Carg07221 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg07221
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	LEC14B homolog
Location	Carg_Chr17: 8386247 .. 8388941 (+)
RNA-Seq Expression	Carg07221
Synteny	Carg07221

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATTATCCACTCCATGCTAAAAATTTGATGCATCCACGTGATCGATCTGTTGCTACATACAAGGGTCATTCAGTCCTGCGAACGCTTATCCGTTGTTATTTCTCCCCGGAATCTAGGTGAGCTTTTGTGGCTGCAACTGCATATTTATGACTGTATTACGGCCGAGATGCTAACTATTTGCTCTGCTGCATGACAATATTACCTGTGAAGTTTTGCATTATGCAGCAGTTTGTGCTATGCTATTTAAGCTTTGTTTCAAGGAAGTATTAATTTCATTCGATATCGGCTTCATTTTTCAATAAACCTTGTTCTATTGTGAATCGAATTTCTAGTTATCGTGCTAGATGCATTGAAGATGGTTAATAGTTGAGACTGTATTTTTATTTCCCCTCTGCAGCACCGGCCAGAAGTACATCTACACCGGATCTCACAATTCTTGCGTTTATATCTATGATTTGGTAAGTCATTTGACATTTATTTATATGCATCTTATTTTCACGTGAGGTTTTAATTTGACGTCGGAGCCGGGAAATTATTTAATTTATACCTCCGACCCTCCTTAGTAATATAACATCTATTAGTCCGATGCCTCGCACCTGGAAACATTTATTTATATTACTACTAATGTGCAAATGCAAGGGAGTTGGTTATACATAGGAGTGGAAGGGGAGGAAGGTAGGCAATGATTTAATTGTTTACGCTTAAGTGAGAATGCTCGAGAGGACGGTTCTGATAGAACCCGACATATATTGGATTAATGTGAGGTACTTGGACTCTCCCTCTTTTGACATCACTCAAGCTCTGATTCAACCGAAGGATTGCCTGCCTTCATCTTCTAAACCACTCTATTTATAACAAAACTCTCTTACAAACTCTGTCTAATTACTAACAGTCTCTTAATATCCCAATAACGTTCTTATCAGATTCTAAGTTCCCGGATATTGGGAGTTGCTCTACTGCTTTTGTGATATCCCACATTGGTTGGGGGGAGAGAATGAAACACCCTTTGTAAGGGGGTGGAAACCTTTCCCTAGCAGACTCGTTTTAAAGCCTTGAGGGGAAGCCCAAAAAGGACAATATCTGCTAGCGGTGGGCCTGGGCCTGGGCCGTTACAAATGGTATTAGAGCCAGACACCGGGCGATGTGTCAGGGAGAAGGCTGTTCCCTGAAGGGGAGTAGACACAAGGCGGTGTGTCAGTAAAGACGTTGGGCCCCGAAGGGGGTGGATTTGATGGGGTCCCACATCGATTAGAGAAAGGAACGAGTGCCAGCGAGGACGCTGGGCCCTGAAGGGGGGTGGATTGTGATATCCCACATTGGTTGGGGAGGAGAACGAAACACCCTTTATAAAGGAGTGGAAACCTTTCCTTAGCATACGTGTTTTAAAGCCTTGAGGGGAAGCCCGAAAGGGAAAGCCCAAAGAAGACAATATCTGCTAGCGGTGGGCCTGGCTGATAGAGCTTTAGCTTTTATATTTCAATACTTTGTTCTTTTGGTTTTTCATTGAAATCTCTTGCTGACTTTCTAGAACACGGGTCATAGCTGAATCACTGATTTACCTTAAAGTTTACGGAACTCTTTGTTAGTTAGCTCACTTGTTGTTTCTTATGCTGAACACAAGCGAGGAGAGTGTTAAGCAAATATTGAAAATATTAAATTCACCATTAGTTTAAATATTAGCTAAGTTGTTGGGTTCATTATTATATTATCATGGTTTTAGAGTTTAAACTCTTGGAATATCATTTTCTATTTAATTAGTATACAATAGTGTATGATCCCACATCGATTGGAGAGAGGAACGAGTGCCAGCAAGGACGTTGGACTCCGAAAGGGAAAACTCAAAGAGGACAATATCGGCTAGTAGTAGGCTTGGGCTATTACAAATGGTATCAGAGCCAGACATCAGGTAGTGTGCTAGTGAGGACGTTGGGCTCCAAAGGGGGTGGATTGTGAGATCCCACATCGATTGGAGAGAGAAACGAGTGCTAGCAAGGACGTTGGTCACGAAAGGGAAAGTCCAAAAAAGACAATATCTACTAGTGGTGGGCTTGAGCGGTTACAAATAGTTTTTTTGCGTTGGTTAAGCATTGGATTGTGAACCTATTTCTGGTTCTAGAAAATTGTATTCCATATATCTTATAACATTCTTATTTAGCATTATCTTTGTTGTTCTTATGTCCTTTGTAGTTGACTGGAGCTCTAGTTGCAACACTGAAGCATCATAAATCACCAGTAAGAGACTGTAGCTGGCACCCTCAATATCCAATGCTTGTAAGCTCTTCATGGGATGGGGATGTTGTCAAATGGGAGTTTCCAGGCAGCGGTGAAGCTCCGACTCCTCCGAACAAGAAGAGAGTCCGCAGGCGACATTACTATTGAAAACTGCTGAAGAAAACTGTAAGGCAAGCTCTTTCTGGTGGATTATAGCTTGGCAAATGCAGTATATCTTGCTCTGTGATGCATGCATCCTATCATACTCTTGAAAATAAGATGCAATTAGTATGCATTTTATATCTCACCGTTGCAGTATTTTGTTGGTTCTACTTGACGAATCTTTGATATCATGTTTCTGTCTATAATCTATGTGAATAATATGATATAATACATACATACACATATATATAGTGTATAAATTTGTTGAATAATGATCAACAAAATGGTGGAAATAGCAAGCATCGGTATATTCCTTCTT

mRNA sequence

ATGGATTATCCACTCCATGCTAAAAATTTGATGCATCCACGTGATCGATCTGTTGCTACATACAAGGGTCATTCAGTCCTGCGAACGCTTATCCGTTGTTATTTCTCCCCGGAATCTAGCACCGGCCAGAAGTACATCTACACCGGATCTCACAATTCTTGCGTTTATATCTATGATTTGTTGACTGGAGCTCTAGTTGCAACACTGAAGCATCATAAATCACCAGTAAGAGACTGTAGCTGGCACCCTCAATATCCAATGCTTGTAAGCTCTTCATGGGATGGGGATGTTGTCAAATGGGAGTTTCCAGGCAGCGGTGAAGCTCCGACTCCTCCGAACAAGAAGAGAGTCCGCAGGCGACATTACTATTGAAAACTGCTGAAGAAAACTGTAAGGCAAGCTCTTTCTGGTGGATTATAGCTTGGCAAATGCAGTATATCTTGCTCTGTGATGCATGCATCCTATCATACTCTTGAAAATAAGATGCAATTAGTATGCATTTTATATCTCACCGTTGCAGTATTTTGTTGGTTCTACTTGACGAATCTTTGATATCATGTTTCTGTCTATAATCTATGTGAATAATATGATATAATACATACATACACATATATATAGTGTATAAATTTGTTGAATAATGATCAACAAAATGGTGGAAATAGCAAGCATCGGTATATTCCTTCTT

Coding sequence (CDS)

ATGGATTATCCACTCCATGCTAAAAATTTGATGCATCCACGTGATCGATCTGTTGCTACATACAAGGGTCATTCAGTCCTGCGAACGCTTATCCGTTGTTATTTCTCCCCGGAATCTAGCACCGGCCAGAAGTACATCTACACCGGATCTCACAATTCTTGCGTTTATATCTATGATTTGTTGACTGGAGCTCTAGTTGCAACACTGAAGCATCATAAATCACCAGTAAGAGACTGTAGCTGGCACCCTCAATATCCAATGCTTGTAAGCTCTTCATGGGATGGGGATGTTGTCAAATGGGAGTTTCCAGGCAGCGGTGAAGCTCCGACTCCTCCGAACAAGAAGAGAGTCCGCAGGCGACATTACTATTGA

Protein sequence

MDYPLHAKNLMHPRDRSVATYKGHSVLRTLIRCYFSPESSTGQKYIYTGSHNSCVYIYDLLTGALVATLKHHKSPVRDCSWHPQYPMLVSSSWDGDVVKWEFPGSGEAPTPPNKKRVRRRHYY

Homology

[Close]

BLAST of Carg07221 vs. NCBI nr
Match: XP_022954198.1 (LEC14B protein [Cucurbita moschata] >XP_022954199.1 LEC14B protein [Cucurbita moschata] >XP_022954200.1 LEC14B protein [Cucurbita moschata] >KAG6575877.1 hypothetical protein SDJN03_26516, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 272.3 bits (695), Expect = 2.0e-69
Identity = 123/123 (100.00%), Postives = 123/123 (100.00%), Query Frame = 0


Query: 1   MDYPLHAKNLMHPRDRSVATYKGHSVLRTLIRCYFSPESSTGQKYIYTGSHNSCVYIYDL 60
           MDYPLHAKNLMHPRDRSVATYKGHSVLRTLIRCYFSPESSTGQKYIYTGSHNSCVYIYDL
Sbjct: 366 MDYPLHAKNLMHPRDRSVATYKGHSVLRTLIRCYFSPESSTGQKYIYTGSHNSCVYIYDL 425

Query: 61  LTGALVATLKHHKSPVRDCSWHPQYPMLVSSSWDGDVVKWEFPGSGEAPTPPNKKRVRRR 120
           LTGALVATLKHHKSPVRDCSWHPQYPMLVSSSWDGDVVKWEFPGSGEAPTPPNKKRVRRR
Sbjct: 426 LTGALVATLKHHKSPVRDCSWHPQYPMLVSSSWDGDVVKWEFPGSGEAPTPPNKKRVRRR 485

Query: 121 HYY 124
           HYY
Sbjct: 486 HYY 488

[Close]

BLAST of Carg07221 vs. NCBI nr
Match: KAG7014412.1 (hypothetical protein SDJN02_24589, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 272.3 bits (695), Expect = 2.0e-69
Identity = 123/123 (100.00%), Postives = 123/123 (100.00%), Query Frame = 0


Query: 1   MDYPLHAKNLMHPRDRSVATYKGHSVLRTLIRCYFSPESSTGQKYIYTGSHNSCVYIYDL 60
           MDYPLHAKNLMHPRDRSVATYKGHSVLRTLIRCYFSPESSTGQKYIYTGSHNSCVYIYDL
Sbjct: 1   MDYPLHAKNLMHPRDRSVATYKGHSVLRTLIRCYFSPESSTGQKYIYTGSHNSCVYIYDL 60

Query: 61  LTGALVATLKHHKSPVRDCSWHPQYPMLVSSSWDGDVVKWEFPGSGEAPTPPNKKRVRRR 120
           LTGALVATLKHHKSPVRDCSWHPQYPMLVSSSWDGDVVKWEFPGSGEAPTPPNKKRVRRR
Sbjct: 61  LTGALVATLKHHKSPVRDCSWHPQYPMLVSSSWDGDVVKWEFPGSGEAPTPPNKKRVRRR 120

Query: 121 HYY 124
           HYY
Sbjct: 121 HYY 123

[Close]

BLAST of Carg07221 vs. NCBI nr
Match: XP_023522133.1 (LEC14B protein-like [Cucurbita pepo subsp. pepo] >XP_023522135.1 LEC14B protein-like [Cucurbita pepo subsp. pepo] >XP_023522136.1 LEC14B protein-like [Cucurbita pepo subsp. pepo] >XP_023548472.1 LEC14B protein-like [Cucurbita pepo subsp. pepo] >XP_023548473.1 LEC14B protein-like [Cucurbita pepo subsp. pepo] >XP_023548474.1 LEC14B protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 270.8 bits (691), Expect = 5.9e-69
Identity = 122/123 (99.19%), Postives = 123/123 (100.00%), Query Frame = 0


Query: 1   MDYPLHAKNLMHPRDRSVATYKGHSVLRTLIRCYFSPESSTGQKYIYTGSHNSCVYIYDL 60
           MDYPLHAKNLMHPRDRSVATYKGHSVLRTLIRCYFSPESSTGQKYIYTGSHNSCVYIYDL
Sbjct: 366 MDYPLHAKNLMHPRDRSVATYKGHSVLRTLIRCYFSPESSTGQKYIYTGSHNSCVYIYDL 425

Query: 61  LTGALVATLKHHKSPVRDCSWHPQYPMLVSSSWDGDVVKWEFPGSGEAPTPPNKKRVRRR 120
           LTGALVATLKHHKSPVRDCSWHPQYPMLVSSSWDGDVVKWEFPGSGEAPTPPNKKRVRRR
Sbjct: 426 LTGALVATLKHHKSPVRDCSWHPQYPMLVSSSWDGDVVKWEFPGSGEAPTPPNKKRVRRR 485

Query: 121 HYY 124
           H+Y
Sbjct: 486 HFY 488

[Close]

BLAST of Carg07221 vs. NCBI nr
Match: XP_023548494.1 (LEC14B homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 270.8 bits (691), Expect = 5.9e-69
Identity = 122/123 (99.19%), Postives = 123/123 (100.00%), Query Frame = 0


Query: 1   MDYPLHAKNLMHPRDRSVATYKGHSVLRTLIRCYFSPESSTGQKYIYTGSHNSCVYIYDL 60
           MDYPLHAKNLMHPRDRSVATYKGHSVLRTLIRCYFSPESSTGQKYIYTGSHNSCVYIYDL
Sbjct: 180 MDYPLHAKNLMHPRDRSVATYKGHSVLRTLIRCYFSPESSTGQKYIYTGSHNSCVYIYDL 239

Query: 61  LTGALVATLKHHKSPVRDCSWHPQYPMLVSSSWDGDVVKWEFPGSGEAPTPPNKKRVRRR 120
           LTGALVATLKHHKSPVRDCSWHPQYPMLVSSSWDGDVVKWEFPGSGEAPTPPNKKRVRRR
Sbjct: 240 LTGALVATLKHHKSPVRDCSWHPQYPMLVSSSWDGDVVKWEFPGSGEAPTPPNKKRVRRR 299

Query: 121 HYY 124
           H+Y
Sbjct: 300 HFY 302

[Close]

BLAST of Carg07221 vs. NCBI nr
Match: XP_022992516.1 (LEC14B protein [Cucurbita maxima] >XP_022992517.1 LEC14B protein [Cucurbita maxima] >XP_022992518.1 LEC14B protein [Cucurbita maxima])

HSP 1 Score: 268.5 bits (685), Expect = 2.9e-68
Identity = 121/123 (98.37%), Postives = 122/123 (99.19%), Query Frame = 0


Query: 1   MDYPLHAKNLMHPRDRSVATYKGHSVLRTLIRCYFSPESSTGQKYIYTGSHNSCVYIYDL 60
           MDYPLHAKNLMHPRDRSVATYKGHSVLRTLIRCYFSPESSTGQKYIYTGSHNSCVYIYDL
Sbjct: 366 MDYPLHAKNLMHPRDRSVATYKGHSVLRTLIRCYFSPESSTGQKYIYTGSHNSCVYIYDL 425

Query: 61  LTGALVATLKHHKSPVRDCSWHPQYPMLVSSSWDGDVVKWEFPGSGEAPTPPNKKRVRRR 120
           LTG LVATLKHHKSPVRDCSWHPQYPMLVSSSWDGDVVKWEFPGSGEAPTPPNKKRVRRR
Sbjct: 426 LTGDLVATLKHHKSPVRDCSWHPQYPMLVSSSWDGDVVKWEFPGSGEAPTPPNKKRVRRR 485

Query: 121 HYY 124
           H+Y
Sbjct: 486 HFY 488

[Close]

BLAST of Carg07221 vs. ExPASy Swiss-Prot
Match: O24467 (LEC14B homolog OS=Prunus armeniaca OX=36596 PE=2 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 5.0e-47
Identity = 83/120 (69.17%), Postives = 95/120 (79.17%), Query Frame = 0


Query: 1   MDYPLHAKNLMHPRDRSVATYKGHSVLRTLIRCYFSPESSTGQKYIYTGSHNSCVYIYDL 60
           M+YP HAK L HP D+S+ATY+GH VLRTLIRCY SP  STGQKYIYTGS + CVYIYDL
Sbjct: 353 MEYPAHAKTLKHPNDQSLATYRGHGVLRTLIRCYLSPAYSTGQKYIYTGSSDHCVYIYDL 412

Query: 61  LTGALVATLKHHKSPVRDCSWHPQYPMLVSSSWDGDVVKWEFPGSGEAPTPPNKKRVRRR 120
           +TGA VA L HH+ PVRDCSWHP YPMLVSSSWDG + +WEFPG  + PT   + R RR+
Sbjct: 413 VTGAQVARLNHHEGPVRDCSWHPLYPMLVSSSWDGTIARWEFPGDDQVPT-LERPRARRK 471

[Close]

BLAST of Carg07221 vs. ExPASy Swiss-Prot
Match: Q40153 (LEC14B protein OS=Lithospermum erythrorhizon OX=34254 PE=2 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 4.7e-45
Identity = 81/119 (68.07%), Postives = 100/119 (84.03%), Query Frame = 0


Query: 1   MDYPLHAKNLMHPRDRSVATYKGHSVLRTLIRCYFSPESSTGQKYIYTGSHNSCVYIYDL 60
           M+YP  A++L HP D S ATYKGHSVL TLIRCYFSP+ STGQKYIYTGSH++ VYIYDL
Sbjct: 355 MEYPQEARDLKHPSDLSGATYKGHSVLCTLIRCYFSPDYSTGQKYIYTGSHDANVYIYDL 414

Query: 61  LTGALVATLKHHKSPVRDCSWHPQYPMLVSSSWDGDVVKWEFPGSGEAPTPPNKKRVRR 120
           +TG  V+TL++HK+ VRDCSWHP YPMLVSSS+DG++VKWE+ G+ EAP   N +R++R
Sbjct: 415 VTGDQVSTLQYHKATVRDCSWHPNYPMLVSSSFDGEIVKWEYRGNDEAPVQGNNQRLQR 473

[Close]

BLAST of Carg07221 vs. ExPASy Swiss-Prot
Match: Q91VU6 (DDB1- and CUL4-associated factor 11 OS=Mus musculus OX=10090 GN=Dcaf11 PE=1 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 1.6e-24
Identity = 51/95 (53.68%), Postives = 66/95 (69.47%), Query Frame = 0


Query: 8   KNLMHPRDRSVATYKGHSVLRTLIRCYFSPESSTGQKYIYTGSHNSCVYIYDLLTGALVA 67
           K L  P D S+ TY+GH VL TLIRC FSP  STGQ++IY+G     V +YDLL+G +V 
Sbjct: 418 KKLKLPGDSSLMTYRGHGVLHTLIRCRFSPAHSTGQQFIYSGCSTGKVVVYDLLSGHIVK 477

Query: 68  TLKHHKSPVRDCSWHPQYPMLVSSSWDGDVVKWEF 103
            L +HK+ VRD SWHP    +VSSSWDG++  W++
Sbjct: 478 KLTNHKACVRDVSWHPFEEKIVSSSWDGNLRLWQY 512

[Close]

BLAST of Carg07221 vs. ExPASy Swiss-Prot
Match: Q5M9G8 (DDB1- and CUL4-associated factor 11 OS=Rattus norvegicus OX=10116 GN=Dcaf11 PE=1 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 2.1e-24
Identity = 51/95 (53.68%), Postives = 65/95 (68.42%), Query Frame = 0


Query: 8   KNLMHPRDRSVATYKGHSVLRTLIRCYFSPESSTGQKYIYTGSHNSCVYIYDLLTGALVA 67
           K L  P D S+ TY+GH VL TLIRC FSP  STGQ++IY+G     V +YDLL+G +V 
Sbjct: 418 KKLKLPGDSSLMTYRGHGVLHTLIRCRFSPAHSTGQQFIYSGCSTGKVVVYDLLSGHIVK 477

Query: 68  TLKHHKSPVRDCSWHPQYPMLVSSSWDGDVVKWEF 103
            L +HK+ VRD SWHP    +VSSSWDG +  W++
Sbjct: 478 KLTNHKACVRDVSWHPFEEKIVSSSWDGSLRLWQY 512

[Close]

BLAST of Carg07221 vs. ExPASy Swiss-Prot
Match: Q5E9I8 (DDB1- and CUL4-associated factor 11 OS=Bos taurus OX=9913 GN=DCAF11 PE=2 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 6.0e-24
Identity = 55/115 (47.83%), Postives = 71/115 (61.74%), Query Frame = 0


Query: 8   KNLMHPRDRSVATYKGHSVLRTLIRCYFSPESSTGQKYIYTGSHNSCVYIYDLLTGALVA 67
           + L  P D S+ TY+GH VL TLIRC FSP  STGQ++IY+G     V +YDLL+G +V 
Sbjct: 418 RKLKLPGDSSLMTYRGHGVLHTLIRCRFSPTHSTGQQFIYSGCSTGKVVVYDLLSGHVVK 477

Query: 68  TLKHHKSPVRDCSWHPQYPMLVSSSWDGDVVKWEF----------PGSGEAPTPP 113
            L  HK+ VRD SWHP    +VSSSWDG++  W++          P S E P+ P
Sbjct: 478 KLTTHKACVRDVSWHPFEEKIVSSSWDGNLRLWQYRQAEYFQDDMPESEEHPSTP 532

[Close]

BLAST of Carg07221 vs. ExPASy TrEMBL
Match: A0A6J1GQE7 (LEC14B homolog OS=Cucurbita moschata OX=3662 GN=LOC111456529 PE=3 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 9.8e-70
Identity = 123/123 (100.00%), Postives = 123/123 (100.00%), Query Frame = 0


Query: 1   MDYPLHAKNLMHPRDRSVATYKGHSVLRTLIRCYFSPESSTGQKYIYTGSHNSCVYIYDL 60
           MDYPLHAKNLMHPRDRSVATYKGHSVLRTLIRCYFSPESSTGQKYIYTGSHNSCVYIYDL
Sbjct: 366 MDYPLHAKNLMHPRDRSVATYKGHSVLRTLIRCYFSPESSTGQKYIYTGSHNSCVYIYDL 425

Query: 61  LTGALVATLKHHKSPVRDCSWHPQYPMLVSSSWDGDVVKWEFPGSGEAPTPPNKKRVRRR 120
           LTGALVATLKHHKSPVRDCSWHPQYPMLVSSSWDGDVVKWEFPGSGEAPTPPNKKRVRRR
Sbjct: 426 LTGALVATLKHHKSPVRDCSWHPQYPMLVSSSWDGDVVKWEFPGSGEAPTPPNKKRVRRR 485

Query: 121 HYY 124
           HYY
Sbjct: 486 HYY 488

[Close]

BLAST of Carg07221 vs. ExPASy TrEMBL
Match: A0A6J1JQ33 (LEC14B homolog OS=Cucurbita maxima OX=3661 GN=LOC111488821 PE=3 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 1.4e-68
Identity = 121/123 (98.37%), Postives = 122/123 (99.19%), Query Frame = 0


Query: 1   MDYPLHAKNLMHPRDRSVATYKGHSVLRTLIRCYFSPESSTGQKYIYTGSHNSCVYIYDL 60
           MDYPLHAKNLMHPRDRSVATYKGHSVLRTLIRCYFSPESSTGQKYIYTGSHNSCVYIYDL
Sbjct: 366 MDYPLHAKNLMHPRDRSVATYKGHSVLRTLIRCYFSPESSTGQKYIYTGSHNSCVYIYDL 425

Query: 61  LTGALVATLKHHKSPVRDCSWHPQYPMLVSSSWDGDVVKWEFPGSGEAPTPPNKKRVRRR 120
           LTG LVATLKHHKSPVRDCSWHPQYPMLVSSSWDGDVVKWEFPGSGEAPTPPNKKRVRRR
Sbjct: 426 LTGDLVATLKHHKSPVRDCSWHPQYPMLVSSSWDGDVVKWEFPGSGEAPTPPNKKRVRRR 485

Query: 121 HYY 124
           H+Y
Sbjct: 486 HFY 488

[Close]

BLAST of Carg07221 vs. ExPASy TrEMBL
Match: A0A5D3D413 (LEC14B homolog OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G002170 PE=3 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 2.7e-67
Identity = 119/123 (96.75%), Postives = 120/123 (97.56%), Query Frame = 0


Query: 1   MDYPLHAKNLMHPRDRSVATYKGHSVLRTLIRCYFSPESSTGQKYIYTGSHNSCVYIYDL 60
           MDYP HAKNLMHPRDRSVATYKGHSVLRTLIRCYFSPE STGQKYIYTGSHNSCVYIYDL
Sbjct: 366 MDYPPHAKNLMHPRDRSVATYKGHSVLRTLIRCYFSPEYSTGQKYIYTGSHNSCVYIYDL 425

Query: 61  LTGALVATLKHHKSPVRDCSWHPQYPMLVSSSWDGDVVKWEFPGSGEAPTPPNKKRVRRR 120
           LTG LVATLKHHKSPVRDCSWHPQYPMLVSSSWDGDVVKWEFPGSGEAPTPPNKKRVRRR
Sbjct: 426 LTGVLVATLKHHKSPVRDCSWHPQYPMLVSSSWDGDVVKWEFPGSGEAPTPPNKKRVRRR 485

Query: 121 HYY 124
           H+Y
Sbjct: 486 HFY 488

[Close]

BLAST of Carg07221 vs. ExPASy TrEMBL
Match: A0A0A0K5S4 (LEC14B homolog OS=Cucumis sativus OX=3659 GN=Csa_7G391250 PE=3 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 5.9e-67
Identity = 118/123 (95.93%), Postives = 120/123 (97.56%), Query Frame = 0


Query: 1   MDYPLHAKNLMHPRDRSVATYKGHSVLRTLIRCYFSPESSTGQKYIYTGSHNSCVYIYDL 60
           MDYP HAKNLMHPRDRS+ATYKGHSVLRTLIRCYFSPE STGQKYIYTGSHNSCVYIYDL
Sbjct: 366 MDYPPHAKNLMHPRDRSLATYKGHSVLRTLIRCYFSPEYSTGQKYIYTGSHNSCVYIYDL 425

Query: 61  LTGALVATLKHHKSPVRDCSWHPQYPMLVSSSWDGDVVKWEFPGSGEAPTPPNKKRVRRR 120
           LTG LVATLKHHKSPVRDCSWHPQYPMLVSSSWDGDVVKWEFPGSGEAPTPPNKKRVRRR
Sbjct: 426 LTGVLVATLKHHKSPVRDCSWHPQYPMLVSSSWDGDVVKWEFPGSGEAPTPPNKKRVRRR 485

Query: 121 HYY 124
           H+Y
Sbjct: 486 HFY 488

[Close]

BLAST of Carg07221 vs. ExPASy TrEMBL
Match: A0A1S3BRH2 (LEC14B homolog OS=Cucumis melo OX=3656 GN=LOC103492712 PE=3 SV=1)

HSP 1 Score: 261.9 bits (668), Expect = 1.3e-66
Identity = 118/123 (95.93%), Postives = 119/123 (96.75%), Query Frame = 0


Query: 1   MDYPLHAKNLMHPRDRSVATYKGHSVLRTLIRCYFSPESSTGQKYIYTGSHNSCVYIYDL 60
           MDYP HAKNLMHP DRSVATYKGHSVLRTLIRCYFSPE STGQKYIYTGSHNSCVYIYDL
Sbjct: 366 MDYPPHAKNLMHPTDRSVATYKGHSVLRTLIRCYFSPEYSTGQKYIYTGSHNSCVYIYDL 425

Query: 61  LTGALVATLKHHKSPVRDCSWHPQYPMLVSSSWDGDVVKWEFPGSGEAPTPPNKKRVRRR 120
           LTG LVATLKHHKSPVRDCSWHPQYPMLVSSSWDGDVVKWEFPGSGEAPTPPNKKRVRRR
Sbjct: 426 LTGVLVATLKHHKSPVRDCSWHPQYPMLVSSSWDGDVVKWEFPGSGEAPTPPNKKRVRRR 485

Query: 121 HYY 124
           H+Y
Sbjct: 486 HFY 488

[Close]

BLAST of Carg07221 vs. TAIR 10
Match: AT4G03020.1 (transducin family protein / WD-40 repeat family protein )

HSP 1 Score: 208.8 bits (530), Expect = 2.5e-54
Identity = 94/123 (76.42%), Postives = 107/123 (86.99%), Query Frame = 0


Query: 1   MDYPLHAKNLMHPRDRSVATYKGHSVLRTLIRCYFSPESSTGQKYIYTGSHNSCVYIYDL 60
           MDYP  A++L HP D+SV+TYKGHSVLRTLIRCYFSP  STGQKYIYTGS++S VYIYDL
Sbjct: 371 MDYPTEARDLKHPLDQSVSTYKGHSVLRTLIRCYFSPAHSTGQKYIYTGSNDSSVYIYDL 430

Query: 61  LTGALVATLKHHKSPVRDCSWHPQYPMLVSSSWDGDVVKWEFPGSGEAPTPPNKKRVRRR 120
           ++G  VA LKHH SPVRDC+WHP YP L+SSSWDGD+VKWEFPGSGEAP   +KKRVRRR
Sbjct: 431 VSGDKVAVLKHHSSPVRDCNWHPYYPTLISSSWDGDLVKWEFPGSGEAPI-MSKKRVRRR 490

Query: 121 HYY 124
           H+Y
Sbjct: 491 HFY 492

[Close]

BLAST of Carg07221 vs. TAIR 10
Match: AT4G03020.2 (transducin family protein / WD-40 repeat family protein )

HSP 1 Score: 208.8 bits (530), Expect = 2.5e-54
Identity = 94/123 (76.42%), Postives = 107/123 (86.99%), Query Frame = 0


Query: 1   MDYPLHAKNLMHPRDRSVATYKGHSVLRTLIRCYFSPESSTGQKYIYTGSHNSCVYIYDL 60
           MDYP  A++L HP D+SV+TYKGHSVLRTLIRCYFSP  STGQKYIYTGS++S VYIYDL
Sbjct: 371 MDYPTEARDLKHPLDQSVSTYKGHSVLRTLIRCYFSPAHSTGQKYIYTGSNDSSVYIYDL 430

Query: 61  LTGALVATLKHHKSPVRDCSWHPQYPMLVSSSWDGDVVKWEFPGSGEAPTPPNKKRVRRR 120
           ++G  VA LKHH SPVRDC+WHP YP L+SSSWDGD+VKWEFPGSGEAP   +KKRVRRR
Sbjct: 431 VSGDKVAVLKHHSSPVRDCNWHPYYPTLISSSWDGDLVKWEFPGSGEAPI-MSKKRVRRR 490

Query: 121 HYY 124
           H+Y
Sbjct: 491 HFY 492

[Close]

BLAST of Carg07221 vs. TAIR 10
Match: AT5G08560.1 (transducin family protein / WD-40 repeat family protein )

HSP 1 Score: 53.5 bits (127), Expect = 1.4e-07
Identity = 33/84 (39.29%), Postives = 42/84 (50.00%), Query Frame = 0


Query: 18  VATYKGHSVLRTLIRCYFSPESSTGQKYIYTGSHNSCVYIYDLLTGALVATLKHHKSPVR 77
           V+ YKGH   R +IR  F       Q +I +GS +S VYI+   TG L+  L  H   V 
Sbjct: 479 VSRYKGHKRSRFIIRSCF---GGYKQAFIASGSEDSQVYIWHRSTGKLIVELPGHAGAVN 538

Query: 78  DCSWHP-QYPMLVSSSWDGDVVKW 101
             SW P    ML S+S DG +  W
Sbjct: 539 CVSWSPTNLHMLASASDDGTIRIW 559

[Close]

BLAST of Carg07221 vs. TAIR 10
Match: AT5G08560.2 (transducin family protein / WD-40 repeat family protein )

HSP 1 Score: 53.5 bits (127), Expect = 1.4e-07
Identity = 33/84 (39.29%), Postives = 42/84 (50.00%), Query Frame = 0


Query: 18  VATYKGHSVLRTLIRCYFSPESSTGQKYIYTGSHNSCVYIYDLLTGALVATLKHHKSPVR 77
           V+ YKGH   R +IR  F       Q +I +GS +S VYI+   TG L+  L  H   V 
Sbjct: 479 VSRYKGHKRSRFIIRSCF---GGYKQAFIASGSEDSQVYIWHRSTGKLIVELPGHAGAVN 538

Query: 78  DCSWHP-QYPMLVSSSWDGDVVKW 101
             SW P    ML S+S DG +  W
Sbjct: 539 CVSWSPTNLHMLASASDDGTIRIW 559

[Close]

BLAST of Carg07221 vs. TAIR 10
Match: AT1G11160.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 50.8 bits (120), Expect = 8.9e-07
Identity = 26/84 (30.95%), Postives = 45/84 (53.57%), Query Frame = 0


Query: 18  VATYKGHSVLRTLIRCYFSPESSTGQKYIYTGSHNSCVYIYDLLTGALVATLKHHKSPVR 77
           + TYKGH+  R +    FSP+     +++ +G  ++ V ++DL  G L+   K H+ P+R
Sbjct: 135 IQTYKGHT--RGISTIEFSPDG----RWVVSGGLDNVVKVWDLTAGKLLHEFKCHEGPIR 194

Query: 78  DCSWHPQYPMLVSSSWDGDVVKWE 102
              +HP   +L + S D  V  W+
Sbjct: 195 SLDFHPLEFLLATGSADRTVKFWD 212

The following BLAST results are available for this feature:

BLAST of Carg07221 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022954198.1	2.0e-69	100.00	LEC14B protein [Cucurbita moschata] >XP_022954199.1 LEC14B protein [Cucurbita mo...	[more]
KAG7014412.1	2.0e-69	100.00	hypothetical protein SDJN02_24589, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_023522133.1	5.9e-69	99.19	LEC14B protein-like [Cucurbita pepo subsp. pepo] >XP_023522135.1 LEC14B protein-...	[more]
XP_023548494.1	5.9e-69	99.19	LEC14B homolog [Cucurbita pepo subsp. pepo]	[more]
XP_022992516.1	2.9e-68	98.37	LEC14B protein [Cucurbita maxima] >XP_022992517.1 LEC14B protein [Cucurbita maxi...	[more]

BLAST of Carg07221 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O24467	5.0e-47	69.17	LEC14B homolog OS=Prunus armeniaca OX=36596 PE=2 SV=1	[more]
Q40153	4.7e-45	68.07	LEC14B protein OS=Lithospermum erythrorhizon OX=34254 PE=2 SV=1	[more]
Q91VU6	1.6e-24	53.68	DDB1- and CUL4-associated factor 11 OS=Mus musculus OX=10090 GN=Dcaf11 PE=1 SV=1	[more]
Q5M9G8	2.1e-24	53.68	DDB1- and CUL4-associated factor 11 OS=Rattus norvegicus OX=10116 GN=Dcaf11 PE=1...	[more]
Q5E9I8	6.0e-24	47.83	DDB1- and CUL4-associated factor 11 OS=Bos taurus OX=9913 GN=DCAF11 PE=2 SV=1	[more]

BLAST of Carg07221 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GQE7	9.8e-70	100.00	LEC14B homolog OS=Cucurbita moschata OX=3662 GN=LOC111456529 PE=3 SV=1	[more]
A0A6J1JQ33	1.4e-68	98.37	LEC14B homolog OS=Cucurbita maxima OX=3661 GN=LOC111488821 PE=3 SV=1	[more]
A0A5D3D413	2.7e-67	96.75	LEC14B homolog OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G00217...	[more]
A0A0A0K5S4	5.9e-67	95.93	LEC14B homolog OS=Cucumis sativus OX=3659 GN=Csa_7G391250 PE=3 SV=1	[more]
A0A1S3BRH2	1.3e-66	95.93	LEC14B homolog OS=Cucumis melo OX=3656 GN=LOC103492712 PE=3 SV=1	[more]

BLAST of Carg07221 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G03020.1	2.5e-54	76.42	transducin family protein / WD-40 repeat family protein	[more]
AT4G03020.2	2.5e-54	76.42	transducin family protein / WD-40 repeat family protein	[more]
AT5G08560.1	1.4e-07	39.29	transducin family protein / WD-40 repeat family protein	[more]
AT5G08560.2	1.4e-07	39.29	transducin family protein / WD-40 repeat family protein	[more]
AT1G11160.1	8.9e-07	30.95	Transducin/WD40 repeat-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001680	WD40 repeat	SMART	SM00320	WD40_4	coord: 62..101 e-value: 2.1E-7 score: 40.6 coord: 14..59 e-value: 0.65 score: 18.2
IPR001680	WD40 repeat	PFAM	PF00400	WD40	coord: 65..100 e-value: 1.1E-5 score: 26.1 coord: 18..59 e-value: 0.14 score: 13.1
IPR001680	WD40 repeat	PROSITE	PS50082	WD_REPEATS_2	coord: 69..101 score: 10.274523
IPR015943	WD40/YVTN repeat-like-containing domain superfamily	GENE3D	2.130.10.10		coord: 10..119 e-value: 5.6E-18 score: 67.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 103..123
None	No IPR available	PANTHER	PTHR19847:SF23	TRANSDUCIN/WD-LIKE REPEAT-PROTEIN	coord: 1..122
None	No IPR available	PANTHER	PTHR19847	DDB1- AND CUL4-ASSOCIATED FACTOR 11	coord: 1..122
None	No IPR available	PROSITE	PS50294	WD_REPEATS_REGION	coord: 69..101 score: 8.65015
IPR036322	WD40-repeat-containing domain superfamily	SUPERFAMILY	50978	WD40 repeat-like	coord: 15..103

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg07221-RA	Carg07221-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process
cellular_component	GO:0080008	Cul4-RING E3 ubiquitin ligase complex
molecular_function	GO:0005515	protein binding