Carg07372 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg07372
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Pectinesterase
Location	Carg_Chr01: 1406330 .. 1412458 (+)
RNA-Seq Expression	Carg07372
Synteny	Carg07372

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAACATTACAATGACAGAATTTTGTTAATAACAACGTTGTTGTTGTTACTTTGTCTCAATGTTTCAAAAGCATTGGATTGTGAGTTGAATTTTAAAGATCCGAATAGAGTTGCACGTACTATTTTTGTTCATCAATTAGGGTCGGGAAATTTCAAAACAATCCAAAGTGCCATTGATTCGATCCCTCCGCAAAATAATCGATGGATTAGAATTCTAATCTCTCCTGGCGTATATCGGTAAGATATCATTTTATTTATTTACCTTTCTTCGTTTATGTTGCGTGGTTAAAAAGTCTAATTTTTTTATTTAAACGTGTGAAATCTCACATCAGTTGGAGAGGGGAACAAAACATTCCTTAAAGGATGTGGAAACTTCTTCTTGATAGACGCATTTTAAAACCGTGAAATTGATAATGATACGTAATGAGCCAGAACGGACAATATTTACTAGTGGTAACAATATACCGTAAATTACAGGGAAAAGGTGAGAATTCCCCCAGAAAAACCATGCATCTTTCTAGATGGGGTTGATAGAAATCTCACTGAAATTCAATGGAGTGACCATGAAACCACAGCTTCAAGTGCCACCTTCACTTTTTCTGCAGTAAATCTCGTGGTACGAGGCATCACATTTAAGGTACGAGTTCCGATGAGAATTAATTCAAGTATTGAATTTAAATTCTGAATCTTGAACATGGAGCGTAAATGAAATTGACAGAATACTTATAATACGCCGGGAAGTGTGACGTCACGTAATGATATAAAACCAGCACTTGCAGCTTTTGTAGACGGAGATAAAGCAGTCTTCCATCAGTGTGGCTTTTATGGTCTGCAAGATACTTTGTGGGACGGACCTGGTCGTCATCGATACACCGAATGCTACATTGAAGGTGTAGTCGATGTCATATCTGGTACTGGTCAATCTATGTACGAGGTTAGATTTTATATTGTTTTAGCTATATATATATATATAGGAACTAAACACTCCTTATAAGAGTGAAAATCTCTCCCTAGCCGACACATTTTAAGATTGTGAGGCTAAGGTGATACATAATGGGCCAAAACGGACAATATCTGTTAGCGGTGGGCTTTGGTTGTTACAAATGATATCAGACCTAGTCACCGTATGGTGTGCTAGTGAGGATGTTGGCCCCTAAGGGGTTGATTGTCAGATCCCATATTGGTTGTAGAGGAGAACGAAACATTCCTTATAAAGGTGTGGAAACCTCCGTTAGCAGACATGTTTTAAAATCATGAGGCTAACGACGATACGTAACGGACCAAAGCATACAATATCTGCTAGCAGTGGGTTTTAGCTCTTACAAATGTTATCAAAGCCAGTCATCAGAATGTGTGCTAGCGAGGACGCTGGGCCTCTAAGAGGGGTGGATTGTGAGATCTCAAATTGGTTGGAGAGGGAAACGATACATTCTTTATAAGGGTATGAAAAACTTCTCCCTAACAAACACGTTTTAAAACCGTGAGGCTGATGACGATACGTAACGAGCCAAAACGAATAGTATCTACTAGCGGTGGGTTTTGGCTATTACAAATGATGTCAGAGCCAATCACCGGATGGTGTGTTAGCGAGGACGCTAGGCTCCCAAGGAAGGTGGATAGTGAGATCTCACGTTAGTTGGAGAGGGGAACGAAACATTTTTATAAGGGTATGAAAATCTCTCCCTAGCATACGCATTTTAAAACCTTAAGAGGAACCTCGAAAGGTATCCGCTAGCCACGATACGATATGATTCGAATCCGGTATACCCACGTTTAAAGATTGAATTGGTTCGGAGTTGTGATTTAGTTTTGTTTATGTGTTATTTACTGCAGAAGTGTGTGATAAATATACCAATCAACGTATATGCTCCAATATTAGGACATGGTTACATAACAGCTCAAGGAAAGGAAGCTTCAAGCCATACCAATGGGTTTGTATTCATTGAGTGCTCAGTGATTGGAAGTGGAACTGTTTATCTTGGGAGGGCTTATAGACGTTTCTCCACAGTTATTTATCATAAGTCATTCCTATCATCTTGTATTAACCCTGTTGGTTGGCATCCTTGGACACAAGTAGGCCATGAGTATGTCTCCGCTTCTCCTTATAAATTTACTCGAACGAGTATCTAATGTTAGATGGATCGACATGCTCGACTAAATTATTTTGTTATATGATCTTGTAGGATGGATTTTACGTACGTGGAATCAAGTTGCACTGGACCAGGAGCAGACATTTCGAAGCGTGTGCCTTGGATTAAGAATCTTAATAAATATGATCTTAGACGTTTCATCGATATTTCTTTTATTGATCAAGATGGTTGGACTAGGAAGCTTCCTCAGTTTTAGAACTAAAATGACTTGGATCATCGTGTTGTAATAAGTGATGTGTGAAGTGTACTTAACATTTTCTCGGTTAATATGTTAAGTGTTAGGACATATATGCATCTGCATGATCTGTTTTGTAGTATGAATCAAATGAATAAATCGAGTAGCTACATAGTTCTATAATTTGTATGATATTTACTTTGTCCCCTACAACACTTCAGAAAACGTCATGACACTATTTCTTTTGCAAAGTTGGGGTGTGAGGAGTTGTGTGCGTTAGCTCCAATGACTATCTGCATCCGTGCCTCTTCATAGGATGAATCTCGGGTAATCCAACAAAACTATTAGGTTACACAAATCTAATCGCCCTCGTCCCTATCTCGATGATACTCATATCGAAATGGACACTCCACAAATACCGTATCAACTACTGTTGGCTTAAGAGGCTACCTTTGTCTTCCTAGGTTGTCGTGGTGGTCTTCTAGGTCCCTAGGGTGGTTTGGCAGCTCTGACTTGGATTAACATGATATCTAGGCTTCTCTGTTCAGACTCCTTGAACCTAGATAGGTCGAGAGCACTAACGACTAAACCTGCTGACTGTCGCTTATGTTTCTAAGGTGCTCGAACACTGGAGGCATTCCATCCTAGATCATAGTCTAGCCAAACTGACTTTAACACTCTAGTCTTAACCCGTTTCTACTACTTGCGCTCTACGGTGAGATCACACGTTCAATCACCTAGAGTTAATGTACATTAAATCATGCATACAGTCACACATGCTCAACGGGCGCTAACATCCATCAAGCACCCAGAGCACAGATCACAAATTTCTCATCATCTCTATGATTACTTACATGACTATCACGTGTCTTCCCAAGTTCAATTGCTCAGTAGAAAAGCCACCAAAATTCTGAAAATTCTCGGGAGAAGCCTAATTCAGGTCCGCCCAAGGTCTTCTAAGTTAGTAAGAGGGGCAAAATCCCCTCGGTTGAATTCGACTGGAATTTTTCAGTTTTTTCGCTATTTTCAATTTTATTTTTTCTCAAGTCCGTAGAGCTATCCCAAACATTGTTTCCTTCCACACTTCTTTGAAATTCAACCTTTAAGGCCCTCGATAAAATCTTTTAAGCCAAAAATTAAACAAAAAATTAGAAAAACCTACCTATTTGTTCAACTCGAGCATTACAATGCATTTGGTAACAAATCCTAGTTGGTATAAGATTTTTGAAAGTTGGGTCAGAGTCCACCATATAGGTTATATAGACGGGTTACAACGTAAACTTTAATGTGTATTTACTTGACAAAACAACTAACTCCCGTCACGATCAGATATACTTTAATAAAGGACAACAATTATATTATGTTTATGTAACGAGTTACCTATATATATATATATAGTATTTAAGGTGTCATTTATATAAGTTACCATAATAGAATTTATGCATTTCAACTATTCAAATGCCAACAAATTTAATTTGATATATAACTTGGATTTCATATCGCTTTACGTTTATTTCATCATCTCTTAATCATATCTTATTCACACACAAAAAAAAAAAAAAAAAAATTACAAAAGTCACATGCTCATAATTAATTTAATTCTCTCATGTTTATATATGTGTGTGTTATTACGTAAAAGGTCTTATGATTTGTTTGTTCCAAGAAATATGGAACAAATTTTGTTAATAACAACGTTGTTGATGTTACTTTGTCTCAATGTTTCAAAAGCATTAGATTGTGAGTTGAATCCTAATGATCCTAATAGAGTTGCATATACTATTGTTGTTGACAAATCAGGTTCGGGAAATTTCAGAACAATCCAAAGTGCGATTGATTCGATCCCTTCACAAAATAGCCGATGGATTAGAATTCAAATTTCTAGTGGAATATACTTGTAAGATATCGTTTCGTTCATATATCATTTTTCGTTCGTGTTGTGTGGCTAATTACTCATTCGTTTTTTATGATATTACCAAATTATTGATACGAGTCATAGATCGAAGTTATAGATAGAACAATTTGAGAAATTATTTGAGAAGATCATTGTGATGTAATAGGGAAAAGGTGCATATTTCGGAAGAAAAATCGTGCATCTTTCTAGATGGAGCTGGTAGAAGTTCCACGGAAATTCGATGGAATGACCATGCAACCACCGCTACAAGTGCCACCTTCACTTCTATTGCAGAAAATCTCGTAGTAAAAGGCATCACATTCAAGGTAGTATTGATATTATTTTTTTCATGTTGCAATATTTGTTAGCGAGGGGCTTTAGCTGTTACAGATAGTATCAGAGCTAGACATCAGACGGTGCGCTAACGAATACGGTAGACTTCAGGGAGGTGGATTGTGAGATCCCACATTGGTTGGAGAGTGTATTCCTCCCTAGTGGACATGTCTCTTCTTAGTCCAAATAAGAAAGATGATAATATTTGTTAGCGGTAAAATGACTTTTGCAGAATACATATAATGCGCCGGGAAGTGTGAAGAGACAAGAAGATATAGCACCAGCAGTTGCAGCTCTCATAGAAGGAGATAAAGGGATTTTCCATAAGTGTGGCTTTGTTGGTCTGCAAGATACCTTGTGGGATGGAAATGGTCGCCATCGCTATACCGAATGCTACATTGAAGGTGTAATCGATGTCATATCTGGCGCTGGTCAATCTATATACGAGGTTAACTTTAAACTATGTTTTACACGTTTTATACTGTATCACGGTTTTAAAATAAAAACACTCTTATAAAGAATGTTTCGTTCTCCTTATCCAATCGATGTAGGATCTTACAGTCCATCTCTTTTGGAGGCCAAGCGTCTTTGTTAGCTGGCTCTGATACTATTTGTAACAGCTCAAGTTCACCGCTAATAGATATTGTACGTTTTGACCCCTTACATATTGTCGTCAGCCTCACGGTTTTAAAATGCTTCTACTATAGAAAAGGTCTATCCCTACTCCAACTAATGCCTCACATTATTCGGTAACCTGGCTTTGATACCGTTTGTAATAGTCTAAGCCTGCCGTTGGTAAATATTATCCACTTTGACTCGTTACGTACCATCGTCAATCTCATGGTTTTAAAACACATCCGCTGGAAAGAGGTTTCCACACCCTTATAAGGAATGTTTCATTCCCCACTCCAACCGACGTGGGATCTCACAAGTATTCAATTGATTTGAAATCTTTTTCATTTACGTAAGTGTTCGAGTTAGCTAACATGCATACTTGGATTAATCTCATTACATAACAACTCGAGCCATTCAACAATACGTGCATTACACCTTAAGTTAGACATTGAATTGGTTTGGAGTAGTGATTATTGTGGTCGTGTTGTTTACTGCAGAACTGTGAGATAAATGTACCAGTCGACCTATATGCTCCAGTGATAGGACATGGTTACATAACAGCTCAAGGGAAGGAAGATTCAAGTCAAACCAATGGGTTTGTATTCATTGGATGCTCTGTGATTGGAAGTGGGACTGTTTATCTTGGGAGGGCTTACAGGCCTTTCTCCACAGTTATTTACTATTCATCTTTCCTATCAGCTTGTATTGACCCAGCTGGTTGGGATCCTTGGGAACAAGTTGGCCATGAGTAAGCCTTTTTTTTTCTTCCTGTTCTTCGCTTCTCGAGACGAGACATTAACGTTAATTGTTATGTGATGTGAATCTTGTAGGGCGAGTTTGACTTATGCAGAAACACATTGTGTTGGACTAGGAGCAGACACTTCGAAGTGTGTGTCTTGGCTTAAAAAACTTAGTATAGATGAGATTAGACACTTTACGAGTATTTCTTTCATTGATCAGGAA

mRNA sequence

ATGGAACATTACAATGACAGAATTTTGTTAATAACAACGTTGTTGTTGTTACTTTGTCTCAATGTTTCAAAAGCATTGGATTGTGAGTTGAATTTTAAAGATCCGAATAGAGTTGCACGTACTATTTTTAAGTGTGTGATAAATATACCAATCAACGTATATGCTCCAATATTAGGACATGGTTACATAACAGCTCAAGGAAAGGAAGCTTCAAGCCATACCAATGGGTTTGTATTCATTGAGTGCTCAGTGATTGGAAGTGGAACTGTTTATCTTGGGAGGGCTTATAGACGTTTCTCCACAGTTATTTATCATAAGTCATTCCTATCATCTTGTATTAACCCTGTTGGTTGGCATCCTTGGACACAAGTAGGCCATGAGTATGTCTCCGCTTCTCCTTATAAATTTACTCGAACGAGATGGATTTTACGTACGTGGAATCAAGTTGCACTGGACCAGGAGCAGACATTTCGAAGCGTGTGCCTTGGATTAAGAATCTTAATAAATATGATCTTAGACGTTTCATCGATATTTCTTTTATTGATCAAGATGAACTGTGAGATAAATGTACCAGTCGACCTATATGCTCCAGTGATAGGACATGGTTACATAACAGCTCAAGGGAAGGAAGATTCAAGTCAAACCAATGGGTTTGTATTCATTGGATGCTCTGTGATTGGAAGTGGGACTGTTTATCTTGGGAGGGCTTACAGGCCTTTCTCCACAGTTATTTACTATTCATCTTTCCTATCAGCTTGTATTGACCCAGCTGGTTGGGATCCTTGGGAACAAGTTGGCCATGAGGCGAGTTTGACTTATGCAGAAACACATTGTGTTGGACTAGGAGCAGACACTTCGAAGTGTGTGTCTTGGCTTAAAAAACTTAGTATAGATGAGATTAGACACTTTACGAGTATTTCTTTCATTGATCAGGAA

Coding sequence (CDS)

ATGGAACATTACAATGACAGAATTTTGTTAATAACAACGTTGTTGTTGTTACTTTGTCTCAATGTTTCAAAAGCATTGGATTGTGAGTTGAATTTTAAAGATCCGAATAGAGTTGCACGTACTATTTTTAAGTGTGTGATAAATATACCAATCAACGTATATGCTCCAATATTAGGACATGGTTACATAACAGCTCAAGGAAAGGAAGCTTCAAGCCATACCAATGGGTTTGTATTCATTGAGTGCTCAGTGATTGGAAGTGGAACTGTTTATCTTGGGAGGGCTTATAGACGTTTCTCCACAGTTATTTATCATAAGTCATTCCTATCATCTTGTATTAACCCTGTTGGTTGGCATCCTTGGACACAAGTAGGCCATGAGTATGTCTCCGCTTCTCCTTATAAATTTACTCGAACGAGATGGATTTTACGTACGTGGAATCAAGTTGCACTGGACCAGGAGCAGACATTTCGAAGCGTGTGCCTTGGATTAAGAATCTTAATAAATATGATCTTAGACGTTTCATCGATATTTCTTTTATTGATCAAGATGAACTGTGAGATAAATGTACCAGTCGACCTATATGCTCCAGTGATAGGACATGGTTACATAACAGCTCAAGGGAAGGAAGATTCAAGTCAAACCAATGGGTTTGTATTCATTGGATGCTCTGTGATTGGAAGTGGGACTGTTTATCTTGGGAGGGCTTACAGGCCTTTCTCCACAGTTATTTACTATTCATCTTTCCTATCAGCTTGTATTGACCCAGCTGGTTGGGATCCTTGGGAACAAGTTGGCCATGAGGCGAGTTTGACTTATGCAGAAACACATTGTGTTGGACTAGGAGCAGACACTTCGAAGTGTGTGTCTTGGCTTAAAAAACTTAGTATAGATGAGATTAGACACTTTACGAGTATTTCTTTCATTGATCAGGAA

Protein sequence

MEHYNDRILLITTLLLLLCLNVSKALDCELNFKDPNRVARTIFKCVINIPINVYAPILGHGYITAQGKEASSHTNGFVFIECSVIGSGTVYLGRAYRRFSTVIYHKSFLSSCINPVGWHPWTQVGHEYVSASPYKFTRTRWILRTWNQVALDQEQTFRSVCLGLRILINMILDVSSIFLLLIKMNCEINVPVDLYAPVIGHGYITAQGKEDSSQTNGFVFIGCSVIGSGTVYLGRAYRPFSTVIYYSSFLSACIDPAGWDPWEQVGHEASLTYAETHCVGLGADTSKCVSWLKKLSIDEIRHFTSISFIDQE

Homology

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BLAST of Carg07372 vs. NCBI nr
Match: KAG7036632.1 (putative pectinesterase 10, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 646.7 bits (1667), Expect = 1.0e-181
Identity = 312/312 (100.00%), Postives = 312/312 (100.00%), Query Frame = 0


Query: 1   MEHYNDRILLITTLLLLLCLNVSKALDCELNFKDPNRVARTIFKCVINIPINVYAPILGH 60
           MEHYNDRILLITTLLLLLCLNVSKALDCELNFKDPNRVARTIFKCVINIPINVYAPILGH
Sbjct: 1   MEHYNDRILLITTLLLLLCLNVSKALDCELNFKDPNRVARTIFKCVINIPINVYAPILGH 60

Query: 61  GYITAQGKEASSHTNGFVFIECSVIGSGTVYLGRAYRRFSTVIYHKSFLSSCINPVGWHP 120
           GYITAQGKEASSHTNGFVFIECSVIGSGTVYLGRAYRRFSTVIYHKSFLSSCINPVGWHP
Sbjct: 61  GYITAQGKEASSHTNGFVFIECSVIGSGTVYLGRAYRRFSTVIYHKSFLSSCINPVGWHP 120

Query: 121 WTQVGHEYVSASPYKFTRTRWILRTWNQVALDQEQTFRSVCLGLRILINMILDVSSIFLL 180
           WTQVGHEYVSASPYKFTRTRWILRTWNQVALDQEQTFRSVCLGLRILINMILDVSSIFLL
Sbjct: 121 WTQVGHEYVSASPYKFTRTRWILRTWNQVALDQEQTFRSVCLGLRILINMILDVSSIFLL 180

Query: 181 LIKMNCEINVPVDLYAPVIGHGYITAQGKEDSSQTNGFVFIGCSVIGSGTVYLGRAYRPF 240
           LIKMNCEINVPVDLYAPVIGHGYITAQGKEDSSQTNGFVFIGCSVIGSGTVYLGRAYRPF
Sbjct: 181 LIKMNCEINVPVDLYAPVIGHGYITAQGKEDSSQTNGFVFIGCSVIGSGTVYLGRAYRPF 240

Query: 241 STVIYYSSFLSACIDPAGWDPWEQVGHEASLTYAETHCVGLGADTSKCVSWLKKLSIDEI 300
           STVIYYSSFLSACIDPAGWDPWEQVGHEASLTYAETHCVGLGADTSKCVSWLKKLSIDEI
Sbjct: 241 STVIYYSSFLSACIDPAGWDPWEQVGHEASLTYAETHCVGLGADTSKCVSWLKKLSIDEI 300

Query: 301 RHFTSISFIDQE 313
           RHFTSISFIDQE
Sbjct: 301 RHFTSISFIDQE 312

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BLAST of Carg07372 vs. NCBI nr
Match: KAG6606930.1 (putative pectinesterase 55, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 290.8 bits (743), Expect = 1.4e-74
Identity = 178/340 (52.35%), Postives = 201/340 (59.12%), Query Frame = 0


Query: 6   DRILLITTLLLLLCLNVSKALDCELNFKDPNRVARTIFKCVINIPINVYAPILGHGYITA 65
           ++I LITTLL+LLCLNVSKALDCELN  DPNRVA TI          V        + T 
Sbjct: 2   EQISLITTLLMLLCLNVSKALDCELNPNDPNRVAYTI----------VVDKSGSGNFRTI 61

Query: 66  QGKEASSHTNGFVFIECSVIGSGTVYLGRAYRRFSTVIYHKSFLSSCINPVGW--HPWTQ 125
           Q    S  +    +I   +  S  +YL + +                   + W  H  T 
Sbjct: 62  QSAIDSIPSQNSRWIRIQI--SSGIYLEKVHISEEKSCIFLDGAGRSSTEIRWNDHATTA 121

Query: 126 VGHEYVSASPYKFTRTRWILRTWN---------------QVALDQEQTFRSVC--LGLR- 185
               + S +     +      T+N                  ++ ++     C  +GL+ 
Sbjct: 122 TSATFTSIAENLVVKGITFKNTYNAPGSVKRQEDIAPAVAALIEGDKGIFHKCGFVGLQD 181

Query: 186 -------------ILINMILDVSSIFLLLIKMNCEINVPVDLYAPVIGHGYITAQGKEDS 245
                          I  ++DV S     I  NCEINVPVDLYAPVIGHGYITAQGKEDS
Sbjct: 182 TLWDGNGRHRYTECYIEGVIDVISGAGQSIYENCEINVPVDLYAPVIGHGYITAQGKEDS 241

Query: 246 SQTNGFVFIGCSVIGSGTVYLGRAYRPFSTVIYYSSFLSACIDPAGWDPWEQVGHEASLT 305
           SQTNGFVFIGCSVIGSGTVYLGRAYRPFSTVIYYSSFLSACIDPAGWDPWEQVGHEASLT
Sbjct: 242 SQTNGFVFIGCSVIGSGTVYLGRAYRPFSTVIYYSSFLSACIDPAGWDPWEQVGHEASLT 301

Query: 306 YAETHCVGLGADTSKCVSWLKKLSIDEIRHFTSISFIDQE 313
            AETHCVGLGADTSK VSWLKKLSIDEIRHFTSISFIDQE
Sbjct: 302 CAETHCVGLGADTSKRVSWLKKLSIDEIRHFTSISFIDQE 329

[Close]

BLAST of Carg07372 vs. NCBI nr
Match: XP_022949036.1 (putative pectinesterase 52 [Cucurbita moschata])

HSP 1 Score: 281.6 bits (719), Expect = 8.4e-72
Identity = 170/312 (54.49%), Postives = 188/312 (60.26%), Query Frame = 0


Query: 6   DRILLITTLLLLLCLNVSKALDCELNFKDPNRVARTIFKCVINIPINV-----YAPILGH 65
           ++ILLITTLLLLLCLNVSKALDCELN  DPNRVA TI   V++   +V      AP +  
Sbjct: 2   EQILLITTLLLLLCLNVSKALDCELNPNDPNRVAYTI---VVDKSGSVKRQEDIAPAVA- 61

Query: 66  GYITAQGKEASSHTNGFVFIECSVIGSGTVYLGRAYRRFSTVIYHKSFLSSCINPVGWHP 125
                +G +   H  GFV ++       T++ G    R++                    
Sbjct: 62  --ALIEGDKGIFHKCGFVGLQ------DTLWDGNGRHRYT-------------------- 121

Query: 126 WTQVGHEYVSASPYKFTRTRWILRTWNQVALDQEQTFRSVCLGLRILINMILDVSSIFLL 185
                                                          I  + DV S    
Sbjct: 122 --------------------------------------------ECYIEGVTDVISGAGQ 181

Query: 186 LIKMNCEINVPVDLYAPVIGHGYITAQGKEDSSQTNGFVFIGCSVIGSGTVYLGRAYRPF 245
            I  NCEIN+PVDLYAPVIGHGYITAQGKEDSSQTNGFVFIGCSVIGSGTVYLGRAYRPF
Sbjct: 182 SIYENCEINIPVDLYAPVIGHGYITAQGKEDSSQTNGFVFIGCSVIGSGTVYLGRAYRPF 237

Query: 246 STVIYYSSFLSACIDPAGWDPWEQVGHEASLTYAETHCVGLGADTSKCVSWLKKLSIDEI 305
           STVIYYSSFLSACI+PAGWDPWEQVGHEASLTYAET CVG GADTSK VSWL+ LSIDEI
Sbjct: 242 STVIYYSSFLSACINPAGWDPWEQVGHEASLTYAETRCVGPGADTSKRVSWLQNLSIDEI 237

Query: 306 RHFTSISFIDQE 313
           RHFTSISFIDQE
Sbjct: 302 RHFTSISFIDQE 237

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BLAST of Carg07372 vs. NCBI nr
Match: XP_023524602.1 (LOW QUALITY PROTEIN: probable pectinesterase 55 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 275.0 bits (702), Expect = 7.9e-70
Identity = 175/338 (51.78%), Postives = 200/338 (59.17%), Query Frame = 0


Query: 6   DRILLITTLLLLLCLNVSKALDCELNFKDPNRVA-------------RTIFKCVINIPIN 65
           ++ILLITTLLLLLCLNVSKALDCELN  DPNRVA             RTI   + +IP  
Sbjct: 2   EQILLITTLLLLLCLNVSKALDCELNPNDPNRVAYTIVVDKSGSGNFRTIQSAIDSIPSQ 61

Query: 66  VYAPI---LGHGYITAQGKEASSHTNGFVFIECSVIGSGTVYLGRAYRRFSTVIYHKSFL 125
               I   +  G  +   K   S     +F+E    G+G       +   +T     +F 
Sbjct: 62  NSRWIRIQISSGIYSE--KVHISEEKSCIFLE----GAGRSSTEIRWNDHATTATSATFT 121

Query: 126 SSCINPVGWHPWTQVGHEYVSASPYKFTRTRWILRTWNQVALDQEQTFRSV-CLGLR--- 185
           S   N V       +  +     P    R   I      +    +  F     +GL+   
Sbjct: 122 SIAENLV----VKGITFKNTYNVPGSVKRQEDIAPAVAALIEGDKGIFHKCGFVGLQDTL 181

Query: 186 -----------ILINMILDVSSIFLLLIKMNCEINVPVDLYAPVIGHGYITAQGKEDSSQ 245
                        I  ++DV S     I  +CEIN+PVD YAPVIGHGYITAQGKEDSSQ
Sbjct: 182 WDGNGRHRYTECYIEGVIDVISGAGQSIYESCEINIPVDRYAPVIGHGYITAQGKEDSSQ 241

Query: 246 TNGFVFIGCSVIGSGTVYLGRAYRPFSTVIYYSSFLSACIDPAGWDPWEQVGHEASLTYA 305
           TNGFVFIGCSVIGSGTVYLGRAYRPFSTVIYYSSFLSACI+PAGWDPWEQVGHEASLTY 
Sbjct: 242 TNGFVFIGCSVIGSGTVYLGRAYRPFSTVIYYSSFLSACINPAGWDPWEQVGHEASLTYL 301

Query: 306 ETHCVGLGADTSKCVSWLKKLSIDEIRHFTSISFIDQE 313
           ET C+G GADTSK V WLKKLSIDEIR FTSISF+DQE
Sbjct: 302 ETRCIGPGADTSKRVPWLKKLSIDEIRRFTSISFVDQE 329

[Close]

BLAST of Carg07372 vs. NCBI nr
Match: XP_022157021.1 (uncharacterized protein LOC111023848 [Momordica charantia])

HSP 1 Score: 257.7 bits (657), Expect = 1.3e-64
Identity = 157/437 (35.93%), Postives = 206/437 (47.14%), Query Frame = 0


Query: 44  KCVINIPINVYAPILGHGYITAQGKEASSHTNGFVFIECSVIGSGTVYLGRAYRRFSTVI 103
           KC+IN+PI+ YAPIL +GYITAQGKE  +  +GFVF  CSV+G+G VYLGRAYR FSTVI
Sbjct: 207 KCMINVPIHQYAPILSYGYITAQGKENPNEGSGFVFNACSVVGNGRVYLGRAYRPFSTVI 266

Query: 104 YHKSFLSSCINPVGWHPWTQVGHE----YVS----------------------------- 163
           +H SFL+  I+P GW+PW QVGHE    Y                               
Sbjct: 267 FHNSFLAGFIDPAGWNPWGQVGHETSLTYAELNCRGPGANISRRVPWLKRLHRAEIRHFT 326

Query: 164 ----------------ASPYK--------------------------FTRTRWILRT--- 223
                            +PY+                          F  T+WI      
Sbjct: 327 DISFIDQEALDCQLNFRNPYRVAYSIYVSKKGRANFRTVQSAIDSIPFNSTQWIRVQIGP 386

Query: 224 ---WNQVAL--------------------------------------------------- 283
              W +V +                                                   
Sbjct: 387 GIYWEKVVIPAGKTCIFLDGADNRFTEIQWNDHQATATSATFTSFAENLLVKGITFKNTY 446

Query: 284 ----------------------DQEQTFRSVCLGLR--------------ILINMILDVS 313
                                 D+   +    +GL+                I  ++D+ 
Sbjct: 447 NTPGSVRRREDIEPALAVLIEGDKASFYECRFIGLQDTLWDGTGRHHFVNCYIEGVIDII 506

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BLAST of Carg07372 vs. ExPASy Swiss-Prot
Match: Q3E9D3 (Probable pectinesterase 55 OS=Arabidopsis thaliana OX=3702 GN=PME55 PE=2 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 2.0e-28
Identity = 61/130 (46.92%), Postives = 83/130 (63.85%), Query Frame = 0


Query: 182 IKMNCEINVPVDLYAPVIGHGYITAQGKEDSSQTNGFVFIGCSVIGSGTVYLGRAYRPFS 241
           I  +C I V      P +  GYITAQG+ +++  NGFVFI C V G G  YLGRA+RP+S
Sbjct: 191 IYQSCVIQVLGGQLGPGV-TGYITAQGRTNANDANGFVFINCLVHGFGKAYLGRAWRPYS 250

Query: 242 TVIYYSSFLSACIDPAGWDPWEQVGHEASLTYAETHCVGLGADTSKCVSWLKKLSIDEIR 301
            VI+Y+S L+  +DP GW  W   G+E  LTYAE  C G G++TS+   W+KKLS   ++
Sbjct: 251 RVIFYNSNLTDVVDPLGWWEWNYQGYEKQLTYAEHGCFGSGSNTSRRAKWVKKLSASAVQ 310

Query: 302 HFTSISFIDQ 312
           H   +SFI++
Sbjct: 311 HLADLSFINR 319

[Close]

BLAST of Carg07372 vs. ExPASy Swiss-Prot
Match: O64479 (Putative pectinesterase 10 OS=Arabidopsis thaliana OX=3702 GN=PME10 PE=2 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 4.5e-28
Identity = 58/127 (45.67%), Postives = 81/127 (63.78%), Query Frame = 0


Query: 186 CEINVPVDLYAPVIGHGYITAQGKEDSSQTNGFVFIGCSVIGSGTVYLGRAYRPFSTVIY 245
           C + + V +Y P   +G ITAQG++  +   GFVF  C+V+GSG   LGRA++ +S VI+
Sbjct: 203 CTLKLRVGIYPPNEVYGTITAQGRDSPTDKGGFVFKDCTVMGSGKALLGRAWKSYSRVIF 262

Query: 246 YSSFLSACIDPAGWDPWEQVGHEASLTYAETHCVGLGADTSKCVSWLKKLSIDEIRHFTS 305
           Y S  S  I P GWD W+  G E  +T+ E  C G+GADTSK V WL K S  ++  FT+
Sbjct: 263 YRSMFSDNILPIGWDAWKAKGQEGHITFVEFGCTGVGADTSKRVPWLTKASEKDVLQFTN 322

Query: 306 ISFIDQE 313
           ++FID+E
Sbjct: 323 LTFIDEE 329

[Close]

BLAST of Carg07372 vs. ExPASy Swiss-Prot
Match: Q4PSQ5 (Probable pectinesterase 66 OS=Arabidopsis thaliana OX=3702 GN=PME66 PE=2 SV=2)

HSP 1 Score: 121.7 bits (304), Expect = 1.5e-26
Identity = 59/127 (46.46%), Postives = 77/127 (60.63%), Query Frame = 0


Query: 186 CEINVPVDLYAPVIGHGYITAQGKEDSSQTNGFVFIGCSVIGSGTVYLGRAYRPFSTVIY 245
           C +N+ + +YAP   +G ITA  +   S   GFVF  C+V G G   LGRA+   + VI+
Sbjct: 198 CTLNMTLGIYAPDNPYGTITAHQRPSPSDEGGFVFSDCTVTGVGKTLLGRAWGSNARVIF 257

Query: 246 YSSFLSACIDPAGWDPWEQVGHEASLTYAETHCVGLGADTSKCVSWLKKLSIDEIRHFTS 305
             S LS  + P GWD W   G+E  LT+ E  C G GADTS+ V WLKKLS+ E+  F S
Sbjct: 258 DRSRLSDVVLPIGWDAWRAKGNERDLTFVEAGCTGAGADTSQRVPWLKKLSLSEVDGFAS 317

Query: 306 ISFIDQE 313
           +SFIDQ+
Sbjct: 318 VSFIDQD 324

[Close]

BLAST of Carg07372 vs. ExPASy Swiss-Prot
Match: O04953 (Putative pectinesterase 52 OS=Arabidopsis thaliana OX=3702 GN=PME52 PE=2 SV=2)

HSP 1 Score: 120.2 bits (300), Expect = 4.3e-26
Identity = 60/129 (46.51%), Postives = 78/129 (60.47%), Query Frame = 0


Query: 185 NCEINVPVDLYAPVIGHGYITAQGKEDSSQTNGFVFIGCSVIGSGTVYLGRAYRPFSTVI 244
           +C IN      A  +  GYITAQG+   S  +GFVF+  SV GS +VYLGRAY PFS VI
Sbjct: 156 DCHINATAGALASKVSFGYITAQGRSSDSDPSGFVFLRGSVSGSTSVYLGRAYGPFSRVI 215

Query: 245 YYSSFLSACIDPAGWDPWEQVGHEASLTYAETHCVGLGADTSKCVSWLKKL-SIDEIRHF 304
           +  + LS+ + P GW  W   G+E S TYAE  C G G+D S+ V W+ KL S    + F
Sbjct: 216 FIQTDLSSVVHPEGWYSWHYGGYEMSFTYAEVECKGAGSDMSRRVPWIDKLHSFYTKQQF 275

Query: 305 TSISFIDQE 313
           +  +FIDQ+
Sbjct: 276 SISNFIDQD 284

[Close]

BLAST of Carg07372 vs. ExPASy Swiss-Prot
Match: Q4PSN0 (Probable pectinesterase 29 OS=Arabidopsis thaliana OX=3702 GN=PME29 PE=2 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 4.7e-25
Identity = 57/130 (43.85%), Postives = 79/130 (60.77%), Query Frame = 0


Query: 182 IKMNCEINVPVDLYAPVIGHGYITAQGKEDSSQTNGFVFIGCSVIGSGTVYLGRAYRPFS 241
           I  +C I V      P +  GYITAQG+ +    NGF+FI C V G+G  +LGR +R +S
Sbjct: 196 IYQSCVIQVLGGQLEPGLA-GYITAQGRTNPYDANGFIFINCLVYGTGMAFLGRPWRGYS 255

Query: 242 TVIYYSSFLSACIDPAGWDPWEQVGHEASLTYAETHCVGLGADTSKCVSWLKKLSIDEIR 301
            VI+Y+S L+  + P GWD W  VGHE  L +AE  C G GA+  + V W+KKLS   I+
Sbjct: 256 RVIFYNSNLTDVVVPEGWDAWNFVGHENQLVFAEHGCFGSGANIGRRVKWVKKLSESAIQ 315

Query: 302 HFTSISFIDQ 312
           +   +SFI++
Sbjct: 316 NLADLSFINR 324

[Close]

BLAST of Carg07372 vs. ExPASy TrEMBL
Match: A0A6J1GAV4 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111452500 PE=4 SV=1)

HSP 1 Score: 281.6 bits (719), Expect = 4.1e-72
Identity = 170/312 (54.49%), Postives = 188/312 (60.26%), Query Frame = 0


Query: 6   DRILLITTLLLLLCLNVSKALDCELNFKDPNRVARTIFKCVINIPINV-----YAPILGH 65
           ++ILLITTLLLLLCLNVSKALDCELN  DPNRVA TI   V++   +V      AP +  
Sbjct: 2   EQILLITTLLLLLCLNVSKALDCELNPNDPNRVAYTI---VVDKSGSVKRQEDIAPAVA- 61

Query: 66  GYITAQGKEASSHTNGFVFIECSVIGSGTVYLGRAYRRFSTVIYHKSFLSSCINPVGWHP 125
                +G +   H  GFV ++       T++ G    R++                    
Sbjct: 62  --ALIEGDKGIFHKCGFVGLQ------DTLWDGNGRHRYT-------------------- 121

Query: 126 WTQVGHEYVSASPYKFTRTRWILRTWNQVALDQEQTFRSVCLGLRILINMILDVSSIFLL 185
                                                          I  + DV S    
Sbjct: 122 --------------------------------------------ECYIEGVTDVISGAGQ 181

Query: 186 LIKMNCEINVPVDLYAPVIGHGYITAQGKEDSSQTNGFVFIGCSVIGSGTVYLGRAYRPF 245
            I  NCEIN+PVDLYAPVIGHGYITAQGKEDSSQTNGFVFIGCSVIGSGTVYLGRAYRPF
Sbjct: 182 SIYENCEINIPVDLYAPVIGHGYITAQGKEDSSQTNGFVFIGCSVIGSGTVYLGRAYRPF 237

Query: 246 STVIYYSSFLSACIDPAGWDPWEQVGHEASLTYAETHCVGLGADTSKCVSWLKKLSIDEI 305
           STVIYYSSFLSACI+PAGWDPWEQVGHEASLTYAET CVG GADTSK VSWL+ LSIDEI
Sbjct: 242 STVIYYSSFLSACINPAGWDPWEQVGHEASLTYAETRCVGPGADTSKRVSWLQNLSIDEI 237

Query: 306 RHFTSISFIDQE 313
           RHFTSISFIDQE
Sbjct: 302 RHFTSISFIDQE 237

[Close]

BLAST of Carg07372 vs. ExPASy TrEMBL
Match: A0A6J1DWQ8 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111023848 PE=4 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 6.3e-65
Identity = 157/437 (35.93%), Postives = 206/437 (47.14%), Query Frame = 0


Query: 44  KCVINIPINVYAPILGHGYITAQGKEASSHTNGFVFIECSVIGSGTVYLGRAYRRFSTVI 103
           KC+IN+PI+ YAPIL +GYITAQGKE  +  +GFVF  CSV+G+G VYLGRAYR FSTVI
Sbjct: 207 KCMINVPIHQYAPILSYGYITAQGKENPNEGSGFVFNACSVVGNGRVYLGRAYRPFSTVI 266

Query: 104 YHKSFLSSCINPVGWHPWTQVGHE----YVS----------------------------- 163
           +H SFL+  I+P GW+PW QVGHE    Y                               
Sbjct: 267 FHNSFLAGFIDPAGWNPWGQVGHETSLTYAELNCRGPGANISRRVPWLKRLHRAEIRHFT 326

Query: 164 ----------------ASPYK--------------------------FTRTRWILRT--- 223
                            +PY+                          F  T+WI      
Sbjct: 327 DISFIDQEALDCQLNFRNPYRVAYSIYVSKKGRANFRTVQSAIDSIPFNSTQWIRVQIGP 386

Query: 224 ---WNQVAL--------------------------------------------------- 283
              W +V +                                                   
Sbjct: 387 GIYWEKVVIPAGKTCIFLDGADNRFTEIQWNDHQATATSATFTSFAENLLVKGITFKNTY 446

Query: 284 ----------------------DQEQTFRSVCLGLR--------------ILINMILDVS 313
                                 D+   +    +GL+                I  ++D+ 
Sbjct: 447 NTPGSVRRREDIEPALAVLIEGDKASFYECRFIGLQDTLWDGTGRHHFVNCYIEGVIDII 506

[Close]

BLAST of Carg07372 vs. ExPASy TrEMBL
Match: A0A1S4DWQ1 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103490078 PE=4 SV=1)

HSP 1 Score: 204.1 bits (518), Expect = 8.3e-49
Identity = 139/350 (39.71%), Postives = 189/350 (54.00%), Query Frame = 0


Query: 1   MEHYNDRI-LLITTLLLLLC-LNVSKALD-CELNFKDPNRVARTIFKCVINIPINVYAPI 60
           MEHY+  I LLITTLLLL C +NVSKALD CELN+ DP++VA TI               
Sbjct: 1   MEHYSKEITLLITTLLLLFCHVNVSKALDYCELNYNDPHKVAYTI-----------VVDK 60

Query: 61  LGHG-YITAQG--KEASSHTNGFVFIECS-----VIGSGTVYLGRAYRRFSTVIYHKSFL 120
            G G + T Q       S +N ++ I+ S       GSG       +   +T     +F 
Sbjct: 61  FGRGNFHTIQSAIDSVPSQSNRWIRIQISPGIYTYDGSGFRVTEIQWNDHATTATSPTFT 120

Query: 121 SSCINPVGW-----HPWTQVG-----HEYVSASPYKFTRTRWILRTWNQVAL-DQEQTFR 180
           S   N V       + +  +G      +   A        + I      + L D      
Sbjct: 121 SYAQNLVVQGITFRNTYNALGSVTRREDIKPAIAALIQGDKAIFHKCGFIGLQDTLWDGP 180

Query: 181 SVCLGLRILINMILDVSS----------------IFLLLIKMNCEINVPVDLYAPVIGHG 240
              L  +  I  ++DV S                +F+L +   C IN+P++ YAP++  G
Sbjct: 181 GRHLFTQCYIEGVIDVISGFGHQIEVLIFFLFVCLFVLCLTQECVINIPINAYAPLLNEG 240

Query: 241 YITAQGKEDSSQTNGFVFIGCSVIGSGTVYLGRAYRPFSTVIYYSSFLSACIDPAGWDPW 300
           +ITAQGKE+ S+++GFVFI C V GSG V+LGRAYRPFSTVI++  FL +CIDPAGW+ W
Sbjct: 241 FITAQGKENPSESSGFVFIRCIVKGSGKVFLGRAYRPFSTVIFHLCFLPSCIDPAGWNSW 300

Query: 301 EQVGHEASLTYAETHCVGLGADTSKCVSWLKKLSIDEIRHFTSISFIDQE 313
           +QVGHE++LTY+ET C+G GADTS  V WLK+L + +IR FT IS+ID +
Sbjct: 301 QQVGHESNLTYSETRCIGPGADTSSRVPWLKRLDVYQIRRFTDISYIDPQ 339

[Close]

BLAST of Carg07372 vs. ExPASy TrEMBL
Match: A0A0A0LB12 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G536660 PE=4 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 4.3e-45
Identity = 134/361 (37.12%), Postives = 187/361 (51.80%), Query Frame = 0


Query: 1   MEHYNDR---ILLITTLLLLLCLNVSKAL-DCELNFKDPNRVARTIFKCVINIPINVYAP 60
           MEHY  +   + +ITTL++L C+ VSKAL DCELN+ DP++VA TI              
Sbjct: 1   MEHYYSKEISLFIITTLVVLFCVKVSKALDDCELNYNDPHKVAYTI-----------VVD 60

Query: 61  ILGHG-YITAQGKEASSHTNGFVFIECSVIGSGTVYLGRAYRRFSTVIYHKS--FLSSC- 120
            LG G + T Q    S  +    +I   +  S  +Y  +      T+   KS  FL    
Sbjct: 61  KLGKGNFTTIQSAINSVPSQSTKWIRIQI--SPGIYTEKV-----TIPSEKSCIFLDGSG 120

Query: 121 --INPVGWHPWTQVGHEYVSASPYKFTRTRWIL--------RTWN--------------- 180
             +  + W+      HE  +ASP  FT +   L         T+N               
Sbjct: 121 LQVTEIHWN-----DHETTAASP-TFTASAQNLVVQGITFRNTYNARGSVMRREDIKPAL 180

Query: 181 QVALDQEQTFRSVC--LGL--------------RILINMILDVSSIFLLLIKMNCEINVP 240
              +  ++     C  +GL              +  I  ++DV S F   I   C IN+P
Sbjct: 181 AALIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIP 240

Query: 241 VDLYAPVIGHGYITAQGKEDSSQTNGFVFIGCSVIGSGTVYLGRAYRPFSTVIYYSSFLS 300
           V+ YAP++  G+ITAQGKE+ ++++GFVF+ C V GSG V+LGRAYRPFSTVI++  FL 
Sbjct: 241 VNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLP 300

Query: 301 ACIDPAGWDPWEQVGHEASLTYAETHCVGLGADTSKCVSWLKKLSIDEIRHFTSISFIDQ 313
           +CI+PAGW+ W Q GHE+ LTY+ET C+G GADTS  V W+ +L    IR FT IS+ID 
Sbjct: 301 SCINPAGWNSWLQAGHESDLTYSETRCIGPGADTSSRVPWVNRLDAFHIRSFTDISYIDP 337

[Close]

BLAST of Carg07372 vs. ExPASy TrEMBL
Match: A0A5D3DA76 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold237G00550 PE=4 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 4.0e-43
Identity = 85/145 (58.62%), Postives = 112/145 (77.24%), Query Frame = 0


Query: 168 INMILDVSSIFLLLIKMNCEINVPVDLYAPVIGHGYITAQGKEDSSQTNGFVFIGCSVIG 227
           I  ++DV S F   I   C IN+P++ YAP++  G+ITAQGKE+ S+++GFVFI C V G
Sbjct: 99  IEGVIDVISGFGQSIYKECVINIPINAYAPLLNEGFITAQGKENPSESSGFVFIRCIVKG 158

Query: 228 SGTVYLGRAYRPFSTVIYYSSFLSACIDPAGWDPWEQVGHEASLTYAETHCVGLGADTSK 287
           SG V+LGRAYRPFSTVI++  FL +CIDPAGW+ W+QVGHE++LTY+ET C+G GADTS 
Sbjct: 159 SGKVFLGRAYRPFSTVIFHLCFLPSCIDPAGWNSWQQVGHESNLTYSETRCIGPGADTSS 218

Query: 288 CVSWLKKLSIDEIRHFTSISFIDQE 313
            V WLK+L + +IR FT IS+ID +
Sbjct: 219 RVPWLKRLDVYQIRRFTDISYIDPQ 243

[Close]

BLAST of Carg07372 vs. TAIR 10
Match: AT5G18990.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 127.9 bits (320), Expect = 1.5e-29
Identity = 61/130 (46.92%), Postives = 83/130 (63.85%), Query Frame = 0


Query: 182 IKMNCEINVPVDLYAPVIGHGYITAQGKEDSSQTNGFVFIGCSVIGSGTVYLGRAYRPFS 241
           I  +C I V      P +  GYITAQG+ +++  NGFVFI C V G G  YLGRA+RP+S
Sbjct: 191 IYQSCVIQVLGGQLGPGV-TGYITAQGRTNANDANGFVFINCLVHGFGKAYLGRAWRPYS 250

Query: 242 TVIYYSSFLSACIDPAGWDPWEQVGHEASLTYAETHCVGLGADTSKCVSWLKKLSIDEIR 301
            VI+Y+S L+  +DP GW  W   G+E  LTYAE  C G G++TS+   W+KKLS   ++
Sbjct: 251 RVIFYNSNLTDVVDPLGWWEWNYQGYEKQLTYAEHGCFGSGSNTSRRAKWVKKLSASAVQ 310

Query: 302 HFTSISFIDQ 312
           H   +SFI++
Sbjct: 311 HLADLSFINR 319

[Close]

BLAST of Carg07372 vs. TAIR 10
Match: AT2G19150.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 126.7 bits (317), Expect = 3.2e-29
Identity = 58/127 (45.67%), Postives = 81/127 (63.78%), Query Frame = 0


Query: 186 CEINVPVDLYAPVIGHGYITAQGKEDSSQTNGFVFIGCSVIGSGTVYLGRAYRPFSTVIY 245
           C + + V +Y P   +G ITAQG++  +   GFVF  C+V+GSG   LGRA++ +S VI+
Sbjct: 203 CTLKLRVGIYPPNEVYGTITAQGRDSPTDKGGFVFKDCTVMGSGKALLGRAWKSYSRVIF 262

Query: 246 YSSFLSACIDPAGWDPWEQVGHEASLTYAETHCVGLGADTSKCVSWLKKLSIDEIRHFTS 305
           Y S  S  I P GWD W+  G E  +T+ E  C G+GADTSK V WL K S  ++  FT+
Sbjct: 263 YRSMFSDNILPIGWDAWKAKGQEGHITFVEFGCTGVGADTSKRVPWLTKASEKDVLQFTN 322

Query: 306 ISFIDQE 313
           ++FID+E
Sbjct: 323 LTFIDEE 329

[Close]

BLAST of Carg07372 vs. TAIR 10
Match: AT2G47280.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 121.7 bits (304), Expect = 1.0e-27
Identity = 59/127 (46.46%), Postives = 77/127 (60.63%), Query Frame = 0


Query: 186 CEINVPVDLYAPVIGHGYITAQGKEDSSQTNGFVFIGCSVIGSGTVYLGRAYRPFSTVIY 245
           C +N+ + +YAP   +G ITA  +   S   GFVF  C+V G G   LGRA+   + VI+
Sbjct: 198 CTLNMTLGIYAPDNPYGTITAHQRPSPSDEGGFVFSDCTVTGVGKTLLGRAWGSNARVIF 257

Query: 246 YSSFLSACIDPAGWDPWEQVGHEASLTYAETHCVGLGADTSKCVSWLKKLSIDEIRHFTS 305
             S LS  + P GWD W   G+E  LT+ E  C G GADTS+ V WLKKLS+ E+  F S
Sbjct: 258 DRSRLSDVVLPIGWDAWRAKGNERDLTFVEAGCTGAGADTSQRVPWLKKLSLSEVDGFAS 317

Query: 306 ISFIDQE 313
           +SFIDQ+
Sbjct: 318 VSFIDQD 324

[Close]

BLAST of Carg07372 vs. TAIR 10
Match: AT5G26810.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 120.2 bits (300), Expect = 3.0e-27
Identity = 60/129 (46.51%), Postives = 78/129 (60.47%), Query Frame = 0


Query: 185 NCEINVPVDLYAPVIGHGYITAQGKEDSSQTNGFVFIGCSVIGSGTVYLGRAYRPFSTVI 244
           +C IN      A  +  GYITAQG+   S  +GFVF+  SV GS +VYLGRAY PFS VI
Sbjct: 156 DCHINATAGALASKVSFGYITAQGRSSDSDPSGFVFLRGSVSGSTSVYLGRAYGPFSRVI 215

Query: 245 YYSSFLSACIDPAGWDPWEQVGHEASLTYAETHCVGLGADTSKCVSWLKKL-SIDEIRHF 304
           +  + LS+ + P GW  W   G+E S TYAE  C G G+D S+ V W+ KL S    + F
Sbjct: 216 FIQTDLSSVVHPEGWYSWHYGGYEMSFTYAEVECKGAGSDMSRRVPWIDKLHSFYTKQQF 275

Query: 305 TSISFIDQE 313
           +  +FIDQ+
Sbjct: 276 SISNFIDQD 284

[Close]

BLAST of Carg07372 vs. TAIR 10
Match: AT3G24130.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 116.7 bits (291), Expect = 3.3e-26
Identity = 57/130 (43.85%), Postives = 79/130 (60.77%), Query Frame = 0


Query: 182 IKMNCEINVPVDLYAPVIGHGYITAQGKEDSSQTNGFVFIGCSVIGSGTVYLGRAYRPFS 241
           I  +C I V      P +  GYITAQG+ +    NGF+FI C V G+G  +LGR +R +S
Sbjct: 196 IYQSCVIQVLGGQLEPGLA-GYITAQGRTNPYDANGFIFINCLVYGTGMAFLGRPWRGYS 255

Query: 242 TVIYYSSFLSACIDPAGWDPWEQVGHEASLTYAETHCVGLGADTSKCVSWLKKLSIDEIR 301
            VI+Y+S L+  + P GWD W  VGHE  L +AE  C G GA+  + V W+KKLS   I+
Sbjct: 256 RVIFYNSNLTDVVVPEGWDAWNFVGHENQLVFAEHGCFGSGANIGRRVKWVKKLSESAIQ 315

Query: 302 HFTSISFIDQ 312
           +   +SFI++
Sbjct: 316 NLADLSFINR 324

The following BLAST results are available for this feature:

BLAST of Carg07372 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7036632.1	1.0e-181	100.00	putative pectinesterase 10, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6606930.1	1.4e-74	52.35	putative pectinesterase 55, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022949036.1	8.4e-72	54.49	putative pectinesterase 52 [Cucurbita moschata]	[more]
XP_023524602.1	7.9e-70	51.78	LOW QUALITY PROTEIN: probable pectinesterase 55 [Cucurbita pepo subsp. pepo]	[more]
XP_022157021.1	1.3e-64	35.93	uncharacterized protein LOC111023848 [Momordica charantia]	[more]

BLAST of Carg07372 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q3E9D3	2.0e-28	46.92	Probable pectinesterase 55 OS=Arabidopsis thaliana OX=3702 GN=PME55 PE=2 SV=1	[more]
O64479	4.5e-28	45.67	Putative pectinesterase 10 OS=Arabidopsis thaliana OX=3702 GN=PME10 PE=2 SV=1	[more]
Q4PSQ5	1.5e-26	46.46	Probable pectinesterase 66 OS=Arabidopsis thaliana OX=3702 GN=PME66 PE=2 SV=2	[more]
O04953	4.3e-26	46.51	Putative pectinesterase 52 OS=Arabidopsis thaliana OX=3702 GN=PME52 PE=2 SV=2	[more]
Q4PSN0	4.7e-25	43.85	Probable pectinesterase 29 OS=Arabidopsis thaliana OX=3702 GN=PME29 PE=2 SV=1	[more]

BLAST of Carg07372 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GAV4	4.1e-72	54.49	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111452500 PE=4 SV=1	[more]
A0A6J1DWQ8	6.3e-65	35.93	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111023848 PE=4 SV=1	[more]
A0A1S4DWQ1	8.3e-49	39.71	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103490078 PE=4 SV=1	[more]
A0A0A0LB12	4.3e-45	37.12	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G536660 PE=4 SV=1	[more]
A0A5D3DA76	4.0e-43	58.62	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold237G00550...	[more]

BLAST of Carg07372 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G18990.1	1.5e-29	46.92	Pectin lyase-like superfamily protein	[more]
AT2G19150.1	3.2e-29	45.67	Pectin lyase-like superfamily protein	[more]
AT2G47280.1	1.0e-27	46.46	Pectin lyase-like superfamily protein	[more]
AT5G26810.1	3.0e-27	46.51	Pectin lyase-like superfamily protein	[more]
AT3G24130.1	3.3e-26	43.85	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 61..123 e-value: 3.5E-11 score: 42.4 coord: 202..311 e-value: 1.4E-20 score: 73.2
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 181..312 e-value: 1.3E-28 score: 102.3
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 37..158 e-value: 7.7E-18 score: 66.9
None	No IPR available	PANTHER	PTHR31321:SF82	PECTINESTERASE	coord: 42..127
None	No IPR available	PANTHER	PTHR31321	ACYL-COA THIOESTER HYDROLASE YBHC-RELATED	coord: 42..127
None	No IPR available	PANTHER	PTHR31321	ACYL-COA THIOESTER HYDROLASE YBHC-RELATED	coord: 183..312
None	No IPR available	PANTHER	PTHR31321:SF82	PECTINESTERASE	coord: 183..312
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 60..122
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 201..311

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg07372-RA	Carg07372-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
molecular_function	GO:0030599	pectinesterase activity