Carg07428 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg07428
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Kinesin light chain 3
Location	Carg_Chr01: 1743031 .. 1744489 (-)
RNA-Seq Expression	Carg07428
Synteny	Carg07428

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACACCCATTAGCCTCTGTGGTGCAGCTATAATTCTTGGAATAAACTCAAATCCTGTCTTAGCTAAAGACGCACCCTCTAAGCCAAGTTCTGAAAATGGTATTGAGGAGAGTGACACTATTGGACTACGCAAAGTGGAGGATGGTTCTGTAATTTCAAATCTACATACTTCAAAGTGGAGGGTTTTTACTGATAATGCTCGGGACTTGTTTCTGCAGGCAAGCATGTTTCTTTTACCTTCACTAGCAGTTAATTTTTTTAATGATTTGCAGAAAGAAGTATCATAATCTTTTTCTTTAATTAGGGGAAACTTGAAGAAGCTGAAAGATTTTTTGTTTCTGCGATTCAAGAAGCTAAAGAAGGTTTTGGGGAGAGGGATCCTCATGTTGCTTCTGCCTTCAACAATCTGGTATTGTAGCTGCTGTATTTTCTAATCTTTTTCTTGTTTTACCTTTTTAGAATCATTGAGTAGAACTTTCCTGCAGTCACTGAATAACCTAATGGAATAAGATTTCTTCCAACCAAGATATTAGACAAAATATTTGGAGTAGTAAAATAACCTGCCTTTAGGCTGTTGCCCTCCAAGATTGGGAGGCTGTGCTTTATTAGATTGGTTGAGTAGAGGGAACCCTCACCCCTCTACCCTTAGGCTGTTCTCATTTTGTCAGTATATTTCTGTTTTAAATATTTTTTTATATTAAAGAAGCCCAGTCTTTAGATGAATCATATTATTTTGGTTATTAGTTATTATTAAACCTGGAGACTTTTTTGCTTCATCAAAGTCATCATTTAGTTTATATATATATATCAAGATACAGACTGATCGTTAATCAAAGAACAAACAAGAAAGTAAGGATGTAAATTCCAAAATGAGGTCTTCATGTTGTTGGAAATAGTATCTAAATTTGTTGGGCTAGATTACCTACAAGTTTTTGCCATATTTGCATTTGTGCGTTCCTATTATTTACATTAAATTGACGTAGAACCTAGGTTGTTAGAATTCAGGAATGGTTCTAATATCAAAACTTCTTTACTAAAATCAGGCATTTACCTTGCATAGAAAAATCTACAATCAAGGACTGTTATACGATAATGCCATTTATTTTAGATTGTGAAGCATTACTAAGTAATAAATACATTTTGCTATGCTCTTTTTAGGCAGAACTGTATAGAATGGTTAGTGACCACTGAAGGATGGCACTCATCATTCCCTTGACAGAAAGGCAGAGGCCTGCCCAGTATCTGGAGGCCGTTGAGTCGATAGACTGTGGATCCCGAGTGGTCAGGGGGACTGGAGGGGGAATGCCGGCTTATGGCTCTGAGACAAGTCTGAGTAGCCGAGCGAGGGCGTTCTTGGAACTCTATGCTATCGCACTTTTTATCTCATATGTAGTCACAACCCTGGGCACAAAAACATGGGTAGTTTACATCATTAACTACACCCTCGACATGAACTAG

mRNA sequence

ATGACACCCATTAGCCTCTGTGGTGCAGCTATAATTCTTGGAATAAACTCAAATCCTGTCTTAGCTAAAGACGCACCCTCTAAGCCAAGTTCTGAAAATGGTATTGAGGAGAGTGACACTATTGGACTACGCAAAGTGGAGGATGGTTCTGGGAAACTTGAAGAAGCTGAAAGATTTTTTGTTTCTGCGATTCAAGAAGCTAAAGAAGGTTTTGGGGAGAGGGATCCTCATGTTGCTTCTGCCTTCAACAATCTGAGGCCTGCCCAGTATCTGGAGGCCGTTGAGTCGATAGACTGTGGATCCCGAGTGGTCAGGGGGACTGGAGGGGGAATGCCGGCTTATGGCTCTGAGACAAGTCTGAGTAGCCGAGCGAGGGCGTTCTTGGAACTCTATGCTATCGCACTTTTTATCTCATATGTAGTCACAACCCTGGGCACAAAAACATGGGTAGTTTACATCATTAACTACACCCTCGACATGAACTAG

Coding sequence (CDS)

ATGACACCCATTAGCCTCTGTGGTGCAGCTATAATTCTTGGAATAAACTCAAATCCTGTCTTAGCTAAAGACGCACCCTCTAAGCCAAGTTCTGAAAATGGTATTGAGGAGAGTGACACTATTGGACTACGCAAAGTGGAGGATGGTTCTGGGAAACTTGAAGAAGCTGAAAGATTTTTTGTTTCTGCGATTCAAGAAGCTAAAGAAGGTTTTGGGGAGAGGGATCCTCATGTTGCTTCTGCCTTCAACAATCTGAGGCCTGCCCAGTATCTGGAGGCCGTTGAGTCGATAGACTGTGGATCCCGAGTGGTCAGGGGGACTGGAGGGGGAATGCCGGCTTATGGCTCTGAGACAAGTCTGAGTAGCCGAGCGAGGGCGTTCTTGGAACTCTATGCTATCGCACTTTTTATCTCATATGTAGTCACAACCCTGGGCACAAAAACATGGGTAGTTTACATCATTAACTACACCCTCGACATGAACTAG

Protein sequence

MTPISLCGAAIILGINSNPVLAKDAPSKPSSENGIEESDTIGLRKVEDGSGKLEEAERFFVSAIQEAKEGFGERDPHVASAFNNLRPAQYLEAVESIDCGSRVVRGTGGGMPAYGSETSLSSRARAFLELYAIALFISYVVTTLGTKTWVVYIINYTLDMN

Homology

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BLAST of Carg07428 vs. NCBI nr
Match: KAG7036688.1 (hypothetical protein SDJN02_00308, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 311.2 bits (796), Expect = 5.1e-81
Identity = 161/161 (100.00%), Postives = 161/161 (100.00%), Query Frame = 0


Query: 1   MTPISLCGAAIILGINSNPVLAKDAPSKPSSENGIEESDTIGLRKVEDGSGKLEEAERFF 60
           MTPISLCGAAIILGINSNPVLAKDAPSKPSSENGIEESDTIGLRKVEDGSGKLEEAERFF
Sbjct: 1   MTPISLCGAAIILGINSNPVLAKDAPSKPSSENGIEESDTIGLRKVEDGSGKLEEAERFF 60

Query: 61  VSAIQEAKEGFGERDPHVASAFNNLRPAQYLEAVESIDCGSRVVRGTGGGMPAYGSETSL 120
           VSAIQEAKEGFGERDPHVASAFNNLRPAQYLEAVESIDCGSRVVRGTGGGMPAYGSETSL
Sbjct: 61  VSAIQEAKEGFGERDPHVASAFNNLRPAQYLEAVESIDCGSRVVRGTGGGMPAYGSETSL 120

Query: 121 SSRARAFLELYAIALFISYVVTTLGTKTWVVYIINYTLDMN 162
           SSRARAFLELYAIALFISYVVTTLGTKTWVVYIINYTLDMN
Sbjct: 121 SSRARAFLELYAIALFISYVVTTLGTKTWVVYIINYTLDMN 161

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BLAST of Carg07428 vs. NCBI nr
Match: KAG6606987.1 (Nephrocystin-3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 136.7 bits (343), Expect = 1.7e-28
Identity = 77/100 (77.00%), Postives = 77/100 (77.00%), Query Frame = 0


Query: 9   AAIILGINSNPVLAKDAPSKPSSENGIEESDTIGLRKVEDGS------------------ 68
           AAIILGINSNPVLAKDAPSKPSSENGIEESDTIGLRKVEDGS                  
Sbjct: 71  AAIILGINSNPVLAKDAPSKPSSENGIEESDTIGLRKVEDGSVISNLHTSKWRVFTDNAR 130

Query: 69  -----GKLEEAERFFVSAIQEAKEGFGERDPHVASAFNNL 86
                GKLEEAERFFVSAIQEAKEGFGERDPHVASAFNNL
Sbjct: 131 DLFLQGKLEEAERFFVSAIQEAKEGFGERDPHVASAFNNL 170

[Close]

BLAST of Carg07428 vs. NCBI nr
Match: XP_022948435.1 (uncharacterized protein LOC111452120 [Cucurbita moschata])

HSP 1 Score: 136.7 bits (343), Expect = 1.7e-28
Identity = 77/100 (77.00%), Postives = 77/100 (77.00%), Query Frame = 0


Query: 9   AAIILGINSNPVLAKDAPSKPSSENGIEESDTIGLRKVEDGS------------------ 68
           AAIILGINSNPVLAKDAPSKPSSENGIEESDTIGLRKVEDGS                  
Sbjct: 71  AAIILGINSNPVLAKDAPSKPSSENGIEESDTIGLRKVEDGSVISNLHTSKWRVFTDNAR 130

Query: 69  -----GKLEEAERFFVSAIQEAKEGFGERDPHVASAFNNL 86
                GKLEEAERFFVSAIQEAKEGFGERDPHVASAFNNL
Sbjct: 131 DLFLQGKLEEAERFFVSAIQEAKEGFGERDPHVASAFNNL 170

[Close]

BLAST of Carg07428 vs. NCBI nr
Match: XP_022997758.1 (uncharacterized protein LOC111492620 [Cucurbita maxima])

HSP 1 Score: 135.6 bits (340), Expect = 3.9e-28
Identity = 76/100 (76.00%), Postives = 77/100 (77.00%), Query Frame = 0


Query: 9   AAIILGINSNPVLAKDAPSKPSSENGIEESDTIGLRKVEDGS------------------ 68
           AAIILGINSNPVLAKDAPSKPSSENGIEESDTIGLRKVEDGS                  
Sbjct: 71  AAIILGINSNPVLAKDAPSKPSSENGIEESDTIGLRKVEDGSVISNLHTSKWRVFTDNAR 130

Query: 69  -----GKLEEAERFFVSAIQEAKEGFGERDPHVASAFNNL 86
                GKLEEAER+FVSAIQEAKEGFGERDPHVASAFNNL
Sbjct: 131 DLFLQGKLEEAERYFVSAIQEAKEGFGERDPHVASAFNNL 170

[Close]

BLAST of Carg07428 vs. NCBI nr
Match: XP_023525053.1 (uncharacterized protein LOC111788782 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 135.6 bits (340), Expect = 3.9e-28
Identity = 76/100 (76.00%), Postives = 77/100 (77.00%), Query Frame = 0


Query: 9   AAIILGINSNPVLAKDAPSKPSSENGIEESDTIGLRKVEDGS------------------ 68
           AAIILGINSNPVLAKDAPSKPSSENGIEESDTIGLRKVEDGS                  
Sbjct: 71  AAIILGINSNPVLAKDAPSKPSSENGIEESDTIGLRKVEDGSVISNLHTSKWRVFTDNAR 130

Query: 69  -----GKLEEAERFFVSAIQEAKEGFGERDPHVASAFNNL 86
                GKLEEAER+FVSAIQEAKEGFGERDPHVASAFNNL
Sbjct: 131 DLFLQGKLEEAERYFVSAIQEAKEGFGERDPHVASAFNNL 170

[Close]

BLAST of Carg07428 vs. ExPASy TrEMBL
Match: A0A6J1G9U8 (uncharacterized protein LOC111452120 OS=Cucurbita moschata OX=3662 GN=LOC111452120 PE=4 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 8.4e-29
Identity = 77/100 (77.00%), Postives = 77/100 (77.00%), Query Frame = 0


Query: 9   AAIILGINSNPVLAKDAPSKPSSENGIEESDTIGLRKVEDGS------------------ 68
           AAIILGINSNPVLAKDAPSKPSSENGIEESDTIGLRKVEDGS                  
Sbjct: 71  AAIILGINSNPVLAKDAPSKPSSENGIEESDTIGLRKVEDGSVISNLHTSKWRVFTDNAR 130

Query: 69  -----GKLEEAERFFVSAIQEAKEGFGERDPHVASAFNNL 86
                GKLEEAERFFVSAIQEAKEGFGERDPHVASAFNNL
Sbjct: 131 DLFLQGKLEEAERFFVSAIQEAKEGFGERDPHVASAFNNL 170

[Close]

BLAST of Carg07428 vs. ExPASy TrEMBL
Match: A0A6J1KEU5 (uncharacterized protein LOC111492620 OS=Cucurbita maxima OX=3661 GN=LOC111492620 PE=4 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 1.9e-28
Identity = 76/100 (76.00%), Postives = 77/100 (77.00%), Query Frame = 0


Query: 9   AAIILGINSNPVLAKDAPSKPSSENGIEESDTIGLRKVEDGS------------------ 68
           AAIILGINSNPVLAKDAPSKPSSENGIEESDTIGLRKVEDGS                  
Sbjct: 71  AAIILGINSNPVLAKDAPSKPSSENGIEESDTIGLRKVEDGSVISNLHTSKWRVFTDNAR 130

Query: 69  -----GKLEEAERFFVSAIQEAKEGFGERDPHVASAFNNL 86
                GKLEEAER+FVSAIQEAKEGFGERDPHVASAFNNL
Sbjct: 131 DLFLQGKLEEAERYFVSAIQEAKEGFGERDPHVASAFNNL 170

[Close]

BLAST of Carg07428 vs. ExPASy TrEMBL
Match: A0A5D3CQC2 (Kinesin light chain 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold363G00300 PE=4 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 9.6e-25
Identity = 64/88 (72.73%), Postives = 74/88 (84.09%), Query Frame = 0


Query: 9   AAIILGINSNPVLAKDAPSKPSSENGIEESDTIGLRKVEDGS-----------GKLEEAE 68
           AAIILGINSNPVLA++A  KPSSENGIE+ +T+GLRKVEDGS           G+LE+AE
Sbjct: 71  AAIILGINSNPVLAEEASFKPSSENGIEDGETVGLRKVEDGSVVSNLHTSKWRGRLEDAE 130

Query: 69  RFFVSAIQEAKEGFGERDPHVASAFNNL 86
           ++F+SAIQEAKEGFGERDPHVASAFNNL
Sbjct: 131 KYFISAIQEAKEGFGERDPHVASAFNNL 158

[Close]

BLAST of Carg07428 vs. ExPASy TrEMBL
Match: A0A6J1CQP6 (uncharacterized protein LOC111013309 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111013309 PE=4 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 2.1e-24
Identity = 69/100 (69.00%), Postives = 72/100 (72.00%), Query Frame = 0


Query: 9   AAIILGINSNPVLAKDAPSKPSSENGIEESDTIGLRKVEDGS------------------ 68
           AAIILGINSNPV AKD   KPSSENGIEE+DTIGLRKVEDGS                  
Sbjct: 71  AAIILGINSNPVFAKDTTFKPSSENGIEENDTIGLRKVEDGSVISNIHTSKWRVFTDTAR 130

Query: 69  -----GKLEEAERFFVSAIQEAKEGFGERDPHVASAFNNL 86
                G+LEEAER FVSA+QEAKEGFGERDPHVASAFNNL
Sbjct: 131 DLFLQGRLEEAERCFVSALQEAKEGFGERDPHVASAFNNL 170

[Close]

BLAST of Carg07428 vs. ExPASy TrEMBL
Match: A0A6J1CQT4 (kinesin light chain 1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111013309 PE=4 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 2.1e-24
Identity = 69/100 (69.00%), Postives = 72/100 (72.00%), Query Frame = 0


Query: 9   AAIILGINSNPVLAKDAPSKPSSENGIEESDTIGLRKVEDGS------------------ 68
           AAIILGINSNPV AKD   KPSSENGIEE+DTIGLRKVEDGS                  
Sbjct: 71  AAIILGINSNPVFAKDTTFKPSSENGIEENDTIGLRKVEDGSVISNIHTSKWRVFTDTAR 130

Query: 69  -----GKLEEAERFFVSAIQEAKEGFGERDPHVASAFNNL 86
                G+LEEAER FVSA+QEAKEGFGERDPHVASAFNNL
Sbjct: 131 DLFLQGRLEEAERCFVSALQEAKEGFGERDPHVASAFNNL 170

[Close]

BLAST of Carg07428 vs. TAIR 10
Match: AT5G37590.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 84.3 bits (207), Expect = 9.5e-17
Identity = 53/105 (50.48%), Postives = 59/105 (56.19%), Query Frame = 0


Query: 4   ISLCGAAIILGINSNPVLAKDAPSKPSSENGIEESDTIGLRKVEDGS------------- 63
           I L G A ILG   N VLA+D   K  S + ++ES   GL K+EDGS             
Sbjct: 64  IILSGQAAILGFCGNTVLAEDESMKSKSGDNMDESGNTGLEKIEDGSVVSNIHTSKWRVF 123

Query: 64  ----------GKLEEAERFFVSAIQEAKEGFGERDPHVASAFNNL 86
                     GKLE AER F SAIQEAKEGFGE+DPHVASA NNL
Sbjct: 124 TDSGRDYFFQGKLEPAERLFGSAIQEAKEGFGEKDPHVASACNNL 168

The following BLAST results are available for this feature:

BLAST of Carg07428 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7036688.1	5.1e-81	100.00	hypothetical protein SDJN02_00308, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KAG6606987.1	1.7e-28	77.00	Nephrocystin-3, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022948435.1	1.7e-28	77.00	uncharacterized protein LOC111452120 [Cucurbita moschata]	[more]
XP_022997758.1	3.9e-28	76.00	uncharacterized protein LOC111492620 [Cucurbita maxima]	[more]
XP_023525053.1	3.9e-28	76.00	uncharacterized protein LOC111788782 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg07428 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Carg07428 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G9U8	8.4e-29	77.00	uncharacterized protein LOC111452120 OS=Cucurbita moschata OX=3662 GN=LOC1114521...	[more]
A0A6J1KEU5	1.9e-28	76.00	uncharacterized protein LOC111492620 OS=Cucurbita maxima OX=3661 GN=LOC111492620...	[more]
A0A5D3CQC2	9.6e-25	72.73	Kinesin light chain 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold36...	[more]
A0A6J1CQP6	2.1e-24	69.00	uncharacterized protein LOC111013309 isoform X2 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1CQT4	2.1e-24	69.00	kinesin light chain 1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111013309 ...	[more]

BLAST of Carg07428 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G37590.1	9.5e-17	50.48	Tetratricopeptide repeat (TPR)-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR47689	TETRATRICOPEPTIDE REPEAT (TPR)-LIKE SUPERFAMILY PROTEIN	coord: 7..85

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg07428-RA	Carg07428-RA	mRNA