Carg07457 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg07457
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionRING-type E3 ubiquitin transferase
LocationCarg_Chr01: 1927300 .. 1928631 (+)
RNA-Seq ExpressionCarg07457
SyntenyCarg07457
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTCTTGTCATGGAGGAGAAGCAAACTTGGGTTTCTACCCAAGTTGAAGAAAGAGCATCTTCCGGGCGTTGATTCGGGTTCTGAAATTGTGATTCCTTCGCATTTTCTATGTCCGATTTCGCTTGATTTGATGAAGGATCCTGTAATTTTGTCCACCGGGATTACTTATGATAGAGAGAGCATCGAGAAGTGGATTGAGGGTGGGAAGTTTTCTTGTCCTGTGACGAAACAGGATTTGATGAGTTTTGACCTAATCCCTAATCATACCTTGAGGAGGATGATCCAAGATTGGTGCGTTGCGAATCATTCTTACGGAATTGAGCGGATTCCAACACCGCGGATTCCAGTCACGCCGTATGAAGTTAATGACGTTTGTTCGAGAATCGCCGTCGCGACTCAAAGGGGCGATTCGAAGAGATGCAGTGAATTGGTGGGTAAGATCAGGAATTGGGCGAGAGAGAGCGAGAGGAATCGGAGGTGCGTTGTGAATGGAGGAACTGGGATTGTTCTAGCAGCTTCGTTTGAGCATTTTTCTGGTGTTTCGATTGAGAAACATGTGGGTTTGTTGGAGGAGATTTTGTTGGTTTTGGTATGTGTATTCCCAATCTCCATTGAAGGGTTATCCAAGCTCGGATCTGCGGATTCCTTGAAATGCTTGGTTTCCTTCTTAATGGAGAAAAATCTTTCACCAAAACAGAACGCAGTTTTTGTTCTGAAAGAACTGCTTCTGGTTGATCAAAGATACGTCGATTCATTGGCTGCCATTGAAGGAGTATCCGAAGCTTTAGTTAGCATCATAAGGGAGCCTCTTTGCCCTTCAGCTACAAAATCATCCCTTACAGCCATTTTCTACATGATTTTACCATCAGAAATCAGCGAGAAAATGACATTGAAATTCATGGAACTGGGCTTGGTCTCTCAGCTGCTAGATTTCCTTGTAGATGCAGAGAAAAGCCTGTGTGAGAAAGCGTTGGGAGTATTGGATGGAATCTGTGATTTTAAACAAGGAAGAGAGAAGCTATACCAAAATGCACTAACAATTCCATTGTTAGTCAAGAAGATCCTAAGAGTATCAGAGGTTGCCTCTGAGTATTGTCTGTCAATACTATTGAAGCTTTGTAAGAGTGGAGAGAAGGGTGAGGATGAGGCGAGGGTGGAAGCTGCTCAACTGGGTGCTTTCCAGAAGATAATTGTTCTTTTGCAGGTTGGTTGCGGTGGTGACATGAAAAACAAGGTCACTGAGATGTTGAAATTGTTGAATCTTTACAAGGATAGGTTAGATTGTATTGATTCATCCATGCATTTTAAGTTCTTGAAGAAATCATTCTGA

mRNA sequence

ATGTTCTTGTCATGGAGGAGAAGCAAACTTGGGTTTCTACCCAAGTTGAAGAAAGAGCATCTTCCGGGCGTTGATTCGGGTTCTGAAATTGTGATTCCTTCGCATTTTCTATGTCCGATTTCGCTTGATTTGATGAAGGATCCTGTAATTTTGTCCACCGGGATTACTTATGATAGAGAGAGCATCGAGAAGTGGATTGAGGGTGGGAAGTTTTCTTGTCCTGTGACGAAACAGGATTTGATGAGTTTTGACCTAATCCCTAATCATACCTTGAGGAGGATGATCCAAGATTGGTGCGTTGCGAATCATTCTTACGGAATTGAGCGGATTCCAACACCGCGGATTCCAGTCACGCCGTATGAAGTTAATGACGTTTGTTCGAGAATCGCCGTCGCGACTCAAAGGGGCGATTCGAAGAGATGCAGTGAATTGGTGGGTAAGATCAGGAATTGGGCGAGAGAGAGCGAGAGGAATCGGAGGTGCGTTGTGAATGGAGGAACTGGGATTGTTCTAGCAGCTTCGTTTGAGCATTTTTCTGGTGTTTCGATTGAGAAACATGTGGGTTTGTTGGAGGAGATTTTGTTGGTTTTGGTATGTGTATTCCCAATCTCCATTGAAGGGTTATCCAAGCTCGGATCTGCGGATTCCTTGAAATGCTTGGTTTCCTTCTTAATGGAGAAAAATCTTTCACCAAAACAGAACGCAGTTTTTGTTCTGAAAGAACTGCTTCTGGTTGATCAAAGATACGTCGATTCATTGGCTGCCATTGAAGGAGTATCCGAAGCTTTAGTTAGCATCATAAGGGAGCCTCTTTGCCCTTCAGCTACAAAATCATCCCTTACAGCCATTTTCTACATGATTTTACCATCAGAAATCAGCGAGAAAATGACATTGAAATTCATGGAACTGGGCTTGGTCTCTCAGCTGCTAGATTTCCTTGTAGATGCAGAGAAAAGCCTGTGTGAGAAAGCGTTGGGAGTATTGGATGGAATCTGTGATTTTAAACAAGGAAGAGAGAAGCTATACCAAAATGCACTAACAATTCCATTGTTAGTCAAGAAGATCCTAAGAGTATCAGAGGTTGCCTCTGAGTATTGTCTGTCAATACTATTGAAGCTTTGTAAGAGTGGAGAGAAGGGTGAGGATGAGGCGAGGGTGGAAGCTGCTCAACTGGGTGCTTTCCAGAAGATAATTGTTCTTTTGCAGGTTGGTTGCGGTGGTGACATGAAAAACAAGGTCACTGAGATGTTGAAATTGTTGAATCTTTACAAGGATAGGTTAGATTGTATTGATTCATCCATGCATTTTAAGTTCTTGAAGAAATCATTCTGA

Coding sequence (CDS)

ATGTTCTTGTCATGGAGGAGAAGCAAACTTGGGTTTCTACCCAAGTTGAAGAAAGAGCATCTTCCGGGCGTTGATTCGGGTTCTGAAATTGTGATTCCTTCGCATTTTCTATGTCCGATTTCGCTTGATTTGATGAAGGATCCTGTAATTTTGTCCACCGGGATTACTTATGATAGAGAGAGCATCGAGAAGTGGATTGAGGGTGGGAAGTTTTCTTGTCCTGTGACGAAACAGGATTTGATGAGTTTTGACCTAATCCCTAATCATACCTTGAGGAGGATGATCCAAGATTGGTGCGTTGCGAATCATTCTTACGGAATTGAGCGGATTCCAACACCGCGGATTCCAGTCACGCCGTATGAAGTTAATGACGTTTGTTCGAGAATCGCCGTCGCGACTCAAAGGGGCGATTCGAAGAGATGCAGTGAATTGGTGGGTAAGATCAGGAATTGGGCGAGAGAGAGCGAGAGGAATCGGAGGTGCGTTGTGAATGGAGGAACTGGGATTGTTCTAGCAGCTTCGTTTGAGCATTTTTCTGGTGTTTCGATTGAGAAACATGTGGGTTTGTTGGAGGAGATTTTGTTGGTTTTGGTATGTGTATTCCCAATCTCCATTGAAGGGTTATCCAAGCTCGGATCTGCGGATTCCTTGAAATGCTTGGTTTCCTTCTTAATGGAGAAAAATCTTTCACCAAAACAGAACGCAGTTTTTGTTCTGAAAGAACTGCTTCTGGTTGATCAAAGATACGTCGATTCATTGGCTGCCATTGAAGGAGTATCCGAAGCTTTAGTTAGCATCATAAGGGAGCCTCTTTGCCCTTCAGCTACAAAATCATCCCTTACAGCCATTTTCTACATGATTTTACCATCAGAAATCAGCGAGAAAATGACATTGAAATTCATGGAACTGGGCTTGGTCTCTCAGCTGCTAGATTTCCTTGTAGATGCAGAGAAAAGCCTGTGTGAGAAAGCGTTGGGAGTATTGGATGGAATCTGTGATTTTAAACAAGGAAGAGAGAAGCTATACCAAAATGCACTAACAATTCCATTGTTAGTCAAGAAGATCCTAAGAGTATCAGAGGTTGCCTCTGAGTATTGTCTGTCAATACTATTGAAGCTTTGTAAGAGTGGAGAGAAGGGTGAGGATGAGGCGAGGGTGGAAGCTGCTCAACTGGGTGCTTTCCAGAAGATAATTGTTCTTTTGCAGGTTGGTTGCGGTGGTGACATGAAAAACAAGGTCACTGAGATGTTGAAATTGTTGAATCTTTACAAGGATAGGTTAGATTGTATTGATTCATCCATGCATTTTAAGTTCTTGAAGAAATCATTCTGA

Protein sequence

MFLSWRRSKLGFLPKLKKEHLPGVDSGSEIVIPSHFLCPISLDLMKDPVILSTGITYDRESIEKWIEGGKFSCPVTKQDLMSFDLIPNHTLRRMIQDWCVANHSYGIERIPTPRIPVTPYEVNDVCSRIAVATQRGDSKRCSELVGKIRNWARESERNRRCVVNGGTGIVLAASFEHFSGVSIEKHVGLLEEILLVLVCVFPISIEGLSKLGSADSLKCLVSFLMEKNLSPKQNAVFVLKELLLVDQRYVDSLAAIEGVSEALVSIIREPLCPSATKSSLTAIFYMILPSEISEKMTLKFMELGLVSQLLDFLVDAEKSLCEKALGVLDGICDFKQGREKLYQNALTIPLLVKKILRVSEVASEYCLSILLKLCKSGEKGEDEARVEAAQLGAFQKIIVLLQVGCGGDMKNKVTEMLKLLNLYKDRLDCIDSSMHFKFLKKSF
Homology
BLAST of Carg07457 vs. NCBI nr
Match: KAG6607019.1 (U-box domain-containing protein 21, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036717.1 U-box domain-containing protein 21, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 872.5 bits (2253), Expect = 1.6e-249
Identity = 443/443 (100.00%), Postives = 443/443 (100.00%), Query Frame = 0

Query: 1   MFLSWRRSKLGFLPKLKKEHLPGVDSGSEIVIPSHFLCPISLDLMKDPVILSTGITYDRE 60
           MFLSWRRSKLGFLPKLKKEHLPGVDSGSEIVIPSHFLCPISLDLMKDPVILSTGITYDRE
Sbjct: 1   MFLSWRRSKLGFLPKLKKEHLPGVDSGSEIVIPSHFLCPISLDLMKDPVILSTGITYDRE 60

Query: 61  SIEKWIEGGKFSCPVTKQDLMSFDLIPNHTLRRMIQDWCVANHSYGIERIPTPRIPVTPY 120
           SIEKWIEGGKFSCPVTKQDLMSFDLIPNHTLRRMIQDWCVANHSYGIERIPTPRIPVTPY
Sbjct: 61  SIEKWIEGGKFSCPVTKQDLMSFDLIPNHTLRRMIQDWCVANHSYGIERIPTPRIPVTPY 120

Query: 121 EVNDVCSRIAVATQRGDSKRCSELVGKIRNWARESERNRRCVVNGGTGIVLAASFEHFSG 180
           EVNDVCSRIAVATQRGDSKRCSELVGKIRNWARESERNRRCVVNGGTGIVLAASFEHFSG
Sbjct: 121 EVNDVCSRIAVATQRGDSKRCSELVGKIRNWARESERNRRCVVNGGTGIVLAASFEHFSG 180

Query: 181 VSIEKHVGLLEEILLVLVCVFPISIEGLSKLGSADSLKCLVSFLMEKNLSPKQNAVFVLK 240
           VSIEKHVGLLEEILLVLVCVFPISIEGLSKLGSADSLKCLVSFLMEKNLSPKQNAVFVLK
Sbjct: 181 VSIEKHVGLLEEILLVLVCVFPISIEGLSKLGSADSLKCLVSFLMEKNLSPKQNAVFVLK 240

Query: 241 ELLLVDQRYVDSLAAIEGVSEALVSIIREPLCPSATKSSLTAIFYMILPSEISEKMTLKF 300
           ELLLVDQRYVDSLAAIEGVSEALVSIIREPLCPSATKSSLTAIFYMILPSEISEKMTLKF
Sbjct: 241 ELLLVDQRYVDSLAAIEGVSEALVSIIREPLCPSATKSSLTAIFYMILPSEISEKMTLKF 300

Query: 301 MELGLVSQLLDFLVDAEKSLCEKALGVLDGICDFKQGREKLYQNALTIPLLVKKILRVSE 360
           MELGLVSQLLDFLVDAEKSLCEKALGVLDGICDFKQGREKLYQNALTIPLLVKKILRVSE
Sbjct: 301 MELGLVSQLLDFLVDAEKSLCEKALGVLDGICDFKQGREKLYQNALTIPLLVKKILRVSE 360

Query: 361 VASEYCLSILLKLCKSGEKGEDEARVEAAQLGAFQKIIVLLQVGCGGDMKNKVTEMLKLL 420
           VASEYCLSILLKLCKSGEKGEDEARVEAAQLGAFQKIIVLLQVGCGGDMKNKVTEMLKLL
Sbjct: 361 VASEYCLSILLKLCKSGEKGEDEARVEAAQLGAFQKIIVLLQVGCGGDMKNKVTEMLKLL 420

Query: 421 NLYKDRLDCIDSSMHFKFLKKSF 444
           NLYKDRLDCIDSSMHFKFLKKSF
Sbjct: 421 NLYKDRLDCIDSSMHFKFLKKSF 443

BLAST of Carg07457 vs. NCBI nr
Match: XP_023523202.1 (U-box domain-containing protein 21-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 863.6 bits (2230), Expect = 7.4e-247
Identity = 438/443 (98.87%), Postives = 441/443 (99.55%), Query Frame = 0

Query: 1   MFLSWRRSKLGFLPKLKKEHLPGVDSGSEIVIPSHFLCPISLDLMKDPVILSTGITYDRE 60
           MFLSWRRSKLGFLPKLKKEHLPGVDSGSEIVIPSHFLCPISLDLMKDPVILSTGITYDRE
Sbjct: 1   MFLSWRRSKLGFLPKLKKEHLPGVDSGSEIVIPSHFLCPISLDLMKDPVILSTGITYDRE 60

Query: 61  SIEKWIEGGKFSCPVTKQDLMSFDLIPNHTLRRMIQDWCVANHSYGIERIPTPRIPVTPY 120
           SIEKWIEGG FSCPVTKQDLMSFDLIPNHTLRRMIQDWCVANHSYGIERIPTPRIPVTPY
Sbjct: 61  SIEKWIEGGNFSCPVTKQDLMSFDLIPNHTLRRMIQDWCVANHSYGIERIPTPRIPVTPY 120

Query: 121 EVNDVCSRIAVATQRGDSKRCSELVGKIRNWARESERNRRCVVNGGTGIVLAASFEHFSG 180
           EVNDVCSRIAVATQRGDSKRCSELVGKIRNWARESERNRRCVVNGGTGIVLAASFEHF+G
Sbjct: 121 EVNDVCSRIAVATQRGDSKRCSELVGKIRNWARESERNRRCVVNGGTGIVLAASFEHFAG 180

Query: 181 VSIEKHVGLLEEILLVLVCVFPISIEGLSKLGSADSLKCLVSFLMEKNLSPKQNAVFVLK 240
           VSIEKHVGLLEEILLVLVCVFPISIEGLSKLGSADSLKCLVSFLM KNLSPKQNAVFVLK
Sbjct: 181 VSIEKHVGLLEEILLVLVCVFPISIEGLSKLGSADSLKCLVSFLMGKNLSPKQNAVFVLK 240

Query: 241 ELLLVDQRYVDSLAAIEGVSEALVSIIREPLCPSATKSSLTAIFYMILPSEISEKMTLKF 300
           ELLLVDQRYVDSLAAIEGVSEALVSIIREPLCPSATKSSLTAIFYMILPSEISEKMTLKF
Sbjct: 241 ELLLVDQRYVDSLAAIEGVSEALVSIIREPLCPSATKSSLTAIFYMILPSEISEKMTLKF 300

Query: 301 MELGLVSQLLDFLVDAEKSLCEKALGVLDGICDFKQGREKLYQNALTIPLLVKKILRVSE 360
           MELGLVS+LLDFLVDAEKSLCEKALGVLDGICDFKQGREKLYQNALTIPLLVKKILRVSE
Sbjct: 301 MELGLVSELLDFLVDAEKSLCEKALGVLDGICDFKQGREKLYQNALTIPLLVKKILRVSE 360

Query: 361 VASEYCLSILLKLCKSGEKGEDEARVEAAQLGAFQKIIVLLQVGCGGDMKNKVTEMLKLL 420
           VASEYCLSILLKLCKSGEKGEDEARVEAAQLGAFQKIIVLLQVGCGGDMK+KVTEMLKLL
Sbjct: 361 VASEYCLSILLKLCKSGEKGEDEARVEAAQLGAFQKIIVLLQVGCGGDMKDKVTEMLKLL 420

Query: 421 NLYKDRLDCIDSSMHFKFLKKSF 444
           NLYKDRLDCIDSSMHFKFLKKSF
Sbjct: 421 NLYKDRLDCIDSSMHFKFLKKSF 443

BLAST of Carg07457 vs. NCBI nr
Match: XP_022949556.1 (U-box domain-containing protein 21-like [Cucurbita moschata])

HSP 1 Score: 862.4 bits (2227), Expect = 1.6e-246
Identity = 437/443 (98.65%), Postives = 441/443 (99.55%), Query Frame = 0

Query: 1   MFLSWRRSKLGFLPKLKKEHLPGVDSGSEIVIPSHFLCPISLDLMKDPVILSTGITYDRE 60
           MFLSWRRSKLGFLPKLKKEHLPGVDSGSEIVIPSHFLCPISLDLMKDPVILSTGITYDRE
Sbjct: 1   MFLSWRRSKLGFLPKLKKEHLPGVDSGSEIVIPSHFLCPISLDLMKDPVILSTGITYDRE 60

Query: 61  SIEKWIEGGKFSCPVTKQDLMSFDLIPNHTLRRMIQDWCVANHSYGIERIPTPRIPVTPY 120
           SIEKWIEGG FSCPVTKQDLMSFDLIPNHTLRRMIQDWCVANHSYGIERIPTPRIPVTPY
Sbjct: 61  SIEKWIEGGNFSCPVTKQDLMSFDLIPNHTLRRMIQDWCVANHSYGIERIPTPRIPVTPY 120

Query: 121 EVNDVCSRIAVATQRGDSKRCSELVGKIRNWARESERNRRCVVNGGTGIVLAASFEHFSG 180
           EVNDVCSRIAVATQRGDSKRCSELVGKIRNWARESERNRRCVVNGGTGIVLAASFEHF+G
Sbjct: 121 EVNDVCSRIAVATQRGDSKRCSELVGKIRNWARESERNRRCVVNGGTGIVLAASFEHFAG 180

Query: 181 VSIEKHVGLLEEILLVLVCVFPISIEGLSKLGSADSLKCLVSFLMEKNLSPKQNAVFVLK 240
           VSIEKHVGLLEEILLVLVCVFPISIEGLSKLGSADSLKC+VSFLM KNLSPKQNAVFVLK
Sbjct: 181 VSIEKHVGLLEEILLVLVCVFPISIEGLSKLGSADSLKCMVSFLMGKNLSPKQNAVFVLK 240

Query: 241 ELLLVDQRYVDSLAAIEGVSEALVSIIREPLCPSATKSSLTAIFYMILPSEISEKMTLKF 300
           ELLLVDQRYVDSLAAIEGVSEALVSIIREPLCPSATKSSLTAIFY+ILPSEISEKMTLKF
Sbjct: 241 ELLLVDQRYVDSLAAIEGVSEALVSIIREPLCPSATKSSLTAIFYIILPSEISEKMTLKF 300

Query: 301 MELGLVSQLLDFLVDAEKSLCEKALGVLDGICDFKQGREKLYQNALTIPLLVKKILRVSE 360
           MELGLVSQLLDFLVDAEKSLCEKALGVLDGICDFKQGREKLYQNALTIPLLVKKILRVSE
Sbjct: 301 MELGLVSQLLDFLVDAEKSLCEKALGVLDGICDFKQGREKLYQNALTIPLLVKKILRVSE 360

Query: 361 VASEYCLSILLKLCKSGEKGEDEARVEAAQLGAFQKIIVLLQVGCGGDMKNKVTEMLKLL 420
           VASEYCLSILLKLCKSGEKGEDEARVEAAQLGAFQKIIVLLQVGCGGDMK+KVTEMLKLL
Sbjct: 361 VASEYCLSILLKLCKSGEKGEDEARVEAAQLGAFQKIIVLLQVGCGGDMKDKVTEMLKLL 420

Query: 421 NLYKDRLDCIDSSMHFKFLKKSF 444
           NLYKDRLDCIDSSMHFKFLKKSF
Sbjct: 421 NLYKDRLDCIDSSMHFKFLKKSF 443

BLAST of Carg07457 vs. NCBI nr
Match: XP_022997974.1 (U-box domain-containing protein 21-like [Cucurbita maxima])

HSP 1 Score: 861.3 bits (2224), Expect = 3.7e-246
Identity = 436/443 (98.42%), Postives = 441/443 (99.55%), Query Frame = 0

Query: 1   MFLSWRRSKLGFLPKLKKEHLPGVDSGSEIVIPSHFLCPISLDLMKDPVILSTGITYDRE 60
           MFLSWR+SKLGFLPKLKKEHLPGVDSGSEIVIPSHFLCPISLDLMKDPVILSTGITYDRE
Sbjct: 1   MFLSWRKSKLGFLPKLKKEHLPGVDSGSEIVIPSHFLCPISLDLMKDPVILSTGITYDRE 60

Query: 61  SIEKWIEGGKFSCPVTKQDLMSFDLIPNHTLRRMIQDWCVANHSYGIERIPTPRIPVTPY 120
           SIEKWIEGG FSCPVTKQDLMSFDLIPNHTLRRMIQDWCVANHSYGIERIPTPRIPVTPY
Sbjct: 61  SIEKWIEGGNFSCPVTKQDLMSFDLIPNHTLRRMIQDWCVANHSYGIERIPTPRIPVTPY 120

Query: 121 EVNDVCSRIAVATQRGDSKRCSELVGKIRNWARESERNRRCVVNGGTGIVLAASFEHFSG 180
           EVNDVCSRIAVATQRGDSKRCSELVGKIRNWARESERNRRCVVNGGTGIVLAASFEHF+G
Sbjct: 121 EVNDVCSRIAVATQRGDSKRCSELVGKIRNWARESERNRRCVVNGGTGIVLAASFEHFAG 180

Query: 181 VSIEKHVGLLEEILLVLVCVFPISIEGLSKLGSADSLKCLVSFLMEKNLSPKQNAVFVLK 240
           VSIEKHVGLLEEILLVLVCVFPISIEGLSKLGSADSLKCLVSFL+ KNLSPKQNAVFVLK
Sbjct: 181 VSIEKHVGLLEEILLVLVCVFPISIEGLSKLGSADSLKCLVSFLVGKNLSPKQNAVFVLK 240

Query: 241 ELLLVDQRYVDSLAAIEGVSEALVSIIREPLCPSATKSSLTAIFYMILPSEISEKMTLKF 300
           ELLLVDQRYVDSLAAIEGVSEALVSIIREPLCPSATKSSLTAIFYMILPSEISEKMT+KF
Sbjct: 241 ELLLVDQRYVDSLAAIEGVSEALVSIIREPLCPSATKSSLTAIFYMILPSEISEKMTMKF 300

Query: 301 MELGLVSQLLDFLVDAEKSLCEKALGVLDGICDFKQGREKLYQNALTIPLLVKKILRVSE 360
           MELGLVSQLLDFLVDAEKSLCEKALGVLDGICDFKQGREKLYQNALTIPLLVKKILRVSE
Sbjct: 301 MELGLVSQLLDFLVDAEKSLCEKALGVLDGICDFKQGREKLYQNALTIPLLVKKILRVSE 360

Query: 361 VASEYCLSILLKLCKSGEKGEDEARVEAAQLGAFQKIIVLLQVGCGGDMKNKVTEMLKLL 420
           VASEYCLSILLKLCKSGEKGEDEARVEAAQLGAFQKIIVLLQVGCGGDMK+KVTEMLKLL
Sbjct: 361 VASEYCLSILLKLCKSGEKGEDEARVEAAQLGAFQKIIVLLQVGCGGDMKDKVTEMLKLL 420

Query: 421 NLYKDRLDCIDSSMHFKFLKKSF 444
           NLYKDRLDCIDSSMHFKFLKKSF
Sbjct: 421 NLYKDRLDCIDSSMHFKFLKKSF 443

BLAST of Carg07457 vs. NCBI nr
Match: XP_038899398.1 (U-box domain-containing protein 21 [Benincasa hispida])

HSP 1 Score: 777.3 bits (2006), Expect = 6.9e-221
Identity = 384/443 (86.68%), Postives = 422/443 (95.26%), Query Frame = 0

Query: 1   MFLSWRRSKLGFLPKLKKEHLPGVDSGSEIVIPSHFLCPISLDLMKDPVILSTGITYDRE 60
           M  SWR++KLGFL KLKKE LPGVDSGSEI IPSH++CPISLDLMKDPVILSTGITYDRE
Sbjct: 1   MIFSWRKNKLGFLTKLKKE-LPGVDSGSEIAIPSHYMCPISLDLMKDPVILSTGITYDRE 60

Query: 61  SIEKWIEGGKFSCPVTKQDLMSFDLIPNHTLRRMIQDWCVANHSYGIERIPTPRIPVTPY 120
           SIEKWI+GG FSCPVTKQDL+SFDLIPNH+LRR+IQ WCVAN SYGIERIPTPRIPV+PY
Sbjct: 61  SIEKWIDGGNFSCPVTKQDLISFDLIPNHSLRRLIQGWCVANRSYGIERIPTPRIPVSPY 120

Query: 121 EVNDVCSRIAVATQRGDSKRCSELVGKIRNWARESERNRRCVVNGGTGIVLAASFEHFSG 180
           EV ++CSRI++ATQR DSK+CSEL+GKIRNWA+ESERNRRC+VNGGTG VLAASFEHF+G
Sbjct: 121 EVKEICSRISIATQRSDSKKCSELMGKIRNWAKESERNRRCIVNGGTGDVLAASFEHFAG 180

Query: 181 VSIEKHVGLLEEILLVLVCVFPISIEGLSKLGSADSLKCLVSFLMEKNLSPKQNAVFVLK 240
           VSIEKHVGLLEEILLVL CVFP+++EGLSKLGSADSLKCLVSFL+ K+LSPKQNAVFVLK
Sbjct: 181 VSIEKHVGLLEEILLVLACVFPVAVEGLSKLGSADSLKCLVSFLIGKDLSPKQNAVFVLK 240

Query: 241 ELLLVDQRYVDSLAAIEGVSEALVSIIREPLCPSATKSSLTAIFYMILPSEISEKMTLKF 300
           ELL  D+RYV+SLAAIEGVSEALVSII+EPLCPSATKSSLT IFYMILPS+I EKM LKF
Sbjct: 241 ELLSADRRYVNSLAAIEGVSEALVSIIKEPLCPSATKSSLTVIFYMILPSDIGEKMALKF 300

Query: 301 MELGLVSQLLDFLVDAEKSLCEKALGVLDGICDFKQGREKLYQNALTIPLLVKKILRVSE 360
           +ELGLVSQLL+FLVDAEKSLCEKALG+LDG+CD+KQGREKLY+NALTIPLLVKKILRVSE
Sbjct: 301 VELGLVSQLLEFLVDAEKSLCEKALGILDGVCDYKQGREKLYKNALTIPLLVKKILRVSE 360

Query: 361 VASEYCLSILLKLCKSGEKGEDEARVEAAQLGAFQKIIVLLQVGCGGDMKNKVTEMLKLL 420
           +A+EY LSILLKLCKSGEKGEDE RVEA QLGAFQKI+VLLQVGCGGDMK+KVTEMLKLL
Sbjct: 361 LATEYSLSILLKLCKSGEKGEDEVRVEAVQLGAFQKILVLLQVGCGGDMKDKVTEMLKLL 420

Query: 421 NLYKDRLDCIDSSMHFKFLKKSF 444
           NLYKDRLDCIDSSMHFK+LKKSF
Sbjct: 421 NLYKDRLDCIDSSMHFKYLKKSF 442

BLAST of Carg07457 vs. ExPASy Swiss-Prot
Match: Q5PNY6 (U-box domain-containing protein 21 OS=Arabidopsis thaliana OX=3702 GN=PUB21 PE=2 SV=1)

HSP 1 Score: 395.2 bits (1014), Expect = 9.7e-109
Identity = 210/423 (49.65%), Postives = 290/423 (68.56%), Query Frame = 0

Query: 22  PGVDSGSEIVIPSHFLCPISLDLMKDPVILSTGITYDRESIEKWIEGGKFSCPVTKQDLM 81
           P  +  SEI IP  F CPIS+DLMKDPVI+STGITYDR SIE WI  G  +CPVT   L 
Sbjct: 21  PESEPESEITIPPEFQCPISIDLMKDPVIISTGITYDRVSIETWINSGNKTCPVTNTVLT 80

Query: 82  SFDLIPNHTLRRMIQDWCVANHSYGIERIPTPRIPVTPYEVNDVCSRIAVATQRGDSKRC 141
           +FD IPNHT+R+MIQ WCV   S  I+RIPTPR+P+ P EV ++  +++ AT+RGD ++C
Sbjct: 81  TFDQIPNHTIRKMIQGWCVEKGSPLIQRIPTPRVPLMPCEVYEISRKLSSATRRGDYEKC 140

Query: 142 SELVGKIRNWARESERNRRCVVNGGTGIVLAASFEHFSGVSIEKHVGLLEEILLVLVCVF 201
             ++ KI+    ESE+NR+CV     G VL   F+ FSG   EK   +L EIL +L  +F
Sbjct: 141 GVIIEKIKKLGDESEKNRKCVNENSVGWVLCDCFDKFSG--DEKLTFMLNEILSLLTWMF 200

Query: 202 PISIEGLSKLGSADSLKCLVSFLMEKNLSPKQNAVFVLKELLLVDQRYVDSLAAIEGVSE 261
           PI +EG+SKL SA S +C+   L   + S +QNA F++KE+L +D+  V S A   GV+E
Sbjct: 201 PIGLEGISKLASATSFRCVAGLLKSTDDSVRQNAAFIMKEILSLDETRVHSFAVENGVAE 260

Query: 262 ALVSIIREPLCPSATKSSLTAIFYMILPSEISEKMTLKFMELGLVSQLLDFLVDAEKSLC 321
           ALV +IR+ +  S+TKSSL AI+ M+L      ++  +F+E+GLVS  ++ +VDAE S+C
Sbjct: 261 ALVKLIRDSVSSSSTKSSLIAIYQMVLQ---KPEIASEFLEIGLVSITVEMIVDAENSVC 320

Query: 322 EKALGVLDGICDFKQGREKLYQNALTIPLLVKKILRVSEVASEYCLSILLKLCKSGEKGE 381
           EKAL VLD IC+ + GRE++ +NAL +PLLVKKI +VSE+A+   +S++LKL K+G    
Sbjct: 321 EKALAVLDAICETEHGREEVRKNALVMPLLVKKIAKVSELATRSSMSMILKLWKTGNTVA 380

Query: 382 DEARVEAAQLGAFQKIIVLLQVGCGGDMKNKVTEMLKLLNL-YKDRLDCIDSSMHFKFLK 441
            E   +A +LGAFQK++++LQVG G + K K TE+LK++N   K   DC+DS   FK++K
Sbjct: 381 VE---DAVRLGAFQKVLLVLQVGYGEETKEKATELLKMMNTQMKLMSDCVDSLKEFKYIK 435

Query: 442 KSF 444
           K F
Sbjct: 441 KPF 435

BLAST of Carg07457 vs. ExPASy Swiss-Prot
Match: Q9C8D1 (U-box domain-containing protein 20 OS=Arabidopsis thaliana OX=3702 GN=PUB20 PE=1 SV=1)

HSP 1 Score: 359.8 bits (922), Expect = 4.5e-98
Identity = 207/434 (47.70%), Postives = 285/434 (65.67%), Query Frame = 0

Query: 17  KKEHLPGVDSGS--EIVIPSHFLCPISLDLMKDPVILSTGITYDRESIEKWIEGGKFSCP 76
           KKE +P        EI IPS F CPIS +LMKDPVI+++GITYDRE+IEKW E G  +CP
Sbjct: 16  KKEIIPVTSCSEEVEITIPSQFQCPISYELMKDPVIIASGITYDRENIEKWFESGYQTCP 75

Query: 77  VTKQDLMSFDLIPNHTLRRMIQDWCVANHSYGIERIPTPRIPVTPYEVNDVCSRIAVATQ 136
           VT   L S + IPNHT+RRMIQ WC ++   GIERIPTPR+PVT ++V+++C R++ AT+
Sbjct: 76  VTNTVLTSLEQIPNHTIRRMIQGWCGSSLGGGIERIPTPRVPVTSHQVSEICERLSAATR 135

Query: 137 RGDSKRCSELVGKIRNWARESERNRRCVVNGGTGIVLAASFEHFSGVSIEKHVGLLEEIL 196
           RGD   C E+V K+    +ESERNR+CV   G G+VL   F+ FS  +      LLEE +
Sbjct: 136 RGDYAACMEMVTKMTRLGKESERNRKCVKENGAGLVLCVCFDAFSENANASL--LLEETV 195

Query: 197 LVLVCVFPISIEGLSKLGSADSLKCLVSFLMEKNLSPKQNAVFVLKELLLVDQRYVDSLA 256
            VL  + PI +EG SKL +  S   LV  L   +    QNA F++KELL ++  +V +L 
Sbjct: 196 SVLTWMLPIGLEGQSKLTTTSSFNRLVELLRNGD----QNAAFLIKELLELNVTHVHALT 255

Query: 257 AIEGVSEALV-SIIREPLCPSATKSSLTAIFYMILPSEISEKMTLKFMELGLVSQLLDFL 316
            I GV EA + SI R+  C     +SL +I +MIL    +++   +F+EL LV+  ++ L
Sbjct: 256 KINGVQEAFMKSINRDSTC----VNSLISIHHMIL---TNQETVSRFLELDLVNITVEML 315

Query: 317 VDAEKSLCEKALGVLDGICDFKQGREKLYQNALTIPLLVKKILRVSEVASEYCLSILLKL 376
           VD+E S+CEKAL VL+ IC+ K+GREK+ +N L IP+LVKKIL++SE   +  +S++ K+
Sbjct: 316 VDSENSVCEKALTVLNVICETKEGREKVRRNKLVIPILVKKILKISE--KKDLVSVMWKV 375

Query: 377 CKSGEKGEDEARVEAAQLGAFQKIIVLLQVGCGGDMKNKVTEMLKLLNLYKDRLDCID-- 436
           CKSG+  E E   EA +LGAF+K++V+LQVGCG   K KVTE+LK++N        +D  
Sbjct: 376 CKSGDGSEVE---EALRLGAFKKLVVMLQVGCGEGTKEKVTELLKMMNKVMKMNGFVDRS 431

Query: 437 --SSMHFKFLKKSF 444
             SS+ FK +KK F
Sbjct: 436 YSSSIEFKHVKKPF 431

BLAST of Carg07457 vs. ExPASy Swiss-Prot
Match: Q9FXA4 (U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 1.5e-53
Identity = 141/398 (35.43%), Postives = 210/398 (52.76%), Query Frame = 0

Query: 30  IVIPSHFLCPISLDLMKDPVILSTGITYDRESIEKWIEGGKFSCPVTKQDLMSFDLIPNH 89
           I IP HF CPISLDLM DPV +STG TYDR SI+ WI  G  +CPVT+  L  F LIPNH
Sbjct: 12  IQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGNTTCPVTRVALSDFTLIPNH 71

Query: 90  TLRRMIQDWCVANHSYGIERIPTPRIPVTPYEVNDVCSRIAVATQRGDSKRC-SELVGKI 149
           TLRR+IQ+WCVAN S G+ERIPTP+ P  P  V  + S+ +  T    S R  +  + ++
Sbjct: 72  TLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRAAAIRRL 131

Query: 150 RNWARESERNRRCVV-NGGTGIVLAASFEHFSGVSIEKHVGLLEEILLVLVCVFPISIEG 209
           R  AR+SE+NR  +  +    I++   F      S+     L+ E L +LV +     E 
Sbjct: 132 RGLARDSEKNRVLIAGHNAREILVRILFADIETTSLSSE--LVSESLALLVLLHMTETEC 191

Query: 210 LSKLGSADSLKCLVSFLMEKNLSPKQNAVFVLKELLLVDQRYVDSLAAIEG---VSEALV 269
            +       +  +   L + ++  + NA   L E++L   + +D    I G   + E ++
Sbjct: 192 EAVASDPSRVGFMTRLLFDSSIEIRVNAA-ALIEMVLTGAKSMDLKLIISGSDSIFEGVL 251

Query: 270 SIIREPLCP-SATKSSLTAIFYMILPSEISEKMTLKFMELGLVSQLLDFL-VDAEKSLCE 329
            +++ P+    A K  + AIF + L  +         +  G    L+D L  D ++   E
Sbjct: 252 DLLKNPISSRRALKIGIKAIFALCLVKQTRHLA----ISAGAPGILIDRLAADFDRCDTE 311

Query: 330 KALGVLDGICDFKQGREKLYQNALTIPLLVKKILRVSEVASEYCLSILLKLCKSGEKGED 389
           + L  ++ +C   +G     ++ALT+PL+VK ILRVS+ A+EY    LL LC +    E+
Sbjct: 312 RGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGALLALCTA----EE 371

Query: 390 EARVEAAQLGAFQKIIVLLQVGCGGDMKNKVTEMLKLL 421
             R EAA  G   ++++L+Q  C    K K   +LKLL
Sbjct: 372 RCRDEAAAAGLVTQLLLLVQSDCTERAKRKAQMLLKLL 398

BLAST of Carg07457 vs. ExPASy Swiss-Prot
Match: Q84TG3 (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 207.2 bits (526), Expect = 3.8e-52
Identity = 152/417 (36.45%), Postives = 222/417 (53.24%), Query Frame = 0

Query: 29  EIVIPSHFLCPISLDLMKDPVILSTGITYDRESIEKWI-EGGKFSCPVTKQDLMSFDLIP 88
           EI IP  FLCPISL++MKDPVI+STGITYDR+SIEKW+  G K SCPVTKQD+   DL P
Sbjct: 9   EIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDADLTP 68

Query: 89  NHTLRRMIQDWCVANHSYGIERIPTPRIPVTPYEVNDVCSRIAVATQRGDSKRCSELVGK 148
           NHTLRR+IQ WC  N SYG+ERIPTPR P+   E+  +      A+   +  +C   + +
Sbjct: 69  NHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIR--DSASSHENQVKC---LKR 128

Query: 149 IRNWARESERNRRCVVNGGTGIVLAASFEHFSGVSIEKHVGLLEEILLVLVCVFPISIEG 208
           +R    E+  N+RC+   G    LA      + VS +   G L +  L L+     S   
Sbjct: 129 LRQIVSENATNKRCLEAAGVPEFLA------NIVSNDSENGSLTDEALNLLYHLETSETV 188

Query: 209 LSKL----GSADSLKCLVSFLMEKNLSPKQNAVFVLKELLLVDQRYVDSLAAIEGVSEAL 268
           L  L       + +K L   +       +  A  +LK +L V    + S+     V   +
Sbjct: 189 LKNLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADP-MQSMTLKPEVFTEV 248

Query: 269 VSIIREPLCPSATKSSLTAIFYMILPSEISEKMTLKFMELGLVSQLLDFLVD----AEKS 328
           V I+ + +   ATK+++  I   I P   +     K +E G++S +++ L+D    +E+ 
Sbjct: 249 VQILDDRISQKATKAAM-HILVNICPWGRNRH---KAVEAGVISVIIELLMDESFTSERR 308

Query: 329 LCEKALGVLDGICDFKQGREKLYQNALTIPLLVKKILRVSEVASEYCLSILLKLCKSGEK 388
             E A+ VLD +C   +GR +   +   I ++ KKILRVS+ AS+  + +LL +   G  
Sbjct: 309 GPEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSV---GRF 368

Query: 389 GEDEARV-EAAQLGAFQKIIVLLQVGCGGDMKNKVTEMLKL-LNLYKDRLDCIDSSM 435
               A + E  QLG   K+ ++LQV CGG  K K  E+LKL   ++KD   C+  +M
Sbjct: 369 CATPALLHEMLQLGVVAKLCLVLQVSCGGKTKEKAKELLKLHARVWKDS-PCLPKNM 405

BLAST of Carg07457 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 199.9 bits (507), Expect = 6.0e-50
Identity = 144/402 (35.82%), Postives = 218/402 (54.23%), Query Frame = 0

Query: 30  IVIPSHFLCPISLDLMKDPVILSTGITYDRESIEKWIE-GGKFSCPVTKQDLMSFDLIPN 89
           I IP HF CPISL+LM+DPV + TG TYDR SIE W+  G   +CPVT+  L  F LIPN
Sbjct: 12  IQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDFTLIPN 71

Query: 90  HTLRRMIQDWCVANHSYGIERIPTPRIPVTPYEVNDVCSRIAVATQRGDSKRC-SELVGK 149
           HTLRR+IQ+WCVAN S G+ERIPTP+ P  P  V  + S+ +  T    S R  +  + +
Sbjct: 72  HTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAAALRR 131

Query: 150 IRNWARESERNRRCV-VNGGTGIVLAASFEHFSGVSIEKHVGLLEEILLVLVCVFPISIE 209
           +R +AR+S++NR  +  +  T I++   F   +         L+ E L +LV + PI+  
Sbjct: 132 LRGFARDSDKNRVLIAAHNATEILIKILFSETTSSE------LVSESLALLV-MLPITEP 191

Query: 210 GLSKLGSAD--SLKCLVSFLMEKNLSPKQNAVFVLKELLLVDQRYVD---SLAAIEGVSE 269
                 S+D   ++ L   L + ++  + NA   L E++    +  D   S++  E V E
Sbjct: 192 NQFVSISSDPGRVEFLTRLLFDSSIETRVNAA-ALIEIVSTGTKSADLKGSISNSESVFE 251

Query: 270 ALVSIIREPLCP-SATKSSLTAIFYMILPSEISEKMTLKF-MELGLVSQLLDFL-VDAEK 329
            ++ ++R P+    A K  +  +F +      S K T    +  G    L+D L  D ++
Sbjct: 252 GVLDLLRNPISSRRALKIGIKTLFAL-----CSVKSTRHIAITAGAPEILIDRLAADFDR 311

Query: 330 SLCEKALGVLDGICDFKQGREKLYQNALTIPLLVKKILRVSEVASEYCLSILLKLCKSGE 389
              E+AL  ++ +C   +G     ++ALT+PLLVK ILRVS+ A+EY    LL LC +  
Sbjct: 312 CDTERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLALCTA-- 371

Query: 390 KGEDEARVEAAQLGAFQKIIVLLQVGCGGDMKNKVTEMLKLL 421
             E+  R EAA  G   ++++++Q  C    K K  ++LKLL
Sbjct: 372 --EERWREEAAGAGVVVQLLLMVQSECTERAKKKAQKLLKLL 396

BLAST of Carg07457 vs. ExPASy TrEMBL
Match: A0A6J1GCB6 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111452870 PE=4 SV=1)

HSP 1 Score: 862.4 bits (2227), Expect = 8.0e-247
Identity = 437/443 (98.65%), Postives = 441/443 (99.55%), Query Frame = 0

Query: 1   MFLSWRRSKLGFLPKLKKEHLPGVDSGSEIVIPSHFLCPISLDLMKDPVILSTGITYDRE 60
           MFLSWRRSKLGFLPKLKKEHLPGVDSGSEIVIPSHFLCPISLDLMKDPVILSTGITYDRE
Sbjct: 1   MFLSWRRSKLGFLPKLKKEHLPGVDSGSEIVIPSHFLCPISLDLMKDPVILSTGITYDRE 60

Query: 61  SIEKWIEGGKFSCPVTKQDLMSFDLIPNHTLRRMIQDWCVANHSYGIERIPTPRIPVTPY 120
           SIEKWIEGG FSCPVTKQDLMSFDLIPNHTLRRMIQDWCVANHSYGIERIPTPRIPVTPY
Sbjct: 61  SIEKWIEGGNFSCPVTKQDLMSFDLIPNHTLRRMIQDWCVANHSYGIERIPTPRIPVTPY 120

Query: 121 EVNDVCSRIAVATQRGDSKRCSELVGKIRNWARESERNRRCVVNGGTGIVLAASFEHFSG 180
           EVNDVCSRIAVATQRGDSKRCSELVGKIRNWARESERNRRCVVNGGTGIVLAASFEHF+G
Sbjct: 121 EVNDVCSRIAVATQRGDSKRCSELVGKIRNWARESERNRRCVVNGGTGIVLAASFEHFAG 180

Query: 181 VSIEKHVGLLEEILLVLVCVFPISIEGLSKLGSADSLKCLVSFLMEKNLSPKQNAVFVLK 240
           VSIEKHVGLLEEILLVLVCVFPISIEGLSKLGSADSLKC+VSFLM KNLSPKQNAVFVLK
Sbjct: 181 VSIEKHVGLLEEILLVLVCVFPISIEGLSKLGSADSLKCMVSFLMGKNLSPKQNAVFVLK 240

Query: 241 ELLLVDQRYVDSLAAIEGVSEALVSIIREPLCPSATKSSLTAIFYMILPSEISEKMTLKF 300
           ELLLVDQRYVDSLAAIEGVSEALVSIIREPLCPSATKSSLTAIFY+ILPSEISEKMTLKF
Sbjct: 241 ELLLVDQRYVDSLAAIEGVSEALVSIIREPLCPSATKSSLTAIFYIILPSEISEKMTLKF 300

Query: 301 MELGLVSQLLDFLVDAEKSLCEKALGVLDGICDFKQGREKLYQNALTIPLLVKKILRVSE 360
           MELGLVSQLLDFLVDAEKSLCEKALGVLDGICDFKQGREKLYQNALTIPLLVKKILRVSE
Sbjct: 301 MELGLVSQLLDFLVDAEKSLCEKALGVLDGICDFKQGREKLYQNALTIPLLVKKILRVSE 360

Query: 361 VASEYCLSILLKLCKSGEKGEDEARVEAAQLGAFQKIIVLLQVGCGGDMKNKVTEMLKLL 420
           VASEYCLSILLKLCKSGEKGEDEARVEAAQLGAFQKIIVLLQVGCGGDMK+KVTEMLKLL
Sbjct: 361 VASEYCLSILLKLCKSGEKGEDEARVEAAQLGAFQKIIVLLQVGCGGDMKDKVTEMLKLL 420

Query: 421 NLYKDRLDCIDSSMHFKFLKKSF 444
           NLYKDRLDCIDSSMHFKFLKKSF
Sbjct: 421 NLYKDRLDCIDSSMHFKFLKKSF 443

BLAST of Carg07457 vs. ExPASy TrEMBL
Match: A0A6J1KD23 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111492767 PE=4 SV=1)

HSP 1 Score: 861.3 bits (2224), Expect = 1.8e-246
Identity = 436/443 (98.42%), Postives = 441/443 (99.55%), Query Frame = 0

Query: 1   MFLSWRRSKLGFLPKLKKEHLPGVDSGSEIVIPSHFLCPISLDLMKDPVILSTGITYDRE 60
           MFLSWR+SKLGFLPKLKKEHLPGVDSGSEIVIPSHFLCPISLDLMKDPVILSTGITYDRE
Sbjct: 1   MFLSWRKSKLGFLPKLKKEHLPGVDSGSEIVIPSHFLCPISLDLMKDPVILSTGITYDRE 60

Query: 61  SIEKWIEGGKFSCPVTKQDLMSFDLIPNHTLRRMIQDWCVANHSYGIERIPTPRIPVTPY 120
           SIEKWIEGG FSCPVTKQDLMSFDLIPNHTLRRMIQDWCVANHSYGIERIPTPRIPVTPY
Sbjct: 61  SIEKWIEGGNFSCPVTKQDLMSFDLIPNHTLRRMIQDWCVANHSYGIERIPTPRIPVTPY 120

Query: 121 EVNDVCSRIAVATQRGDSKRCSELVGKIRNWARESERNRRCVVNGGTGIVLAASFEHFSG 180
           EVNDVCSRIAVATQRGDSKRCSELVGKIRNWARESERNRRCVVNGGTGIVLAASFEHF+G
Sbjct: 121 EVNDVCSRIAVATQRGDSKRCSELVGKIRNWARESERNRRCVVNGGTGIVLAASFEHFAG 180

Query: 181 VSIEKHVGLLEEILLVLVCVFPISIEGLSKLGSADSLKCLVSFLMEKNLSPKQNAVFVLK 240
           VSIEKHVGLLEEILLVLVCVFPISIEGLSKLGSADSLKCLVSFL+ KNLSPKQNAVFVLK
Sbjct: 181 VSIEKHVGLLEEILLVLVCVFPISIEGLSKLGSADSLKCLVSFLVGKNLSPKQNAVFVLK 240

Query: 241 ELLLVDQRYVDSLAAIEGVSEALVSIIREPLCPSATKSSLTAIFYMILPSEISEKMTLKF 300
           ELLLVDQRYVDSLAAIEGVSEALVSIIREPLCPSATKSSLTAIFYMILPSEISEKMT+KF
Sbjct: 241 ELLLVDQRYVDSLAAIEGVSEALVSIIREPLCPSATKSSLTAIFYMILPSEISEKMTMKF 300

Query: 301 MELGLVSQLLDFLVDAEKSLCEKALGVLDGICDFKQGREKLYQNALTIPLLVKKILRVSE 360
           MELGLVSQLLDFLVDAEKSLCEKALGVLDGICDFKQGREKLYQNALTIPLLVKKILRVSE
Sbjct: 301 MELGLVSQLLDFLVDAEKSLCEKALGVLDGICDFKQGREKLYQNALTIPLLVKKILRVSE 360

Query: 361 VASEYCLSILLKLCKSGEKGEDEARVEAAQLGAFQKIIVLLQVGCGGDMKNKVTEMLKLL 420
           VASEYCLSILLKLCKSGEKGEDEARVEAAQLGAFQKIIVLLQVGCGGDMK+KVTEMLKLL
Sbjct: 361 VASEYCLSILLKLCKSGEKGEDEARVEAAQLGAFQKIIVLLQVGCGGDMKDKVTEMLKLL 420

Query: 421 NLYKDRLDCIDSSMHFKFLKKSF 444
           NLYKDRLDCIDSSMHFKFLKKSF
Sbjct: 421 NLYKDRLDCIDSSMHFKFLKKSF 443

BLAST of Carg07457 vs. ExPASy TrEMBL
Match: A0A0A0LAV4 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G308190 PE=4 SV=1)

HSP 1 Score: 776.2 bits (2003), Expect = 7.5e-221
Identity = 385/443 (86.91%), Postives = 421/443 (95.03%), Query Frame = 0

Query: 1   MFLSWRRSKLGFLPKLKKEHLPGVDSGSEIVIPSHFLCPISLDLMKDPVILSTGITYDRE 60
           M  SWR++KLGFL KLKKE LPGVDSGSEI IPSH++CPISLDLMKDPVILSTGITYDRE
Sbjct: 1   MIFSWRKNKLGFLTKLKKE-LPGVDSGSEIAIPSHYMCPISLDLMKDPVILSTGITYDRE 60

Query: 61  SIEKWIEGGKFSCPVTKQDLMSFDLIPNHTLRRMIQDWCVANHSYGIERIPTPRIPVTPY 120
           SIEKWI+GG FSCPVTKQDL  FDLIPNH LRR+IQDWCVAN SYGIERIPTPRIPV+PY
Sbjct: 61  SIEKWIDGGNFSCPVTKQDLTVFDLIPNHALRRLIQDWCVANRSYGIERIPTPRIPVSPY 120

Query: 121 EVNDVCSRIAVATQRGDSKRCSELVGKIRNWARESERNRRCVVNGGTGIVLAASFEHFSG 180
           EV ++CSRI++ATQR DSKRCSEL+GKIRNWA+ESERNRRC+VNGGTG VLAASFEHF+G
Sbjct: 121 EVKEICSRISIATQRSDSKRCSELMGKIRNWAKESERNRRCIVNGGTGDVLAASFEHFAG 180

Query: 181 VSIEKHVGLLEEILLVLVCVFPISIEGLSKLGSADSLKCLVSFLMEKNLSPKQNAVFVLK 240
           VSIEKHVGLLEEILLVL CV+P++IEGLSKLGSADSLKCLVSFL+ K+LSPKQ+A+FVLK
Sbjct: 181 VSIEKHVGLLEEILLVLTCVYPVAIEGLSKLGSADSLKCLVSFLVGKDLSPKQSAIFVLK 240

Query: 241 ELLLVDQRYVDSLAAIEGVSEALVSIIREPLCPSATKSSLTAIFYMILPSEISEKMTLKF 300
           ELL  D+RYV+SLAAIEGVSEALVSIIR+PLCPSATKSSLTAIFYMILPS+I EKM LKF
Sbjct: 241 ELLAADRRYVNSLAAIEGVSEALVSIIRDPLCPSATKSSLTAIFYMILPSDIGEKMALKF 300

Query: 301 MELGLVSQLLDFLVDAEKSLCEKALGVLDGICDFKQGREKLYQNALTIPLLVKKILRVSE 360
           +ELGLVSQLL+FLVDAEKSLCEKALG+LDGICD+KQGREKLY NALTIPLLVKKILRVSE
Sbjct: 301 VELGLVSQLLEFLVDAEKSLCEKALGILDGICDYKQGREKLYNNALTIPLLVKKILRVSE 360

Query: 361 VASEYCLSILLKLCKSGEKGEDEARVEAAQLGAFQKIIVLLQVGCGGDMKNKVTEMLKLL 420
           +A+EY LSILLKLCKSGEKGE+E RVEAAQLGAFQKI+VLLQVGCGGDMK+KVTEMLKLL
Sbjct: 361 LATEYSLSILLKLCKSGEKGENEVRVEAAQLGAFQKILVLLQVGCGGDMKDKVTEMLKLL 420

Query: 421 NLYKDRLDCIDSSMHFKFLKKSF 444
           NLYKDRLDCIDSSMHFK+LKKSF
Sbjct: 421 NLYKDRLDCIDSSMHFKYLKKSF 442

BLAST of Carg07457 vs. ExPASy TrEMBL
Match: A0A5D3BS09 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold451G001510 PE=4 SV=1)

HSP 1 Score: 764.2 bits (1972), Expect = 2.9e-217
Identity = 379/443 (85.55%), Postives = 416/443 (93.91%), Query Frame = 0

Query: 1   MFLSWRRSKLGFLPKLKKEHLPGVDSGSEIVIPSHFLCPISLDLMKDPVILSTGITYDRE 60
           M  SWR++KLGFL KLKK+ LPGVD GSEI IPSH++CPISLDLMKDPVILSTGITYDRE
Sbjct: 1   MIFSWRKNKLGFLTKLKKQ-LPGVDCGSEIAIPSHYMCPISLDLMKDPVILSTGITYDRE 60

Query: 61  SIEKWIEGGKFSCPVTKQDLMSFDLIPNHTLRRMIQDWCVANHSYGIERIPTPRIPVTPY 120
           SIEKWI+GG FSCPVTKQ+L  FDLIPNH LRR+IQDWCVAN SYGIERIPTPRIPV+PY
Sbjct: 61  SIEKWIDGGNFSCPVTKQELTVFDLIPNHALRRLIQDWCVANRSYGIERIPTPRIPVSPY 120

Query: 121 EVNDVCSRIAVATQRGDSKRCSELVGKIRNWARESERNRRCVVNGGTGIVLAASFEHFSG 180
           EV ++CSRI++ATQR D KRCSEL+GKIRNWA+ESERNRRC+VNGGTG VLAASFEHF+G
Sbjct: 121 EVKEICSRISIATQRSDLKRCSELMGKIRNWAKESERNRRCLVNGGTGDVLAASFEHFAG 180

Query: 181 VSIEKHVGLLEEILLVLVCVFPISIEGLSKLGSADSLKCLVSFLMEKNLSPKQNAVFVLK 240
           VSIEKHVGLLEEILLVL C+FP++IEG+SKLGSA SLKCLVSFL+ K+LSPKQ+AVFVLK
Sbjct: 181 VSIEKHVGLLEEILLVLTCIFPVAIEGISKLGSAGSLKCLVSFLIGKDLSPKQSAVFVLK 240

Query: 241 ELLLVDQRYVDSLAAIEGVSEALVSIIREPLCPSATKSSLTAIFYMILPSEISEKMTLKF 300
           ELL  D+RYV+SLAAIEGVSEALVSIIR+PLCPS TKSSLT IFYMILPS+I EKM LKF
Sbjct: 241 ELLAADRRYVNSLAAIEGVSEALVSIIRDPLCPSNTKSSLTVIFYMILPSDIGEKMALKF 300

Query: 301 MELGLVSQLLDFLVDAEKSLCEKALGVLDGICDFKQGREKLYQNALTIPLLVKKILRVSE 360
           +ELGLVSQLL+FLVDAEKSLCEKALG+LDGICD+KQGREKLY NALTIPLLVKKILRVSE
Sbjct: 301 VELGLVSQLLEFLVDAEKSLCEKALGILDGICDYKQGREKLYNNALTIPLLVKKILRVSE 360

Query: 361 VASEYCLSILLKLCKSGEKGEDEARVEAAQLGAFQKIIVLLQVGCGGDMKNKVTEMLKLL 420
           +A+EY LSILLKLCKSGEKGEDE RVEAAQLGAFQKI+VLLQVGCGGDMK+KVTEMLKLL
Sbjct: 361 LATEYSLSILLKLCKSGEKGEDEVRVEAAQLGAFQKILVLLQVGCGGDMKDKVTEMLKLL 420

Query: 421 NLYKDRLDCIDSSMHFKFLKKSF 444
           NLYKDRLDCIDSSMHFK+LKKSF
Sbjct: 421 NLYKDRLDCIDSSMHFKYLKKSF 442

BLAST of Carg07457 vs. ExPASy TrEMBL
Match: A0A1S3C462 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103496814 PE=4 SV=1)

HSP 1 Score: 764.2 bits (1972), Expect = 2.9e-217
Identity = 379/443 (85.55%), Postives = 416/443 (93.91%), Query Frame = 0

Query: 1   MFLSWRRSKLGFLPKLKKEHLPGVDSGSEIVIPSHFLCPISLDLMKDPVILSTGITYDRE 60
           M  SWR++KLGFL KLKK+ LPGVD GSEI IPSH++CPISLDLMKDPVILSTGITYDRE
Sbjct: 1   MIFSWRKNKLGFLTKLKKQ-LPGVDCGSEIAIPSHYMCPISLDLMKDPVILSTGITYDRE 60

Query: 61  SIEKWIEGGKFSCPVTKQDLMSFDLIPNHTLRRMIQDWCVANHSYGIERIPTPRIPVTPY 120
           SIEKWI+GG FSCPVTKQ+L  FDLIPNH LRR+IQDWCVAN SYGIERIPTPRIPV+PY
Sbjct: 61  SIEKWIDGGNFSCPVTKQELTVFDLIPNHALRRLIQDWCVANRSYGIERIPTPRIPVSPY 120

Query: 121 EVNDVCSRIAVATQRGDSKRCSELVGKIRNWARESERNRRCVVNGGTGIVLAASFEHFSG 180
           EV ++CSRI++ATQR D KRCSEL+GKIRNWA+ESERNRRC+VNGGTG VLAASFEHF+G
Sbjct: 121 EVKEICSRISIATQRSDLKRCSELMGKIRNWAKESERNRRCLVNGGTGDVLAASFEHFAG 180

Query: 181 VSIEKHVGLLEEILLVLVCVFPISIEGLSKLGSADSLKCLVSFLMEKNLSPKQNAVFVLK 240
           VSIEKHVGLLEEILLVL C+FP++IEG+SKLGSA SLKCLVSFL+ K+LSPKQ+AVFVLK
Sbjct: 181 VSIEKHVGLLEEILLVLTCIFPVAIEGISKLGSAGSLKCLVSFLIGKDLSPKQSAVFVLK 240

Query: 241 ELLLVDQRYVDSLAAIEGVSEALVSIIREPLCPSATKSSLTAIFYMILPSEISEKMTLKF 300
           ELL  D+RYV+SLAAIEGVSEALVSIIR+PLCPS TKSSLT IFYMILPS+I EKM LKF
Sbjct: 241 ELLAADRRYVNSLAAIEGVSEALVSIIRDPLCPSNTKSSLTVIFYMILPSDIGEKMALKF 300

Query: 301 MELGLVSQLLDFLVDAEKSLCEKALGVLDGICDFKQGREKLYQNALTIPLLVKKILRVSE 360
           +ELGLVSQLL+FLVDAEKSLCEKALG+LDGICD+KQGREKLY NALTIPLLVKKILRVSE
Sbjct: 301 VELGLVSQLLEFLVDAEKSLCEKALGILDGICDYKQGREKLYNNALTIPLLVKKILRVSE 360

Query: 361 VASEYCLSILLKLCKSGEKGEDEARVEAAQLGAFQKIIVLLQVGCGGDMKNKVTEMLKLL 420
           +A+EY LSILLKLCKSGEKGEDE RVEAAQLGAFQKI+VLLQVGCGGDMK+KVTEMLKLL
Sbjct: 361 LATEYSLSILLKLCKSGEKGEDEVRVEAAQLGAFQKILVLLQVGCGGDMKDKVTEMLKLL 420

Query: 421 NLYKDRLDCIDSSMHFKFLKKSF 444
           NLYKDRLDCIDSSMHFK+LKKSF
Sbjct: 421 NLYKDRLDCIDSSMHFKYLKKSF 442

BLAST of Carg07457 vs. TAIR 10
Match: AT5G37490.1 (ARM repeat superfamily protein )

HSP 1 Score: 395.2 bits (1014), Expect = 6.9e-110
Identity = 210/423 (49.65%), Postives = 290/423 (68.56%), Query Frame = 0

Query: 22  PGVDSGSEIVIPSHFLCPISLDLMKDPVILSTGITYDRESIEKWIEGGKFSCPVTKQDLM 81
           P  +  SEI IP  F CPIS+DLMKDPVI+STGITYDR SIE WI  G  +CPVT   L 
Sbjct: 21  PESEPESEITIPPEFQCPISIDLMKDPVIISTGITYDRVSIETWINSGNKTCPVTNTVLT 80

Query: 82  SFDLIPNHTLRRMIQDWCVANHSYGIERIPTPRIPVTPYEVNDVCSRIAVATQRGDSKRC 141
           +FD IPNHT+R+MIQ WCV   S  I+RIPTPR+P+ P EV ++  +++ AT+RGD ++C
Sbjct: 81  TFDQIPNHTIRKMIQGWCVEKGSPLIQRIPTPRVPLMPCEVYEISRKLSSATRRGDYEKC 140

Query: 142 SELVGKIRNWARESERNRRCVVNGGTGIVLAASFEHFSGVSIEKHVGLLEEILLVLVCVF 201
             ++ KI+    ESE+NR+CV     G VL   F+ FSG   EK   +L EIL +L  +F
Sbjct: 141 GVIIEKIKKLGDESEKNRKCVNENSVGWVLCDCFDKFSG--DEKLTFMLNEILSLLTWMF 200

Query: 202 PISIEGLSKLGSADSLKCLVSFLMEKNLSPKQNAVFVLKELLLVDQRYVDSLAAIEGVSE 261
           PI +EG+SKL SA S +C+   L   + S +QNA F++KE+L +D+  V S A   GV+E
Sbjct: 201 PIGLEGISKLASATSFRCVAGLLKSTDDSVRQNAAFIMKEILSLDETRVHSFAVENGVAE 260

Query: 262 ALVSIIREPLCPSATKSSLTAIFYMILPSEISEKMTLKFMELGLVSQLLDFLVDAEKSLC 321
           ALV +IR+ +  S+TKSSL AI+ M+L      ++  +F+E+GLVS  ++ +VDAE S+C
Sbjct: 261 ALVKLIRDSVSSSSTKSSLIAIYQMVLQ---KPEIASEFLEIGLVSITVEMIVDAENSVC 320

Query: 322 EKALGVLDGICDFKQGREKLYQNALTIPLLVKKILRVSEVASEYCLSILLKLCKSGEKGE 381
           EKAL VLD IC+ + GRE++ +NAL +PLLVKKI +VSE+A+   +S++LKL K+G    
Sbjct: 321 EKALAVLDAICETEHGREEVRKNALVMPLLVKKIAKVSELATRSSMSMILKLWKTGNTVA 380

Query: 382 DEARVEAAQLGAFQKIIVLLQVGCGGDMKNKVTEMLKLLNL-YKDRLDCIDSSMHFKFLK 441
            E   +A +LGAFQK++++LQVG G + K K TE+LK++N   K   DC+DS   FK++K
Sbjct: 381 VE---DAVRLGAFQKVLLVLQVGYGEETKEKATELLKMMNTQMKLMSDCVDSLKEFKYIK 435

Query: 442 KSF 444
           K F
Sbjct: 441 KPF 435

BLAST of Carg07457 vs. TAIR 10
Match: AT1G66160.1 (CYS, MET, PRO, and GLY protein 1 )

HSP 1 Score: 359.8 bits (922), Expect = 3.2e-99
Identity = 207/434 (47.70%), Postives = 285/434 (65.67%), Query Frame = 0

Query: 17  KKEHLPGVDSGS--EIVIPSHFLCPISLDLMKDPVILSTGITYDRESIEKWIEGGKFSCP 76
           KKE +P        EI IPS F CPIS +LMKDPVI+++GITYDRE+IEKW E G  +CP
Sbjct: 16  KKEIIPVTSCSEEVEITIPSQFQCPISYELMKDPVIIASGITYDRENIEKWFESGYQTCP 75

Query: 77  VTKQDLMSFDLIPNHTLRRMIQDWCVANHSYGIERIPTPRIPVTPYEVNDVCSRIAVATQ 136
           VT   L S + IPNHT+RRMIQ WC ++   GIERIPTPR+PVT ++V+++C R++ AT+
Sbjct: 76  VTNTVLTSLEQIPNHTIRRMIQGWCGSSLGGGIERIPTPRVPVTSHQVSEICERLSAATR 135

Query: 137 RGDSKRCSELVGKIRNWARESERNRRCVVNGGTGIVLAASFEHFSGVSIEKHVGLLEEIL 196
           RGD   C E+V K+    +ESERNR+CV   G G+VL   F+ FS  +      LLEE +
Sbjct: 136 RGDYAACMEMVTKMTRLGKESERNRKCVKENGAGLVLCVCFDAFSENANASL--LLEETV 195

Query: 197 LVLVCVFPISIEGLSKLGSADSLKCLVSFLMEKNLSPKQNAVFVLKELLLVDQRYVDSLA 256
            VL  + PI +EG SKL +  S   LV  L   +    QNA F++KELL ++  +V +L 
Sbjct: 196 SVLTWMLPIGLEGQSKLTTTSSFNRLVELLRNGD----QNAAFLIKELLELNVTHVHALT 255

Query: 257 AIEGVSEALV-SIIREPLCPSATKSSLTAIFYMILPSEISEKMTLKFMELGLVSQLLDFL 316
            I GV EA + SI R+  C     +SL +I +MIL    +++   +F+EL LV+  ++ L
Sbjct: 256 KINGVQEAFMKSINRDSTC----VNSLISIHHMIL---TNQETVSRFLELDLVNITVEML 315

Query: 317 VDAEKSLCEKALGVLDGICDFKQGREKLYQNALTIPLLVKKILRVSEVASEYCLSILLKL 376
           VD+E S+CEKAL VL+ IC+ K+GREK+ +N L IP+LVKKIL++SE   +  +S++ K+
Sbjct: 316 VDSENSVCEKALTVLNVICETKEGREKVRRNKLVIPILVKKILKISE--KKDLVSVMWKV 375

Query: 377 CKSGEKGEDEARVEAAQLGAFQKIIVLLQVGCGGDMKNKVTEMLKLLNLYKDRLDCID-- 436
           CKSG+  E E   EA +LGAF+K++V+LQVGCG   K KVTE+LK++N        +D  
Sbjct: 376 CKSGDGSEVE---EALRLGAFKKLVVMLQVGCGEGTKEKVTELLKMMNKVMKMNGFVDRS 431

Query: 437 --SSMHFKFLKKSF 444
             SS+ FK +KK F
Sbjct: 436 YSSSIEFKHVKKPF 431

BLAST of Carg07457 vs. TAIR 10
Match: AT1G66160.2 (CYS, MET, PRO, and GLY protein 1 )

HSP 1 Score: 323.6 bits (828), Expect = 2.6e-88
Identity = 195/434 (44.93%), Postives = 269/434 (61.98%), Query Frame = 0

Query: 17  KKEHLPGVDSGS--EIVIPSHFLCPISLDLMKDPVILSTGITYDRESIEKWIEGGKFSCP 76
           KKE +P        EI IPS F CPIS +LMKDP                W E G  +CP
Sbjct: 16  KKEIIPVTSCSEEVEITIPSQFQCPISYELMKDP----------------WFESGYQTCP 75

Query: 77  VTKQDLMSFDLIPNHTLRRMIQDWCVANHSYGIERIPTPRIPVTPYEVNDVCSRIAVATQ 136
           VT   L S + IPNHT+RRMIQ WC ++   GIERIPTPR+PVT ++V+++C R++ AT+
Sbjct: 76  VTNTVLTSLEQIPNHTIRRMIQGWCGSSLGGGIERIPTPRVPVTSHQVSEICERLSAATR 135

Query: 137 RGDSKRCSELVGKIRNWARESERNRRCVVNGGTGIVLAASFEHFSGVSIEKHVGLLEEIL 196
           RGD   C E+V K+    +ESERNR+CV   G G+VL   F+ FS  +      LLEE +
Sbjct: 136 RGDYAACMEMVTKMTRLGKESERNRKCVKENGAGLVLCVCFDAFSENANASL--LLEETV 195

Query: 197 LVLVCVFPISIEGLSKLGSADSLKCLVSFLMEKNLSPKQNAVFVLKELLLVDQRYVDSLA 256
            VL  + PI +EG SKL +  S   LV  L   +    QNA F++KELL ++  +V +L 
Sbjct: 196 SVLTWMLPIGLEGQSKLTTTSSFNRLVELLRNGD----QNAAFLIKELLELNVTHVHALT 255

Query: 257 AIEGVSEALV-SIIREPLCPSATKSSLTAIFYMILPSEISEKMTLKFMELGLVSQLLDFL 316
            I GV EA + SI R+  C     +SL +I +MIL    +++   +F+EL LV+  ++ L
Sbjct: 256 KINGVQEAFMKSINRDSTC----VNSLISIHHMIL---TNQETVSRFLELDLVNITVEML 315

Query: 317 VDAEKSLCEKALGVLDGICDFKQGREKLYQNALTIPLLVKKILRVSEVASEYCLSILLKL 376
           VD+E S+CEKAL VL+ IC+ K+GREK+ +N L IP+LVKKIL++SE   +  +S++ K+
Sbjct: 316 VDSENSVCEKALTVLNVICETKEGREKVRRNKLVIPILVKKILKISE--KKDLVSVMWKV 375

Query: 377 CKSGEKGEDEARVEAAQLGAFQKIIVLLQVGCGGDMKNKVTEMLKLLNLYKDRLDCID-- 436
           CKSG+  E E   EA +LGAF+K++V+LQVGCG   K KVTE+LK++N        +D  
Sbjct: 376 CKSGDGSEVE---EALRLGAFKKLVVMLQVGCGEGTKEKVTELLKMMNKVMKMNGFVDRS 415

Query: 437 --SSMHFKFLKKSF 444
             SS+ FK +KK F
Sbjct: 436 YSSSIEFKHVKKPF 415

BLAST of Carg07457 vs. TAIR 10
Match: AT3G02840.1 (ARM repeat superfamily protein )

HSP 1 Score: 268.9 bits (686), Expect = 7.5e-72
Identity = 154/344 (44.77%), Postives = 231/344 (67.15%), Query Frame = 0

Query: 107 IERIPTPRIPVTPYEVNDVCSRIAVATQRGDSKRCSELVGKIRNWARESERNRRCVVNGG 166
           IERI +PR+ +TP +  ++  R+  A  R +   C E+V KI+N  R  + N++C+V  G
Sbjct: 40  IERIQSPRVLLTPRDAVEISRRLQNAAAREEYAECLEIVSKIKNLGRGGDTNKKCLVQNG 99

Query: 167 TGIVLAASFEHFSGVSIEKHVGLLEEILLVLVCVFPIS-IEGLSKLGSADSLKCLVSFLM 226
           + + L++ FE F+    + H+ LLEEIL VL    P++  EG +K+GS  SL CLV FL 
Sbjct: 100 SVLALSSCFERFAAAR-DGHMRLLEEILFVLSSWLPLNRSEGFNKMGSTASLNCLVRFLN 159

Query: 227 EKNLSPKQNAVFVLKELLLVDQRYVDSLAAIEGVSEALVSIIREPLCPSATKSSLTAIFY 286
            K+   +QNA F ++E++ VD+RYV +L  +EG  E LV IIR+ +  S+TK+SL  I+ 
Sbjct: 160 GKDAKTRQNAAFCIREVIAVDKRYVYALTDVEGACEGLVKIIRDSVSTSSTKASLMVIYR 219

Query: 287 MILPSEISEKMTLKFMELGLVSQLLDFLV-DAEKSLCEKALGVLDGICDFKQGREKLYQN 346
            I     ++K+T KF++LGLV  + + +V +AEKS+CE++L VL+  CD +QG+E + +N
Sbjct: 220 AI---SSNDKITEKFVKLGLVELITEMMVNNAEKSVCERSLVVLNATCDNEQGKEDVLRN 279

Query: 347 ALTIPLLVKKILRVSEVASEYCLSILLKLCKSGEKGE--DEARVEAAQLGAFQKIIVLLQ 406
           AL +PLLVKKILRVS++A++  +SIL KL K+ + GE  D   VEA Q+GAF+K++VLLQ
Sbjct: 280 ALIVPLLVKKILRVSDLATQCSVSILWKLWKNKKDGECDDRLLVEALQVGAFEKLLVLLQ 339

Query: 407 VGCGGDMKNKVTEMLKLLNLYKDRLD---CIDSSMHFKFLKKSF 444
           VGC    K K +E+L+ LN  ++ ++   C+DSSMH K +KKSF
Sbjct: 340 VGCEDKTKEKASELLRNLNRCRNEIEKTNCVDSSMHLKNVKKSF 379

BLAST of Carg07457 vs. TAIR 10
Match: AT1G49780.1 (plant U-box 26 )

HSP 1 Score: 211.8 bits (538), Expect = 1.1e-54
Identity = 141/398 (35.43%), Postives = 210/398 (52.76%), Query Frame = 0

Query: 30  IVIPSHFLCPISLDLMKDPVILSTGITYDRESIEKWIEGGKFSCPVTKQDLMSFDLIPNH 89
           I IP HF CPISLDLM DPV +STG TYDR SI+ WI  G  +CPVT+  L  F LIPNH
Sbjct: 12  IQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGNTTCPVTRVALSDFTLIPNH 71

Query: 90  TLRRMIQDWCVANHSYGIERIPTPRIPVTPYEVNDVCSRIAVATQRGDSKRC-SELVGKI 149
           TLRR+IQ+WCVAN S G+ERIPTP+ P  P  V  + S+ +  T    S R  +  + ++
Sbjct: 72  TLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRAAAIRRL 131

Query: 150 RNWARESERNRRCVV-NGGTGIVLAASFEHFSGVSIEKHVGLLEEILLVLVCVFPISIEG 209
           R  AR+SE+NR  +  +    I++   F      S+     L+ E L +LV +     E 
Sbjct: 132 RGLARDSEKNRVLIAGHNAREILVRILFADIETTSLSSE--LVSESLALLVLLHMTETEC 191

Query: 210 LSKLGSADSLKCLVSFLMEKNLSPKQNAVFVLKELLLVDQRYVDSLAAIEG---VSEALV 269
            +       +  +   L + ++  + NA   L E++L   + +D    I G   + E ++
Sbjct: 192 EAVASDPSRVGFMTRLLFDSSIEIRVNAA-ALIEMVLTGAKSMDLKLIISGSDSIFEGVL 251

Query: 270 SIIREPLCP-SATKSSLTAIFYMILPSEISEKMTLKFMELGLVSQLLDFL-VDAEKSLCE 329
            +++ P+    A K  + AIF + L  +         +  G    L+D L  D ++   E
Sbjct: 252 DLLKNPISSRRALKIGIKAIFALCLVKQTRHLA----ISAGAPGILIDRLAADFDRCDTE 311

Query: 330 KALGVLDGICDFKQGREKLYQNALTIPLLVKKILRVSEVASEYCLSILLKLCKSGEKGED 389
           + L  ++ +C   +G     ++ALT+PL+VK ILRVS+ A+EY    LL LC +    E+
Sbjct: 312 RGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGALLALCTA----EE 371

Query: 390 EARVEAAQLGAFQKIIVLLQVGCGGDMKNKVTEMLKLL 421
             R EAA  G   ++++L+Q  C    K K   +LKLL
Sbjct: 372 RCRDEAAAAGLVTQLLLLVQSDCTERAKRKAQMLLKLL 398

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6607019.11.6e-249100.00U-box domain-containing protein 21, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_023523202.17.4e-24798.87U-box domain-containing protein 21-like [Cucurbita pepo subsp. pepo][more]
XP_022949556.11.6e-24698.65U-box domain-containing protein 21-like [Cucurbita moschata][more]
XP_022997974.13.7e-24698.42U-box domain-containing protein 21-like [Cucurbita maxima][more]
XP_038899398.16.9e-22186.68U-box domain-containing protein 21 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q5PNY69.7e-10949.65U-box domain-containing protein 21 OS=Arabidopsis thaliana OX=3702 GN=PUB21 PE=2... [more]
Q9C8D14.5e-9847.70U-box domain-containing protein 20 OS=Arabidopsis thaliana OX=3702 GN=PUB20 PE=1... [more]
Q9FXA41.5e-5335.43U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2... [more]
Q84TG33.8e-5236.45E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 ... [more]
Q9LT796.0e-5035.82U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2... [more]
Match NameE-valueIdentityDescription
A0A6J1GCB68.0e-24798.65RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111452870... [more]
A0A6J1KD231.8e-24698.42RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111492767 P... [more]
A0A0A0LAV47.5e-22186.91RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G308190 PE... [more]
A0A5D3BS092.9e-21785.55RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3C4622.9e-21785.55RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103496814 PE=4 ... [more]
Match NameE-valueIdentityDescription
AT5G37490.16.9e-11049.65ARM repeat superfamily protein [more]
AT1G66160.13.2e-9947.70CYS, MET, PRO, and GLY protein 1 [more]
AT1G66160.22.6e-8844.93CYS, MET, PRO, and GLY protein 1 [more]
AT3G02840.17.5e-7244.77ARM repeat superfamily protein [more]
AT1G49780.11.1e-5435.43plant U-box 26 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003613U box domainSMARTSM00504Ubox_2coord: 35..98
e-value: 1.9E-31
score: 120.5
IPR003613U box domainPFAMPF04564U-boxcoord: 32..102
e-value: 2.5E-21
score: 75.6
IPR003613U box domainPROSITEPS51698U_BOXcoord: 31..105
score: 41.143116
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 17..109
e-value: 1.2E-29
score: 104.2
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 127..419
e-value: 1.8E-11
score: 45.7
NoneNo IPR availablePANTHERPTHR22849:SF61U-BOX DOMAIN-CONTAINING PROTEIN 21coord: 26..443
NoneNo IPR availablePANTHERPTHR22849WDSAM1 PROTEINcoord: 26..443
NoneNo IPR availableCDDcd16664RING-Ubox_PUBcoord: 36..78
e-value: 4.47712E-25
score: 94.993
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 29..108
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 206..420

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg07457-RACarg07457-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0004842 ubiquitin-protein transferase activity