Carg07321 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg07321
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	ATP synthase subunit O, mitochondrial-like
Location	Carg_Chr01: 1099534 .. 1101635 (+)
RNA-Seq Expression	Carg07321
Synteny	Carg07321

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCATGTTTAATCGAGGACTAAACAAATTTCATGAGGTATTTGCAGATTCTATTATCAGTTTTCCTTCTGAATCGGTTAATGCTGGTGTAAATAAAGCTGGTTTCTTGACCGTTTCTCTGATTTTGTATTGAGTATGGTTTATCACTTCAATAATGTGGTTATCAACTGGCTGCAGCTCTCAAGGACTTATGCCGCTACATCAGGGAAACATGAACAAAAGGTGAAGGTAGGTGAACAATTACTTTGGTTTCCTCATCATATTTTGTTTTCCGAATAGATTTTCAGAATTACTATTAGATATCCTCTTTTGAACTCATAGTGCATGCGCCAGGTATGTTTCTTTCTGGAATTCTCATTTACAAGCGTGCTTTCTTATTTGCTGCATTTCAGGTGCCTCTTGCTTTGTTTGGAGGGTCTGGAAACTATGCCTCTGCTTTGTATATAGCTGCAATAAAAGCTAATTCTCTAGACAAGGTGGAGTCTGAGCTTTTTAACCTCGTTGAGGCTATTAAGAAAAGTCCTATATTTGCTCAGTTCACAATGGACCTGTCAGTTCCAGCGGAGACTAGAGTTAAAGCTATCAATGAAATTTCTGCTGAAGCAAAATTTTCAGACGTTGTTGAGAACTTTTTGGGTAGGATTGTTTACACTTTTTTAGTTGTGCGGATGAAAAATGTATTTTTCTTTTGGTTTTTGATATCAGTTATTACAATGCCTCCGTCTCACAATTGGGTCCACATCATGCTGAACAGTCTGTCTGTTGATGTGTATTTCTTTTTCTATGCCAATTGTCAAATCTTAACTGCATCATTTCTTGTCCTATATAGATTTGGCTTGAGAGGTTTGAGGATTTTTGCCTTTCATGATTGAAGAAGTGAGTTTTATTCTATAGGATTTTTGAGATGGTTGATTCTGTTTTTTTTATTCCCTTGACTCAAGCAGTTACATTTGGTTCTAATGGCTGAAGTACTCATGCTGTGCAATCATTGATACTGGCAATTAAAATGCATCAATTCCAGGCTTACATGCATTCTCTTACCTGGAATTATTCTGATTATTTATTGTAGTTGTGTTGGCTGAGAATGGGAGGCTGAGATATATAGATAGCATAGCAAAGAGATTTTTGGAGTTGACTATGGCTCATAAGGGAGAAGTTAAAGCAGTTGTCACAACTGTTATTGTAAGTTTCTCTTTCACTTTCACTTTCCATTTTGTTTTATTTTATTTTATTTTTAAATCTAGGTAAAAATCAGATTCTTATCTATGAACTTCAGCGTGCAATGTGATTGAATACGTTTAGCATAAACTTATGCAAAAGTGATATTTATGTATAAAAGTATGACCTATTTAAATTCAGCAACTGATTTGTGGATGTCGTGGGATGCCTTTTCTATGTGTCTTGGGAGCCTTGTCAAATTCTTTACTTCCATTTTTATTTCTATTTTTAGCTTACTGATACTGGCAATTCGTTGGTCACGGATGAAAATGTCAAGAGACAATACTTGAAGTAAATGTAATGACATTTAAAATTAGAGGGTTTTTATTGAAGTACTTCTCGATGCACATGATAACTGATTGGTTTTTTACCAGCCCATTCCACCACAAGAAGAGAACGAACTGAAGGAGACATTTGCAAGATATTATTGGACAAGGCAAGAAGGTTAAGCTCGAGCAAAAGGTACGTCGGTTGGAAATGTTTTGGGTTTTGAATTTTGTGAATGTAGCTGCATGCCTTTTGAGAAATTCTAACCGTGCTTTTGTATTTGCCAGATTGATCCCAGCATACTTGGTGGTCTAATTGTAGAATTCAGCGAGAAAGTATTTGACGTGTCTATTAAAACCCGGGCACGTCAAATGGAGAGGTTCTTGCGCCAACCTGTTACTCTTGATGGCCTCTAGAAGGATGTTTAACTTCTGTGTCACAATGTCATCATCCGAAGCTATTTGACATCTTTTTTTTCTTTTTCCTCGATTATTTTTGTTGCATTTTTACCTCAACTTGATATGATGGTACACCACCATCTAGATGGAAATAAGAGTTTCAGCTTTTTATGTAGATTATAATCTTGTAAGAGTTACTTGGTTTAAGAGTATCTTATTCC

mRNA sequence

CCATGTTTAATCGAGGACTAAACAAATTTCATGAGGTATTTGCAGATTCTATTATCAGTTTTCCTTCTGAATCGGTTAATGCTGGTGTAAATAAAGCTGGTTTCTTGACCGTTTCTCTGATTTTGTATTGAGTATGGTTTATCACTTCAATAATGTGGTTATCAACTGGCTGCAGCTCTCAAGGACTTATGCCGCTACATCAGGGAAACATGAACAAAAGGTGAAGGTGCCTCTTGCTTTGTTTGGAGGGTCTGGAAACTATGCCTCTGCTTTGTATATAGCTGCAATAAAAGCTAATTCTCTAGACAAGGTGGAGTCTGAGCTTTTTAACCTCGTTGAGGCTATTAAGAAAAGTCCTATATTTGCTCAGTTCACAATGGACCTGTCAGTTCCAGCGGAGACTAGAGTTAAAGCTATCAATGAAATTTCTGCTGAAGCAAAATTTTCAGACGTTGTTGAGAACTTTTTGGTTGTGTTGGCTGAGAATGGGAGGCTGAGATATATAGATAGCATAGCAAAGAGATTTTTGGAGTTGACTATGGCTCATAAGGGAGAAGTTAAAGCAGTTGTCACAACTGTTATTATTGATCCCAGCATACTTGGTGGTCTAATTGTAGAATTCAGCGAGAAAGTATTTGACGTGTCTATTAAAACCCGGGCACGTCAAATGGAGAGGTTCTTGCGCCAACCTGTTACTCTTGATGGCCTCTAGAAGGATGTTTAACTTCTGTGTCACAATGTCATCATCCGAAGCTATTTGACATCTTTTTTTTCTTTTTCCTCGATTATTTTTGTTGCATTTTTACCTCAACTTGATATGATGGTACACCACCATCTAGATGGAAATAAGAGTTTCAGCTTTTTATGTAGATTATAATCTTGTAAGAGTTACTTGGTTTAAGAGTATCTTATTCC

Coding sequence (CDS)

ATGGTTTATCACTTCAATAATGTGGTTATCAACTGGCTGCAGCTCTCAAGGACTTATGCCGCTACATCAGGGAAACATGAACAAAAGGTGAAGGTGCCTCTTGCTTTGTTTGGAGGGTCTGGAAACTATGCCTCTGCTTTGTATATAGCTGCAATAAAAGCTAATTCTCTAGACAAGGTGGAGTCTGAGCTTTTTAACCTCGTTGAGGCTATTAAGAAAAGTCCTATATTTGCTCAGTTCACAATGGACCTGTCAGTTCCAGCGGAGACTAGAGTTAAAGCTATCAATGAAATTTCTGCTGAAGCAAAATTTTCAGACGTTGTTGAGAACTTTTTGGTTGTGTTGGCTGAGAATGGGAGGCTGAGATATATAGATAGCATAGCAAAGAGATTTTTGGAGTTGACTATGGCTCATAAGGGAGAAGTTAAAGCAGTTGTCACAACTGTTATTATTGATCCCAGCATACTTGGTGGTCTAATTGTAGAATTCAGCGAGAAAGTATTTGACGTGTCTATTAAAACCCGGGCACGTCAAATGGAGAGGTTCTTGCGCCAACCTGTTACTCTTGATGGCCTCTAG

Protein sequence

MVYHFNNVVINWLQLSRTYAATSGKHEQKVKVPLALFGGSGNYASALYIAAIKANSLDKVESELFNLVEAIKKSPIFAQFTMDLSVPAETRVKAINEISAEAKFSDVVENFLVVLAENGRLRYIDSIAKRFLELTMAHKGEVKAVVTTVIIDPSILGGLIVEFSEKVFDVSIKTRARQMERFLRQPVTLDGL

Homology

[Close]

BLAST of Carg07321 vs. NCBI nr
Match: KAG7036582.1 (hypothetical protein SDJN02_00201 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 365.2 bits (936), Expect = 3.6e-97
Identity = 192/192 (100.00%), Postives = 192/192 (100.00%), Query Frame = 0


Query: 1   MVYHFNNVVINWLQLSRTYAATSGKHEQKVKVPLALFGGSGNYASALYIAAIKANSLDKV 60
           MVYHFNNVVINWLQLSRTYAATSGKHEQKVKVPLALFGGSGNYASALYIAAIKANSLDKV
Sbjct: 1   MVYHFNNVVINWLQLSRTYAATSGKHEQKVKVPLALFGGSGNYASALYIAAIKANSLDKV 60

Query: 61  ESELFNLVEAIKKSPIFAQFTMDLSVPAETRVKAINEISAEAKFSDVVENFLVVLAENGR 120
           ESELFNLVEAIKKSPIFAQFTMDLSVPAETRVKAINEISAEAKFSDVVENFLVVLAENGR
Sbjct: 61  ESELFNLVEAIKKSPIFAQFTMDLSVPAETRVKAINEISAEAKFSDVVENFLVVLAENGR 120

Query: 121 LRYIDSIAKRFLELTMAHKGEVKAVVTTVIIDPSILGGLIVEFSEKVFDVSIKTRARQME 180
           LRYIDSIAKRFLELTMAHKGEVKAVVTTVIIDPSILGGLIVEFSEKVFDVSIKTRARQME
Sbjct: 121 LRYIDSIAKRFLELTMAHKGEVKAVVTTVIIDPSILGGLIVEFSEKVFDVSIKTRARQME 180

Query: 181 RFLRQPVTLDGL 193
           RFLRQPVTLDGL
Sbjct: 181 RFLRQPVTLDGL 192

[Close]

BLAST of Carg07321 vs. NCBI nr
Match: XP_022949435.1 (ATP synthase subunit O, mitochondrial-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 318.2 bits (814), Expect = 5.0e-83
Identity = 177/207 (85.51%), Postives = 178/207 (85.99%), Query Frame = 0


Query: 15  LSRTYAATSGKHEQKVKVPLALFGGSGNYASALYIAAIKANSLDKVESELFNLVEAIKKS 74
           LSRTYAATSGKHEQKVKVPLALFGGSGNYASALYIAAIKANSLDKVESELFNLVEAIKKS
Sbjct: 29  LSRTYAATSGKHEQKVKVPLALFGGSGNYASALYIAAIKANSLDKVESELFNLVEAIKKS 88

Query: 75  PIFAQFTMDLSVPAETRVKAINEISAEAKFSDVVENFLVVLAENGRLRYIDSIAKRFLEL 134
           PIFAQFTMDLSVPAETRVKAINEISAEAKFSDVV+NFLVVLAENGRLRYIDSIAKRFLEL
Sbjct: 89  PIFAQFTMDLSVPAETRVKAINEISAEAKFSDVVKNFLVVLAENGRLRYIDSIAKRFLEL 148

Query: 135 TMAHKGEVKAVVTTVI-----------------------------IDPSILGGLIVEFSE 193
           TMAHKGEVKAVVTTVI                             IDPSILGGLIVEFSE
Sbjct: 149 TMAHKGEVKAVVTTVIPIPPQEENELKETLQDIIGQGKKVKLEQKIDPSILGGLIVEFSE 208

[Close]

BLAST of Carg07321 vs. NCBI nr
Match: XP_022949436.1 (ATP synthase subunit O, mitochondrial-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 318.2 bits (814), Expect = 5.0e-83
Identity = 177/207 (85.51%), Postives = 178/207 (85.99%), Query Frame = 0


Query: 15  LSRTYAATSGKHEQKVKVPLALFGGSGNYASALYIAAIKANSLDKVESELFNLVEAIKKS 74
           LSRTYAATSGKHEQKVKVPLALFGGSGNYASALYIAAIKANSLDKVESELFNLVEAIKKS
Sbjct: 27  LSRTYAATSGKHEQKVKVPLALFGGSGNYASALYIAAIKANSLDKVESELFNLVEAIKKS 86

Query: 75  PIFAQFTMDLSVPAETRVKAINEISAEAKFSDVVENFLVVLAENGRLRYIDSIAKRFLEL 134
           PIFAQFTMDLSVPAETRVKAINEISAEAKFSDVV+NFLVVLAENGRLRYIDSIAKRFLEL
Sbjct: 87  PIFAQFTMDLSVPAETRVKAINEISAEAKFSDVVKNFLVVLAENGRLRYIDSIAKRFLEL 146

Query: 135 TMAHKGEVKAVVTTVI-----------------------------IDPSILGGLIVEFSE 193
           TMAHKGEVKAVVTTVI                             IDPSILGGLIVEFSE
Sbjct: 147 TMAHKGEVKAVVTTVIPIPPQEENELKETLQDIIGQGKKVKLEQKIDPSILGGLIVEFSE 206

[Close]

BLAST of Carg07321 vs. NCBI nr
Match: XP_023525828.1 (ATP synthase subunit O, mitochondrial-like isoform X3 [Cucurbita pepo subsp. pepo] >XP_023525830.1 ATP synthase subunit O, mitochondrial-like isoform X4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 304.3 bits (778), Expect = 7.5e-79
Identity = 167/207 (80.68%), Postives = 175/207 (84.54%), Query Frame = 0


Query: 15  LSRTYAATSGKHEQKVKVPLALFGGSGNYASALYIAAIKANSLDKVESELFNLVEAIKKS 74
           LSRTYAATSGKHEQKVK+PLALFGGSGNYASALYIAA+KANSLDKVESELFNLVEAIKKS
Sbjct: 27  LSRTYAATSGKHEQKVKMPLALFGGSGNYASALYIAAVKANSLDKVESELFNLVEAIKKS 86

Query: 75  PIFAQFTMDLSVPAETRVKAINEISAEAKFSDVVENFLVVLAENGRLRYIDSIAKRFLEL 134
           P FAQFTMDLSVPAETRVKAI+EISAEAKFS+VV+NFLVVLAENGRLRYIDSIAKRFLEL
Sbjct: 87  PTFAQFTMDLSVPAETRVKAIDEISAEAKFSEVVKNFLVVLAENGRLRYIDSIAKRFLEL 146

Query: 135 TMAHKGEVKAVVTTVI-----------------------------IDPSILGGLIVEFSE 193
           TMAHKGEVKA VTTVI                             IDPSILGGLIVEF E
Sbjct: 147 TMAHKGEVKAAVTTVIPIPPQEENELKETLQDIIGQGKKVKLEQKIDPSILGGLIVEFDE 206

[Close]

BLAST of Carg07321 vs. NCBI nr
Match: XP_023525826.1 (ATP synthase subunit O, mitochondrial-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023525827.1 ATP synthase subunit O, mitochondrial-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 304.3 bits (778), Expect = 7.5e-79
Identity = 167/207 (80.68%), Postives = 175/207 (84.54%), Query Frame = 0


Query: 15  LSRTYAATSGKHEQKVKVPLALFGGSGNYASALYIAAIKANSLDKVESELFNLVEAIKKS 74
           LSRTYAATSGKHEQKVK+PLALFGGSGNYASALYIAA+KANSLDKVESELFNLVEAIKKS
Sbjct: 29  LSRTYAATSGKHEQKVKMPLALFGGSGNYASALYIAAVKANSLDKVESELFNLVEAIKKS 88

Query: 75  PIFAQFTMDLSVPAETRVKAINEISAEAKFSDVVENFLVVLAENGRLRYIDSIAKRFLEL 134
           P FAQFTMDLSVPAETRVKAI+EISAEAKFS+VV+NFLVVLAENGRLRYIDSIAKRFLEL
Sbjct: 89  PTFAQFTMDLSVPAETRVKAIDEISAEAKFSEVVKNFLVVLAENGRLRYIDSIAKRFLEL 148

Query: 135 TMAHKGEVKAVVTTVI-----------------------------IDPSILGGLIVEFSE 193
           TMAHKGEVKA VTTVI                             IDPSILGGLIVEF E
Sbjct: 149 TMAHKGEVKAAVTTVIPIPPQEENELKETLQDIIGQGKKVKLEQKIDPSILGGLIVEFDE 208

[Close]

BLAST of Carg07321 vs. ExPASy Swiss-Prot
Match: P22778 (ATP synthase subunit O, mitochondrial OS=Ipomoea batatas OX=4120 PE=1 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 4.0e-59
Identity = 121/203 (59.61%), Postives = 148/203 (72.91%), Query Frame = 0


Query: 14  QLSRTYAATSGKHEQKVKVPLALFGGSGNYASALYIAAIKANSLDKVESELFNLVEAIKK 73
           +L R YA  S   EQK+KVPL ++G SGNYASALY+AA+K+N+L+KVESEL++LVEA KK
Sbjct: 40  ELLRNYATASASKEQKIKVPLTMYGVSGNYASALYLAAVKSNTLEKVESELYDLVEASKK 99

Query: 74  SPIFAQFTMDLSVPAETRVKAINEISAEAKFSDVVENFLVVLAENGRLRYIDSIAKRFLE 133
           SP F+QF  D SVP +TRV AI EI A+AKF D  +NFL++LAENGRL++ID I KRF E
Sbjct: 100 SPTFSQFMRDPSVPVDTRVNAIKEICAQAKFGDTTQNFLLILAENGRLKHIDRIVKRFKE 159

Query: 134 LTMAHKGEVKAVVTTVI-----------------------------IDPSILGGLIVEFS 188
           LTMAH+GEVKA VTTVI                             IDP+ILGGL+VEF 
Sbjct: 160 LTMAHRGEVKATVTTVIPLPADEEKELKATLQEMVGQGKSVQIEQKIDPTILGGLVVEFG 219

[Close]

BLAST of Carg07321 vs. ExPASy Swiss-Prot
Match: Q96251 (ATP synthase subunit O, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g13450 PE=1 SV=2)

HSP 1 Score: 191.4 bits (485), Expect = 9.3e-48
Identity = 103/205 (50.24%), Postives = 139/205 (67.80%), Query Frame = 0


Query: 17  RTYAATSGKHEQKVKVPLALFGGSGNYASALYIAAIKANSLDKVESELFNLVEAIKKSPI 76
           RTYA  S +    VKVP+AL G +GN+AS LYIAA+K NSL+K+E++L  ++EA+K +PI
Sbjct: 34  RTYATASAQTTANVKVPIALVGENGNFASWLYIAAVKMNSLEKIETDLSEMIEAMKTAPI 93

Query: 77  FAQFTMDLSVPAETRVKAINEISAEAKFSDVVENFLVVLAENGRLRYIDSIAKRFLELTM 136
           FAQFT D SVP  TR+ AI +   +AKF++  +NFL +LAENG+L+ +D+I K+F++LT 
Sbjct: 94  FAQFTKDPSVPRGTRLAAIRDACDQAKFAEPTKNFLSLLAENGKLKNLDAIVKKFMQLTN 153

Query: 137 AHKGEVKAVVTTVI-----------------------------IDPSILGGLIVEFSEKV 193
           AH+G+VK +VTTVI                             IDPSI GGLIVEF +KV
Sbjct: 154 AHRGDVKVLVTTVIPLPPAEEKELTETLQEIIGAGKKITVEQKIDPSIYGGLIVEFQQKV 213

[Close]

BLAST of Carg07321 vs. ExPASy Swiss-Prot
Match: Q5RD23 (ATP synthase subunit O, mitochondrial OS=Pongo abelii OX=9601 GN=ATP5PO PE=2 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 7.4e-21
Identity = 65/185 (35.14%), Postives = 99/185 (53.51%), Query Frame = 0


Query: 30  VKVPLALFGGSGNYASALYIAAIKANSLDKVESELFNLVEAIKKSPIFAQFTMDLSVPAE 89
           V+ P+ ++G  G YA+ALY AA K N L++VE EL  + + I K P  A   ++  V   
Sbjct: 28  VRPPVQVYGIEGRYATALYSAASKQNKLEQVEKELLRVAQ-ILKEPKVAAPVLNPYVKRS 87

Query: 90  TRVKAINEISAEAKFSDVVENFLVVLAENGRLRYIDSIAKRFLELTMAHKGEVKAVVT-- 149
            +VK++N+I+A+ +FS +  N + +LAENGRL     +   F  +  AH+GEV   VT  
Sbjct: 88  IKVKSLNDITAKERFSPLTTNLINLLAENGRLSNTQGVVSAFSTMMSAHRGEVPCTVTSA 147

Query: 150 ------------TVI---------------IDPSILGGLIVEFSEKVFDVSIKTRARQME 186
                       TV+                DPSILGG+IV   EK  D+S+KT+ +++ 
Sbjct: 148 SPLEEATLSELKTVLKSFLSQGQVLKLEAKTDPSILGGMIVRIGEKYVDMSVKTKIQKLS 207

[Close]

BLAST of Carg07321 vs. ExPASy Swiss-Prot
Match: P13621 (ATP synthase subunit O, mitochondrial OS=Bos taurus OX=9913 GN=ATP5PO PE=1 SV=2)

HSP 1 Score: 101.7 bits (252), Expect = 9.6e-21
Identity = 64/185 (34.59%), Postives = 97/185 (52.43%), Query Frame = 0


Query: 30  VKVPLALFGGSGNYASALYIAAIKANSLDKVESELFNLVEAIKKSPIFAQFTMDLSVPAE 89
           V+ P+ ++G  G YA+ALY AA K N L++VE EL   V  I K P  A   ++  V   
Sbjct: 28  VRPPVQIYGIEGRYATALYSAASKQNKLEQVEKELLR-VGQILKEPKMAASLLNPYVKRS 87

Query: 90  TRVKAINEISAEAKFSDVVENFLVVLAENGRLRYIDSIAKRFLELTMAHKGEVKAVVTT- 149
            +VK++++++A+ KFS +  N + +LAENGRL    ++   F  +   H+GEV   VTT 
Sbjct: 88  VKVKSLSDMTAKEKFSPLTSNLINLLAENGRLTNTPAVISAFSTMMSVHRGEVPCTVTTA 147

Query: 150 ----------------------------VIIDPSILGGLIVEFSEKVFDVSIKTRARQME 186
                                       V IDPSI+GG+IV   EK  D+S KT+ +++ 
Sbjct: 148 SALDEATLTELKTVLKSFLSKGQVLKLEVKIDPSIMGGMIVRIGEKYVDMSAKTKIQKLS 207

[Close]

BLAST of Carg07321 vs. ExPASy Swiss-Prot
Match: P48047 (ATP synthase subunit O, mitochondrial OS=Homo sapiens OX=9606 GN=ATP5PO PE=1 SV=1)

HSP 1 Score: 101.3 bits (251), Expect = 1.3e-20
Identity = 65/187 (34.76%), Postives = 99/187 (52.94%), Query Frame = 0


Query: 30  VKVPLALFGGSGNYASALYIAAIKANSLDKVESELFNLVEAIKKSPIFAQFTMDLSVPAE 89
           V+ P+ ++G  G YA+ALY AA K N L++VE EL  + + I K P  A   ++  V   
Sbjct: 28  VRPPVQVYGIEGRYATALYSAASKQNKLEQVEKELLRVAQ-ILKEPKVAASVLNPYVKRS 87

Query: 90  TRVKAINEISAEAKFSDVVENFLVVLAENGRLRYIDSIAKRFLELTMAHKGEVKAVVT-- 149
            +VK++N+I+A+ +FS +  N + +LAENGRL     +   F  +   H+GEV   VT  
Sbjct: 88  IKVKSLNDITAKERFSPLTTNLINLLAENGRLSNTQGVVSAFSTMMSVHRGEVPCTVTSA 147

Query: 150 ------------TVI---------------IDPSILGGLIVEFSEKVFDVSIKTRARQME 188
                       TV+                DPSILGG+IV   EK  D+S+KT+ +++ 
Sbjct: 148 SPLEEATLSELKTVLKSFLSQGQVLKLEAKTDPSILGGMIVRIGEKYVDMSVKTKIQKLG 207

[Close]

BLAST of Carg07321 vs. ExPASy TrEMBL
Match: A0A6J1GCR6 (ATP synthase subunit O, mitochondrial-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111452782 PE=3 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 2.4e-83
Identity = 177/207 (85.51%), Postives = 178/207 (85.99%), Query Frame = 0


Query: 15  LSRTYAATSGKHEQKVKVPLALFGGSGNYASALYIAAIKANSLDKVESELFNLVEAIKKS 74
           LSRTYAATSGKHEQKVKVPLALFGGSGNYASALYIAAIKANSLDKVESELFNLVEAIKKS
Sbjct: 29  LSRTYAATSGKHEQKVKVPLALFGGSGNYASALYIAAIKANSLDKVESELFNLVEAIKKS 88

Query: 75  PIFAQFTMDLSVPAETRVKAINEISAEAKFSDVVENFLVVLAENGRLRYIDSIAKRFLEL 134
           PIFAQFTMDLSVPAETRVKAINEISAEAKFSDVV+NFLVVLAENGRLRYIDSIAKRFLEL
Sbjct: 89  PIFAQFTMDLSVPAETRVKAINEISAEAKFSDVVKNFLVVLAENGRLRYIDSIAKRFLEL 148

Query: 135 TMAHKGEVKAVVTTVI-----------------------------IDPSILGGLIVEFSE 193
           TMAHKGEVKAVVTTVI                             IDPSILGGLIVEFSE
Sbjct: 149 TMAHKGEVKAVVTTVIPIPPQEENELKETLQDIIGQGKKVKLEQKIDPSILGGLIVEFSE 208

[Close]

BLAST of Carg07321 vs. ExPASy TrEMBL
Match: A0A6J1GC08 (ATP synthase subunit O, mitochondrial-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111452782 PE=3 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 2.4e-83
Identity = 177/207 (85.51%), Postives = 178/207 (85.99%), Query Frame = 0


Query: 15  LSRTYAATSGKHEQKVKVPLALFGGSGNYASALYIAAIKANSLDKVESELFNLVEAIKKS 74
           LSRTYAATSGKHEQKVKVPLALFGGSGNYASALYIAAIKANSLDKVESELFNLVEAIKKS
Sbjct: 27  LSRTYAATSGKHEQKVKVPLALFGGSGNYASALYIAAIKANSLDKVESELFNLVEAIKKS 86

Query: 75  PIFAQFTMDLSVPAETRVKAINEISAEAKFSDVVENFLVVLAENGRLRYIDSIAKRFLEL 134
           PIFAQFTMDLSVPAETRVKAINEISAEAKFSDVV+NFLVVLAENGRLRYIDSIAKRFLEL
Sbjct: 87  PIFAQFTMDLSVPAETRVKAINEISAEAKFSDVVKNFLVVLAENGRLRYIDSIAKRFLEL 146

Query: 135 TMAHKGEVKAVVTTVI-----------------------------IDPSILGGLIVEFSE 193
           TMAHKGEVKAVVTTVI                             IDPSILGGLIVEFSE
Sbjct: 147 TMAHKGEVKAVVTTVIPIPPQEENELKETLQDIIGQGKKVKLEQKIDPSILGGLIVEFSE 206

[Close]

BLAST of Carg07321 vs. ExPASy TrEMBL
Match: A0A6J1KAB8 (ATP synthase subunit O, mitochondrial-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111493106 PE=3 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 5.8e-77
Identity = 164/204 (80.39%), Postives = 172/204 (84.31%), Query Frame = 0


Query: 15  LSRTYAATSGKHEQKVKVPLALFGGSGNYASALYIAAIKANSLDKVESELFNLVEAIKKS 74
           L+RTYAATSGKHE+KVKVPLALFGGSGNYASALYIAA+KANSLDKVESELFNLVEAIKKS
Sbjct: 29  LARTYAATSGKHERKVKVPLALFGGSGNYASALYIAAVKANSLDKVESELFNLVEAIKKS 88

Query: 75  PIFAQFTMDLSVPAETRVKAINEISAEAKFSDVVENFLVVLAENGRLRYIDSIAKRFLEL 134
           P F+QFTMDLSVPAETRVKAINEISAEAKFSDVV+NFLVVLAENGRLRYIDSIAKRFLEL
Sbjct: 89  PTFSQFTMDLSVPAETRVKAINEISAEAKFSDVVKNFLVVLAENGRLRYIDSIAKRFLEL 148

Query: 135 TMAHKGEVKAVVTTVI-----------------------------IDPSILGGLIVEFSE 190
           TMAHKGEVKAVVTTVI                             IDPSILGGLIVEF E
Sbjct: 149 TMAHKGEVKAVVTTVIPIPPQEENELKETLQDIIGQGKKVKLEQKIDPSILGGLIVEFDE 208

[Close]

BLAST of Carg07321 vs. ExPASy TrEMBL
Match: A0A6J1K855 (ATP synthase subunit O, mitochondrial-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111493106 PE=3 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 5.8e-77
Identity = 164/204 (80.39%), Postives = 172/204 (84.31%), Query Frame = 0


Query: 15  LSRTYAATSGKHEQKVKVPLALFGGSGNYASALYIAAIKANSLDKVESELFNLVEAIKKS 74
           L+RTYAATSGKHE+KVKVPLALFGGSGNYASALYIAA+KANSLDKVESELFNLVEAIKKS
Sbjct: 27  LARTYAATSGKHERKVKVPLALFGGSGNYASALYIAAVKANSLDKVESELFNLVEAIKKS 86

Query: 75  PIFAQFTMDLSVPAETRVKAINEISAEAKFSDVVENFLVVLAENGRLRYIDSIAKRFLEL 134
           P F+QFTMDLSVPAETRVKAINEISAEAKFSDVV+NFLVVLAENGRLRYIDSIAKRFLEL
Sbjct: 87  PTFSQFTMDLSVPAETRVKAINEISAEAKFSDVVKNFLVVLAENGRLRYIDSIAKRFLEL 146

Query: 135 TMAHKGEVKAVVTTVI-----------------------------IDPSILGGLIVEFSE 190
           TMAHKGEVKAVVTTVI                             IDPSILGGLIVEF E
Sbjct: 147 TMAHKGEVKAVVTTVIPIPPQEENELKETLQDIIGQGKKVKLEQKIDPSILGGLIVEFDE 206

[Close]

BLAST of Carg07321 vs. ExPASy TrEMBL
Match: A0A6J1DGN4 (ATP synthase subunit O, mitochondrial-like OS=Momordica charantia OX=3673 GN=LOC111020274 PE=3 SV=1)

HSP 1 Score: 293.5 bits (750), Expect = 6.4e-76
Identity = 163/209 (77.99%), Postives = 174/209 (83.25%), Query Frame = 0


Query: 13  LQLSRTYAATSGKHEQKVKVPLALFGGSGNYASALYIAAIKANSLDKVESELFNLVEAIK 72
           + LSRTY+ATSGK E KVKVPLALFGGSGNYASALY+AA+KANSL+KVE+EL NLVEAIK
Sbjct: 27  IPLSRTYSATSGKVE-KVKVPLALFGGSGNYASALYLAAVKANSLEKVEAELVNLVEAIK 86

Query: 73  KSPIFAQFTMDLSVPAETRVKAINEISAEAKFSDVVENFLVVLAENGRLRYIDSIAKRFL 132
           KSP F+QFTMDLSVPAETRVKAINEISAEAKFSDVV+NFLVVLAENGRLRY+DSIAKRFL
Sbjct: 87  KSPTFSQFTMDLSVPAETRVKAINEISAEAKFSDVVKNFLVVLAENGRLRYVDSIAKRFL 146

Query: 133 ELTMAHKGEVKAVVTTVI-----------------------------IDPSILGGLIVEF 192
           ELTMAHKGEVKAVVTTVI                             IDPSILGGL+VEF
Sbjct: 147 ELTMAHKGEVKAVVTTVIPIPPQEEKELKETLQDIIGQGKNVKLEQKIDPSILGGLVVEF 206

[Close]

BLAST of Carg07321 vs. TAIR 10
Match: AT5G13450.1 (delta subunit of Mt ATP synthase )

HSP 1 Score: 191.4 bits (485), Expect = 6.6e-49
Identity = 103/205 (50.24%), Postives = 139/205 (67.80%), Query Frame = 0


Query: 17  RTYAATSGKHEQKVKVPLALFGGSGNYASALYIAAIKANSLDKVESELFNLVEAIKKSPI 76
           RTYA  S +    VKVP+AL G +GN+AS LYIAA+K NSL+K+E++L  ++EA+K +PI
Sbjct: 34  RTYATASAQTTANVKVPIALVGENGNFASWLYIAAVKMNSLEKIETDLSEMIEAMKTAPI 93

Query: 77  FAQFTMDLSVPAETRVKAINEISAEAKFSDVVENFLVVLAENGRLRYIDSIAKRFLELTM 136
           FAQFT D SVP  TR+ AI +   +AKF++  +NFL +LAENG+L+ +D+I K+F++LT 
Sbjct: 94  FAQFTKDPSVPRGTRLAAIRDACDQAKFAEPTKNFLSLLAENGKLKNLDAIVKKFMQLTN 153

Query: 137 AHKGEVKAVVTTVI-----------------------------IDPSILGGLIVEFSEKV 193
           AH+G+VK +VTTVI                             IDPSI GGLIVEF +KV
Sbjct: 154 AHRGDVKVLVTTVIPLPPAEEKELTETLQEIIGAGKKITVEQKIDPSIYGGLIVEFQQKV 213

[Close]

BLAST of Carg07321 vs. TAIR 10
Match: AT5G13450.2 (delta subunit of Mt ATP synthase )

HSP 1 Score: 121.3 bits (303), Expect = 8.4e-28
Identity = 67/143 (46.85%), Postives = 91/143 (63.64%), Query Frame = 0


Query: 79  QFTMDLSVPAETRVKAINEISAEAKFSDVVENFLVVLAENGRLRYIDSIAKRFLELTMAH 138
           +FT D SVP  TR+ AI +   +AKF++  +NFL +LAENG+L+ +D+I K+F++LT AH
Sbjct: 48  KFTKDPSVPRGTRLAAIRDACDQAKFAEPTKNFLSLLAENGKLKNLDAIVKKFMQLTNAH 107

Query: 139 KGEVKAVVTTVI-----------------------------IDPSILGGLIVEFSEKVFD 193
           +G+VK +VTTVI                             IDPSI GGLIVEF +KV D
Sbjct: 108 RGDVKVLVTTVIPLPPAEEKELTETLQEIIGAGKKITVEQKIDPSIYGGLIVEFQQKVLD 167

The following BLAST results are available for this feature:

BLAST of Carg07321 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7036582.1	3.6e-97	100.00	hypothetical protein SDJN02_00201 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022949435.1	5.0e-83	85.51	ATP synthase subunit O, mitochondrial-like isoform X1 [Cucurbita moschata]	[more]
XP_022949436.1	5.0e-83	85.51	ATP synthase subunit O, mitochondrial-like isoform X2 [Cucurbita moschata]	[more]
XP_023525828.1	7.5e-79	80.68	ATP synthase subunit O, mitochondrial-like isoform X3 [Cucurbita pepo subsp. pep...	[more]
XP_023525826.1	7.5e-79	80.68	ATP synthase subunit O, mitochondrial-like isoform X1 [Cucurbita pepo subsp. pep...	[more]

BLAST of Carg07321 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P22778	4.0e-59	59.61	ATP synthase subunit O, mitochondrial OS=Ipomoea batatas OX=4120 PE=1 SV=1	[more]
Q96251	9.3e-48	50.24	ATP synthase subunit O, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g134...	[more]
Q5RD23	7.4e-21	35.14	ATP synthase subunit O, mitochondrial OS=Pongo abelii OX=9601 GN=ATP5PO PE=2 SV=...	[more]
P13621	9.6e-21	34.59	ATP synthase subunit O, mitochondrial OS=Bos taurus OX=9913 GN=ATP5PO PE=1 SV=2	[more]
P48047	1.3e-20	34.76	ATP synthase subunit O, mitochondrial OS=Homo sapiens OX=9606 GN=ATP5PO PE=1 SV=...	[more]

BLAST of Carg07321 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GCR6	2.4e-83	85.51	ATP synthase subunit O, mitochondrial-like isoform X1 OS=Cucurbita moschata OX=3...	[more]
A0A6J1GC08	2.4e-83	85.51	ATP synthase subunit O, mitochondrial-like isoform X2 OS=Cucurbita moschata OX=3...	[more]
A0A6J1KAB8	5.8e-77	80.39	ATP synthase subunit O, mitochondrial-like isoform X1 OS=Cucurbita maxima OX=366...	[more]
A0A6J1K855	5.8e-77	80.39	ATP synthase subunit O, mitochondrial-like isoform X2 OS=Cucurbita maxima OX=366...	[more]
A0A6J1DGN4	6.4e-76	77.99	ATP synthase subunit O, mitochondrial-like OS=Momordica charantia OX=3673 GN=LOC...	[more]

BLAST of Carg07321 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G13450.1	6.6e-49	50.24	delta subunit of Mt ATP synthase	[more]
AT5G13450.2	8.4e-28	46.85	delta subunit of Mt ATP synthase	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000711	ATPase, OSCP/delta subunit	PRINTS	PR00125	ATPASEDELTA	coord: 120..134 score: 28.17 coord: 39..58 score: 36.75 coord: 109..120 score: 46.25
IPR000711	ATPase, OSCP/delta subunit	PFAM	PF00213	OSCP	coord: 41..149 e-value: 1.9E-23 score: 83.5
IPR000711	ATPase, OSCP/delta subunit	PANTHER	PTHR11910	ATP SYNTHASE DELTA CHAIN	coord: 14..185
IPR026015	F1F0 ATP synthase OSCP/delta subunit, N-terminal domain superfamily	GENE3D	1.10.520.20		coord: 39..132 e-value: 5.0E-20 score: 73.9
IPR026015	F1F0 ATP synthase OSCP/delta subunit, N-terminal domain superfamily	SUPERFAMILY	47928	N-terminal domain of the delta subunit of the F1F0-ATP synthase	coord: 40..141
None	No IPR available	PANTHER	PTHR11910:SF1	ATP SYNTHASE SUBUNIT O, MITOCHONDRIAL	coord: 14..185
IPR020781	ATPase, OSCP/delta subunit, conserved site	PROSITE	PS00389	ATPASE_DELTA	coord: 145..164

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg07321-RA	Carg07321-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015986	ATP synthesis coupled proton transport
cellular_component	GO:0016020	membrane
molecular_function	GO:0046933	proton-transporting ATP synthase activity, rotational mechanism