Carg07500 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg07500
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionElongator complex protein 5
LocationCarg_Chr01: 2121760 .. 2126154 (-)
RNA-Seq ExpressionCarg07500
SyntenyCarg07500
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TAAACTCTAAACCTAACGCGCCTTTGTCTGTCTCTCACTTTCAGTTCGTATCCCTCTCCCCTGAGTTCTTCGTAGGTTCTTTCAATAATCTCGTACACTTCTGAATTGCTTCGTTGTTTGTCCACAATCTACTTTCAGCTTATGGTTTCTCAGTTTTCAAATTGAGTGTCGAAGTTATTCCGTAATTAAGGACTTGAAGCTGCTATATCCTCGCCAATAATGGCGGATTCTATTTGCAGAGCACTTAGAGACGGTGCGTTAGAGGGAGAGCTCGCGCCTGCTCTCACCTTAAAGGACTCCATCAATTCGCCATTCGGTTTTCACGCATTTGCCCACGTTGTGATGCAGCTTTCGAGCAATATTTTGGCGGGTAAATCGCAGTCTCGGTTAGTTTAATTCCCTTTTAGATATGTCATTTAGGAGATTGAAATTGTCCGGCTTTGGCAGTCGTTTTGACTCTTTCTTTGGTGGTGTAATTTTGATCTCAGAGGTCTCGTTTTGCTTGCGTTCTCTCGAAGTCCGGCGTATTACCTCCAGCTGTTGAAGAAGAGTGGAGTGAACGTGGGATCGTCTGAAAAATGGTGAAGTTCTCTTTGCAAATTCTTGCATCTGTGCCTTTTTTAATAGTTCAATTTCTGTGAGAAGGCCGGGATTACTAGGGAAGAAACGCTTGTTAATAGCATTAGGCGATACTCAGCTAGTAGATTATCACCATGAGGGATGTAATGAGTCTTGGTATTCTTCACATTGACTTGAATTTATCATTAGATAAAAGGCATACTCTTTCATATTTCGTATCAATGATTTCTTTTTTCCTTTGCTGTTATTTTTTATGTGCAAGTTCAATTGACTGTTCTTGTGTTATAGGATTCAGATTTTGGACTGTTATACAGATCCTCTTGGCTGGAAGGAACGGTTTACAGGGGGTGAAAAATTTTCAAACGTTCACCAAGAAGTTTCAAATTTATCTCATCTTTGTACAAATGTGAGGGATATGGATAATCTATTCTCTTCGATTATTACGCTTGGAAAAGGTATGACTATAATCTTGATTGAGAATATAGCGTAGTGATTTTCTGGGCTTATTTTGCAAGAAAAAGGTCACAAGTTAAACTCTAATGTTCTAGCAGGATTTGTTGGAGAAGGAACTGTACGCTTTTGTGTTGCCATAGACTCGGTACGTTCTCTCCCTCTTTGGTACCATTCAAGGAACCACTTATTGTTAGTATTAACAATGAGCAGACTTGAGACCCACAGTCTCTTATATTCTTTTCTTGTGTATAGTACCTATTGAATATAATTAAGAATACCAAACTGTCCAGGAGTATTCTCCTTTATCAAACCATCCAGATAATTTATTTTCTTCTGCATATTACTCATTAAATATAGTTAACATCTTGAGACTATCAAATTGTCAAAGAGCATTCTCCTTTCTGGGTTCTATGTTGAAAGTTGATACTTCCTTTCTTTGTTGAGGTGTGGCAGCGAGAAATGCGTATAAACAATGTGAATCATTAGTTGCATCTTTTTGTTTTTAGTTTGGTGCTGGTCAGATTCCATAGGGATAAGTTCCACTCTTGTTTATAGTTGTTCTGTAATGGCCCAAGCCTACCGCTAGGAGATATTTTTCTTTTTGGGCTTTCCTTTCGAGCTTCCCCTCAAGGTTTTTTAAAACGCATCTGCTAGGGAGAGGTTTTCACACCCTTATAGATGCTTCGTTTTCCTCCCTAACCGATGTAGGATCTCACAATCCGCCCCCCTTTGGGGCCCAGCTCCTTACTGGCACACTGCCTCGTGTAAACCCCCTTCAGAGCTTAGCCTCCTAGCTAGCACATCGCTCGATGTCTGGTTCTGATACTATTTGTAATGATCCAAGCCCATTGCTAGCAGATATTGTCCTCTTTGGGTTTTCCCTTTCGAGCTTCCCCTCAAGGTTTTTTTGAAAATGCGGATGCTAGGGAGAGGTTTCCACACCCTTATAAAGAATGTCTTGTTCTCCTACCCAACCGACGTGGGATCTCACATATTTCTTTGTAGATTTTTGCTCTTTTCTTTATTTCCATTCACTATTAATTTGGAAGTCGTTGCTTTTATTTTCTCATTTTTATTTAATCTTCTGAAATTTTACCAGGTAACCGATATGTTAAGGCATTCTTCTACATCAGCAGTTGCAGGTCTTTTAAGCAACCTTCGGAGCAATGGTATGGATATGATATGATATTAATAACCTCTGAAATTTTCGCTGTTTACATAAATCAAATCTCATGAGTTATGATTAATAGTTCTTCACTTGATAGCTCTTATTTAATGGTATGGATATGTTATGATATATTAATCCTGTTTGAACTGTTTACCTGTGATTTTAGATAAGGTTTCAAGTACATTTTGGTTGGTACATGAAGACCTTCACGAGGAAAAGGTCATAGCTGCTCTTGAATATATGTCTTCAATGGTGGCCACAGTTGAGCCATTAACTCCATCCCCATATGTGCGCGAAAGCAATCTGAACACTCCCTATCTTGAACACAGCTCTACTAAGGGGAGGTTTCATGTGCGGATTAAACGCAGAAATGGACGCGTGCGAGTCATTGTAAAATCTCTTCTTTTGCAAAACTTTTGGTATTTACTTTTGAAATTCACGGTTATAACCATAGATTCTTGATGTTCAATGTTTAGTGTGAAAATTTTAAAGTTGAGCAGTCGGGCATCAAATTTACGTCCATTTCATCTGAAGATGCAGTCGTCAATCAAGGGCTTGTACCAAAGGTTAGTTATCTTGATTTTGACCTCTTTCATTGTTTCCCAATATTGAATTCTTAAACTTTTGGGTTTACCAAAATTGAATTTTATGGTACAAGGAATCATTTTCTTCTTAGGTTTAGGGTTTGTTTGGAATAACTTCTAGTTTAAGCATTTTTTAGAAACGTATTTTTATATCTTACATTTTTTTTTTAATGAAAATATGTTTTTTTTTTCTTATGGTATATTTTGAAGATTTTCGGTTAATCACTTTAAAATCATTTTTGGATAATCTATGTTTAAATGTTTCTTCAAAATAGATTTTTAATTAATCTAATTTCTTAAATGCTTCTTCTTTATTAAAATTTTTCTAAAAAATACTTTTGATTTTCTAAAATCATTCAAAAAGTTACACTAATCATACTTTAAATTAGTAGTTTTTGCAATGATGCTAATAGAAAAATTGATTTTTTTTTTAAAAAAAACTTATTTTTTTTCCAAAGTTATTCCAAATGTACTTTGCATCCAAGTTTGAATGCACAAACATGAGGATCTACTCTTGATATGCCTAATCTTTCCCCAACTAGTACAGTGATAGACCCTGAGTCACTTCGGTCAATACACGTAGGATTAAGAAATAGAAAATAGGGGAGACGAATGATGTTGTATGTGAGAAGTGAGCGCGGGCGTGTTGCTGTGATTTTATGCGCCCATGTGCTCCTGTTAGTATGTTGCTGAATGTTTTGTTGTACAAGGATAGTCTTTTAAGGGCATTCCAGAGGGAAGGCGAGTGTATACTTTTGGTATTTTGTTTTAGGACTCGTAGTTTGAGTGTTCTTGAGTTAGGGGAGCCCTCGAATTGAATCTCCCTTGGCTGTTGCCATAAATCATTAGTCATTTGAACTTTTTTCTCATAAATTTTCATGAATTAAAAACTGATGTATGGACTACATTTCTTCTCTCAGGTGCAGTTCAATCTTCAGTTGTCTGAGAAAGAGCAAATTGACAGGGCTAGAGTTGTTCTTCCTTTTGAGCATCAAGGTTCGGATTGTCAATGTATCATTGCCTATTGCAAAATAATTCTAACCTGATTTGACCCTATTATCCAGGCAGTAAGGTTATCCTGAGTCTTGACTAACTGTTCAAGGTCGACTTTTCTTATAGGAAATGGTAAACCGATACAAATATACGATGGTCGAAGATCTCTTACCGAAAGCAAAGATGACGACAAACCTCTCTCGACCAGTGAGAAAAGCAAGGATGAAGGAGCAGGGAAGGGAGAGATCATATATTTTCGCGATTCAGAAGACGAGATGCCAGATTCTGATGAGGACCCTGATGATGATTTAGACATATAATTTTGAATGAAGGTAGAAGATTTGTATCATATCTATCTTTTTCAATATCACTTCAAATTGTTGCTCTCAGTTTTATGTGGAACAATGTGTGGAGTAGCAACCCAGACGTGACGACAAAATACGAACATTACCAGTTTGTTTCGTCTTCGGGATGATTTTTAGGACTGAATTATTCTACATAGTTTGTTAATTGTTTCCCATGAACCTCTTACAGTTCCATCTTTCCTTTTTCAAAGGCTGAAAAGGGTGTGAGTAAAAGATATTTAAAAAGAAAAGAAAGAGATAATAAAAACTAAAA

mRNA sequence

TAAACTCTAAACCTAACGCGCCTTTGTCTGTCTCTCACTTTCAGTTCGTATCCCTCTCCCCTGAGTTCTTCGTAGGTTCTTTCAATAATCTCGTACACTTCTGAATTGCTTCGTTGTTTGTCCACAATCTACTTTCAGCTTATGGTTTCTCAGTTTTCAAATTGAGTGTCGAAGTTATTCCGTAATTAAGGACTTGAAGCTGCTATATCCTCGCCAATAATGGCGGATTCTATTTGCAGAGCACTTAGAGACGGTGCGTTAGAGGGAGAGCTCGCGCCTGCTCTCACCTTAAAGGACTCCATCAATTCGCCATTCGGTTTTCACGCATTTGCCCACGTTGTGATGCAGCTTTCGAGCAATATTTTGGCGGGTAAATCGCAGTCTCGAGGTCTCGTTTTGCTTGCGTTCTCTCGAAGTCCGGCGTATTACCTCCAGCTGTTGAAGAAGAGTGGAGTGAACGTGGGATCGTCTGAAAAATGGATTCAGATTTTGGACTGTTATACAGATCCTCTTGGCTGGAAGGAACGGTTTACAGGGGGTGAAAAATTTTCAAACGTTCACCAAGAAGTTTCAAATTTATCTCATCTTTGTACAAATGTGAGGGATATGGATAATCTATTCTCTTCGATTATTACGCTTGGAAAAGGATTTGTTGGAGAAGGAACTGTACGCTTTTGTGTTGCCATAGACTCGGTAACCGATATGTTAAGGCATTCTTCTACATCAGCAGTTGCAGGTCTTTTAAGCAACCTTCGGAGCAATGATAAGGTTTCAAGTACATTTTGGTTGGTACATGAAGACCTTCACGAGGAAAAGGTCATAGCTGCTCTTGAATATATGTCTTCAATGGTGGCCACAGTTGAGCCATTAACTCCATCCCCATATGTGCGCGAAAGCAATCTGAACACTCCCTATCTTGAACACAGCTCTACTAAGGGGAGGTTTCATGTGCGGATTAAACGCAGAAATGGACGCGTGCGAGTCATTTGTGAAAATTTTAAAGTTGAGCAGTCGGGCATCAAATTTACGTCCATTTCATCTGAAGATGCAGTCGTCAATCAAGGGCTTGTACCAAAGGTGCAGTTCAATCTTCAGTTGTCTGAGAAAGAGCAAATTGACAGGGCTAGAGTTGTTCTTCCTTTTGAGCATCAAGGAAATGGTAAACCGATACAAATATACGATGGTCGAAGATCTCTTACCGAAAGCAAAGATGACGACAAACCTCTCTCGACCAGTGAGAAAAGCAAGGATGAAGGAGCAGGGAAGGGAGAGATCATATATTTTCGCGATTCAGAAGACGAGATGCCAGATTCTGATGAGGACCCTGATGATGATTTAGACATATAATTTTGAATGAAGGTAGAAGATTTGTATCATATCTATCTTTTTCAATATCACTTCAAATTGTTGCTCTCAGTTTTATGTGGAACAATGTGTGGAGTAGCAACCCAGACGTGACGACAAAATACGAACATTACCAGTTTGTTTCGTCTTCGGGATGATTTTTAGGACTGAATTATTCTACATAGTTTGTTAATTGTTTCCCATGAACCTCTTACAGTTCCATCTTTCCTTTTTCAAAGGCTGAAAAGGGTGTGAGTAAAAGATATTTAAAAAGAAAAGAAAGAGATAATAAAAACTAAAA

Coding sequence (CDS)

ATGGCGGATTCTATTTGCAGAGCACTTAGAGACGGTGCGTTAGAGGGAGAGCTCGCGCCTGCTCTCACCTTAAAGGACTCCATCAATTCGCCATTCGGTTTTCACGCATTTGCCCACGTTGTGATGCAGCTTTCGAGCAATATTTTGGCGGGTAAATCGCAGTCTCGAGGTCTCGTTTTGCTTGCGTTCTCTCGAAGTCCGGCGTATTACCTCCAGCTGTTGAAGAAGAGTGGAGTGAACGTGGGATCGTCTGAAAAATGGATTCAGATTTTGGACTGTTATACAGATCCTCTTGGCTGGAAGGAACGGTTTACAGGGGGTGAAAAATTTTCAAACGTTCACCAAGAAGTTTCAAATTTATCTCATCTTTGTACAAATGTGAGGGATATGGATAATCTATTCTCTTCGATTATTACGCTTGGAAAAGGATTTGTTGGAGAAGGAACTGTACGCTTTTGTGTTGCCATAGACTCGGTAACCGATATGTTAAGGCATTCTTCTACATCAGCAGTTGCAGGTCTTTTAAGCAACCTTCGGAGCAATGATAAGGTTTCAAGTACATTTTGGTTGGTACATGAAGACCTTCACGAGGAAAAGGTCATAGCTGCTCTTGAATATATGTCTTCAATGGTGGCCACAGTTGAGCCATTAACTCCATCCCCATATGTGCGCGAAAGCAATCTGAACACTCCCTATCTTGAACACAGCTCTACTAAGGGGAGGTTTCATGTGCGGATTAAACGCAGAAATGGACGCGTGCGAGTCATTTGTGAAAATTTTAAAGTTGAGCAGTCGGGCATCAAATTTACGTCCATTTCATCTGAAGATGCAGTCGTCAATCAAGGGCTTGTACCAAAGGTGCAGTTCAATCTTCAGTTGTCTGAGAAAGAGCAAATTGACAGGGCTAGAGTTGTTCTTCCTTTTGAGCATCAAGGAAATGGTAAACCGATACAAATATACGATGGTCGAAGATCTCTTACCGAAAGCAAAGATGACGACAAACCTCTCTCGACCAGTGAGAAAAGCAAGGATGAAGGAGCAGGGAAGGGAGAGATCATATATTTTCGCGATTCAGAAGACGAGATGCCAGATTCTGATGAGGACCCTGATGATGATTTAGACATATAA

Protein sequence

MADSICRALRDGALEGELAPALTLKDSINSPFGFHAFAHVVMQLSSNILAGKSQSRGLVLLAFSRSPAYYLQLLKKSGVNVGSSEKWIQILDCYTDPLGWKERFTGGEKFSNVHQEVSNLSHLCTNVRDMDNLFSSIITLGKGFVGEGTVRFCVAIDSVTDMLRHSSTSAVAGLLSNLRSNDKVSSTFWLVHEDLHEEKVIAALEYMSSMVATVEPLTPSPYVRESNLNTPYLEHSSTKGRFHVRIKRRNGRVRVICENFKVEQSGIKFTSISSEDAVVNQGLVPKVQFNLQLSEKEQIDRARVVLPFEHQGNGKPIQIYDGRRSLTESKDDDKPLSTSEKSKDEGAGKGEIIYFRDSEDEMPDSDEDPDDDLDI
Homology
BLAST of Carg07500 vs. NCBI nr
Match: KAG7036759.1 (Elongator complex protein 5 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 735.7 bits (1898), Expect = 2.0e-208
Identity = 375/375 (100.00%), Postives = 375/375 (100.00%), Query Frame = 0

Query: 1   MADSICRALRDGALEGELAPALTLKDSINSPFGFHAFAHVVMQLSSNILAGKSQSRGLVL 60
           MADSICRALRDGALEGELAPALTLKDSINSPFGFHAFAHVVMQLSSNILAGKSQSRGLVL
Sbjct: 1   MADSICRALRDGALEGELAPALTLKDSINSPFGFHAFAHVVMQLSSNILAGKSQSRGLVL 60

Query: 61  LAFSRSPAYYLQLLKKSGVNVGSSEKWIQILDCYTDPLGWKERFTGGEKFSNVHQEVSNL 120
           LAFSRSPAYYLQLLKKSGVNVGSSEKWIQILDCYTDPLGWKERFTGGEKFSNVHQEVSNL
Sbjct: 61  LAFSRSPAYYLQLLKKSGVNVGSSEKWIQILDCYTDPLGWKERFTGGEKFSNVHQEVSNL 120

Query: 121 SHLCTNVRDMDNLFSSIITLGKGFVGEGTVRFCVAIDSVTDMLRHSSTSAVAGLLSNLRS 180
           SHLCTNVRDMDNLFSSIITLGKGFVGEGTVRFCVAIDSVTDMLRHSSTSAVAGLLSNLRS
Sbjct: 121 SHLCTNVRDMDNLFSSIITLGKGFVGEGTVRFCVAIDSVTDMLRHSSTSAVAGLLSNLRS 180

Query: 181 NDKVSSTFWLVHEDLHEEKVIAALEYMSSMVATVEPLTPSPYVRESNLNTPYLEHSSTKG 240
           NDKVSSTFWLVHEDLHEEKVIAALEYMSSMVATVEPLTPSPYVRESNLNTPYLEHSSTKG
Sbjct: 181 NDKVSSTFWLVHEDLHEEKVIAALEYMSSMVATVEPLTPSPYVRESNLNTPYLEHSSTKG 240

Query: 241 RFHVRIKRRNGRVRVICENFKVEQSGIKFTSISSEDAVVNQGLVPKVQFNLQLSEKEQID 300
           RFHVRIKRRNGRVRVICENFKVEQSGIKFTSISSEDAVVNQGLVPKVQFNLQLSEKEQID
Sbjct: 241 RFHVRIKRRNGRVRVICENFKVEQSGIKFTSISSEDAVVNQGLVPKVQFNLQLSEKEQID 300

Query: 301 RARVVLPFEHQGNGKPIQIYDGRRSLTESKDDDKPLSTSEKSKDEGAGKGEIIYFRDSED 360
           RARVVLPFEHQGNGKPIQIYDGRRSLTESKDDDKPLSTSEKSKDEGAGKGEIIYFRDSED
Sbjct: 301 RARVVLPFEHQGNGKPIQIYDGRRSLTESKDDDKPLSTSEKSKDEGAGKGEIIYFRDSED 360

Query: 361 EMPDSDEDPDDDLDI 376
           EMPDSDEDPDDDLDI
Sbjct: 361 EMPDSDEDPDDDLDI 375

BLAST of Carg07500 vs. NCBI nr
Match: KAG6607058.1 (Elongator complex protein 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 732.3 bits (1889), Expect = 2.2e-207
Identity = 372/375 (99.20%), Postives = 375/375 (100.00%), Query Frame = 0

Query: 1   MADSICRALRDGALEGELAPALTLKDSINSPFGFHAFAHVVMQLSSNILAGKSQSRGLVL 60
           MADSICRALRDGALEGELAPALTLKDSINSPFGFHAFAHV++QLSSNILAGKSQSRGLVL
Sbjct: 1   MADSICRALRDGALEGELAPALTLKDSINSPFGFHAFAHVLLQLSSNILAGKSQSRGLVL 60

Query: 61  LAFSRSPAYYLQLLKKSGVNVGSSEKWIQILDCYTDPLGWKERFTGGEKFSNVHQEVSNL 120
           LAFSRSPAYYL+LLKKSGVNVGSSEKWIQILDCYTDPLGWKERFTGGEKFSNVHQEVSNL
Sbjct: 61  LAFSRSPAYYLELLKKSGVNVGSSEKWIQILDCYTDPLGWKERFTGGEKFSNVHQEVSNL 120

Query: 121 SHLCTNVRDMDNLFSSIITLGKGFVGEGTVRFCVAIDSVTDMLRHSSTSAVAGLLSNLRS 180
           SHLCTNVRDMDNLFSSIITLGKGFVGEGTVRFCVAIDSVTDMLRHSSTSAVAGLLSNLRS
Sbjct: 121 SHLCTNVRDMDNLFSSIITLGKGFVGEGTVRFCVAIDSVTDMLRHSSTSAVAGLLSNLRS 180

Query: 181 NDKVSSTFWLVHEDLHEEKVIAALEYMSSMVATVEPLTPSPYVRESNLNTPYLEHSSTKG 240
           NDKVSSTFWLVHEDLHEEKVIAALEYMSSMVATVEPLTPSPYVRESNLNTPYLEHSSTKG
Sbjct: 181 NDKVSSTFWLVHEDLHEEKVIAALEYMSSMVATVEPLTPSPYVRESNLNTPYLEHSSTKG 240

Query: 241 RFHVRIKRRNGRVRVICENFKVEQSGIKFTSISSEDAVVNQGLVPKVQFNLQLSEKEQID 300
           RFHVRIKRRNGRVRVICENFKVEQSGIKFTSISSEDAVVNQGLVPKVQFNLQLSEKEQID
Sbjct: 241 RFHVRIKRRNGRVRVICENFKVEQSGIKFTSISSEDAVVNQGLVPKVQFNLQLSEKEQID 300

Query: 301 RARVVLPFEHQGNGKPIQIYDGRRSLTESKDDDKPLSTSEKSKDEGAGKGEIIYFRDSED 360
           RARVVLPFEHQGNGKPIQIYDGRRSLTESKDDDKPLSTSEKSKDEGAGKGEIIYFRDSED
Sbjct: 301 RARVVLPFEHQGNGKPIQIYDGRRSLTESKDDDKPLSTSEKSKDEGAGKGEIIYFRDSED 360

Query: 361 EMPDSDEDPDDDLDI 376
           EMPDSDEDPDDDLDI
Sbjct: 361 EMPDSDEDPDDDLDI 375

BLAST of Carg07500 vs. NCBI nr
Match: XP_023521734.1 (elongator complex protein 5 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 723.8 bits (1867), Expect = 7.7e-205
Identity = 367/375 (97.87%), Postives = 372/375 (99.20%), Query Frame = 0

Query: 1   MADSICRALRDGALEGELAPALTLKDSINSPFGFHAFAHVVMQLSSNILAGKSQSRGLVL 60
           MADSICRALRDGALEGELAPALT KDSINSPFGFHAFAHV+MQLSSNILAGKSQSRGLVL
Sbjct: 1   MADSICRALRDGALEGELAPALTFKDSINSPFGFHAFAHVLMQLSSNILAGKSQSRGLVL 60

Query: 61  LAFSRSPAYYLQLLKKSGVNVGSSEKWIQILDCYTDPLGWKERFTGGEKFSNVHQEVSNL 120
           LAFSRSPAYYL+LLKKSGVNVGSSEKWIQILDCYTDPLGWKERFTGGEKFSNVHQEVSNL
Sbjct: 61  LAFSRSPAYYLELLKKSGVNVGSSEKWIQILDCYTDPLGWKERFTGGEKFSNVHQEVSNL 120

Query: 121 SHLCTNVRDMDNLFSSIITLGKGFVGEGTVRFCVAIDSVTDMLRHSSTSAVAGLLSNLRS 180
           SHLCTNVRD+DNLFSSIITLGKGFVG+G VRFCVAIDSV+DMLRHSSTSAVAGLLSNLRS
Sbjct: 121 SHLCTNVRDLDNLFSSIITLGKGFVGDGNVRFCVAIDSVSDMLRHSSTSAVAGLLSNLRS 180

Query: 181 NDKVSSTFWLVHEDLHEEKVIAALEYMSSMVATVEPLTPSPYVRESNLNTPYLEHSSTKG 240
           NDKVSSTFWLVHEDLHEEKVIAALEYMSSMVATVEPLTPSPYVRESNLNTPYLEHSSTKG
Sbjct: 181 NDKVSSTFWLVHEDLHEEKVIAALEYMSSMVATVEPLTPSPYVRESNLNTPYLEHSSTKG 240

Query: 241 RFHVRIKRRNGRVRVICENFKVEQSGIKFTSISSEDAVVNQGLVPKVQFNLQLSEKEQID 300
           RFHVRIKRRNGRVRVICENFKVEQSGI FTSISSEDAVVNQGLVPKVQFNLQLSEKEQID
Sbjct: 241 RFHVRIKRRNGRVRVICENFKVEQSGITFTSISSEDAVVNQGLVPKVQFNLQLSEKEQID 300

Query: 301 RARVVLPFEHQGNGKPIQIYDGRRSLTESKDDDKPLSTSEKSKDEGAGKGEIIYFRDSED 360
           RARVVLPFEHQGNGKPIQIYDGRRSLTESKDDDKPLSTSEKSKDEGAGKGEIIYFRDSED
Sbjct: 301 RARVVLPFEHQGNGKPIQIYDGRRSLTESKDDDKPLSTSEKSKDEGAGKGEIIYFRDSED 360

Query: 361 EMPDSDEDPDDDLDI 376
           EMPDSDEDPDDDLDI
Sbjct: 361 EMPDSDEDPDDDLDI 375

BLAST of Carg07500 vs. NCBI nr
Match: XP_022948561.1 (elongator complex protein 5 isoform X2 [Cucurbita moschata])

HSP 1 Score: 722.6 bits (1864), Expect = 1.7e-204
Identity = 367/375 (97.87%), Postives = 372/375 (99.20%), Query Frame = 0

Query: 1   MADSICRALRDGALEGELAPALTLKDSINSPFGFHAFAHVVMQLSSNILAGKSQSRGLVL 60
           MADSICRALRDGALEGELAPALTLKDSINSPFGFHAFAHV+MQLSSNILAGKSQSRGLVL
Sbjct: 1   MADSICRALRDGALEGELAPALTLKDSINSPFGFHAFAHVLMQLSSNILAGKSQSRGLVL 60

Query: 61  LAFSRSPAYYLQLLKKSGVNVGSSEKWIQILDCYTDPLGWKERFTGGEKFSNVHQEVSNL 120
           LAFSRSPAYYL+LLKKSGVNVGSSEKWIQILDCYTDPLGWKERFTGGEK SNVHQEVSNL
Sbjct: 61  LAFSRSPAYYLELLKKSGVNVGSSEKWIQILDCYTDPLGWKERFTGGEKISNVHQEVSNL 120

Query: 121 SHLCTNVRDMDNLFSSIITLGKGFVGEGTVRFCVAIDSVTDMLRHSSTSAVAGLLSNLRS 180
           SHLCTNVRDMDNLFSSIITLGKGFVGEGTVRFCVA+DSVTDMLRHSSTSAVAGLLSNLRS
Sbjct: 121 SHLCTNVRDMDNLFSSIITLGKGFVGEGTVRFCVAVDSVTDMLRHSSTSAVAGLLSNLRS 180

Query: 181 NDKVSSTFWLVHEDLHEEKVIAALEYMSSMVATVEPLTPSPYVRESNLNTPYLEHSSTKG 240
           NDKVSSTFWLVHEDLHEEKVIAALEYMSSMVATVEPLTPSPYVRES+LNTPYLEHSSTKG
Sbjct: 181 NDKVSSTFWLVHEDLHEEKVIAALEYMSSMVATVEPLTPSPYVRESSLNTPYLEHSSTKG 240

Query: 241 RFHVRIKRRNGRVRVICENFKVEQSGIKFTSISSEDAVVNQGLVPKVQFNLQLSEKEQID 300
           RFHVRIKRRNGRVRVICENFKVEQSGIKFTSISSEDAVVNQGLVPKVQFNLQLSEKEQID
Sbjct: 241 RFHVRIKRRNGRVRVICENFKVEQSGIKFTSISSEDAVVNQGLVPKVQFNLQLSEKEQID 300

Query: 301 RARVVLPFEHQGNGKPIQIYDGRRSLTESKDDDKPLSTSEKSKDEGAGKGEIIYFRDSED 360
           RARVVLPFEHQGNGKPIQIYDGRRSLTESKDDDKPLST+EKSKDE  GKGEIIYFRDSED
Sbjct: 301 RARVVLPFEHQGNGKPIQIYDGRRSLTESKDDDKPLSTNEKSKDEEPGKGEIIYFRDSED 360

Query: 361 EMPDSDEDPDDDLDI 376
           EMPDSDEDPDDDLDI
Sbjct: 361 EMPDSDEDPDDDLDI 375

BLAST of Carg07500 vs. NCBI nr
Match: XP_022998087.1 (elongator complex protein 5 isoform X2 [Cucurbita maxima])

HSP 1 Score: 720.7 bits (1859), Expect = 6.5e-204
Identity = 367/375 (97.87%), Postives = 371/375 (98.93%), Query Frame = 0

Query: 1   MADSICRALRDGALEGELAPALTLKDSINSPFGFHAFAHVVMQLSSNILAGKSQSRGLVL 60
           MADSICRALRDGALEGELAPALTLKDSINSPFGFHAFAHV+MQLSSNILAGKSQSRGLVL
Sbjct: 1   MADSICRALRDGALEGELAPALTLKDSINSPFGFHAFAHVLMQLSSNILAGKSQSRGLVL 60

Query: 61  LAFSRSPAYYLQLLKKSGVNVGSSEKWIQILDCYTDPLGWKERFTGGEKFSNVHQEVSNL 120
           LAFSRSPAYYL+LLKKSG NVGSSEKWIQILDCYTDPLGWKERFTGGEK SNVHQEVSNL
Sbjct: 61  LAFSRSPAYYLELLKKSGGNVGSSEKWIQILDCYTDPLGWKERFTGGEKISNVHQEVSNL 120

Query: 121 SHLCTNVRDMDNLFSSIITLGKGFVGEGTVRFCVAIDSVTDMLRHSSTSAVAGLLSNLRS 180
           SHLCTNVRDMDNLFSSIITLGKGFVGEGTVRFCVAIDSVTDMLRHSSTSAVAGLLSN RS
Sbjct: 121 SHLCTNVRDMDNLFSSIITLGKGFVGEGTVRFCVAIDSVTDMLRHSSTSAVAGLLSNFRS 180

Query: 181 NDKVSSTFWLVHEDLHEEKVIAALEYMSSMVATVEPLTPSPYVRESNLNTPYLEHSSTKG 240
           NDKVSSTFWLVHEDLHEEKVIAALEYMSSMVATVEPLTPSPYV ES+LNTPYLEHSSTKG
Sbjct: 181 NDKVSSTFWLVHEDLHEEKVIAALEYMSSMVATVEPLTPSPYVCESSLNTPYLEHSSTKG 240

Query: 241 RFHVRIKRRNGRVRVICENFKVEQSGIKFTSISSEDAVVNQGLVPKVQFNLQLSEKEQID 300
           RFHVRIKRRNGRVRVICENFKVEQSGIKFTSISSEDAVVNQGLVPKVQFNLQLSEKEQID
Sbjct: 241 RFHVRIKRRNGRVRVICENFKVEQSGIKFTSISSEDAVVNQGLVPKVQFNLQLSEKEQID 300

Query: 301 RARVVLPFEHQGNGKPIQIYDGRRSLTESKDDDKPLSTSEKSKDEGAGKGEIIYFRDSED 360
           RARVVLPFEHQGNGKPIQIYDGRRSLT+SKDDDKPLSTSEKSKDEGAGKGEIIYFRDSED
Sbjct: 301 RARVVLPFEHQGNGKPIQIYDGRRSLTDSKDDDKPLSTSEKSKDEGAGKGEIIYFRDSED 360

Query: 361 EMPDSDEDPDDDLDI 376
           EMPDSDEDPDDDLDI
Sbjct: 361 EMPDSDEDPDDDLDI 375

BLAST of Carg07500 vs. ExPASy Swiss-Prot
Match: F4IQJ2 (Elongator complex protein 5 OS=Arabidopsis thaliana OX=3702 GN=ELP5 PE=1 SV=1)

HSP 1 Score: 391.0 bits (1003), Expect = 1.6e-107
Identity = 216/380 (56.84%), Postives = 275/380 (72.37%), Query Frame = 0

Query: 1   MADSICRALRDGALEGELAPALTLKDSINSPFGFHAFAHVVMQLSSNILAGKSQSRGLVL 60
           MA+SI R LRDG  EGELAPALT+++++ SPFG     +++  LSS+ILAGKS S+GLVL
Sbjct: 1   MAESIFRKLRDGGEEGELAPALTIEETVASPFGLDVSGYLLTNLSSSILAGKSSSQGLVL 60

Query: 61  LAFSRSPAYYLQLLKKSGVNVGSSEKWIQILDCYTDPLGWKERFTGGEKFSNVHQEVSNL 120
           + FSRSP++YLQLLK+ G+ V SS KWI+ILDCYTDPLGW       ++ S    E S+L
Sbjct: 61  ITFSRSPSFYLQLLKQKGIVVSSSSKWIRILDCYTDPLGWI------DQSSTSFSEGSSL 120

Query: 121 SHLCTNVRDMDNLFSSIITLGKGFVGEGTVRFCVAIDSVTDMLRHSSTSAVAGLLSNLRS 180
             L   V D+  LFSSII  G+  VG G  RFCVAIDSV ++LRHS+   V+GLL++LRS
Sbjct: 121 IKLHKCVSDLKKLFSSIIEAGRELVGTGKTRFCVAIDSVNELLRHSAMPLVSGLLTDLRS 180

Query: 181 NDKVSSTFWLVHEDLHEEKVIAALEYMSSMVATVEPLTPSPYVRESNLNTPYLEHSS-TK 240
           + ++SS FW ++ DLH+EKV  ALEY+S+M A +EPL PS   + + L   +  H    K
Sbjct: 181 HAQISSVFWSLNTDLHQEKVTNALEYISTMKANLEPLCPSSDGQRNALENLFSVHQDFGK 240

Query: 241 GRFHVRIKRRNGRVRVICENFKVEQSGIKFTSISSEDAVV--NQGLVPKVQFNLQLSEKE 300
           GRFHVR K R GRVRV+ E + V+QSGI F+ ISS D V+   + L+PKVQFNLQLSEKE
Sbjct: 241 GRFHVRFKLRKGRVRVMSEEYHVDQSGINFSPISSVDTVIAATKSLLPKVQFNLQLSEKE 300

Query: 301 QIDRARVVLPFEHQGNGKPIQIYDGRRSLTESKDDDKPLSTSEKSKD-EGAGK-GEIIYF 360
           ++++ +VVLPFEHQ +GK  +IYDGRRSL + K +  PLS+ E   D   +GK GEIIYF
Sbjct: 301 RVEKEKVVLPFEHQDDGKSNEIYDGRRSLVDGKIETTPLSSMELQTDVVSSGKGGEIIYF 360

Query: 361 RDSEDEMPDSDEDPDDDLDI 376
           RDS+DE PDSDEDPDDDLDI
Sbjct: 361 RDSDDEHPDSDEDPDDDLDI 374

BLAST of Carg07500 vs. ExPASy Swiss-Prot
Match: A1A5V9 (Elongator complex protein 5 OS=Danio rerio OX=7955 GN=elp5 PE=2 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 4.1e-07
Identity = 67/290 (23.10%), Postives = 113/290 (38.97%), Query Frame = 0

Query: 94  YTDPLGWKERFTGGEKFSNVHQEVSNLSHLCTNVRDMDNLFSSIITLGKGFVGEGTVRFC 153
           + DPLGW+     G+    V Q  S   H+   +RD     +S++               
Sbjct: 73  FPDPLGWR-----GKSSFTVQQFTS--QHITQLIRDSQPAKASVL--------------- 132

Query: 154 VAIDSVTDMLRHSSTSAVAGLLSNLRSNDKVSSTFWLVHEDLHEEKVIAALEYMSSMVAT 213
             +DS++ +LRH     V   L  LR    V +   L+H DLH + ++  + +++S V +
Sbjct: 133 -VVDSLSLVLRHHDPVIVCQSLQELRKGGVVKTIIGLLHSDLHLQGIVGIVCHLASTVIS 192

Query: 214 VEPLTPSPYVRESNLNTPYLEHSSTKGRFHVRIKRRNGRVRVICENFKVEQSGI------ 273
           V P            N  +   ++T+       + ++G+V    E F V +         
Sbjct: 193 VAPTN----------NERHAVATTTR-------RTKSGKVMQEEEYFSVSEDATLSVQSK 252

Query: 274 --KFTSISSEDAVVNQGLVPKVQFNLQLSEKEQIDRARVVLPFEHQGNGKPIQIYDGRRS 333
             +   +  E           + FNL+LSE+E+  + +V LPF      K   +      
Sbjct: 253 PRQHDRVEKEQDSAEVDPASNLTFNLRLSEEERRAKEKVALPFVFSQEKKSALL------ 296

Query: 334 LTESKDDDKPLSTSEKSKDEGAGKGEIIYFRDSEDEMPDSDEDPDDDLDI 376
                   +P            G G I+Y  D+ D+    +EDPDDDLD+
Sbjct: 313 --------RPT----------PGSGRIMYEPDANDDF--DEEDPDDDLDV 296

BLAST of Carg07500 vs. ExPASy TrEMBL
Match: A0A6J1G9J7 (Elongator complex protein 5 OS=Cucurbita moschata OX=3662 GN=LOC111452202 PE=3 SV=1)

HSP 1 Score: 722.6 bits (1864), Expect = 8.3e-205
Identity = 367/375 (97.87%), Postives = 372/375 (99.20%), Query Frame = 0

Query: 1   MADSICRALRDGALEGELAPALTLKDSINSPFGFHAFAHVVMQLSSNILAGKSQSRGLVL 60
           MADSICRALRDGALEGELAPALTLKDSINSPFGFHAFAHV+MQLSSNILAGKSQSRGLVL
Sbjct: 1   MADSICRALRDGALEGELAPALTLKDSINSPFGFHAFAHVLMQLSSNILAGKSQSRGLVL 60

Query: 61  LAFSRSPAYYLQLLKKSGVNVGSSEKWIQILDCYTDPLGWKERFTGGEKFSNVHQEVSNL 120
           LAFSRSPAYYL+LLKKSGVNVGSSEKWIQILDCYTDPLGWKERFTGGEK SNVHQEVSNL
Sbjct: 61  LAFSRSPAYYLELLKKSGVNVGSSEKWIQILDCYTDPLGWKERFTGGEKISNVHQEVSNL 120

Query: 121 SHLCTNVRDMDNLFSSIITLGKGFVGEGTVRFCVAIDSVTDMLRHSSTSAVAGLLSNLRS 180
           SHLCTNVRDMDNLFSSIITLGKGFVGEGTVRFCVA+DSVTDMLRHSSTSAVAGLLSNLRS
Sbjct: 121 SHLCTNVRDMDNLFSSIITLGKGFVGEGTVRFCVAVDSVTDMLRHSSTSAVAGLLSNLRS 180

Query: 181 NDKVSSTFWLVHEDLHEEKVIAALEYMSSMVATVEPLTPSPYVRESNLNTPYLEHSSTKG 240
           NDKVSSTFWLVHEDLHEEKVIAALEYMSSMVATVEPLTPSPYVRES+LNTPYLEHSSTKG
Sbjct: 181 NDKVSSTFWLVHEDLHEEKVIAALEYMSSMVATVEPLTPSPYVRESSLNTPYLEHSSTKG 240

Query: 241 RFHVRIKRRNGRVRVICENFKVEQSGIKFTSISSEDAVVNQGLVPKVQFNLQLSEKEQID 300
           RFHVRIKRRNGRVRVICENFKVEQSGIKFTSISSEDAVVNQGLVPKVQFNLQLSEKEQID
Sbjct: 241 RFHVRIKRRNGRVRVICENFKVEQSGIKFTSISSEDAVVNQGLVPKVQFNLQLSEKEQID 300

Query: 301 RARVVLPFEHQGNGKPIQIYDGRRSLTESKDDDKPLSTSEKSKDEGAGKGEIIYFRDSED 360
           RARVVLPFEHQGNGKPIQIYDGRRSLTESKDDDKPLST+EKSKDE  GKGEIIYFRDSED
Sbjct: 301 RARVVLPFEHQGNGKPIQIYDGRRSLTESKDDDKPLSTNEKSKDEEPGKGEIIYFRDSED 360

Query: 361 EMPDSDEDPDDDLDI 376
           EMPDSDEDPDDDLDI
Sbjct: 361 EMPDSDEDPDDDLDI 375

BLAST of Carg07500 vs. ExPASy TrEMBL
Match: A0A6J1K6X0 (Elongator complex protein 5 OS=Cucurbita maxima OX=3661 GN=LOC111492840 PE=3 SV=1)

HSP 1 Score: 720.7 bits (1859), Expect = 3.2e-204
Identity = 367/375 (97.87%), Postives = 371/375 (98.93%), Query Frame = 0

Query: 1   MADSICRALRDGALEGELAPALTLKDSINSPFGFHAFAHVVMQLSSNILAGKSQSRGLVL 60
           MADSICRALRDGALEGELAPALTLKDSINSPFGFHAFAHV+MQLSSNILAGKSQSRGLVL
Sbjct: 1   MADSICRALRDGALEGELAPALTLKDSINSPFGFHAFAHVLMQLSSNILAGKSQSRGLVL 60

Query: 61  LAFSRSPAYYLQLLKKSGVNVGSSEKWIQILDCYTDPLGWKERFTGGEKFSNVHQEVSNL 120
           LAFSRSPAYYL+LLKKSG NVGSSEKWIQILDCYTDPLGWKERFTGGEK SNVHQEVSNL
Sbjct: 61  LAFSRSPAYYLELLKKSGGNVGSSEKWIQILDCYTDPLGWKERFTGGEKISNVHQEVSNL 120

Query: 121 SHLCTNVRDMDNLFSSIITLGKGFVGEGTVRFCVAIDSVTDMLRHSSTSAVAGLLSNLRS 180
           SHLCTNVRDMDNLFSSIITLGKGFVGEGTVRFCVAIDSVTDMLRHSSTSAVAGLLSN RS
Sbjct: 121 SHLCTNVRDMDNLFSSIITLGKGFVGEGTVRFCVAIDSVTDMLRHSSTSAVAGLLSNFRS 180

Query: 181 NDKVSSTFWLVHEDLHEEKVIAALEYMSSMVATVEPLTPSPYVRESNLNTPYLEHSSTKG 240
           NDKVSSTFWLVHEDLHEEKVIAALEYMSSMVATVEPLTPSPYV ES+LNTPYLEHSSTKG
Sbjct: 181 NDKVSSTFWLVHEDLHEEKVIAALEYMSSMVATVEPLTPSPYVCESSLNTPYLEHSSTKG 240

Query: 241 RFHVRIKRRNGRVRVICENFKVEQSGIKFTSISSEDAVVNQGLVPKVQFNLQLSEKEQID 300
           RFHVRIKRRNGRVRVICENFKVEQSGIKFTSISSEDAVVNQGLVPKVQFNLQLSEKEQID
Sbjct: 241 RFHVRIKRRNGRVRVICENFKVEQSGIKFTSISSEDAVVNQGLVPKVQFNLQLSEKEQID 300

Query: 301 RARVVLPFEHQGNGKPIQIYDGRRSLTESKDDDKPLSTSEKSKDEGAGKGEIIYFRDSED 360
           RARVVLPFEHQGNGKPIQIYDGRRSLT+SKDDDKPLSTSEKSKDEGAGKGEIIYFRDSED
Sbjct: 301 RARVVLPFEHQGNGKPIQIYDGRRSLTDSKDDDKPLSTSEKSKDEGAGKGEIIYFRDSED 360

Query: 361 EMPDSDEDPDDDLDI 376
           EMPDSDEDPDDDLDI
Sbjct: 361 EMPDSDEDPDDDLDI 375

BLAST of Carg07500 vs. ExPASy TrEMBL
Match: A0A6J1GA78 (Elongator complex protein 5 OS=Cucurbita moschata OX=3662 GN=LOC111452202 PE=3 SV=1)

HSP 1 Score: 718.0 bits (1852), Expect = 2.1e-203
Identity = 367/376 (97.61%), Postives = 372/376 (98.94%), Query Frame = 0

Query: 1   MADSICRALRDGALEGELAPALTLKDSINSPFGFHAFAHVVMQLSSNILAGKSQSRGLVL 60
           MADSICRALRDGALEGELAPALTLKDSINSPFGFHAFAHV+MQLSSNILAGKSQSRGLVL
Sbjct: 1   MADSICRALRDGALEGELAPALTLKDSINSPFGFHAFAHVLMQLSSNILAGKSQSRGLVL 60

Query: 61  LAFSRSPAYYLQLLKKSGVNVGSSEKWIQILDCYTDPLGWKERFTGGEKFSNVHQEVSNL 120
           LAFSRSPAYYL+LLKKSGVNVGSSEKWIQILDCYTDPLGWKERFTGGEK SNVHQEVSNL
Sbjct: 61  LAFSRSPAYYLELLKKSGVNVGSSEKWIQILDCYTDPLGWKERFTGGEKISNVHQEVSNL 120

Query: 121 SHLCTNVRDMDNLFSSIITLGK-GFVGEGTVRFCVAIDSVTDMLRHSSTSAVAGLLSNLR 180
           SHLCTNVRDMDNLFSSIITLGK GFVGEGTVRFCVA+DSVTDMLRHSSTSAVAGLLSNLR
Sbjct: 121 SHLCTNVRDMDNLFSSIITLGKAGFVGEGTVRFCVAVDSVTDMLRHSSTSAVAGLLSNLR 180

Query: 181 SNDKVSSTFWLVHEDLHEEKVIAALEYMSSMVATVEPLTPSPYVRESNLNTPYLEHSSTK 240
           SNDKVSSTFWLVHEDLHEEKVIAALEYMSSMVATVEPLTPSPYVRES+LNTPYLEHSSTK
Sbjct: 181 SNDKVSSTFWLVHEDLHEEKVIAALEYMSSMVATVEPLTPSPYVRESSLNTPYLEHSSTK 240

Query: 241 GRFHVRIKRRNGRVRVICENFKVEQSGIKFTSISSEDAVVNQGLVPKVQFNLQLSEKEQI 300
           GRFHVRIKRRNGRVRVICENFKVEQSGIKFTSISSEDAVVNQGLVPKVQFNLQLSEKEQI
Sbjct: 241 GRFHVRIKRRNGRVRVICENFKVEQSGIKFTSISSEDAVVNQGLVPKVQFNLQLSEKEQI 300

Query: 301 DRARVVLPFEHQGNGKPIQIYDGRRSLTESKDDDKPLSTSEKSKDEGAGKGEIIYFRDSE 360
           DRARVVLPFEHQGNGKPIQIYDGRRSLTESKDDDKPLST+EKSKDE  GKGEIIYFRDSE
Sbjct: 301 DRARVVLPFEHQGNGKPIQIYDGRRSLTESKDDDKPLSTNEKSKDEEPGKGEIIYFRDSE 360

Query: 361 DEMPDSDEDPDDDLDI 376
           DEMPDSDEDPDDDLDI
Sbjct: 361 DEMPDSDEDPDDDLDI 376

BLAST of Carg07500 vs. ExPASy TrEMBL
Match: A0A6J1K998 (Elongator complex protein 5 OS=Cucurbita maxima OX=3661 GN=LOC111492840 PE=3 SV=1)

HSP 1 Score: 716.1 bits (1847), Expect = 7.8e-203
Identity = 367/376 (97.61%), Postives = 371/376 (98.67%), Query Frame = 0

Query: 1   MADSICRALRDGALEGELAPALTLKDSINSPFGFHAFAHVVMQLSSNILAGKSQSRGLVL 60
           MADSICRALRDGALEGELAPALTLKDSINSPFGFHAFAHV+MQLSSNILAGKSQSRGLVL
Sbjct: 1   MADSICRALRDGALEGELAPALTLKDSINSPFGFHAFAHVLMQLSSNILAGKSQSRGLVL 60

Query: 61  LAFSRSPAYYLQLLKKSGVNVGSSEKWIQILDCYTDPLGWKERFTGGEKFSNVHQEVSNL 120
           LAFSRSPAYYL+LLKKSG NVGSSEKWIQILDCYTDPLGWKERFTGGEK SNVHQEVSNL
Sbjct: 61  LAFSRSPAYYLELLKKSGGNVGSSEKWIQILDCYTDPLGWKERFTGGEKISNVHQEVSNL 120

Query: 121 SHLCTNVRDMDNLFSSIITLGK-GFVGEGTVRFCVAIDSVTDMLRHSSTSAVAGLLSNLR 180
           SHLCTNVRDMDNLFSSIITLGK GFVGEGTVRFCVAIDSVTDMLRHSSTSAVAGLLSN R
Sbjct: 121 SHLCTNVRDMDNLFSSIITLGKAGFVGEGTVRFCVAIDSVTDMLRHSSTSAVAGLLSNFR 180

Query: 181 SNDKVSSTFWLVHEDLHEEKVIAALEYMSSMVATVEPLTPSPYVRESNLNTPYLEHSSTK 240
           SNDKVSSTFWLVHEDLHEEKVIAALEYMSSMVATVEPLTPSPYV ES+LNTPYLEHSSTK
Sbjct: 181 SNDKVSSTFWLVHEDLHEEKVIAALEYMSSMVATVEPLTPSPYVCESSLNTPYLEHSSTK 240

Query: 241 GRFHVRIKRRNGRVRVICENFKVEQSGIKFTSISSEDAVVNQGLVPKVQFNLQLSEKEQI 300
           GRFHVRIKRRNGRVRVICENFKVEQSGIKFTSISSEDAVVNQGLVPKVQFNLQLSEKEQI
Sbjct: 241 GRFHVRIKRRNGRVRVICENFKVEQSGIKFTSISSEDAVVNQGLVPKVQFNLQLSEKEQI 300

Query: 301 DRARVVLPFEHQGNGKPIQIYDGRRSLTESKDDDKPLSTSEKSKDEGAGKGEIIYFRDSE 360
           DRARVVLPFEHQGNGKPIQIYDGRRSLT+SKDDDKPLSTSEKSKDEGAGKGEIIYFRDSE
Sbjct: 301 DRARVVLPFEHQGNGKPIQIYDGRRSLTDSKDDDKPLSTSEKSKDEGAGKGEIIYFRDSE 360

Query: 361 DEMPDSDEDPDDDLDI 376
           DEMPDSDEDPDDDLDI
Sbjct: 361 DEMPDSDEDPDDDLDI 376

BLAST of Carg07500 vs. ExPASy TrEMBL
Match: A0A6J1CQU5 (Elongator complex protein 5 OS=Momordica charantia OX=3673 GN=LOC111013317 PE=3 SV=1)

HSP 1 Score: 636.3 bits (1640), Expect = 7.8e-179
Identity = 319/376 (84.84%), Postives = 349/376 (92.82%), Query Frame = 0

Query: 1   MADSICRALRDGALEGELAPALTLKDSINSPFGFHAFAHVVMQLSSNILAGKSQSRGLVL 60
           MA+SICRALRDGALEGELAPALT+KDS++SPFGFHAFAH++ QLS NILAGKSQSRGLVL
Sbjct: 1   MAESICRALRDGALEGELAPALTIKDSMDSPFGFHAFAHLLAQLSINILAGKSQSRGLVL 60

Query: 61  LAFSRSPAYYLQLLKKSGVNVGSSEKWIQILDCYTDPLGWKERFTGGEKFSNVHQEVSNL 120
           LAFSRSPAYY+ LLKK GV+VGSS+KWIQILDCYTDPLGWKER   GEK SNVHQEVS+L
Sbjct: 61  LAFSRSPAYYVDLLKKRGVDVGSSDKWIQILDCYTDPLGWKERLMEGEKVSNVHQEVSSL 120

Query: 121 SHLCTNVRDMDNLFSSIITLGKGFVGEGTVRFCVAIDSVTDMLRHSSTSAVAGLLSNLRS 180
           S+LCTNVRDMD LFSSI+ LGKGFVGEGTVRFCVAIDSVTDMLRHSSTSAVAGLLS+LRS
Sbjct: 121 SNLCTNVRDMDKLFSSIVALGKGFVGEGTVRFCVAIDSVTDMLRHSSTSAVAGLLSSLRS 180

Query: 181 NDKVSSTFWLVHEDLHEEKVIAALEYMSSMVATVEPLTPSPYVRESNLNTPYLEHSSTKG 240
           NDKVSSTFWLVHEDLHEE+VIAALEYMSS+VA+VEP TPSPYV   NL+  Y EH+ST G
Sbjct: 181 NDKVSSTFWLVHEDLHEERVIAALEYMSSIVASVEPSTPSPYVHRGNLDNSYPEHNSTNG 240

Query: 241 RFHVRIKRRNGRVRVICENFKVEQSG-IKFTSISSEDAVVNQGLVPKVQFNLQLSEKEQI 300
           RFHVR KRRNGRVR+ICE+FKVE SG IKFTSI SEDA++NQGL+PKV FNLQLSEKE +
Sbjct: 241 RFHVRFKRRNGRVRIICEDFKVEHSGIIKFTSILSEDAIINQGLIPKVHFNLQLSEKELM 300

Query: 301 DRARVVLPFEHQGNGKPIQIYDGRRSLTESKDDDKPLSTSEKSKDEGAGKGEIIYFRDSE 360
           DRA+VVLPFEHQGNGKPIQIYDGRRSLTESK+DDKPL TSEK KDEG+GKGEI+YFRDS+
Sbjct: 301 DRAKVVLPFEHQGNGKPIQIYDGRRSLTESKEDDKPLLTSEKGKDEGSGKGEIVYFRDSD 360

Query: 361 DEMPDSDEDPDDDLDI 376
           DE PDSDEDPD+DLDI
Sbjct: 361 DERPDSDEDPDEDLDI 376

BLAST of Carg07500 vs. TAIR 10
Match: AT2G18410.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Histone acetylation protein 2 (InterPro:IPR019519); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 391.0 bits (1003), Expect = 1.1e-108
Identity = 216/380 (56.84%), Postives = 275/380 (72.37%), Query Frame = 0

Query: 1   MADSICRALRDGALEGELAPALTLKDSINSPFGFHAFAHVVMQLSSNILAGKSQSRGLVL 60
           MA+SI R LRDG  EGELAPALT+++++ SPFG     +++  LSS+ILAGKS S+GLVL
Sbjct: 1   MAESIFRKLRDGGEEGELAPALTIEETVASPFGLDVSGYLLTNLSSSILAGKSSSQGLVL 60

Query: 61  LAFSRSPAYYLQLLKKSGVNVGSSEKWIQILDCYTDPLGWKERFTGGEKFSNVHQEVSNL 120
           + FSRSP++YLQLLK+ G+ V SS KWI+ILDCYTDPLGW       ++ S    E S+L
Sbjct: 61  ITFSRSPSFYLQLLKQKGIVVSSSSKWIRILDCYTDPLGWI------DQSSTSFSEGSSL 120

Query: 121 SHLCTNVRDMDNLFSSIITLGKGFVGEGTVRFCVAIDSVTDMLRHSSTSAVAGLLSNLRS 180
             L   V D+  LFSSII  G+  VG G  RFCVAIDSV ++LRHS+   V+GLL++LRS
Sbjct: 121 IKLHKCVSDLKKLFSSIIEAGRELVGTGKTRFCVAIDSVNELLRHSAMPLVSGLLTDLRS 180

Query: 181 NDKVSSTFWLVHEDLHEEKVIAALEYMSSMVATVEPLTPSPYVRESNLNTPYLEHSS-TK 240
           + ++SS FW ++ DLH+EKV  ALEY+S+M A +EPL PS   + + L   +  H    K
Sbjct: 181 HAQISSVFWSLNTDLHQEKVTNALEYISTMKANLEPLCPSSDGQRNALENLFSVHQDFGK 240

Query: 241 GRFHVRIKRRNGRVRVICENFKVEQSGIKFTSISSEDAVV--NQGLVPKVQFNLQLSEKE 300
           GRFHVR K R GRVRV+ E + V+QSGI F+ ISS D V+   + L+PKVQFNLQLSEKE
Sbjct: 241 GRFHVRFKLRKGRVRVMSEEYHVDQSGINFSPISSVDTVIAATKSLLPKVQFNLQLSEKE 300

Query: 301 QIDRARVVLPFEHQGNGKPIQIYDGRRSLTESKDDDKPLSTSEKSKD-EGAGK-GEIIYF 360
           ++++ +VVLPFEHQ +GK  +IYDGRRSL + K +  PLS+ E   D   +GK GEIIYF
Sbjct: 301 RVEKEKVVLPFEHQDDGKSNEIYDGRRSLVDGKIETTPLSSMELQTDVVSSGKGGEIIYF 360

Query: 361 RDSEDEMPDSDEDPDDDLDI 376
           RDS+DE PDSDEDPDDDLDI
Sbjct: 361 RDSDDEHPDSDEDPDDDLDI 374

BLAST of Carg07500 vs. TAIR 10
Match: AT2G18410.2 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 287.0 bits (733), Expect = 2.2e-77
Identity = 155/292 (53.08%), Postives = 203/292 (69.52%), Query Frame = 0

Query: 1   MADSICRALRDGALEGELAPALTLKDSINSPFGFHAFAHVVMQLSSNILAGKSQSRGLVL 60
           MA+SI R LRDG  EGELAPALT+++++ SPFG     +++  LSS+ILAGKS S+GLVL
Sbjct: 1   MAESIFRKLRDGGEEGELAPALTIEETVASPFGLDVSGYLLTNLSSSILAGKSSSQGLVL 60

Query: 61  LAFSRSPAYYLQLLKKSGVNVGSSEKWIQILDCYTDPLGWKERFTGGEKFSNVHQEVSNL 120
           + FSRSP++YLQLLK+ G+ V SS KWI+ILDCYTDPLGW       ++ S    E S+L
Sbjct: 61  ITFSRSPSFYLQLLKQKGIVVSSSSKWIRILDCYTDPLGWI------DQSSTSFSEGSSL 120

Query: 121 SHLCTNVRDMDNLFSSIITLGKGFVGEGTVRFCVAIDSVTDMLRHSSTSAVAGLLSNLRS 180
             L   V D+  LFSSII  G+  VG G  RFCVAIDSV ++LRHS+   V+GLL++LRS
Sbjct: 121 IKLHKCVSDLKKLFSSIIEAGRELVGTGKTRFCVAIDSVNELLRHSAMPLVSGLLTDLRS 180

Query: 181 NDKVSSTFWLVHEDLHEEKVIAALEYMSSMVATVEPLTPSPYVRESNLNTPYLEHSS-TK 240
           + ++SS FW ++ DLH+EKV  ALEY+S+M A +EPL PS   + + L   +  H    K
Sbjct: 181 HAQISSVFWSLNTDLHQEKVTNALEYISTMKANLEPLCPSSDGQRNALENLFSVHQDFGK 240

Query: 241 GRFHVRIKRRNGRVRVICENFKVEQSGIKFTSISSEDAVV--NQGLVPKVQF 290
           GRFHVR K R GRVRV+ E + V+QSGI F+ ISS D V+   + L+PK  +
Sbjct: 241 GRFHVRFKLRKGRVRVMSEEYHVDQSGINFSPISSVDTVIAATKSLLPKASY 286

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7036759.12.0e-208100.00Elongator complex protein 5 [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6607058.12.2e-20799.20Elongator complex protein 5, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023521734.17.7e-20597.87elongator complex protein 5 isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_022948561.11.7e-20497.87elongator complex protein 5 isoform X2 [Cucurbita moschata][more]
XP_022998087.16.5e-20497.87elongator complex protein 5 isoform X2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
F4IQJ21.6e-10756.84Elongator complex protein 5 OS=Arabidopsis thaliana OX=3702 GN=ELP5 PE=1 SV=1[more]
A1A5V94.1e-0723.10Elongator complex protein 5 OS=Danio rerio OX=7955 GN=elp5 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1G9J78.3e-20597.87Elongator complex protein 5 OS=Cucurbita moschata OX=3662 GN=LOC111452202 PE=3 S... [more]
A0A6J1K6X03.2e-20497.87Elongator complex protein 5 OS=Cucurbita maxima OX=3661 GN=LOC111492840 PE=3 SV=... [more]
A0A6J1GA782.1e-20397.61Elongator complex protein 5 OS=Cucurbita moschata OX=3662 GN=LOC111452202 PE=3 S... [more]
A0A6J1K9987.8e-20397.61Elongator complex protein 5 OS=Cucurbita maxima OX=3661 GN=LOC111492840 PE=3 SV=... [more]
A0A6J1CQU57.8e-17984.84Elongator complex protein 5 OS=Momordica charantia OX=3673 GN=LOC111013317 PE=3 ... [more]
Match NameE-valueIdentityDescription
AT2G18410.11.1e-10856.84FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow... [more]
AT2G18410.22.2e-7753.08unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 23..218
e-value: 3.1E-5
score: 25.6
IPR019519Elongator complex protein 5PFAMPF10483Elong_Iki1coord: 149..309
e-value: 2.8E-11
score: 43.5
IPR019519Elongator complex protein 5PANTHERPTHR15641ELONGATOR COMPLEX PROTEIN 5coord: 1..375
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 324..359
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 315..375
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 360..375

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg07500-RACarg07500-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0002098 tRNA wobble uridine modification
cellular_component GO:0005829 cytosol
cellular_component GO:0033588 elongator holoenzyme complex
cellular_component GO:0005634 nucleus
molecular_function GO:0000049 tRNA binding