Carg07677 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg07677
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionIron-sulfur cluster assembly protein
LocationCarg_Chr13: 8412350 .. 8415455 (+)
RNA-Seq ExpressionCarg07677
SyntenyCarg07677
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGATAAGAAAAAGCAATAAAGAATCAGACTCCGGAACTCCAGGGGAGAACGAAAGCGGTGAATATGTTGAGGTTCAGTTCGAAGAGGCTTCTAGGGATTGCGTCGGGGAATATGGCGGCACCGGCGGTTCAAGTCGTCCCGCGTTTCTATCATGAGAGGGTTATTGATCACTACAACAACCCTCGAAATGTTGGATCGTTTGATAAGAACGATCCGACGGTCGGCACTGGCCTCGTTGGTGCGCCTGCCTGTGGCGATGTTATGAAGCTTCAGATCAAGGTTGATGAGAAGACTGGTAAGGTTGTTGATGCTTGCTTCAAGACGTTCGGATGCGGCTCTGCGATTGCTTCCTCGTCGTTGGGTTCGTTTTCTTCTTTTTATCCTTGATTTTTGTTCCTGGGAGATGTTGAGCGCAATTCTGATATTTGAATCCTTCGAGAACGCGTCATTTCTTTTTTATTAGTCCAGGATTTTGTGAATTAATGTATTTCTTGAGTTTTTTGAATCGTCGTTGTTATGCTGGATTTGTTTTATTTTCTCTTTAGGTATTTGTGATATTTTATCGCTGTACTATTCTTAAGGATAAAGGGGTGGGCTTTTAAACGTCGTACCTTTCTTTTATATCGTTGTTGCGGACTTGATATGTGGGCTTCAATCGGAACCGTTGGGATAAATTGCTTACAGTTACCATGCGTTATTCCATTCGAGTTTCACGATTTAGAAATCTTATTAGTGACCGGCTTGAGAATCTGTGTAGCCTGGTTTTTGTATAAATGGGTGCTGAGTTTCTTTGAATGGGACAAAAATACTATTGAAGTTTTCATGAGAAACGTCACCATATGGATTAATCTCCATGGCCTTTGGAAATCTTGTCATGATCATCTAATTTGGAAATCCTGTCAATCTTCATGGCCTTGTTCTCCCAAGGTCGGTGGGAGTGCCACTCTCTCTTTCTTTACCGTTCGGAATATGCAAAATTAAAATCTTGGTCTCTTTGTGAGCCTTCACTTGGAGTAAATAAGGTTTTGAGTTTTATTTTTTTATTTTTTTTACATATGGGATAAAGTGGTTTATCTCTCTCCGCATTCTGCCTGTGTATTTGTTCTTTACAAGTTTTAAGTACTTATATGGCGTTCAGTAAATTGTATCTACGTCCTCCTAAATGAGTTTTGAGGTGTTAGGCCCTTTTTCTTTTTGAATTGGAGGTGAATCGTGAATCTTTTGGTCCTGATCAGGTCATATGTGTATATCTTCATTGATATGTGGATTTTGAACTTGCAGCTACAGAAATGGTGAAAGGGAAGCAAATGGAAGAAGCCTTAACCATTAAAAATTCGTAAGTTAACATTCATTTTGAACCTTTTCATCTCATTCTTGTTCAACATGTTTTCCATACATTATTCATTTGATTTGGGTTATTTGTGACCATCTTAAAATTTGATGTCTATCTACAGCTTACGCCATCTAAATTTGAATTTTTGTTTCTATTGATTGAACAACTAACATGTAACAACTCTAGTTTTCTTGTGCACTGAATGACGTGTACTTCAGTTGGTACTTAGCTTCGGTGAAGGATACCATGTTTTCTTACTTGCATGTTAATGCAGGCAGTCATGTCATAAGTTAGCAGGTTTAGGATCACGAACTAAAACAAGCTTGCAGTTTAAATGCCCTGAAGAACCTTTGAAAAAGTTGTTAATGATGTAAAAATAATGGTATCAACTTGGTTTTCCTCCCTCTCATTCTTTCAGTGTACAATTCTCCTCTTGGATATTTTTGAATTTCGAGTATCCAATTTGATGATTGCATTATTGCTAACAAGCCCTTTTGGCTTTGAGGTTTTAACTTGTGAAATAACGTGGGTGTATACCAATTCTGCAGGGAAATCGCAAAGCATCTCTCCCTACCACCTGTTAAGTTGCACTGCAGCATGCTTGCTGAAGATGCTATCAAGGCTGCTGTTAAAGATTATGAAGCGAAGCGTGCTAAATTGGTGGATGGTGCTGACTCTCCTCCAGTGGTAAAGGCTTCAAGTGCTTAGCTAACTAATGATTGTAAATGATGTAAAAACTGTTATAGTCAAAATCTCCTGTCCAAGGTGCGAGTTCTCTTTCCAGTTTTTTTATATAGGATGTCAATCATAATTATCACCATATTTTACTTTAAATTGTGTTTCACCGATTTCCCCTTCCTGGGTGAAGTGGGTGAAAGTGTTGGACCGTTGGATTCTTGGTTCTTCAATCCCACGTTTGAAAGTCCAATGATGTTGCCGTGCCTATTTCACCGATTGTATGTACATTAACAGCTTACTGATTGAGATTGCAGTTTCATTTCTATCTTTATAAAGCCGACTTTGGTATATGTTATATAAAACCCTGTAAAAAGTGGGAGAATAGATGTGACTTTTGATTGGGTAAGTAATGTTTTGTTAATTAGATTTATAGTCTCTTTGGGATGATTTTTCAACTTTTTTTTGTAGATTCTTTTTTATGCTCGCAGCAGGGCATTTGAATTAGGTGTTGACTGTTCTGTTTTTTTTAACAATTATGTTCTACATTGTGTATCTTTGTGTGCGAGTGTAGCCATATAAACAGGTTAGATGTTACAGTGCTTGGAGGGACTGATTATGATCATTATGATCATATGGCTGATTGGCTATTAGTTTTTGAAGCCTGATTCGCTAACAGAGCTTCTAATGTATGATCATCACTGACATTCCGTACTTCTTGAAAATGGATTTATAATATAGTTAGGGGCAGGCAGGGCATTCTGTCTCTTACAATTGTTTCAAAGTTATGCTTTTGATCTCGGTAGCAAAGCAGCTTTACCGACAAGTTAAACGAGGATGCAAATTATATAGCAGCAGGGGTTGGGGAGTTATTGGTGAAACGTTAGGAGGTTCTCTATGCGTGGCAGCTCCCTGCCATCGAACAACAAGTTTTGCCTCTTCGTCTTGAAAGTACGTATATTATGTCGGTGTGAGACTTTAGAACTTTGTACCTTTCTTGGTTTGGTTTGGATAGAATAGAAGTCGCCTAACGTGCATTTTGGCAACAACTTTGTGTTCATCAAACATAACCACTTAATTTGCAGGAAGCTACACTAC

mRNA sequence

AGATAAGAAAAAGCAATAAAGAATCAGACTCCGGAACTCCAGGGGAGAACGAAAGCGGTGAATATGTTGAGGTTCAGTTCGAAGAGGCTTCTAGGGATTGCGTCGGGGAATATGGCGGCACCGGCGGTTCAAGTCGTCCCGCGTTTCTATCATGAGAGGGTTATTGATCACTACAACAACCCTCGAAATGTTGGATCGTTTGATAAGAACGATCCGACGGTCGGCACTGGCCTCGTTGGTGCGCCTGCCTGTGGCGATGTTATGAAGCTTCAGATCAAGGTTGATGAGAAGACTGGTAAGGTTGTTGATGCTTGCTTCAAGACGTTCGGATGCGGCTCTGCGATTGCTTCCTCGTCGTTGGCTACAGAAATGGTGAAAGGGAAGCAAATGGAAGAAGCCTTAACCATTAAAAATTCGGAAATCGCAAAGCATCTCTCCCTACCACCTGTTAAGTTGCACTGCAGCATGCTTGCTGAAGATGCTATCAAGGCTGCTGTTAAAGATTATGAAGCGAAGCGTGCTAAATTGGTGGATGGTGCTGACTCTCCTCCAGTGGTAAAGGCTTCAAGTGCTTAGCTAACTAATGATTGTAAATGATGTAAAAACTGTTATAGTCAAAATCTCCTGTCCAAGGTGCGAGTTCTCTTTCCAGTTTTTTTATATAGGATGTCAATCATAATTATCACCATATTTTACTTTAAATTGTGTTTCACCGATTTCCCCTTCCTGGGTGAAGTGGGTGAAAGTGTTGGACCGTTGGATTCTTGGTTCTTCAATCCCACGTTTGAAAGTCCAATGATGTTGCCGTGCCTATTTCACCGATTGTATGTACATTAACAGCTTACTGATTGAGATTGCAGTTTCATTTCTATCTTTATAAAGCCGACTTTGGTATATGTTATATAAAACCCTGTAAAAAGTGGGAGAATAGATGTGACTTTTGATTGGGTAAGTAATGTTTTGTTAATTAGATTTATAGTCTCTTTGGGATGATTTTTCAACTTTTTTTTGTAGATTCTTTTTTATGCTCGCAGCAGGGCATTTGAATTAGGTGTTGACTGTTCTGTTTTTTTTAACAATTATGTTCTACATTGTGTATCTTTGTGTGCGAGTGTAGCCATATAAACAGGTTAGATGTTACAGTGCTTGGAGGGACTGATTATGATCATTATGATCATATGGCTGATTGGCTATTAGTTTTTGAAGCCTGATTCGCTAACAGAGCTTCTAATGTATGATCATCACTGACATTCCGTACTTCTTGAAAATGGATTTATAATATAGTTAGGGGCAGGCAGGGCATTCTGTCTCTTACAATTGTTTCAAAGTTATGCTTTTGATCTCGGTAGCAAAGCAGCTTTACCGACAAGTTAAACGAGGATGCAAATTATATAGCAGCAGGGGTTGGGGAGTTATTGGTGAAACGTTAGGAGGTTCTCTATGCGTGGCAGCTCCCTGCCATCGAACAACAAGTTTTGCCTCTTCGTCTTGAAAGTACGTATATTATGTCGGTGTGAGACTTTAGAACTTTGTACCTTTCTTGGTTTGGTTTGGATAGAATAGAAGTCGCCTAACGTGCATTTTGGCAACAACTTTGTGTTCATCAAACATAACCACTTAATTTGCAGGAAGCTACACTAC

Coding sequence (CDS)

ATGTTGAGGTTCAGTTCGAAGAGGCTTCTAGGGATTGCGTCGGGGAATATGGCGGCACCGGCGGTTCAAGTCGTCCCGCGTTTCTATCATGAGAGGGTTATTGATCACTACAACAACCCTCGAAATGTTGGATCGTTTGATAAGAACGATCCGACGGTCGGCACTGGCCTCGTTGGTGCGCCTGCCTGTGGCGATGTTATGAAGCTTCAGATCAAGGTTGATGAGAAGACTGGTAAGGTTGTTGATGCTTGCTTCAAGACGTTCGGATGCGGCTCTGCGATTGCTTCCTCGTCGTTGGCTACAGAAATGGTGAAAGGGAAGCAAATGGAAGAAGCCTTAACCATTAAAAATTCGGAAATCGCAAAGCATCTCTCCCTACCACCTGTTAAGTTGCACTGCAGCATGCTTGCTGAAGATGCTATCAAGGCTGCTGTTAAAGATTATGAAGCGAAGCGTGCTAAATTGGTGGATGGTGCTGACTCTCCTCCAGTGGTAAAGGCTTCAAGTGCTTAG

Protein sequence

MLRFSSKRLLGIASGNMAAPAVQVVPRFYHERVIDHYNNPRNVGSFDKNDPTVGTGLVGAPACGDVMKLQIKVDEKTGKVVDACFKTFGCGSAIASSSLATEMVKGKQMEEALTIKNSEIAKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAKLVDGADSPPVVKASSA
Homology
BLAST of Carg07677 vs. NCBI nr
Match: KAG6584219.1 (Iron-sulfur cluster assembly protein 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019817.1 Iron-sulfur cluster assembly protein 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 334.3 bits (856), Expect = 6.0e-88
Identity = 170/170 (100.00%), Postives = 170/170 (100.00%), Query Frame = 0

Query: 1   MLRFSSKRLLGIASGNMAAPAVQVVPRFYHERVIDHYNNPRNVGSFDKNDPTVGTGLVGA 60
           MLRFSSKRLLGIASGNMAAPAVQVVPRFYHERVIDHYNNPRNVGSFDKNDPTVGTGLVGA
Sbjct: 1   MLRFSSKRLLGIASGNMAAPAVQVVPRFYHERVIDHYNNPRNVGSFDKNDPTVGTGLVGA 60

Query: 61  PACGDVMKLQIKVDEKTGKVVDACFKTFGCGSAIASSSLATEMVKGKQMEEALTIKNSEI 120
           PACGDVMKLQIKVDEKTGKVVDACFKTFGCGSAIASSSLATEMVKGKQMEEALTIKNSEI
Sbjct: 61  PACGDVMKLQIKVDEKTGKVVDACFKTFGCGSAIASSSLATEMVKGKQMEEALTIKNSEI 120

Query: 121 AKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAKLVDGADSPPVVKASSA 171
           AKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAKLVDGADSPPVVKASSA
Sbjct: 121 AKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAKLVDGADSPPVVKASSA 170

BLAST of Carg07677 vs. NCBI nr
Match: XP_038895391.1 (iron-sulfur cluster assembly protein 1-like [Benincasa hispida])

HSP 1 Score: 312.0 bits (798), Expect = 3.2e-81
Identity = 158/170 (92.94%), Postives = 165/170 (97.06%), Query Frame = 0

Query: 1   MLRFSSKRLLGIASGNMAAPAVQVVPRFYHERVIDHYNNPRNVGSFDKNDPTVGTGLVGA 60
           MLRFSSKRLLGIAS ++ +PAVQ+VPRFYHERVIDHYNNPRNVGSFDKNDPTVGTGLVGA
Sbjct: 1   MLRFSSKRLLGIASRDLPSPAVQIVPRFYHERVIDHYNNPRNVGSFDKNDPTVGTGLVGA 60

Query: 61  PACGDVMKLQIKVDEKTGKVVDACFKTFGCGSAIASSSLATEMVKGKQMEEALTIKNSEI 120
           PACGDVMKLQIKVDEKTGKVVDACFKTFGCGSAIASSSLATEMVKGKQMEEALTIKNSEI
Sbjct: 61  PACGDVMKLQIKVDEKTGKVVDACFKTFGCGSAIASSSLATEMVKGKQMEEALTIKNSEI 120

Query: 121 AKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAKLVDGADSPPVVKASSA 171
           AKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAKL +GA+S PV KAS+A
Sbjct: 121 AKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAKLANGAESLPVDKASNA 170

BLAST of Carg07677 vs. NCBI nr
Match: XP_022137708.1 (uncharacterized protein LOC111009078 [Momordica charantia])

HSP 1 Score: 310.8 bits (795), Expect = 7.1e-81
Identity = 156/170 (91.76%), Postives = 163/170 (95.88%), Query Frame = 0

Query: 1   MLRFSSKRLLGIASGNMAAPAVQVVPRFYHERVIDHYNNPRNVGSFDKNDPTVGTGLVGA 60
           MLR S+KRLLGIASG++ +PA Q+ PRFYHERVIDHYNNPRNVGSFDKNDPTVGTGLVGA
Sbjct: 1   MLRLSTKRLLGIASGDLPSPAFQIFPRFYHERVIDHYNNPRNVGSFDKNDPTVGTGLVGA 60

Query: 61  PACGDVMKLQIKVDEKTGKVVDACFKTFGCGSAIASSSLATEMVKGKQMEEALTIKNSEI 120
           PACGDVMKLQIKVD+KTGKVVDACFKTFGCGSAIASSSLATEMVKGKQMEEALTIKNSEI
Sbjct: 61  PACGDVMKLQIKVDDKTGKVVDACFKTFGCGSAIASSSLATEMVKGKQMEEALTIKNSEI 120

Query: 121 AKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAKLVDGADSPPVVKASSA 171
           AKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAKL DGA+S PV KASSA
Sbjct: 121 AKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAKLADGAESSPVEKASSA 170

BLAST of Carg07677 vs. NCBI nr
Match: XP_008438468.1 (PREDICTED: iron-sulfur cluster assembly protein 1-like [Cucumis melo] >TYJ99158.1 iron-sulfur cluster assembly protein 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 310.1 bits (793), Expect = 1.2e-80
Identity = 157/170 (92.35%), Postives = 164/170 (96.47%), Query Frame = 0

Query: 1   MLRFSSKRLLGIASGNMAAPAVQVVPRFYHERVIDHYNNPRNVGSFDKNDPTVGTGLVGA 60
           MLRFSSKRLLGIAS ++ +PAVQ+VPRFYHERVIDHYNNPRNVGSFDKNDPTVGTGLVGA
Sbjct: 1   MLRFSSKRLLGIASRDLPSPAVQIVPRFYHERVIDHYNNPRNVGSFDKNDPTVGTGLVGA 60

Query: 61  PACGDVMKLQIKVDEKTGKVVDACFKTFGCGSAIASSSLATEMVKGKQMEEALTIKNSEI 120
           PACGDVMKLQIKVDEKTGKVVDACFKTFGCGSAIASSSLATEMVKGKQMEEALTIKNSEI
Sbjct: 61  PACGDVMKLQIKVDEKTGKVVDACFKTFGCGSAIASSSLATEMVKGKQMEEALTIKNSEI 120

Query: 121 AKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAKLVDGADSPPVVKASSA 171
           AKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAKL +GA+S  V KAS+A
Sbjct: 121 AKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAKLANGAESSSVEKASNA 170

BLAST of Carg07677 vs. NCBI nr
Match: XP_004152478.1 (iron-sulfur cluster assembly protein 1 [Cucumis sativus] >KGN64314.1 hypothetical protein Csa_014365 [Cucumis sativus])

HSP 1 Score: 307.0 bits (785), Expect = 1.0e-79
Identity = 154/170 (90.59%), Postives = 163/170 (95.88%), Query Frame = 0

Query: 1   MLRFSSKRLLGIASGNMAAPAVQVVPRFYHERVIDHYNNPRNVGSFDKNDPTVGTGLVGA 60
           MLRFSSKRLLGIAS ++ +PAVQ+VPRFYHERVIDHYNNPRNVGSFDKNDPTVGTGLVGA
Sbjct: 1   MLRFSSKRLLGIASKDLPSPAVQIVPRFYHERVIDHYNNPRNVGSFDKNDPTVGTGLVGA 60

Query: 61  PACGDVMKLQIKVDEKTGKVVDACFKTFGCGSAIASSSLATEMVKGKQMEEALTIKNSEI 120
           PACGDVMKLQIK+DEKTGKVVDACFKTFGCGSAIASSSLATEMVKGKQM+EALTIKNSEI
Sbjct: 61  PACGDVMKLQIKIDEKTGKVVDACFKTFGCGSAIASSSLATEMVKGKQMDEALTIKNSEI 120

Query: 121 AKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAKLVDGADSPPVVKASSA 171
           AKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAKL +GA+S  V K S+A
Sbjct: 121 AKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAKLANGAESSSVEKVSNA 170

BLAST of Carg07677 vs. ExPASy Swiss-Prot
Match: Q8LR34 (Iron-sulfur cluster assembly protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=ISU1 PE=2 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 4.0e-63
Identity = 121/160 (75.62%), Postives = 135/160 (84.38%), Query Frame = 0

Query: 1   MLRFSSKRLLG-----IASGNMAAPAVQVVPRFYHERVIDHYNNPRNVGSFDKNDPTVGT 60
           MLR   +RLL      +  G  AAP      + YHERV+DHY NPRNVGSF+ +DP+VGT
Sbjct: 1   MLRAGGRRLLAPGLRRVLGGGAAAPVAVGGAKAYHERVVDHYENPRNVGSFENDDPSVGT 60

Query: 61  GLVGAPACGDVMKLQIKVDEKTGKVVDACFKTFGCGSAIASSSLATEMVKGKQMEEALTI 120
           GLVGAPACGDVMKLQI+VDE +GK+VDACFKTFGCGSAIASSS+ATE VKGKQMEE +TI
Sbjct: 61  GLVGAPACGDVMKLQIRVDESSGKIVDACFKTFGCGSAIASSSVATEWVKGKQMEEVVTI 120

Query: 121 KNSEIAKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAKL 156
           KN+EIAKHLSLPPVKLHCSMLAEDAIKAAVKDYEAK+AKL
Sbjct: 121 KNTEIAKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKKAKL 160

BLAST of Carg07677 vs. ExPASy Swiss-Prot
Match: O49627 (Iron-sulfur cluster assembly protein 1 OS=Arabidopsis thaliana OX=3702 GN=ISU1 PE=1 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 3.8e-61
Identity = 119/154 (77.27%), Postives = 134/154 (87.01%), Query Frame = 0

Query: 1   MLRFSSKRLLGIASGNMAAPAVQVVPRFYHERVIDHYNNPRNVGSFDKNDPTVGTGLVGA 60
           ML+ ++K+ LG+ S      +V ++ R YHE VIDHY+NPRNVGSFDKNDP VGTGLVGA
Sbjct: 2   MLKQAAKKALGLTSRQSTPWSVGIL-RTYHENVIDHYDNPRNVGSFDKNDPNVGTGLVGA 61

Query: 61  PACGDVMKLQIKVDEKTGKVVDACFKTFGCGSAIASSSLATEMVKGKQMEEALTIKNSEI 120
           PACGDVMKLQIKVDEKTG++VDA FKTFGCGSAIASSS+ATE VKGK ME+ LTIKN+EI
Sbjct: 62  PACGDVMKLQIKVDEKTGQIVDARFKTFGCGSAIASSSVATEWVKGKAMEDVLTIKNTEI 121

Query: 121 AKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAK 155
           AKHLSLPPVKLHCSMLAEDAIKAAVKDY+ KR K
Sbjct: 122 AKHLSLPPVKLHCSMLAEDAIKAAVKDYKEKRVK 154

BLAST of Carg07677 vs. ExPASy Swiss-Prot
Match: O81433 (Iron-sulfur cluster assembly protein 3 OS=Arabidopsis thaliana OX=3702 GN=ISU3 PE=2 SV=1)

HSP 1 Score: 214.9 bits (546), Expect = 6.9e-55
Identity = 112/155 (72.26%), Postives = 129/155 (83.23%), Query Frame = 0

Query: 1   MLRFSSKR-LLGIASGNMAAPAVQVVPRFYHERVIDHYNNPRNVGSFDKNDPTVGTGLVG 60
           MLR ++KR  LG+AS N       VV R YH  VIDHY+NPRNVGSFDKNDP VGTGLVG
Sbjct: 1   MLRQTTKRAFLGLASQN--PTPFPVVSRLYHPNVIDHYDNPRNVGSFDKNDPNVGTGLVG 60

Query: 61  APACGDVMKLQIKVDEKTGKVVDACFKTFGCGSAIASSSLATEMVKGKQMEEALTIKNSE 120
           AP CGDVMKLQ+K D  +G+++DA FKTFGCGSAIA+SS+ATE VKGK +EE LTIKNS+
Sbjct: 61  APQCGDVMKLQVKFD-GSGQIIDAKFKTFGCGSAIAASSVATEWVKGKSVEEVLTIKNSQ 120

Query: 121 IAKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAK 155
           IAKHLSLPPVKLHCSMLAEDAIKAA+K+Y+ K+ K
Sbjct: 121 IAKHLSLPPVKLHCSMLAEDAIKAAIKNYKEKQDK 152

BLAST of Carg07677 vs. ExPASy Swiss-Prot
Match: Q6CRQ9 (Iron sulfur cluster assembly protein 1, mitochondrial OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) OX=284590 GN=ISU1 PE=3 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 6.5e-53
Identity = 103/150 (68.67%), Postives = 122/150 (81.33%), Query Frame = 0

Query: 8   RLLGIASGNMAAPAVQVVP-----RFYHERVIDHYNNPRNVGSFDKNDPTVGTGLVGAPA 67
           R++G+A+    AP V   P     RFYH +VIDHY NPRNVGS DKN P VGTGLVGAPA
Sbjct: 25  RIIGVAAARATAPTVGSSPSMASARFYHPKVIDHYTNPRNVGSLDKNLPNVGTGLVGAPA 84

Query: 68  CGDVMKLQIKVDEKTGKVVDACFKTFGCGSAIASSSLATEMVKGKQMEEALTIKNSEIAK 127
           CGDVMKLQI+V+++TG + +  FKTFGCGSAIASSS  TE+V+GK +E+A  IKN+EIA+
Sbjct: 85  CGDVMKLQIQVNDETGVIENVKFKTFGCGSAIASSSYMTELVRGKTLEDAAKIKNTEIAR 144

Query: 128 HLSLPPVKLHCSMLAEDAIKAAVKDYEAKR 153
            LSLPPVKLHCSMLAEDAIKAA+KDY+AKR
Sbjct: 145 ELSLPPVKLHCSMLAEDAIKAAIKDYQAKR 174

BLAST of Carg07677 vs. ExPASy Swiss-Prot
Match: Q9H1K1 (Iron-sulfur cluster assembly enzyme ISCU, mitochondrial OS=Homo sapiens OX=9606 GN=ISCU PE=1 SV=2)

HSP 1 Score: 203.0 bits (515), Expect = 2.7e-51
Identity = 106/151 (70.20%), Postives = 122/151 (80.79%), Query Frame = 0

Query: 2   LRFSSKRLLGIASGNMAAPAVQVVPRFYHERVIDHYNNPRNVGSFDKNDPTVGTGLVGAP 61
           LR ++  LL + S  + A  +    R YH++V+DHY NPRNVGS DK    VGTGLVGAP
Sbjct: 9   LRRAASALL-LRSPRLPARELSAPARLYHKKVVDHYENPRNVGSLDKTSKNVGTGLVGAP 68

Query: 62  ACGDVMKLQIKVDEKTGKVVDACFKTFGCGSAIASSSLATEMVKGKQMEEALTIKNSEIA 121
           ACGDVMKLQI+VDEK GK+VDA FKTFGCGSAIASSSLATE VKGK +EEALTIKN++IA
Sbjct: 69  ACGDVMKLQIQVDEK-GKIVDARFKTFGCGSAIASSSLATEWVKGKTVEEALTIKNTDIA 128

Query: 122 KHLSLPPVKLHCSMLAEDAIKAAVKDYEAKR 153
           K L LPPVKLHCSMLAEDAIKAA+ DY+ K+
Sbjct: 129 KELCLPPVKLHCSMLAEDAIKAALADYKLKQ 157

BLAST of Carg07677 vs. ExPASy TrEMBL
Match: A0A6J1CB43 (Iron-sulfur cluster assembly protein OS=Momordica charantia OX=3673 GN=LOC111009078 PE=3 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 3.4e-81
Identity = 156/170 (91.76%), Postives = 163/170 (95.88%), Query Frame = 0

Query: 1   MLRFSSKRLLGIASGNMAAPAVQVVPRFYHERVIDHYNNPRNVGSFDKNDPTVGTGLVGA 60
           MLR S+KRLLGIASG++ +PA Q+ PRFYHERVIDHYNNPRNVGSFDKNDPTVGTGLVGA
Sbjct: 1   MLRLSTKRLLGIASGDLPSPAFQIFPRFYHERVIDHYNNPRNVGSFDKNDPTVGTGLVGA 60

Query: 61  PACGDVMKLQIKVDEKTGKVVDACFKTFGCGSAIASSSLATEMVKGKQMEEALTIKNSEI 120
           PACGDVMKLQIKVD+KTGKVVDACFKTFGCGSAIASSSLATEMVKGKQMEEALTIKNSEI
Sbjct: 61  PACGDVMKLQIKVDDKTGKVVDACFKTFGCGSAIASSSLATEMVKGKQMEEALTIKNSEI 120

Query: 121 AKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAKLVDGADSPPVVKASSA 171
           AKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAKL DGA+S PV KASSA
Sbjct: 121 AKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAKLADGAESSPVEKASSA 170

BLAST of Carg07677 vs. ExPASy TrEMBL
Match: A0A5D3BLW4 (Iron-sulfur cluster assembly protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G003660 PE=3 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 5.8e-81
Identity = 157/170 (92.35%), Postives = 164/170 (96.47%), Query Frame = 0

Query: 1   MLRFSSKRLLGIASGNMAAPAVQVVPRFYHERVIDHYNNPRNVGSFDKNDPTVGTGLVGA 60
           MLRFSSKRLLGIAS ++ +PAVQ+VPRFYHERVIDHYNNPRNVGSFDKNDPTVGTGLVGA
Sbjct: 1   MLRFSSKRLLGIASRDLPSPAVQIVPRFYHERVIDHYNNPRNVGSFDKNDPTVGTGLVGA 60

Query: 61  PACGDVMKLQIKVDEKTGKVVDACFKTFGCGSAIASSSLATEMVKGKQMEEALTIKNSEI 120
           PACGDVMKLQIKVDEKTGKVVDACFKTFGCGSAIASSSLATEMVKGKQMEEALTIKNSEI
Sbjct: 61  PACGDVMKLQIKVDEKTGKVVDACFKTFGCGSAIASSSLATEMVKGKQMEEALTIKNSEI 120

Query: 121 AKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAKLVDGADSPPVVKASSA 171
           AKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAKL +GA+S  V KAS+A
Sbjct: 121 AKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAKLANGAESSSVEKASNA 170

BLAST of Carg07677 vs. ExPASy TrEMBL
Match: A0A1S3AX35 (Iron-sulfur cluster assembly protein OS=Cucumis melo OX=3656 GN=LOC103483550 PE=3 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 5.8e-81
Identity = 157/170 (92.35%), Postives = 164/170 (96.47%), Query Frame = 0

Query: 1   MLRFSSKRLLGIASGNMAAPAVQVVPRFYHERVIDHYNNPRNVGSFDKNDPTVGTGLVGA 60
           MLRFSSKRLLGIAS ++ +PAVQ+VPRFYHERVIDHYNNPRNVGSFDKNDPTVGTGLVGA
Sbjct: 1   MLRFSSKRLLGIASRDLPSPAVQIVPRFYHERVIDHYNNPRNVGSFDKNDPTVGTGLVGA 60

Query: 61  PACGDVMKLQIKVDEKTGKVVDACFKTFGCGSAIASSSLATEMVKGKQMEEALTIKNSEI 120
           PACGDVMKLQIKVDEKTGKVVDACFKTFGCGSAIASSSLATEMVKGKQMEEALTIKNSEI
Sbjct: 61  PACGDVMKLQIKVDEKTGKVVDACFKTFGCGSAIASSSLATEMVKGKQMEEALTIKNSEI 120

Query: 121 AKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAKLVDGADSPPVVKASSA 171
           AKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAKL +GA+S  V KAS+A
Sbjct: 121 AKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAKLANGAESSSVEKASNA 170

BLAST of Carg07677 vs. ExPASy TrEMBL
Match: A0A5A7UJ68 (Iron-sulfur cluster assembly protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold80G00720 PE=3 SV=1)

HSP 1 Score: 307.0 bits (785), Expect = 4.9e-80
Identity = 155/170 (91.18%), Postives = 163/170 (95.88%), Query Frame = 0

Query: 1   MLRFSSKRLLGIASGNMAAPAVQVVPRFYHERVIDHYNNPRNVGSFDKNDPTVGTGLVGA 60
           MLRFSSKRLLGIAS ++ +PAVQ+VPRFYHERVIDHYNNPRNVGSFDKNDPTVGTGLVGA
Sbjct: 1   MLRFSSKRLLGIASRDLPSPAVQIVPRFYHERVIDHYNNPRNVGSFDKNDPTVGTGLVGA 60

Query: 61  PACGDVMKLQIKVDEKTGKVVDACFKTFGCGSAIASSSLATEMVKGKQMEEALTIKNSEI 120
           PACGDVMKLQIKVDEKTGKVVDACFKTFGCGSAIASSS ATEMVKGKQMEEALTIKNSEI
Sbjct: 61  PACGDVMKLQIKVDEKTGKVVDACFKTFGCGSAIASSSFATEMVKGKQMEEALTIKNSEI 120

Query: 121 AKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAKLVDGADSPPVVKASSA 171
           AKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAKL +GA+S  V +AS+A
Sbjct: 121 AKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAKLANGAESSSVEEASNA 170

BLAST of Carg07677 vs. ExPASy TrEMBL
Match: A0A0A0LTI4 (Iron-sulfur cluster assembly protein OS=Cucumis sativus OX=3659 GN=Csa_1G046100 PE=3 SV=1)

HSP 1 Score: 307.0 bits (785), Expect = 4.9e-80
Identity = 154/170 (90.59%), Postives = 163/170 (95.88%), Query Frame = 0

Query: 1   MLRFSSKRLLGIASGNMAAPAVQVVPRFYHERVIDHYNNPRNVGSFDKNDPTVGTGLVGA 60
           MLRFSSKRLLGIAS ++ +PAVQ+VPRFYHERVIDHYNNPRNVGSFDKNDPTVGTGLVGA
Sbjct: 1   MLRFSSKRLLGIASKDLPSPAVQIVPRFYHERVIDHYNNPRNVGSFDKNDPTVGTGLVGA 60

Query: 61  PACGDVMKLQIKVDEKTGKVVDACFKTFGCGSAIASSSLATEMVKGKQMEEALTIKNSEI 120
           PACGDVMKLQIK+DEKTGKVVDACFKTFGCGSAIASSSLATEMVKGKQM+EALTIKNSEI
Sbjct: 61  PACGDVMKLQIKIDEKTGKVVDACFKTFGCGSAIASSSLATEMVKGKQMDEALTIKNSEI 120

Query: 121 AKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAKLVDGADSPPVVKASSA 171
           AKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAKL +GA+S  V K S+A
Sbjct: 121 AKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAKLANGAESSSVEKVSNA 170

BLAST of Carg07677 vs. TAIR 10
Match: AT4G22220.1 (SufE/NifU family protein )

HSP 1 Score: 235.7 bits (600), Expect = 2.7e-62
Identity = 119/154 (77.27%), Postives = 134/154 (87.01%), Query Frame = 0

Query: 1   MLRFSSKRLLGIASGNMAAPAVQVVPRFYHERVIDHYNNPRNVGSFDKNDPTVGTGLVGA 60
           ML+ ++K+ LG+ S      +V ++ R YHE VIDHY+NPRNVGSFDKNDP VGTGLVGA
Sbjct: 2   MLKQAAKKALGLTSRQSTPWSVGIL-RTYHENVIDHYDNPRNVGSFDKNDPNVGTGLVGA 61

Query: 61  PACGDVMKLQIKVDEKTGKVVDACFKTFGCGSAIASSSLATEMVKGKQMEEALTIKNSEI 120
           PACGDVMKLQIKVDEKTG++VDA FKTFGCGSAIASSS+ATE VKGK ME+ LTIKN+EI
Sbjct: 62  PACGDVMKLQIKVDEKTGQIVDARFKTFGCGSAIASSSVATEWVKGKAMEDVLTIKNTEI 121

Query: 121 AKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAK 155
           AKHLSLPPVKLHCSMLAEDAIKAAVKDY+ KR K
Sbjct: 122 AKHLSLPPVKLHCSMLAEDAIKAAVKDYKEKRVK 154

BLAST of Carg07677 vs. TAIR 10
Match: AT4G04080.1 (ISCU-like 3 )

HSP 1 Score: 214.9 bits (546), Expect = 4.9e-56
Identity = 112/155 (72.26%), Postives = 129/155 (83.23%), Query Frame = 0

Query: 1   MLRFSSKR-LLGIASGNMAAPAVQVVPRFYHERVIDHYNNPRNVGSFDKNDPTVGTGLVG 60
           MLR ++KR  LG+AS N       VV R YH  VIDHY+NPRNVGSFDKNDP VGTGLVG
Sbjct: 1   MLRQTTKRAFLGLASQN--PTPFPVVSRLYHPNVIDHYDNPRNVGSFDKNDPNVGTGLVG 60

Query: 61  APACGDVMKLQIKVDEKTGKVVDACFKTFGCGSAIASSSLATEMVKGKQMEEALTIKNSE 120
           AP CGDVMKLQ+K D  +G+++DA FKTFGCGSAIA+SS+ATE VKGK +EE LTIKNS+
Sbjct: 61  APQCGDVMKLQVKFD-GSGQIIDAKFKTFGCGSAIAASSVATEWVKGKSVEEVLTIKNSQ 120

Query: 121 IAKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAK 155
           IAKHLSLPPVKLHCSMLAEDAIKAA+K+Y+ K+ K
Sbjct: 121 IAKHLSLPPVKLHCSMLAEDAIKAAIKNYKEKQDK 152

BLAST of Carg07677 vs. TAIR 10
Match: AT3G01020.1 (ISCU-like 2 )

HSP 1 Score: 202.2 bits (513), Expect = 3.3e-52
Identity = 105/167 (62.87%), Postives = 133/167 (79.64%), Query Frame = 0

Query: 1   MLRFSSKRLLGIASGNMAAPAVQVVPRFYHERVIDHYNNPRNVGSFDKNDPTVGTGLVGA 60
           MLR +S++    A   + A  + +  R YHE VIDH+ NPRNVGSF++NDP VGTGLVGA
Sbjct: 3   MLRQTSRK----AYLGLQASPLGLGRRLYHENVIDHFENPRNVGSFNRNDPNVGTGLVGA 62

Query: 61  PACGDVMKLQIKVDEKTGKVVDACFKTFGCGSAIASSSLATEMVKGKQMEEALTIKNSEI 120
           PACGD+M LQIKVD+ +G+++D  FKTFGCGSAIASSS+A+E +KGK ++E +TIKN+EI
Sbjct: 63  PACGDLMSLQIKVDD-SGQIIDTRFKTFGCGSAIASSSVASEWIKGKTLDEVMTIKNAEI 122

Query: 121 AKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKRAKLVDGADSPPVVKA 168
           AKHL LPPVKLHCSMLAEDAIK+AV+DY+ K+AK  + A +   VKA
Sbjct: 123 AKHLRLPPVKLHCSMLAEDAIKSAVRDYKEKQAK-TNAAAAEETVKA 163

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6584219.16.0e-88100.00Iron-sulfur cluster assembly protein 1, partial [Cucurbita argyrosperma subsp. s... [more]
XP_038895391.13.2e-8192.94iron-sulfur cluster assembly protein 1-like [Benincasa hispida][more]
XP_022137708.17.1e-8191.76uncharacterized protein LOC111009078 [Momordica charantia][more]
XP_008438468.11.2e-8092.35PREDICTED: iron-sulfur cluster assembly protein 1-like [Cucumis melo] >TYJ99158.... [more]
XP_004152478.11.0e-7990.59iron-sulfur cluster assembly protein 1 [Cucumis sativus] >KGN64314.1 hypothetica... [more]
Match NameE-valueIdentityDescription
Q8LR344.0e-6375.63Iron-sulfur cluster assembly protein 1 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
O496273.8e-6177.27Iron-sulfur cluster assembly protein 1 OS=Arabidopsis thaliana OX=3702 GN=ISU1 P... [more]
O814336.9e-5572.26Iron-sulfur cluster assembly protein 3 OS=Arabidopsis thaliana OX=3702 GN=ISU3 P... [more]
Q6CRQ96.5e-5368.67Iron sulfur cluster assembly protein 1, mitochondrial OS=Kluyveromyces lactis (s... [more]
Q9H1K12.7e-5170.20Iron-sulfur cluster assembly enzyme ISCU, mitochondrial OS=Homo sapiens OX=9606 ... [more]
Match NameE-valueIdentityDescription
A0A6J1CB433.4e-8191.76Iron-sulfur cluster assembly protein OS=Momordica charantia OX=3673 GN=LOC111009... [more]
A0A5D3BLW45.8e-8192.35Iron-sulfur cluster assembly protein OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3AX355.8e-8192.35Iron-sulfur cluster assembly protein OS=Cucumis melo OX=3656 GN=LOC103483550 PE=... [more]
A0A5A7UJ684.9e-8091.18Iron-sulfur cluster assembly protein OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A0A0LTI44.9e-8090.59Iron-sulfur cluster assembly protein OS=Cucumis sativus OX=3659 GN=Csa_1G046100 ... [more]
Match NameE-valueIdentityDescription
AT4G22220.12.7e-6277.27SufE/NifU family protein [more]
AT4G04080.14.9e-5672.26ISCU-like 3 [more]
AT3G01020.13.3e-5262.87ISCU-like 2 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.90.1010.10coord: 28..162
e-value: 1.0E-50
score: 173.7
NoneNo IPR availablePANTHERPTHR10093:SF20IRON-SULFUR CLUSTER ASSEMBLY PROTEINcoord: 118..161
coord: 1..118
NoneNo IPR availableSUPERFAMILY82649SufE/NifUcoord: 28..152
IPR002871NIF system FeS cluster assembly, NifU, N-terminalPFAMPF01592NifU_Ncoord: 29..153
e-value: 1.3E-57
score: 193.5
IPR002871NIF system FeS cluster assembly, NifU, N-terminalPANTHERPTHR10093IRON-SULFUR CLUSTER ASSEMBLY ENZYME NIFU HOMOLOGcoord: 118..161
IPR002871NIF system FeS cluster assembly, NifU, N-terminalPANTHERPTHR10093IRON-SULFUR CLUSTER ASSEMBLY ENZYME NIFU HOMOLOGcoord: 1..118
IPR002871NIF system FeS cluster assembly, NifU, N-terminalCDDcd06664IscU_likecoord: 29..144
e-value: 1.20767E-48
score: 151.633
IPR011339ISC system FeS cluster assembly, IscU scaffoldTIGRFAMTIGR01999TIGR01999coord: 29..152
e-value: 1.9E-65
score: 216.6

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg07677-RACarg07677-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006879 cellular iron ion homeostasis
biological_process GO:0016226 iron-sulfur cluster assembly
cellular_component GO:0005759 mitochondrial matrix
molecular_function GO:0051537 2 iron, 2 sulfur cluster binding
molecular_function GO:0008198 ferrous iron binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0051536 iron-sulfur cluster binding