Carg07307 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg07307
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionProtein kinase domain-containing protein
LocationCarg_Chr01: 1019597 .. 1023099 (-)
RNA-Seq ExpressionCarg07307
SyntenyCarg07307
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGGAGGTTCAAGCCTTTCATGCACAAAGAACCATCTGGTCTCGAGGGTCGCTCGATTGATGTTGGCAATCTTAAGATTCATGTTAGAAACGTCATTGCGGAAGGTGGATTCTCTTGTGTGTACTTAGCCAAGGATGCTCTACATGTTTCGAAACAGTATGCTTTGAAGCACATTATATGCAATGATGTGGAATCTCTTGAATTGGCGTTGAAGGAGATTTCTGTAATGAAATCACTCCGAGGCCATCCGAATGTTGTTACACTTTATGCCCATACGGTCGTAGATATGGGACGCACAAAAGAAGTTCTAATTGTGATGGAATTTTGTGAGAAGTCTTTGGTTAATGTCTTGGAGAGCAGAGGAGCTGAATTCTTTGATGAAACTCAAGTTCTCTTCATTTTCCGAGACGTGTGCAATGCAGTATTCGCAATGCATTGTCGTTCCCCGCCAATTGCTCACAGGTAAACATGGTAGCTAGTTGTCTATATCCCAACAATCTTTCTCTTAAAAGTTATAATAGCTTCCACAATATTCATTCTGTGGTCTTCCATGCCTAGTTAATGAACTAAAGTGATTTTATCTGCTAGTAGTTTAGTTGTTTAACTGATGTTCTGTAGTTAAGATATAAAAGATCTCTATTCAATATCTCTGAAGTCATGAAGTCATGTCTGCTGTGAGATCCCACATCGATTGGAGAGAGCAACAAGTGCCAGCGAGACATCCCACATCGATTGGAGAGAGCAACGAGTGCCAGCGAGGACGTTGGGCCCCTAAGGTGGGTGGATGGTGAAATCCCACATTGGCTAGGGAGGAGAACGAAACATTCTTTATAAGGGTGTGAAAAACTCCCCCTAACAAACACGTTTTAAAAACTGTGAAGGAAAGCCTGAAAGGGAAAGCCCAAATAGGACAATATCTGCTAGCGGTGGGCTTGAGTTGTTACATCTGCTCTATGTTATTTGTGGTACATGGTTTTGGTTGATGTAATCCCAACCATGTTTCGAGGCACTTGTCATTGGTGTATGACTTATGTTCTTTGTTATGGCTTAACTAAGATCTTGATTTTCTCTTGTGATTGCGATTTTCAGAGATTTGAAAGCTGAAAATCTTTTGTTAGGCTCAGATGGCCACTGGAAATTATGTGATTTCGGAAGCACTTCTACGAATCACAAGCGCTTCGAGAAGCCCGAGGAAATGGGCATTGAAGAAGATAACATCAGGAAGCATACTACACCTGCCTATAGAGCCCCTGAGGTATCAAAAAAATCCACATCTTGATCCTAATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCAGTTGTGATATTTGGTTGTCAGAATGTTAGTAATGTTTATCTATTTCCTGTTATGACACAGATGTGGGATCTATTCCGTAGAGAACTTATAAATGAGAAAGTTGACATATGGGTATGTATACTCTTCCTCTTTACCGATGTCGAAAGGTTAAATTGTGATATAGTGTAAAATCCCACCTCGATTGGGGAGGAGAACAAAGCATTCCTTATAAGGGTGTGGTTACAAATGATATCAGAGTTAGACACCGAGCGGGGGTGGATTGTGAGATCCCACGTTGATTGGAGAGGAGAACGAAGCATTCCTTATAAGGGTGTGGGAACTTCTTCCTGATAGACGCGTTTTAAAACCGTGAGGCTGACGGCGAAACGTAACAAGTTAAAGTGGACAATATTTGTTAGCGGTGGACTTGGGCTGTTACATATTAGTTCTCAACAATTTCCTTCACAAATGAACTTATAAAGAATTGATGTTCTTTTCATTTCTGAGATTTGAATTCTATTACACGACAGGCTCTCGGGTGTCTTCTCTTCCGCATATGTTACTTCAAAAGTGCTTTTGATGGAGAATCGAAACTTCAAATTTTAAATGGGAACTATCGAATTCTGGAGGTACCAAAATATAGCTCATCTATTACAGACCTAATCAAAGATATGCTCCAAGCGTCACCAAATGACAGACCAGACATCACGCAGGCAAGCGCTTTGCTTGATTGGCCATTCATCTCCATGAATTTAGGTTAGTTTAGATTACTCATGTCGATGACGATAATAGGTGTCAGGATTCTCATAATTCCTTCCCGATCTTGGGGTTGGTTTGTGCCGTGGCAGGTCTGGTTTCGTGTTAACAACCAGCTACCTGTTCGATCACGAAAGTCATTACCTGATCAGCCACCTGACATGCAGTCTACAGAGAAACAGGAAGGTGAGGGTGAAGGTAGCTTAAGGGTTCATTAATTTGATTAGTTCTATGTTGTCTAGTTCATCAAGAAAGCCAGAGGTCGTTTATATCTTTAGTCTCCAAATTTGTGAAGGGATTAATTATGGTATGGTGTCGAAGTTTGTTGATATAAATGGTATCTTAGCAGGCGCTTTGAACCTTATGAACAAGTCGTCTCCTATGCCTTGTAGAAGTCCGCCCCCACCTCCATCAGTTAAAAGTCCATCACAGGCAGCGCATGCGCCGAAGGCCACAGGCGGAGGGGGAGGGGGAGTTGGAGGAGGACCGCTGGGTGCCTTCTGGTCTACTCAACACGCCAATGAAACGACTAATAAAGATACAAGCCAAATTAGGTTCGACGAAGAATCAACCTCTCGTAGTACCTCGAAACACGAGAGGATTAATCCAAACAATCATTCTGCAGTTAAGAATGCTAGTCCTGGGGATAGCAACCAAAAGTCAAATAAGACAGTGAGTGATAGTGGTTCATTCAGAGACTTTGAATTATCGTTTTTCCAGAACGAAACGGGACGTAGCTCTAATGGAGCGAAAGCATCAAAACCCGAGAATGCAGCCTTCCAAGACAAAGCTTTTAACAATTTTGTTGCTGAATTTGATACCTCTAAGTTCAGTTCAGATGTCACTAACAACAGACCAGGAAAGGAAGTAGCAGCATCAGAGGCTGAGGTGGAGAAGTTGAAGGAGCAACTGAAGCAAGCAAACATGGAGAAAGCTGAAATCACATCCAAATTTGAAAAGCTGTCTGCCATCTGCCGGTCTCAAAGGCAAGAGATACAGGAGCTCAAGCAATCACTTGCTGCTAGGAACATTCAGCCTTCTGCTGTACCTCGAGTATTTCCTCGCTTCCTTTTTCGTCCTTTCGATTTGGTTTTCCAAGGAAAACCGCCATCTTTATGCATCTTTTACCTCACATTTCAGGTACAGAAGGCCGATTCGAATACTCGAACCCCTGATGCAAAGCCATGGCAGGCCTTTGCAGGAGAATCCCCAATCCAGCAGCCTGATACGCCTGAAAAGGGTGTCAAATCTGTGAGGACTAGAAGCCTGCCGAGAAAGCAGGCTTCTACAGAAGGCACTGGTTTCGAAAGCTGGGGCTTTGGATCCGAGAGTTTTACAGCCGTGTCTGCTGGAAGCTCCAACGCATCCAGATTAACTGGTGAAAGGTCTAGTTCACAAAGAACTGACAGAGCTTCACAACCTGCTGGATGGGCGGGTTTCTAG

mRNA sequence

ATGTGGAGGTTCAAGCCTTTCATGCACAAAGAACCATCTGGTCTCGAGGGTCGCTCGATTGATGTTGGCAATCTTAAGATTCATGTTAGAAACGTCATTGCGGAAGGTGGATTCTCTTGTGTGTACTTAGCCAAGGATGCTCTACATGTTTCGAAACAGTATGCTTTGAAGCACATTATATGCAATGATGTGGAATCTCTTGAATTGGCGTTGAAGGAGATTTCTGTAATGAAATCACTCCGAGGCCATCCGAATGTTGTTACACTTTATGCCCATACGGTCGTAGATATGGGACGCACAAAAGAAGTTCTAATTGTGATGGAATTTTGTGAGAAGTCTTTGGTTAATGTCTTGGAGAGCAGAGGAGCTGAATTCTTTGATGAAACTCAAGTTCTCTTCATTTTCCGAGACGTGTGCAATGCAGTATTCGCAATGCATTGTCGTTCCCCGCCAATTGCTCACAGAGATTTGAAAGCTGAAAATCTTTTGTTAGGCTCAGATGGCCACTGGAAATTATGTGATTTCGGAAGCACTTCTACGAATCACAAGCGCTTCGAGAAGCCCGAGGAAATGGGCATTGAAGAAGATAACATCAGGAAGCATACTACACCTGCCTATAGAGCCCCTGAGGTCTGGTTTCGTGTTAACAACCAGCTACCTGTTCGATCACGAAAGTCATTACCTGATCAGCCACCTGACATGCAGTCTACAGAGAAACAGGAAGGTGAGGGTGAAGGCGCTTTGAACCTTATGAACAAGTCGTCTCCTATGCCTTGTAGAAGTCCGCCCCCACCTCCATCAGTTAAAAGTCCATCACAGGCAGCGCATGCGCCGAAGGCCACAGGCGGAGGGGGAGGGGGAGTTGGAGGAGGACCGCTGGGTGCCTTCTGGTCTACTCAACACGCCAATGAAACGACTAATAAAGATACAAGCCAAATTAGGTTCGACGAAGAATCAACCTCTCGTAGTACCTCGAAACACGAGAGGATTAATCCAAACAATCATTCTGCAGTTAAGAATGCTAGTCCTGGGGATAGCAACCAAAAGTCAAATAAGACAGTGAGTGATAGTGGTTCATTCAGAGACTTTGAATTATCGTTTTTCCAGAACGAAACGGGACGTAGCTCTAATGGAGCGAAAGCATCAAAACCCGAGAATGCAGCCTTCCAAGACAAAGCTTTTAACAATTTTGTTGCTGAATTTGATACCTCTAAGTTCAGTTCAGATGTCACTAACAACAGACCAGGAAAGGAAGTAGCAGCATCAGAGGCTGAGGTGGAGAAGTTGAAGGAGCAACTGAAGCAAGCAAACATGGAGAAAGCTGAAATCACATCCAAATTTGAAAAGCTGTCTGCCATCTGCCGGTCTCAAAGGCAAGAGATACAGGAGCTCAAGCAATCACTTGCTGCTAGGAACATTCAGCCTTCTGCTGTACCTCGAGTATTTCCTCGCTTCCTTTTTCGTCCTTTCGATTTGGTTTTCCAAGGAAAACCGCCATCTTTATGCATCTTTTACCTCACATTTCAGGTACAGAAGGCCGATTCGAATACTCGAACCCCTGATGCAAAGCCATGGCAGGCCTTTGCAGGAGAATCCCCAATCCAGCAGCCTGATACGCCTGAAAAGGGTGTCAAATCTGTGAGGACTAGAAGCCTGCCGAGAAAGCAGGCTTCTACAGAAGGCACTGGTTTCGAAAGCTGGGGCTTTGGATCCGAGAGTTTTACAGCCGTGTCTGCTGGAAGCTCCAACGCATCCAGATTAACTGGTGAAAGGTCTAGTTCACAAAGAACTGACAGAGCTTCACAACCTGCTGGATGGGCGGGTTTCTAG

Coding sequence (CDS)

ATGTGGAGGTTCAAGCCTTTCATGCACAAAGAACCATCTGGTCTCGAGGGTCGCTCGATTGATGTTGGCAATCTTAAGATTCATGTTAGAAACGTCATTGCGGAAGGTGGATTCTCTTGTGTGTACTTAGCCAAGGATGCTCTACATGTTTCGAAACAGTATGCTTTGAAGCACATTATATGCAATGATGTGGAATCTCTTGAATTGGCGTTGAAGGAGATTTCTGTAATGAAATCACTCCGAGGCCATCCGAATGTTGTTACACTTTATGCCCATACGGTCGTAGATATGGGACGCACAAAAGAAGTTCTAATTGTGATGGAATTTTGTGAGAAGTCTTTGGTTAATGTCTTGGAGAGCAGAGGAGCTGAATTCTTTGATGAAACTCAAGTTCTCTTCATTTTCCGAGACGTGTGCAATGCAGTATTCGCAATGCATTGTCGTTCCCCGCCAATTGCTCACAGAGATTTGAAAGCTGAAAATCTTTTGTTAGGCTCAGATGGCCACTGGAAATTATGTGATTTCGGAAGCACTTCTACGAATCACAAGCGCTTCGAGAAGCCCGAGGAAATGGGCATTGAAGAAGATAACATCAGGAAGCATACTACACCTGCCTATAGAGCCCCTGAGGTCTGGTTTCGTGTTAACAACCAGCTACCTGTTCGATCACGAAAGTCATTACCTGATCAGCCACCTGACATGCAGTCTACAGAGAAACAGGAAGGTGAGGGTGAAGGCGCTTTGAACCTTATGAACAAGTCGTCTCCTATGCCTTGTAGAAGTCCGCCCCCACCTCCATCAGTTAAAAGTCCATCACAGGCAGCGCATGCGCCGAAGGCCACAGGCGGAGGGGGAGGGGGAGTTGGAGGAGGACCGCTGGGTGCCTTCTGGTCTACTCAACACGCCAATGAAACGACTAATAAAGATACAAGCCAAATTAGGTTCGACGAAGAATCAACCTCTCGTAGTACCTCGAAACACGAGAGGATTAATCCAAACAATCATTCTGCAGTTAAGAATGCTAGTCCTGGGGATAGCAACCAAAAGTCAAATAAGACAGTGAGTGATAGTGGTTCATTCAGAGACTTTGAATTATCGTTTTTCCAGAACGAAACGGGACGTAGCTCTAATGGAGCGAAAGCATCAAAACCCGAGAATGCAGCCTTCCAAGACAAAGCTTTTAACAATTTTGTTGCTGAATTTGATACCTCTAAGTTCAGTTCAGATGTCACTAACAACAGACCAGGAAAGGAAGTAGCAGCATCAGAGGCTGAGGTGGAGAAGTTGAAGGAGCAACTGAAGCAAGCAAACATGGAGAAAGCTGAAATCACATCCAAATTTGAAAAGCTGTCTGCCATCTGCCGGTCTCAAAGGCAAGAGATACAGGAGCTCAAGCAATCACTTGCTGCTAGGAACATTCAGCCTTCTGCTGTACCTCGAGTATTTCCTCGCTTCCTTTTTCGTCCTTTCGATTTGGTTTTCCAAGGAAAACCGCCATCTTTATGCATCTTTTACCTCACATTTCAGGTACAGAAGGCCGATTCGAATACTCGAACCCCTGATGCAAAGCCATGGCAGGCCTTTGCAGGAGAATCCCCAATCCAGCAGCCTGATACGCCTGAAAAGGGTGTCAAATCTGTGAGGACTAGAAGCCTGCCGAGAAAGCAGGCTTCTACAGAAGGCACTGGTTTCGAAAGCTGGGGCTTTGGATCCGAGAGTTTTACAGCCGTGTCTGCTGGAAGCTCCAACGCATCCAGATTAACTGGTGAAAGGTCTAGTTCACAAAGAACTGACAGAGCTTCACAACCTGCTGGATGGGCGGGTTTCTAG

Protein sequence

MWRFKPFMHKEPSGLEGRSIDVGNLKIHVRNVIAEGGFSCVYLAKDALHVSKQYALKHIICNDVESLELALKEISVMKSLRGHPNVVTLYAHTVVDMGRTKEVLIVMEFCEKSLVNVLESRGAEFFDETQVLFIFRDVCNAVFAMHCRSPPIAHRDLKAENLLLGSDGHWKLCDFGSTSTNHKRFEKPEEMGIEEDNIRKHTTPAYRAPEVWFRVNNQLPVRSRKSLPDQPPDMQSTEKQEGEGEGALNLMNKSSPMPCRSPPPPPSVKSPSQAAHAPKATGGGGGGVGGGPLGAFWSTQHANETTNKDTSQIRFDEESTSRSTSKHERINPNNHSAVKNASPGDSNQKSNKTVSDSGSFRDFELSFFQNETGRSSNGAKASKPENAAFQDKAFNNFVAEFDTSKFSSDVTNNRPGKEVAASEAEVEKLKEQLKQANMEKAEITSKFEKLSAICRSQRQEIQELKQSLAARNIQPSAVPRVFPRFLFRPFDLVFQGKPPSLCIFYLTFQVQKADSNTRTPDAKPWQAFAGESPIQQPDTPEKGVKSVRTRSLPRKQASTEGTGFESWGFGSESFTAVSAGSSNASRLTGERSSSQRTDRASQPAGWAGF
Homology
BLAST of Carg07307 vs. NCBI nr
Match: KAG7036568.1 (putative serine/threonine-protein kinase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1190.6 bits (3079), Expect = 0.0e+00
Identity = 609/609 (100.00%), Postives = 609/609 (100.00%), Query Frame = 0

Query: 1   MWRFKPFMHKEPSGLEGRSIDVGNLKIHVRNVIAEGGFSCVYLAKDALHVSKQYALKHII 60
           MWRFKPFMHKEPSGLEGRSIDVGNLKIHVRNVIAEGGFSCVYLAKDALHVSKQYALKHII
Sbjct: 1   MWRFKPFMHKEPSGLEGRSIDVGNLKIHVRNVIAEGGFSCVYLAKDALHVSKQYALKHII 60

Query: 61  CNDVESLELALKEISVMKSLRGHPNVVTLYAHTVVDMGRTKEVLIVMEFCEKSLVNVLES 120
           CNDVESLELALKEISVMKSLRGHPNVVTLYAHTVVDMGRTKEVLIVMEFCEKSLVNVLES
Sbjct: 61  CNDVESLELALKEISVMKSLRGHPNVVTLYAHTVVDMGRTKEVLIVMEFCEKSLVNVLES 120

Query: 121 RGAEFFDETQVLFIFRDVCNAVFAMHCRSPPIAHRDLKAENLLLGSDGHWKLCDFGSTST 180
           RGAEFFDETQVLFIFRDVCNAVFAMHCRSPPIAHRDLKAENLLLGSDGHWKLCDFGSTST
Sbjct: 121 RGAEFFDETQVLFIFRDVCNAVFAMHCRSPPIAHRDLKAENLLLGSDGHWKLCDFGSTST 180

Query: 181 NHKRFEKPEEMGIEEDNIRKHTTPAYRAPEVWFRVNNQLPVRSRKSLPDQPPDMQSTEKQ 240
           NHKRFEKPEEMGIEEDNIRKHTTPAYRAPEVWFRVNNQLPVRSRKSLPDQPPDMQSTEKQ
Sbjct: 181 NHKRFEKPEEMGIEEDNIRKHTTPAYRAPEVWFRVNNQLPVRSRKSLPDQPPDMQSTEKQ 240

Query: 241 EGEGEGALNLMNKSSPMPCRSPPPPPSVKSPSQAAHAPKATGGGGGGVGGGPLGAFWSTQ 300
           EGEGEGALNLMNKSSPMPCRSPPPPPSVKSPSQAAHAPKATGGGGGGVGGGPLGAFWSTQ
Sbjct: 241 EGEGEGALNLMNKSSPMPCRSPPPPPSVKSPSQAAHAPKATGGGGGGVGGGPLGAFWSTQ 300

Query: 301 HANETTNKDTSQIRFDEESTSRSTSKHERINPNNHSAVKNASPGDSNQKSNKTVSDSGSF 360
           HANETTNKDTSQIRFDEESTSRSTSKHERINPNNHSAVKNASPGDSNQKSNKTVSDSGSF
Sbjct: 301 HANETTNKDTSQIRFDEESTSRSTSKHERINPNNHSAVKNASPGDSNQKSNKTVSDSGSF 360

Query: 361 RDFELSFFQNETGRSSNGAKASKPENAAFQDKAFNNFVAEFDTSKFSSDVTNNRPGKEVA 420
           RDFELSFFQNETGRSSNGAKASKPENAAFQDKAFNNFVAEFDTSKFSSDVTNNRPGKEVA
Sbjct: 361 RDFELSFFQNETGRSSNGAKASKPENAAFQDKAFNNFVAEFDTSKFSSDVTNNRPGKEVA 420

Query: 421 ASEAEVEKLKEQLKQANMEKAEITSKFEKLSAICRSQRQEIQELKQSLAARNIQPSAVPR 480
           ASEAEVEKLKEQLKQANMEKAEITSKFEKLSAICRSQRQEIQELKQSLAARNIQPSAVPR
Sbjct: 421 ASEAEVEKLKEQLKQANMEKAEITSKFEKLSAICRSQRQEIQELKQSLAARNIQPSAVPR 480

Query: 481 VFPRFLFRPFDLVFQGKPPSLCIFYLTFQVQKADSNTRTPDAKPWQAFAGESPIQQPDTP 540
           VFPRFLFRPFDLVFQGKPPSLCIFYLTFQVQKADSNTRTPDAKPWQAFAGESPIQQPDTP
Sbjct: 481 VFPRFLFRPFDLVFQGKPPSLCIFYLTFQVQKADSNTRTPDAKPWQAFAGESPIQQPDTP 540

Query: 541 EKGVKSVRTRSLPRKQASTEGTGFESWGFGSESFTAVSAGSSNASRLTGERSSSQRTDRA 600
           EKGVKSVRTRSLPRKQASTEGTGFESWGFGSESFTAVSAGSSNASRLTGERSSSQRTDRA
Sbjct: 541 EKGVKSVRTRSLPRKQASTEGTGFESWGFGSESFTAVSAGSSNASRLTGERSSSQRTDRA 600

Query: 601 SQPAGWAGF 610
           SQPAGWAGF
Sbjct: 601 SQPAGWAGF 609

BLAST of Carg07307 vs. NCBI nr
Match: XP_022949210.1 (probable serine/threonine-protein kinase DDB_G0276461 isoform X1 [Cucurbita moschata])

HSP 1 Score: 1038.5 bits (2684), Expect = 2.3e-299
Identity = 565/687 (82.24%), Postives = 570/687 (82.97%), Query Frame = 0

Query: 1   MWRFKPFMHKEPSGLEGRSIDVGNLKIHVRNVIAEGGFSCVYLAKDALHVSKQYALKHII 60
           MWRFKPFMHKEPSGLEGRSIDVGNLKIHVRNVIAEGGFSCVYLAKDALHVSKQYALKHII
Sbjct: 1   MWRFKPFMHKEPSGLEGRSIDVGNLKIHVRNVIAEGGFSCVYLAKDALHVSKQYALKHII 60

Query: 61  CNDVESLELALKEISVMKSLRGHPNVVTLYAHTVVDMGRTKEVLIVMEFCEKSLVNVLES 120
           CNDVESLELALKEISVMKSLRGHPNVVTLYAHT+VDMGRTKEVLIVMEFCEKSL NVLES
Sbjct: 61  CNDVESLELALKEISVMKSLRGHPNVVTLYAHTIVDMGRTKEVLIVMEFCEKSLFNVLES 120

Query: 121 RGAEFFDETQVLFIFRDVCNAVFAMHCRSPPIAHRDLKAENLLLGSDGHWKLCDFGSTST 180
           RGAE+FDETQVLFIFRDV NA+FAMHCRSPPIAHRDLKAENLLLGSDGHWKLCDFGSTST
Sbjct: 121 RGAEYFDETQVLFIFRDVVNAIFAMHCRSPPIAHRDLKAENLLLGSDGHWKLCDFGSTST 180

Query: 181 NHKRFEKPEEMGIEEDNIRKHTTPAYRAPE------------------------------ 240
           NHKRFEKPEEMGIEEDNIRKHTTPAYRAPE                              
Sbjct: 181 NHKRFEKPEEMGIEEDNIRKHTTPAYRAPEMWDLFRRELINEKVDIWALGCLLFRICYFK 240

Query: 241 ------------------------------------------------VWFRVNNQLPVR 300
                                                           VWFRVNNQLPVR
Sbjct: 241 SAFDGESKLQILNGNYRILELPKYSSSITDLIKDMLQASPNDRPDITQVWFRVNNQLPVR 300

Query: 301 SRKSLPDQPPDMQSTEKQEGEGEGALNLMNKSSPMPCRSPPPPPSVKSPSQAAHAPKATG 360
           SRKSLPDQPPDMQSTEKQE    GALNLMNKSSPMP RSPPPPPSVKSPSQAAHAPKATG
Sbjct: 301 SRKSLPDQPPDMQSTEKQEA---GALNLMNKSSPMPRRSPPPPPSVKSPSQAAHAPKATG 360

Query: 361 GGGGGVGGGPLGAFWSTQHANETTNKDTSQIRFDEESTSRSTSKHERINPNNHSAVKNAS 420
           GGGGGVGGGPLGAFWSTQHANETTNKDTSQIRFDEESTSRSTSKHERINPNNHSAVKNAS
Sbjct: 361 GGGGGVGGGPLGAFWSTQHANETTNKDTSQIRFDEESTSRSTSKHERINPNNHSAVKNAS 420

Query: 421 PGDSNQKSNKTVSDSGSFRDFELSFFQNETGRSSNGAKASKPENAAFQDKAFNNFVAEFD 480
           PGDSNQKSNKTVSDSGSFRDFELSFFQNETGRSSNGAKASKPENAAFQDKAFNNFVAEFD
Sbjct: 421 PGDSNQKSNKTVSDSGSFRDFELSFFQNETGRSSNGAKASKPENAAFQDKAFNNFVAEFD 480

Query: 481 TSKFSSDVTNNRPGKEVAASEAEVEKLKEQLKQANMEKAEITSKFEKLSAICRSQRQEIQ 540
           TS  SSDVTNNRPGKEVAASEAEVEKLKEQLKQANMEKAEITSKFEKLSAICRSQRQEIQ
Sbjct: 481 TS--SSDVTNNRPGKEVAASEAEVEKLKEQLKQANMEKAEITSKFEKLSAICRSQRQEIQ 540

Query: 541 ELKQSLAARNIQPSAVPRVFPRFLFRPFDLVFQGKPPSLCIFYLTFQVQKADSNTRTPDA 600
           ELKQSLAARNIQPSAVPR                             VQKA+SNTRTPDA
Sbjct: 541 ELKQSLAARNIQPSAVPR-----------------------------VQKANSNTRTPDA 600

Query: 601 KPWQAFAGESPIQQPDTPEKGVKSVRTRSLPRKQASTEGTGFESWGFGSESFTAVSAGSS 610
           KPWQAFAGESPIQQPDTPEKGVKSVRTRSLPRKQ STEGTGFESWGFGSESF+AVSAGSS
Sbjct: 601 KPWQAFAGESPIQQPDTPEKGVKSVRTRSLPRKQDSTEGTGFESWGFGSESFSAVSAGSS 653

BLAST of Carg07307 vs. NCBI nr
Match: XP_022949211.1 (probable serine/threonine-protein kinase DDB_G0276461 isoform X2 [Cucurbita moschata])

HSP 1 Score: 1038.1 bits (2683), Expect = 3.0e-299
Identity = 565/687 (82.24%), Postives = 570/687 (82.97%), Query Frame = 0

Query: 1   MWRFKPFMHKEPSGLEGRSIDVGNLKIHVRNVIAEGGFSCVYLAKDALHVSKQYALKHII 60
           MWRFKPFMHKEPSGLEGRSIDVGNLKIHVRNVIAEGGFSCVYLAKDALHVSKQYALKHII
Sbjct: 1   MWRFKPFMHKEPSGLEGRSIDVGNLKIHVRNVIAEGGFSCVYLAKDALHVSKQYALKHII 60

Query: 61  CNDVESLELALKEISVMKSLRGHPNVVTLYAHTVVDMGRTKEVLIVMEFCEKSLVNVLES 120
           CNDVESLELALKEISVMKSLRGHPNVVTLYAHT+VDMGRTKEVLIVMEFCEKSL NVLES
Sbjct: 61  CNDVESLELALKEISVMKSLRGHPNVVTLYAHTIVDMGRTKEVLIVMEFCEKSLFNVLES 120

Query: 121 RGAEFFDETQVLFIFRDVCNAVFAMHCRSPPIAHRDLKAENLLLGSDGHWKLCDFGSTST 180
           RGAE+FDETQVLFIFRDV NA+FAMHCRSPPIAHRDLKAENLLLGSDGHWKLCDFGSTST
Sbjct: 121 RGAEYFDETQVLFIFRDVVNAIFAMHCRSPPIAHRDLKAENLLLGSDGHWKLCDFGSTST 180

Query: 181 NHKRFEKPEEMGIEEDNIRKHTTPAYRAPE------------------------------ 240
           NHKRFEKPEEMGIEEDNIRKHTTPAYRAPE                              
Sbjct: 181 NHKRFEKPEEMGIEEDNIRKHTTPAYRAPEMWDLFRRELINEKVDIWALGCLLFRICYFK 240

Query: 241 ------------------------------------------------VWFRVNNQLPVR 300
                                                           VWFRVNNQLPVR
Sbjct: 241 SAFDGESKLQILNGNYRILELPKYSSSITDLIKDMLQASPNDRPDITQVWFRVNNQLPVR 300

Query: 301 SRKSLPDQPPDMQSTEKQEGEGEGALNLMNKSSPMPCRSPPPPPSVKSPSQAAHAPKATG 360
           SRKSLPDQPPDMQSTEKQ    EGALNLMNKSSPMP RSPPPPPSVKSPSQAAHAPKATG
Sbjct: 301 SRKSLPDQPPDMQSTEKQ----EGALNLMNKSSPMPRRSPPPPPSVKSPSQAAHAPKATG 360

Query: 361 GGGGGVGGGPLGAFWSTQHANETTNKDTSQIRFDEESTSRSTSKHERINPNNHSAVKNAS 420
           GGGGGVGGGPLGAFWSTQHANETTNKDTSQIRFDEESTSRSTSKHERINPNNHSAVKNAS
Sbjct: 361 GGGGGVGGGPLGAFWSTQHANETTNKDTSQIRFDEESTSRSTSKHERINPNNHSAVKNAS 420

Query: 421 PGDSNQKSNKTVSDSGSFRDFELSFFQNETGRSSNGAKASKPENAAFQDKAFNNFVAEFD 480
           PGDSNQKSNKTVSDSGSFRDFELSFFQNETGRSSNGAKASKPENAAFQDKAFNNFVAEFD
Sbjct: 421 PGDSNQKSNKTVSDSGSFRDFELSFFQNETGRSSNGAKASKPENAAFQDKAFNNFVAEFD 480

Query: 481 TSKFSSDVTNNRPGKEVAASEAEVEKLKEQLKQANMEKAEITSKFEKLSAICRSQRQEIQ 540
           TS  SSDVTNNRPGKEVAASEAEVEKLKEQLKQANMEKAEITSKFEKLSAICRSQRQEIQ
Sbjct: 481 TS--SSDVTNNRPGKEVAASEAEVEKLKEQLKQANMEKAEITSKFEKLSAICRSQRQEIQ 540

Query: 541 ELKQSLAARNIQPSAVPRVFPRFLFRPFDLVFQGKPPSLCIFYLTFQVQKADSNTRTPDA 600
           ELKQSLAARNIQPSAVPR                             VQKA+SNTRTPDA
Sbjct: 541 ELKQSLAARNIQPSAVPR-----------------------------VQKANSNTRTPDA 600

Query: 601 KPWQAFAGESPIQQPDTPEKGVKSVRTRSLPRKQASTEGTGFESWGFGSESFTAVSAGSS 610
           KPWQAFAGESPIQQPDTPEKGVKSVRTRSLPRKQ STEGTGFESWGFGSESF+AVSAGSS
Sbjct: 601 KPWQAFAGESPIQQPDTPEKGVKSVRTRSLPRKQDSTEGTGFESWGFGSESFSAVSAGSS 652

BLAST of Carg07307 vs. NCBI nr
Match: KAG6606862.1 (putative serine/threonine-protein kinase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1034.6 bits (2674), Expect = 3.3e-298
Identity = 566/682 (82.99%), Postives = 566/682 (82.99%), Query Frame = 0

Query: 8   MHKEPSGLEGRSIDVGNLKIHVRNVIAEGGFSCVYLAKDALHVSKQYALKHIICNDVESL 67
           MHKEPSGLEGRSIDVGNLKIHVRNVIAEGGFSCVYLAKDALHVSKQYALKHIICNDVESL
Sbjct: 1   MHKEPSGLEGRSIDVGNLKIHVRNVIAEGGFSCVYLAKDALHVSKQYALKHIICNDVESL 60

Query: 68  ELALKEISVMKSLRGHPNVVTLYAHTVVDMGRTKEVLIVMEFCEKSLVNVLESRGAEFFD 127
           ELALKEISVMKSLRGHPNVVTLYAHTVVDMGRTKEVLIVMEFCEKSLVNVLESRGAEFFD
Sbjct: 61  ELALKEISVMKSLRGHPNVVTLYAHTVVDMGRTKEVLIVMEFCEKSLVNVLESRGAEFFD 120

Query: 128 ETQVLFIFRDVCNAVFAMHCRSPPIAHRDLKAENLLLGSDGHWKLCDFGSTSTNHKRFEK 187
           ETQVLFIFRDVCNAVFAMHCRSPPIAHRDLKAENLLLGSDGHWKLCDFGSTSTNHKRFEK
Sbjct: 121 ETQVLFIFRDVCNAVFAMHCRSPPIAHRDLKAENLLLGSDGHWKLCDFGSTSTNHKRFEK 180

Query: 188 PEEMGIEEDNIRKHTTPAYRAPE------------------------------------- 247
           PEEMGIEEDNIRKHTTPAYRAPE                                     
Sbjct: 181 PEEMGIEEDNIRKHTTPAYRAPEMWDLFRRELINEKVDIWALGCLLFRICYFKSAFDGES 240

Query: 248 -----------------------------------------VWFRVNNQLPVRSRKSLPD 307
                                                    VWFRVNNQLPVRSRKSLPD
Sbjct: 241 KLQILNGNYRILELPKYSSSITDLIKDMLQASPNDRPDITQVWFRVNNQLPVRSRKSLPD 300

Query: 308 QPPDMQSTEKQEGEGEGALNLMNKSSPMPCRSPPPPPSVKSPSQAAHAPKAT--GGGGGG 367
           QPPDMQSTEKQ    EGALNLMNKSSPMP RSPPPPPSVKSPSQAAH PKAT  GGGGGG
Sbjct: 301 QPPDMQSTEKQ----EGALNLMNKSSPMPRRSPPPPPSVKSPSQAAHVPKATGGGGGGGG 360

Query: 368 VGGGPLGAFWSTQHANETTNKDTSQIRFDEESTSRSTSKHERINPNNHSAVKNASPGDSN 427
           VGGGPLGAFWSTQHANETTNKDTSQIRFDEESTSRSTSKHERINPNNHSAVKNASPGDSN
Sbjct: 361 VGGGPLGAFWSTQHANETTNKDTSQIRFDEESTSRSTSKHERINPNNHSAVKNASPGDSN 420

Query: 428 QKSNKTVSDSGSFRDFELSFFQNETGRSSNGAKASKPENAAFQDKAFNNFVAEFDTSKFS 487
           QKSNKTVSDSGSFRDFELSFFQNETGRSSNGAKASKPENAAFQDKAFNNFVAEFDTSKFS
Sbjct: 421 QKSNKTVSDSGSFRDFELSFFQNETGRSSNGAKASKPENAAFQDKAFNNFVAEFDTSKFS 480

Query: 488 SDVTNNRPGKEVAASEAEVEKLKEQLKQANMEKAEITSKFEKLSAICRSQRQEIQELKQS 547
           SDVTNNRPGKEVAASEAEVEKLKEQLKQANMEKAEITSKFEKLSAICRSQRQEIQELKQS
Sbjct: 481 SDVTNNRPGKEVAASEAEVEKLKEQLKQANMEKAEITSKFEKLSAICRSQRQEIQELKQS 540

Query: 548 LAARNIQPSAVPRVFPRFLFRPFDLVFQGKPPSLCIFYLTFQVQKADSNTRTPDAKPWQA 607
           LAARNIQPSAVPR                             VQKADSNTRTPDAKPWQA
Sbjct: 541 LAARNIQPSAVPR-----------------------------VQKADSNTRTPDAKPWQA 600

Query: 608 FAGESPIQQPDTPEKGVKSVRTRSLPRKQASTEGTGFESWGFGSESFTAVSAGSSNASRL 610
           FAGESPIQQPDTPEKGVKSVRTR LPRKQASTEGTGFESWGFGSESFTAVSAGSSNASRL
Sbjct: 601 FAGESPIQQPDTPEKGVKSVRTRRLPRKQASTEGTGFESWGFGSESFTAVSAGSSNASRL 649

BLAST of Carg07307 vs. NCBI nr
Match: XP_022949212.1 (probable serine/threonine-protein kinase DDB_G0276461 isoform X3 [Cucurbita moschata])

HSP 1 Score: 1011.5 bits (2614), Expect = 3.0e-291
Identity = 555/687 (80.79%), Postives = 560/687 (81.51%), Query Frame = 0

Query: 1   MWRFKPFMHKEPSGLEGRSIDVGNLKIHVRNVIAEGGFSCVYLAKDALHVSKQYALKHII 60
           MWRFKPFMHKEPSGLEGRSIDVGNLKIHVRNVIAEGGFSCVYLAKDALHVSKQYALKHII
Sbjct: 1   MWRFKPFMHKEPSGLEGRSIDVGNLKIHVRNVIAEGGFSCVYLAKDALHVSKQYALKHII 60

Query: 61  CNDVESLELALKEISVMKSLRGHPNVVTLYAHTVVDMGRTKEVLIVMEFCEKSLVNVLES 120
           CNDVESLELALKEISVMKSLRGHPNVVTLYAHT+VDMGRTKEVLIVMEFCEKSL NVLES
Sbjct: 61  CNDVESLELALKEISVMKSLRGHPNVVTLYAHTIVDMGRTKEVLIVMEFCEKSLFNVLES 120

Query: 121 RGAEFFDETQVLFIFRDVCNAVFAMHCRSPPIAHRDLKAENLLLGSDGHWKLCDFGSTST 180
           RGAE+FDETQVLFIFRDV NA+FAMHCRSPPIAHRDLKAENLLLGSDGHWKLCDFGSTST
Sbjct: 121 RGAEYFDETQVLFIFRDVVNAIFAMHCRSPPIAHRDLKAENLLLGSDGHWKLCDFGSTST 180

Query: 181 NHKRFEKPEEMGIEEDNIRKHTTPAYRAPE------------------------------ 240
           NHKRFEKPEEMGIEEDNIRKHTTPAYRAPE                              
Sbjct: 181 NHKRFEKPEEMGIEEDNIRKHTTPAYRAPEMWDLFRRELINEKVDIWALGCLLFRICYFK 240

Query: 241 ------------------------------------------------VWFRVNNQLPVR 300
                                                           VWFRVNNQLPVR
Sbjct: 241 SAFDGESKLQILNGNYRILELPKYSSSITDLIKDMLQASPNDRPDITQVWFRVNNQLPVR 300

Query: 301 SRKSLPDQPPDMQSTEKQEGEGEGALNLMNKSSPMPCRSPPPPPSVKSPSQAAHAPKATG 360
           SRKSLPDQPPDMQSTEKQE    GALNLMNKSSPMP RSPPPPPSVKSPSQAAHAPKATG
Sbjct: 301 SRKSLPDQPPDMQSTEKQEA---GALNLMNKSSPMPRRSPPPPPSVKSPSQAAHAPKATG 360

Query: 361 GGGGGVGGGPLGAFWSTQHANETTNKDTSQIRFDEESTSRSTSKHERINPNNHSAVKNAS 420
           GGGGGVGGGPLGAFWSTQHANETTNKDTSQIRFDEESTSRSTSKHERINPNNHSAVKNAS
Sbjct: 361 GGGGGVGGGPLGAFWSTQHANETTNKDTSQIRFDEESTSRSTSKHERINPNNHSAVKNAS 420

Query: 421 PGDSNQKSNKTVSDSGSFRDFELSFFQNETGRSSNGAKASKPENAAFQDKAFNNFVAEFD 480
           PGDSNQKSNKTVSDSGSFRDFELSFFQNETGRSSNGAKASKPENAAFQDKAFNNFVAEFD
Sbjct: 421 PGDSNQKSNKTVSDSGSFRDFELSFFQNETGRSSNGAKASKPENAAFQDKAFNNFVAEFD 480

Query: 481 TSKFSSDVTNNRPGKEVAASEAEVEKLKEQLKQANMEKAEITSKFEKLSAICRSQRQEIQ 540
           TS  SSDVTNNRPGKEVAASEAEVEKLKEQLKQANMEKAEITSKFEKLSAICRSQRQEIQ
Sbjct: 481 TS--SSDVTNNRPGKEVAASEAEVEKLKEQLKQANMEKAEITSKFEKLSAICRSQRQEIQ 540

Query: 541 ELKQSLAARNIQPSAVPRVFPRFLFRPFDLVFQGKPPSLCIFYLTFQVQKADSNTRTPDA 600
           ELKQSLAARNIQPSAVPR                             VQKA+SNTRTPDA
Sbjct: 541 ELKQSLAARNIQPSAVPR-----------------------------VQKANSNTRTPDA 600

Query: 601 KPWQAFAGESPIQQPDTPEKGVKSVRTRSLPRKQASTEGTGFESWGFGSESFTAVSAGSS 610
           KPW          QPDTPEKGVKSVRTRSLPRKQ STEGTGFESWGFGSESF+AVSAGSS
Sbjct: 601 KPW----------QPDTPEKGVKSVRTRSLPRKQDSTEGTGFESWGFGSESFSAVSAGSS 643

BLAST of Carg07307 vs. ExPASy Swiss-Prot
Match: Q54VV7 (Probable serine/threonine-protein kinase DDB_G0280111 OS=Dictyostelium discoideum OX=44689 GN=DDB_G0280111 PE=3 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 2.0e-35
Identity = 84/201 (41.79%), Postives = 125/201 (62.19%), Query Frame = 0

Query: 16  EGRSIDVGNLKIHVRNVIAEGGFSCVYLAKDALHVSKQYALKHIICNDVESLELALKEIS 75
           +G+++ +G+  ++    IAEGGFS V+L KD+ + SK YALK I+  D + L+    EIS
Sbjct: 5   QGKTLKIGSYHLNFVKQIAEGGFSYVFLVKDS-NTSKHYALKRILIRDEDELKGVKHEIS 64

Query: 76  VMKSLRGHPNVVTLYAHTVVDMGRTKEVLIVMEFCEKS-LVNVLESR---GAEFFDETQV 135
           +MK L  H N+V +  +  V      E+ I+ME+C    LV +++ R   G++F D+ ++
Sbjct: 65  IMKRLTKHKNIVKILDYHKVSDKNNTEMFILMEYCSGGHLVEIMQKRLSSGSKFTDQ-EI 124

Query: 136 LFIFRDVCNAVFAMHCRSPPIAHRDLKAENLLLGSD-GHWKLCDFGSTSTNHKRFEKPEE 195
           L IF+D+C +V  MH + P I HRDLK EN+LL  + G +KLCDFGS +    R +   E
Sbjct: 125 LKIFQDICESVAYMHSQQPLIIHRDLKVENVLLDEESGIYKLCDFGSATEEITRMKNKTE 184

Query: 196 MGIEEDNIRKHTTPAYRAPEV 212
           M   ED+I +HTT  YRAPE+
Sbjct: 185 MQNAEDDISRHTTLQYRAPEI 203

BLAST of Carg07307 vs. ExPASy Swiss-Prot
Match: Q3UHJ0 (AP2-associated protein kinase 1 OS=Mus musculus OX=10090 GN=Aak1 PE=1 SV=2)

HSP 1 Score: 147.5 bits (371), Expect = 4.9e-34
Identity = 84/204 (41.18%), Postives = 121/204 (59.31%), Query Frame = 0

Query: 13  SGLEGRSIDVGNLKIHVRNVIAEGGFSCVYLAKDALHVSKQYALKHIICNDVESLELALK 72
           SG  GR   +G  ++ V  V+AEGGF+ V+L + +  V  + ALK +  N+   L++  +
Sbjct: 32  SGYIGRVFGIGRQQVTVDEVLAEGGFALVFLVRTSNGV--KCALKRMFVNNEHDLQVCKR 91

Query: 73  EISVMKSLRGHPNVVTLYAHTV--VDMGRTKEVLIVMEFCE-KSLVNVLESRGAEFFDET 132
           EI +M+ L GH N+V     ++  V  G   EVLI+M+FC    +VN++  R    F E 
Sbjct: 92  EIQIMRDLSGHKNIVGYIDSSINNVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTEN 151

Query: 133 QVLFIFRDVCNAVFAMHCRSPPIAHRDLKAENLLLGSDGHWKLCDFGSTSTNHKRFEKPE 192
           +VL IF D C AV  +H    PI HRDLK EN+LL   GH+ LCDFGS +    +F+ P+
Sbjct: 152 EVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSAT---NKFQNPQ 211

Query: 193 EMGIE--EDNIRKHTTPAYRAPEV 212
             G+   ED I+K+TT +YRAPE+
Sbjct: 212 AEGVNAVEDEIKKYTTLSYRAPEM 230

BLAST of Carg07307 vs. ExPASy Swiss-Prot
Match: P0C1X8 (AP2-associated protein kinase 1 OS=Rattus norvegicus OX=10116 GN=Aak1 PE=1 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 4.9e-34
Identity = 84/204 (41.18%), Postives = 121/204 (59.31%), Query Frame = 0

Query: 13  SGLEGRSIDVGNLKIHVRNVIAEGGFSCVYLAKDALHVSKQYALKHIICNDVESLELALK 72
           SG  GR   +G  ++ V  V+AEGGF+ V+L + +  V  + ALK +  N+   L++  +
Sbjct: 32  SGYIGRVFGIGRQQVTVDEVLAEGGFALVFLVRTSNGV--KCALKRMFVNNEHDLQVCKR 91

Query: 73  EISVMKSLRGHPNVVTLYAHTV--VDMGRTKEVLIVMEFCE-KSLVNVLESRGAEFFDET 132
           EI +M+ L GH N+V     ++  V  G   EVLI+M+FC    +VN++  R    F E 
Sbjct: 92  EIQIMRDLSGHKNIVGYIDSSINNVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTEN 151

Query: 133 QVLFIFRDVCNAVFAMHCRSPPIAHRDLKAENLLLGSDGHWKLCDFGSTSTNHKRFEKPE 192
           +VL IF D C AV  +H    PI HRDLK EN+LL   GH+ LCDFGS +    +F+ P+
Sbjct: 152 EVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSAT---NKFQNPQ 211

Query: 193 EMGIE--EDNIRKHTTPAYRAPEV 212
             G+   ED I+K+TT +YRAPE+
Sbjct: 212 AEGVNAVEDEIKKYTTLSYRAPEM 230

BLAST of Carg07307 vs. ExPASy Swiss-Prot
Match: F1SPM8 (AP2-associated protein kinase 1 OS=Sus scrofa OX=9823 GN=AAK1 PE=2 SV=2)

HSP 1 Score: 146.7 bits (369), Expect = 8.3e-34
Identity = 83/204 (40.69%), Postives = 121/204 (59.31%), Query Frame = 0

Query: 13  SGLEGRSIDVGNLKIHVRNVIAEGGFSCVYLAKDALHVSKQYALKHIICNDVESLELALK 72
           SG  GR   +G  ++ V  V+AEGGF+ V+L + +     + ALK +  N+   L++  +
Sbjct: 32  SGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTS--NGMKCALKRMFVNNEHDLQVCKR 91

Query: 73  EISVMKSLRGHPNVVTLYAHTV--VDMGRTKEVLIVMEFCE-KSLVNVLESRGAEFFDET 132
           EI +M+ L GH N+V     ++  V  G   EVLI+M+FC    +VN++  R    F E+
Sbjct: 92  EIQIMRDLSGHKNIVGYIDSSINNVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTES 151

Query: 133 QVLFIFRDVCNAVFAMHCRSPPIAHRDLKAENLLLGSDGHWKLCDFGSTSTNHKRFEKPE 192
           +VL IF D C AV  +H    PI HRDLK EN+LL   GH+ LCDFGS +    +F+ P+
Sbjct: 152 EVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSAT---NKFQNPQ 211

Query: 193 EMGIE--EDNIRKHTTPAYRAPEV 212
             G+   ED I+K+TT +YRAPE+
Sbjct: 212 TEGVNAVEDEIKKYTTLSYRAPEM 230

BLAST of Carg07307 vs. ExPASy Swiss-Prot
Match: F1MH24 (AP2-associated protein kinase 1 OS=Bos taurus OX=9913 GN=AAK1 PE=1 SV=2)

HSP 1 Score: 146.4 bits (368), Expect = 1.1e-33
Identity = 83/204 (40.69%), Postives = 120/204 (58.82%), Query Frame = 0

Query: 13  SGLEGRSIDVGNLKIHVRNVIAEGGFSCVYLAKDALHVSKQYALKHIICNDVESLELALK 72
           SG  GR   +G  ++ V  V+AEGGF+ V+L + +     + ALK +  N+   L++  +
Sbjct: 32  SGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTS--NGMKCALKRMFVNNEHDLQVCKR 91

Query: 73  EISVMKSLRGHPNVVTLYAHTV--VDMGRTKEVLIVMEFCE-KSLVNVLESRGAEFFDET 132
           EI +M+ L GH N+V     ++  V  G   EVLI+M+FC    +VN++  R    F E 
Sbjct: 92  EIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTEN 151

Query: 133 QVLFIFRDVCNAVFAMHCRSPPIAHRDLKAENLLLGSDGHWKLCDFGSTSTNHKRFEKPE 192
           +VL IF D C AV  +H    PI HRDLK EN+LL   GH+ LCDFGS +    +F+ P+
Sbjct: 152 EVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSAT---NKFQNPQ 211

Query: 193 EMGIE--EDNIRKHTTPAYRAPEV 212
             G+   ED I+K+TT +YRAPE+
Sbjct: 212 TEGVNAVEDEIKKYTTLSYRAPEM 230

BLAST of Carg07307 vs. ExPASy TrEMBL
Match: A0A6J1GC51 (probable serine/threonine-protein kinase DDB_G0276461 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111452631 PE=4 SV=1)

HSP 1 Score: 1038.5 bits (2684), Expect = 1.1e-299
Identity = 565/687 (82.24%), Postives = 570/687 (82.97%), Query Frame = 0

Query: 1   MWRFKPFMHKEPSGLEGRSIDVGNLKIHVRNVIAEGGFSCVYLAKDALHVSKQYALKHII 60
           MWRFKPFMHKEPSGLEGRSIDVGNLKIHVRNVIAEGGFSCVYLAKDALHVSKQYALKHII
Sbjct: 1   MWRFKPFMHKEPSGLEGRSIDVGNLKIHVRNVIAEGGFSCVYLAKDALHVSKQYALKHII 60

Query: 61  CNDVESLELALKEISVMKSLRGHPNVVTLYAHTVVDMGRTKEVLIVMEFCEKSLVNVLES 120
           CNDVESLELALKEISVMKSLRGHPNVVTLYAHT+VDMGRTKEVLIVMEFCEKSL NVLES
Sbjct: 61  CNDVESLELALKEISVMKSLRGHPNVVTLYAHTIVDMGRTKEVLIVMEFCEKSLFNVLES 120

Query: 121 RGAEFFDETQVLFIFRDVCNAVFAMHCRSPPIAHRDLKAENLLLGSDGHWKLCDFGSTST 180
           RGAE+FDETQVLFIFRDV NA+FAMHCRSPPIAHRDLKAENLLLGSDGHWKLCDFGSTST
Sbjct: 121 RGAEYFDETQVLFIFRDVVNAIFAMHCRSPPIAHRDLKAENLLLGSDGHWKLCDFGSTST 180

Query: 181 NHKRFEKPEEMGIEEDNIRKHTTPAYRAPE------------------------------ 240
           NHKRFEKPEEMGIEEDNIRKHTTPAYRAPE                              
Sbjct: 181 NHKRFEKPEEMGIEEDNIRKHTTPAYRAPEMWDLFRRELINEKVDIWALGCLLFRICYFK 240

Query: 241 ------------------------------------------------VWFRVNNQLPVR 300
                                                           VWFRVNNQLPVR
Sbjct: 241 SAFDGESKLQILNGNYRILELPKYSSSITDLIKDMLQASPNDRPDITQVWFRVNNQLPVR 300

Query: 301 SRKSLPDQPPDMQSTEKQEGEGEGALNLMNKSSPMPCRSPPPPPSVKSPSQAAHAPKATG 360
           SRKSLPDQPPDMQSTEKQE    GALNLMNKSSPMP RSPPPPPSVKSPSQAAHAPKATG
Sbjct: 301 SRKSLPDQPPDMQSTEKQEA---GALNLMNKSSPMPRRSPPPPPSVKSPSQAAHAPKATG 360

Query: 361 GGGGGVGGGPLGAFWSTQHANETTNKDTSQIRFDEESTSRSTSKHERINPNNHSAVKNAS 420
           GGGGGVGGGPLGAFWSTQHANETTNKDTSQIRFDEESTSRSTSKHERINPNNHSAVKNAS
Sbjct: 361 GGGGGVGGGPLGAFWSTQHANETTNKDTSQIRFDEESTSRSTSKHERINPNNHSAVKNAS 420

Query: 421 PGDSNQKSNKTVSDSGSFRDFELSFFQNETGRSSNGAKASKPENAAFQDKAFNNFVAEFD 480
           PGDSNQKSNKTVSDSGSFRDFELSFFQNETGRSSNGAKASKPENAAFQDKAFNNFVAEFD
Sbjct: 421 PGDSNQKSNKTVSDSGSFRDFELSFFQNETGRSSNGAKASKPENAAFQDKAFNNFVAEFD 480

Query: 481 TSKFSSDVTNNRPGKEVAASEAEVEKLKEQLKQANMEKAEITSKFEKLSAICRSQRQEIQ 540
           TS  SSDVTNNRPGKEVAASEAEVEKLKEQLKQANMEKAEITSKFEKLSAICRSQRQEIQ
Sbjct: 481 TS--SSDVTNNRPGKEVAASEAEVEKLKEQLKQANMEKAEITSKFEKLSAICRSQRQEIQ 540

Query: 541 ELKQSLAARNIQPSAVPRVFPRFLFRPFDLVFQGKPPSLCIFYLTFQVQKADSNTRTPDA 600
           ELKQSLAARNIQPSAVPR                             VQKA+SNTRTPDA
Sbjct: 541 ELKQSLAARNIQPSAVPR-----------------------------VQKANSNTRTPDA 600

Query: 601 KPWQAFAGESPIQQPDTPEKGVKSVRTRSLPRKQASTEGTGFESWGFGSESFTAVSAGSS 610
           KPWQAFAGESPIQQPDTPEKGVKSVRTRSLPRKQ STEGTGFESWGFGSESF+AVSAGSS
Sbjct: 601 KPWQAFAGESPIQQPDTPEKGVKSVRTRSLPRKQDSTEGTGFESWGFGSESFSAVSAGSS 653

BLAST of Carg07307 vs. ExPASy TrEMBL
Match: A0A6J1GBD7 (probable serine/threonine-protein kinase DDB_G0276461 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111452631 PE=4 SV=1)

HSP 1 Score: 1038.1 bits (2683), Expect = 1.5e-299
Identity = 565/687 (82.24%), Postives = 570/687 (82.97%), Query Frame = 0

Query: 1   MWRFKPFMHKEPSGLEGRSIDVGNLKIHVRNVIAEGGFSCVYLAKDALHVSKQYALKHII 60
           MWRFKPFMHKEPSGLEGRSIDVGNLKIHVRNVIAEGGFSCVYLAKDALHVSKQYALKHII
Sbjct: 1   MWRFKPFMHKEPSGLEGRSIDVGNLKIHVRNVIAEGGFSCVYLAKDALHVSKQYALKHII 60

Query: 61  CNDVESLELALKEISVMKSLRGHPNVVTLYAHTVVDMGRTKEVLIVMEFCEKSLVNVLES 120
           CNDVESLELALKEISVMKSLRGHPNVVTLYAHT+VDMGRTKEVLIVMEFCEKSL NVLES
Sbjct: 61  CNDVESLELALKEISVMKSLRGHPNVVTLYAHTIVDMGRTKEVLIVMEFCEKSLFNVLES 120

Query: 121 RGAEFFDETQVLFIFRDVCNAVFAMHCRSPPIAHRDLKAENLLLGSDGHWKLCDFGSTST 180
           RGAE+FDETQVLFIFRDV NA+FAMHCRSPPIAHRDLKAENLLLGSDGHWKLCDFGSTST
Sbjct: 121 RGAEYFDETQVLFIFRDVVNAIFAMHCRSPPIAHRDLKAENLLLGSDGHWKLCDFGSTST 180

Query: 181 NHKRFEKPEEMGIEEDNIRKHTTPAYRAPE------------------------------ 240
           NHKRFEKPEEMGIEEDNIRKHTTPAYRAPE                              
Sbjct: 181 NHKRFEKPEEMGIEEDNIRKHTTPAYRAPEMWDLFRRELINEKVDIWALGCLLFRICYFK 240

Query: 241 ------------------------------------------------VWFRVNNQLPVR 300
                                                           VWFRVNNQLPVR
Sbjct: 241 SAFDGESKLQILNGNYRILELPKYSSSITDLIKDMLQASPNDRPDITQVWFRVNNQLPVR 300

Query: 301 SRKSLPDQPPDMQSTEKQEGEGEGALNLMNKSSPMPCRSPPPPPSVKSPSQAAHAPKATG 360
           SRKSLPDQPPDMQSTEKQ    EGALNLMNKSSPMP RSPPPPPSVKSPSQAAHAPKATG
Sbjct: 301 SRKSLPDQPPDMQSTEKQ----EGALNLMNKSSPMPRRSPPPPPSVKSPSQAAHAPKATG 360

Query: 361 GGGGGVGGGPLGAFWSTQHANETTNKDTSQIRFDEESTSRSTSKHERINPNNHSAVKNAS 420
           GGGGGVGGGPLGAFWSTQHANETTNKDTSQIRFDEESTSRSTSKHERINPNNHSAVKNAS
Sbjct: 361 GGGGGVGGGPLGAFWSTQHANETTNKDTSQIRFDEESTSRSTSKHERINPNNHSAVKNAS 420

Query: 421 PGDSNQKSNKTVSDSGSFRDFELSFFQNETGRSSNGAKASKPENAAFQDKAFNNFVAEFD 480
           PGDSNQKSNKTVSDSGSFRDFELSFFQNETGRSSNGAKASKPENAAFQDKAFNNFVAEFD
Sbjct: 421 PGDSNQKSNKTVSDSGSFRDFELSFFQNETGRSSNGAKASKPENAAFQDKAFNNFVAEFD 480

Query: 481 TSKFSSDVTNNRPGKEVAASEAEVEKLKEQLKQANMEKAEITSKFEKLSAICRSQRQEIQ 540
           TS  SSDVTNNRPGKEVAASEAEVEKLKEQLKQANMEKAEITSKFEKLSAICRSQRQEIQ
Sbjct: 481 TS--SSDVTNNRPGKEVAASEAEVEKLKEQLKQANMEKAEITSKFEKLSAICRSQRQEIQ 540

Query: 541 ELKQSLAARNIQPSAVPRVFPRFLFRPFDLVFQGKPPSLCIFYLTFQVQKADSNTRTPDA 600
           ELKQSLAARNIQPSAVPR                             VQKA+SNTRTPDA
Sbjct: 541 ELKQSLAARNIQPSAVPR-----------------------------VQKANSNTRTPDA 600

Query: 601 KPWQAFAGESPIQQPDTPEKGVKSVRTRSLPRKQASTEGTGFESWGFGSESFTAVSAGSS 610
           KPWQAFAGESPIQQPDTPEKGVKSVRTRSLPRKQ STEGTGFESWGFGSESF+AVSAGSS
Sbjct: 601 KPWQAFAGESPIQQPDTPEKGVKSVRTRSLPRKQDSTEGTGFESWGFGSESFSAVSAGSS 652

BLAST of Carg07307 vs. ExPASy TrEMBL
Match: A0A6J1GBE3 (probable serine/threonine-protein kinase DDB_G0276461 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111452631 PE=4 SV=1)

HSP 1 Score: 1011.5 bits (2614), Expect = 1.5e-291
Identity = 555/687 (80.79%), Postives = 560/687 (81.51%), Query Frame = 0

Query: 1   MWRFKPFMHKEPSGLEGRSIDVGNLKIHVRNVIAEGGFSCVYLAKDALHVSKQYALKHII 60
           MWRFKPFMHKEPSGLEGRSIDVGNLKIHVRNVIAEGGFSCVYLAKDALHVSKQYALKHII
Sbjct: 1   MWRFKPFMHKEPSGLEGRSIDVGNLKIHVRNVIAEGGFSCVYLAKDALHVSKQYALKHII 60

Query: 61  CNDVESLELALKEISVMKSLRGHPNVVTLYAHTVVDMGRTKEVLIVMEFCEKSLVNVLES 120
           CNDVESLELALKEISVMKSLRGHPNVVTLYAHT+VDMGRTKEVLIVMEFCEKSL NVLES
Sbjct: 61  CNDVESLELALKEISVMKSLRGHPNVVTLYAHTIVDMGRTKEVLIVMEFCEKSLFNVLES 120

Query: 121 RGAEFFDETQVLFIFRDVCNAVFAMHCRSPPIAHRDLKAENLLLGSDGHWKLCDFGSTST 180
           RGAE+FDETQVLFIFRDV NA+FAMHCRSPPIAHRDLKAENLLLGSDGHWKLCDFGSTST
Sbjct: 121 RGAEYFDETQVLFIFRDVVNAIFAMHCRSPPIAHRDLKAENLLLGSDGHWKLCDFGSTST 180

Query: 181 NHKRFEKPEEMGIEEDNIRKHTTPAYRAPE------------------------------ 240
           NHKRFEKPEEMGIEEDNIRKHTTPAYRAPE                              
Sbjct: 181 NHKRFEKPEEMGIEEDNIRKHTTPAYRAPEMWDLFRRELINEKVDIWALGCLLFRICYFK 240

Query: 241 ------------------------------------------------VWFRVNNQLPVR 300
                                                           VWFRVNNQLPVR
Sbjct: 241 SAFDGESKLQILNGNYRILELPKYSSSITDLIKDMLQASPNDRPDITQVWFRVNNQLPVR 300

Query: 301 SRKSLPDQPPDMQSTEKQEGEGEGALNLMNKSSPMPCRSPPPPPSVKSPSQAAHAPKATG 360
           SRKSLPDQPPDMQSTEKQE    GALNLMNKSSPMP RSPPPPPSVKSPSQAAHAPKATG
Sbjct: 301 SRKSLPDQPPDMQSTEKQEA---GALNLMNKSSPMPRRSPPPPPSVKSPSQAAHAPKATG 360

Query: 361 GGGGGVGGGPLGAFWSTQHANETTNKDTSQIRFDEESTSRSTSKHERINPNNHSAVKNAS 420
           GGGGGVGGGPLGAFWSTQHANETTNKDTSQIRFDEESTSRSTSKHERINPNNHSAVKNAS
Sbjct: 361 GGGGGVGGGPLGAFWSTQHANETTNKDTSQIRFDEESTSRSTSKHERINPNNHSAVKNAS 420

Query: 421 PGDSNQKSNKTVSDSGSFRDFELSFFQNETGRSSNGAKASKPENAAFQDKAFNNFVAEFD 480
           PGDSNQKSNKTVSDSGSFRDFELSFFQNETGRSSNGAKASKPENAAFQDKAFNNFVAEFD
Sbjct: 421 PGDSNQKSNKTVSDSGSFRDFELSFFQNETGRSSNGAKASKPENAAFQDKAFNNFVAEFD 480

Query: 481 TSKFSSDVTNNRPGKEVAASEAEVEKLKEQLKQANMEKAEITSKFEKLSAICRSQRQEIQ 540
           TS  SSDVTNNRPGKEVAASEAEVEKLKEQLKQANMEKAEITSKFEKLSAICRSQRQEIQ
Sbjct: 481 TS--SSDVTNNRPGKEVAASEAEVEKLKEQLKQANMEKAEITSKFEKLSAICRSQRQEIQ 540

Query: 541 ELKQSLAARNIQPSAVPRVFPRFLFRPFDLVFQGKPPSLCIFYLTFQVQKADSNTRTPDA 600
           ELKQSLAARNIQPSAVPR                             VQKA+SNTRTPDA
Sbjct: 541 ELKQSLAARNIQPSAVPR-----------------------------VQKANSNTRTPDA 600

Query: 601 KPWQAFAGESPIQQPDTPEKGVKSVRTRSLPRKQASTEGTGFESWGFGSESFTAVSAGSS 610
           KPW          QPDTPEKGVKSVRTRSLPRKQ STEGTGFESWGFGSESF+AVSAGSS
Sbjct: 601 KPW----------QPDTPEKGVKSVRTRSLPRKQDSTEGTGFESWGFGSESFSAVSAGSS 643

BLAST of Carg07307 vs. ExPASy TrEMBL
Match: A0A6J1KGP0 (probable serine/threonine-protein kinase DDB_G0276461 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111493052 PE=4 SV=1)

HSP 1 Score: 1003.0 bits (2592), Expect = 5.2e-289
Identity = 551/687 (80.20%), Postives = 558/687 (81.22%), Query Frame = 0

Query: 1   MWRFKPFMHKEPSGLEGRSIDVGNLKIHVRNVIAEGGFSCVYLAKDALHVSKQYALKHII 60
           MWRFKPFMHKEPSGLEGRSIDVGNLKIHVRNVIAEGGFSCVYLAKDA+HVSKQYALKHII
Sbjct: 1   MWRFKPFMHKEPSGLEGRSIDVGNLKIHVRNVIAEGGFSCVYLAKDAIHVSKQYALKHII 60

Query: 61  CNDVESLELALKEISVMKSLRGHPNVVTLYAHTVVDMGRTKEVLIVMEFCEKSLVNVLES 120
           CNDVESLELALKEISVMKSLRGHPNVVTLYAHT+VDMGRTKEVLIVMEFCEKSLVNVLES
Sbjct: 61  CNDVESLELALKEISVMKSLRGHPNVVTLYAHTIVDMGRTKEVLIVMEFCEKSLVNVLES 120

Query: 121 RGAEFFDETQVLFIFRDVCNAVFAMHCRSPPIAHRDLKAENLLLGSDGHWKLCDFGSTST 180
           RGAE+FDETQVLFIFRDVCNAVFAMHCRSPPIAHRDLKAENLLLGSDGHWKLCDFGSTST
Sbjct: 121 RGAEYFDETQVLFIFRDVCNAVFAMHCRSPPIAHRDLKAENLLLGSDGHWKLCDFGSTST 180

Query: 181 NHKRFEKPEEMGIEEDNIRKHTTPAYRAPE------------------------------ 240
           NHKRFEKPEEMGIEEDNIRKHTTPAYRAPE                              
Sbjct: 181 NHKRFEKPEEMGIEEDNIRKHTTPAYRAPEMWDLFRRELINEKVDIWALGCLLFRICYFK 240

Query: 241 ------------------------------------------------VWFRVNNQLPVR 300
                                                           VWFRVNNQLPVR
Sbjct: 241 SAFDGESKLQILNGNYRILELPKYSSSITDLIKDMLQASPNDRPDITQVWFRVNNQLPVR 300

Query: 301 SRKSLPDQPPDMQSTEKQEGEGEGALNLMNKSSPMPCRSPPPPPSVKSPSQAAHAPKATG 360
           SRKSLPDQPPDMQS+EKQE    GALNLMNK SPMP RSPPPPPSVKS SQAAHAPKATG
Sbjct: 301 SRKSLPDQPPDMQSSEKQEA---GALNLMNK-SPMPRRSPPPPPSVKSSSQAAHAPKATG 360

Query: 361 GGGGGVGGGPLGAFWSTQHANETTNKDTSQIRFDEESTSRSTSKHERINPNNHSAVKNAS 420
           GGGGGVGGGPLGAFWSTQHANETTNKDTSQIRFDEESTS STSK E+ NPNNHSAVKNAS
Sbjct: 361 GGGGGVGGGPLGAFWSTQHANETTNKDTSQIRFDEESTSHSTSKREKTNPNNHSAVKNAS 420

Query: 421 PGDSNQKSNKTVSDSGSFRDFELSFFQNETGRSSNGAKASKPENAAFQDKAFNNFVAEFD 480
            G+SNQKSNK V+DSGSFRDFELSFFQNETG SSNGAKASKPENAA QDKAFNNFVAEFD
Sbjct: 421 SGNSNQKSNKAVTDSGSFRDFELSFFQNETGCSSNGAKASKPENAASQDKAFNNFVAEFD 480

Query: 481 TSKFSSDVTNNRPGKEVAASEAEVEKLKEQLKQANMEKAEITSKFEKLSAICRSQRQEIQ 540
           TSKFSS VTNNRPGKEVAASEAEVEKLKEQLKQANMEKAEITSKFEKLSAICRSQRQEIQ
Sbjct: 481 TSKFSSGVTNNRPGKEVAASEAEVEKLKEQLKQANMEKAEITSKFEKLSAICRSQRQEIQ 540

Query: 541 ELKQSLAARNIQPSAVPRVFPRFLFRPFDLVFQGKPPSLCIFYLTFQVQKADSNTRTPDA 600
           ELKQSLAARNIQPSAVPR                             VQKADSNTRTPDA
Sbjct: 541 ELKQSLAARNIQPSAVPR-----------------------------VQKADSNTRTPDA 600

Query: 601 KPWQAFAGESPIQQPDTPEKGVKSVRTRSLPRKQASTEGTGFESWGFGSESFTAVSAGSS 610
           KPWQAFAGESPIQQPDTPEKGVKSVR R     QASTEGTGFESWGFG ESFTAVSAGSS
Sbjct: 601 KPWQAFAGESPIQQPDTPEKGVKSVRIR-----QASTEGTGFESWGFGYESFTAVSAGSS 649

BLAST of Carg07307 vs. ExPASy TrEMBL
Match: A0A6J1KE97 (probable serine/threonine-protein kinase DDB_G0276461 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111493052 PE=4 SV=1)

HSP 1 Score: 1002.7 bits (2591), Expect = 6.8e-289
Identity = 551/687 (80.20%), Postives = 558/687 (81.22%), Query Frame = 0

Query: 1   MWRFKPFMHKEPSGLEGRSIDVGNLKIHVRNVIAEGGFSCVYLAKDALHVSKQYALKHII 60
           MWRFKPFMHKEPSGLEGRSIDVGNLKIHVRNVIAEGGFSCVYLAKDA+HVSKQYALKHII
Sbjct: 1   MWRFKPFMHKEPSGLEGRSIDVGNLKIHVRNVIAEGGFSCVYLAKDAIHVSKQYALKHII 60

Query: 61  CNDVESLELALKEISVMKSLRGHPNVVTLYAHTVVDMGRTKEVLIVMEFCEKSLVNVLES 120
           CNDVESLELALKEISVMKSLRGHPNVVTLYAHT+VDMGRTKEVLIVMEFCEKSLVNVLES
Sbjct: 61  CNDVESLELALKEISVMKSLRGHPNVVTLYAHTIVDMGRTKEVLIVMEFCEKSLVNVLES 120

Query: 121 RGAEFFDETQVLFIFRDVCNAVFAMHCRSPPIAHRDLKAENLLLGSDGHWKLCDFGSTST 180
           RGAE+FDETQVLFIFRDVCNAVFAMHCRSPPIAHRDLKAENLLLGSDGHWKLCDFGSTST
Sbjct: 121 RGAEYFDETQVLFIFRDVCNAVFAMHCRSPPIAHRDLKAENLLLGSDGHWKLCDFGSTST 180

Query: 181 NHKRFEKPEEMGIEEDNIRKHTTPAYRAPE------------------------------ 240
           NHKRFEKPEEMGIEEDNIRKHTTPAYRAPE                              
Sbjct: 181 NHKRFEKPEEMGIEEDNIRKHTTPAYRAPEMWDLFRRELINEKVDIWALGCLLFRICYFK 240

Query: 241 ------------------------------------------------VWFRVNNQLPVR 300
                                                           VWFRVNNQLPVR
Sbjct: 241 SAFDGESKLQILNGNYRILELPKYSSSITDLIKDMLQASPNDRPDITQVWFRVNNQLPVR 300

Query: 301 SRKSLPDQPPDMQSTEKQEGEGEGALNLMNKSSPMPCRSPPPPPSVKSPSQAAHAPKATG 360
           SRKSLPDQPPDMQS+EKQ    EGALNLMNK SPMP RSPPPPPSVKS SQAAHAPKATG
Sbjct: 301 SRKSLPDQPPDMQSSEKQ----EGALNLMNK-SPMPRRSPPPPPSVKSSSQAAHAPKATG 360

Query: 361 GGGGGVGGGPLGAFWSTQHANETTNKDTSQIRFDEESTSRSTSKHERINPNNHSAVKNAS 420
           GGGGGVGGGPLGAFWSTQHANETTNKDTSQIRFDEESTS STSK E+ NPNNHSAVKNAS
Sbjct: 361 GGGGGVGGGPLGAFWSTQHANETTNKDTSQIRFDEESTSHSTSKREKTNPNNHSAVKNAS 420

Query: 421 PGDSNQKSNKTVSDSGSFRDFELSFFQNETGRSSNGAKASKPENAAFQDKAFNNFVAEFD 480
            G+SNQKSNK V+DSGSFRDFELSFFQNETG SSNGAKASKPENAA QDKAFNNFVAEFD
Sbjct: 421 SGNSNQKSNKAVTDSGSFRDFELSFFQNETGCSSNGAKASKPENAASQDKAFNNFVAEFD 480

Query: 481 TSKFSSDVTNNRPGKEVAASEAEVEKLKEQLKQANMEKAEITSKFEKLSAICRSQRQEIQ 540
           TSKFSS VTNNRPGKEVAASEAEVEKLKEQLKQANMEKAEITSKFEKLSAICRSQRQEIQ
Sbjct: 481 TSKFSSGVTNNRPGKEVAASEAEVEKLKEQLKQANMEKAEITSKFEKLSAICRSQRQEIQ 540

Query: 541 ELKQSLAARNIQPSAVPRVFPRFLFRPFDLVFQGKPPSLCIFYLTFQVQKADSNTRTPDA 600
           ELKQSLAARNIQPSAVPR                             VQKADSNTRTPDA
Sbjct: 541 ELKQSLAARNIQPSAVPR-----------------------------VQKADSNTRTPDA 600

Query: 601 KPWQAFAGESPIQQPDTPEKGVKSVRTRSLPRKQASTEGTGFESWGFGSESFTAVSAGSS 610
           KPWQAFAGESPIQQPDTPEKGVKSVR R     QASTEGTGFESWGFG ESFTAVSAGSS
Sbjct: 601 KPWQAFAGESPIQQPDTPEKGVKSVRIR-----QASTEGTGFESWGFGYESFTAVSAGSS 648

BLAST of Carg07307 vs. TAIR 10
Match: AT2G32850.2 (Protein kinase superfamily protein )

HSP 1 Score: 499.2 bits (1284), Expect = 4.7e-141
Identity = 330/714 (46.22%), Postives = 414/714 (57.98%), Query Frame = 0

Query: 1   MWRFKPFMHKEPSGLEGRSIDVGNLKIHVRNVIAEGGFSCVYLAKDALHVSKQYALKHII 60
           MW+FKPF  KEP+GLEGR +++GNLK+ VRNVIAEGGFS VYLA+D  H SKQYALKH+I
Sbjct: 1   MWKFKPFAQKEPAGLEGRFLEIGNLKVQVRNVIAEGGFSSVYLAQDVNHASKQYALKHMI 60

Query: 61  CNDVESLELALKEISVMKSLRGHPNVVTLYAHTVVDMGRT-KEVLIVMEFCEKSLVNVLE 120
           CND ESLEL +KEISV+KSL+GHPNVVTLYAH ++DMGR  KE L+ M+FC KSLV+VLE
Sbjct: 61  CNDEESLELVMKEISVLKSLKGHPNVVTLYAHGILDMGRNKKEALLAMDFCGKSLVDVLE 120

Query: 121 SRGAEFFDETQVLFIFRDVCNAVFAMHCRSPPIAHRDLKAENLLLGSDGHWKLCDFGSTS 180
           +RGA +F+E Q L IFRDVCNAVFAMHC+SP IAHRDLKAENLLL SDG WKLCDFGS S
Sbjct: 121 NRGAGYFEEKQALTIFRDVCNAVFAMHCQSPRIAHRDLKAENLLLSSDGQWKLCDFGSVS 180

Query: 181 TNHKRFEKPEEMGIEEDNIRKHTTPAYRAPE----------------------------- 240
           TNHK FE+ EEMGIEEDNIRK+TTP YRAPE                             
Sbjct: 181 TNHKIFERAEEMGIEEDNIRKYTTPTYRAPEMWDLFRREMISEKVDIWALGCLLFRICYF 240

Query: 241 -------------------------------------------------VWFRVNNQLPV 300
                                                            +WFRVN QLP 
Sbjct: 241 KNAFDGESKLQILNGNYRIPESPKYSVFITDLIKEMLQASPDERPDITQIWFRVNEQLPA 300

Query: 301 RSRKSLPDQPPDMQSTEKQEGEGEGALNLMNKSSPMPCRSPPPPPSVKSPSQAAHAPKAT 360
             +KSLPD+PP+MQST   +G  + A     K SP P RSPPPPP    PS         
Sbjct: 301 NLQKSLPDRPPEMQSTGVHDGSSKSA----TKPSPAPRRSPPPPP----PS--------- 360

Query: 361 GGGGGGVGGGPLGAFWSTQHANET--TNKDTSQIRFDEESTSRSTSKHERINPNNHSAVK 420
              G    GGPLGAFW+TQHA  +  +  + +  +FDE ++  +TSK ER+  ++H   K
Sbjct: 361 --SGESDSGGPLGAFWATQHAKTSVVSEDNKNMPKFDEPNS--NTSKSERVRVDSHQP-K 420

Query: 421 NASPGDSNQKSNKTVSDSGSFRDFELSFFQNET--GRSSNGAKASKPENAAFQDKAFNNF 480
             SP     +  +        +D E +  Q  T    ++N  + SK       D AFN+F
Sbjct: 421 KPSPVRGEARGIQ------RNKDLETTISQKNTTPAAANNMTRVSK-------DDAFNSF 480

Query: 481 VAEFDTSKFSSDVTNNRPGKEVAASEAEVEKLKEQLKQANMEKAEITSKFEKLSAICRSQ 540
           VA+FDT+KF +    N+PGKE  A EAE+++LK++LKQ   EKAEIT+KFEKLSAICRSQ
Sbjct: 481 VADFDTTKFDN---GNKPGKE-EALEAEIQRLKDELKQTKSEKAEITAKFEKLSAICRSQ 540

Query: 541 RQEIQELKQSLAARNIQPSAVPRVFPRFLFRPFDLVFQGKPPSLCIFYLTFQVQKADSN- 600
           RQE+Q+LKQ+LA+++  PS   R   +    P        P    +    +++Q+  SN 
Sbjct: 541 RQELQDLKQTLASKSASPSP-SRDSSQNQPSPGMHSMSSTPSRDKMEGTMWELQQDRSNW 600

Query: 601 -TRTPDAKPWQAFAGES-PIQQPDTPEKG-----VKSVRTRSLPRKQASTEGTGFESWGF 610
            T + D   WQ F+ E+ P+   ++  KG      +SVRTRS P   A T+  GFE WGF
Sbjct: 601 STGSSDTNSWQPFSDEAKPVM--ESASKGNNNTINQSVRTRSKPASAAGTQ--GFEPWGF 660

BLAST of Carg07307 vs. TAIR 10
Match: AT2G32850.1 (Protein kinase superfamily protein )

HSP 1 Score: 487.3 bits (1253), Expect = 1.8e-137
Identity = 321/709 (45.28%), Postives = 405/709 (57.12%), Query Frame = 0

Query: 1   MWRFKPFMHKEPSGLEGRSIDVGNLKIHVRNVIAEGGFSCVYLAKDALHVSKQYALKHII 60
           MW+FKPF  KEP+GLEGR +++GNLK+ VRNVIAEGGFS VYLA+D  H SKQYALKH+I
Sbjct: 1   MWKFKPFAQKEPAGLEGRFLEIGNLKVQVRNVIAEGGFSSVYLAQDVNHASKQYALKHMI 60

Query: 61  CNDVESLELALKEISVMKSLRGHPNVVTLYAHTVVDMGRT-KEVLIVMEFCEKSLVNVLE 120
           CND ESLEL +KEISV+KSL+GHPNVVTLYAH ++DMGR  KE L+ M+FC KSLV+VLE
Sbjct: 61  CNDEESLELVMKEISVLKSLKGHPNVVTLYAHGILDMGRNKKEALLAMDFCGKSLVDVLE 120

Query: 121 SRGAEFFDETQVLFIFRDVCNAVFAMHCRSPPIAHRDLKAENLLLGSDGHWKLCDFGSTS 180
           +RGA +F+E Q L IFRDVCNAVFAMHC+SP IAHRDLKAENLLL SDG WKLCDFGS S
Sbjct: 121 NRGAGYFEEKQALTIFRDVCNAVFAMHCQSPRIAHRDLKAENLLLSSDGQWKLCDFGSVS 180

Query: 181 TNHKRFEKPEEMGIEEDNIRKHTTPAYRAPE----------------------------- 240
           TNHK FE+ EEMGIEEDNIRK+TTP YRAPE                             
Sbjct: 181 TNHKIFERAEEMGIEEDNIRKYTTPTYRAPEMWDLFRREMISEKVDIWALGCLLFRICYF 240

Query: 241 -------------------------------------------------VWFRVNNQLPV 300
                                                            +WFRVN QLP 
Sbjct: 241 KNAFDGESKLQILNGNYRIPESPKYSVFITDLIKEMLQASPDERPDITQIWFRVNEQLPA 300

Query: 301 RSRKSLPDQPPDMQSTEKQEGEGEGALNLMNKSSPMPCRSPPPPPSVKSPSQAAHAPKAT 360
             +KSLPD+PP+MQST   +G  + A     K SP P RSPPPPP    PS         
Sbjct: 301 NLQKSLPDRPPEMQSTGVHDGSSKSA----TKPSPAPRRSPPPPP----PS--------- 360

Query: 361 GGGGGGVGGGPLGAFWSTQHANET--TNKDTSQIRFDEESTSRSTSKHERINPNNHSAVK 420
              G    GGPLGAFW+TQHA  +  +  + +  +FDE ++  +TSK ER+  ++H   K
Sbjct: 361 --SGESDSGGPLGAFWATQHAKTSVVSEDNKNMPKFDEPNS--NTSKSERVRVDSHQP-K 420

Query: 421 NASPGDSNQKSNKTVSDSGSFRDFELSFFQNET--GRSSNGAKASKPENAAFQDKAFNNF 480
             SP     +  +        +D E +  Q  T    ++N  + SK       D AFN+F
Sbjct: 421 KPSPVRGEARGIQ------RNKDLETTISQKNTTPAAANNMTRVSK-------DDAFNSF 480

Query: 481 VAEFDTSKFSSDVTNNRPGKEVAASEAEVEKLKEQLKQANMEKAEITSKFEKLSAICRSQ 540
           VA+FDT+KF +    N+PGKE  A EAE+++LK++LKQ   EKAEIT+KFEKLSAICRSQ
Sbjct: 481 VADFDTTKFDN---GNKPGKE-EALEAEIQRLKDELKQTKSEKAEITAKFEKLSAICRSQ 540

Query: 541 RQEIQELKQSLAARNIQPSAVPRVFPRFLFRPFDLVFQGKPPSLCIFYLTFQVQKADSN- 600
           RQE+Q+LKQ+LA+++  PS   R   +    P        P    +    +++Q+  SN 
Sbjct: 541 RQELQDLKQTLASKSASPSP-SRDSSQNQPSPGMHSMSSTPSRDKMEGTMWELQQDRSNW 600

Query: 601 -TRTPDAKPWQAFAGESPIQQPDTPEKGVKSVRTRSLPRKQASTEGT-GFESWGFGSESF 610
            T + D   WQ F+ E+                    P  +++++GT GFE WGF +ESF
Sbjct: 601 STGSSDTNSWQPFSDEAK-------------------PVMESASKGTQGFEPWGFETESF 650

BLAST of Carg07307 vs. TAIR 10
Match: AT5G08160.1 (serine/threonine protein kinase 3 )

HSP 1 Score: 88.6 bits (218), Expect = 1.9e-17
Identity = 66/231 (28.57%), Postives = 104/231 (45.02%), Query Frame = 0

Query: 17  GRSIDVGNLKIHVRNVIAEGGFSCVYL--------------------AKDALHVSKQ--Y 76
           G  + +   +  +   + EGGF+ V+L                     KD  H+S    Y
Sbjct: 18  GGDVWINENRFRIVRQLGEGGFAFVFLVKEIVADASSAASGGGLAKKVKDPAHLSADGTY 77

Query: 77  ALKHIICNDVESLELALKEISVMKSLRGHPNVVTLYAHTVVDMGRTKE-------VLIVM 136
           A+K ++  + E LEL  +EI V  SL  HPN++ L  H ++ +   +E        L+  
Sbjct: 78  AMKKVLIQNKEQLELVREEIRV-SSLFNHPNLLPLLDHAIISVKDGQEGAWKHEAFLLFP 137

Query: 137 EFCEKSLVN--VLESRGAEFFDETQVLFIFRDVCNAVFAMHCRSPPIAHRDLKAENLLL- 196
              + +L++   L     E F  T VL IFR +C+ +  MH   PP AH D+K  N+LL 
Sbjct: 138 VHLDGTLLDNFTLMKAKKETFSTTDVLHIFRQLCDGLKHMHSLEPPYAHNDVKPGNVLLT 197

Query: 197 ---GSDGHWKLCDFGSTSTNHKRFEKPEEMGIEEDNIRKHTTPAYRAPEVW 213
              G      L DFGS   + K+    +E    ++   +H +  +RAPE+W
Sbjct: 198 RRKGQPPLAILMDFGSARPSRKQIRSRQEALQLQEWTSEHCSAPFRAPELW 247

BLAST of Carg07307 vs. TAIR 10
Match: AT5G08160.2 (serine/threonine protein kinase 3 )

HSP 1 Score: 82.8 bits (203), Expect = 1.0e-15
Identity = 58/182 (31.87%), Postives = 89/182 (48.90%), Query Frame = 0

Query: 46  DALHVSKQ--YALKHIICNDVESLELALKEISVMKSLRGHPNVVTLYAHTVVDMGRTKE- 105
           D  H+S    YA+K ++  + E LEL  +EI V  SL  HPN++ L  H ++ +   +E 
Sbjct: 31  DPAHLSADGTYAMKKVLIQNKEQLELVREEIRV-SSLFNHPNLLPLLDHAIISVKDGQEG 90

Query: 106 ------VLIVMEFCEKSLVN--VLESRGAEFFDETQVLFIFRDVCNAVFAMHCRSPPIAH 165
                  L+     + +L++   L     E F  T VL IFR +C+ +  MH   PP AH
Sbjct: 91  AWKHEAFLLFPVHLDGTLLDNFTLMKAKKETFSTTDVLHIFRQLCDGLKHMHSLEPPYAH 150

Query: 166 RDLKAENLLL----GSDGHWKLCDFGSTSTNHKRFEKPEEMGIEEDNIRKHTTPAYRAPE 213
            D+K  N+LL    G      L DFGS   + K+    +E    ++   +H +  +RAPE
Sbjct: 151 NDVKPGNVLLTRRKGQPPLAILMDFGSARPSRKQIRSRQEALQLQEWTSEHCSAPFRAPE 210

BLAST of Carg07307 vs. TAIR 10
Match: AT5G03730.2 (Protein kinase superfamily protein )

HSP 1 Score: 80.1 bits (196), Expect = 6.8e-15
Identity = 66/202 (32.67%), Postives = 101/202 (50.00%), Query Frame = 0

Query: 14  GLEGRSIDVGNLKIHVRNVIAEGGFSCVYLAKDALHVSKQYALKHIICND--VESLELAL 73
           GL+G  +D+    ++++  I  G F  V+ A+   H S   A+K ++  D   E +   L
Sbjct: 538 GLDGDDMDIPWCDLNIKEKIGAGSFGTVHRAE--WHGS-DVAVKILMEQDFHAERVNEFL 597

Query: 74  KEISVMKSLRGHPNVVTLYAHTVVDMGRTKEVLIVMEFCEK-SLVNVLESRGA-EFFDET 133
           +E+++MK LR HPN+V L+   V    +   + IV E+  + SL  +L   GA E  DE 
Sbjct: 598 REVAIMKRLR-HPNIV-LFMGAVT---QPPNLSIVTEYLSRGSLYRLLHKSGAREQLDER 657

Query: 134 QVLFIFRDVCNAVFAMHCRSPPIAHRDLKAENLLLGSDGHWKLCDFGSTSTNHKRFEKPE 193
           + L +  DV   +  +H R+PPI HRDLK+ NLL+      K+CDFG +      F   +
Sbjct: 658 RRLSMAYDVAKGMNYLHNRNPPIVHRDLKSPNLLVDKKYTVKVCDFGLSRLKASTFLSSK 717

Query: 194 EMGIEEDNIRKHTTPAYRAPEV 212
                        TP + APEV
Sbjct: 718 SAA---------GTPEWMAPEV 722

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7036568.10.0e+00100.00putative serine/threonine-protein kinase, partial [Cucurbita argyrosperma subsp.... [more]
XP_022949210.12.3e-29982.24probable serine/threonine-protein kinase DDB_G0276461 isoform X1 [Cucurbita mosc... [more]
XP_022949211.13.0e-29982.24probable serine/threonine-protein kinase DDB_G0276461 isoform X2 [Cucurbita mosc... [more]
KAG6606862.13.3e-29882.99putative serine/threonine-protein kinase, partial [Cucurbita argyrosperma subsp.... [more]
XP_022949212.13.0e-29180.79probable serine/threonine-protein kinase DDB_G0276461 isoform X3 [Cucurbita mosc... [more]
Match NameE-valueIdentityDescription
Q54VV72.0e-3541.79Probable serine/threonine-protein kinase DDB_G0280111 OS=Dictyostelium discoideu... [more]
Q3UHJ04.9e-3441.18AP2-associated protein kinase 1 OS=Mus musculus OX=10090 GN=Aak1 PE=1 SV=2[more]
P0C1X84.9e-3441.18AP2-associated protein kinase 1 OS=Rattus norvegicus OX=10116 GN=Aak1 PE=1 SV=1[more]
F1SPM88.3e-3440.69AP2-associated protein kinase 1 OS=Sus scrofa OX=9823 GN=AAK1 PE=2 SV=2[more]
F1MH241.1e-3340.69AP2-associated protein kinase 1 OS=Bos taurus OX=9913 GN=AAK1 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1GC511.1e-29982.24probable serine/threonine-protein kinase DDB_G0276461 isoform X1 OS=Cucurbita mo... [more]
A0A6J1GBD71.5e-29982.24probable serine/threonine-protein kinase DDB_G0276461 isoform X2 OS=Cucurbita mo... [more]
A0A6J1GBE31.5e-29180.79probable serine/threonine-protein kinase DDB_G0276461 isoform X3 OS=Cucurbita mo... [more]
A0A6J1KGP05.2e-28980.20probable serine/threonine-protein kinase DDB_G0276461 isoform X1 OS=Cucurbita ma... [more]
A0A6J1KE976.8e-28980.20probable serine/threonine-protein kinase DDB_G0276461 isoform X2 OS=Cucurbita ma... [more]
Match NameE-valueIdentityDescription
AT2G32850.24.7e-14146.22Protein kinase superfamily protein [more]
AT2G32850.11.8e-13745.28Protein kinase superfamily protein [more]
AT5G08160.11.9e-1728.57serine/threonine protein kinase 3 [more]
AT5G08160.21.0e-1531.87serine/threonine protein kinase 3 [more]
AT5G03730.26.8e-1532.67Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 419..467
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 11..222
e-value: 2.9E-54
score: 186.3
NoneNo IPR availablePIRSRPIRSR037921-1PIRSR037921-1coord: 33..178
e-value: 1.2E-5
score: 22.6
NoneNo IPR availablePIRSRPIRSR000620-2PIRSR000620-2coord: 26..179
e-value: 0.006
score: 13.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 222..358
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 551..601
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 334..358
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 530..609
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 257..271
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 222..237
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 310..333
NoneNo IPR availablePANTHERPTHR22967SERINE/THREONINE PROTEIN KINASEcoord: 1..212
NoneNo IPR availablePANTHERPTHR22967:SF57NUMB-ASSOCIATED KINASE, ISOFORM Acoord: 211..604
coord: 1..212
NoneNo IPR availablePANTHERPTHR22967SERINE/THREONINE PROTEIN KINASEcoord: 211..604
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 27..394
e-value: 3.0E-22
score: 89.9
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 29..212
e-value: 5.7E-30
score: 104.6
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 27..338
score: 24.174099
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 152..164
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 28..214

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg07307-RACarg07307-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity