Carg07217 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg07217
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionHistone H2A
LocationCarg_Chr17: 8360481 .. 8362422 (-)
RNA-Seq ExpressionCarg07217
SyntenyCarg07217
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTTCCACCGATGTTCCGACTAAGGGCGGCAGAGGCAAGAAATCTGCCGCCAAGTCAGTCTCCAGGTCTCACAAGGCTGGTCTTCAATTCCCCGTGGGTCGTATTGCCAGGTACCTCAAGAAGGGCCGTTATGCTCAGCGCGTAGGTTCTGGATCACCGGTTTACCTCTCCGCCGTCCTCGAATACCTCGCTGCCGAGGTTTGTAAGGCTAATTTCGTTTACAGTTTAATTCTTCGATTTTTAGTGTTTTGTTTCAAGAATTTTGCTTTTCGTTTAGGTTTTGGAGTTGGCTGGCAATGCTGCGAGGGACAACAAGAAGAACAGAATCATTCCGAGGCATATTCAGCTTGCGGTGAGGAACGATGAGGAATTGAGCAAGCTTTTGGGATCGGTTACGATTGCTAGTGGAGGAGTGATGCCGAAGATTCCTCAGTCTCTTTTGCCGAAGAAGGCTGGGAATGCGAAAGACAAGGCCGAAATTGGATCGGCATCTCAAGAATTTTAGGTTACCTTAGAAATCACTACCATTGATGATGAAATGAAAATTAAATAGTTAGGTTATGTTAGATTGTTTTAGCTTCAATTGATCATACTTCTTTCCGTAGGTAAATTTGTGAAATGAATTTTGCTTAGATCAGTTTTCGTCATTTTCGTCGTTCAACTTTTTTATTGTGTAAATGCTGATGAAGTTCACGGCTAATTAGAATCGAAAGAAACTTATGGAGTTTTCAATTTCCTGGAATTAGTAAATTTTCTTGCATGCCAATAAAAAATTACGAGATATAACCGCTTTAAAATGCGAAATCTTCAATTTACTTAATTGGCCACTAATTTAAATGTTTATTTTTTTTCATTAATGATATGGAAGTTGCTGATTCATGTGATTTCGTTTCTCTCTCTCTATATATTTTAGGGATCCTTCTTCCTTAATCCCTCGATTTTTCTTTTTTCGATTCTATCTCTATCAAGTTGCATATTATCTAGGGTTTGATTATCCGCCGGGAAAAACTATACTATGAGTTCATCGGCTTCGACCAAGGGCGGCAGAGGGAAACCAAAGGCCACGAAGTCTGTTTCGCGGTCTCAGAAGGCTGGTCTTCAGTTTCCCGTCGGTCGTATTGCGAGGTTTCTTAAGGCCGGTAAGTATGCGGAGCGCGTTGGCGCCGGTGCTCCCGTCTACCTCTCCGCTGTCCTTGAATATCTGGCTGCCGAGGTTAGTGCTTGCTCTTCTCATCGGATCTTTTACTTGTGTTTCCGATCTGTTTATCTGTGGAGTTGTTTTCGTCTGATTGTGATGAAGTGAGGTGCTGTAGTGTAGTGCTTTGATCACTTGAATCGAAATTCTTATATTTGATTTTGTTGGGTTTCGTCTGCTTCGGCTCTGGAATTGATTTGGAGATGATGAATTTCGATTTGTTTCTTTAAATTGATGAAGAAACTATTTGAAATTAGGTTCACCATTGCATAGTAATCTCGAGCTTTGAATTGTGGCCTCCCTTTTTCTCTTGCATGTCATCCAATTGGCTAGAAATTGATACTGAATGCAGCTTATTTAGTTTTACTTGGTGAATAAGATGCCAAAGCCACAAGACTATGTTAGTTTGTTCTTTGGATGAACGAAGTTTCATTTGGGTTCATCCCTATTGTTTCTGTACAATGATGATGAATTGGTTTTGCATTTAAAGTAGCTGAATATACTAAAAATCTTCATTTGCAGGTGCTTGAGCTTGCAGGCAATGCTGCCAGAGACAACAAGAAGAACCGTATTGTTCCGAGGCACATACAGCTCGCAGTCCGTAACGACGAAGAGCTAAGCAAGCTTTTGGGGGATGTGACCATTGCAAATGGTGGAGTGTTGCCGAATATCCATCAAAATCTCTTGCCTAAGAAAGCCGGGTCGGGTAAAGGCGACATTGGATCTGCATCTCAAGAGTTCTAA

mRNA sequence

ATGAGTTCCACCGATGTTCCGACTAAGGGCGGCAGAGGCAAGAAATCTGCCGCCAAGTCAGTCTCCAGGTCTCACAAGGCTGGTCTTCAATTCCCCGTGGGTCGTATTGCCAGGTACCTCAAGAAGGGCCGTTATGCTCAGCGCGTAGGTTCTGGATCACCGGTTTACCTCTCCGCCGTCCTCGAATACCTCGCTGCCGAGGGCGGCAGAGGGAAACCAAAGGCCACGAAGTCTGTTTCGCGGTCTCAGAAGGCTGGTCTTCAGTTTCCCGTCGGTCGTATTGCGAGGTTTCTTAAGGCCGGTAAGTATGCGGAGCGCGTTGGCGCCGGTGCTCCCGTCTACCTCTCCGCTGTCCTTGAATATCTGGCTGCCGAGGTGCTTGAGCTTGCAGGCAATGCTGCCAGAGACAACAAGAAGAACCGTATTGTTCCGAGGCACATACAGCTCGCAGTCCGTAACGACGAAGAGCTAAGCAAGCTTTTGGGGGATGTGACCATTGCAAATGGTGGAGTGTTGCCGAATATCCATCAAAATCTCTTGCCTAAGAAAGCCGGGTCGGGTAAAGGCGACATTGGATCTGCATCTCAAGAGTTCTAA

Coding sequence (CDS)

ATGAGTTCCACCGATGTTCCGACTAAGGGCGGCAGAGGCAAGAAATCTGCCGCCAAGTCAGTCTCCAGGTCTCACAAGGCTGGTCTTCAATTCCCCGTGGGTCGTATTGCCAGGTACCTCAAGAAGGGCCGTTATGCTCAGCGCGTAGGTTCTGGATCACCGGTTTACCTCTCCGCCGTCCTCGAATACCTCGCTGCCGAGGGCGGCAGAGGGAAACCAAAGGCCACGAAGTCTGTTTCGCGGTCTCAGAAGGCTGGTCTTCAGTTTCCCGTCGGTCGTATTGCGAGGTTTCTTAAGGCCGGTAAGTATGCGGAGCGCGTTGGCGCCGGTGCTCCCGTCTACCTCTCCGCTGTCCTTGAATATCTGGCTGCCGAGGTGCTTGAGCTTGCAGGCAATGCTGCCAGAGACAACAAGAAGAACCGTATTGTTCCGAGGCACATACAGCTCGCAGTCCGTAACGACGAAGAGCTAAGCAAGCTTTTGGGGGATGTGACCATTGCAAATGGTGGAGTGTTGCCGAATATCCATCAAAATCTCTTGCCTAAGAAAGCCGGGTCGGGTAAAGGCGACATTGGATCTGCATCTCAAGAGTTCTAA

Protein sequence

MSSTDVPTKGGRGKKSAAKSVSRSHKAGLQFPVGRIARYLKKGRYAQRVGSGSPVYLSAVLEYLAAEGGRGKPKATKSVSRSQKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLSAVLEYLAAEVLELAGNAARDNKKNRIVPRHIQLAVRNDEELSKLLGDVTIANGGVLPNIHQNLLPKKAGSGKGDIGSASQEF
Homology
BLAST of Carg07217 vs. NCBI nr
Match: KAG7014408.1 (Histone H2AX, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 378.6 bits (971), Expect = 3.2e-101
Identity = 198/198 (100.00%), Postives = 198/198 (100.00%), Query Frame = 0

Query: 1   MSSTDVPTKGGRGKKSAAKSVSRSHKAGLQFPVGRIARYLKKGRYAQRVGSGSPVYLSAV 60
           MSSTDVPTKGGRGKKSAAKSVSRSHKAGLQFPVGRIARYLKKGRYAQRVGSGSPVYLSAV
Sbjct: 1   MSSTDVPTKGGRGKKSAAKSVSRSHKAGLQFPVGRIARYLKKGRYAQRVGSGSPVYLSAV 60

Query: 61  LEYLAAEGGRGKPKATKSVSRSQKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLSAVLE 120
           LEYLAAEGGRGKPKATKSVSRSQKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLSAVLE
Sbjct: 61  LEYLAAEGGRGKPKATKSVSRSQKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLSAVLE 120

Query: 121 YLAAEVLELAGNAARDNKKNRIVPRHIQLAVRNDEELSKLLGDVTIANGGVLPNIHQNLL 180
           YLAAEVLELAGNAARDNKKNRIVPRHIQLAVRNDEELSKLLGDVTIANGGVLPNIHQNLL
Sbjct: 121 YLAAEVLELAGNAARDNKKNRIVPRHIQLAVRNDEELSKLLGDVTIANGGVLPNIHQNLL 180

Query: 181 PKKAGSGKGDIGSASQEF 199
           PKKAGSGKGDIGSASQEF
Sbjct: 181 PKKAGSGKGDIGSASQEF 198

BLAST of Carg07217 vs. NCBI nr
Match: KAG5514359.1 (hypothetical protein RHGRI_035685 [Rhododendron griersonianum])

HSP 1 Score: 313.5 bits (802), Expect = 1.3e-81
Identity = 175/247 (70.85%), Postives = 183/247 (74.09%), Query Frame = 0

Query: 2   SSTDVPTKGGRGKKSAAKSVSRSHKAGLQFPVGRIARYLKKGRYAQRVGSGSPVYLSAVL 61
           S+    T GGRGK    K+VSRS KAGLQFPVGR+AR+L+KGRYAQRVGSGSP+YLSAVL
Sbjct: 3   STAPATTTGGRGKARGTKAVSRSLKAGLQFPVGRVARFLRKGRYAQRVGSGSPIYLSAVL 62

Query: 62  EYLAAE--------------------------------------------------GGRG 121
           EYLAAE                                                  GGRG
Sbjct: 63  EYLAAEVLELAGNAARDNKKNRIAPRHIQLAVRNDEEFSKLVFDQPHTMSSTAASKGGRG 122

Query: 122 KPKATKSVSRSQKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLSAVLEYLAAEVLELAG 181
           KPKA+KSVSRS KAGLQFPVGRIARFLKAGKYAERVGAGAPVYLSAVLEYLAAEVLELAG
Sbjct: 123 KPKASKSVSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLSAVLEYLAAEVLELAG 182

Query: 182 NAARDNKKNRIVPRHIQLAVRNDEELSKLLGDVTIANGGVLPNIHQNLLPKKAGSGKGDI 199
           NAARDNKKNRIVPRHIQLAVRNDEELSKLLG VTIANGGVLPNIHQNLLPKK G GKG+I
Sbjct: 183 NAARDNKKNRIVPRHIQLAVRNDEELSKLLGTVTIANGGVLPNIHQNLLPKKVGKGKGEI 242

BLAST of Carg07217 vs. NCBI nr
Match: KDP21535.1 (hypothetical protein JCGZ_22006 [Jatropha curcas])

HSP 1 Score: 307.4 bits (786), Expect = 9.1e-80
Identity = 183/284 (64.44%), Postives = 191/284 (67.25%), Query Frame = 0

Query: 1   MSSTDVPT------KGGRGKKSAAKSVSRSHKAGLQFPVGRIARYLKKGRYAQRVGSGSP 60
           MSST VP+      +GGRG  S AKSVSRSHKAGLQFPVGR+AR+LK+GRYAQRVGSGSP
Sbjct: 1   MSSTTVPSATAKTARGGRG-ISKAKSVSRSHKAGLQFPVGRVARFLKRGRYAQRVGSGSP 60

Query: 61  VYLSAVLEYLAAE----------------------------------------------- 120
           VYLSAVLEYLAAE                                               
Sbjct: 61  VYLSAVLEYLAAEVLELAGNAARDNKKNRIIPRHIQLAVRNDEELSKLLGSVTIANGGVL 120

Query: 121 ---------------------------------GGRGKPKATKSVSRSQKAGLQFPVGRI 180
                                            GGRGKPK++KSVSRSQKAGLQFPVGRI
Sbjct: 121 PNIHQNLLPKKTAKGKGLGINRKPAMSSGGSTKGGRGKPKSSKSVSRSQKAGLQFPVGRI 180

Query: 181 ARFLKAGKYAERVGAGAPVYLSAVLEYLAAEVLELAGNAARDNKKNRIVPRHIQLAVRND 199
           ARFLKAGKYA+RVGAGAPVYLSAVLEYLAAEVLELAGNAARDNKKNRIVPRHIQLAVRND
Sbjct: 181 ARFLKAGKYADRVGAGAPVYLSAVLEYLAAEVLELAGNAARDNKKNRIVPRHIQLAVRND 240

BLAST of Carg07217 vs. NCBI nr
Match: GAY40811.1 (hypothetical protein CUMW_054740 [Citrus unshiu])

HSP 1 Score: 298.5 bits (763), Expect = 4.2e-77
Identity = 176/295 (59.66%), Postives = 184/295 (62.37%), Query Frame = 0

Query: 1   MSSTDVPTKGGRGKKSAAKSVSRSHKAGLQFPVGRIARYLKKGRYAQRVGSGSPVYLSAV 60
           MSS    TKGGRG+    K VSRSHKAGLQFPVGR+AR+LKKGRYAQRVGSGSPVYLSAV
Sbjct: 1   MSSEAAATKGGRGRSKDTKPVSRSHKAGLQFPVGRVARFLKKGRYAQRVGSGSPVYLSAV 60

Query: 61  LEYLAAE----------------------------------------------------- 120
           LEYLAAE                                                     
Sbjct: 61  LEYLAAEVLELAGNAARDNKKNRIIPRHIQLAVKNDEEFSKLLGSVTIANGGVLPNIHQN 120

Query: 121 --------------------------------------------GGRGKPKATKSVSRSQ 180
                                                       GGRGKPK+TKS+SRS 
Sbjct: 121 LLPKKAAARKGEIGSVSQEASPESASKRAFALFCQSMSSAGSTKGGRGKPKSTKSISRSH 180

Query: 181 KAGLQFPVGRIARFLKAGKYAERVGAGAPVYLSAVLEYLAAEVLELAGNAARDNKKNRIV 199
           KAGLQFPVGRIARFLKAGKYA+RVGAGAPVYLSAVLEYLAAEVLELAGNAARDNKKNRIV
Sbjct: 181 KAGLQFPVGRIARFLKAGKYADRVGAGAPVYLSAVLEYLAAEVLELAGNAARDNKKNRIV 240

BLAST of Carg07217 vs. NCBI nr
Match: KAF4368296.1 (hypothetical protein G4B88_008600 [Cannabis sativa])

HSP 1 Score: 295.0 bits (754), Expect = 4.7e-76
Identity = 179/293 (61.09%), Postives = 183/293 (62.46%), Query Frame = 0

Query: 1   MSSTDVPT--KGGRGKKSAAKSVSRSHKAGLQFPVGRIARYLKKGRYAQRVGSGSPVYLS 60
           M ST   T  KGGRGK  A KSVSRS KAGLQFPVGRIAR+LKKGRYAQRVGSGSPVYLS
Sbjct: 536 MDSTAAATTKKGGRGKPKANKSVSRSSKAGLQFPVGRIARFLKKGRYAQRVGSGSPVYLS 595

Query: 61  AVLEYLAAE--------------------------------------------------- 120
           AVLEYLAAE                                                   
Sbjct: 596 AVLEYLAAELLELAGNAARDNKKSRIIPRHIQLAVRNDEELSKLLGAVTIANGGVLPNIH 655

Query: 121 ------------------------------------------GGRGKPKATKSVSRSQKA 180
                                                     GGRGKPK++KSVSRS KA
Sbjct: 656 QTLLPKKVGKGKDEIGSASQEVYLYFDPDSKTLKMSTAATTKGGRGKPKSSKSVSRSSKA 715

Query: 181 GLQFPVGRIARFLKAGKYAERVGAGAPVYLSAVLEYLAAEVLELAGNAARDNKKNRIVPR 199
           GLQFPVGRIARFLK GKYAERVGAGAPVYLSAVLEYLAAEVLELAGNAARDNKKNRIVPR
Sbjct: 716 GLQFPVGRIARFLKNGKYAERVGAGAPVYLSAVLEYLAAEVLELAGNAARDNKKNRIVPR 775

BLAST of Carg07217 vs. ExPASy Swiss-Prot
Match: O65759 (Histone H2AX OS=Cicer arietinum OX=3827 GN=HIS2A PE=2 SV=1)

HSP 1 Score: 239.6 bits (610), Expect = 3.1e-62
Identity = 123/132 (93.18%), Postives = 126/132 (95.45%), Query Frame = 0

Query: 67  EGGRGKPKATKSVSRSQKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLSAVLEYLAAEV 126
           +GGRGKPKA+KSVSRS KAGLQFPVGRIARFLKAGKYAERVGAGAPVYLSAVLEYLAAEV
Sbjct: 8   KGGRGKPKASKSVSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLSAVLEYLAAEV 67

Query: 127 LELAGNAARDNKKNRIVPRHIQLAVRNDEELSKLLGDVTIANGGVLPNIHQNLLPKKAGS 186
           LELAGNAARDNK NRIVPRHIQLAVRNDEELSKLLG VTIANGGVLPNIHQ LLPKK G 
Sbjct: 68  LELAGNAARDNKNNRIVPRHIQLAVRNDEELSKLLGSVTIANGGVLPNIHQTLLPKKVGK 127

Query: 187 GKGDIGSASQEF 199
           GKG+IGSASQEF
Sbjct: 128 GKGEIGSASQEF 139

BLAST of Carg07217 vs. ExPASy Swiss-Prot
Match: Q9S9K7 (Probable histone H2AXb OS=Arabidopsis thaliana OX=3702 GN=At1g54690 PE=1 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 8.9e-62
Identity = 123/132 (93.18%), Postives = 124/132 (93.94%), Query Frame = 0

Query: 67  EGGRGKPKATKSVSRSQKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLSAVLEYLAAEV 126
           +GGRGKPKATKSVSRS KAGLQFPVGRIARFLKAGKYAERVGAGAPVYLSAVLEYLAAEV
Sbjct: 11  KGGRGKPKATKSVSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLSAVLEYLAAEV 70

Query: 127 LELAGNAARDNKKNRIVPRHIQLAVRNDEELSKLLGDVTIANGGVLPNIHQNLLPKKAGS 186
           LELAGNAARDNKK RIVPRHIQLAVRNDEELSKLLG VTIANGGVLPNIHQ LLP K G 
Sbjct: 71  LELAGNAARDNKKTRIVPRHIQLAVRNDEELSKLLGSVTIANGGVLPNIHQTLLPSKVGK 130

Query: 187 GKGDIGSASQEF 199
            KGDIGSASQEF
Sbjct: 131 NKGDIGSASQEF 142

BLAST of Carg07217 vs. ExPASy Swiss-Prot
Match: O04848 (Probable histone H2AXa OS=Arabidopsis thaliana OX=3702 GN=At1g08880 PE=1 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 2.0e-61
Identity = 122/132 (92.42%), Postives = 124/132 (93.94%), Query Frame = 0

Query: 67  EGGRGKPKATKSVSRSQKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLSAVLEYLAAEV 126
           +GGRGKPKATKSVSRS KAGLQFPVGRIARFLK+GKYAERVGAGAPVYLSAVLEYLAAEV
Sbjct: 11  KGGRGKPKATKSVSRSSKAGLQFPVGRIARFLKSGKYAERVGAGAPVYLSAVLEYLAAEV 70

Query: 127 LELAGNAARDNKKNRIVPRHIQLAVRNDEELSKLLGDVTIANGGVLPNIHQNLLPKKAGS 186
           LELAGNAARDNKK RIVPRHIQLAVRNDEELSKLLG VTIANGGVLPNIHQ LLP K G 
Sbjct: 71  LELAGNAARDNKKTRIVPRHIQLAVRNDEELSKLLGSVTIANGGVLPNIHQTLLPSKVGK 130

Query: 187 GKGDIGSASQEF 199
            KGDIGSASQEF
Sbjct: 131 NKGDIGSASQEF 142

BLAST of Carg07217 vs. ExPASy Swiss-Prot
Match: P35063 (Histone H2AX OS=Picea abies OX=3329 PE=2 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 2.0e-61
Identity = 123/133 (92.48%), Postives = 128/133 (96.24%), Query Frame = 0

Query: 66  AEGGRGKPKATKSVSRSQKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLSAVLEYLAAE 125
           A+GGRGK K+TKSVSRSQKAGLQFPVGRIARFLKAGKYAERVGAGAPVYL+AVLEYLAAE
Sbjct: 6   AKGGRGKAKSTKSVSRSQKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLAAVLEYLAAE 65

Query: 126 VLELAGNAARDNKKNRIVPRHIQLAVRNDEELSKLLGDVTIANGGVLPNIHQNLLPKKAG 185
           VLELAGNAARDNKKNRIVPRHIQLAVRNDEELSKLLG VTIANGGVLPNIHQ LLPKK+G
Sbjct: 66  VLELAGNAARDNKKNRIVPRHIQLAVRNDEELSKLLGAVTIANGGVLPNIHQVLLPKKSG 125

Query: 186 SGKGDIGSASQEF 199
             KG+IGSASQEF
Sbjct: 126 KDKGEIGSASQEF 138

BLAST of Carg07217 vs. ExPASy Swiss-Prot
Match: A2ZL69 (Probable histone H2AXb OS=Oryza sativa subsp. indica OX=39946 GN=OsI_037312 PE=3 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 2.1e-58
Identity = 118/131 (90.08%), Postives = 121/131 (92.37%), Query Frame = 0

Query: 68  GGRGKPKATKSVSRSQKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLSAVLEYLAAEVL 127
           GGRGK K +KSVSRS KAGLQFPVGRIAR+LKAGKYAERVGAGAPVYLSAVLEYLAAEVL
Sbjct: 8   GGRGKSKGSKSVSRSSKAGLQFPVGRIARYLKAGKYAERVGAGAPVYLSAVLEYLAAEVL 67

Query: 128 ELAGNAARDNKKNRIVPRHIQLAVRNDEELSKLLGDVTIANGGVLPNIHQNLLPKKAGSG 187
           ELAGNAARDNKKNRIVPRHIQLAVRNDEELS+LLG VTIA GGVLPNIHQ LLPKK G  
Sbjct: 68  ELAGNAARDNKKNRIVPRHIQLAVRNDEELSRLLGAVTIAAGGVLPNIHQTLLPKKGGKD 127

Query: 188 KGDIGSASQEF 199
           K DIGSASQEF
Sbjct: 128 KADIGSASQEF 138

BLAST of Carg07217 vs. ExPASy TrEMBL
Match: A0A067JNE6 (Histone H2A OS=Jatropha curcas OX=180498 GN=JCGZ_22006 PE=3 SV=1)

HSP 1 Score: 307.4 bits (786), Expect = 4.4e-80
Identity = 183/284 (64.44%), Postives = 191/284 (67.25%), Query Frame = 0

Query: 1   MSSTDVPT------KGGRGKKSAAKSVSRSHKAGLQFPVGRIARYLKKGRYAQRVGSGSP 60
           MSST VP+      +GGRG  S AKSVSRSHKAGLQFPVGR+AR+LK+GRYAQRVGSGSP
Sbjct: 1   MSSTTVPSATAKTARGGRG-ISKAKSVSRSHKAGLQFPVGRVARFLKRGRYAQRVGSGSP 60

Query: 61  VYLSAVLEYLAAE----------------------------------------------- 120
           VYLSAVLEYLAAE                                               
Sbjct: 61  VYLSAVLEYLAAEVLELAGNAARDNKKNRIIPRHIQLAVRNDEELSKLLGSVTIANGGVL 120

Query: 121 ---------------------------------GGRGKPKATKSVSRSQKAGLQFPVGRI 180
                                            GGRGKPK++KSVSRSQKAGLQFPVGRI
Sbjct: 121 PNIHQNLLPKKTAKGKGLGINRKPAMSSGGSTKGGRGKPKSSKSVSRSQKAGLQFPVGRI 180

Query: 181 ARFLKAGKYAERVGAGAPVYLSAVLEYLAAEVLELAGNAARDNKKNRIVPRHIQLAVRND 199
           ARFLKAGKYA+RVGAGAPVYLSAVLEYLAAEVLELAGNAARDNKKNRIVPRHIQLAVRND
Sbjct: 181 ARFLKAGKYADRVGAGAPVYLSAVLEYLAAEVLELAGNAARDNKKNRIVPRHIQLAVRND 240

BLAST of Carg07217 vs. ExPASy TrEMBL
Match: A0A2H5NLL6 (Histone H2A OS=Citrus unshiu OX=55188 GN=CUMW_054740 PE=3 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 2.0e-77
Identity = 176/295 (59.66%), Postives = 184/295 (62.37%), Query Frame = 0

Query: 1   MSSTDVPTKGGRGKKSAAKSVSRSHKAGLQFPVGRIARYLKKGRYAQRVGSGSPVYLSAV 60
           MSS    TKGGRG+    K VSRSHKAGLQFPVGR+AR+LKKGRYAQRVGSGSPVYLSAV
Sbjct: 1   MSSEAAATKGGRGRSKDTKPVSRSHKAGLQFPVGRVARFLKKGRYAQRVGSGSPVYLSAV 60

Query: 61  LEYLAAE----------------------------------------------------- 120
           LEYLAAE                                                     
Sbjct: 61  LEYLAAEVLELAGNAARDNKKNRIIPRHIQLAVKNDEEFSKLLGSVTIANGGVLPNIHQN 120

Query: 121 --------------------------------------------GGRGKPKATKSVSRSQ 180
                                                       GGRGKPK+TKS+SRS 
Sbjct: 121 LLPKKAAARKGEIGSVSQEASPESASKRAFALFCQSMSSAGSTKGGRGKPKSTKSISRSH 180

Query: 181 KAGLQFPVGRIARFLKAGKYAERVGAGAPVYLSAVLEYLAAEVLELAGNAARDNKKNRIV 199
           KAGLQFPVGRIARFLKAGKYA+RVGAGAPVYLSAVLEYLAAEVLELAGNAARDNKKNRIV
Sbjct: 181 KAGLQFPVGRIARFLKAGKYADRVGAGAPVYLSAVLEYLAAEVLELAGNAARDNKKNRIV 240

BLAST of Carg07217 vs. ExPASy TrEMBL
Match: A0A7J6FC78 (Uncharacterized protein OS=Cannabis sativa OX=3483 GN=G4B88_008600 PE=3 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 2.3e-76
Identity = 179/293 (61.09%), Postives = 183/293 (62.46%), Query Frame = 0

Query: 1   MSSTDVPT--KGGRGKKSAAKSVSRSHKAGLQFPVGRIARYLKKGRYAQRVGSGSPVYLS 60
           M ST   T  KGGRGK  A KSVSRS KAGLQFPVGRIAR+LKKGRYAQRVGSGSPVYLS
Sbjct: 536 MDSTAAATTKKGGRGKPKANKSVSRSSKAGLQFPVGRIARFLKKGRYAQRVGSGSPVYLS 595

Query: 61  AVLEYLAAE--------------------------------------------------- 120
           AVLEYLAAE                                                   
Sbjct: 596 AVLEYLAAELLELAGNAARDNKKSRIIPRHIQLAVRNDEELSKLLGAVTIANGGVLPNIH 655

Query: 121 ------------------------------------------GGRGKPKATKSVSRSQKA 180
                                                     GGRGKPK++KSVSRS KA
Sbjct: 656 QTLLPKKVGKGKDEIGSASQEVYLYFDPDSKTLKMSTAATTKGGRGKPKSSKSVSRSSKA 715

Query: 181 GLQFPVGRIARFLKAGKYAERVGAGAPVYLSAVLEYLAAEVLELAGNAARDNKKNRIVPR 199
           GLQFPVGRIARFLK GKYAERVGAGAPVYLSAVLEYLAAEVLELAGNAARDNKKNRIVPR
Sbjct: 716 GLQFPVGRIARFLKNGKYAERVGAGAPVYLSAVLEYLAAEVLELAGNAARDNKKNRIVPR 775

BLAST of Carg07217 vs. ExPASy TrEMBL
Match: A0A7J6EH34 (Uncharacterized protein OS=Cannabis sativa OX=3483 GN=F8388_007218 PE=3 SV=1)

HSP 1 Score: 282.0 bits (720), Expect = 2.0e-72
Identity = 179/327 (54.74%), Postives = 183/327 (55.96%), Query Frame = 0

Query: 1   MSSTDVPT--KGGRGKKSAAKSVSRSHKAGLQFPVGRIARYLKKGRYAQRVGSGSPVYLS 60
           M ST   T  KGGRGK  A KSVSRS KAGLQFPVGRIAR+LKKGRYAQRVGSGSPVYLS
Sbjct: 80  MDSTAAATTKKGGRGKPKANKSVSRSSKAGLQFPVGRIARFLKKGRYAQRVGSGSPVYLS 139

Query: 61  AVLEYLAAE--------------------------------------------------- 120
           AVLEYLAAE                                                   
Sbjct: 140 AVLEYLAAELLELAGNAARDNKKSRIIPRHIQLAVRNDEELSKLLGAVTIANGGVLPNIH 199

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 200 QTLLPKKVGKGKDEIGSASQDLVEEVGLLCKHSLSLQYVSFSLSCRSSCSITFPRVYLYF 259

Query: 181 ----------------GGRGKPKATKSVSRSQKAGLQFPVGRIARFLKAGKYAERVGAGA 199
                           GGRGKPK++KSVSRS KAGLQFPVGRIARFLK GKYAERVGAGA
Sbjct: 260 DPDSKTLKMSTAATTKGGRGKPKSSKSVSRSSKAGLQFPVGRIARFLKNGKYAERVGAGA 319

BLAST of Carg07217 vs. ExPASy TrEMBL
Match: A0A150G9R4 (Histone H2A OS=Gonium pectorale OX=33097 GN=GPECTOR_42g800 PE=3 SV=1)

HSP 1 Score: 271.9 bits (694), Expect = 2.1e-69
Identity = 144/199 (72.36%), Postives = 163/199 (81.91%), Query Frame = 0

Query: 11  GRGKKSAAKSVSRSHKAGLQFPVGRIARYLKKGRYAQRVGSGSPVYLSAVLEYLAAE--- 70
           G+GK +  K+VSRS KAGLQFPVGRIARYLKKG+YA+R+G+G+PVYL+AVLEYL AE   
Sbjct: 5   GKGKTAGKKAVSRSSKAGLQFPVGRIARYLKKGKYAERIGAGAPVYLAAVLEYLTAEVLE 64

Query: 71  -----------GGRGKPKATKSVSRSQKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLS 130
                         GK    K+VSRS KAGLQFPVGRIAR+LK GKYAER+GAGAPVYL+
Sbjct: 65  LAGNAARDNKKNRIGKTAGKKAVSRSSKAGLQFPVGRIARYLKKGKYAERIGAGAPVYLA 124

Query: 131 AVLEYLAAEVLELAGNAARDNKKNRIVPRHIQLAVRNDEELSKLLGDVTIANGGVLPNIH 190
           AVLEYL AEVLELAGNAARDNKKNRIVPRHIQLA+RNDEEL KLLG+VTIA+GGVLPNIH
Sbjct: 125 AVLEYLTAEVLELAGNAARDNKKNRIVPRHIQLAIRNDEELGKLLGEVTIASGGVLPNIH 184

Query: 191 QNLLPKKAGSGKGDIGSAS 196
             LLPKK+  GK + GSA+
Sbjct: 185 AVLLPKKSKGGKAEDGSAA 203

BLAST of Carg07217 vs. TAIR 10
Match: AT1G54690.1 (gamma histone variant H2AX )

HSP 1 Score: 238.0 bits (606), Expect = 6.3e-63
Identity = 123/132 (93.18%), Postives = 124/132 (93.94%), Query Frame = 0

Query: 67  EGGRGKPKATKSVSRSQKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLSAVLEYLAAEV 126
           +GGRGKPKATKSVSRS KAGLQFPVGRIARFLKAGKYAERVGAGAPVYLSAVLEYLAAEV
Sbjct: 11  KGGRGKPKATKSVSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLSAVLEYLAAEV 70

Query: 127 LELAGNAARDNKKNRIVPRHIQLAVRNDEELSKLLGDVTIANGGVLPNIHQNLLPKKAGS 186
           LELAGNAARDNKK RIVPRHIQLAVRNDEELSKLLG VTIANGGVLPNIHQ LLP K G 
Sbjct: 71  LELAGNAARDNKKTRIVPRHIQLAVRNDEELSKLLGSVTIANGGVLPNIHQTLLPSKVGK 130

Query: 187 GKGDIGSASQEF 199
            KGDIGSASQEF
Sbjct: 131 NKGDIGSASQEF 142

BLAST of Carg07217 vs. TAIR 10
Match: AT1G08880.1 (Histone superfamily protein )

HSP 1 Score: 236.9 bits (603), Expect = 1.4e-62
Identity = 122/132 (92.42%), Postives = 124/132 (93.94%), Query Frame = 0

Query: 67  EGGRGKPKATKSVSRSQKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLSAVLEYLAAEV 126
           +GGRGKPKATKSVSRS KAGLQFPVGRIARFLK+GKYAERVGAGAPVYLSAVLEYLAAEV
Sbjct: 11  KGGRGKPKATKSVSRSSKAGLQFPVGRIARFLKSGKYAERVGAGAPVYLSAVLEYLAAEV 70

Query: 127 LELAGNAARDNKKNRIVPRHIQLAVRNDEELSKLLGDVTIANGGVLPNIHQNLLPKKAGS 186
           LELAGNAARDNKK RIVPRHIQLAVRNDEELSKLLG VTIANGGVLPNIHQ LLP K G 
Sbjct: 71  LELAGNAARDNKKTRIVPRHIQLAVRNDEELSKLLGSVTIANGGVLPNIHQTLLPSKVGK 130

Query: 187 GKGDIGSASQEF 199
            KGDIGSASQEF
Sbjct: 131 NKGDIGSASQEF 142

BLAST of Carg07217 vs. TAIR 10
Match: AT4G27230.1 (histone H2A 2 )

HSP 1 Score: 203.8 bits (517), Expect = 1.3e-52
Identity = 109/124 (87.90%), Postives = 111/124 (89.52%), Query Frame = 0

Query: 69  GRGKP----KATKSVSRSQKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLSAVLEYLAA 128
           GRGK      A KS SRS KAGLQFPVGRIARFLKAGKYAERVGAGAPVYL+AVLEYLAA
Sbjct: 3   GRGKQLGSGAAKKSTSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLAAVLEYLAA 62

Query: 129 EVLELAGNAARDNKKNRIVPRHIQLAVRNDEELSKLLGDVTIANGGVLPNIHQNLLPKKA 188
           EVLELAGNAARDNKK RIVPRHIQLAVRNDEELSKLLGDVTIANGGV+PNIH  LLPKKA
Sbjct: 63  EVLELAGNAARDNKKTRIVPRHIQLAVRNDEELSKLLGDVTIANGGVMPNIHNLLLPKKA 122

BLAST of Carg07217 vs. TAIR 10
Match: AT4G27230.2 (histone H2A 2 )

HSP 1 Score: 203.8 bits (517), Expect = 1.3e-52
Identity = 109/124 (87.90%), Postives = 111/124 (89.52%), Query Frame = 0

Query: 69  GRGKP----KATKSVSRSQKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLSAVLEYLAA 128
           GRGK      A KS SRS KAGLQFPVGRIARFLKAGKYAERVGAGAPVYL+AVLEYLAA
Sbjct: 3   GRGKQLGSGAAKKSTSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLAAVLEYLAA 62

Query: 129 EVLELAGNAARDNKKNRIVPRHIQLAVRNDEELSKLLGDVTIANGGVLPNIHQNLLPKKA 188
           EVLELAGNAARDNKK RIVPRHIQLAVRNDEELSKLLGDVTIANGGV+PNIH  LLPKKA
Sbjct: 63  EVLELAGNAARDNKKTRIVPRHIQLAVRNDEELSKLLGDVTIANGGVMPNIHNLLLPKKA 122

BLAST of Carg07217 vs. TAIR 10
Match: AT5G54640.1 (Histone superfamily protein )

HSP 1 Score: 201.1 bits (510), Expect = 8.6e-52
Identity = 107/124 (86.29%), Postives = 111/124 (89.52%), Query Frame = 0

Query: 69  GRGKP----KATKSVSRSQKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLSAVLEYLAA 128
           GRGK      A K+ SRS KAGLQFPVGRIARFLKAGKYAERVGAGAPVYL+AVLEYLAA
Sbjct: 3   GRGKTLGSGGAKKATSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLAAVLEYLAA 62

Query: 129 EVLELAGNAARDNKKNRIVPRHIQLAVRNDEELSKLLGDVTIANGGVLPNIHQNLLPKKA 188
           EVLELAGNAARDNKK RIVPRHIQLAVRNDEELSKLLGDVTIANGGV+PNIH  LLPKKA
Sbjct: 63  EVLELAGNAARDNKKTRIVPRHIQLAVRNDEELSKLLGDVTIANGGVMPNIHNLLLPKKA 122

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7014408.13.2e-101100.00Histone H2AX, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG5514359.11.3e-8170.85hypothetical protein RHGRI_035685 [Rhododendron griersonianum][more]
KDP21535.19.1e-8064.44hypothetical protein JCGZ_22006 [Jatropha curcas][more]
GAY40811.14.2e-7759.66hypothetical protein CUMW_054740 [Citrus unshiu][more]
KAF4368296.14.7e-7661.09hypothetical protein G4B88_008600 [Cannabis sativa][more]
Match NameE-valueIdentityDescription
O657593.1e-6293.18Histone H2AX OS=Cicer arietinum OX=3827 GN=HIS2A PE=2 SV=1[more]
Q9S9K78.9e-6293.18Probable histone H2AXb OS=Arabidopsis thaliana OX=3702 GN=At1g54690 PE=1 SV=1[more]
O048482.0e-6192.42Probable histone H2AXa OS=Arabidopsis thaliana OX=3702 GN=At1g08880 PE=1 SV=1[more]
P350632.0e-6192.48Histone H2AX OS=Picea abies OX=3329 PE=2 SV=1[more]
A2ZL692.1e-5890.08Probable histone H2AXb OS=Oryza sativa subsp. indica OX=39946 GN=OsI_037312 PE=3... [more]
Match NameE-valueIdentityDescription
A0A067JNE64.4e-8064.44Histone H2A OS=Jatropha curcas OX=180498 GN=JCGZ_22006 PE=3 SV=1[more]
A0A2H5NLL62.0e-7759.66Histone H2A OS=Citrus unshiu OX=55188 GN=CUMW_054740 PE=3 SV=1[more]
A0A7J6FC782.3e-7661.09Uncharacterized protein OS=Cannabis sativa OX=3483 GN=G4B88_008600 PE=3 SV=1[more]
A0A7J6EH342.0e-7254.74Uncharacterized protein OS=Cannabis sativa OX=3483 GN=F8388_007218 PE=3 SV=1[more]
A0A150G9R42.1e-6972.36Histone H2A OS=Gonium pectorale OX=33097 GN=GPECTOR_42g800 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G54690.16.3e-6393.18gamma histone variant H2AX [more]
AT1G08880.11.4e-6292.42Histone superfamily protein [more]
AT4G27230.11.3e-5287.90histone H2A 2 [more]
AT4G27230.21.3e-5287.90histone H2A 2 [more]
AT5G54640.18.6e-5286.29Histone superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002119Histone H2APRINTSPR00620HISTONEH2Acoord: 121..134
score: 89.46
coord: 135..149
score: 75.49
coord: 106..121
score: 84.58
coord: 77..99
score: 69.94
coord: 163..181
score: 70.48
IPR002119Histone H2ASMARTSM00414h2a4coord: 69..186
e-value: 6.5E-76
score: 268.2
coord: 8..68
e-value: 1.2E-4
score: 23.7
IPR002119Histone H2ACDDcd00074H2Acoord: 71..183
e-value: 6.17903E-66
score: 196.402
IPR009072Histone-foldGENE3D1.10.20.10Histone, subunit Acoord: 68..190
e-value: 1.1E-62
score: 211.6
IPR009072Histone-foldGENE3D1.10.20.10Histone, subunit Acoord: 7..67
e-value: 8.5E-25
score: 89.0
IPR009072Histone-foldSUPERFAMILY47113Histone-foldcoord: 5..67
IPR009072Histone-foldSUPERFAMILY47113Histone-foldcoord: 80..183
IPR007125Histone H2A/H2B/H3PFAMPF00125Histonecoord: 74..152
e-value: 3.1E-14
score: 53.5
IPR032454Histone H2A, C-terminal domainPFAMPF16211Histone_H2A_Ccoord: 155..188
e-value: 1.7E-18
score: 66.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..26
NoneNo IPR availablePANTHERPTHR23430:SF288HISTONE H2Acoord: 7..67
NoneNo IPR availablePANTHERPTHR23430HISTONE H2Acoord: 67..196
NoneNo IPR availablePANTHERPTHR23430HISTONE H2Acoord: 7..67
NoneNo IPR availablePANTHERPTHR23430:SF288HISTONE H2Acoord: 67..196
IPR032458Histone H2A conserved sitePROSITEPS00046HISTONE_H2Acoord: 85..91
IPR032458Histone H2A conserved sitePROSITEPS00046HISTONE_H2Acoord: 27..33

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg07217-RACarg07217-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0031507 heterochromatin assembly
cellular_component GO:0000786 nucleosome
cellular_component GO:0005634 nucleus
molecular_function GO:0003677 DNA binding
molecular_function GO:0046982 protein heterodimerization activity