Carg07491 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg07491
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionTPX2 domain-containing protein
LocationCarg_Chr01: 2089886 .. 2091153 (+)
RNA-Seq ExpressionCarg07491
SyntenyCarg07491
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCTCCATCACCCTTTGACCCTCTTCTTCATATCATTCACTCATGGACAAATCTCCACCAAAATCTTTCATCAAGGTTTCCCACTCTTGCTTCATTCTTCTTGTTTCTTTGTTCTTTGCTTTGAACTTTCTTTTCTTCTTGTTCTTAGGCTAAAGATGGCGTTAGAGACCGAGTTTCGGAGAAGACGGAGAGAGTTCGGGTAAGTTTTTCGAGATGGATGTTGAATCTTCTTTGTGGAATGGAAGGAAGTGGTGAGGTTTGATTTGTTTGCTTGTTCTTTTAGACTCCTCAAAAGGTTGTTGTGAAAGATAACACTAAGAAGCCTCAAGATTTCAAGTTGCATACGCAAGAAAGAGCGGTGAAACGAGCGATGTTTAATTACTCGGTGAGTTGAGAATATAAGCAAATATACTTTAACTTATAAACAGAGCGATGATTTAACATCATATAAGAATAGGAGAATTTCGTGTTCAACTGATACTCACATGTCTCGTCTCGTCTTGTCTCGTCTCGTGCTAATCTCGTGATCGTAGATAGCAACGAAGTTATACGTGACAGAGCTACAAAAGAAAGTGGAAGAAAAGTTGCACAAGGTAAACATCAACTTACCAATCTTTTCTACAACACAAAAGTTTGGAATAAAATAGTGAAACAACAAAATGTGTGGAACAGATGATAGAAGAGGAAGAGGTTCGGTTGATGAGAAAGGAGATGATTCCAAGAGCTCAGCTCATGCCCTACTTTGATAGACCCTACTTTCCTCAAAGGTAGAGCACAAAAAGTTGATATAAAAAAAAAAAGCTTTAACGGGTACTTCTCTTTCAAGATTTATAACGTTCGTACTTACAAATACGTAAAAAGTACTTATAGAGTGCTTTGAATGAAGTTCGTATAGCTAAAATACATTAACTTTAAAAGTTGACCTTAAAAAGAACACCTACGTTCCACTCTCAAACGATGTCGTGGTTGTATCACATGCAGATCAAATCGACCTTTGACGATCCCGAGAGAGCCGAGTTTCTTGATGAACAAGGAGCAATGGAGCTGCAACGGTGACAGTGAAGTTTACAGCTTTCAGAGACAAGCTTTGAAACCAATCAAATAAACTATATCATTTGTAAAACCATGTGTTTATGATTGTGTTTGTATAATTTAGTCGTAGACAAAGGGGAGAGTGATGTTTTTGATGTAGAGAGAAGCATTTTCGCTTCCTGACTTGTTCTTTCAAATTCGTCCAAATAAAATGAAACTATCAATCTTTAAAACGAT

mRNA sequence

GCTCCATCACCCTTTGACCCTCTTCTTCATATCATTCACTCATGGACAAATCTCCACCAAAATCTTTCATCAAGGCTAAAGATGGCGTTAGAGACCGAGTTTCGGAGAAGACGGAGAGAGTTCGGACTCCTCAAAAGGTTGTTGTGAAAGATAACACTAAGAAGCCTCAAGATTTCAAGTTGCATACGCAAGAAAGAGCGGTGAAACGAGCGATGTTTAATTACTCGATAGCAACGAAGTTATACGTGACAGAGCTACAAAAGAAAGTGGAAGAAAAGTTGCACAAGATGATAGAAGAGGAAGAGGTTCGGTTGATGAGAAAGGAGATGATTCCAAGAGCTCAGCTCATGCCCTACTTTGATAGACCCTACTTTCCTCAAAGATCAAATCGACCTTTGACGATCCCGAGAGAGCCGAGTTTCTTGATGAACAAGGAGCAATGGAGCTGCAACGGTGACAGTGAAGTTTACAGCTTTCAGAGACAAGCTTTGAAACCAATCAAATAAACTATATCATTTGTAAAACCATGTGTTTATGATTGTGTTTGTATAATTTAGTCGTAGACAAAGGGGAGAGTGATGTTTTTGATGTAGAGAGAAGCATTTTCGCTTCCTGACTTGTTCTTTCAAATTCGTCCAAATAAAATGAAACTATCAATCTTTAAAACGAT

Coding sequence (CDS)

ATGGACAAATCTCCACCAAAATCTTTCATCAAGGCTAAAGATGGCGTTAGAGACCGAGTTTCGGAGAAGACGGAGAGAGTTCGGACTCCTCAAAAGGTTGTTGTGAAAGATAACACTAAGAAGCCTCAAGATTTCAAGTTGCATACGCAAGAAAGAGCGGTGAAACGAGCGATGTTTAATTACTCGATAGCAACGAAGTTATACGTGACAGAGCTACAAAAGAAAGTGGAAGAAAAGTTGCACAAGATGATAGAAGAGGAAGAGGTTCGGTTGATGAGAAAGGAGATGATTCCAAGAGCTCAGCTCATGCCCTACTTTGATAGACCCTACTTTCCTCAAAGATCAAATCGACCTTTGACGATCCCGAGAGAGCCGAGTTTCTTGATGAACAAGGAGCAATGGAGCTGCAACGGTGACAGTGAAGTTTACAGCTTTCAGAGACAAGCTTTGAAACCAATCAAATAA

Protein sequence

MDKSPPKSFIKAKDGVRDRVSEKTERVRTPQKVVVKDNTKKPQDFKLHTQERAVKRAMFNYSIATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPLTIPREPSFLMNKEQWSCNGDSEVYSFQRQALKPIK
Homology
BLAST of Carg07491 vs. NCBI nr
Match: KAG7036751.1 (Protein TPX2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 302.8 bits (774), Expect = 1.7e-78
Identity = 154/154 (100.00%), Postives = 154/154 (100.00%), Query Frame = 0

Query: 1   MDKSPPKSFIKAKDGVRDRVSEKTERVRTPQKVVVKDNTKKPQDFKLHTQERAVKRAMFN 60
           MDKSPPKSFIKAKDGVRDRVSEKTERVRTPQKVVVKDNTKKPQDFKLHTQERAVKRAMFN
Sbjct: 1   MDKSPPKSFIKAKDGVRDRVSEKTERVRTPQKVVVKDNTKKPQDFKLHTQERAVKRAMFN 60

Query: 61  YSIATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPLT 120
           YSIATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPLT
Sbjct: 61  YSIATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPLT 120

Query: 121 IPREPSFLMNKEQWSCNGDSEVYSFQRQALKPIK 155
           IPREPSFLMNKEQWSCNGDSEVYSFQRQALKPIK
Sbjct: 121 IPREPSFLMNKEQWSCNGDSEVYSFQRQALKPIK 154

BLAST of Carg07491 vs. NCBI nr
Match: XP_023525926.1 (protein TPX2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 301.6 bits (771), Expect = 3.9e-78
Identity = 153/154 (99.35%), Postives = 154/154 (100.00%), Query Frame = 0

Query: 1   MDKSPPKSFIKAKDGVRDRVSEKTERVRTPQKVVVKDNTKKPQDFKLHTQERAVKRAMFN 60
           MDKSPPKSFIKAKDGVRDRVS+KTERVRTPQKVVVKDNTKKPQDFKLHTQERAVKRAMFN
Sbjct: 1   MDKSPPKSFIKAKDGVRDRVSDKTERVRTPQKVVVKDNTKKPQDFKLHTQERAVKRAMFN 60

Query: 61  YSIATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPLT 120
           YSIATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPLT
Sbjct: 61  YSIATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPLT 120

Query: 121 IPREPSFLMNKEQWSCNGDSEVYSFQRQALKPIK 155
           IPREPSFLMNKEQWSCNGDSEVYSFQRQALKPIK
Sbjct: 121 IPREPSFLMNKEQWSCNGDSEVYSFQRQALKPIK 154

BLAST of Carg07491 vs. NCBI nr
Match: XP_022949252.1 (protein TPX2-like [Cucurbita moschata])

HSP 1 Score: 298.1 bits (762), Expect = 4.3e-77
Identity = 151/154 (98.05%), Postives = 153/154 (99.35%), Query Frame = 0

Query: 1   MDKSPPKSFIKAKDGVRDRVSEKTERVRTPQKVVVKDNTKKPQDFKLHTQERAVKRAMFN 60
           MDKSPPKSFIKAKDGVRDRVSEKTERVRTPQKVVVK+NTKKPQDFKLHTQERAVKRAMFN
Sbjct: 1   MDKSPPKSFIKAKDGVRDRVSEKTERVRTPQKVVVKENTKKPQDFKLHTQERAVKRAMFN 60

Query: 61  YSIATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPLT 120
           YSIATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPLT
Sbjct: 61  YSIATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPLT 120

Query: 121 IPREPSFLMNKEQWSCNGDSEVYSFQRQALKPIK 155
           IPREPSFL+NKEQWSCNGDSEVYSFQRQ LKPIK
Sbjct: 121 IPREPSFLINKEQWSCNGDSEVYSFQRQTLKPIK 154

BLAST of Carg07491 vs. NCBI nr
Match: XP_022998171.1 (protein TPX2 [Cucurbita maxima])

HSP 1 Score: 294.3 bits (752), Expect = 6.2e-76
Identity = 149/154 (96.75%), Postives = 152/154 (98.70%), Query Frame = 0

Query: 1   MDKSPPKSFIKAKDGVRDRVSEKTERVRTPQKVVVKDNTKKPQDFKLHTQERAVKRAMFN 60
           MDKSPPKSFIKAKDG+RDRVS+KTERVRTPQKVVVK+NTKKPQDFKLHTQERAVKRAMFN
Sbjct: 1   MDKSPPKSFIKAKDGIRDRVSDKTERVRTPQKVVVKENTKKPQDFKLHTQERAVKRAMFN 60

Query: 61  YSIATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPLT 120
           YSIATKLYV ELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPLT
Sbjct: 61  YSIATKLYVMELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPLT 120

Query: 121 IPREPSFLMNKEQWSCNGDSEVYSFQRQALKPIK 155
           IPREPSFLMNKEQWSCN DSEVYSFQRQALKPIK
Sbjct: 121 IPREPSFLMNKEQWSCNTDSEVYSFQRQALKPIK 154

BLAST of Carg07491 vs. NCBI nr
Match: XP_038877019.1 (protein TPX2-like [Benincasa hispida])

HSP 1 Score: 272.3 bits (695), Expect = 2.5e-69
Identity = 143/156 (91.67%), Postives = 147/156 (94.23%), Query Frame = 0

Query: 1   MDKSPPKSFI-KAKDGVRDRVSEKTERVRTPQKVV-VKDNTKKPQDFKLHTQERAVKRAM 60
           MDKS PKSF+ K KDGVRDRV EK ERVR PQKVV VK+NTKKPQDFKLHTQERAVKRAM
Sbjct: 1   MDKSQPKSFMTKTKDGVRDRVLEKAERVRAPQKVVHVKENTKKPQDFKLHTQERAVKRAM 60

Query: 61  FNYSIATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRP 120
           FNYSIATKLYV ELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRP
Sbjct: 61  FNYSIATKLYVMELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRP 120

Query: 121 LTIPREPSFLMNKEQWSCNGDSEVYSFQRQALKPIK 155
           LTIPREPSFLMNKEQW+CN DSE+YSFQRQALKPIK
Sbjct: 121 LTIPREPSFLMNKEQWNCNTDSELYSFQRQALKPIK 156

BLAST of Carg07491 vs. ExPASy Swiss-Prot
Match: F4I2H7 (Protein TPX2 OS=Arabidopsis thaliana OX=3702 GN=TPX2 PE=1 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 9.8e-08
Identity = 41/126 (32.54%), Postives = 70/126 (55.56%), Query Frame = 0

Query: 7   KSFIKAKDGVRDRVSEKTERVRTPQKVVVKDNTKKPQDFKLHTQERAVKRAMFNYSIATK 66
           K   KA+  +++      E+VR P   +        Q+F LH + RAV+RA F++ I  K
Sbjct: 617 KRLFKAQPVIKEDPIPVPEKVRMPLTEI--------QEFNLHVEHRAVERADFDHKIKEK 676

Query: 67  LYVTELQKKVEEKLHKMIEEEE-VRLMRKEMIPRAQLMPYFDRPYFPQRSNRPLTIPREP 126
               +  ++ E +  KM+EEE  ++ MRK M+P A+ +P F++P+ PQ+SN+  T  + P
Sbjct: 677 ENQYKRYRE-ESEAAKMVEEERALKQMRKTMVPHARPVPNFNKPFLPQKSNKGTTKAKSP 733

Query: 127 SFLMNK 132
           +  + K
Sbjct: 737 NLRVIK 733

BLAST of Carg07491 vs. ExPASy TrEMBL
Match: A0A6J1GBJ5 (protein TPX2-like OS=Cucurbita moschata OX=3662 GN=LOC111452663 PE=3 SV=1)

HSP 1 Score: 298.1 bits (762), Expect = 2.1e-77
Identity = 151/154 (98.05%), Postives = 153/154 (99.35%), Query Frame = 0

Query: 1   MDKSPPKSFIKAKDGVRDRVSEKTERVRTPQKVVVKDNTKKPQDFKLHTQERAVKRAMFN 60
           MDKSPPKSFIKAKDGVRDRVSEKTERVRTPQKVVVK+NTKKPQDFKLHTQERAVKRAMFN
Sbjct: 1   MDKSPPKSFIKAKDGVRDRVSEKTERVRTPQKVVVKENTKKPQDFKLHTQERAVKRAMFN 60

Query: 61  YSIATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPLT 120
           YSIATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPLT
Sbjct: 61  YSIATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPLT 120

Query: 121 IPREPSFLMNKEQWSCNGDSEVYSFQRQALKPIK 155
           IPREPSFL+NKEQWSCNGDSEVYSFQRQ LKPIK
Sbjct: 121 IPREPSFLINKEQWSCNGDSEVYSFQRQTLKPIK 154

BLAST of Carg07491 vs. ExPASy TrEMBL
Match: A0A6J1K9I8 (protein TPX2 OS=Cucurbita maxima OX=3661 GN=LOC111492897 PE=3 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 3.0e-76
Identity = 149/154 (96.75%), Postives = 152/154 (98.70%), Query Frame = 0

Query: 1   MDKSPPKSFIKAKDGVRDRVSEKTERVRTPQKVVVKDNTKKPQDFKLHTQERAVKRAMFN 60
           MDKSPPKSFIKAKDG+RDRVS+KTERVRTPQKVVVK+NTKKPQDFKLHTQERAVKRAMFN
Sbjct: 1   MDKSPPKSFIKAKDGIRDRVSDKTERVRTPQKVVVKENTKKPQDFKLHTQERAVKRAMFN 60

Query: 61  YSIATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPLT 120
           YSIATKLYV ELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPLT
Sbjct: 61  YSIATKLYVMELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPLT 120

Query: 121 IPREPSFLMNKEQWSCNGDSEVYSFQRQALKPIK 155
           IPREPSFLMNKEQWSCN DSEVYSFQRQALKPIK
Sbjct: 121 IPREPSFLMNKEQWSCNTDSEVYSFQRQALKPIK 154

BLAST of Carg07491 vs. ExPASy TrEMBL
Match: A0A0A0LCD4 (TPX2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G258180 PE=3 SV=1)

HSP 1 Score: 269.2 bits (687), Expect = 1.0e-68
Identity = 140/155 (90.32%), Postives = 143/155 (92.26%), Query Frame = 0

Query: 1   MDKSPPKSFI-KAKDGVRDRVSEKTERVRTPQKVVVKDNTKKPQDFKLHTQERAVKRAMF 60
           MDKS PKS + K KDGVRDRV EK ER R PQK  VK+NTKK QDFKLHTQERAVKRAMF
Sbjct: 1   MDKSQPKSLMTKTKDGVRDRVLEKAERARVPQKAHVKENTKKSQDFKLHTQERAVKRAMF 60

Query: 61  NYSIATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPL 120
           NYSIATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPL
Sbjct: 61  NYSIATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPL 120

Query: 121 TIPREPSFLMNKEQWSCNGDSEVYSFQRQALKPIK 155
           TIPREPSFLMNKEQWSCN DSE+YSFQRQALKPIK
Sbjct: 121 TIPREPSFLMNKEQWSCNTDSELYSFQRQALKPIK 155

BLAST of Carg07491 vs. ExPASy TrEMBL
Match: A0A6J1CNR5 (protein TPX2 OS=Momordica charantia OX=3673 GN=LOC111013295 PE=3 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 1.1e-65
Identity = 131/154 (85.06%), Postives = 142/154 (92.21%), Query Frame = 0

Query: 1   MDKSPPKSFIKAKDGVRDRVSEKTERVRTPQKVVVKDNTKKPQDFKLHTQERAVKRAMFN 60
           MDK  P+S IK KDGVRDRVSE+ ER R  QKVVVK+NTK  QDFKLHTQERAVKRAMFN
Sbjct: 1   MDKFQPRSLIKVKDGVRDRVSERAERARGSQKVVVKENTKM-QDFKLHTQERAVKRAMFN 60

Query: 61  YSIATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPLT 120
           YS+ATK YVTE+QKK+EEKLHKMIEEEE+RL+RKEM+PRAQLMPYFDRPYFPQRSNRPLT
Sbjct: 61  YSVATKSYVTEIQKKIEEKLHKMIEEEEIRLLRKEMVPRAQLMPYFDRPYFPQRSNRPLT 120

Query: 121 IPREPSFLMNKEQWSCNGDSEVYSFQRQALKPIK 155
           IPREPSFL+NKEQWSCN DSE+YSFQRQALKPIK
Sbjct: 121 IPREPSFLVNKEQWSCNTDSELYSFQRQALKPIK 153

BLAST of Carg07491 vs. ExPASy TrEMBL
Match: A0A5D3DX27 (Protein TPX2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold600G00390 PE=3 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 1.2e-45
Identity = 103/115 (89.57%), Postives = 105/115 (91.30%), Query Frame = 0

Query: 1   MDKSPPKSFI-KAKDGVRDRVSEKTERVRTPQKVVVKDNTKKPQDFKLHTQERAVKRAMF 60
           MDKS PKS + K KDGVRDRV EK ER R PQK  VK+NTKKPQDFKLHTQERAVKRAMF
Sbjct: 1   MDKSQPKSLMTKTKDGVRDRVLEKVERARVPQKAPVKENTKKPQDFKLHTQERAVKRAMF 60

Query: 61  NYSIATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQR 115
           NYSIATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQR
Sbjct: 61  NYSIATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQR 115

BLAST of Carg07491 vs. TAIR 10
Match: AT5G37478.1 (TPX2 (targeting protein for Xklp2) protein family )

HSP 1 Score: 144.8 bits (364), Expect = 5.7e-35
Identity = 75/133 (56.39%), Postives = 103/133 (77.44%), Query Frame = 0

Query: 16  VRDRVSEKTERVRTPQ--KVVVKDNTKKPQDFKLHTQERAVKRAMFNYSIATKLYVTELQ 75
           +++R  +KT+   T    K   K+N KKP +FKLH+ ERAVKRAMFNYS+AT  Y+ +LQ
Sbjct: 40  LKERAHDKTKAATTKNITKAPAKEN-KKPLEFKLHSGERAVKRAMFNYSVATNYYIQKLQ 99

Query: 76  KKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPLTIPREPSF-LMNKE 135
           KK EE+L KMIEEEE+R++RKEM+P+AQLMP+FDRP+ PQRS+RPLT+P+EPSF  +N  
Sbjct: 100 KKQEERLQKMIEEEEIRMLRKEMVPKAQLMPFFDRPFLPQRSSRPLTMPKEPSFGNVNST 159

Query: 136 QWSCNGDSEVYSF 146
            W+C  +++ Y +
Sbjct: 160 CWTCVFNNQHYLY 171

BLAST of Carg07491 vs. TAIR 10
Match: AT3G01015.1 (TPX2 (targeting protein for Xklp2) protein family )

HSP 1 Score: 93.2 bits (230), Expect = 2.0e-19
Identity = 48/93 (51.61%), Postives = 66/93 (70.97%), Query Frame = 0

Query: 35  VKDNTKKPQDFKLHTQERAVKRAMFNYSIATKLYVTELQKKVEEKLHKMIEEEEVRLMRK 94
           VKD T  P D KLH+  RAV+RA F+Y +  K+ + E  K   E+  K+ EEEE+R +RK
Sbjct: 365 VKDIT-IPVDLKLHSDIRAVERAEFDYQVTEKINLVEQYKTERERQQKLAEEEEIRRLRK 424

Query: 95  EMIPRAQLMPYFDRPYFPQRSNRPLTIPREPSF 128
           E++P+AQ MPYFDRP+ P+RSN+  T+PR+P F
Sbjct: 425 ELVPKAQPMPYFDRPFIPKRSNKHPTVPRDPKF 456

BLAST of Carg07491 vs. TAIR 10
Match: AT5G15510.1 (TPX2 (targeting protein for Xklp2) protein family )

HSP 1 Score: 88.6 bits (218), Expect = 4.8e-18
Identity = 46/92 (50.00%), Postives = 64/92 (69.57%), Query Frame = 0

Query: 36  KDNTKKPQDFKLHTQERAVKRAMFNYSIATKLYVTELQKKVEEKLHKMIEEEEVRLMRKE 95
           KD T +P D  LH+  RAV+RA F+Y +A K+   E  K   E+  K+ EEEE+R +RKE
Sbjct: 376 KDIT-RPVDLTLHSDVRAVERAEFDYQVAEKMSFIEQYKMERERQQKLAEEEEIRRLRKE 435

Query: 96  MIPRAQLMPYFDRPYFPQRSNRPLTIPREPSF 128
           ++P+AQ MPYFDRP+ P+RS++  T PR+P F
Sbjct: 436 LVPKAQPMPYFDRPFIPRRSSKHPTAPRDPKF 466

BLAST of Carg07491 vs. TAIR 10
Match: AT5G15510.2 (TPX2 (targeting protein for Xklp2) protein family )

HSP 1 Score: 75.5 bits (184), Expect = 4.2e-14
Identity = 40/79 (50.63%), Postives = 55/79 (69.62%), Query Frame = 0

Query: 36  KDNTKKPQDFKLHTQERAVKRAMFNYSIATKLYVTELQKKVEEKLHKMIEEEEVRLMRKE 95
           KD T +P D  LH+  RAV+RA F+Y +A K+   E  K   E+  K+ EEEE+R +RKE
Sbjct: 376 KDIT-RPVDLTLHSDVRAVERAEFDYQVAEKMSFIEQYKMERERQQKLAEEEEIRRLRKE 435

Query: 96  MIPRAQLMPYFDRPYFPQR 115
           ++P+AQ MPYFDRP+ P+R
Sbjct: 436 LVPKAQPMPYFDRPFIPRR 453

BLAST of Carg07491 vs. TAIR 10
Match: AT1G03780.2 (targeting protein for XKLP2 )

HSP 1 Score: 58.2 bits (139), Expect = 7.0e-09
Identity = 41/126 (32.54%), Postives = 70/126 (55.56%), Query Frame = 0

Query: 7   KSFIKAKDGVRDRVSEKTERVRTPQKVVVKDNTKKPQDFKLHTQERAVKRAMFNYSIATK 66
           K   KA+  +++      E+VR P   +        Q+F LH + RAV+RA F++ I  K
Sbjct: 617 KRLFKAQPVIKEDPIPVPEKVRMPLTEI--------QEFNLHVEHRAVERADFDHKIKEK 676

Query: 67  LYVTELQKKVEEKLHKMIEEEE-VRLMRKEMIPRAQLMPYFDRPYFPQRSNRPLTIPREP 126
               +  ++ E +  KM+EEE  ++ MRK M+P A+ +P F++P+ PQ+SN+  T  + P
Sbjct: 677 ENQYKRYRE-ESEAAKMVEEERALKQMRKTMVPHARPVPNFNKPFLPQKSNKGTTKAKSP 733

Query: 127 SFLMNK 132
           +  + K
Sbjct: 737 NLRVIK 733

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7036751.11.7e-78100.00Protein TPX2 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023525926.13.9e-7899.35protein TPX2-like [Cucurbita pepo subsp. pepo][more]
XP_022949252.14.3e-7798.05protein TPX2-like [Cucurbita moschata][more]
XP_022998171.16.2e-7696.75protein TPX2 [Cucurbita maxima][more]
XP_038877019.12.5e-6991.67protein TPX2-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
F4I2H79.8e-0832.54Protein TPX2 OS=Arabidopsis thaliana OX=3702 GN=TPX2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1GBJ52.1e-7798.05protein TPX2-like OS=Cucurbita moschata OX=3662 GN=LOC111452663 PE=3 SV=1[more]
A0A6J1K9I83.0e-7696.75protein TPX2 OS=Cucurbita maxima OX=3661 GN=LOC111492897 PE=3 SV=1[more]
A0A0A0LCD41.0e-6890.32TPX2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G258180 PE=3 S... [more]
A0A6J1CNR51.1e-6585.06protein TPX2 OS=Momordica charantia OX=3673 GN=LOC111013295 PE=3 SV=1[more]
A0A5D3DX271.2e-4589.57Protein TPX2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold600G00390 P... [more]
Match NameE-valueIdentityDescription
AT5G37478.15.7e-3556.39TPX2 (targeting protein for Xklp2) protein family [more]
AT3G01015.12.0e-1951.61TPX2 (targeting protein for Xklp2) protein family [more]
AT5G15510.14.8e-1850.00TPX2 (targeting protein for Xklp2) protein family [more]
AT5G15510.24.2e-1450.63TPX2 (targeting protein for Xklp2) protein family [more]
AT1G03780.27.0e-0932.54targeting protein for XKLP2 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 69..89
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..31
NoneNo IPR availablePANTHERPTHR14326:SF39TPX2 (TARGETING PROTEIN FOR XKLP2) PROTEIN FAMILYcoord: 12..146
IPR027329TPX2, C-terminalPFAMPF06886TPX2coord: 45..126
e-value: 4.2E-20
score: 71.7
IPR009675TPX2PANTHERPTHR14326TARGETING PROTEIN FOR XKLP2coord: 12..146

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg07491-RACarg07491-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032147 activation of protein kinase activity
biological_process GO:0090307 mitotic spindle assembly
biological_process GO:0060236 regulation of mitotic spindle organization
cellular_component GO:0005737 cytoplasm
cellular_component GO:0072686 mitotic spindle
cellular_component GO:0005880 nuclear microtubule
cellular_component GO:0005874 microtubule
cellular_component GO:0005819 spindle
molecular_function GO:0008017 microtubule binding
molecular_function GO:0030295 protein kinase activator activity