGAGTTCTTCATCGATTTCTATGTCTCCTCTTATGCAACTCAAGGCGACCAAATCATCAGCACTCTTCGTTACAAGAATTTCAGGCAAAGATGTGACGTTTTAACTCAAGATTCCTTCTCTTGGTCCGCAATTTCCACTATGCTATCCGAAACCAATGTGCCTTACCATCTTCAACCCTTTTTCATTCAGCAGATTTCCATTAGCGCCCGCGGAATTGCAACCGAACCCACTAACGCTCTCTCCCGCACCATTCCCATGGTCGTCGAGCTGGTGCTGCCTGAGGAGGCCATGGAGGATTCGGGTTATGGGTCGGACACCATTATGGGAACTGGGTCGCGCCGGGCGAGTAGAACTTCGATTCAGGAAATGGAGAGAGTTGAAATGAATGGGGTTTTGAGTGATTGTGTGATTTGTTTGGATGATATCGTTTCAGTTGGGTCTGAAATTGATGCACTTCGAATGCCTTGTTTACATATTTACCATCGAAATTGTATTCACAAATGGCTGCAACTCAGCAACCGCTGCCCCTTGTGTCGGTTTCAGATGCCATTGGAAGAGGAATGA

mRNA sequence

ATGTCCACCGTCGCCGCCGCCGCCGCCGTTTCCGGCCTCCACTGCGAGGTTTGGCAGCCCCAAGACTTCTCTGAAACTCACTGTTCTTTTTCTTCTTCTTCTTCTGAATCCCCTGAGTTCTTCATCGATTTCTATGTCTCCTCTTATGCAACTCAAGGCGACCAAATCATCAGCACTCTTCGTTACAAGAATTTCAGGCAAAGATGTGACGTTTTAACTCAAGATTCCTTCTCTTGGTCCGCAATTTCCACTATGCTATCCGAAACCAATGTGCCTTACCATCTTCAACCCTTTTTCATTCAGCAGATTTCCATTAGCGCCCGCGGAATTGCAACCGAACCCACTAACGCTCTCTCCCGCACCATTCCCATGGTCGTCGAGCTGGTGCTGCCTGAGGAGGCCATGGAGGATTCGGGTTATGGGTCGGACACCATTATGGGAACTGGGTCGCGCCGGGCGAGTAGAACTTCGATTCAGGAAATGGAGAGAGTTGAAATGAATGGGGTTTTGAGTGATTGTGTGATTTGTTTGGATGATATCGTTTCAGTTGGGTCTGAAATTGATGCACTTCGAATGCCTTGTTTACATATTTACCATCGAAATTGTATTCACAAATGGCTGCAACTCAGCAACCGCTGCCCCTTGTGTCGGTTTCAGATGCCATTGGAAGAGGAATGA

Coding sequence (CDS)

ATGTCCACCGTCGCCGCCGCCGCCGCCGTTTCCGGCCTCCACTGCGAGGTTTGGCAGCCCCAAGACTTCTCTGAAACTCACTGTTCTTTTTCTTCTTCTTCTTCTGAATCCCCTGAGTTCTTCATCGATTTCTATGTCTCCTCTTATGCAACTCAAGGCGACCAAATCATCAGCACTCTTCGTTACAAGAATTTCAGGCAAAGATGTGACGTTTTAACTCAAGATTCCTTCTCTTGGTCCGCAATTTCCACTATGCTATCCGAAACCAATGTGCCTTACCATCTTCAACCCTTTTTCATTCAGCAGATTTCCATTAGCGCCCGCGGAATTGCAACCGAACCCACTAACGCTCTCTCCCGCACCATTCCCATGGTCGTCGAGCTGGTGCTGCCTGAGGAGGCCATGGAGGATTCGGGTTATGGGTCGGACACCATTATGGGAACTGGGTCGCGCCGGGCGAGTAGAACTTCGATTCAGGAAATGGAGAGAGTTGAAATGAATGGGGTTTTGAGTGATTGTGTGATTTGTTTGGATGATATCGTTTCAGTTGGGTCTGAAATTGATGCACTTCGAATGCCTTGTTTACATATTTACCATCGAAATTGTATTCACAAATGGCTGCAACTCAGCAACCGCTGCCCCTTGTGTCGGTTTCAGATGCCATTGGAAGAGGAATGA

Protein sequence

MSTVAAAAAVSGLHCEVWQPQDFSETHCSFSSSSSESPEFFIDFYVSSYATQGDQIISTLRYKNFRQRCDVLTQDSFSWSAISTMLSETNVPYHLQPFFIQQISISARGIATEPTNALSRTIPMVVELVLPEEAMEDSGYGSDTIMGTGSRRASRTSIQEMERVEMNGVLSDCVICLDDIVSVGSEIDALRMPCLHIYHRNCIHKWLQLSNRCPLCRFQMPLEEE

Homology

[Close]

BLAST of Carg07724 vs. NCBI nr
Match: KAG7019862.1 (E3 ubiquitin-protein ligase SIRP1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 453.8 bits (1166), Expect = 8.9e-124
Identity = 225/225 (100.00%), Postives = 225/225 (100.00%), Query Frame = 0


Query: 1   MSTVAAAAAVSGLHCEVWQPQDFSETHCSFSSSSSESPEFFIDFYVSSYATQGDQIISTL 60
           MSTVAAAAAVSGLHCEVWQPQDFSETHCSFSSSSSESPEFFIDFYVSSYATQGDQIISTL
Sbjct: 1   MSTVAAAAAVSGLHCEVWQPQDFSETHCSFSSSSSESPEFFIDFYVSSYATQGDQIISTL 60

Query: 61  RYKNFRQRCDVLTQDSFSWSAISTMLSETNVPYHLQPFFIQQISISARGIATEPTNALSR 120
           RYKNFRQRCDVLTQDSFSWSAISTMLSETNVPYHLQPFFIQQISISARGIATEPTNALSR
Sbjct: 61  RYKNFRQRCDVLTQDSFSWSAISTMLSETNVPYHLQPFFIQQISISARGIATEPTNALSR 120

Query: 121 TIPMVVELVLPEEAMEDSGYGSDTIMGTGSRRASRTSIQEMERVEMNGVLSDCVICLDDI 180
           TIPMVVELVLPEEAMEDSGYGSDTIMGTGSRRASRTSIQEMERVEMNGVLSDCVICLDDI
Sbjct: 121 TIPMVVELVLPEEAMEDSGYGSDTIMGTGSRRASRTSIQEMERVEMNGVLSDCVICLDDI 180

Query: 181 VSVGSEIDALRMPCLHIYHRNCIHKWLQLSNRCPLCRFQMPLEEE 226
           VSVGSEIDALRMPCLHIYHRNCIHKWLQLSNRCPLCRFQMPLEEE
Sbjct: 181 VSVGSEIDALRMPCLHIYHRNCIHKWLQLSNRCPLCRFQMPLEEE 225

[Close]

BLAST of Carg07724 vs. NCBI nr
Match: KAG6584266.1 (E3 ubiquitin-protein ligase MPSR1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 450.3 bits (1157), Expect = 9.9e-123
Identity = 223/225 (99.11%), Postives = 223/225 (99.11%), Query Frame = 0


Query: 1   MSTVAAAAAVSGLHCEVWQPQDFSETHCSFSSSSSESPEFFIDFYVSSYATQGDQIISTL 60
           MST AAAAAVSGLHCEVWQPQDFSETHCSFSSSSSESPEFFIDFYVSSYATQGDQIISTL
Sbjct: 1   MSTAAAAAAVSGLHCEVWQPQDFSETHCSFSSSSSESPEFFIDFYVSSYATQGDQIISTL 60

Query: 61  RYKNFRQRCDVLTQDSFSWSAISTMLSETNVPYHLQPFFIQQISISARGIATEPTNALSR 120
           RYKNFRQRCDVLTQDSFSWSAISTMLSETNVPYHLQPFFIQQISISARGIATEPTNALSR
Sbjct: 61  RYKNFRQRCDVLTQDSFSWSAISTMLSETNVPYHLQPFFIQQISISARGIATEPTNALSR 120

Query: 121 TIPMVVELVLPEEAMEDSGYGSDTIMGTGSRRASRTSIQEMERVEMNGVLSDCVICLDDI 180
           TIPMVVELVLPEEAMEDSGYGSDTIMGTGSRRASR SIQEMERVEMNGVLSDCVICLDDI
Sbjct: 121 TIPMVVELVLPEEAMEDSGYGSDTIMGTGSRRASRASIQEMERVEMNGVLSDCVICLDDI 180

Query: 181 VSVGSEIDALRMPCLHIYHRNCIHKWLQLSNRCPLCRFQMPLEEE 226
           VSVGSEIDALRMPCLHIYHRNCIHKWLQLSNRCPLCRFQMPLEEE
Sbjct: 181 VSVGSEIDALRMPCLHIYHRNCIHKWLQLSNRCPLCRFQMPLEEE 225

[Close]

BLAST of Carg07724 vs. NCBI nr
Match: XP_023519803.1 (RING-H2 finger protein ATL77-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 447.2 bits (1149), Expect = 8.4e-122
Identity = 222/225 (98.67%), Postives = 222/225 (98.67%), Query Frame = 0


Query: 1   MSTVAAAAAVSGLHCEVWQPQDFSETHCSFSSSSSESPEFFIDFYVSSYATQGDQIISTL 60
           MSTVAAAAAVSGLHCEVWQPQD SETHCSFSSSSSE PEFFIDFYVSSYATQGDQIISTL
Sbjct: 1   MSTVAAAAAVSGLHCEVWQPQDLSETHCSFSSSSSEFPEFFIDFYVSSYATQGDQIISTL 60

Query: 61  RYKNFRQRCDVLTQDSFSWSAISTMLSETNVPYHLQPFFIQQISISARGIATEPTNALSR 120
           RYKNFRQRCDVLTQDSFSWSAISTMLSETNVPYHLQPFFIQQISISARGIATEPTNALSR
Sbjct: 61  RYKNFRQRCDVLTQDSFSWSAISTMLSETNVPYHLQPFFIQQISISARGIATEPTNALSR 120

Query: 121 TIPMVVELVLPEEAMEDSGYGSDTIMGTGSRRASRTSIQEMERVEMNGVLSDCVICLDDI 180
           TIPMVVELVLPEEAMEDSGYGSDTIMGTGSRRASR SIQEMERVEMNGVLSDCVICLDDI
Sbjct: 121 TIPMVVELVLPEEAMEDSGYGSDTIMGTGSRRASRASIQEMERVEMNGVLSDCVICLDDI 180

Query: 181 VSVGSEIDALRMPCLHIYHRNCIHKWLQLSNRCPLCRFQMPLEEE 226
           VSVGSEIDALRMPCLHIYHRNCIHKWLQLSNRCPLCRFQMPLEEE
Sbjct: 181 VSVGSEIDALRMPCLHIYHRNCIHKWLQLSNRCPLCRFQMPLEEE 225

[Close]

BLAST of Carg07724 vs. NCBI nr
Match: XP_023001523.1 (uncharacterized protein LOC111495638 [Cucurbita maxima])

HSP 1 Score: 446.4 bits (1147), Expect = 1.4e-121
Identity = 221/225 (98.22%), Postives = 221/225 (98.22%), Query Frame = 0


Query: 1   MSTVAAAAAVSGLHCEVWQPQDFSETHCSFSSSSSESPEFFIDFYVSSYATQGDQIISTL 60
           MST AAAAAVSGLHCEVWQPQDFSETHCSFSSSSSESPEFFIDFYVSSYATQGDQIISTL
Sbjct: 1   MSTAAAAAAVSGLHCEVWQPQDFSETHCSFSSSSSESPEFFIDFYVSSYATQGDQIISTL 60

Query: 61  RYKNFRQRCDVLTQDSFSWSAISTMLSETNVPYHLQPFFIQQISISARGIATEPTNALSR 120
           RYKNFRQRCDVLTQDSFSWSAISTMLSETNVPYHLQPFFIQQISISARGIATEPTNALSR
Sbjct: 61  RYKNFRQRCDVLTQDSFSWSAISTMLSETNVPYHLQPFFIQQISISARGIATEPTNALSR 120

Query: 121 TIPMVVELVLPEEAMEDSGYGSDTIMGTGSRRASRTSIQEMERVEMNGVLSDCVICLDDI 180
           TIPMVVELVLPEEAMEDS YGSDTIMGTGSRRASR SIQEMERVEMNG LSDCVICLDDI
Sbjct: 121 TIPMVVELVLPEEAMEDSSYGSDTIMGTGSRRASRASIQEMERVEMNGALSDCVICLDDI 180

Query: 181 VSVGSEIDALRMPCLHIYHRNCIHKWLQLSNRCPLCRFQMPLEEE 226
           VSVGSEIDALRMPCLHIYHRNCIHKWLQLSNRCPLCRFQMPLEEE
Sbjct: 181 VSVGSEIDALRMPCLHIYHRNCIHKWLQLSNRCPLCRFQMPLEEE 225

[Close]

BLAST of Carg07724 vs. NCBI nr
Match: XP_038895883.1 (E3 ubiquitin-protein ligase synoviolin A-like [Benincasa hispida])

HSP 1 Score: 382.5 bits (981), Expect = 2.5e-102
Identity = 191/222 (86.04%), Postives = 203/222 (91.44%), Query Frame = 0


Query: 5   AAAAAVSGLHCEVWQPQDFSETHCSFSSSS-SESPEFFIDFYVSSYATQGDQIISTLRYK 64
           AAA   SGLHCEVWQPQDFSET  S SSSS S+ PEFFIDFYVSSYATQGD+IISTLRYK
Sbjct: 4   AAANNNSGLHCEVWQPQDFSETPSSSSSSSQSQPPEFFIDFYVSSYATQGDEIISTLRYK 63

Query: 65  NFRQRCDVLTQDSFSWSAISTMLSETNVPYHLQPFFIQQISISARGIATEPTNALSRTIP 124
           NFRQRCDVLTQDSFSWSAIS+MLSETNVPYHLQPFFI QISI ARGIATEP NALSRTIP
Sbjct: 64  NFRQRCDVLTQDSFSWSAISSMLSETNVPYHLQPFFIHQISIRARGIATEPINALSRTIP 123

Query: 125 MVVELVLPEEAMEDSGYGSDTIMGTGSRRASRTSIQEMERVEMNGVLSDCVICLDDIVSV 184
           MVVEL+LPEEAMEDS YGSDT MG  S RASR SIQ+MERVE++GVLSDCVICLD+IVS+
Sbjct: 124 MVVELMLPEEAMEDSSYGSDTHMGIVSGRASRASIQDMERVEIDGVLSDCVICLDEIVSI 183

Query: 185 GSEIDALRMPCLHIYHRNCIHKWLQLSNRCPLCRFQMPLEEE 226
           G EIDA+RMPCLH+YHRNCIHKWL+ SNRCPLCRFQMPLEEE
Sbjct: 184 GYEIDAVRMPCLHVYHRNCIHKWLEFSNRCPLCRFQMPLEEE 225

[Close]

BLAST of Carg07724 vs. ExPASy Swiss-Prot
Match: Q9LQX2 (E3 ubiquitin-protein ligase MPSR1 OS=Arabidopsis thaliana OX=3702 GN=MPSR1 PE=1 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 1.4e-10
Identity = 32/75 (42.67%), Postives = 44/75 (58.67%), Query Frame = 0


Query: 149 GSRRASRTSIQEMERVEMNGVLSDCVICLDDIVSVGSEIDALRMPCLHIYHRNCIHKWLQ 208
           G   AS+ SI  M  VE++G   +CVICL++     SE     MPC H +H  CI KWL 
Sbjct: 89  GRPPASKASIDAMPIVEIDGCEGECVICLEE---WKSEETVKEMPCKHRFHGGCIEKWLG 148

Query: 209 LSNRCPLCRFQMPLE 224
               CP+CR++MP++
Sbjct: 149 FHGSCPVCRYEMPVD 160

[Close]

BLAST of Carg07724 vs. ExPASy Swiss-Prot
Match: Q6AVN2 (E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SIRP1 PE=1 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.8e-10
Identity = 32/76 (42.11%), Postives = 52/76 (68.42%), Query Frame = 0


Query: 149 GSRRASRTSIQEMERVEMNGVLSDCVICLDDIVSVGSEIDALRMPCLHIYHRNCIHKWLQ 208
           G+  A + +++ +  V++  V+S C +CLDD+  VGS+  A +MPC H +H +CI  WL+
Sbjct: 176 GTPPAKKEAVEALPTVKIEEVVS-CSVCLDDL-EVGSQ--AKQMPCEHKFHSSCILPWLE 235

Query: 209 LSNRCPLCRFQMPLEE 225
           L + CP+CRF++P EE
Sbjct: 236 LHSSCPVCRFELPSEE 247

[Close]

BLAST of Carg07724 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 5.3e-10
Identity = 29/81 (35.80%), Postives = 51/81 (62.96%), Query Frame = 0


Query: 149 GSRRASRTSIQEMERV-----EMNGVLSDCVICLDDIVSVGSEIDALRMPCLHIYHRNCI 208
           G+  AS+++I+ +  V      +N   + C +C+DD    G+E  A +MPC H+YH++C+
Sbjct: 195 GTPPASKSAIEALPLVNITKSNLNSEFNQCAVCMDDF-EEGTE--AKQMPCKHLYHKDCL 254

Query: 209 HKWLQLSNRCPLCRFQMPLEE 225
             WL+L N CP+CR ++P ++
Sbjct: 255 LPWLELHNSCPVCRHELPTDD 272

[Close]

BLAST of Carg07724 vs. ExPASy Swiss-Prot
Match: Q940T5 (E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 6.9e-10
Identity = 29/80 (36.25%), Postives = 51/80 (63.75%), Query Frame = 0


Query: 148 TGSRRASRTSIQEMERVEMN----GVLSDCVICLDDIVSVGSEIDALRMPCLHIYHRNCI 207
           +G+  AS+++I+ + RVE++    G  ++C +C +      +E +A  MPC H++H +CI
Sbjct: 170 SGNPPASKSAIESLPRVEISDCHIGSEANCAVCTE---IFETETEAREMPCKHLFHDDCI 229

Query: 208 HKWLQLSNRCPLCRFQMPLE 224
             WL + N CP+CRF++P E
Sbjct: 230 VPWLSIRNSCPVCRFELPSE 246

[Close]

BLAST of Carg07724 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 2.0e-09
Identity = 27/81 (33.33%), Postives = 51/81 (62.96%), Query Frame = 0


Query: 149 GSRRASRTSIQEMERVE-----MNGVLSDCVICLDDIVSVGSEIDALRMPCLHIYHRNCI 208
           G+  AS+++I  +  V+     +   ++ C +C+D+    GS  D  +MPC H++H++C+
Sbjct: 187 GTPPASKSAIDALPTVKVTKDMLKSEMNQCAVCMDEFED-GS--DVKQMPCKHVFHQDCL 246

Query: 209 HKWLQLSNRCPLCRFQMPLEE 225
             WL+L N CP+CRF++P ++
Sbjct: 247 LPWLELHNSCPVCRFELPTDD 264

[Close]

BLAST of Carg07724 vs. ExPASy TrEMBL
Match: A0A6J1KQS3 (uncharacterized protein LOC111495638 OS=Cucurbita maxima OX=3661 GN=LOC111495638 PE=4 SV=1)

HSP 1 Score: 446.4 bits (1147), Expect = 6.9e-122
Identity = 221/225 (98.22%), Postives = 221/225 (98.22%), Query Frame = 0


Query: 1   MSTVAAAAAVSGLHCEVWQPQDFSETHCSFSSSSSESPEFFIDFYVSSYATQGDQIISTL 60
           MST AAAAAVSGLHCEVWQPQDFSETHCSFSSSSSESPEFFIDFYVSSYATQGDQIISTL
Sbjct: 1   MSTAAAAAAVSGLHCEVWQPQDFSETHCSFSSSSSESPEFFIDFYVSSYATQGDQIISTL 60

Query: 61  RYKNFRQRCDVLTQDSFSWSAISTMLSETNVPYHLQPFFIQQISISARGIATEPTNALSR 120
           RYKNFRQRCDVLTQDSFSWSAISTMLSETNVPYHLQPFFIQQISISARGIATEPTNALSR
Sbjct: 61  RYKNFRQRCDVLTQDSFSWSAISTMLSETNVPYHLQPFFIQQISISARGIATEPTNALSR 120

Query: 121 TIPMVVELVLPEEAMEDSGYGSDTIMGTGSRRASRTSIQEMERVEMNGVLSDCVICLDDI 180
           TIPMVVELVLPEEAMEDS YGSDTIMGTGSRRASR SIQEMERVEMNG LSDCVICLDDI
Sbjct: 121 TIPMVVELVLPEEAMEDSSYGSDTIMGTGSRRASRASIQEMERVEMNGALSDCVICLDDI 180

Query: 181 VSVGSEIDALRMPCLHIYHRNCIHKWLQLSNRCPLCRFQMPLEEE 226
           VSVGSEIDALRMPCLHIYHRNCIHKWLQLSNRCPLCRFQMPLEEE
Sbjct: 181 VSVGSEIDALRMPCLHIYHRNCIHKWLQLSNRCPLCRFQMPLEEE 225

[Close]

BLAST of Carg07724 vs. ExPASy TrEMBL
Match: A0A5D3BJ05 (E3 ubiquitin-protein ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G003020 PE=4 SV=1)

HSP 1 Score: 382.1 bits (980), Expect = 1.6e-102
Identity = 186/223 (83.41%), Postives = 204/223 (91.48%), Query Frame = 0


Query: 4   VAAAAAVSGLHCEVWQPQDFSETHCSFSSSSS-ESPEFFIDFYVSSYATQGDQIISTLRY 63
           ++AAAA+SGLHCEVWQPQDFSE  CS SSSS  + PEFFIDFYVSSYAT GD++ISTLRY
Sbjct: 1   MSAAAAISGLHCEVWQPQDFSEPSCSSSSSSQFQPPEFFIDFYVSSYATHGDEVISTLRY 60

Query: 64  KNFRQRCDVLTQDSFSWSAISTMLSETNVPYHLQPFFIQQISISARGIATEPTNALSRTI 123
           KNFRQRCDVLTQDSFSWSAIS+MLSETNVPYHLQPFFI QISI ARGIATEP NALSRTI
Sbjct: 61  KNFRQRCDVLTQDSFSWSAISSMLSETNVPYHLQPFFIHQISIRARGIATEPINALSRTI 120

Query: 124 PMVVELVLPEEAMEDSGYGSDTIMGTGSRRASRTSIQEMERVEMNGVLSDCVICLDDIVS 183
           PMVVEL+LPE+AME SGYGS+  MG GS RASR SIQEMER+E++GVLSDCVICLD+I S
Sbjct: 121 PMVVELMLPEDAMEGSGYGSEPHMGIGSGRASRASIQEMERIEIDGVLSDCVICLDEIGS 180

Query: 184 VGSEIDALRMPCLHIYHRNCIHKWLQLSNRCPLCRFQMPLEEE 226
           +G EIDA+RMPCLH+YH NCIHKWL+LSNRCPLCRFQMPLEEE
Sbjct: 181 IGCEIDAVRMPCLHVYHLNCIHKWLELSNRCPLCRFQMPLEEE 223

[Close]

BLAST of Carg07724 vs. ExPASy TrEMBL
Match: A0A1S3AU71 (uncharacterized protein LOC103482872 OS=Cucumis melo OX=3656 GN=LOC103482872 PE=4 SV=1)

HSP 1 Score: 382.1 bits (980), Expect = 1.6e-102
Identity = 186/223 (83.41%), Postives = 204/223 (91.48%), Query Frame = 0


Query: 4   VAAAAAVSGLHCEVWQPQDFSETHCSFSSSSS-ESPEFFIDFYVSSYATQGDQIISTLRY 63
           ++AAAA+SGLHCEVWQPQDFSE  CS SSSS  + PEFFIDFYVSSYAT GD++ISTLRY
Sbjct: 1   MSAAAAISGLHCEVWQPQDFSEPSCSSSSSSQFQPPEFFIDFYVSSYATHGDEVISTLRY 60

Query: 64  KNFRQRCDVLTQDSFSWSAISTMLSETNVPYHLQPFFIQQISISARGIATEPTNALSRTI 123
           KNFRQRCDVLTQDSFSWSAIS+MLSETNVPYHLQPFFI QISI ARGIATEP NALSRTI
Sbjct: 61  KNFRQRCDVLTQDSFSWSAISSMLSETNVPYHLQPFFIHQISIRARGIATEPINALSRTI 120

Query: 124 PMVVELVLPEEAMEDSGYGSDTIMGTGSRRASRTSIQEMERVEMNGVLSDCVICLDDIVS 183
           PMVVEL+LPE+AME SGYGS+  MG GS RASR SIQEMER+E++GVLSDCVICLD+I S
Sbjct: 121 PMVVELMLPEDAMEGSGYGSEPHMGIGSGRASRASIQEMERIEIDGVLSDCVICLDEIGS 180

Query: 184 VGSEIDALRMPCLHIYHRNCIHKWLQLSNRCPLCRFQMPLEEE 226
           +G EIDA+RMPCLH+YH NCIHKWL+LSNRCPLCRFQMPLEEE
Sbjct: 181 IGCEIDAVRMPCLHVYHLNCIHKWLELSNRCPLCRFQMPLEEE 223

[Close]

BLAST of Carg07724 vs. ExPASy TrEMBL
Match: A0A0A0LR28 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G045460 PE=4 SV=1)

HSP 1 Score: 377.1 bits (967), Expect = 5.1e-101
Identity = 182/219 (83.11%), Postives = 199/219 (90.87%), Query Frame = 0


Query: 9   AVSGLHCEVWQPQDFSETHCSFSSSSS--ESPEFFIDFYVSSYATQGDQIISTLRYKNFR 68
           A+SGLHCEVWQPQDFSE  CS SSSSS  + PEFFIDFYVSSYAT GD++ISTLRYKNFR
Sbjct: 5   AISGLHCEVWQPQDFSEPSCSSSSSSSQFQPPEFFIDFYVSSYATHGDEVISTLRYKNFR 64

Query: 69  QRCDVLTQDSFSWSAISTMLSETNVPYHLQPFFIQQISISARGIATEPTNALSRTIPMVV 128
           QRCDVLTQDSFSWSAIS+MLSETNVPYHLQPFFI QIS  ARGIATEP NALSRTIPMVV
Sbjct: 65  QRCDVLTQDSFSWSAISSMLSETNVPYHLQPFFIHQISTRARGIATEPINALSRTIPMVV 124

Query: 129 ELVLPEEAMEDSGYGSDTIMGTGSRRASRTSIQEMERVEMNGVLSDCVICLDDIVSVGSE 188
           EL+LPE+AMEDSGYGS+  MG GS RASR SIQEMER+E++GVLSDCVICLD+I S+G E
Sbjct: 125 ELILPEDAMEDSGYGSEPHMGIGSGRASRASIQEMERIEIDGVLSDCVICLDEIGSIGCE 184

Query: 189 IDALRMPCLHIYHRNCIHKWLQLSNRCPLCRFQMPLEEE 226
           ID ++MPCLH+YH NCIHKWL+LSNRCPLCRFQMPLEEE
Sbjct: 185 IDVVQMPCLHVYHLNCIHKWLELSNRCPLCRFQMPLEEE 223

[Close]

BLAST of Carg07724 vs. ExPASy TrEMBL
Match: A0A6J1CAF5 (E3 ubiquitin-protein ligase CIP8-like OS=Momordica charantia OX=3673 GN=LOC111008905 PE=4 SV=1)

HSP 1 Score: 287.3 bits (734), Expect = 5.4e-74
Identity = 150/219 (68.49%), Postives = 171/219 (78.08%), Query Frame = 0


Query: 9   AVSGLHCEVWQPQDFSETHCSFSSSSSESPEFFIDFYVSSYATQGDQIISTLRYKNFRQR 68
           + +GLHCEVWQPQ+++E+ C  SSSSSE PEFFID YVSSYA QG + IS+LRYKNFR+R
Sbjct: 2   SAAGLHCEVWQPQEWAESDC--SSSSSEPPEFFIDLYVSSYANQGGETISSLRYKNFRRR 61

Query: 69  CDVLTQDSFSWSAISTMLSETNVPYHLQPFFIQQISISARGIATEPTNALSRTIPMVVEL 128
            DV TQDS SWSAISTMLSET+VPYHLQPFFIQ IS+ AR IA +PTNALSRTIP+VVEL
Sbjct: 62  RDVFTQDSLSWSAISTMLSETSVPYHLQPFFIQHISLRARRIAAQPTNALSRTIPIVVEL 121

Query: 129 VLPEEAMEDSGYGSDTIMGTGSRRASRTSIQEMERVEMN-GVL-SDCVICLDDIVSVGSE 188
           VLP    E+SG GSD  +  G  RASR SI+EMER E+  G+   DC ICLD+I     E
Sbjct: 122 VLPLPEEEESGSGSDPRVPNGPGRASRASIEEMERAEIELGICGGDCAICLDEI-----E 181

Query: 189 IDALRMPCLHIYHRNCIHKWLQLSNRCPLCRFQMPLEEE 226
             A+ MPC HIYHRNCI KWLQ +N CPLCRFQMPLE E
Sbjct: 182 CGAVGMPCSHIYHRNCIVKWLQQNNLCPLCRFQMPLEME 213

[Close]

BLAST of Carg07724 vs. TAIR 10
Match: AT1G26800.1 (RING/U-box superfamily protein )

HSP 1 Score: 68.2 bits (165), Expect = 9.8e-12
Identity = 32/75 (42.67%), Postives = 44/75 (58.67%), Query Frame = 0


Query: 149 GSRRASRTSIQEMERVEMNGVLSDCVICLDDIVSVGSEIDALRMPCLHIYHRNCIHKWLQ 208
           G   AS+ SI  M  VE++G   +CVICL++     SE     MPC H +H  CI KWL 
Sbjct: 89  GRPPASKASIDAMPIVEIDGCEGECVICLEE---WKSEETVKEMPCKHRFHGGCIEKWLG 148

Query: 209 LSNRCPLCRFQMPLE 224
               CP+CR++MP++
Sbjct: 149 FHGSCPVCRYEMPVD 160

[Close]

BLAST of Carg07724 vs. TAIR 10
Match: AT5G59550.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 65.9 bits (159), Expect = 4.9e-11
Identity = 29/80 (36.25%), Postives = 51/80 (63.75%), Query Frame = 0


Query: 148 TGSRRASRTSIQEMERVEMN----GVLSDCVICLDDIVSVGSEIDALRMPCLHIYHRNCI 207
           +G+  AS+++I+ + RVE++    G  ++C +C +      +E +A  MPC H++H +CI
Sbjct: 170 SGNPPASKSAIESLPRVEISDCHIGSEANCAVCTE---IFETETEAREMPCKHLFHDDCI 229

Query: 208 HKWLQLSNRCPLCRFQMPLE 224
             WL + N CP+CRF++P E
Sbjct: 230 VPWLSIRNSCPVCRFELPSE 246

[Close]

BLAST of Carg07724 vs. TAIR 10
Match: AT1G60360.1 (RING/U-box superfamily protein )

HSP 1 Score: 64.3 bits (155), Expect = 1.4e-10
Identity = 29/79 (36.71%), Postives = 46/79 (58.23%), Query Frame = 0


Query: 149 GSRRASRTSIQEMERVE-----MNGVLSDCVICLDDIVSVGSEIDALRMPCLHIYHRNCI 208
           G   AS  +I  +  V+     +   +S C +C+++ +  G   DA  +PC HIYH++CI
Sbjct: 195 GPPPASEPTINSLPSVKITPQHLTNDMSQCTVCMEEFIVGG---DATELPCKHIYHKDCI 254

Query: 209 HKWLQLSNRCPLCRFQMPL 223
             WL+L+N CP+CR  +PL
Sbjct: 255 VPWLRLNNSCPICRRDLPL 270

[Close]

BLAST of Carg07724 vs. TAIR 10
Match: AT3G19950.1 (RING/U-box superfamily protein )

HSP 1 Score: 64.3 bits (155), Expect = 1.4e-10
Identity = 27/81 (33.33%), Postives = 51/81 (62.96%), Query Frame = 0


Query: 149 GSRRASRTSIQEMERVE-----MNGVLSDCVICLDDIVSVGSEIDALRMPCLHIYHRNCI 208
           G+  AS+++I  +  V+     +   ++ C +C+D+    GS  D  +MPC H++H++C+
Sbjct: 187 GTPPASKSAIDALPTVKVTKDMLKSEMNQCAVCMDEFED-GS--DVKQMPCKHVFHQDCL 246

Query: 209 HKWLQLSNRCPLCRFQMPLEE 225
             WL+L N CP+CRF++P ++
Sbjct: 247 LPWLELHNSCPVCRFELPTDD 264

[Close]

BLAST of Carg07724 vs. TAIR 10
Match: AT3G46620.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 63.2 bits (152), Expect = 3.2e-10
Identity = 33/91 (36.26%), Postives = 55/91 (60.44%), Query Frame = 0


Query: 135 MEDSGYGSDTIMGTGSRRASRTSIQEMERVEMNG----VLSDCVICLDDIVSVGSEIDAL 194
           +E SG G   I  +G+  AS+++I+ + RVE++       ++C +C  ++   G  I+  
Sbjct: 176 IEASGNG---IGRSGNPPASKSAIESLPRVEISDCHTKAEANCAVC-TEVFEAG--IEGR 235

Query: 195 RMPCLHIYHRNCIHKWLQLSNRCPLCRFQMP 222
            MPC HI+H +CI  WL + N CP+CRF++P
Sbjct: 236 EMPCKHIFHGDCIVPWLSIRNSCPVCRFELP 260

The following BLAST results are available for this feature:

BLAST of Carg07724 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7019862.1	8.9e-124	100.00	E3 ubiquitin-protein ligase SIRP1, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KAG6584266.1	9.9e-123	99.11	E3 ubiquitin-protein ligase MPSR1, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023519803.1	8.4e-122	98.67	RING-H2 finger protein ATL77-like [Cucurbita pepo subsp. pepo]	[more]
XP_023001523.1	1.4e-121	98.22	uncharacterized protein LOC111495638 [Cucurbita maxima]	[more]
XP_038895883.1	2.5e-102	86.04	E3 ubiquitin-protein ligase synoviolin A-like [Benincasa hispida]	[more]

BLAST of Carg07724 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LQX2	1.4e-10	42.67	E3 ubiquitin-protein ligase MPSR1 OS=Arabidopsis thaliana OX=3702 GN=MPSR1 PE=1 ...	[more]
Q6AVN2	1.8e-10	42.11	E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SI...	[more]
P0CH30	5.3e-10	35.80	E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV...	[more]
Q940T5	6.9e-10	36.25	E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 ...	[more]
Q8LPN7	2.0e-09	33.33	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19...	[more]

BLAST of Carg07724 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KQS3	6.9e-122	98.22	uncharacterized protein LOC111495638 OS=Cucurbita maxima OX=3661 GN=LOC111495638...	[more]
A0A5D3BJ05	1.6e-102	83.41	E3 ubiquitin-protein ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]
A0A1S3AU71	1.6e-102	83.41	uncharacterized protein LOC103482872 OS=Cucumis melo OX=3656 GN=LOC103482872 PE=...	[more]
A0A0A0LR28	5.1e-101	83.11	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G045460 P...	[more]
A0A6J1CAF5	5.4e-74	68.49	E3 ubiquitin-protein ligase CIP8-like OS=Momordica charantia OX=3673 GN=LOC11100...	[more]

BLAST of Carg07724 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G26800.1	9.8e-12	42.67	RING/U-box superfamily protein	[more]
AT5G59550.1	4.9e-11	36.25	zinc finger (C3HC4-type RING finger) family protein	[more]
AT1G60360.1	1.4e-10	36.71	RING/U-box superfamily protein	[more]
AT3G19950.1	1.4e-10	33.33	RING/U-box superfamily protein	[more]
AT3G46620.1	3.2e-10	36.26	zinc finger (C3HC4-type RING finger) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 173..216 e-value: 2.2E-7 score: 40.5
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 172..217 e-value: 7.4E-12 score: 45.4
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 173..217 score: 12.415513
IPR011016	Zinc finger, RING-CH-type	SMART	SM00744	ringv_2	coord: 172..217 e-value: 0.003 score: 11.7
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 142..222 e-value: 3.8E-17 score: 63.7
None	No IPR available	PANTHER	PTHR15710:SF138	FINGER PROTEIN, PUTATIVE-RELATED	coord: 14..221
None	No IPR available	PANTHER	PTHR15710	E3 UBIQUITIN-PROTEIN LIGASE PRAJA	coord: 14..221
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 172..221

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg07724-RA	Carg07724-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0061630	ubiquitin protein ligase activity
molecular_function	GO:0008270	zinc ion binding