Carg07431 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg07431
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionNicastrin
LocationCarg_Chr01: 1752954 .. 1764745 (+)
RNA-Seq ExpressionCarg07431
SyntenyCarg07431
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TATTTATTTTGAGGGCTTTCCTTTTACCCGTTCTTTCCACTCCCGATGATCAAATTTCAGTTCAATTTCCCAACTCTACGTGGGCTCTCGAGTCCATTGCAGGGACACATTCATCTTCGTCTTGCGCAGAGCTGAGGTCCCCAAGAACGCTCCAATAGACTGCGATTCGAATCAAAGAATAGCCAAATCAGCATCCATGTCTTTCCATATTCTTCACCTCCTCCTGTTTTTAACTTCTGTTCGTCTCTGTTTATCAGGTGCGTTAAAAGTGAAGGGCTATCAAATTTTTGTTTCAATGGCTTCTTATTGCTCGTTGACCATGAATGCCAATCAGTCTTGATGTCTTAAAAGTAATGTAACTAATGTGTATGGGTTACTTCCTTACATTTCGTAATCTGGGTAGTCATTAGCAGCTAAAGGGTGCGTTATTTGAAGTAATTAGATGCCATTTTGCTGCTTGATGTCTAAATTTCACCTCTGAAGGTTTTGTTTGCTCGGATGTTAGTTTTCTGCTTCTTTTCAAGTTCTTATTGCTTATACGAAATTATTTTTATACTAATAAGATTATATTCTATCCCCGCGAAGCTAATTATCTGGTTCATACGACAATATCACACATGGATGAAGGATAATTATGGGTTTAAGTAAATTTTATCCTTTCTATTATAACTTTATAAATTTTGGAGTGAGCATTTTGATGTTGACTATAGAGCATGAATCCTGACCTGTTATCATTTTCTGTAATGCATTCAACAGATGAACACTCAATGGAATCGGTTCCTGATCTTCAAAATTCAATGTACCTTGTAGTTGATGGTTATCCGTGCATTCGATTACTCAATCTCTCTGGAGAAATCGGTTGTTCAAGTAGGTTATTTTGCACTATACGTAGAAAATCTATTACCTTTGGAAATGCCAACGTAATCCTTAATGGTATGGAGGCGTCATTCATGTATTTTTACTATGTGTACCGCAGATCCTGGGCGAGAAAAGGTTGTAGTACCCATGATTAACTTCAAAGATGCTGATGAGATATTGCAACCGTCTGCAATTTTAGTTTCAATGGATGACATCTCGAGTTTCTTTGCTAGGTAGGTTTTAGATTTTTATATATACTCCCTAGCTTCATACATATTCTCGTTTCATTATAAGGATTATTTTCTAAACTTGGGTCTACAACATGTTCCAAGCCTCCCAATGAAAAATTAGAAATAAGTGAAGAAGCATTCTCATCTACAAAAGAAAATAAAAAAGAAAAGAAAAAAAAAAAGAGAGAGATTTTGAGTGGAAAAATAAATCGGAAAATCAAGGTCTCCTTTGGGCCTCTTGTACTATAATCTATCACCACTAGTGATTGAATTCATATAAGCAGTCCTCAATTGCTATTTTCATTGCAAATTATATGAACCGTTTCTCCAGTGGGAAGATATAAATGATCTCTTCCTCCATTTTCTTCTCTGCTTATCAGAGTTGGAAGAAAACTATCTACTCAGGTAGATTTTTTTTTTTTGGAAACCTATTTCAGATTACAGGATGATTCCAATTTTGCAAGTAATGTTGGTGGTGTTTTAATCAAACCGGGGACTGAAATACAGAAAAGAACGAAAGGTAGAGTTCATTTATCTATTCAGGTTCTTTTCCCCCCTTATTTCTTTTCTTTCCTTTTCTTTTTTTTGTAGTTGTGTGTAATTCAGACATGCACGTCAATAACTTACGTGGTGATTTATTTTCCTTTGCAGGGTTTTCTCCTGCTCAAAAGTTTCCACAAGCTAAATTTGCTCCTTACCAAAAAATTGACTATGAATGGAACCCAATTGTATGTTTGAATTTTCTTGCTGAGTTTTTTATTTTTTTTAGGAGTGGTCAACCTTTAGAAGCTTTTTACTTCTGAACTCTTATTTTGTTCTCACTTATAAAGCTAAGCACCAAACTATTGATTGTTGGTTGATATCATATCCGCCATATGATTATTATGAGTTAATCTCAGCCTTCCATATATGAGTAGAAGCTTATAAGTATTTTGTTAGAAGTATTCAGCTTTTAGCTCCTTGGTGGAGCCTTTTTCACAAGGATTCCTCGTGTAACTATAGCCTCTCGATGATTATTAGAATTGGAAGACCTCTTTGTAATAGTTTTCACTTTTGGGTTGGGGATTTCTCTATTTTTGTGTGGCTTTTTTATACAAGATATATCTTCTATGAAAAGATAAATAATAATAATAATTAGAAGCTGGTAAGAGGTTTGTCGGCCCATGAAGTCATTAGGGCTGATCTGTGTTTAAATTTGGAGCTACTACTGACTCAAGGGTTTATCTTAACTGAGTCTGAATTCTGATTTTTCTTGAAAATCAGCCTCCATCGCATCACTTTGAAAAGAGGCTGAATTCTGTAATTACTATCTTGGTCTCATTTTTTAAGTTACAATTTCCTCCTGTAGTTCATCGGGACTAGTTTTGTGAGTTTATCTTTTTTACACCCTATACATTCTTTCATTTTTCTCAATGAGTGGCTTAGTTTCTTATGAAAAAAAAAAAAAAAACTTTGTTTTATCACAGGCTTTCATTTCTTCTTTGGCTTGACAATCTGTAGCATCTTACAATTTGCACTTGCAGGGATCTGGAGTTATGTGGAATCAATATAACTTTCCTGTTTTCTTAATATCTGAGAGCAGCATTTCCCCTGTACAGGAGGTAATTCTGATTCTTAATCACTCAAAACGTTCAATTGCCTTGAGATATTTCAATTACCACAAGGCTGTTTGGTTCAGTTGTTATATGCTTGTCTCTGGAAATCAATTTTTTATTTTGATTAATCTGATGGTTGCCATCAGACATTCCTTAGTCTTTCACATTGGTAAGATCATAATGTACTTTTACAGTTTTTTTTTTAAATGGTTAGCAAAACTGAAATAAAAACAACCCAAAATAAAATGTAGAACCGTTTATGAATAAGTGCATTTGTCATAGGGTTGAAACTTAATGATATTTTGCAATTATGTTTACATTATTTGCTAGCGGTGGGCTTGGGCTGCTACAATTGAAGAGATATTTTGTTCTTAAAAGATAATTATGCCAATACTTTGACCAGAAGAATTTATCTGTTGCACAATCGTACCACTTAATGCAGGCTGCTTCAAAAAATGTGAAGGACAAGAAAGTTTACACGTCTAATGTTGCTGAATTTGATCTGGTGATGCAGGTACTTAGCTTCTGGTGTTACAAATGATTACATTTCAGTGATTTCACATCTTAAATGGAATCACACTTTGCAAAGTTCGTTTAATTCATTTTCTTTTTGTCTAGACAACCAAGGCTGGAACTCGTAATTCAATGTCTTGTTTGAAAGAAGAAACATGCCTTCCGTTAGGTGGATACAGGTTAAGTTTTATTTTATTTTTAGTTTTATTTTTGTCTCCACATAGTTTTAGAATTTTATTCTGTTTAATTTGTTAAAAGAGATCAGTATCTTTACAGTGTCTGGTCGTCGCTTCCTCCAATCAATATTTCTTCAGATAAGTCTAAGCCCATCATTCTCACAGTAGCTTCAATGGACTCTGCGTCTTTCTTCCGTGATAAAAGCATTGGTGCCGACTCCCCCATCTCTGTATGATTTATGTTGAGCAAGTATATTAGTCATAAACCTTACCTGTTTATTCTGCTACCTGTTAAAGTCTTTCTGGTACAATTTAGGGCCTGATTGCATTGCTGGCTGCGGTTGATGCACTTTCCCATGTGGATGGACTGGATGATCTTCATAAACAGGTTCTTATAGAAGCTATTTTCTTTGTATCCTATTGTACTGTATTTTTAATAGTAGCTATTAACTCAAATGCAAATATTCTATAATCTTTTCATCTACATAATTGCATTTTAGATATAACTGTTATGTCTGACTATGTAGAATTTGAGATATAATTGCTATTTCTTGTTTTTATTGCCTTCTATTGATTGAGTTATTGGATTGTCTTATCTTATTTAATCTTAGTTACAACCTCTCCAAATTAATTTTGAAATGGAGAGAAAAAAAAAAACCCATTTTTCATTCCTACAAAGGACATCAGAAACACAAATTGGTGGAGAAGAAATTACCGTGGTAATAGGTTTCTCGCCAACACAAGGAGTTCTAGCCACGGAAGGCCTACTTGAAAAATTCAGTAACGTTGGTGTTCAAAGACTGAATAGACCTACGTATGATCATTATCCCTTTCTACTTTCCATGGGTTGCTGTAAATGGGGCCCTTCGCCCTTCCGGTTTGAGAATATGTGGCTCCACTTTGTTTGCTATGGTGTAGAGTATTGGGAAGAATACGCCCCTTCGTGGATGATCGGAGACGGTTTCATCAACAAACTTAAAAAACTAAAAGAAGTCTTAAAGAATTGGAACAAGGAGGTTTTCGGTTGTATCTCCACAAAAAGAAACCAATTACTTACTGAAATTGTCCTCCTTGATCAAATGGAGGAAACCGACTCTATTACACTAGTTCAACACACATATAAAAAGCTCATGAAAGTAGAACTTATTTCGTTGGCAGCAAAGGAAGAGCAATCTTAGAGAGAAAATGTAAAAAACGATGGTCAAAGGAGGGTGACATAAACCTCTAATTTTTCATTGTATCATGCAAAGAAACAAAAGAGTACCATCGTGGAAATTCTATCCCCATAAGGCAGAAGTTTAGTAAATGAAGATGAAATTGTCTCATAATTTGTGTCCTTTTACACGTCCTTATATACAAAGGACAATGCCCTCTTTGAATTTTCCCACGATCTTGACTAGGATCCTATTGATCAACCGCAAGCTGCCTCCCTCAAGGTTATTTTCACCAAACAAGAGGTGGGGAAGGTGATTCAGCATTCGGGATTCGACAAAACTCCAAGACTGGATAGTTTTACCTCATAATTTTTATAAACCGTTGGAACTTTATGAGAGTTGATTTCATGAGAGTATTCCAATATTTTTTTCGAATCAGAGTTATTAATGAAAACTTAAATGAAACATACATTTGTCTGATCTCAAAGAAACTGGATGCCTGCACTGTTGCAGACTTTCGTCCCATAAGCCTTACGACCGGACTATATAAAATCATAGGAAGGATATTATCAGAACATCTTAAAAAGGTTCTCCCTCTCACAATTACCGGGCAACAAACAACTTTTGTAGAGGGTGACGATGCATCTCTCATGGCAAACGAACTCATAGACGAATGAAAAAAAAAAAAAAAAAAGGTCTAGTCATCGAACTTGACACGGAAAAGGCCTTCGACAAGGTCGATTGAACTTTCCTTGAAAACATTCTTGTGACAAAAGGATTTGGCTCAAAATGGTGAAGGTGGATAAAAGGATGCATCTCGTCCACAAACTTCATCATCATCAATGGAAGATCAAGAGGGAAGATTATGCCACACGGGGTCTTCCACGAGGGGATCCACTATCCATTTACTTTTCATCATGGTAATGGACTGCCTCAGTCGCATACTGACAAAAGCAGAATACGAAGGGCAGATAAAAGGTTTTCAGATTGGTAACGAGGGCTTAAGCATCAACCACCTTCAGTTCGCGGACGATACAATCCTCTTTTCTGACTTGGCAAATATCTCTTCCATAAAAGACGTGATCGAACAGTAAAAACCTTCAAAGGATTTTCTGGACAAAATATCAATCTCCAAAAAACAGAGATAATGGGCATCAATATTAACACAGATTATTAAAGAATTTGCTAGCATATATGATTGTAAAAATGGAGAATGACCGAATATTTACCTAGGATTACCTCTAAAAGGAAACCACAAATATTTTTCCTCTTGAAAGACTATTATTGAAAAAGTAGAAAGAAGGTTATCAACATGGATATCATGTTATACTTCAAAAGGTGGAAGACTCACCATAATACAAGCTACATTATCCAACCTCCCCACTTACTACATGTCTCTATTTGAAATGCCATAAAAAGTGGTTTCAGATATAGAAAGATTATTGAGAAACTACTTGTAGAAAGATGGCCCACACCTTTTTCGATGGAAGATTATAAACCTCCCAACAGTAAAGGGAGACCTTAGTCTCTGAAAGATTATTGAGAAACTACTTGTAGAAAGATGGCCCACGCCTTGTTCGTTGGAAGATTATAAACCTCCCAACAGTAAAGGGAGGCCTTAGTCTTTTCTCGATAAAGAAGAAGAACAAATCTCTCCTCGCCAAATGGATATGAAGATATCATCACGAAGAAAAAGTGTTGTGGAGAAATCTTATAAAGGCTAACTATACTCCAACATCAAACAAAAATCAATCTCTTCCCTCTTCTGCAAAAGAGCCTTGGAAGTACATAAAGAAACATCAAAACCTCATCACCAACCGAACTTTTCATAGGGTAGGTGATGGAGGAAGCACATCATTTGGATCTACCCAGGAATTGAAAACACCACGTCCATCAAGGATTTTGTAGAGAAGTGTGGTGTTCTCAATTCATGGGTCGGTCCAGAATGATGTGCTCCTTCCATCACCCACCATATGGCGAATTGGGTTGGTGATGAGGTTTTGATGTTTCTTTATATACTTTCAAGGCTCTTTTTGTAGATGATGAAGGAGATTGATTTAGTTTGATGTAGGAGTATTTAGCCTTTATAAGATTTCTCCTCAATACCTTTTCTTCGTGATGATATCTTCATATCTATTTGGCGAAGAGAGCTTTGTTCTTCTTTATCAAGAAAAGATCAAGGCCTCCCTTTTCTGTTGGGAGGTTTATAATACTCCATCAAACAAGTTGTGGGCCATCTTTCTACAAGTAGTTTTTGAATAATCTTTCTACAAGTAGTTTTTGAATAATCTTTCTATATCTGAAACCACTTTTTGTGGCATTTCAAATAGAGACATGTAGTAAGTGGGGAGGTGGATAATGTGACTTGTATTAGGGCGAGTCTTTCGCCTTTTGCAGTATAACAGGATGACCATGTTGATAGCCTTCTTTCTATTTTTTCAATAATAGTCTTCCAGAAGGAAAAAAATTGTGGTTTCCTTTTAGAGGTAATCCTAGGTACATATTCGGTCATTCTCCCTTTTTACAACCATATATGCTAGCACTTTCTTCAATAATCTCTGTGCTAATAGTCTTCGAAGGTAGTAAATTTGTTTCAATGAAAGGTTTTTGGTTTTAGAATTATAGAGAATAAAGCCCGCTGGTAGTAGTTATTGTTGTTGGTCCTTTCTGTTTTATTTTACACACTTAGTTTAGTGGTTCTATCCCTAAGATGCCCGAATTTATATGCTTATGTATCATTTTTTATATATATGTTTTAGCATTTATGAAATGTATTGGAGGTTTGATGATGATGCGTGGGATATAATCATCCCAAGCTCATATGCAGATTGTCTATAATTTGTATCCATTCTTTGGCTTCATTCTGATTGAATACATAGATCATGGTGCTTTAAGTCTTGTTTGCATCCTTTGCCTTTTCTTATTATTCAGTTGCTAATAGTTATTCGTGAATATCCAGCTTGTTTTTGTTGTCTTCACTGGAGAGTCTTGGGGCTACCTTGGTAGTAGGAGATTTTTGCTTGAACTTGATTTACAGTCCGATTCTGTCAGTGGCCTTAACAATACATTGATCGATACGGTATATTTCTGAACTTGTAAATTAGTGCTCTGAATTCTAGTTTTTCTTGTACTCGTTTCAGTGTAGAATTATAGTCACTCTCTAAGAGAAAAATATATGTTTTCTACTTTTCTTGTTTGAGTGGCTTCCTAGTGCGTTACCATTGGAGAAATACATAATGGATGTAGGGAATCATCAGAAAATACTAAAACAATTGGTTTAATATGTGTACAGCTCTTTATGTTTTTGGTGGGTGGATGTTTATCTTTGAAGCTTTTCTCTTTATTTTCAAAATTTTATTTGCTTAATTTGTTCTTCCCAATCGAGAAGATAAATATTTTCTTAGTCTAAACCAGTGCAAGAATAGGAAAATTGAAAATGCGTCTACTTATATTGAACAAAGAAAATTCAAGAAGTGCATGGTTTTCAAGCATCTGAAACCCTACCTATTATTGATGGTGGCTTGTTGCCGGGATAATTTATGTAAATTGGCAATTGCATGTGGGTGAGTGAAATACATTGACAATAAATTTTCCCCTCATATGAGGTAAGGAAAGTGATGCTACACAGGCTTCTGGTTGAGGAAAATAAGGACTTCATGCAGTCATATTTGAAGTGATAAAGCATGGCATGCCCTTTGAATGACATGATACAGATTTGACATGATGTATGAAAAGTGATATATATTTTAGCTAGAGCTGCATTTATCTGGTTGTTTGTTGTTTTCCAGTGCGTGTGTGATCTTTCTTTTTGTTTTTGATACTTTAGATCCATTTTATTTAAGTTAATGATAAAAAGTTGAACTTATTGTGCTTTTACCTTTTCAATTTAGGTTTTCGAAATTGGCTCTGTTGGAAAGAAATCCAATGATGGATTTGGAAACTTCTTCGCTCACATGACAGAGGTAATGGTTTTTTTTCCTCTAAACTGTTTTATTATTATTATTGTAAGGTTTCTCTATACTGTGAGACCTCCTATCATGCATACTTCCCAAAGTGTAAAAAGGGAATAGAATGATTTTTTTGTTAGGGTGGGAACCATTAGGATTGTGCGTTTGTGTACGGTCAGTGTGGGTAAGGTTTTTGTAGGGCTTGTGTTGCATGTGGCTGTTATGTTTAATTGGACATTTTATGTCTTCGGAGAGATTTCCAGCTCTCTCAATTAACTGGACTGTTTGAGTATTGTATTGCTTGTTTTCCCTAGTTGATTGCAATATCTTTGGCTGAAATAACCCATTGGTCATTGAAGAAATACTTATTGAACGGTGTTCGCTGTGTATTTAAGGTTATTTTGTGTTAGATACCAAACATACACATCTGAAATGTTGCTGCAACATTTAAAATCTTGTACTGTATGTTAGGCCTTTTGAGGGGATAATTGAACGTGGAGAATTGCAGAAATATTTTGAATTCCTTAAAATTACTGTCGTAATTGTTTTCTAATGTTTGAGTGAGAAGTTTGTAAGGTTAATATAACATCTAAAATATTGAGAAAATAATTCGAAAAACAAAGCAGCAGCTTTTATGGAATTTGAGAATCTTTATGACATTCTTAAACCCTCGAGAGTCTGAGACCATTATTTTTCTTCATAGATACTTGATCTATTTTGATATACTAGTTCGATATGATGCCCTAGTAATGTCATTAGCTATTCTGTTAGTATTTTATTATGCACGGAATAACAGTCATGTTCGATGAAAGATTTTATTTCAACCTATTCTTTTAGCAAGATATTGCTCCTGTAGGTTTCATCTTCCAAGAATGAGACATGGAACGCCTTGAAGCTTGCTCGAGAGTCGCTTCCATTTGAGAACATAAAAGTCTCACCAGCTAGTACTACAAATCCAGGGATACCACCATCTTCGTTGATGGCATTTCTAGCCAAGGTATTTTTCAACAACATACTTGATGCTACTATTTTCTTGCTCTCTATTGTTCAAACCTGCTGATTTTTTTATTGAAAAAAAAAAATTAAAATAAGAAACGAATGGCACACAATTCAAGGTTTGAGACTAGAGTTTTGCGAAACAATTTCATTGATGTATAAAATTTATAAAAAGGATAATAATCCATGATGATTACGTAACACTTCTCCAATTGGTTGAAAGGGAAGCATAGCTATATGAAGTAAAGATATTAGACCGTGTACACCAAGAGAGATAAGCTGACAAGTTTATTTTGGTCCGTACAGAACTCGCAGGTCTCTGGGGTGGTATTAGAAGACTTTGATACTAGCTTTACCAATCAATTTTACCAGAGTCACCTCGACGATTTACGTAAGTTGCATTCTTTTCTTTCCTTCCTTTCCCATTTTGTCGCTCATTGGCGATTGTTGTATAGCTGGTCCTATTTTTTCTTACTATTCATATTATTTTTCAATCCAGATAATATAAACTCATCAGCTATTGAAGCAGCTGCTTTACTTGTTGCCCGAACTCTTTACATTCTTGCAACCAACAAAAAGGAATTGAGTAGTTCTGCCCTGAATGCTATCAAATTGAACACCTCGTTGGTTGAAGAGCTTATAGGATGTCTCCTGAACTGTGACCCTGGCCTCTCTTGTGAGTTGGTGAAGAGATATATTTCTCCGACCAATGTCTGTCCAAACCATTATGTTGGTGTTATCCTCGATGAACCATCCTCTACTCCTTATCCTGGTTATGTTCACGATGTTTCAAGATTTGCTTGGAACTTTTTAGCTGACAGAACATCCATCCGTAAAGAGAATACTAGCTCTGTCTGTTCACAGAACTGTGATGACAAAAGTGAGGTGTGCATTGGAGCGGAGACTGGAAAGGGAACTTGTGTTGTATCAACTACCAGGTACCATCATATTTATAGCTAGACACAACTGATTTAGAATGGTGCATTGGTGGACACATGGCATACTTGGTCATTGCATCAAAACTAAAACAAAATAAAACAAAAAAAAAAACTTAAATTAAGATAACTTAGAGAATATTTAAAACTGCAGGAGGCCTTGAGGAAGGATTGTCCTAGAGAGTATGTTGAAGCCTGTAATGTAGGAAAAAGTTATTTATTGAAGCTGACACAATGTGGCTGTCTCTTATAACTCGTGGATTTAAAAATCTTACTAATTTGAGATAGTTTTGCAGTCTCATGCATTCCATGGTTCAAAACTGCAAGCTACCTTGAATAATTTTTCGTAGTATAGAGGTTGTAAGTGGGTTGATTCGACTTGACTAAATTGACGTATGACTACGAATGGGAAGTCTGGAACGTCTACATTACAGATTGTTTGATTAGTTGTCCATGCTCTAATTGTTTTATTATTACTTTTTTTTGGGGGGGGGGGAGTAAAAAACAGGTTCAATATGTAACACTACTATGCAAGTTTGATAACAATATTTAAAAGTTTGATTAGTTGTACAACTGCCTTTCTTTGTTAAATATCTGTAGTTGTTTGCAACAGGTACGTTCCAGCATACTCAACACGATTGACGTTCGATTCTGGATCTTGGAATGTGCTTCCTCCGAATGCATCGGACCCAATGGGCGCTGTCGATCCTGTTTGGACAGAGAGCAACTGGAACACCATAGGACTCCGAATGTATACCGTCCAAGCTACTGCTTACGATCGTTTTGTCTTACTTGGAGGCATTACTACTACAATCTTGTCATACTTTGCAATAGTAGCCGTGCGAGGCTCCATTATGAAGGCCTTGAAGAAAGATTAACACAACATTTTAGGCAACTAAATGGAATTAGTAAATGAAAAGGCTGAGAAAGATGGGACACTGTATCAATATAGTTCAATCGTTTTTTCTCCGGAAGATTTTGGATGTAGTTGCAGAGGTGGTGTATTGTTTAATTTTGCAACCCTCTGCCTCCCGTTGGTGCAGGGCGGGATCTGATGCCATTGAAGCTTTGCATGTTAGTACGCAAATGATATGATTCTGTATAGCTTCTGTTTCCCGCTTGTAGTTAATCTTACATCAGGTGCAGCATCCACACATGGTTGCTTGCCTGATCATTACCGAAAATCAAACGTCGAGTTGAAGACATTTCTGTATACTTGATTGAACAGTTTATTGGAATTGAAAATAGACAGATAATTGAACCATTCTTGTAATTGCACAGGTTCACTGTTCGCCGATATTATCTTTTTTGGACTTTCCCTCTTTAAAACACGTCTGCTTAAATTTCAGCCGTTTAGCCTTCTTCTCCTCCATAGTCTGAATATAGAA

mRNA sequence

TATTTATTTTGAGGGCTTTCCTTTTACCCGTTCTTTCCACTCCCGATGATCAAATTTCAGTTCAATTTCCCAACTCTACGTGGGCTCTCGAGTCCATTGCAGGGACACATTCATCTTCGTCTTGCGCAGAGCTGAGGTCCCCAAGAACGCTCCAATAGACTGCGATTCGAATCAAAGAATAGCCAAATCAGCATCCATGTCTTTCCATATTCTTCACCTCCTCCTGTTTTTAACTTCTGTTCGTCTCTGTTTATCAGATGAACACTCAATGGAATCGGTTCCTGATCTTCAAAATTCAATGTACCTTGTAGTTGATGGTTATCCGTGCATTCGATTACTCAATCTCTCTGGAGAAATCGGTTGTTCAAATCCTGGGCGAGAAAAGGTTGTAGTACCCATGATTAACTTCAAAGATGCTGATGAGATATTGCAACCGTCTGCAATTTTAGTTTCAATGGATGACATCTCGAGTTTCTTTGCTAGATTACAGGATGATTCCAATTTTGCAAGTAATGTTGGTGGTGTTTTAATCAAACCGGGGACTGAAATACAGAAAAGAACGAAAGGGTTTTCTCCTGCTCAAAAGTTTCCACAAGCTAAATTTGCTCCTTACCAAAAAATTGACTATGAATGGAACCCAATTGGATCTGGAGTTATGTGGAATCAATATAACTTTCCTGTTTTCTTAATATCTGAGAGCAGCATTTCCCCTGTACAGGAGGCTGCTTCAAAAAATGTGAAGGACAAGAAAGTTTACACGTCTAATGTTGCTGAATTTGATCTGGTGATGCAGACAACCAAGGCTGGAACTCGTAATTCAATGTCTTGTTTGAAAGAAGAAACATGCCTTCCGTTAGGTGGATACAGTGTCTGGTCGTCGCTTCCTCCAATCAATATTTCTTCAGATAAGTCTAAGCCCATCATTCTCACAGTAGCTTCAATGGACTCTGCGTCTTTCTTCCGTGATAAAAGCATTGGTGCCGACTCCCCCATCTCTGGCCTGATTGCATTGCTGGCTGCGGTTGATGCACTTTCCCATGTGGATGGACTGGATGATCTTCATAAACAGCTTGTTTTTGTTGTCTTCACTGGAGAGTCTTGGGGCTACCTTGGTAGTAGGAGATTTTTGCTTGAACTTGATTTACAGTCCGATTCTGTCAGTGGCCTTAACAATACATTGATCGATACGGTTTTCGAAATTGGCTCTGTTGGAAAGAAATCCAATGATGGATTTGGAAACTTCTTCGCTCACATGACAGAGGTTTCATCTTCCAAGAATGAGACATGGAACGCCTTGAAGCTTGCTCGAGAGTCGCTTCCATTTGAGAACATAAAAGTCTCACCAGCTAGTACTACAAATCCAGGGATACCACCATCTTCGTTGATGGCATTTCTAGCCAAGAACTCGCAGGTCTCTGGGGTGGTATTAGAAGACTTTGATACTAGCTTTACCAATCAATTTTACCAGAGTCACCTCGACGATTTACATAATATAAACTCATCAGCTATTGAAGCAGCTGCTTTACTTGTTGCCCGAACTCTTTACATTCTTGCAACCAACAAAAAGGAATTGAGTAGTTCTGCCCTGAATGCTATCAAATTGAACACCTCGTTGGTTGAAGAGCTTATAGGATGTCTCCTGAACTGTGACCCTGGCCTCTCTTGTGAGTTGGTGAAGAGATATATTTCTCCGACCAATGTCTGTCCAAACCATTATGTTGGTGTTATCCTCGATGAACCATCCTCTACTCCTTATCCTGGTTATGTTCACGATGTTTCAAGATTTGCTTGGAACTTTTTAGCTGACAGAACATCCATCCGTAAAGAGAATACTAGCTCTGTCTGTTCACAGAACTGTGATGACAAAAGTGAGGTGTGCATTGGAGCGGAGACTGGAAAGGGAACTTGTGTTGTATCAACTACCAGGTACGTTCCAGCATACTCAACACGATTGACGTTCGATTCTGGATCTTGGAATGTGCTTCCTCCGAATGCATCGGACCCAATGGGCGCTGTCGATCCTGTTTGGACAGAGAGCAACTGGAACACCATAGGACTCCGAATGTATACCGTCCAAGCTACTGCTTACGATCGTTTTGTCTTACTTGGAGGCATTACTACTACAATCTTGTCATACTTTGCAATAGTAGCCGTGCGAGGCTCCATTATGAAGGCCTTGAAGAAAGATTAACACAACATTTTAGGCAACTAAATGGAATTAGTAAATGAAAAGGCTGAGAAAGATGGGACACTGTATCAATATAGTTCAATCGTTTTTTCTCCGGAAGATTTTGGATGTAGTTGCAGAGGTGGTGTATTGTTTAATTTTGCAACCCTCTGCCTCCCGTTGGTGCAGGGCGGGATCTGATGCCATTGAAGCTTTGCATGTTAGTACAGCTTCTGTTTCCCGCTTGTAGTTAATCTTACATCAGGTGCAGCATCCACACATGGTTGCTTGCCTGATCATTACCGAAAATCAAACGTCGAGTTGAAGACATTTCTGTATACTTGATTGAACAGTTTATTGGAATTGAAAATAGACAGATAATTGAACCATTCTTGTAATTGCACAGGTTCACTGTTCGCCGATATTATCTTTTTTGGACTTTCCCTCTTTAAAACACGTCTGCTTAAATTTCAGCCGTTTAGCCTTCTTCTCCTCCATAGTCTGAATATAGAA

Coding sequence (CDS)

ATGTCTTTCCATATTCTTCACCTCCTCCTGTTTTTAACTTCTGTTCGTCTCTGTTTATCAGATGAACACTCAATGGAATCGGTTCCTGATCTTCAAAATTCAATGTACCTTGTAGTTGATGGTTATCCGTGCATTCGATTACTCAATCTCTCTGGAGAAATCGGTTGTTCAAATCCTGGGCGAGAAAAGGTTGTAGTACCCATGATTAACTTCAAAGATGCTGATGAGATATTGCAACCGTCTGCAATTTTAGTTTCAATGGATGACATCTCGAGTTTCTTTGCTAGATTACAGGATGATTCCAATTTTGCAAGTAATGTTGGTGGTGTTTTAATCAAACCGGGGACTGAAATACAGAAAAGAACGAAAGGGTTTTCTCCTGCTCAAAAGTTTCCACAAGCTAAATTTGCTCCTTACCAAAAAATTGACTATGAATGGAACCCAATTGGATCTGGAGTTATGTGGAATCAATATAACTTTCCTGTTTTCTTAATATCTGAGAGCAGCATTTCCCCTGTACAGGAGGCTGCTTCAAAAAATGTGAAGGACAAGAAAGTTTACACGTCTAATGTTGCTGAATTTGATCTGGTGATGCAGACAACCAAGGCTGGAACTCGTAATTCAATGTCTTGTTTGAAAGAAGAAACATGCCTTCCGTTAGGTGGATACAGTGTCTGGTCGTCGCTTCCTCCAATCAATATTTCTTCAGATAAGTCTAAGCCCATCATTCTCACAGTAGCTTCAATGGACTCTGCGTCTTTCTTCCGTGATAAAAGCATTGGTGCCGACTCCCCCATCTCTGGCCTGATTGCATTGCTGGCTGCGGTTGATGCACTTTCCCATGTGGATGGACTGGATGATCTTCATAAACAGCTTGTTTTTGTTGTCTTCACTGGAGAGTCTTGGGGCTACCTTGGTAGTAGGAGATTTTTGCTTGAACTTGATTTACAGTCCGATTCTGTCAGTGGCCTTAACAATACATTGATCGATACGGTTTTCGAAATTGGCTCTGTTGGAAAGAAATCCAATGATGGATTTGGAAACTTCTTCGCTCACATGACAGAGGTTTCATCTTCCAAGAATGAGACATGGAACGCCTTGAAGCTTGCTCGAGAGTCGCTTCCATTTGAGAACATAAAAGTCTCACCAGCTAGTACTACAAATCCAGGGATACCACCATCTTCGTTGATGGCATTTCTAGCCAAGAACTCGCAGGTCTCTGGGGTGGTATTAGAAGACTTTGATACTAGCTTTACCAATCAATTTTACCAGAGTCACCTCGACGATTTACATAATATAAACTCATCAGCTATTGAAGCAGCTGCTTTACTTGTTGCCCGAACTCTTTACATTCTTGCAACCAACAAAAAGGAATTGAGTAGTTCTGCCCTGAATGCTATCAAATTGAACACCTCGTTGGTTGAAGAGCTTATAGGATGTCTCCTGAACTGTGACCCTGGCCTCTCTTGTGAGTTGGTGAAGAGATATATTTCTCCGACCAATGTCTGTCCAAACCATTATGTTGGTGTTATCCTCGATGAACCATCCTCTACTCCTTATCCTGGTTATGTTCACGATGTTTCAAGATTTGCTTGGAACTTTTTAGCTGACAGAACATCCATCCGTAAAGAGAATACTAGCTCTGTCTGTTCACAGAACTGTGATGACAAAAGTGAGGTGTGCATTGGAGCGGAGACTGGAAAGGGAACTTGTGTTGTATCAACTACCAGGTACGTTCCAGCATACTCAACACGATTGACGTTCGATTCTGGATCTTGGAATGTGCTTCCTCCGAATGCATCGGACCCAATGGGCGCTGTCGATCCTGTTTGGACAGAGAGCAACTGGAACACCATAGGACTCCGAATGTATACCGTCCAAGCTACTGCTTACGATCGTTTTGTCTTACTTGGAGGCATTACTACTACAATCTTGTCATACTTTGCAATAGTAGCCGTGCGAGGCTCCATTATGAAGGCCTTGAAGAAAGATTAA

Protein sequence

MSFHILHLLLFLTSVRLCLSDEHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPGREKVVVPMINFKDADEILQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQKRTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKNVKDKKVYTSNVAEFDLVMQTTKAGTRNSMSCLKEETCLPLGGYSVWSSLPPINISSDKSKPIILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGESWGYLGSRRFLLELDLQSDSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSKNETWNALKLARESLPFENIKVSPASTTNPGIPPSSLMAFLAKNSQVSGVVLEDFDTSFTNQFYQSHLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEELIGCLLNCDPGLSCELVKRYISPTNVCPNHYVGVILDEPSSTPYPGYVHDVSRFAWNFLADRTSIRKENTSSVCSQNCDDKSEVCIGAETGKGTCVVSTTRYVPAYSTRLTFDSGSWNVLPPNASDPMGAVDPVWTESNWNTIGLRMYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKALKKD
Homology
BLAST of Carg07431 vs. NCBI nr
Match: KAG7036691.1 (Nicastrin [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1315.4 bits (3403), Expect = 0.0e+00
Identity = 664/664 (100.00%), Postives = 664/664 (100.00%), Query Frame = 0

Query: 1   MSFHILHLLLFLTSVRLCLSDEHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPG 60
           MSFHILHLLLFLTSVRLCLSDEHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPG
Sbjct: 1   MSFHILHLLLFLTSVRLCLSDEHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPG 60

Query: 61  REKVVVPMINFKDADEILQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQK 120
           REKVVVPMINFKDADEILQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQK
Sbjct: 61  REKVVVPMINFKDADEILQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQK 120

Query: 121 RTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKN 180
           RTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKN
Sbjct: 121 RTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKN 180

Query: 181 VKDKKVYTSNVAEFDLVMQTTKAGTRNSMSCLKEETCLPLGGYSVWSSLPPINISSDKSK 240
           VKDKKVYTSNVAEFDLVMQTTKAGTRNSMSCLKEETCLPLGGYSVWSSLPPINISSDKSK
Sbjct: 181 VKDKKVYTSNVAEFDLVMQTTKAGTRNSMSCLKEETCLPLGGYSVWSSLPPINISSDKSK 240

Query: 241 PIILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGE 300
           PIILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGE
Sbjct: 241 PIILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGE 300

Query: 301 SWGYLGSRRFLLELDLQSDSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSK 360
           SWGYLGSRRFLLELDLQSDSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSK
Sbjct: 301 SWGYLGSRRFLLELDLQSDSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSK 360

Query: 361 NETWNALKLARESLPFENIKVSPASTTNPGIPPSSLMAFLAKNSQVSGVVLEDFDTSFTN 420
           NETWNALKLARESLPFENIKVSPASTTNPGIPPSSLMAFLAKNSQVSGVVLEDFDTSFTN
Sbjct: 361 NETWNALKLARESLPFENIKVSPASTTNPGIPPSSLMAFLAKNSQVSGVVLEDFDTSFTN 420

Query: 421 QFYQSHLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEELIGC 480
           QFYQSHLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEELIGC
Sbjct: 421 QFYQSHLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEELIGC 480

Query: 481 LLNCDPGLSCELVKRYISPTNVCPNHYVGVILDEPSSTPYPGYVHDVSRFAWNFLADRTS 540
           LLNCDPGLSCELVKRYISPTNVCPNHYVGVILDEPSSTPYPGYVHDVSRFAWNFLADRTS
Sbjct: 481 LLNCDPGLSCELVKRYISPTNVCPNHYVGVILDEPSSTPYPGYVHDVSRFAWNFLADRTS 540

Query: 541 IRKENTSSVCSQNCDDKSEVCIGAETGKGTCVVSTTRYVPAYSTRLTFDSGSWNVLPPNA 600
           IRKENTSSVCSQNCDDKSEVCIGAETGKGTCVVSTTRYVPAYSTRLTFDSGSWNVLPPNA
Sbjct: 541 IRKENTSSVCSQNCDDKSEVCIGAETGKGTCVVSTTRYVPAYSTRLTFDSGSWNVLPPNA 600

Query: 601 SDPMGAVDPVWTESNWNTIGLRMYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKA 660
           SDPMGAVDPVWTESNWNTIGLRMYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKA
Sbjct: 601 SDPMGAVDPVWTESNWNTIGLRMYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKA 660

Query: 661 LKKD 665
           LKKD
Sbjct: 661 LKKD 664

BLAST of Carg07431 vs. NCBI nr
Match: XP_022948878.1 (nicastrin [Cucurbita moschata])

HSP 1 Score: 1310.4 bits (3390), Expect = 0.0e+00
Identity = 660/664 (99.40%), Postives = 664/664 (100.00%), Query Frame = 0

Query: 1   MSFHILHLLLFLTSVRLCLSDEHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPG 60
           MSFHILHLLLFLTSVRLCLSDEHSMESVPDLQNSMYLVVDG+PCIRLLNLSGEIGCSNPG
Sbjct: 1   MSFHILHLLLFLTSVRLCLSDEHSMESVPDLQNSMYLVVDGHPCIRLLNLSGEIGCSNPG 60

Query: 61  REKVVVPMINFKDADEILQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQK 120
           REKVVVPMINFKDADEILQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQK
Sbjct: 61  REKVVVPMINFKDADEILQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQK 120

Query: 121 RTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKN 180
           RTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKN
Sbjct: 121 RTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKN 180

Query: 181 VKDKKVYTSNVAEFDLVMQTTKAGTRNSMSCLKEETCLPLGGYSVWSSLPPINISSDKSK 240
           VKDKK+YTSNVAEFDLVMQTTKAGTRNSMSCLKEETCLPLGGYSVWSSLPPINISSDKSK
Sbjct: 181 VKDKKIYTSNVAEFDLVMQTTKAGTRNSMSCLKEETCLPLGGYSVWSSLPPINISSDKSK 240

Query: 241 PIILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGE 300
           PIILTVASMDSASFFRDKSIGADSPISGLIALLA+VDALSHVDGLDDLHKQLVFVVFTGE
Sbjct: 241 PIILTVASMDSASFFRDKSIGADSPISGLIALLASVDALSHVDGLDDLHKQLVFVVFTGE 300

Query: 301 SWGYLGSRRFLLELDLQSDSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSK 360
           SWGYLGSRRFLLELDLQSDSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSK
Sbjct: 301 SWGYLGSRRFLLELDLQSDSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSK 360

Query: 361 NETWNALKLARESLPFENIKVSPASTTNPGIPPSSLMAFLAKNSQVSGVVLEDFDTSFTN 420
           NETWNALKLA+ESLPFENIKVSPASTTNPGIPPSSLMAFLAKNSQVSGVVLEDFDTSFTN
Sbjct: 361 NETWNALKLAQESLPFENIKVSPASTTNPGIPPSSLMAFLAKNSQVSGVVLEDFDTSFTN 420

Query: 421 QFYQSHLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEELIGC 480
           QFYQSHLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEELIGC
Sbjct: 421 QFYQSHLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEELIGC 480

Query: 481 LLNCDPGLSCELVKRYISPTNVCPNHYVGVILDEPSSTPYPGYVHDVSRFAWNFLADRTS 540
           LLNCDPGLSCELVKRYISPTNVCPNHYVGVILDEPSSTPYPGYVHDVSRFAWNFLADRTS
Sbjct: 481 LLNCDPGLSCELVKRYISPTNVCPNHYVGVILDEPSSTPYPGYVHDVSRFAWNFLADRTS 540

Query: 541 IRKENTSSVCSQNCDDKSEVCIGAETGKGTCVVSTTRYVPAYSTRLTFDSGSWNVLPPNA 600
           IRKENTSSVCSQNCDDKSEVCIGAETGKGTCVVSTTRYVPAYSTRLTFDSGSWNVLPPNA
Sbjct: 541 IRKENTSSVCSQNCDDKSEVCIGAETGKGTCVVSTTRYVPAYSTRLTFDSGSWNVLPPNA 600

Query: 601 SDPMGAVDPVWTESNWNTIGLRMYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKA 660
           SDPMGAVDPVWTESNWNTIGLRMYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKA
Sbjct: 601 SDPMGAVDPVWTESNWNTIGLRMYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKA 660

Query: 661 LKKD 665
           LKKD
Sbjct: 661 LKKD 664

BLAST of Carg07431 vs. NCBI nr
Match: KAG6606989.1 (Nicastrin, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1309.7 bits (3388), Expect = 0.0e+00
Identity = 661/664 (99.55%), Postives = 663/664 (99.85%), Query Frame = 0

Query: 1   MSFHILHLLLFLTSVRLCLSDEHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPG 60
           MSFHILHLLLFLTSVRLCLSDEHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPG
Sbjct: 46  MSFHILHLLLFLTSVRLCLSDEHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPG 105

Query: 61  REKVVVPMINFKDADEILQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQK 120
           REKVVVPMINFKDADEILQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQK
Sbjct: 106 REKVVVPMINFKDADEILQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQK 165

Query: 121 RTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKN 180
           RTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKN
Sbjct: 166 RTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKN 225

Query: 181 VKDKKVYTSNVAEFDLVMQTTKAGTRNSMSCLKEETCLPLGGYSVWSSLPPINISSDKSK 240
           VKDKK+YTSNVAEFDLVMQTTKAGTRNSMSCLKEETCLPLGGYSVWSSLPPINISSDKSK
Sbjct: 226 VKDKKIYTSNVAEFDLVMQTTKAGTRNSMSCLKEETCLPLGGYSVWSSLPPINISSDKSK 285

Query: 241 PIILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGE 300
           PIILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGE
Sbjct: 286 PIILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGE 345

Query: 301 SWGYLGSRRFLLELDLQSDSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSK 360
           SWGYLGSRRFLLELDLQSDSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSK
Sbjct: 346 SWGYLGSRRFLLELDLQSDSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSK 405

Query: 361 NETWNALKLARESLPFENIKVSPASTTNPGIPPSSLMAFLAKNSQVSGVVLEDFDTSFTN 420
           NETWNALKLA+ESLPFENIKVSPASTTNPGIPPSSLMAFLAKNSQVSGVVLEDFDTSFTN
Sbjct: 406 NETWNALKLAQESLPFENIKVSPASTTNPGIPPSSLMAFLAKNSQVSGVVLEDFDTSFTN 465

Query: 421 QFYQSHLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEELIGC 480
           QFYQSHLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEELIGC
Sbjct: 466 QFYQSHLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEELIGC 525

Query: 481 LLNCDPGLSCELVKRYISPTNVCPNHYVGVILDEPSSTPYPGYVHDVSRFAWNFLADRTS 540
           LLNCDPGLSCELVKRYISPTNVCPNHYVGVILDEPSSTPYPGYVHDVSRFAWNFLADRTS
Sbjct: 526 LLNCDPGLSCELVKRYISPTNVCPNHYVGVILDEPSSTPYPGYVHDVSRFAWNFLADRTS 585

Query: 541 IRKENTSSVCSQNCDDKSEVCIGAETGKGTCVVSTTRYVPAYSTRLTFDSGSWNVLPPNA 600
           IRKENTSSVCSQNCDDKSEVCIGAETGKGTCVVSTTRYVPAYSTRLTFDSGSWNVLP NA
Sbjct: 586 IRKENTSSVCSQNCDDKSEVCIGAETGKGTCVVSTTRYVPAYSTRLTFDSGSWNVLPLNA 645

Query: 601 SDPMGAVDPVWTESNWNTIGLRMYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKA 660
           SDPMGAVDPVWTESNWNTIGLRMYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKA
Sbjct: 646 SDPMGAVDPVWTESNWNTIGLRMYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKA 705

Query: 661 LKKD 665
           LKKD
Sbjct: 706 LKKD 709

BLAST of Carg07431 vs. NCBI nr
Match: XP_023525268.1 (nicastrin [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1306.2 bits (3379), Expect = 0.0e+00
Identity = 658/664 (99.10%), Postives = 662/664 (99.70%), Query Frame = 0

Query: 1   MSFHILHLLLFLTSVRLCLSDEHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPG 60
           MSFH+LHLLLFLTSVRLCLSDEHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPG
Sbjct: 1   MSFHVLHLLLFLTSVRLCLSDEHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPG 60

Query: 61  REKVVVPMINFKDADEILQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQK 120
           REKVVVPMINFKDADEILQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQK
Sbjct: 61  REKVVVPMINFKDADEILQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQK 120

Query: 121 RTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKN 180
           RTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKN
Sbjct: 121 RTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKN 180

Query: 181 VKDKKVYTSNVAEFDLVMQTTKAGTRNSMSCLKEETCLPLGGYSVWSSLPPINISSDKSK 240
           VKDKKVYTSNVAEFDLVMQTTKAGT NSMSCLKEETCLPLGGYSVWSSLPPINISSDKSK
Sbjct: 181 VKDKKVYTSNVAEFDLVMQTTKAGTHNSMSCLKEETCLPLGGYSVWSSLPPINISSDKSK 240

Query: 241 PIILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGE 300
           PIILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGE
Sbjct: 241 PIILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGE 300

Query: 301 SWGYLGSRRFLLELDLQSDSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSK 360
           SWGYLGSRRFLLELDLQS+SVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSK
Sbjct: 301 SWGYLGSRRFLLELDLQSNSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSK 360

Query: 361 NETWNALKLARESLPFENIKVSPASTTNPGIPPSSLMAFLAKNSQVSGVVLEDFDTSFTN 420
           NETWNALKLARESLPFENIKVSPASTTNPGIPPSSLMAFLAKNSQVSGVVLEDFDTSFTN
Sbjct: 361 NETWNALKLARESLPFENIKVSPASTTNPGIPPSSLMAFLAKNSQVSGVVLEDFDTSFTN 420

Query: 421 QFYQSHLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEELIGC 480
           QFYQSHLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEELIGC
Sbjct: 421 QFYQSHLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEELIGC 480

Query: 481 LLNCDPGLSCELVKRYISPTNVCPNHYVGVILDEPSSTPYPGYVHDVSRFAWNFLADRTS 540
           LLNCDPGLSCELVKRYISPTNVCPNHYVGVILDEPSSTPYPGYVHDVSRFAWNFLADRTS
Sbjct: 481 LLNCDPGLSCELVKRYISPTNVCPNHYVGVILDEPSSTPYPGYVHDVSRFAWNFLADRTS 540

Query: 541 IRKENTSSVCSQNCDDKSEVCIGAETGKGTCVVSTTRYVPAYSTRLTFDSGSWNVLPPNA 600
           IRKENTSSVCSQNCDDKSEVCIGAETGKGTCVVSTTRYVPAYSTRL F+SGSWNVLPPN+
Sbjct: 541 IRKENTSSVCSQNCDDKSEVCIGAETGKGTCVVSTTRYVPAYSTRLMFESGSWNVLPPNS 600

Query: 601 SDPMGAVDPVWTESNWNTIGLRMYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKA 660
           SDPMGAVDPVWTESNWNTIGLRMYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKA
Sbjct: 601 SDPMGAVDPVWTESNWNTIGLRMYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKA 660

Query: 661 LKKD 665
           LKKD
Sbjct: 661 LKKD 664

BLAST of Carg07431 vs. NCBI nr
Match: XP_022997757.1 (nicastrin [Cucurbita maxima])

HSP 1 Score: 1290.8 bits (3339), Expect = 0.0e+00
Identity = 650/664 (97.89%), Postives = 655/664 (98.64%), Query Frame = 0

Query: 1   MSFHILHLLLFLTSVRLCLSDEHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPG 60
           M+FHILHLLLFLTS RLCLSDEHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPG
Sbjct: 1   MAFHILHLLLFLTSARLCLSDEHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPG 60

Query: 61  REKVVVPMINFKDADEILQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQK 120
           REKVVVPMINFKDADEI QPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQK
Sbjct: 61  REKVVVPMINFKDADEIFQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQK 120

Query: 121 RTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKN 180
           RTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKN
Sbjct: 121 RTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKN 180

Query: 181 VKDKKVYTSNVAEFDLVMQTTKAGTRNSMSCLKEETCLPLGGYSVWSSLPPINISSDKSK 240
           VKDKKVYTSNVAEFDLVMQTTKAGT NSMSCLKEETCLPLGGYSVWSSLPPINIS DKSK
Sbjct: 181 VKDKKVYTSNVAEFDLVMQTTKAGTHNSMSCLKEETCLPLGGYSVWSSLPPINISLDKSK 240

Query: 241 PIILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGE 300
           PIILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGE
Sbjct: 241 PIILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGE 300

Query: 301 SWGYLGSRRFLLELDLQSDSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSK 360
           SWGYLGSRRFLLELDLQSDSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSK
Sbjct: 301 SWGYLGSRRFLLELDLQSDSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSK 360

Query: 361 NETWNALKLARESLPFENIKVSPASTTNPGIPPSSLMAFLAKNSQVSGVVLEDFDTSFTN 420
           NETWNALKLA+ESLPFENIKVSPASTTNPGIPPSSLMAFLAKNSQVSGVVLEDFDTSFTN
Sbjct: 361 NETWNALKLAQESLPFENIKVSPASTTNPGIPPSSLMAFLAKNSQVSGVVLEDFDTSFTN 420

Query: 421 QFYQSHLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEELIGC 480
           QFYQSHLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEE+IGC
Sbjct: 421 QFYQSHLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEEIIGC 480

Query: 481 LLNCDPGLSCELVKRYISPTNVCPNHYVGVILDEPSSTPYPGYVHDVSRFAWNFLADRTS 540
           LLNCDPGLSCELVKRYISP NVCPNHYVGVILDEPSSTPYPGYVHDVSRF WNFLADRTS
Sbjct: 481 LLNCDPGLSCELVKRYISPINVCPNHYVGVILDEPSSTPYPGYVHDVSRFVWNFLADRTS 540

Query: 541 IRKENTSSVCSQNCDDKSEVCIGAETGKGTCVVSTTRYVPAYSTRLTFDSGSWNVLPPNA 600
           I KENTSSVCSQNCDDKSEVCIGAETGKGTCVVSTTRYVPAYSTRL F+SGSWNVLPPN+
Sbjct: 541 IPKENTSSVCSQNCDDKSEVCIGAETGKGTCVVSTTRYVPAYSTRLMFESGSWNVLPPNS 600

Query: 601 SDPMGAVDPVWTESNWNTIGLRMYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKA 660
           SDPMGAVDPVWTESNWNTIGLR YTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKA
Sbjct: 601 SDPMGAVDPVWTESNWNTIGLRTYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKA 660

Query: 661 LKKD 665
           LKKD
Sbjct: 661 LKKD 664

BLAST of Carg07431 vs. ExPASy Swiss-Prot
Match: Q8GUM5 (Nicastrin OS=Arabidopsis thaliana OX=3702 GN=At3g52640/At3g52650 PE=2 SV=1)

HSP 1 Score: 844.0 bits (2179), Expect = 1.2e-243
Identity = 421/661 (63.69%), Postives = 519/661 (78.52%), Query Frame = 0

Query: 8   LLLFLTSVRLCLSDE-HSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPGREKVVV 67
           +LL +  + L L+DE  S+ESVPDLQ  MY+ VDG+PC+RLLNLSGEIGCSNPG  KVV 
Sbjct: 16  VLLSILPLHLSLADEITSIESVPDLQKLMYVAVDGFPCVRLLNLSGEIGCSNPGINKVVA 75

Query: 68  PMINFKDADEILQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQKRTKGFS 127
           P+I  KD  +++QP  ILV+ D++  FF R+  D +FAS +GGVL++ G+  Q++ KGFS
Sbjct: 76  PIIKLKDVKDLVQPHTILVTADEMEDFFTRVSTDLSFASKIGGVLVESGSNFQQKLKGFS 135

Query: 128 PAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKNVKDKKV 187
           P ++FPQA+F+PY+ ++Y+WN   S +MW  YNFPV+L+SES IS V E  SK       
Sbjct: 136 PDKRFPQAQFSPYENVEYKWNSAASSIMWRNYNFPVYLLSESGISAVHEILSKKKMKHGT 195

Query: 188 YTSNVAEFDLVMQTTKAGTRNSMSCLKEETCLPLGGYSVWSSLPPINISSDKS-KPIILT 247
           YTS+VAEF++VM+TTKAGT NS +CL+E TCLPLGGYSVWSSLPPI++SS  + KP++LT
Sbjct: 196 YTSDVAEFNMVMETTKAGTHNSEACLQEGTCLPLGGYSVWSSLPPISVSSSNNRKPVVLT 255

Query: 248 VASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGESWGYL 307
           VASMD+ASFFRDKS GADSPISGL+ALL AVDALS VDG+ +L KQLVF+V TGE+WGYL
Sbjct: 256 VASMDTASFFRDKSFGADSPISGLVALLGAVDALSRVDGISNLKKQLVFLVLTGETWGYL 315

Query: 308 GSRRFLLELDLQSDSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSKNETWN 367
           GSRRFL ELDL SD+V+GL+NT I+TV EIGSVGK  + G   FFAH T VSS  N T +
Sbjct: 316 GSRRFLHELDLHSDAVAGLSNTSIETVLEIGSVGKGLSGGINTFFAHKTRVSSVTNMTLD 375

Query: 368 ALKLARESLPFENIKVSPASTTNPGIPPSSLMAFLAKNSQVSGVVLEDFDTSFTNQFYQS 427
           ALK+A++SL  +NIK+  A T NPGIPPSSLMAF+ KN Q S VVLEDFDT+F N+FY S
Sbjct: 376 ALKIAQDSLASKNIKILSADTANPGIPPSSLMAFMRKNPQTSAVVLEDFDTNFVNKFYHS 435

Query: 428 HLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEELIGCLLNCD 487
           HLDDL NINSS++ AAA +VARTLYILA++ K+ S+SAL +I +N S VEEL+ CLL C+
Sbjct: 436 HLDDLSNINSSSVVAAASVVARTLYILASDNKDTSNSALGSIHVNASFVEELLTCLLACE 495

Query: 488 PGLSCELVKRYISPTNVCPNHYVGVILDEPSSTPYPGYVHDVSRFAWNFLADRTSIRKEN 547
           PGLSC LVK YISPTN CP +Y GVIL EPSS PY GYV DVSRF WNFLAD+TS++K N
Sbjct: 496 PGLSCNLVKDYISPTNTCPGNYAGVILGEPSSKPYLGYVGDVSRFLWNFLADKTSVQKGN 555

Query: 548 TSSVCSQN-CDDKSEVCIGAETGK-GTCVVSTTRYVPAYSTRLTFDSGSWNVLPPNASDP 607
           T+SVCS+  C    EVCI AE+ K GTCVVSTTRYVPAYSTRL ++ G+W +LP N+SD 
Sbjct: 556 TTSVCSKGVCSKTDEVCIKAESNKEGTCVVSTTRYVPAYSTRLKYNDGAWTILPQNSSDS 615

Query: 608 MGAVDPVWTESNWNTIGLRMYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKALKK 665
           MG VDPVWTESNW+T+ + +YTVQ +AYD  VL+ GIT T L+Y  I+A +  I KALK+
Sbjct: 616 MGMVDPVWTESNWDTLRVHVYTVQHSAYDNAVLVAGITVTTLAYIGILAAKSIITKALKQ 675

BLAST of Carg07431 vs. ExPASy Swiss-Prot
Match: F0ZBA6 (Nicastrin OS=Dictyostelium purpureum OX=5786 GN=ncstn PE=1 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 2.6e-49
Identity = 175/681 (25.70%), Postives = 307/681 (45.08%), Query Frame = 0

Query: 1   MSFHILHLLLFLTSVRLCLSDEHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCS--- 60
           M F  + +LL L    +  S+  +  ++ D  N    +   YPC +++   G+ GCS   
Sbjct: 1   MRFKNVLVLLLLLVFSVINSEPSAPATISD--NIYTTLYSSYPCTKIMTSDGQFGCSSKH 60

Query: 61  --NPGREKVVVPMINFKDADEILQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPG 120
             N G   ++    ++ +     Q   I+V + D + F +    + +  S + G+++   
Sbjct: 61  GGNSGILYLIDDDESYNNYFSYSQQKDIIVVL-DTNYFNSTSVLNLHNKSKIEGIIVLTD 120

Query: 121 TEIQKRTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQE 180
           T   K+T  +SP  ++P   +  Y   + EWNP   G  +  + FP+F I   +   ++ 
Sbjct: 121 T---KKTYPYSPDSRYPNKIYGLYPNSNLEWNPNADGFTYFSFPFPIFAIDNQTSVAIRN 180

Query: 181 AASKNVKDKKVYTSNVAEFDLVMQTTKAGTRNSMSCLKEETCLPLGGYSVWSSLPPINIS 240
             SK+ +D + Y +  AE D  MQ    G  NS +CL+   C P+GG S+WSS    +  
Sbjct: 181 -VSKHNRDGQ-YPAWGAELDSFMQ----GAINSETCLRRGFCEPVGGQSIWSS---FSSK 240

Query: 241 SDKSKPIILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFV 300
            DK K IIL +   D+ +FFRD SIGAD      + LL+ + +L+ VD     +K++VF 
Sbjct: 241 IDKEKEIILVMLPFDTTAFFRDLSIGADQSSFATVTLLSVIKSLAAVD-RSSWNKEVVFA 300

Query: 301 VFTGESWGYLGSRRFLLEL------DLQSDS-------------VSGLNNTLIDTVFEIG 360
            +  E WGY+GS  F+ +L         SD               + +N T I T+ E+ 
Sbjct: 301 FWNAERWGYVGSEYFINDLLNFQCKTYNSDKSKCIDPPRADLAFQTQINFTKISTIIELN 360

Query: 361 SVGKKSND-GFGNFFAHMTEVSSSKNETWNALKLARESLPFENIKVSPASTTNPGIPPSS 420
            +G+   D   G +  ++    +  +   + L     S  +EN  ++   TT   +PPSS
Sbjct: 361 QIGRAQLDKNLGKYSLYLHTAGTKTSSVTDILDQVASS--YENSTITFKPTTQTELPPSS 420

Query: 421 LMAFLAKNSQVSGVVLEDFDTSFTNQFYQSHLDDLHNINSSAIEAAALLVARTLYILATN 480
            M+FL K +++  VV+ D D  ++N +Y    DD  N+  S +          +YIL+T 
Sbjct: 421 SMSFLKKTNKIPVVVITDHDYKYSNPYYGYEQDDNENVLGSTLN-------DIVYILSTF 480

Query: 481 KKELSSSALNAIKLNTSLVEELIGCLLNCDPGLSCELVKRYISPTNVCPNHYVGVILDEP 540
              ++    N I ++ + +  L  C  +    ++C  +     P N  PN Y  V     
Sbjct: 481 IDRIAGGN-NNITIDKNFINILYPCFTS---SITCFNILMKTYPLNEVPNFYSSVFGTSL 540

Query: 541 SSTPYPGYVHDVSRFAWNFLADRTSIRKENTSSVCSQNCDDKSEVCIGAETGKGTCVVST 600
           ++T  P     + R  ++        +  +T + C+ + D  S +C       G CV S 
Sbjct: 541 TTTLSPYETKLIHRLLYSI------TQYNSTLTNCTSDNDCPSSLCY-----SGQCVSSN 600

Query: 601 TRYVPAYSTRLTFDSGS--WNVLPPNASDPMGAVDPVWTESNWNTIGLRMYTVQATAYDR 655
           T    A S    FD+    W ++  N+S       P++TESNW+   L+++ +  +  + 
Sbjct: 601 THLHNALSLGFDFDTSKNVWKIV--NSS------YPIFTESNWDYTALKVFKIGNSTTEI 633

BLAST of Carg07431 vs. ExPASy Swiss-Prot
Match: Q54JT7 (Nicastrin OS=Dictyostelium discoideum OX=44689 GN=ncstn PE=3 SV=2)

HSP 1 Score: 196.1 bits (497), Expect = 1.3e-48
Identity = 181/687 (26.35%), Postives = 314/687 (45.71%), Query Frame = 0

Query: 25  MESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNP-GREKVVVPMINFKDADEIL----- 84
           + S   +++ MY  ++ YPC R++ L+G+IGCS+  G +  ++ +I   D+DE       
Sbjct: 21  ISSQSSIEDKMYTSLNSYPCTRIMTLNGQIGCSSSHGGDSGILYLI---DSDESYHNYFS 80

Query: 85  --QPSAILVSMDDISSFFAR-LQDDSNFASNVGGVLIKPGTEIQKRTKGFSPAQKFPQAK 144
             Q   I+V  D  S++F + L  +      + G L+   T+I K T  +SP  ++P  +
Sbjct: 81  YNQQKDIIVVFD--SNYFNKTLVLEMYSKKKMNGALVL--TDIGK-TYPYSPEDQYPIKQ 140

Query: 145 FAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKNVKDKKVYTSNVAEFD 204
           F  Y   +  WNP G G  +  + FP+F +   +   ++  ++ N +D K Y +  AE D
Sbjct: 141 FGLYPDSNLNWNPNGDGFTYMNFPFPMFALELKTSIIIRNLSTIN-RDGK-YPAYGAELD 200

Query: 205 LVMQTTKAGTRNSMSCLKEETCLPLGGYSVWSSLPPINISSDKSKPIILTVASMDSASFF 264
             MQ    G  N+ +CL+   C P+GG S+WSS   +    D+SKPIIL +  +D+ +FF
Sbjct: 201 SFMQ----GAINAETCLRRGFCEPVGGQSIWSSFSEV---IDQSKPIILVMLPIDATAFF 260

Query: 265 RDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGESWGYLGSRRFLLEL- 324
           RD + G D     L  LL+ ++ L  VD      K+++F ++  E WGY+GS  F+ +L 
Sbjct: 261 RDLATGTDQSGYALTVLLSMLNTLQGVD-KTKWDKEVIFAMWNSERWGYVGSTNFVNDLL 320

Query: 325 -----DLQSDSVSGLNN-TLIDTVF------------EIGSVGKKSNDG--------FGN 384
                 L S++ +  ++  ++D  F            E   +G+  N G          N
Sbjct: 321 NFNCTSLDSNNQNSCSSPPMLDLTFEQIKFENIYAIIEFNQIGRPVNSGKKTPNKLDIYN 380

Query: 385 FFAHMTEVSSSKNETWNALKLARESLPFENIKVSPASTTNPGIPPSSLMAFLAKNSQVSG 444
              H     +  N+  +    + +S  +EN  +    TT   +PP S M+F+ + ++ S 
Sbjct: 381 LVFH-PNGGAGANQLMDVFSQSTQS--YENSTIQFQKTTQNELPPCSSMSFIKEINKKSA 440

Query: 445 ------VVLEDFDTSFTNQFYQSHLDDLHNINSSAIEAAALLVARTLYILA---TNKKEL 504
                 +V+ D D  + N ++    D+  NIN++           TL+ +    +   +L
Sbjct: 441 PNFIGTLVITDHDYQYNNPYFGDEQDNSGNINTT---------TSTLFDMVQVFSKSIDL 500

Query: 505 SSSALNAIKLNTSLVEELIGCLLNCDPGLSCELVKRYIS--PTNVCPNHYVGVILDEPSS 564
            +     +K++   + E+  CL      ++C  V + +S  P N  PN Y GV    P +
Sbjct: 501 LAGGNGTVKVDDLFIREINVCLTQ---SITCNWVTKLMSTFPYNPIPNFYSGVYGVSPVN 560

Query: 565 TPYPGYVHDVSRFAWNFLADRTSIRKENTSSVCSQNCDDKSEVCIGAETGKGTCVVSTTR 624
              P      +RF +      T  R   T+     +CD  S +C+        C+ S T 
Sbjct: 561 HITP----IETRFIFRMATYLTQHRTNATNCTSDNDCDTSSSICVNK-----VCLYSNTH 620

Query: 625 YVPAYSTRLTFDS--GSWNVLPPNASDPMGAVDPVWTESNWNTIGLRMYTVQATAYDRFV 663
           Y  A S   +FD+   SW ++  N S       PV+ ESNW+   +R++ V + A + + 
Sbjct: 621 YHNAISLAFSFDNSKSSWTIV--NTS------YPVFVESNWDYTTVRLFQVGSYANEIWF 657

BLAST of Carg07431 vs. ExPASy Swiss-Prot
Match: Q8CGU6 (Nicastrin OS=Rattus norvegicus OX=10116 GN=Ncstn PE=1 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 7.2e-31
Identity = 166/676 (24.56%), Postives = 288/676 (42.60%), Query Frame = 0

Query: 43  PCIRLLNLSGEIGC-SNPGREKVVVPMINFKD------ADEILQPSAILVSMDDIS-SFF 102
           PC+RLLN + +IGC S+   +  V+ ++  +D       D    P  +L+     +    
Sbjct: 48  PCVRLLNATHQIGCQSSISGDTGVIHVVEKEDDLKWVLTDGPNPPYMVLLEGKLFTRDIM 107

Query: 103 ARLQDDSNFASNVGGVLIKPGTEIQKRTKGFSPAQKFPQAKFAPYQKI---------DYE 162
            +L+ +++  + +   L KP +     T  FSP+ + P   F  Y               
Sbjct: 108 EKLKGETSRIAGLAVTLAKPNS-----TSSFSPSVQCPNDGFGIYSNSYGPEFAHCKKTL 167

Query: 163 WNPIGSGVMWNQYNFPVFLISESSISPV-------------QEAASKNVKDKKVYTSNVA 222
           WN +G+G+ ++ ++FP+FL+ + + + V               A S  +   ++++   A
Sbjct: 168 WNELGNGLAYDDFSFPIFLLEDENETKVIKQCYQDHNLGQNGSAPSFPLCAMQLFSHMHA 227

Query: 223 EFDLVMQTTKAGTRNSMSCLKEETCLPLGGYSVWSSLPPINISSDKSKPIILTVAS--MD 282
                    ++  +++ S   E  C PL  Y+VWS L PIN S      + + VA+  +D
Sbjct: 228 VISTATCMRRSFIQSTFSINPEIVCDPLSDYNVWSMLKPINTSGGLEPDVRVVVAATRLD 287

Query: 283 SASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGESWGYLGSRRF 342
           S SFF + + GA+S ++  +  LAA +AL     +  L + ++FV F GE++ Y+GS R 
Sbjct: 288 SRSFFWNVAPGAESAVASFVTQLAAAEALHKAPDVTTLPRNVMFVFFQGETFDYIGSSRM 347

Query: 343 LLELDLQSDSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSS-KNETWNALKL 402
           + +++     V  L N  ID+  E+G V  +++         M++ + S KN+  + L  
Sbjct: 348 VYDMENGKFPVR-LEN--IDSFVELGQVALRTSLELWMHTDPMSQKNESVKNQVEDLLVT 407

Query: 403 ARESLPFENIKVSPASTTNPGIPPSSLMAFLAKNSQVSGVVLEDFDTSFTNQFYQSHLDD 462
             +S       V      +  +PPSSL  FL +   +SGVVL D   SF N++YQS  D 
Sbjct: 408 LEQSGADTPQVVLSRLVQSQALPPSSLQRFL-RARNISGVVLADHSGSFHNRYYQSIYDT 467

Query: 463 LHNIN-------------------SSAIEAAALLVARTLYILATNKKELSSSALNAIKLN 522
             NIN                   + A+   A ++AR LY LA       ++  N+I+ +
Sbjct: 468 AENINVTYPESQSPEEDLNFVTDTAKALADVATVLARALYKLAG-----GTNFNNSIQAD 527

Query: 523 TSLVEELI-GCLLNCDPGLSCELVKRYI-SPTNVCP-NHYVGVILDEPSSTPYPGYVHDV 582
              V  L+ G L+  +      +++  + S  +  P  HY+ V    P++T Y      V
Sbjct: 528 PQTVTRLLYGFLVRANNSWFQSILRHDLRSYLDDGPLQHYIAV--SSPTNTTY------V 587

Query: 583 SRFAWNFLADRTSIRKENTSSVCSQNCDDKSEV------------CIGAETGKGT----- 642
            ++A   LA+ T       +++  + C D S+V              G      T     
Sbjct: 588 VQYA---LANLTG----KVTNLTQEQCQDPSKVPNESKDLYEYSWVQGPWNSNKTERLPR 647

Query: 643 CVVSTTRYVPAYSTRLTFDSGSWNVLPPNASDPMGAVDPVWTESNWNTIGLRMYTVQATA 647
           CV ST R   A S    F+   W+                W ES W  I  R++ + +  
Sbjct: 648 CVRSTVRLARALSP--AFELSQWS----------STEYSTWAESRWKDIQARIFLIASKE 682

BLAST of Carg07431 vs. ExPASy Swiss-Prot
Match: Q92542 (Nicastrin OS=Homo sapiens OX=9606 GN=NCSTN PE=1 SV=2)

HSP 1 Score: 136.7 bits (343), Expect = 9.4e-31
Identity = 159/666 (23.87%), Postives = 271/666 (40.69%), Query Frame = 0

Query: 43  PCIRLLNLSGEIGC--SNPGREKVVVPMINFKDADEILQPS------AILVSMDDISSFF 102
           PC+RLLN + +IGC  S  G   V+  +   +D   +L          +L S        
Sbjct: 49  PCVRLLNATHQIGCQSSISGDTGVIHVVEKEEDLQWVLTDGPNPPYMVLLESKHFTRDLM 108

Query: 103 ARLQDDSNFASNVGGVLIKPGTEIQKRTKGFSPAQKFPQAKFAPYQKI---------DYE 162
            +L+  ++  + +   L KP         GFSP+ + P   F  Y            + +
Sbjct: 109 EKLKGRTSRIAGLAVSLTKPSP-----ASGFSPSVQCPNDGFGVYSNSYGPEFAHCREIQ 168

Query: 163 WNPIGSGVMWNQYNFPVFLISES-------------SISPVQEAASKNVKDKKVYTSNVA 222
           WN +G+G+ +  ++FP+FL+ +              ++S    A +  +   ++++   A
Sbjct: 169 WNSLGNGLAYEDFSFPIFLLEDENETKVIKQCYQDHNLSQNGSAPTFPLCAMQLFSHMHA 228

Query: 223 EFDLVMQTTKAGTRNSMSCLKEETCLPLGGYSVWSSLPPINISSDKSKP---IILTVASM 282
                    ++  +++ S   E  C PL  Y+VWS L PIN ++   KP   +++    +
Sbjct: 229 VISTATCMRRSSIQSTFSINPEIVCDPLSDYNVWSMLKPIN-TTGTLKPDDRVVVAATRL 288

Query: 283 DSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGESWGYLGSRR 342
           DS SFF + + GA+S ++  +  LAA +AL     +  L + ++FV F GE++ Y+GS R
Sbjct: 289 DSRSFFWNVAPGAESAVASFVTQLAAAEALQKAPDVTTLPRNVMFVFFQGETFDYIGSSR 348

Query: 343 FLLELDLQSDSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSKNETWNALKL 402
            + +++     V  L N  +D+  E+G V  +++      + H   VS       N ++ 
Sbjct: 349 MVYDMEKGKFPVQ-LEN--VDSFVELGQVALRTS---LELWMHTDPVSQKNESVRNQVED 408

Query: 403 ARESLPFENIKVSPASTTNPG----IPPSSLMAFLAKNSQVSGVVLEDFDTSFTNQFYQS 462
              +L      V       P     +PPSSL  FL +   +SGVVL D   +F N++YQS
Sbjct: 409 LLATLEKSGAGVPAVILRRPNQSQPLPPSSLQRFL-RARNISGVVLADHSGAFHNKYYQS 468

Query: 463 HLDDLHNINSS-------------------AIEAAALLVARTLYILATNKKELSSSALNA 522
             D   NIN S                   A+   A ++ R LY LA       +   + 
Sbjct: 469 IYDTAENINVSYPEWLSPEEDLNFVTDTAKALADVATVLGRALYELAGGTNFSDTVQADP 528

Query: 523 IKLNTSLVEELIGCLLNCDPGLSCELVKRYISPTNVCPNHYVGVILDEPSSTPYPGYVHD 582
             +   L   LI    +    +  + ++ Y+    +   HY+ V    P++T Y   V  
Sbjct: 529 QTVTRLLYGFLIKANNSWFQSILRQDLRSYLGDGPL--QHYIAV--SSPTNTTY--VVQY 588

Query: 583 VSRFAWNFLADRTSIRKENTSSVCSQNCDDKSEVCI-----GAETGK-GTCVVSTTRYVP 642
                   + + T  + ++ S V S+N D      +       ET +   CV ST R   
Sbjct: 589 ALANLTGTVVNLTREQCQDPSKVPSENKDLYEYSWVQGPLHSNETDRLPRCVRSTARLAR 648

Query: 643 AYSTRLTFDSGSWNVLPPNASDPMGAVDPVWTESNWNTIGLRMYTVQATAYDRFVLLGGI 647
           A S    F+   W+                WTES W  I  R++ + +   +   L  G 
Sbjct: 649 ALSP--AFELSQWS----------STEYSTWTESRWKDIRARIFLIASKELELITLTVGF 683

BLAST of Carg07431 vs. ExPASy TrEMBL
Match: A0A6J1GAI9 (Nicastrin OS=Cucurbita moschata OX=3662 GN=LOC111452408 PE=3 SV=1)

HSP 1 Score: 1310.4 bits (3390), Expect = 0.0e+00
Identity = 660/664 (99.40%), Postives = 664/664 (100.00%), Query Frame = 0

Query: 1   MSFHILHLLLFLTSVRLCLSDEHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPG 60
           MSFHILHLLLFLTSVRLCLSDEHSMESVPDLQNSMYLVVDG+PCIRLLNLSGEIGCSNPG
Sbjct: 1   MSFHILHLLLFLTSVRLCLSDEHSMESVPDLQNSMYLVVDGHPCIRLLNLSGEIGCSNPG 60

Query: 61  REKVVVPMINFKDADEILQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQK 120
           REKVVVPMINFKDADEILQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQK
Sbjct: 61  REKVVVPMINFKDADEILQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQK 120

Query: 121 RTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKN 180
           RTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKN
Sbjct: 121 RTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKN 180

Query: 181 VKDKKVYTSNVAEFDLVMQTTKAGTRNSMSCLKEETCLPLGGYSVWSSLPPINISSDKSK 240
           VKDKK+YTSNVAEFDLVMQTTKAGTRNSMSCLKEETCLPLGGYSVWSSLPPINISSDKSK
Sbjct: 181 VKDKKIYTSNVAEFDLVMQTTKAGTRNSMSCLKEETCLPLGGYSVWSSLPPINISSDKSK 240

Query: 241 PIILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGE 300
           PIILTVASMDSASFFRDKSIGADSPISGLIALLA+VDALSHVDGLDDLHKQLVFVVFTGE
Sbjct: 241 PIILTVASMDSASFFRDKSIGADSPISGLIALLASVDALSHVDGLDDLHKQLVFVVFTGE 300

Query: 301 SWGYLGSRRFLLELDLQSDSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSK 360
           SWGYLGSRRFLLELDLQSDSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSK
Sbjct: 301 SWGYLGSRRFLLELDLQSDSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSK 360

Query: 361 NETWNALKLARESLPFENIKVSPASTTNPGIPPSSLMAFLAKNSQVSGVVLEDFDTSFTN 420
           NETWNALKLA+ESLPFENIKVSPASTTNPGIPPSSLMAFLAKNSQVSGVVLEDFDTSFTN
Sbjct: 361 NETWNALKLAQESLPFENIKVSPASTTNPGIPPSSLMAFLAKNSQVSGVVLEDFDTSFTN 420

Query: 421 QFYQSHLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEELIGC 480
           QFYQSHLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEELIGC
Sbjct: 421 QFYQSHLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEELIGC 480

Query: 481 LLNCDPGLSCELVKRYISPTNVCPNHYVGVILDEPSSTPYPGYVHDVSRFAWNFLADRTS 540
           LLNCDPGLSCELVKRYISPTNVCPNHYVGVILDEPSSTPYPGYVHDVSRFAWNFLADRTS
Sbjct: 481 LLNCDPGLSCELVKRYISPTNVCPNHYVGVILDEPSSTPYPGYVHDVSRFAWNFLADRTS 540

Query: 541 IRKENTSSVCSQNCDDKSEVCIGAETGKGTCVVSTTRYVPAYSTRLTFDSGSWNVLPPNA 600
           IRKENTSSVCSQNCDDKSEVCIGAETGKGTCVVSTTRYVPAYSTRLTFDSGSWNVLPPNA
Sbjct: 541 IRKENTSSVCSQNCDDKSEVCIGAETGKGTCVVSTTRYVPAYSTRLTFDSGSWNVLPPNA 600

Query: 601 SDPMGAVDPVWTESNWNTIGLRMYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKA 660
           SDPMGAVDPVWTESNWNTIGLRMYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKA
Sbjct: 601 SDPMGAVDPVWTESNWNTIGLRMYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKA 660

Query: 661 LKKD 665
           LKKD
Sbjct: 661 LKKD 664

BLAST of Carg07431 vs. ExPASy TrEMBL
Match: A0A6J1K5Z2 (Nicastrin OS=Cucurbita maxima OX=3661 GN=LOC111492619 PE=3 SV=1)

HSP 1 Score: 1290.8 bits (3339), Expect = 0.0e+00
Identity = 650/664 (97.89%), Postives = 655/664 (98.64%), Query Frame = 0

Query: 1   MSFHILHLLLFLTSVRLCLSDEHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPG 60
           M+FHILHLLLFLTS RLCLSDEHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPG
Sbjct: 1   MAFHILHLLLFLTSARLCLSDEHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPG 60

Query: 61  REKVVVPMINFKDADEILQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQK 120
           REKVVVPMINFKDADEI QPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQK
Sbjct: 61  REKVVVPMINFKDADEIFQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQK 120

Query: 121 RTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKN 180
           RTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKN
Sbjct: 121 RTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKN 180

Query: 181 VKDKKVYTSNVAEFDLVMQTTKAGTRNSMSCLKEETCLPLGGYSVWSSLPPINISSDKSK 240
           VKDKKVYTSNVAEFDLVMQTTKAGT NSMSCLKEETCLPLGGYSVWSSLPPINIS DKSK
Sbjct: 181 VKDKKVYTSNVAEFDLVMQTTKAGTHNSMSCLKEETCLPLGGYSVWSSLPPINISLDKSK 240

Query: 241 PIILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGE 300
           PIILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGE
Sbjct: 241 PIILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGE 300

Query: 301 SWGYLGSRRFLLELDLQSDSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSK 360
           SWGYLGSRRFLLELDLQSDSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSK
Sbjct: 301 SWGYLGSRRFLLELDLQSDSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSK 360

Query: 361 NETWNALKLARESLPFENIKVSPASTTNPGIPPSSLMAFLAKNSQVSGVVLEDFDTSFTN 420
           NETWNALKLA+ESLPFENIKVSPASTTNPGIPPSSLMAFLAKNSQVSGVVLEDFDTSFTN
Sbjct: 361 NETWNALKLAQESLPFENIKVSPASTTNPGIPPSSLMAFLAKNSQVSGVVLEDFDTSFTN 420

Query: 421 QFYQSHLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEELIGC 480
           QFYQSHLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEE+IGC
Sbjct: 421 QFYQSHLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEEIIGC 480

Query: 481 LLNCDPGLSCELVKRYISPTNVCPNHYVGVILDEPSSTPYPGYVHDVSRFAWNFLADRTS 540
           LLNCDPGLSCELVKRYISP NVCPNHYVGVILDEPSSTPYPGYVHDVSRF WNFLADRTS
Sbjct: 481 LLNCDPGLSCELVKRYISPINVCPNHYVGVILDEPSSTPYPGYVHDVSRFVWNFLADRTS 540

Query: 541 IRKENTSSVCSQNCDDKSEVCIGAETGKGTCVVSTTRYVPAYSTRLTFDSGSWNVLPPNA 600
           I KENTSSVCSQNCDDKSEVCIGAETGKGTCVVSTTRYVPAYSTRL F+SGSWNVLPPN+
Sbjct: 541 IPKENTSSVCSQNCDDKSEVCIGAETGKGTCVVSTTRYVPAYSTRLMFESGSWNVLPPNS 600

Query: 601 SDPMGAVDPVWTESNWNTIGLRMYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKA 660
           SDPMGAVDPVWTESNWNTIGLR YTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKA
Sbjct: 601 SDPMGAVDPVWTESNWNTIGLRTYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKA 660

Query: 661 LKKD 665
           LKKD
Sbjct: 661 LKKD 664

BLAST of Carg07431 vs. ExPASy TrEMBL
Match: A0A6J1CNM4 (Nicastrin OS=Momordica charantia OX=3673 GN=LOC111013258 PE=3 SV=1)

HSP 1 Score: 1183.3 bits (3060), Expect = 0.0e+00
Identity = 590/665 (88.72%), Postives = 632/665 (95.04%), Query Frame = 0

Query: 1   MSFHILHLLLFLTSVRLCLSDE-HSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNP 60
           MS +IL+LL+FLTS+RL LSD+ HSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNP
Sbjct: 1   MSSNILYLLVFLTSLRLSLSDDTHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNP 60

Query: 61  GREKVVVPMINFKDADEILQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQ 120
           GREKVVVPMINFKDADE+ QPSAI+VSMD+ISSFF+RL+DDSNFA+NVGGVLI+PGTE+Q
Sbjct: 61  GREKVVVPMINFKDADELSQPSAIVVSMDEISSFFSRLKDDSNFANNVGGVLIEPGTEMQ 120

Query: 121 KRTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASK 180
            RT+GFSPA+KFPQA+FAPYQK+DY+WNPIGSG+MW QYNFPVFLISE+SIS + EA+SK
Sbjct: 121 NRTEGFSPARKFPQAEFAPYQKVDYDWNPIGSGIMWKQYNFPVFLISETSISSIHEASSK 180

Query: 181 NVKDKKVYTSNVAEFDLVMQTTKAGTRNSMSCLKEETCLPLGGYSVWSSLPPINISSDKS 240
           NVK+KK YTSNVAEFDLVMQTTKAGT NS+SCLKEETCLPLGGYSVWSSLPPINISSD+S
Sbjct: 181 NVKNKKAYTSNVAEFDLVMQTTKAGTHNSVSCLKEETCLPLGGYSVWSSLPPINISSDQS 240

Query: 241 KPIILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTG 300
           KPIILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVF VFTG
Sbjct: 241 KPIILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFAVFTG 300

Query: 301 ESWGYLGSRRFLLELDLQSDSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSS 360
           ESWGYLGSRRFLLELDLQSD VSGLNN LIDTVFEIGSVGK S+ G GNFFAHMTEVSSS
Sbjct: 301 ESWGYLGSRRFLLELDLQSDVVSGLNNKLIDTVFEIGSVGKSSSHGIGNFFAHMTEVSSS 360

Query: 361 KNETWNALKLARESLPFENIKVSPASTTNPGIPPSSLMAFLAKNSQVSGVVLEDFDTSFT 420
           KNETWNALK A+ESLPFE  K+SPASTTNPGIPPSSLMAFL KNS VSGVVLEDFDT FT
Sbjct: 361 KNETWNALKHAQESLPFEITKISPASTTNPGIPPSSLMAFLKKNSHVSGVVLEDFDTGFT 420

Query: 421 NQFYQSHLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEELIG 480
           NQFYQSHLDDL+NINSSAIEAAALLVARTLYILATNKKEL SSA+ +IK+NTSLVEELIG
Sbjct: 421 NQFYQSHLDDLYNINSSAIEAAALLVARTLYILATNKKELISSAITSIKVNTSLVEELIG 480

Query: 481 CLLNCDPGLSCELVKRYISPTNVCPNHYVGVILDEPSSTPYPGYVHDVSRFAWNFLADRT 540
           CLLNCDPGLSCELVKRYISPT+VCPNHYVGVILDEPSSTPYPGYVHDVSRF WNFLADRT
Sbjct: 481 CLLNCDPGLSCELVKRYISPTSVCPNHYVGVILDEPSSTPYPGYVHDVSRFVWNFLADRT 540

Query: 541 SIRKENTSSVCSQNCDDKSEVCIGAETGKGTCVVSTTRYVPAYSTRLTFDSGSWNVLPPN 600
           SI KENTSS CS NC+DKSEVCIGAE GKGTCV+STTRYVPAYSTRL ++SGSW VLP N
Sbjct: 541 SISKENTSSACSPNCNDKSEVCIGAEIGKGTCVISTTRYVPAYSTRLKYESGSWYVLPSN 600

Query: 601 ASDPMGAVDPVWTESNWNTIGLRMYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMK 660
           +SDPMGAVDPVWTESNWNTIGLRMYT+Q TAYDRFVLLGGITTTIL+YFAIVAVRGSI+K
Sbjct: 601 SSDPMGAVDPVWTESNWNTIGLRMYTIQTTAYDRFVLLGGITTTILAYFAIVAVRGSIIK 660

Query: 661 ALKKD 665
           ALK+D
Sbjct: 661 ALKRD 665

BLAST of Carg07431 vs. ExPASy TrEMBL
Match: A0A1S3C4C7 (Nicastrin OS=Cucumis melo OX=3656 GN=LOC103496866 PE=3 SV=1)

HSP 1 Score: 1173.7 bits (3035), Expect = 0.0e+00
Identity = 588/665 (88.42%), Postives = 624/665 (93.83%), Query Frame = 0

Query: 1   MSFHILHLLLFLTSVRLCLSDEHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPG 60
           MS   L+LLLFLTS+RL  SDE  MESVPDLQNSMYL VDGYPCIRLLNLSGEIGCSNPG
Sbjct: 19  MSSQFLYLLLFLTSLRLSSSDEQKMESVPDLQNSMYLAVDGYPCIRLLNLSGEIGCSNPG 78

Query: 61  REKVVVPMINFKDADEILQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQK 120
           REKVV+PMINFKDADEIL+PSA+LVSMD ISSFF RLQDDS+FA+NVGGVLI+PGT IQ 
Sbjct: 79  REKVVLPMINFKDADEILEPSAVLVSMDAISSFFTRLQDDSHFANNVGGVLIEPGTGIQN 138

Query: 121 RTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKN 180
           RT+GFSPAQKFPQAKFAPY+K DYEWNPIGSG+MWN+YNFPVFLISESSIS +QEAASKN
Sbjct: 139 RTEGFSPAQKFPQAKFAPYKKNDYEWNPIGSGIMWNRYNFPVFLISESSISSIQEAASKN 198

Query: 181 VKDKKVYTSNVAEFDLVMQTTKAGTRNSMSCLKEETCLPLGGYSVWSSLPPINI-SSDKS 240
           VK KK Y SNVAEFDLVMQTTKAGT +SMSCLKEETCLPLGGYSVWSSLPPIN  SSD+S
Sbjct: 199 VKSKKDYVSNVAEFDLVMQTTKAGTHSSMSCLKEETCLPLGGYSVWSSLPPINTSSSDQS 258

Query: 241 KPIILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTG 300
           KP+ILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTG
Sbjct: 259 KPVILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTG 318

Query: 301 ESWGYLGSRRFLLELDLQSDSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSS 360
           ESWGYLGSRRFLLELDLQSD+VSGL+N LID VFEIGSVGK SN G GNFFAHMTEVSSS
Sbjct: 319 ESWGYLGSRRFLLELDLQSDAVSGLSNRLIDMVFEIGSVGKSSNHGSGNFFAHMTEVSSS 378

Query: 361 KNETWNALKLARESLPFENIKVSPASTTNPGIPPSSLMAFLAKNSQVSGVVLEDFDTSFT 420
           KNETWNALKLARESLP ENIKVSPASTTNPGIPPSSLMAFLAKN Q+SGVVL+DFDT FT
Sbjct: 379 KNETWNALKLARESLPLENIKVSPASTTNPGIPPSSLMAFLAKNPQISGVVLDDFDTGFT 438

Query: 421 NQFYQSHLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEELIG 480
           NQFYQSHLDDLHNINSSAIEAAALLVARTLYILA NK ELSSS L AIK+NTSLVEELIG
Sbjct: 439 NQFYQSHLDDLHNINSSAIEAAALLVARTLYILAINKNELSSSVLTAIKVNTSLVEELIG 498

Query: 481 CLLNCDPGLSCELVKRYISPTNVCPNHYVGVILDEPSSTPYPGYVHDVSRFAWNFLADRT 540
           CLLNCDPGLSCELVKRYI+P++VCPNHYVGVILDEPSS PYP YVHDVSRF WNFLADRT
Sbjct: 499 CLLNCDPGLSCELVKRYITPSSVCPNHYVGVILDEPSSAPYPDYVHDVSRFVWNFLADRT 558

Query: 541 SIRKENTSSVCSQNCDDKSEVCIGAETGKGTCVVSTTRYVPAYSTRLTFDSGSWNVLPPN 600
           SI KENTSSVCSQNCDD+SEVCIGAETGKGTCV+STTRY+PAYSTRL F+SG W+VLPPN
Sbjct: 559 SIPKENTSSVCSQNCDDRSEVCIGAETGKGTCVISTTRYIPAYSTRLKFESGYWSVLPPN 618

Query: 601 ASDPMGAVDPVWTESNWNTIGLRMYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMK 660
           +SD +GAVDPVWTESNWNTIGLR+YT+QA AYDRFVLLGGITTTIL+YFAIVAVR SI+K
Sbjct: 619 SSDHLGAVDPVWTESNWNTIGLRIYTIQAAAYDRFVLLGGITTTILAYFAIVAVRSSIIK 678

Query: 661 ALKKD 665
           ALK+D
Sbjct: 679 ALKRD 683

BLAST of Carg07431 vs. ExPASy TrEMBL
Match: A0A1S3C4S1 (Nicastrin OS=Cucumis melo OX=3656 GN=LOC103496866 PE=3 SV=1)

HSP 1 Score: 1119.0 bits (2893), Expect = 0.0e+00
Identity = 567/665 (85.26%), Postives = 603/665 (90.68%), Query Frame = 0

Query: 1   MSFHILHLLLFLTSVRLCLSDEHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPG 60
           MS   L+LLLFLTS+RL  SDE  MESVPDLQNSMYL VDGYPCIRLLNLSGEIGCSNPG
Sbjct: 19  MSSQFLYLLLFLTSLRLSSSDEQKMESVPDLQNSMYLAVDGYPCIRLLNLSGEIGCSNPG 78

Query: 61  REKVVVPMINFKDADEILQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQK 120
           REKVV+PMINFKDADEIL+PSA+LVSMD ISSFF RLQDDS+FA+NVGGVLI+PGT IQ 
Sbjct: 79  REKVVLPMINFKDADEILEPSAVLVSMDAISSFFTRLQDDSHFANNVGGVLIEPGTGIQN 138

Query: 121 RTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKN 180
           RT+GFSPAQKFPQAKFAPY+K DYEWNPIGSG+MWN+YNFPVFLISESSIS +QEAASKN
Sbjct: 139 RTEGFSPAQKFPQAKFAPYKKNDYEWNPIGSGIMWNRYNFPVFLISESSISSIQEAASKN 198

Query: 181 VKDKKVYTSNVAEFDLVMQTTKAGTRNSMSCLKEETCLPLGGYSVWSSLPPINI-SSDKS 240
           VK KK Y SNVAEFDLVMQTTKAGT +SMSCLKEETCLPLGGYSVWSSLPPIN  SSD+S
Sbjct: 199 VKSKKDYVSNVAEFDLVMQTTKAGTHSSMSCLKEETCLPLGGYSVWSSLPPINTSSSDQS 258

Query: 241 KPIILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTG 300
           KP+ILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTG
Sbjct: 259 KPVILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTG 318

Query: 301 ESWGYLGSRRFLLELDLQSDSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSS 360
           ESWGYLGSRRFLLELDLQSD+VSGL+N LID                         VSSS
Sbjct: 319 ESWGYLGSRRFLLELDLQSDAVSGLSNRLIDM------------------------VSSS 378

Query: 361 KNETWNALKLARESLPFENIKVSPASTTNPGIPPSSLMAFLAKNSQVSGVVLEDFDTSFT 420
           KNETWNALKLARESLP ENIKVSPASTTNPGIPPSSLMAFLAKN Q+SGVVL+DFDT FT
Sbjct: 379 KNETWNALKLARESLPLENIKVSPASTTNPGIPPSSLMAFLAKNPQISGVVLDDFDTGFT 438

Query: 421 NQFYQSHLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEELIG 480
           NQFYQSHLDDLHNINSSAIEAAALLVARTLYILA NK ELSSS L AIK+NTSLVEELIG
Sbjct: 439 NQFYQSHLDDLHNINSSAIEAAALLVARTLYILAINKNELSSSVLTAIKVNTSLVEELIG 498

Query: 481 CLLNCDPGLSCELVKRYISPTNVCPNHYVGVILDEPSSTPYPGYVHDVSRFAWNFLADRT 540
           CLLNCDPGLSCELVKRYI+P++VCPNHYVGVILDEPSS PYP YVHDVSRF WNFLADRT
Sbjct: 499 CLLNCDPGLSCELVKRYITPSSVCPNHYVGVILDEPSSAPYPDYVHDVSRFVWNFLADRT 558

Query: 541 SIRKENTSSVCSQNCDDKSEVCIGAETGKGTCVVSTTRYVPAYSTRLTFDSGSWNVLPPN 600
           SI KENTSSVCSQNCDD+SEVCIGAETGKGTCV+STTRY+PAYSTRL F+SG W+VLPPN
Sbjct: 559 SIPKENTSSVCSQNCDDRSEVCIGAETGKGTCVISTTRYIPAYSTRLKFESGYWSVLPPN 618

Query: 601 ASDPMGAVDPVWTESNWNTIGLRMYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMK 660
           +SD +GAVDPVWTESNWNTIGLR+YT+QA AYDRFVLLGGITTTIL+YFAIVAVR SI+K
Sbjct: 619 SSDHLGAVDPVWTESNWNTIGLRIYTIQAAAYDRFVLLGGITTTILAYFAIVAVRSSIIK 659

Query: 661 ALKKD 665
           ALK+D
Sbjct: 679 ALKRD 659

BLAST of Carg07431 vs. TAIR 10
Match: AT3G52640.1 (Zn-dependent exopeptidases superfamily protein )

HSP 1 Score: 844.0 bits (2179), Expect = 8.4e-245
Identity = 421/661 (63.69%), Postives = 519/661 (78.52%), Query Frame = 0

Query: 8   LLLFLTSVRLCLSDE-HSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPGREKVVV 67
           +LL +  + L L+DE  S+ESVPDLQ  MY+ VDG+PC+RLLNLSGEIGCSNPG  KVV 
Sbjct: 16  VLLSILPLHLSLADEITSIESVPDLQKLMYVAVDGFPCVRLLNLSGEIGCSNPGINKVVA 75

Query: 68  PMINFKDADEILQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQKRTKGFS 127
           P+I  KD  +++QP  ILV+ D++  FF R+  D +FAS +GGVL++ G+  Q++ KGFS
Sbjct: 76  PIIKLKDVKDLVQPHTILVTADEMEDFFTRVSTDLSFASKIGGVLVESGSNFQQKLKGFS 135

Query: 128 PAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKNVKDKKV 187
           P ++FPQA+F+PY+ ++Y+WN   S +MW  YNFPV+L+SES IS V E  SK       
Sbjct: 136 PDKRFPQAQFSPYENVEYKWNSAASSIMWRNYNFPVYLLSESGISAVHEILSKKKMKHGT 195

Query: 188 YTSNVAEFDLVMQTTKAGTRNSMSCLKEETCLPLGGYSVWSSLPPINISSDKS-KPIILT 247
           YTS+VAEF++VM+TTKAGT NS +CL+E TCLPLGGYSVWSSLPPI++SS  + KP++LT
Sbjct: 196 YTSDVAEFNMVMETTKAGTHNSEACLQEGTCLPLGGYSVWSSLPPISVSSSNNRKPVVLT 255

Query: 248 VASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGESWGYL 307
           VASMD+ASFFRDKS GADSPISGL+ALL AVDALS VDG+ +L KQLVF+V TGE+WGYL
Sbjct: 256 VASMDTASFFRDKSFGADSPISGLVALLGAVDALSRVDGISNLKKQLVFLVLTGETWGYL 315

Query: 308 GSRRFLLELDLQSDSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSKNETWN 367
           GSRRFL ELDL SD+V+GL+NT I+TV EIGSVGK  + G   FFAH T VSS  N T +
Sbjct: 316 GSRRFLHELDLHSDAVAGLSNTSIETVLEIGSVGKGLSGGINTFFAHKTRVSSVTNMTLD 375

Query: 368 ALKLARESLPFENIKVSPASTTNPGIPPSSLMAFLAKNSQVSGVVLEDFDTSFTNQFYQS 427
           ALK+A++SL  +NIK+  A T NPGIPPSSLMAF+ KN Q S VVLEDFDT+F N+FY S
Sbjct: 376 ALKIAQDSLASKNIKILSADTANPGIPPSSLMAFMRKNPQTSAVVLEDFDTNFVNKFYHS 435

Query: 428 HLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEELIGCLLNCD 487
           HLDDL NINSS++ AAA +VARTLYILA++ K+ S+SAL +I +N S VEEL+ CLL C+
Sbjct: 436 HLDDLSNINSSSVVAAASVVARTLYILASDNKDTSNSALGSIHVNASFVEELLTCLLACE 495

Query: 488 PGLSCELVKRYISPTNVCPNHYVGVILDEPSSTPYPGYVHDVSRFAWNFLADRTSIRKEN 547
           PGLSC LVK YISPTN CP +Y GVIL EPSS PY GYV DVSRF WNFLAD+TS++K N
Sbjct: 496 PGLSCNLVKDYISPTNTCPGNYAGVILGEPSSKPYLGYVGDVSRFLWNFLADKTSVQKGN 555

Query: 548 TSSVCSQN-CDDKSEVCIGAETGK-GTCVVSTTRYVPAYSTRLTFDSGSWNVLPPNASDP 607
           T+SVCS+  C    EVCI AE+ K GTCVVSTTRYVPAYSTRL ++ G+W +LP N+SD 
Sbjct: 556 TTSVCSKGVCSKTDEVCIKAESNKEGTCVVSTTRYVPAYSTRLKYNDGAWTILPQNSSDS 615

Query: 608 MGAVDPVWTESNWNTIGLRMYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKALKK 665
           MG VDPVWTESNW+T+ + +YTVQ +AYD  VL+ GIT T L+Y  I+A +  I KALK+
Sbjct: 616 MGMVDPVWTESNWDTLRVHVYTVQHSAYDNAVLVAGITVTTLAYIGILAAKSIITKALKQ 675

BLAST of Carg07431 vs. TAIR 10
Match: AT3G52640.2 (Zn-dependent exopeptidases superfamily protein )

HSP 1 Score: 828.2 bits (2138), Expect = 4.8e-240
Identity = 421/690 (61.01%), Postives = 519/690 (75.22%), Query Frame = 0

Query: 8   LLLFLTSVRLCLSDE-HSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPGREKVVV 67
           +LL +  + L L+DE  S+ESVPDLQ  MY+ VDG+PC+RLLNLSGEIGCSNPG  KVV 
Sbjct: 16  VLLSILPLHLSLADEITSIESVPDLQKLMYVAVDGFPCVRLLNLSGEIGCSNPGINKVVA 75

Query: 68  PMINFKDADEILQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQKRTKGFS 127
           P+I  KD  +++QP  ILV+ D++  FF R+  D +FAS +GGVL++ G+  Q++ KGFS
Sbjct: 76  PIIKLKDVKDLVQPHTILVTADEMEDFFTRVSTDLSFASKIGGVLVESGSNFQQKLKGFS 135

Query: 128 PAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKNVKDKKV 187
           P ++FPQA+F+PY+ ++Y+WN   S +MW  YNFPV+L+SES IS V E  SK       
Sbjct: 136 PDKRFPQAQFSPYENVEYKWNSAASSIMWRNYNFPVYLLSESGISAVHEILSKKKMKHGT 195

Query: 188 YTSNVAEFDLVMQTTKAGTRNSMSCLKEETCLPLGGYSVWSSLPPINISSDKS-KPIILT 247
           YTS+VAEF++VM+TTKAGT NS +CL+E TCLPLGGYSVWSSLPPI++SS  + KP++LT
Sbjct: 196 YTSDVAEFNMVMETTKAGTHNSEACLQEGTCLPLGGYSVWSSLPPISVSSSNNRKPVVLT 255

Query: 248 VASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGESWGYL 307
           VASMD+ASFFRDKS GADSPISGL+ALL AVDALS VDG+ +L KQLVF+V TGE+WGYL
Sbjct: 256 VASMDTASFFRDKSFGADSPISGLVALLGAVDALSRVDGISNLKKQLVFLVLTGETWGYL 315

Query: 308 GSRRFLLELDLQSDSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSKNETWN 367
           GSRRFL ELDL SD+V+GL+NT I+TV EIGSVGK  + G   FFAH T VSS  N T +
Sbjct: 316 GSRRFLHELDLHSDAVAGLSNTSIETVLEIGSVGKGLSGGINTFFAHKTRVSSVTNMTLD 375

Query: 368 ALKLARESLPFENIKVSPASTTNPGIPPSSLMAFLAKNSQVSGVVLEDFDTSFTNQFYQS 427
           ALK+A++SL  +NIK+  A T NPGIPPSSLMAF+ KN Q S VVLEDFDT+F N+FY S
Sbjct: 376 ALKIAQDSLASKNIKILSADTANPGIPPSSLMAFMRKNPQTSAVVLEDFDTNFVNKFYHS 435

Query: 428 HLDDL-----------------------------HNINSSAIEAAALLVARTLYILATNK 487
           HLDDL                              NINSS++ AAA +VARTLYILA++ 
Sbjct: 436 HLDDLCKKSHSLSFSSFRSKPHFALLIPFWCCIAANINSSSVVAAASVVARTLYILASDN 495

Query: 488 KELSSSALNAIKLNTSLVEELIGCLLNCDPGLSCELVKRYISPTNVCPNHYVGVILDEPS 547
           K+ S+SAL +I +N S VEEL+ CLL C+PGLSC LVK YISPTN CP +Y GVIL EPS
Sbjct: 496 KDTSNSALGSIHVNASFVEELLTCLLACEPGLSCNLVKDYISPTNTCPGNYAGVILGEPS 555

Query: 548 STPYPGYVHDVSRFAWNFLADRTSIRKENTSSVCSQN-CDDKSEVCIGAETGK-GTCVVS 607
           S PY GYV DVSRF WNFLAD+TS++K NT+SVCS+  C    EVCI AE+ K GTCVVS
Sbjct: 556 SKPYLGYVGDVSRFLWNFLADKTSVQKGNTTSVCSKGVCSKTDEVCIKAESNKEGTCVVS 615

Query: 608 TTRYVPAYSTRLTFDSGSWNVLPPNASDPMGAVDPVWTESNWNTIGLRMYTVQATAYDRF 665
           TTRYVPAYSTRL ++ G+W +LP N+SD MG VDPVWTESNW+T+ + +YTVQ +AYD  
Sbjct: 616 TTRYVPAYSTRLKYNDGAWTILPQNSSDSMGMVDPVWTESNWDTLRVHVYTVQHSAYDNA 675

BLAST of Carg07431 vs. TAIR 10
Match: AT3G52640.3 (Zn-dependent exopeptidases superfamily protein )

HSP 1 Score: 716.8 bits (1849), Expect = 1.6e-206
Identity = 369/603 (61.19%), Postives = 452/603 (74.96%), Query Frame = 0

Query: 8   LLLFLTSVRLCLSDE-HSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPGREKVVV 67
           +LL +  + L L+DE  S+ESVPDLQ  MY+ VDG+PC+RLLNLSGEIGCSNPG  KVV 
Sbjct: 16  VLLSILPLHLSLADEITSIESVPDLQKLMYVAVDGFPCVRLLNLSGEIGCSNPGINKVVA 75

Query: 68  PMINFKDADEILQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQKRTKGFS 127
           P+I  KD  +++QP  ILV+ D++  FF R+  D +FAS +GGVL++ G+  Q++ KGFS
Sbjct: 76  PIIKLKDVKDLVQPHTILVTADEMEDFFTRVSTDLSFASKIGGVLVESGSNFQQKLKGFS 135

Query: 128 PAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKNVKDKKV 187
           P ++FPQA+F+PY+ ++Y+WN   S +MW  YNFPV+L+SES IS V E  SK       
Sbjct: 136 PDKRFPQAQFSPYENVEYKWNSAASSIMWRNYNFPVYLLSESGISAVHEILSKKKMKHGT 195

Query: 188 YTSNVAEFDLVMQTTKAGTRNSMSCLKEETCLPLGGYSVWSSLPPINISSDKS-KPIILT 247
           YTS+VAEF++VM+TTKAGT NS +CL+E TCLPLGGYSVWSSLPPI++SS  + KP++LT
Sbjct: 196 YTSDVAEFNMVMETTKAGTHNSEACLQEGTCLPLGGYSVWSSLPPISVSSSNNRKPVVLT 255

Query: 248 VASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGESWGYL 307
           VASMD+ASFFRDKS GADSPISGL+ALL AVDALS VDG+ +L KQLVF+V TGE+WGYL
Sbjct: 256 VASMDTASFFRDKSFGADSPISGLVALLGAVDALSRVDGISNLKKQLVFLVLTGETWGYL 315

Query: 308 GSRRFLLELDLQSDSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSKNETWN 367
           GSRRFL ELDL SD+V+GL+NT I+TV EIGSVGK  + G   FFAH T VSS  N T +
Sbjct: 316 GSRRFLHELDLHSDAVAGLSNTSIETVLEIGSVGKGLSGGINTFFAHKTRVSSVTNMTLD 375

Query: 368 ALKLARESLPFENIKVSPASTTNPGIPPSSLMAFLAKNSQVSGVVLEDFDTSFTNQFYQS 427
           ALK+A++SL  +NIK+  A T NPGIPPSSLMAF+ KN Q S VVLEDFDT+F N+FY S
Sbjct: 376 ALKIAQDSLASKNIKILSADTANPGIPPSSLMAFMRKNPQTSAVVLEDFDTNFVNKFYHS 435

Query: 428 HLDDL-----------------------------HNINSSAIEAAALLVARTLYILATNK 487
           HLDDL                              NINSS++ AAA +VARTLYILA++ 
Sbjct: 436 HLDDLCKKSHSLSFSSFRSKPHFALLIPFWCCIAANINSSSVVAAASVVARTLYILASDN 495

Query: 488 KELSSSALNAIKLNTSLVEELIGCLLNCDPGLSCELVKRYISPTNVCPNHYVGVILDEPS 547
           K+ S+SAL +I +N S VEEL+ CLL C+PGLSC LVK YISPTN CP +Y GVIL EPS
Sbjct: 496 KDTSNSALGSIHVNASFVEELLTCLLACEPGLSCNLVKDYISPTNTCPGNYAGVILGEPS 555

Query: 548 STPYPGYVHDVSRFAWNFLADRTSIRKENTSSVCSQN-CDDKSEVCIGAETGK-GTCVVS 578
           S PY GYV DVSRF WNFLAD+TS++K NT+SVCS+  C    EVCI AE+ K GTCVVS
Sbjct: 556 SKPYLGYVGDVSRFLWNFLADKTSVQKGNTTSVCSKGVCSKTDEVCIKAESNKEGTCVVS 615

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7036691.10.0e+00100.00Nicastrin [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022948878.10.0e+0099.40nicastrin [Cucurbita moschata][more]
KAG6606989.10.0e+0099.55Nicastrin, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023525268.10.0e+0099.10nicastrin [Cucurbita pepo subsp. pepo][more]
XP_022997757.10.0e+0097.89nicastrin [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q8GUM51.2e-24363.69Nicastrin OS=Arabidopsis thaliana OX=3702 GN=At3g52640/At3g52650 PE=2 SV=1[more]
F0ZBA62.6e-4925.70Nicastrin OS=Dictyostelium purpureum OX=5786 GN=ncstn PE=1 SV=1[more]
Q54JT71.3e-4826.35Nicastrin OS=Dictyostelium discoideum OX=44689 GN=ncstn PE=3 SV=2[more]
Q8CGU67.2e-3124.56Nicastrin OS=Rattus norvegicus OX=10116 GN=Ncstn PE=1 SV=1[more]
Q925429.4e-3123.87Nicastrin OS=Homo sapiens OX=9606 GN=NCSTN PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1GAI90.0e+0099.40Nicastrin OS=Cucurbita moschata OX=3662 GN=LOC111452408 PE=3 SV=1[more]
A0A6J1K5Z20.0e+0097.89Nicastrin OS=Cucurbita maxima OX=3661 GN=LOC111492619 PE=3 SV=1[more]
A0A6J1CNM40.0e+0088.72Nicastrin OS=Momordica charantia OX=3673 GN=LOC111013258 PE=3 SV=1[more]
A0A1S3C4C70.0e+0088.42Nicastrin OS=Cucumis melo OX=3656 GN=LOC103496866 PE=3 SV=1[more]
A0A1S3C4S10.0e+0085.26Nicastrin OS=Cucumis melo OX=3656 GN=LOC103496866 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G52640.18.4e-24563.69Zn-dependent exopeptidases superfamily protein [more]
AT3G52640.24.8e-24061.01Zn-dependent exopeptidases superfamily protein [more]
AT3G52640.31.6e-20661.19Zn-dependent exopeptidases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF05450Nicastrincoord: 241..439
e-value: 1.4E-65
score: 221.0
NoneNo IPR availableGENE3D3.40.630.10Zn peptidasescoord: 183..468
e-value: 7.1E-20
score: 73.6
NoneNo IPR availableCDDcd03881M28_Nicastrincoord: 39..617
e-value: 1.43249E-145
score: 431.46
NoneNo IPR availableSUPERFAMILY53187Zn-dependent exopeptidasescoord: 216..456
IPR041084Nicastrin, small lobePFAMPF18266Ncstrn_smallcoord: 43..200
e-value: 3.0E-39
score: 134.8
IPR008710NicastrinPANTHERPTHR21092NICASTRINcoord: 17..663

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg07431-RACarg07431-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016485 protein processing
cellular_component GO:0005887 integral component of plasma membrane
cellular_component GO:0016021 integral component of membrane