Carg07213 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg07213
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Glutamic acid-rich protein
Location	Carg_Chr17: 8348067 .. 8348450 (+)
RNA-Seq Expression	Carg07213
Synteny	Carg07213

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTCTCAACTCCTTTCTTCTTCAACTTCCATCCTCACCAATTTGGCAACCACATTGAGGATTTTTCATCTCATTTGCTCTTCGAGGCGACGGGCGACTCCGAGGCCGACTCCTTGGTCGGCCACGGAAGCTCGACCGTCTCGGTGGAGTTCAACGACGCTGAATCTTGCACGGATGACACTCCTGCTATGCGTAACAAGGTCCAGACGTATGAAAACGATGACGATGACGATGACGATGACGAGAATGAGGAGGAGGTGGAAAGCAAGCCAATTGGATTCATGACAGAGTCAAGTGCTTCTATTGATTCAGCTGAGGAGTTCAAAATGTTGAATGAGGTGGAGAAAAACAGGTTGTTTTGGGAGACTTGTTTGGCTTCGTAA

mRNA sequence

ATGCTCTCAACTCCTTTCTTCTTCAACTTCCATCCTCACCAATTTGGCAACCACATTGAGGATTTTTCATCTCATTTGCTCTTCGAGGCGACGGGCGACTCCGAGGCCGACTCCTTGGTCGGCCACGGAAGCTCGACCGTCTCGGTGGAGTTCAACGACGCTGAATCTTGCACGGATGACACTCCTGCTATGCGTAACAAGGTCCAGACGTATGAAAACGATGACGATGACGATGACGATGACGAGAATGAGGAGGAGGTGGAAAGCAAGCCAATTGGATTCATGACAGAGTCAAGTGCTTCTATTGATTCAGCTGAGGAGTTCAAAATGTTGAATGAGGTGGAGAAAAACAGGTTGTTTTGGGAGACTTGTTTGGCTTCGTAA

Coding sequence (CDS)

ATGCTCTCAACTCCTTTCTTCTTCAACTTCCATCCTCACCAATTTGGCAACCACATTGAGGATTTTTCATCTCATTTGCTCTTCGAGGCGACGGGCGACTCCGAGGCCGACTCCTTGGTCGGCCACGGAAGCTCGACCGTCTCGGTGGAGTTCAACGACGCTGAATCTTGCACGGATGACACTCCTGCTATGCGTAACAAGGTCCAGACGTATGAAAACGATGACGATGACGATGACGATGACGAGAATGAGGAGGAGGTGGAAAGCAAGCCAATTGGATTCATGACAGAGTCAAGTGCTTCTATTGATTCAGCTGAGGAGTTCAAAATGTTGAATGAGGTGGAGAAAAACAGGTTGTTTTGGGAGACTTGTTTGGCTTCGTAA

Protein sequence

MLSTPFFFNFHPHQFGNHIEDFSSHLLFEATGDSEADSLVGHGSSTVSVEFNDAESCTDDTPAMRNKVQTYENDDDDDDDDENEEEVESKPIGFMTESSASIDSAEEFKMLNEVEKNRLFWETCLAS

Homology

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BLAST of Carg07213 vs. NCBI nr
Match: KAG6575870.1 (hypothetical protein SDJN03_26509, partial [Cucurbita argyrosperma subsp. sororia] >KAG7014404.1 hypothetical protein SDJN02_24581, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 250.4 bits (638), Expect = 8.5e-63
Identity = 127/127 (100.00%), Postives = 127/127 (100.00%), Query Frame = 0


Query: 1   MLSTPFFFNFHPHQFGNHIEDFSSHLLFEATGDSEADSLVGHGSSTVSVEFNDAESCTDD 60
           MLSTPFFFNFHPHQFGNHIEDFSSHLLFEATGDSEADSLVGHGSSTVSVEFNDAESCTDD
Sbjct: 1   MLSTPFFFNFHPHQFGNHIEDFSSHLLFEATGDSEADSLVGHGSSTVSVEFNDAESCTDD 60

Query: 61  TPAMRNKVQTYENDDDDDDDDENEEEVESKPIGFMTESSASIDSAEEFKMLNEVEKNRLF 120
           TPAMRNKVQTYENDDDDDDDDENEEEVESKPIGFMTESSASIDSAEEFKMLNEVEKNRLF
Sbjct: 61  TPAMRNKVQTYENDDDDDDDDENEEEVESKPIGFMTESSASIDSAEEFKMLNEVEKNRLF 120

Query: 121 WETCLAS 128
           WETCLAS
Sbjct: 121 WETCLAS 127

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BLAST of Carg07213 vs. NCBI nr
Match: XP_022991734.1 (uncharacterized protein LOC111488264 [Cucurbita maxima])

HSP 1 Score: 235.3 bits (599), Expect = 2.8e-58
Identity = 121/128 (94.53%), Postives = 125/128 (97.66%), Query Frame = 0


Query: 1   MLSTPFFFNFHPHQFGNHIEDFSSHLLFEATGDSEADSLVGHGSSTVSVEFNDAESCTDD 60
           MLSTPFFFNFHPHQFGNHIEDFSSHLLFEATGDSEADSLVGHGSSTVSVEFNDAESCTDD
Sbjct: 1   MLSTPFFFNFHPHQFGNHIEDFSSHLLFEATGDSEADSLVGHGSSTVSVEFNDAESCTDD 60

Query: 61  TPAMRNKVQTYENDDDDDDDD-ENEEEVESKPIGFMTESSASIDSAEEFKMLNEVEKNRL 120
           TPAM N+ +TYENDDDDDDDD EN+EEVESKPIGFMTESSASIDSAEEFKMLNEV+KNRL
Sbjct: 61  TPAMCNEFKTYENDDDDDDDDEENDEEVESKPIGFMTESSASIDSAEEFKMLNEVDKNRL 120

Query: 121 FWETCLAS 128
           FWETCLAS
Sbjct: 121 FWETCLAS 128

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BLAST of Carg07213 vs. NCBI nr
Match: XP_022953969.1 (uncharacterized protein LOC111456367 [Cucurbita moschata])

HSP 1 Score: 233.8 bits (595), Expect = 8.2e-58
Identity = 121/127 (95.28%), Postives = 123/127 (96.85%), Query Frame = 0


Query: 1   MLSTPFFFNFHPHQFGNHIEDFSSHLLFEATGDSEADSLVGHGSSTVSVEFNDAESCTDD 60
           MLSTPFFFNFHPHQ+G HIEDFSS LLFEATGDSEADSLVGHGSSTVSVEFNDAESCTD+
Sbjct: 1   MLSTPFFFNFHPHQYGTHIEDFSSQLLFEATGDSEADSLVGHGSSTVSVEFNDAESCTDE 60

Query: 61  TPAMRNKVQTYENDDDDDDDDENEEEVESKPIGFMTESSASIDSAEEFKMLNEVEKNRLF 120
           TPAMRNKVQTYE  DDDDDDDENEEEVESKPIGFMTESSASIDSAEEFKMLNEVEKNRLF
Sbjct: 61  TPAMRNKVQTYE--DDDDDDDENEEEVESKPIGFMTESSASIDSAEEFKMLNEVEKNRLF 120

Query: 121 WETCLAS 128
           WETCLAS
Sbjct: 121 WETCLAS 125

[Close]

BLAST of Carg07213 vs. NCBI nr
Match: XP_023522205.1 (uncharacterized protein LOC111786091 [Cucurbita pepo subsp. pepo] >XP_023548487.1 uncharacterized protein LOC111807136 [Cucurbita pepo subsp. pepo] >XP_023549405.1 uncharacterized protein LOC111807769 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 231.9 bits (590), Expect = 3.1e-57
Identity = 119/127 (93.70%), Postives = 121/127 (95.28%), Query Frame = 0


Query: 1   MLSTPFFFNFHPHQFGNHIEDFSSHLLFEATGDSEADSLVGHGSSTVSVEFNDAESCTDD 60
           MLSTPFFFNFHPHQFGNHIEDFSSHLLFEATGDSEADSLVGHGSSTVSVEFNDAESCTDD
Sbjct: 1   MLSTPFFFNFHPHQFGNHIEDFSSHLLFEATGDSEADSLVGHGSSTVSVEFNDAESCTDD 60

Query: 61  TPAMRNKVQTYENDDDDDDDDENEEEVESKPIGFMTESSASIDSAEEFKMLNEVEKNRLF 120
           TPAMRN+VQ YEN    DDDDEN+EEVESKPIGFMTESS SIDSAEEFKMLNEVEKNRLF
Sbjct: 61  TPAMRNEVQMYEN----DDDDENDEEVESKPIGFMTESSGSIDSAEEFKMLNEVEKNRLF 120

Query: 121 WETCLAS 128
           WETCLAS
Sbjct: 121 WETCLAS 123

[Close]

BLAST of Carg07213 vs. NCBI nr
Match: XP_038896353.1 (uncharacterized protein LOC120084618 [Benincasa hispida])

HSP 1 Score: 154.5 bits (389), Expect = 6.3e-34
Identity = 87/135 (64.44%), Postives = 103/135 (76.30%), Query Frame = 0


Query: 1   MLSTPFFFNFHPHQF----GNHIEDFSSHLLFEATGDSEADSLVGHGSSTVSVEFNDAES 60
           MLSTP FFNF+PH F    GNHIED SS+ L EATGDSE DS V  G S  S EFNDAES
Sbjct: 1   MLSTPLFFNFYPHPFAHKQGNHIEDISSYFLLEATGDSEIDSSVDLGGSVASTEFNDAES 60

Query: 61  CTDDTPAM-RNKVQTYENDDDDDD---DDENEEEVESKPIGFMTESSASIDSAEEFKMLN 120
           CTDDTP +  N+   YE +D+D++   +++ +E VESK IGF T+S+ASIDS +EFKMLN
Sbjct: 61  CTDDTPTICTNEFGKYEYEDEDEEEMVENDYDEVVESKAIGFTTKSNASIDSTKEFKMLN 120

Query: 121 EVEKNRLFWETCLAS 128
           EV+KNRLFWETCLAS
Sbjct: 121 EVDKNRLFWETCLAS 135

[Close]

BLAST of Carg07213 vs. ExPASy TrEMBL
Match: A0A6J1JVN0 (uncharacterized protein LOC111488264 OS=Cucurbita maxima OX=3661 GN=LOC111488264 PE=4 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 1.4e-58
Identity = 121/128 (94.53%), Postives = 125/128 (97.66%), Query Frame = 0


Query: 1   MLSTPFFFNFHPHQFGNHIEDFSSHLLFEATGDSEADSLVGHGSSTVSVEFNDAESCTDD 60
           MLSTPFFFNFHPHQFGNHIEDFSSHLLFEATGDSEADSLVGHGSSTVSVEFNDAESCTDD
Sbjct: 1   MLSTPFFFNFHPHQFGNHIEDFSSHLLFEATGDSEADSLVGHGSSTVSVEFNDAESCTDD 60

Query: 61  TPAMRNKVQTYENDDDDDDDD-ENEEEVESKPIGFMTESSASIDSAEEFKMLNEVEKNRL 120
           TPAM N+ +TYENDDDDDDDD EN+EEVESKPIGFMTESSASIDSAEEFKMLNEV+KNRL
Sbjct: 61  TPAMCNEFKTYENDDDDDDDDEENDEEVESKPIGFMTESSASIDSAEEFKMLNEVDKNRL 120

Query: 121 FWETCLAS 128
           FWETCLAS
Sbjct: 121 FWETCLAS 128

[Close]

BLAST of Carg07213 vs. ExPASy TrEMBL
Match: A0A6J1GRH1 (uncharacterized protein LOC111456367 OS=Cucurbita moschata OX=3662 GN=LOC111456367 PE=4 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 4.0e-58
Identity = 121/127 (95.28%), Postives = 123/127 (96.85%), Query Frame = 0


Query: 1   MLSTPFFFNFHPHQFGNHIEDFSSHLLFEATGDSEADSLVGHGSSTVSVEFNDAESCTDD 60
           MLSTPFFFNFHPHQ+G HIEDFSS LLFEATGDSEADSLVGHGSSTVSVEFNDAESCTD+
Sbjct: 1   MLSTPFFFNFHPHQYGTHIEDFSSQLLFEATGDSEADSLVGHGSSTVSVEFNDAESCTDE 60

Query: 61  TPAMRNKVQTYENDDDDDDDDENEEEVESKPIGFMTESSASIDSAEEFKMLNEVEKNRLF 120
           TPAMRNKVQTYE  DDDDDDDENEEEVESKPIGFMTESSASIDSAEEFKMLNEVEKNRLF
Sbjct: 61  TPAMRNKVQTYE--DDDDDDDENEEEVESKPIGFMTESSASIDSAEEFKMLNEVEKNRLF 120

Query: 121 WETCLAS 128
           WETCLAS
Sbjct: 121 WETCLAS 125

[Close]

BLAST of Carg07213 vs. ExPASy TrEMBL
Match: A0A5A7UVG0 (Glutamic acid-rich protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold497G00160 PE=4 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 6.4e-32
Identity = 91/147 (61.90%), Postives = 103/147 (70.07%), Query Frame = 0


Query: 1   MLST-PFFFNFHPHQF----GNHIEDFSSHLLFEATGDSEADSLVG--HGSSTVSVEFND 60
           MLST P FFNF+PH F    GNHIED SS+ LFEATGDSEADS V     SS  + EFND
Sbjct: 1   MLSTSPLFFNFYPHHFVHNQGNHIEDISSYFLFEATGDSEADSSVDLESSSSASTTEFND 60

Query: 61  AESCTDDTPAM------------RNKVQTYENDDDDDDDDENEEEVESKPIGFMTESSAS 120
           AESCTDDTP M             +  +  ENDDDD D++E EE VESK IGF  +S+AS
Sbjct: 61  AESCTDDTPTMLCNNENEDDDDDGDGEEMVENDDDDHDNEEEEEVVESKAIGFSIKSNAS 120

Query: 121 IDSA-EEFKMLNEVEKNRLFWETCLAS 128
           IDS  ++FKMLNEV+KNRLFWETCLAS
Sbjct: 121 IDSTKDDFKMLNEVDKNRLFWETCLAS 147

[Close]

BLAST of Carg07213 vs. ExPASy TrEMBL
Match: A0A1S3BRI2 (glutamic acid-rich protein OS=Cucumis melo OX=3656 GN=LOC103492721 PE=4 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 2.4e-31
Identity = 92/150 (61.33%), Postives = 104/150 (69.33%), Query Frame = 0


Query: 1   MLST-PFFFNFHPHQF----GNHIEDFSSHLLFEATGDSEADSLVG--HGSSTVSVEFND 60
           MLST P FFNF+PH F    GNHIED SS+ LFEATGDSEADS V     SS  + EFND
Sbjct: 1   MLSTSPLFFNFYPHHFVHNQGNHIEDISSYFLFEATGDSEADSSVDLESSSSASTTEFND 60

Query: 61  AESCTDDTPAM-------------RNKVQTYENDDDDDDDDENEEE--VESKPIGFMTES 120
           AESCTDDTP M              +  +  ENDDDD DD++ EEE  VESK IGF  +S
Sbjct: 61  AESCTDDTPTMLCNNENEDDDDDDGDGEEMVENDDDDHDDEDEEEEEVVESKAIGFSIKS 120

Query: 121 SASIDSA-EEFKMLNEVEKNRLFWETCLAS 128
           +ASIDS  ++FKMLNEV+KNRLFWETCLAS
Sbjct: 121 NASIDSTKDDFKMLNEVDKNRLFWETCLAS 150

[Close]

BLAST of Carg07213 vs. ExPASy TrEMBL
Match: A0A0A0K519 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G390190 PE=4 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 4.1e-31
Identity = 89/142 (62.68%), Postives = 100/142 (70.42%), Query Frame = 0


Query: 1   MLSTPFFFNFHPHQF----GNHIEDFSSHLLFEATGDSEADSLVGHGSSTVSVEFNDAES 60
           MLS+P FFNF+PH F    GNHIED SS+ LFEATGDSE D      SS VS EFNDAES
Sbjct: 1   MLSSPLFFNFYPHHFVHNQGNHIEDISSYFLFEATGDSEVDL---QSSSPVSTEFNDAES 60

Query: 61  CTDDTPAMR---------NKVQTYENDDDDDDDDENEEE-VESKPIGFMTESSASIDSA- 120
           CTDDT  +           +    ENDDDD D++E EEE VESK IGF  +S+ASIDS  
Sbjct: 61  CTDDTDRIMLCNNEDDEDEEEMGVENDDDDGDEEEEEEEVVESKAIGFSIKSNASIDSTK 120

Query: 121 EEFKMLNEVEKNRLFWETCLAS 128
           +EFKMLNEV+KNRLFWETCLAS
Sbjct: 121 DEFKMLNEVDKNRLFWETCLAS 139

[Close]

BLAST of Carg07213 vs. TAIR 10
Match: AT2G47950.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; EXPRESSED IN: root, flower; EXPRESSED DURING: petal differentiation and expansion stage; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G62990.1); Has 22 Blast hits to 22 proteins in 5 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 22; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 42.7 bits (99), Expect = 2.5e-04
Identity = 41/131 (31.30%), Postives = 64/131 (48.85%), Query Frame = 0


Query: 17  NHIEDFSSHLLFEATGDSEADSLVGHGS---------STVSVEFNDAESCTDDTPAMRNK 76
           N++ D S  LL EA+ DSEA    GH S           +  E + A SC     + R  
Sbjct: 5   NNVFDVSPFLLLEASADSEA----GHDSVDDDKCVKDYDLGHESSSASSCETSCVSQRTS 64

Query: 77  VQTYENDDD----------DDDDDENEEEVESK-PIGFMTESSASIDSAEEFKMLNEVEK 128
           +  ++  DD          D++D++ E EV S    G     + ++DSA    +++E+++
Sbjct: 65  LLGFDLQDDTVNHEERDAGDEEDEDGEGEVNSYIRCGRSQRENLAVDSA---AVVSEMDQ 124

The following BLAST results are available for this feature:

BLAST of Carg07213 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6575870.1	8.5e-63	100.00	hypothetical protein SDJN03_26509, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022991734.1	2.8e-58	94.53	uncharacterized protein LOC111488264 [Cucurbita maxima]	[more]
XP_022953969.1	8.2e-58	95.28	uncharacterized protein LOC111456367 [Cucurbita moschata]	[more]
XP_023522205.1	3.1e-57	93.70	uncharacterized protein LOC111786091 [Cucurbita pepo subsp. pepo] >XP_023548487....	[more]
XP_038896353.1	6.3e-34	64.44	uncharacterized protein LOC120084618 [Benincasa hispida]	[more]

BLAST of Carg07213 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Carg07213 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JVN0	1.4e-58	94.53	uncharacterized protein LOC111488264 OS=Cucurbita maxima OX=3661 GN=LOC111488264...	[more]
A0A6J1GRH1	4.0e-58	95.28	uncharacterized protein LOC111456367 OS=Cucurbita moschata OX=3662 GN=LOC1114563...	[more]
A0A5A7UVG0	6.4e-32	61.90	Glutamic acid-rich protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff...	[more]
A0A1S3BRI2	2.4e-31	61.33	glutamic acid-rich protein OS=Cucumis melo OX=3656 GN=LOC103492721 PE=4 SV=1	[more]
A0A0A0K519	4.1e-31	62.68	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G390190 PE=4 SV=1	[more]

BLAST of Carg07213 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G47950.1	2.5e-04	31.30	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 51..98
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 73..87
None	No IPR available	PANTHER	PTHR35726	GLUTAMIC ACID-RICH PROTEIN-LIKE	coord: 17..127
None	No IPR available	PANTHER	PTHR35726:SF4	GLUTAMIC ACID-RICH PROTEIN-LIKE	coord: 17..127

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg07213-RA	Carg07213-RA	mRNA