Carg07589 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg07589 vs. NCBI nr
Match: KAG7019731.1 (4-coumarate--CoA ligase-like 9 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1082.4 bits (2798), Expect = 0.0e+00
Identity = 557/557 (100.00%), Postives = 557/557 (100.00%), Query Frame = 0

Query: 1   MADRNPSLNAAHFVDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPS 60
           MADRNPSLNAAHFVDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPS
Sbjct: 1   MADRNPSLNAAHFVDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPS 60

Query: 61  NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL 120
           NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL
Sbjct: 61  NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL 120

Query: 121 SLGVVISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLPLGTVLIDSPAFLSMMT 180
           SLGVVISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLPLGTVLIDSPAFLSMMT
Sbjct: 121 SLGVVISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLPLGTVLIDSPAFLSMMT 180

Query: 181 EINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET 240
           EINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET
Sbjct: 181 EINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET 240

Query: 241 EPRRVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPL 300
           EPRRVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPL
Sbjct: 241 EPRRVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPL 300

Query: 301 VVAMAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR 360
           VVAMAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR
Sbjct: 301 VVAMAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR 360

Query: 361 SMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKATT 420
           SMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKATT
Sbjct: 361 SMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKATT 420

Query: 421 ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV 480
           ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV
Sbjct: 421 ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV 480

Query: 481 IPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI 540
           IPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI
Sbjct: 481 IPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI 540

Query: 541 LRRELTQHARSHGSNKL 558
           LRRELTQHARSHGSNKL
Sbjct: 541 LRRELTQHARSHGSNKL 557

BLAST of Carg07589 vs. NCBI nr
Match: KAG6584140.1 (4-coumarate--CoA ligase-like 9, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1080.9 bits (2794), Expect = 0.0e+00
Identity = 556/557 (99.82%), Postives = 557/557 (100.00%), Query Frame = 0

Query: 1   MADRNPSLNAAHFVDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPS 60
           MADRNPSLNAAHFVDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPS
Sbjct: 1   MADRNPSLNAAHFVDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPS 60

Query: 61  NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL 120
           NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL
Sbjct: 61  NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL 120

Query: 121 SLGVVISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLPLGTVLIDSPAFLSMMT 180
           SLGVVISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLPLGTVLIDSPAFLSMMT
Sbjct: 121 SLGVVISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLPLGTVLIDSPAFLSMMT 180

Query: 181 EINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET 240
           EINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET
Sbjct: 181 EINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET 240

Query: 241 EPRRVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPL 300
           EPR+VSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPL
Sbjct: 241 EPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPL 300

Query: 301 VVAMAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR 360
           VVAMAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR
Sbjct: 301 VVAMAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR 360

Query: 361 SMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKATT 420
           SMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKATT
Sbjct: 361 SMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKATT 420

Query: 421 ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV 480
           ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV
Sbjct: 421 ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV 480

Query: 481 IPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI 540
           IPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI
Sbjct: 481 IPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI 540

Query: 541 LRRELTQHARSHGSNKL 558
           LRRELTQHARSHGSNKL
Sbjct: 541 LRRELTQHARSHGSNKL 557

BLAST of Carg07589 vs. NCBI nr
Match: XP_022923628.1 (4-coumarate--CoA ligase-like 9 [Cucurbita moschata])

HSP 1 Score: 1076.2 bits (2782), Expect = 0.0e+00
Identity = 554/557 (99.46%), Postives = 556/557 (99.82%), Query Frame = 0

Query: 1   MADRNPSLNAAHFVDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPS 60
           MADRNPSLNAAHFVDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPS
Sbjct: 1   MADRNPSLNAAHFVDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPS 60

Query: 61  NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL 120
           NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL
Sbjct: 61  NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL 120

Query: 121 SLGVVISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLPLGTVLIDSPAFLSMMT 180
           SLGVVISPANPIGSDSEISHQIRLS+PVIAFATSSTASKLPKL LGTVLIDSPAFLSMMT
Sbjct: 121 SLGVVISPANPIGSDSEISHQIRLSKPVIAFATSSTASKLPKLQLGTVLIDSPAFLSMMT 180

Query: 181 EINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET 240
           EINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET
Sbjct: 181 EINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET 240

Query: 241 EPRRVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPL 300
           EPR+VSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPL
Sbjct: 241 EPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPL 300

Query: 301 VVAMAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR 360
           VVAMAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR
Sbjct: 301 VVAMAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR 360

Query: 361 SMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKATT 420
           SMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKATT
Sbjct: 361 SMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKATT 420

Query: 421 ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV 480
           ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV
Sbjct: 421 ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV 480

Query: 481 IPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI 540
           IPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI
Sbjct: 481 IPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI 540

Query: 541 LRRELTQHARSHGSNKL 558
           LRRELTQHARSHGSNKL
Sbjct: 541 LRRELTQHARSHGSNKL 557

BLAST of Carg07589 vs. NCBI nr
Match: XP_023519270.1 (4-coumarate--CoA ligase-like 9 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1067.0 bits (2758), Expect = 5.5e-308
Identity = 549/557 (98.56%), Postives = 554/557 (99.46%), Query Frame = 0

Query: 1   MADRNPSLNAAHFVDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPS 60
           MADRNPSLNAAHFVD RSGFCPQTKIF SLRPPL+IPPLSQPLSVTEHALSLLQSSPPPS
Sbjct: 1   MADRNPSLNAAHFVDIRSGFCPQTKIFRSLRPPLIIPPLSQPLSVTEHALSLLQSSPPPS 60

Query: 61  NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL 120
           NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL
Sbjct: 61  NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL 120

Query: 121 SLGVVISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLPLGTVLIDSPAFLSMMT 180
           SLGVVISPANPIGSDSEISHQIRLS+PVIAFATSSTASKLPKLPLGTVLIDSPAFLSMMT
Sbjct: 121 SLGVVISPANPIGSDSEISHQIRLSQPVIAFATSSTASKLPKLPLGTVLIDSPAFLSMMT 180

Query: 181 EINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET 240
           EINRSDG+ADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET
Sbjct: 181 EINRSDGIADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET 240

Query: 241 EPRRVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPL 300
           EPR+VSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKF VTYIPVSPPL
Sbjct: 241 EPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFGVTYIPVSPPL 300

Query: 301 VVAMAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR 360
           VVA+AKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR
Sbjct: 301 VVALAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR 360

Query: 361 SMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKATT 420
           SMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKATT
Sbjct: 361 SMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKATT 420

Query: 421 ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV 480
           ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV
Sbjct: 421 ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV 480

Query: 481 IPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI 540
           IPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI
Sbjct: 481 IPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI 540

Query: 541 LRRELTQHARSHGSNKL 558
           LRRELTQHARSHGSNKL
Sbjct: 541 LRRELTQHARSHGSNKL 557

BLAST of Carg07589 vs. NCBI nr
Match: XP_023000795.1 (4-coumarate--CoA ligase-like 9 [Cucurbita maxima])

HSP 1 Score: 1063.1 bits (2748), Expect = 8.0e-307
Identity = 548/557 (98.38%), Postives = 552/557 (99.10%), Query Frame = 0

Query: 1   MADRNPSLNAAHFVDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPS 60
           MADRNPSLNAAHFVD RSGFCPQTKIF SLRPPLLIPPLSQPLSVTEHALSLLQSSPPPS
Sbjct: 1   MADRNPSLNAAHFVDLRSGFCPQTKIFRSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPS 60

Query: 61  NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL 120
           NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL
Sbjct: 61  NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL 120

Query: 121 SLGVVISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLPLGTVLIDSPAFLSMMT 180
           SLGV+ISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKL LGTVLIDSPAFLSMMT
Sbjct: 121 SLGVIISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLQLGTVLIDSPAFLSMMT 180

Query: 181 EINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET 240
           EINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET
Sbjct: 181 EINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET 240

Query: 241 EPRRVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPL 300
           EPR+VSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPL
Sbjct: 241 EPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPL 300

Query: 301 VVAMAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR 360
           VVA+AKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR
Sbjct: 301 VVALAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR 360

Query: 361 SMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKATT 420
           SMGPEESSNTKSVGRLSENMEAMIVDP SGEALPPGHRGELWLRGPGIMKGYVGDA ATT
Sbjct: 361 SMGPEESSNTKSVGRLSENMEAMIVDPASGEALPPGHRGELWLRGPGIMKGYVGDADATT 420

Query: 421 ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV 480
           ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV
Sbjct: 421 ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV 480

Query: 481 IPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI 540
           IPYPDEDAGEIPMAYVVRKPGSN+TEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI
Sbjct: 481 IPYPDEDAGEIPMAYVVRKPGSNVTEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI 540

Query: 541 LRRELTQHARSHGSNKL 558
           LRRELTQHARSHGSNKL
Sbjct: 541 LRRELTQHARSHGSNKL 557

BLAST of Carg07589 vs. ExPASy Swiss-Prot
Match: Q84P23 (4-coumarate--CoA ligase-like 9 OS=Arabidopsis thaliana OX=3702 GN=4CLL9 PE=1 SV=2)

HSP 1 Score: 669.5 bits (1726), Expect = 3.4e-191
Identity = 356/565 (63.01%), Postives = 436/565 (77.17%), Query Frame = 0

Query: 9   NAAHFVDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPSNA------ 68
           N +  +D  SGF  +T I+HSLRP L +PP+ QPLS  E ALSLL  S PP+ A      
Sbjct: 6   NDSRLIDRSSGFDQRTGIYHSLRPSLSLPPIDQPLSAAEFALSLLLKSSPPATAGKNIEA 65

Query: 69  -AVLVDSNSGVHLSYAIFLRQIRTLASNLK-ALTSLSNGQVAFILAPTSLEVPVLYFALL 128
              LV+S+SG +L+Y   LR++R+LA +L+    SL++  VAFIL+P+SL++PVLY AL+
Sbjct: 66  LTYLVNSSSGDNLTYGELLRRVRSLAVSLRERFPSLASRNVAFILSPSSLDIPVLYLALM 125

Query: 129 SLGVVISPANPIGSDSEISHQIRLSEPVIAFATSSTASKL--PKLPLGTVLIDSPAFLSM 188
           S+GVV+SPANPIGS+SE+SHQ+ +SEPVIAFATS T  KL    LPLGTVL+DS  FLS 
Sbjct: 126 SIGVVVSPANPIGSESEVSHQVEVSEPVIAFATSQTVKKLQSSSLPLGTVLMDSTEFLSW 185

Query: 189 MTEINRSDGLA----DVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVN--SSPNPLL 248
              +NRSD  +     V+V+QSD AAIL+SSGTTGRVKGVLL+HRNLI     S    L 
Sbjct: 186 ---LNRSDSSSVNPFQVQVNQSDPAAILFSSGTTGRVKGVLLTHRNLIASTAVSHQRTLQ 245

Query: 249 SEVNNGETEPRRVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVT 308
             VN       RV L  LPLFHVFGF+M++RAIS GETLVL+ +F+   M  AVEK++VT
Sbjct: 246 DPVNYD-----RVGLFSLPLFHVFGFMMMIRAISLGETLVLLGRFELEAMFKAVEKYKVT 305

Query: 309 YIPVSPPLVVAMAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLT 368
            +PVSPPL+VA+ KSEL  KYDL SL+ LGCGGAPLGK++ E+F QK P V+I+QGYGLT
Sbjct: 306 GMPVSPPLIVALVKSELTKKYDLRSLRSLGCGGAPLGKDIAERFKQKFPDVDIVQGYGLT 365

Query: 369 ESTAGAARSMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGY 428
           ES+  AA + GPEE     SVGR+SENMEA IVDP +GE+LPPG  GELWLRGP IMKGY
Sbjct: 366 ESSGPAASTFGPEEMVKYGSVGRISENMEAKIVDPSTGESLPPGKTGELWLRGPVIMKGY 425

Query: 429 VGDAKATTETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSN 488
           VG+ KA+ ET+  +GWLKTGDLCYFDS+ FLYIVDRLKELIKYKAYQVPP ELE +L SN
Sbjct: 426 VGNEKASAETVDKEGWLKTGDLCYFDSEDFLYIVDRLKELIKYKAYQVPPVELEQILHSN 485

Query: 489 PEIIDAAVIPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAI 548
           P++IDAAV+P+PDEDAGEIPMA++VRKPGSN+ EAQ+IDF+AKQV PYKK+RRV+FI+AI
Sbjct: 486 PDVIDAAVVPFPDEDAGEIPMAFIVRKPGSNLNEAQIIDFVAKQVTPYKKVRRVAFINAI 545

Query: 549 PKSPAGKILRRELTQHARSHGSNKL 558
           PK+PAGKILRRELT+ A    ++KL
Sbjct: 546 PKNPAGKILRRELTKIAVDGNASKL 562

BLAST of Carg07589 vs. ExPASy Swiss-Prot
Match: Q7F1X5 (4-coumarate--CoA ligase-like 5 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL5 PE=2 SV=1)

HSP 1 Score: 556.6 bits (1433), Expect = 3.2e-157
Identity = 304/565 (53.81%), Postives = 392/565 (69.38%), Query Frame = 0

Query: 1   MADRNPSLNAAHF-VDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPP 60
           MADR P        +D RSGFC  T+IFHS R P  +PP S P++   +A SLL SS  P
Sbjct: 1   MADRAPPPPPPPAGIDSRSGFCAATRIFHSTRAPGDLPPESLPMTAAAYAFSLLSSSTLP 60

Query: 61  SNAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFAL 120
              A LVD+ +G+ +SY  FL  +R+LA  L     L  G VA ++AP+ LEVPVL FAL
Sbjct: 61  GRPA-LVDAATGIAISYPSFLAAVRSLAGGLWCSLGLRPGDVALVVAPSRLEVPVLDFAL 120

Query: 121 LSLGVVISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLPLGTVLIDSPAFLSM- 180
           +S+G V+SPANP+ +  E +HQ+ LS PV+AFA    A+KLP+  +  V+I S  +  + 
Sbjct: 121 MSIGAVVSPANPVSTPEEYAHQVALSRPVVAFAAPEVAAKLPE-HVRCVVIGSDEYGRLA 180

Query: 181 MTEINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNG 240
            ++  R+   A V V QSD+AA+LYSSGTTGRVK V ++HRNLI + S       +V   
Sbjct: 181 ASDGRRAAAPAAVAVKQSDTAAVLYSSGTTGRVKAVAITHRNLIALMSLHADNREKVARE 240

Query: 241 ETE------PRRVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVT 300
             E      P  V+L  +PLFHVFGF+M+LR++S GET VLM++FDF+  L A+E++RVT
Sbjct: 241 AAEAGEEPPPPAVTLLPIPLFHVFGFMMVLRSVSMGETSVLMERFDFIAALRAIERYRVT 300

Query: 301 YIPVSPPLVVAMAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLT 360
            +P +PP++VAM K E   + DLSSL V+G GGAPLG+EV E+F    P VE++QGYGLT
Sbjct: 301 LLPAAPPVLVAMVKYEEARRRDLSSLLVIGIGGAPLGREVAEQFASVFPNVELVQGYGLT 360

Query: 361 ESTAGAARSMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGY 420
           ES+   A ++GPEES    SVG+L  +++A IVDP +                     GY
Sbjct: 361 ESSGAVAATVGPEESKAYGSVGKLGSHLQAKIVDPST---------------------GY 420

Query: 421 VGDAKATTETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSN 480
           VGD +AT  T+  +GWLKTGDLCYF+ DGFLYIVDRLKELIKYK YQVPPAELEH+LQS+
Sbjct: 421 VGDDEATAATVDSEGWLKTGDLCYFNEDGFLYIVDRLKELIKYKGYQVPPAELEHILQSH 480

Query: 481 PEIIDAAVIPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAI 540
           P I DAAVIPYPDE+AGE+PMA++VR+PGSNIT+ QV+D++AKQVAPYKK+RRV+F+ AI
Sbjct: 481 PGIADAAVIPYPDEEAGELPMAFIVRQPGSNITKEQVMDYVAKQVAPYKKVRRVAFVTAI 540

Query: 541 PKSPAGKILRRELTQHARSHGSNKL 558
           PKSPAGKILRREL Q A S G++KL
Sbjct: 541 PKSPAGKILRRELVQQALSMGASKL 542

BLAST of Carg07589 vs. ExPASy Swiss-Prot
Match: Q8RU95 (4-coumarate--CoA ligase-like 6 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL6 PE=2 SV=2)

HSP 1 Score: 546.2 bits (1406), Expect = 4.3e-154
Identity = 293/558 (52.51%), Postives = 380/558 (68.10%), Query Frame = 0

Query: 17  RSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPP---PSNAAVLVDSNSGVH 76
           RSGFC  T+ FHSLR    +PP   PL+V  +A SLL S+PP         LVD+ +G+ 
Sbjct: 41  RSGFCAATRTFHSLRSVGPLPPEELPLTVAAYAFSLLSSAPPLVVAGRGPALVDAATGIA 100

Query: 77  LSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALLSLGVVISPANPIG 136
           +SY  F+ ++R LA  L     L  G VA +++P+ L+V VLYFAL+S+GVV+SPANP  
Sbjct: 101 VSYPAFVARVRFLAGGLWCSLGLRPGDVALVVSPSCLDVAVLYFALMSIGVVVSPANPAS 160

Query: 137 SDSEISHQIRLSEPVIAFATSSTASKLPKLPLGTVLIDSPAFLSMMTEINRSDGLA---D 196
           +  E +HQ+RLS P IAF     A++LP+  +  V+I S  F   +   + + G A    
Sbjct: 161 TADEYAHQVRLSRPAIAFVAPEVAARLPR-HVSRVVIGSEVF-DRLASASAAGGWAAPPA 220

Query: 197 VKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNN------------- 256
           V + Q  +AA+LYSSGTTGRVK V ++HRNLI   S+ N +   V               
Sbjct: 221 VAMKQPSTAALLYSSGTTGRVKAVAITHRNLIAQISAYNAIRETVAREAATDAGKGKPPP 280

Query: 257 -GETEPRRVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPV 316
              + P  V+L  LPLFHV GF +L R IS GET V+M++FD      AVE++RVT +  
Sbjct: 281 PSPSPPAAVTLFPLPLFHVMGFGLLTRTISSGETAVVMRRFDLAAAARAVERYRVTKLSA 340

Query: 317 SPPLVVAMAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTA 376
           +PP+VVA+ KS+   + DLSSL  +  GGAPLG+EV ++F    P V+I+Q YGLTEST 
Sbjct: 341 APPVVVALTKSDEARRRDLSSLVAIVVGGAPLGREVSQRFATVFPSVQIVQSYGLTESTG 400

Query: 377 GAARSMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDA 436
             A   GPEES+   SVGRL+  ++A IVD  +GE L PG RGELW+RGP +MKGYVGD 
Sbjct: 401 PVATMAGPEESAAYGSVGRLAPRVQAKIVDTATGEVLGPGRRGELWIRGPVVMKGYVGDP 460

Query: 437 KATTETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEII 496
           +AT  T+ PDGWLKTGDLCYF+ DG+LY+VDRLKELIKYK YQVPPAELEH+LQS PEI 
Sbjct: 461 EATAATITPDGWLKTGDLCYFNEDGYLYVVDRLKELIKYKGYQVPPAELEHILQSRPEIA 520

Query: 497 DAAVIPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSP 555
           DAAV+PYPDE+AG++PMA+VVR+PG+ +TE QV++ +AK VAPYKK+RRV+F++AIPKSP
Sbjct: 521 DAAVVPYPDEEAGQLPMAFVVRQPGAYLTEQQVMNCVAKHVAPYKKVRRVAFVNAIPKSP 580

BLAST of Carg07589 vs. ExPASy Swiss-Prot
Match: Q69RG7 (4-coumarate--CoA ligase-like 7 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL7 PE=2 SV=1)

HSP 1 Score: 485.0 bits (1247), Expect = 1.2e-135
Identity = 266/547 (48.63%), Postives = 366/547 (66.91%), Query Frame = 0

Query: 17  RSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPSNAAV------LVDSNS 76
           R+G+C  TK F SLRPP+ +PP   PLS  E A SLL  S   S++++      LVD+ +
Sbjct: 16  RTGYCAATKSFRSLRPPVPLPPPDVPLSFPEFAFSLLPRSSSSSSSSLLPANPALVDAAT 75

Query: 77  GVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALLSLGVVISPAN 136
           G  +S+  FL ++R LA  L++   L  G VAF+LAP  L+VPVLYFALLS+G V+SPAN
Sbjct: 76  GEAVSFQAFLSRVRALAGALRSRVGLRGGDVAFVLAPAGLDVPVLYFALLSIGAVVSPAN 135

Query: 137 PIGSDSEISHQIRLSEPVIAFATSSTASKLPKLPLGTVLIDSPAFLSMMTEINRSDG--- 196
           P  + +E+S  + LS   +AFA SSTA+KLP      VL+DSP F S++ +  ++ G   
Sbjct: 136 PALTPAEVSRLVSLSGASVAFAVSSTATKLPAGLTTVVLLDSPHFRSLLMDCGQAQGQEP 195

Query: 197 LADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGETEPRRVSL 256
           L  V V QS++AAI YSSGTTGRVK   L HR+ I + +  + L ++     T      L
Sbjct: 196 LPVVVVRQSETAAIQYSSGTTGRVKAAALPHRSFIAMVAGFHALRAKAREVRT------L 255

Query: 257 CLLPLFHVFGFVMLLRAISRGETLVL----MQKFDFVEMLSAVEKFRVTYIPVSPPLVVA 316
              P+FH  GF+ +L+ ++ G T V+    + +     ++ A E++ V  +  SPP+V+ 
Sbjct: 256 LGAPMFHSMGFLFVLQGVALGATTVVVTDAVARAGIRGLVEAAERWAVMDMTASPPVVLG 315

Query: 317 MAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAARSMG 376
           M K     +  L +L+ + CGGAPL    IE+F ++ P V++  GYG TE+  G +R + 
Sbjct: 316 MTKQ----RCRLPALERITCGGAPLPAPAIERFRRRFPHVDLCMGYGSTEA-GGISRMIS 375

Query: 377 PEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKATTETL 436
            EE ++  S GR++EN+E  IVD  +G+ LP G +GELW+RGP +M GYVGD +A   T 
Sbjct: 376 QEECNHIGSAGRVTENVEVKIVDHVTGKPLPAGQQGELWVRGPAVMTGYVGDNEANATTF 435

Query: 437 HPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAVIPY 496
           + +GWLKTGDLCY D DGFL++VDRLKELIKYKAYQVPPAELE +L S P+I+DAAV+PY
Sbjct: 436 NSEGWLKTGDLCYIDQDGFLFVVDRLKELIKYKAYQVPPAELELVLHSLPQIVDAAVMPY 495

Query: 497 PDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKILRR 551
           P E+AG+IP+A VV++PGS +TEA+V+  +AKQVAPYKKIR+V F+D+IPKSP+GKILRR
Sbjct: 496 PHEEAGQIPVALVVKQPGSKLTEAEVMYNVAKQVAPYKKIRKVLFVDSIPKSPSGKILRR 551

BLAST of Carg07589 vs. ExPASy Swiss-Prot
Match: M4IQR7 (Probable CoA ligase CCL5 OS=Humulus lupulus OX=3486 GN=CCL5 PE=2 SV=1)

HSP 1 Score: 463.0 bits (1190), Expect = 4.8e-129
Identity = 257/553 (46.47%), Postives = 361/553 (65.28%), Query Frame = 0

Query: 14  VDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPSNAAVLVDSNSGVH 73
           VD RSG+C    IF+S R P+ +P  +  + VT        SS         +D+ +G H
Sbjct: 9   VDERSGYCKSNSIFYSKREPVQLPQ-NHSVDVTTFI-----SSRAHHGKIAFIDAATGRH 68

Query: 74  LSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALLSLGVVISPANPIG 133
           L++    R + ++A+ L A+  +  G V  +L+P S+  PV+  A++SLG +I+  NP+ 
Sbjct: 69  LTFPQLWRAVDSVATCLSAM-GIRKGDVILLLSPNSIYFPVVCLAVMSLGAIITTTNPLN 128

Query: 134 SDSEISHQIRLSEPVIAFATSSTASKLPKLPLGTVLIDSPAFLSMMTEINRSDGLADV-- 193
           +  EI+ QI  S+PV+AF      SK+    L  V+ID     S+   +N    L ++  
Sbjct: 129 TPREIAKQITDSKPVLAFTIPQLVSKIAGSNLPIVIIDDEVKSSLEKTLNIVSSLGEMMR 188

Query: 194 ----------KVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGETE 253
                     +V+Q D+A +LYSSGTTG  KGV+ SH+NLI +  +   +LS    G  +
Sbjct: 189 KEPSPNRIGYRVNQEDTATLLYSSGTTGASKGVVSSHKNLIAMVQT---ILSRF--GTED 248

Query: 254 PRRVSLCLLPLFHVFGF-VMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPL 313
                +C +P+FH++G     +  +S G T+V++ KF+  EMLSA+EK+R TY+P+ PP+
Sbjct: 249 GEHTFICTVPMFHIYGLAAFAMGLLSSGSTIVILSKFEIHEMLSAIEKYRATYLPLVPPI 308

Query: 314 VVAMAK--SELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGA 373
           ++A+ K  + + AKYDLSSLQ +  GGAPL KEVIE F +  P V I+QGYGLTEST   
Sbjct: 309 LMALLKNANHIRAKYDLSSLQSVLSGGAPLSKEVIEGFVENYPTVSILQGYGLTESTGIG 368

Query: 374 ARSMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKA 433
           A +   +ES    + G LS +MEA IV+P++GEAL     GELWLRGP IMKGY  + +A
Sbjct: 369 ASTDCLQESRRYGTAGMLSPSMEAKIVNPETGEALSVNRTGELWLRGPTIMKGYFSNEEA 428

Query: 434 TTETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDA 493
           T+ T+  +GWL+TGDLCY D DGF+++VDRLKELIKYK YQV PAELE LL S+PEI DA
Sbjct: 429 TSSTIDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLSHPEISDA 488

Query: 494 AVIPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAG 552
           AVIPYPD++AG+ PMAYVVRK GSN++E+ V+DFIAK VAPYK+IR+V+F+ +IPK+P+G
Sbjct: 489 AVIPYPDKEAGQFPMAYVVRKGGSNLSESTVMDFIAKLVAPYKRIRKVAFVASIPKNPSG 548

BLAST of Carg07589 vs. ExPASy TrEMBL
Match: A0A6J1EA56 (4-coumarate--CoA ligase-like 9 OS=Cucurbita moschata OX=3662 GN=LOC111431280 PE=4 SV=1)

HSP 1 Score: 1076.2 bits (2782), Expect = 0.0e+00
Identity = 554/557 (99.46%), Postives = 556/557 (99.82%), Query Frame = 0

Query: 1   MADRNPSLNAAHFVDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPS 60
           MADRNPSLNAAHFVDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPS
Sbjct: 1   MADRNPSLNAAHFVDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPS 60

Query: 61  NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL 120
           NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL
Sbjct: 61  NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL 120

Query: 121 SLGVVISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLPLGTVLIDSPAFLSMMT 180
           SLGVVISPANPIGSDSEISHQIRLS+PVIAFATSSTASKLPKL LGTVLIDSPAFLSMMT
Sbjct: 121 SLGVVISPANPIGSDSEISHQIRLSKPVIAFATSSTASKLPKLQLGTVLIDSPAFLSMMT 180

Query: 181 EINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET 240
           EINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET
Sbjct: 181 EINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET 240

Query: 241 EPRRVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPL 300
           EPR+VSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPL
Sbjct: 241 EPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPL 300

Query: 301 VVAMAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR 360
           VVAMAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR
Sbjct: 301 VVAMAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR 360

Query: 361 SMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKATT 420
           SMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKATT
Sbjct: 361 SMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKATT 420

Query: 421 ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV 480
           ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV
Sbjct: 421 ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV 480

Query: 481 IPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI 540
           IPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI
Sbjct: 481 IPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI 540

Query: 541 LRRELTQHARSHGSNKL 558
           LRRELTQHARSHGSNKL
Sbjct: 541 LRRELTQHARSHGSNKL 557

BLAST of Carg07589 vs. ExPASy TrEMBL
Match: A0A6J1KJC1 (4-coumarate--CoA ligase-like 9 OS=Cucurbita maxima OX=3661 GN=LOC111495139 PE=4 SV=1)

HSP 1 Score: 1063.1 bits (2748), Expect = 3.9e-307
Identity = 548/557 (98.38%), Postives = 552/557 (99.10%), Query Frame = 0

Query: 1   MADRNPSLNAAHFVDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPS 60
           MADRNPSLNAAHFVD RSGFCPQTKIF SLRPPLLIPPLSQPLSVTEHALSLLQSSPPPS
Sbjct: 1   MADRNPSLNAAHFVDLRSGFCPQTKIFRSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPS 60

Query: 61  NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL 120
           NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL
Sbjct: 61  NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL 120

Query: 121 SLGVVISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLPLGTVLIDSPAFLSMMT 180
           SLGV+ISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKL LGTVLIDSPAFLSMMT
Sbjct: 121 SLGVIISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLQLGTVLIDSPAFLSMMT 180

Query: 181 EINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET 240
           EINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET
Sbjct: 181 EINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET 240

Query: 241 EPRRVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPL 300
           EPR+VSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPL
Sbjct: 241 EPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPL 300

Query: 301 VVAMAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR 360
           VVA+AKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR
Sbjct: 301 VVALAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR 360

Query: 361 SMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKATT 420
           SMGPEESSNTKSVGRLSENMEAMIVDP SGEALPPGHRGELWLRGPGIMKGYVGDA ATT
Sbjct: 361 SMGPEESSNTKSVGRLSENMEAMIVDPASGEALPPGHRGELWLRGPGIMKGYVGDADATT 420

Query: 421 ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV 480
           ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV
Sbjct: 421 ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV 480

Query: 481 IPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI 540
           IPYPDEDAGEIPMAYVVRKPGSN+TEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI
Sbjct: 481 IPYPDEDAGEIPMAYVVRKPGSNVTEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI 540

Query: 541 LRRELTQHARSHGSNKL 558
           LRRELTQHARSHGSNKL
Sbjct: 541 LRRELTQHARSHGSNKL 557

BLAST of Carg07589 vs. ExPASy TrEMBL
Match: A0A1S4DV63 (4-coumarate--CoA ligase-like 9 OS=Cucumis melo OX=3656 GN=LOC103489008 PE=4 SV=1)

HSP 1 Score: 912.5 bits (2357), Expect = 8.4e-262
Identity = 469/558 (84.05%), Postives = 505/558 (90.50%), Query Frame = 0

Query: 1   MADRNPSLNAA-HFVDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPP 60
           MA RN + NAA H +D RSGFCP TKIFHSLRPPL +PPLSQPLSVT+HA SLLQSS PP
Sbjct: 1   MAARNSNFNAAPHSIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSSSPP 60

Query: 61  SNAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFAL 120
            N+ +L+DSNSG+HLSYAIFLRQIR LASNLK+LTSL NG VAFILAPTSL++PVLYFAL
Sbjct: 61  PNSTLLIDSNSGLHLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFAL 120

Query: 121 LSLGVVISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLPLGTVLIDSPAFLSMM 180
           LSLGVV+SPANP  S SEISHQI+LS+PVIAFATSSTASKLP L  GTV+IDSP FLSM+
Sbjct: 121 LSLGVVLSPANPTSSVSEISHQIQLSKPVIAFATSSTASKLPTLRFGTVIIDSPHFLSML 180

Query: 181 TEINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGE 240
           TE N SDGL D+K+DQSDSAAILYSSGTTGRVKGVLLSHRNLI VNS P    SE++ GE
Sbjct: 181 TETNGSDGLTDIKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTAFQSEIHEGE 240

Query: 241 TEPRRVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPP 300
            +P  V+LCLLPLFHVFGFVML+RAISRGETLVLM++F+F  ML AVEKFRV YIPVSPP
Sbjct: 241 MKPHPVALCLLPLFHVFGFVMLVRAISRGETLVLMERFEFEGMLRAVEKFRVIYIPVSPP 300

Query: 301 LVVAMAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAA 360
           LVVAMAKS+LVAKYDLSSLQ+LGCGGAPLGKEVI+KFHQKLP VEI QGYGLTESTAGAA
Sbjct: 301 LVVAMAKSDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGLTESTAGAA 360

Query: 361 RSMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKAT 420
           R+M PEE SNTKSVGRLS +MEA IVDP SGEAL P H+GELWLRGP IMKGYVGD KAT
Sbjct: 361 RTMEPEEISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKGYVGDDKAT 420

Query: 421 TETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA 480
           TETL PDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA
Sbjct: 421 TETLDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA 480

Query: 481 VIPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGK 540
           VIPYPDE+AGEIPMAYVVRKPGSNI+EAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGK
Sbjct: 481 VIPYPDEEAGEIPMAYVVRKPGSNISEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGK 540

Query: 541 ILRRELTQHARSHGSNKL 558
           ILRREL +HA SHGS+KL
Sbjct: 541 ILRRELAKHALSHGSSKL 558

BLAST of Carg07589 vs. ExPASy TrEMBL
Match: A0A5D3BKJ5 (4-coumarate-CoA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004520 PE=4 SV=1)

HSP 1 Score: 904.0 bits (2335), Expect = 3.0e-259
Identity = 469/569 (82.43%), Postives = 505/569 (88.75%), Query Frame = 0

Query: 1   MADRNPSLNAA-HFVDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPP 60
           MA RN + NAA H +D RSGFCP TKIFHSLRPPL +PPLSQPLSVT+HA SLLQSS PP
Sbjct: 1   MAARNSNFNAAPHSIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSSSPP 60

Query: 61  SNAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFAL 120
            N+ +L+DSNSG+HLSYAIFLRQIR LASNLK+LTSL NG VAFILAPTSL++PVLYFAL
Sbjct: 61  PNSTLLIDSNSGLHLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFAL 120

Query: 121 LSLGVVISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLPLGTVLIDSPAFLSMM 180
           LSLGVV+SPANP  S SEISHQI+LS+PVIAFATSSTASKLP L  GTV+IDSP FLSM+
Sbjct: 121 LSLGVVLSPANPTSSVSEISHQIQLSKPVIAFATSSTASKLPTLRFGTVIIDSPHFLSML 180

Query: 181 TEINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGE 240
           TE N SDGL D+K+DQSDSAAILYSSGTTGRVKGVLLSHRNLI VNS P    SE++ GE
Sbjct: 181 TETNGSDGLTDIKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTAFQSEIHEGE 240

Query: 241 TEPRRVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPP 300
            +P  V+LCLLPLFHVFGFVML+RAISRGETLVLM++F+F  ML AVEKFRV YIPVSPP
Sbjct: 241 MKPHPVALCLLPLFHVFGFVMLVRAISRGETLVLMERFEFEGMLRAVEKFRVIYIPVSPP 300

Query: 301 LVVAMAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAA 360
           LVVAMAKS+LVAKYDLSSLQ+LGCGGAPLGKEVI+KFHQKLP VEI QGYGLTESTAGAA
Sbjct: 301 LVVAMAKSDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGLTESTAGAA 360

Query: 361 RSMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKAT 420
           R+M PEE SNTKSVGRLS +MEA IVDP SGEAL P H+GELWLRGP IMKGYVGD KAT
Sbjct: 361 RTMEPEEISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKGYVGDDKAT 420

Query: 421 TETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA 480
           TETL PDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA
Sbjct: 421 TETLDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA 480

Query: 481 VIPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQ-----------VAPYKKIRRVSF 540
           VIPYPDE+AGEIPMAYVVRKPGSNI+EAQVIDFIAKQ           VAPYKKIRRVSF
Sbjct: 481 VIPYPDEEAGEIPMAYVVRKPGSNISEAQVIDFIAKQAMLHLSRNFQIVAPYKKIRRVSF 540

Query: 541 IDAIPKSPAGKILRRELTQHARSHGSNKL 558
           IDAIPKSPAGKILRREL +HA SHGS+KL
Sbjct: 541 IDAIPKSPAGKILRRELAKHALSHGSSKL 569

BLAST of Carg07589 vs. ExPASy TrEMBL
Match: E5GBV5 (4-coumarate-CoA ligase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 904.0 bits (2335), Expect = 3.0e-259
Identity = 469/569 (82.43%), Postives = 505/569 (88.75%), Query Frame = 0

Query: 1    MADRNPSLNAA-HFVDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPP 60
            MA RN + NAA H +D RSGFCP TKIFHSLRPPL +PPLSQPLSVT+HA SLLQSS PP
Sbjct: 487  MAARNSNFNAAPHSIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSSSPP 546

Query: 61   SNAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFAL 120
             N+ +L+DSNSG+HLSYAIFLRQIR LASNLK+LTSL NG VAFILAPTSL++PVLYFAL
Sbjct: 547  PNSTLLIDSNSGLHLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFAL 606

Query: 121  LSLGVVISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLPLGTVLIDSPAFLSMM 180
            LSLGVV+SPANP  S SEISHQI+LS+PVIAFATSSTASKLP L  GTV+IDSP FLSM+
Sbjct: 607  LSLGVVLSPANPTSSVSEISHQIQLSKPVIAFATSSTASKLPTLRFGTVIIDSPHFLSML 666

Query: 181  TEINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGE 240
            TE N SDGL D+K+DQSDSAAILYSSGTTGRVKGVLLSHRNLI VNS P    SE++ GE
Sbjct: 667  TETNGSDGLTDIKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTAFQSEIHEGE 726

Query: 241  TEPRRVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPP 300
             +P  V+LCLLPLFHVFGFVML+RAISRGETLVLM++F+F  ML AVEKFRV YIPVSPP
Sbjct: 727  MKPHPVALCLLPLFHVFGFVMLVRAISRGETLVLMERFEFEGMLRAVEKFRVIYIPVSPP 786

Query: 301  LVVAMAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAA 360
            LVVAMAKS+LVAKYDLSSLQ+LGCGGAPLGKEVI+KFHQKLP VEI QGYGLTESTAGAA
Sbjct: 787  LVVAMAKSDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGLTESTAGAA 846

Query: 361  RSMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKAT 420
            R+M PEE SNTKSVGRLS +MEA IVDP SGEAL P H+GELWLRGP IMKGYVGD KAT
Sbjct: 847  RTMEPEEISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKGYVGDDKAT 906

Query: 421  TETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA 480
            TETL PDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA
Sbjct: 907  TETLDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA 966

Query: 481  VIPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQ-----------VAPYKKIRRVSF 540
            VIPYPDE+AGEIPMAYVVRKPGSNI+EAQVIDFIAKQ           VAPYKKIRRVSF
Sbjct: 967  VIPYPDEEAGEIPMAYVVRKPGSNISEAQVIDFIAKQAMLHLSRNFQIVAPYKKIRRVSF 1026

Query: 541  IDAIPKSPAGKILRRELTQHARSHGSNKL 558
            IDAIPKSPAGKILRREL +HA SHGS+KL
Sbjct: 1027 IDAIPKSPAGKILRRELAKHALSHGSSKL 1055

BLAST of Carg07589 vs. TAIR 10
Match: AT5G63380.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 669.5 bits (1726), Expect = 2.4e-192
Identity = 356/565 (63.01%), Postives = 436/565 (77.17%), Query Frame = 0

Query: 9   NAAHFVDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPSNA------ 68
           N +  +D  SGF  +T I+HSLRP L +PP+ QPLS  E ALSLL  S PP+ A      
Sbjct: 6   NDSRLIDRSSGFDQRTGIYHSLRPSLSLPPIDQPLSAAEFALSLLLKSSPPATAGKNIEA 65

Query: 69  -AVLVDSNSGVHLSYAIFLRQIRTLASNLK-ALTSLSNGQVAFILAPTSLEVPVLYFALL 128
              LV+S+SG +L+Y   LR++R+LA +L+    SL++  VAFIL+P+SL++PVLY AL+
Sbjct: 66  LTYLVNSSSGDNLTYGELLRRVRSLAVSLRERFPSLASRNVAFILSPSSLDIPVLYLALM 125

Query: 129 SLGVVISPANPIGSDSEISHQIRLSEPVIAFATSSTASKL--PKLPLGTVLIDSPAFLSM 188
           S+GVV+SPANPIGS+SE+SHQ+ +SEPVIAFATS T  KL    LPLGTVL+DS  FLS 
Sbjct: 126 SIGVVVSPANPIGSESEVSHQVEVSEPVIAFATSQTVKKLQSSSLPLGTVLMDSTEFLSW 185

Query: 189 MTEINRSDGLA----DVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVN--SSPNPLL 248
              +NRSD  +     V+V+QSD AAIL+SSGTTGRVKGVLL+HRNLI     S    L 
Sbjct: 186 ---LNRSDSSSVNPFQVQVNQSDPAAILFSSGTTGRVKGVLLTHRNLIASTAVSHQRTLQ 245

Query: 249 SEVNNGETEPRRVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVT 308
             VN       RV L  LPLFHVFGF+M++RAIS GETLVL+ +F+   M  AVEK++VT
Sbjct: 246 DPVNYD-----RVGLFSLPLFHVFGFMMMIRAISLGETLVLLGRFELEAMFKAVEKYKVT 305

Query: 309 YIPVSPPLVVAMAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLT 368
            +PVSPPL+VA+ KSEL  KYDL SL+ LGCGGAPLGK++ E+F QK P V+I+QGYGLT
Sbjct: 306 GMPVSPPLIVALVKSELTKKYDLRSLRSLGCGGAPLGKDIAERFKQKFPDVDIVQGYGLT 365

Query: 369 ESTAGAARSMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGY 428
           ES+  AA + GPEE     SVGR+SENMEA IVDP +GE+LPPG  GELWLRGP IMKGY
Sbjct: 366 ESSGPAASTFGPEEMVKYGSVGRISENMEAKIVDPSTGESLPPGKTGELWLRGPVIMKGY 425

Query: 429 VGDAKATTETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSN 488
           VG+ KA+ ET+  +GWLKTGDLCYFDS+ FLYIVDRLKELIKYKAYQVPP ELE +L SN
Sbjct: 426 VGNEKASAETVDKEGWLKTGDLCYFDSEDFLYIVDRLKELIKYKAYQVPPVELEQILHSN 485

Query: 489 PEIIDAAVIPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAI 548
           P++IDAAV+P+PDEDAGEIPMA++VRKPGSN+ EAQ+IDF+AKQV PYKK+RRV+FI+AI
Sbjct: 486 PDVIDAAVVPFPDEDAGEIPMAFIVRKPGSNLNEAQIIDFVAKQVTPYKKVRRVAFINAI 545

Query: 549 PKSPAGKILRRELTQHARSHGSNKL 558
           PK+PAGKILRRELT+ A    ++KL
Sbjct: 546 PKNPAGKILRRELTKIAVDGNASKL 562

BLAST of Carg07589 vs. TAIR 10
Match: AT1G20510.1 (OPC-8:0 CoA ligase1 )

HSP 1 Score: 454.9 bits (1169), Expect = 9.3e-128
Identity = 245/552 (44.38%), Postives = 362/552 (65.58%), Query Frame = 0

Query: 14  VDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPSNAAVLVDSNSGVH 73
           V+ RSGFC     F+S R P+ +PP +  L VT        SS         +D+++G +
Sbjct: 4   VNSRSGFCNSNSTFYSKRTPIPLPP-NPSLDVTTFI-----SSQAHRGRIAFIDASTGQN 63

Query: 74  LSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALLSLGVVISPANPIG 133
           L++    R + ++A  L  +  +  G V  +L+P S+  PV+  +++SLG +I+  NP+ 
Sbjct: 64  LTFTELWRAVESVADCLSEI-GIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLN 123

Query: 134 SDSEISHQIRLSEPVIAFAT-------SSTASKLPKLPLGTVLIDSPAFLSMMTEINR-- 193
           + +EI+ QI+ S PV+AF T       S+ A KLP + +    +DS   +  + E+ +  
Sbjct: 124 TSNEIAKQIKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEERVDSVGDVRRLVEMMKKE 183

Query: 194 -SDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGETEPR 253
            S      +VDQ D+A +LYSSGTTG  KGV+ SHRNLI +  +   +++   + + E R
Sbjct: 184 PSGNRVKERVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQT---IVNRFGSDDGEQR 243

Query: 254 RVSLCLLPLFHVFGFVMLLRA-ISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPLVV 313
              +C +P+FH++G        ++ G T++++ KF+  EM+SA+ K++ T +P+ PP++V
Sbjct: 244 --FICTVPMFHIYGLAAFATGLLAYGSTIIVLSKFEMHEMMSAIGKYQATSLPLVPPILV 303

Query: 314 AMAK--SELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR 373
           AM     ++ AKYDLSS+  + CGGAPL KEV E F +K P V+I+QGYGLTEST   A 
Sbjct: 304 AMVNGADQIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTGIGAS 363

Query: 374 SMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKATT 433
           +   EES    + G+LS +ME  IVDP +G+ L P   GELWL+GP IMKGY  + +AT+
Sbjct: 364 TDTVEESRRYGTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYFSNEEATS 423

Query: 434 ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV 493
            TL  +GWL+TGDLCY D DGF+++VDRLKELIKYK YQV PAELE LL ++PEI DAAV
Sbjct: 424 STLDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEITDAAV 483

Query: 494 IPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI 553
           IP+PD++ G+ PMAYVVRK GS+++E  +++F+AKQVAPYK+IR+V+F+ +IPK+P+GKI
Sbjct: 484 IPFPDKEVGQFPMAYVVRKTGSSLSEKTIMEFVAKQVAPYKRIRKVAFVSSIPKNPSGKI 543

BLAST of Carg07589 vs. TAIR 10
Match: AT1G20480.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 453.8 bits (1166), Expect = 2.1e-127
Identity = 261/554 (47.11%), Postives = 351/554 (63.36%), Query Frame = 0

Query: 14  VDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPSNAAVLVDSNSGVH 73
           VD +SGFC  T IF+S R P+ +PP +Q L VT        +S P     V VD+ +G  
Sbjct: 21  VDRKSGFCESTSIFYSKREPMALPP-NQFLDVTSFI-----ASQPHRGKTVFVDAVTGRR 80

Query: 74  LSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALLSLGVVISPANPIG 133
           LS+      +  +A  L AL  +  G V  IL+P S+  P++  +++SLG +I+ ANPI 
Sbjct: 81  LSFPELWLGVERVAGCLYAL-GVRKGNVVIILSPNSILFPIVSLSVMSLGAIITTANPIN 140

Query: 134 SDSEISHQIRLSEPVIAFATSSTASKL---PKLPLGTVLID-----SPAF---------L 193
           +  EIS QI  S PV+AF T    SKL       L  VL+D     S ++         L
Sbjct: 141 TSDEISKQIGDSRPVLAFTTCKLVSKLAAASNFNLPVVLMDDYHVPSQSYGDRVKLVGRL 200

Query: 194 SMMTEINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVN 253
             M E   S+     +V+Q D+AA+LYSSGTTG  KGV+LSHRNLI         L +  
Sbjct: 201 ETMIETEPSESRVKQRVNQDDTAALLYSSGTTGTSKGVMLSHRNLIA--------LVQAY 260

Query: 254 NGETEPRRVSLCLLPLFHVFGF-VMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIP 313
                  + ++C +P+ H+FGF       I+ G T+V++ KFD  ++LSAVE  R +Y+ 
Sbjct: 261 RARFGLEQRTICTIPMCHIFGFGGFATGLIALGWTIVVLPKFDMAKLLSAVETHRSSYLS 320

Query: 314 VSPPLVVAMAK--SELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTE 373
           + PP+VVAM    +E+ +KYDLSSL  +  GGAPL +EV EKF +  PKV+I+QGYGLTE
Sbjct: 321 LVPPIVVAMVNGANEINSKYDLSSLHTVVAGGAPLSREVTEKFVENYPKVKILQGYGLTE 380

Query: 374 STAGAARSMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYV 433
           STA AA     EE+    + G L+ N+E  IVDPD+G  L     GELW+R P +MKGY 
Sbjct: 381 STAIAASMFNKEETKRYGASGLLAPNVEGKIVDPDTGRVLGVNQTGELWIRSPTVMKGYF 440

Query: 434 GDAKATTETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNP 493
            + +AT  T+  +GWLKTGDLCY D DGF+++VDRLKELIK   YQV PAELE LL ++P
Sbjct: 441 KNKEATASTIDSEGWLKTGDLCYIDGDGFVFVVDRLKELIKCNGYQVAPAELEALLLAHP 500

Query: 494 EIIDAAVIPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAIP 548
           EI DAAVIP PD  AG+ PMAY+VRK GSN++E++++ F+AKQV+PYKKIR+V+F+ +IP
Sbjct: 501 EIADAAVIPIPDMKAGQYPMAYIVRKVGSNLSESEIMGFVAKQVSPYKKIRKVTFLASIP 559

BLAST of Carg07589 vs. TAIR 10
Match: AT1G20500.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 442.6 bits (1137), Expect = 4.8e-124
Identity = 249/554 (44.95%), Postives = 349/554 (63.00%), Query Frame = 0

Query: 14  VDHRSGFCPQTKIFHSLRPPLLIPP-LSQPLSVTEHALSLLQSSPPPSNAAVLVDSNSGV 73
           VD RSGFC     F+S R PL +PP LS+ ++          SS P       +D+ +G 
Sbjct: 10  VDPRSGFCKSNSTFYSKRQPLSLPPNLSRDVTT-------FISSQPHRGKTAFIDAATGQ 69

Query: 74  HLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALLSLGVVISPANPI 133
            L+++   R +  +A  L     +  G V  IL+P S+ +PV+  +++SLG V + AN +
Sbjct: 70  CLTFSDLWRAVDRVADCLYHEVGIRRGDVVLILSPNSIFIPVVCLSVMSLGAVFTTANTL 129

Query: 134 GSDSEISHQIRLSEPVIAFATSSTASKLPKLPLGTVLIDSPAF------------LSMMT 193
            +  EIS QI  S P + F T   A KLP + +  VL D   +            LS M 
Sbjct: 130 NTSGEISKQIADSNPTLVFTTRQLAPKLP-VAISVVLTDDEVYQELTSAIRVVGILSEMV 189

Query: 194 EINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET 253
           +   S      +V+Q D+A +LYSSGTTG  KGV+ SHRNL          ++   +   
Sbjct: 190 KKEPSGQRVRDRVNQDDTAMMLYSSGTTGPSKGVISSHRNL-------TAHVARFISDNL 249

Query: 254 EPRRVSLCLLPLFHVFGFV-MLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPP 313
           +   + +C +P+FH +G +   +  ++ G T+V++++F   +M+ AVEK R T + ++PP
Sbjct: 250 KRDDIFICTVPMFHTYGLLTFAMGTVALGSTVVILRRFQLHDMMDAVEKHRATALALAPP 309

Query: 314 LVVAMAKSE--LVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAG 373
           ++VAM      + AKYDLSSL+ + CGGAPL KEV E F +K P V+I+QGY LTES  G
Sbjct: 310 VLVAMINDADLIKAKYDLSSLKTVRCGGAPLSKEVTEGFLEKYPTVDILQGYALTESNGG 369

Query: 374 AARSMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAK 433
            A +   EES    + G L+ ++EA IVDP++G  +     GELWL+GP I KGY  + +
Sbjct: 370 GAFTNSAEESRRYGTAGTLTSDVEARIVDPNTGRFMGINQTGELWLKGPSISKGYFKNQE 429

Query: 434 ATTETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIID 493
           AT ET++ +GWLKTGDLCY D DGFL++VDRLKELIKYK YQVPPAELE LL ++P+I+D
Sbjct: 430 ATNETINLEGWLKTGDLCYIDEDGFLFVVDRLKELIKYKGYQVPPAELEALLITHPDILD 489

Query: 494 AAVIPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPA 552
           AAVIP+PD++AG+ PMAYVVRK  SN++E QVIDFI+KQVAPYKKIR+VSFI++IPK+ +
Sbjct: 490 AAVIPFPDKEAGQYPMAYVVRKHESNLSEKQVIDFISKQVAPYKKIRKVSFINSIPKTAS 548

BLAST of Carg07589 vs. TAIR 10
Match: AT5G38120.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 423.3 bits (1087), Expect = 3.0e-118
Identity = 239/560 (42.68%), Postives = 347/560 (61.96%), Query Frame = 0

Query: 7   SLNAAHFVDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPSNAAVLV 66
           S  ++  +D R+GFC     F+S R PL +P   + L +T        SS         +
Sbjct: 4   SQRSSSLIDPRNGFCTSNSTFYSKRKPLALPS-KESLDITTFI-----SSQTYRGKTAFI 63

Query: 67  DSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALLSLGVVI 126
           D+ +   +S++     +  +A  L     +  G V  +L+P ++ +P++  +++SLG V+
Sbjct: 64  DAATDHRISFSDLWMAVDRVADCLLHDVGIRRGDVVLVLSPNTISIPIVCLSVMSLGAVL 123

Query: 127 SPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLPLGTVL------IDSPAFLSM-- 186
           + ANP+ + SEI  QI  S P +AF T   A K+    +  VL      +  P  L +  
Sbjct: 124 TTANPLNTASEILRQIADSNPKLAFTTPELAPKIASSGISIVLERVEDTLRVPRGLKVVG 183

Query: 187 -MTEINRSDGLADV---KVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSE 246
            +TE+ + +        +V + D+A +LYSSGTTGR KGV  SH NLI        +   
Sbjct: 184 NLTEMMKKEPSGQAVRNQVHKDDTAMLLYSSGTTGRSKGVNSSHGNLIA------HVARY 243

Query: 247 VNNGETEPRRVSLCLLPLFHVFGFV-MLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTY 306
           +     +P++  +C +PLFH FG +  +L  ++ G T+V++ +FD  EM++AVEK+R T 
Sbjct: 244 IAEPFEQPQQTFICTVPLFHTFGLLNFVLATLALGTTVVILPRFDLGEMMAAVEKYRATT 303

Query: 307 IPVSPPLVVAMAK--SELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGL 366
           + + PP++V M     +++ KYD+S L+ + CGGAPL KEV + F +K P V++ QGY L
Sbjct: 304 LILVPPVLVTMINKADQIMKKYDVSFLRTVRCGGAPLSKEVTQGFMKKYPTVDVYQGYAL 363

Query: 367 TESTAGAARSMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKG 426
           TES    A     EES    +VG LS  +EA IVDP++G+ +     GELWL+GP I KG
Sbjct: 364 TESNGAGASIESVEESRRYGAVGLLSCGVEARIVDPNTGQVMGLNQTGELWLKGPSIAKG 423

Query: 427 YVGDAKATTETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQS 486
           Y  + +   E +  +GWLKTGDLCY D+DGFL+IVDRLKELIKYK YQVPPAELE LL +
Sbjct: 424 YFRNEE---EIITSEGWLKTGDLCYIDNDGFLFIVDRLKELIKYKGYQVPPAELEALLLN 483

Query: 487 NPEIIDAAVIPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDA 546
           +P+I+DAAVIP+PD++AG+ PMAYV RKP SN+ E +VIDFI+KQVAPYKKIR+V+FID+
Sbjct: 484 HPDILDAAVIPFPDKEAGQFPMAYVARKPESNLCEKKVIDFISKQVAPYKKIRKVAFIDS 543

Query: 547 IPKSPAGKILRRELTQHARS 552
           IPK+P+GK LR++L + A S
Sbjct: 544 IPKTPSGKTLRKDLIKFAIS 548