Carg07156 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg07156
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionPectinesterase
LocationCarg_Chr17: 8060470 .. 8063435 (+)
RNA-Seq ExpressionCarg07156
SyntenyCarg07156
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTATTATTTTAAAAAGTATGAAATATTATAAATCTACTCTTCATCTTCCTTCTTGACAGCCACATAAATCTCCCCTTCATCTTCTTCCCTCCTCTCTGTTTCAGACATGTACGGCCTGAGAAGCAGAGCTCAAAGCTCTTCAAATCCCACCGCCTCCATTTCCGCCATTTTCACCTTCTTTTCCGTTCTTTTCCTTTGCTCCTTCAAACCCATTTCTTCAAATCCCCAAACCCACCAAACCGCTCGATCCCTTTGCAATGGCACTCTGTTTCCAAATCTCTGTTTCTCAAAGCTCTCCTCTTTTCCCCAACTCGCCTCCCTCTCGCCGGAAAAGCTCGCCGGCTCCGCCCTCAACTTCACCAACGACGAGGTTCACGGTGCCTACGCCAATTGCTCCAAGCTCAAGACTCTCTTCTACGGAGGCCTGAATCCCACCGACCGCCGCGCCCTCGATGACTGCCTTGAGCTTCTCGATGACAGCATTGCCGAACTCCAAACTTCGATTTACGATCTTTCCCCGTCGCAGTCCGCCGCCCGCCACTCTCACGATCTGTTGACGTTGGTCAGCGCCGCCATGACTAACCACCGGACTTGTCTTGATGGGTTTTATAATAGTTCCGGGAATGTCAGGAAACGGGTGGAGCTGTATTTGGGGAAAATCGAACAGCATTTGAGTATTGATTTGGCTATGCTGAAGAAAATCCCTGGAGTTAAACGGGCTGCCACGGCGGAAGAGGTGTTGCCGGAGTATGGGGCGGTGAGAGGTGGGTTTCCGATGTGGGTTTCGGTGAAGGATCGGAGATTGTTGCAGGCGGCGGTGAATGAAACGAAGTTCAATTTGGTGGTGGCGAAGGATGGCTCCGGGAATTTCACCACCGTCGGCGAGGCTGTCGCCGCCGCTCCAAATTCGAGCACGACCAGGTTTGTAATATACATAAAAGCAGGGGCCTATTTTGAGAATGTGGAAATAGAACGGAAAAAGACAAACTTGATGTTCGTCGGCGACGGGATCGGCAAGACACTGATAAAGGCCGACCGGAATGTCGTCGACGGCTGGACTACCTTCCGATCAGCCACCGTCGGTCAGTTCTCTGTTTCACTCTTTTTTTTAATAAATAAATAATTAATTACTATCATTAAAATGCTGAAACCACCTGACGACCATCAGATCTTGCCTCTGAACTAATTTATTTATTTATTAATCATAATTTTGGATCTGGTCTAATATTTTAAAATTAGAAAACGTCTTAATTTAATGTGTTTTTTTTTTTTTAATTTTAGAAAATAAATAGGTATAGAATTGAAACTTAAAAAAGAAAATCAAAATTTAAAATAGAAAAGGGAAATTAGTAGATAATAGTTGAATATAAAATAGACTAAACTAATAAAAAAAAACATTTTTAAAATTTTAAAAGTTTAATAATACAAAATTAAAAAAAAAATTAATATTTTATTTAAAAAATATCTTTTAATTTTTATAAATTCCCATAAAGTTTCAAAAGTTTCATTATATAATTTTTTTTTTTTTAATGTCATTAAATATTTTAAATATTTTTAAAAATAATACGGTTAATATCCATTTAAAAATACTAATAAACTTCTAAAAGTAACACGAGATAATAGATTGTTTTCATTTATATGTTGACGATAATTTTTTTTGAATTTTTTTATTTATAAAAATTAAGGAATTAATGTTACAGGTGTTTTCAAATAAAAATTTAAATTAAAAGGTATTAATAAAAAAAAATATTATTGCAAAATTGATAATTTTTAAAACATTTAAAATTATTGTTGAATTTTTTGAAAATTTTGGTATGTTTTTTTATTAATTATTTTGAAAGTGGCAGAAGGGTAGGGGAAGGCGAGTACAGTGGGCCCAGTGGGCCCAGTGTGGCCCAAAAGGCGCTAATATGGGCGAACCGCATGTGGGTTGGATAAGACTGCCAATCTCCGGAAAAAAGGGCCGTTGCCTTTTTCAATTAATTTTTCAATTTATTCTATAAATTTATAAATGTTTTTTAAATCTACTTTTTTTATTTGGTCTTATAATTTTCTGTTGACAGTTTTAGTTTTAATATTTGGTATTTGATGTTAAATTTTTTTTAATTTTTTCTTTCGAGAATAATAACATATGTTAGTTACTATGTTCAGCGGCTGTCGGCAATGGGTTCATAGCGAAAGGCATAACATTCGAAAACTTTGCTGGTCCGAGCAAGCACCAAGCCGTAGCCTTCCGAAGCAACTCCGACTTCTCAGCATTTTACCAATGCAGCTTCATCGGCTATCAAGACACTCTCTACGTCCATTCTCTCCGTCAATTCTACCGCGAATGCGACGTCTATGGCACCATCGACTTCATCTTCGGTAACGCAGCAGTCGTCTTCCAAAACTGCAATCTATATGCTCGAAAACCCAACTCAAACCAACGCAACATATTCACAGCTCAAGGTCGTGAGGATCCTAACCAAAATACCGGAATCTCAATCCTCAACTGCAAGGTTGAAGCCGCTTCTGATTTGCTCCCAGTTCTTCCCTCTTTTAGAACTTACTTGGGTCGACCCTGGAAGCTCTACTCGAGGACGGTTTTTCTCAAGTCATTTATTGGGCAGTTGGTTGAGCCGGTCGGATGGCTTGAGTGGAGTGGGACATTCGCATTAGATACTTTGTATTATGGGGAGTATTTGAATCGTGGACCTGGTTCTAATACAACTGGACGAGTTACTTGGCCAGGTTATCGGGTCATTACTAACTCAACAGAAGCGAGTCAGTTTACAGTGGAGGCGTTTATACAGGGTAGTGAGTGGCTGAACTCGACCGAGATTCCTTTCTTCTCTGGTTTGGCTTGACCGTTATCCCTCGAAGGTTCAATGTTTCGCTGCTTTTTGTTTTTGTAGGGACTAAAATGGTTTGACGTTGTTCGAGAAAGTTAAAAGTTGCTTAATCTAAGATTATATATCTAATTATC

mRNA sequence

ATTATTATTTTAAAAAGTATGAAATATTATAAATCTACTCTTCATCTTCCTTCTTGACAGCCACATAAATCTCCCCTTCATCTTCTTCCCTCCTCTCTGTTTCAGACATGTACGGCCTGAGAAGCAGAGCTCAAAGCTCTTCAAATCCCACCGCCTCCATTTCCGCCATTTTCACCTTCTTTTCCGTTCTTTTCCTTTGCTCCTTCAAACCCATTTCTTCAAATCCCCAAACCCACCAAACCGCTCGATCCCTTTGCAATGGCACTCTGTTTCCAAATCTCTGTTTCTCAAAGCTCTCCTCTTTTCCCCAACTCGCCTCCCTCTCGCCGGAAAAGCTCGCCGGCTCCGCCCTCAACTTCACCAACGACGAGGTTCACGGTGCCTACGCCAATTGCTCCAAGCTCAAGACTCTCTTCTACGGAGGCCTGAATCCCACCGACCGCCGCGCCCTCGATGACTGCCTTGAGCTTCTCGATGACAGCATTGCCGAACTCCAAACTTCGATTTACGATCTTTCCCCGTCGCAGTCCGCCGCCCGCCACTCTCACGATCTGTTGACGTTGGTCAGCGCCGCCATGACTAACCACCGGACTTGTCTTGATGGGTTTTATAATAGTTCCGGGAATGTCAGGAAACGGGTGGAGCTGTATTTGGGGAAAATCGAACAGCATTTGAGTATTGATTTGGCTATGCTGAAGAAAATCCCTGGAGTTAAACGGGCTGCCACGGCGGAAGAGGTGTTGCCGGAGTATGGGGCGGTGAGAGGTGGGTTTCCGATGTGGGTTTCGGTGAAGGATCGGAGATTGTTGCAGGCGGCGGTGAATGAAACGAAGTTCAATTTGGTGGTGGCGAAGGATGGCTCCGGGAATTTCACCACCGTCGGCGAGGCTGTCGCCGCCGCTCCAAATTCGAGCACGACCAGGTTTGTAATATACATAAAAGCAGGGGCCTATTTTGAGAATGTGGAAATAGAACGGAAAAAGACAAACTTGATGTTCGTCGGCGACGGGATCGGCAAGACACTGATAAAGGCCGACCGGAATGTCGTCGACGGCTGGACTACCTTCCGATCAGCCACCGTCGCGGCTGTCGGCAATGGGTTCATAGCGAAAGGCATAACATTCGAAAACTTTGCTGGTCCGAGCAAGCACCAAGCCGTAGCCTTCCGAAGCAACTCCGACTTCTCAGCATTTTACCAATGCAGCTTCATCGGCTATCAAGACACTCTCTACGTCCATTCTCTCCGTCAATTCTACCGCGAATGCGACGTCTATGGCACCATCGACTTCATCTTCGGTAACGCAGCAGTCGTCTTCCAAAACTGCAATCTATATGCTCGAAAACCCAACTCAAACCAACGCAACATATTCACAGCTCAAGGTCGTGAGGATCCTAACCAAAATACCGGAATCTCAATCCTCAACTGCAAGGTTGAAGCCGCTTCTGATTTGCTCCCAGTTCTTCCCTCTTTTAGAACTTACTTGGGTCGACCCTGGAAGCTCTACTCGAGGACGGTTTTTCTCAAGTCATTTATTGGGCAGTTGGTTGAGCCGGTCGGATGGCTTGAGTGGAGTGGGACATTCGCATTAGATACTTTGTATTATGGGGAGTATTTGAATCGTGGACCTGGTTCTAATACAACTGGACGAGTTACTTGGCCAGGTTATCGGGTCATTACTAACTCAACAGAAGCGAGTCAGTTTACAGTGGAGGCGTTTATACAGGGTAGTGAGTGGCTGAACTCGACCGAGATTCCTTTCTTCTCTGGTTTGGCTTGACCGTTATCCCTCGAAGGTTCAATGTTTCGCTGCTTTTTGTTTTTGTAGGGACTAAAATGGTTTGACGTTGTTCGAGAAAGTTAAAAGTTGCTTAATCTAAGATTATATATCTAATTATC

Coding sequence (CDS)

ATGTACGGCCTGAGAAGCAGAGCTCAAAGCTCTTCAAATCCCACCGCCTCCATTTCCGCCATTTTCACCTTCTTTTCCGTTCTTTTCCTTTGCTCCTTCAAACCCATTTCTTCAAATCCCCAAACCCACCAAACCGCTCGATCCCTTTGCAATGGCACTCTGTTTCCAAATCTCTGTTTCTCAAAGCTCTCCTCTTTTCCCCAACTCGCCTCCCTCTCGCCGGAAAAGCTCGCCGGCTCCGCCCTCAACTTCACCAACGACGAGGTTCACGGTGCCTACGCCAATTGCTCCAAGCTCAAGACTCTCTTCTACGGAGGCCTGAATCCCACCGACCGCCGCGCCCTCGATGACTGCCTTGAGCTTCTCGATGACAGCATTGCCGAACTCCAAACTTCGATTTACGATCTTTCCCCGTCGCAGTCCGCCGCCCGCCACTCTCACGATCTGTTGACGTTGGTCAGCGCCGCCATGACTAACCACCGGACTTGTCTTGATGGGTTTTATAATAGTTCCGGGAATGTCAGGAAACGGGTGGAGCTGTATTTGGGGAAAATCGAACAGCATTTGAGTATTGATTTGGCTATGCTGAAGAAAATCCCTGGAGTTAAACGGGCTGCCACGGCGGAAGAGGTGTTGCCGGAGTATGGGGCGGTGAGAGGTGGGTTTCCGATGTGGGTTTCGGTGAAGGATCGGAGATTGTTGCAGGCGGCGGTGAATGAAACGAAGTTCAATTTGGTGGTGGCGAAGGATGGCTCCGGGAATTTCACCACCGTCGGCGAGGCTGTCGCCGCCGCTCCAAATTCGAGCACGACCAGGTTTGTAATATACATAAAAGCAGGGGCCTATTTTGAGAATGTGGAAATAGAACGGAAAAAGACAAACTTGATGTTCGTCGGCGACGGGATCGGCAAGACACTGATAAAGGCCGACCGGAATGTCGTCGACGGCTGGACTACCTTCCGATCAGCCACCGTCGCGGCTGTCGGCAATGGGTTCATAGCGAAAGGCATAACATTCGAAAACTTTGCTGGTCCGAGCAAGCACCAAGCCGTAGCCTTCCGAAGCAACTCCGACTTCTCAGCATTTTACCAATGCAGCTTCATCGGCTATCAAGACACTCTCTACGTCCATTCTCTCCGTCAATTCTACCGCGAATGCGACGTCTATGGCACCATCGACTTCATCTTCGGTAACGCAGCAGTCGTCTTCCAAAACTGCAATCTATATGCTCGAAAACCCAACTCAAACCAACGCAACATATTCACAGCTCAAGGTCGTGAGGATCCTAACCAAAATACCGGAATCTCAATCCTCAACTGCAAGGTTGAAGCCGCTTCTGATTTGCTCCCAGTTCTTCCCTCTTTTAGAACTTACTTGGGTCGACCCTGGAAGCTCTACTCGAGGACGGTTTTTCTCAAGTCATTTATTGGGCAGTTGGTTGAGCCGGTCGGATGGCTTGAGTGGAGTGGGACATTCGCATTAGATACTTTGTATTATGGGGAGTATTTGAATCGTGGACCTGGTTCTAATACAACTGGACGAGTTACTTGGCCAGGTTATCGGGTCATTACTAACTCAACAGAAGCGAGTCAGTTTACAGTGGAGGCGTTTATACAGGGTAGTGAGTGGCTGAACTCGACCGAGATTCCTTTCTTCTCTGGTTTGGCTTGA

Protein sequence

MYGLRSRAQSSSNPTASISAIFTFFSVLFLCSFKPISSNPQTHQTARSLCNGTLFPNLCFSKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLELLDDSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGNVRKRVELYLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTLIKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLVEPVGWLEWSGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQGSEWLNSTEIPFFSGLA
Homology
BLAST of Carg07156 vs. NCBI nr
Match: KAG7014347.1 (putative pectinesterase/pectinesterase inhibitor 40 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1118.2 bits (2891), Expect = 0.0e+00
Identity = 556/556 (100.00%), Postives = 556/556 (100.00%), Query Frame = 0

Query: 1   MYGLRSRAQSSSNPTASISAIFTFFSVLFLCSFKPISSNPQTHQTARSLCNGTLFPNLCF 60
           MYGLRSRAQSSSNPTASISAIFTFFSVLFLCSFKPISSNPQTHQTARSLCNGTLFPNLCF
Sbjct: 1   MYGLRSRAQSSSNPTASISAIFTFFSVLFLCSFKPISSNPQTHQTARSLCNGTLFPNLCF 60

Query: 61  SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE 120
           SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE
Sbjct: 61  SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE 120

Query: 121 LLDDSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGNVRKRVEL 180
           LLDDSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGNVRKRVEL
Sbjct: 121 LLDDSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGNVRKRVEL 180

Query: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE 240
           YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE
Sbjct: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE 240

Query: 241 TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGD 300
           TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGD
Sbjct: 241 TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGD 300

Query: 301 GIGKTLIKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFS 360
           GIGKTLIKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFS
Sbjct: 301 GIGKTLIKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFS 360

Query: 361 AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI 420
           AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI
Sbjct: 361 AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI 420

Query: 421 FTAQGREDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLV 480
           FTAQGREDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLV
Sbjct: 421 FTAQGREDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLV 480

Query: 481 EPVGWLEWSGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQG 540
           EPVGWLEWSGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQG
Sbjct: 481 EPVGWLEWSGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQG 540

Query: 541 SEWLNSTEIPFFSGLA 557
           SEWLNSTEIPFFSGLA
Sbjct: 541 SEWLNSTEIPFFSGLA 556

BLAST of Carg07156 vs. NCBI nr
Match: KAG6575805.1 (putative pectinesterase/pectinesterase inhibitor 40, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1110.1 bits (2870), Expect = 0.0e+00
Identity = 551/556 (99.10%), Postives = 554/556 (99.64%), Query Frame = 0

Query: 1   MYGLRSRAQSSSNPTASISAIFTFFSVLFLCSFKPISSNPQTHQTARSLCNGTLFPNLCF 60
           MYGLRSRAQSSSNPTASISAIFTFFSV+FLCSFKPISSNPQTHQTARSLCNGTLFPNLCF
Sbjct: 1   MYGLRSRAQSSSNPTASISAIFTFFSVIFLCSFKPISSNPQTHQTARSLCNGTLFPNLCF 60

Query: 61  SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE 120
           SKLSSFPQLASLSPEKLAGSALNFTN+EVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE
Sbjct: 61  SKLSSFPQLASLSPEKLAGSALNFTNNEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE 120

Query: 121 LLDDSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGNVRKRVEL 180
           LLD SIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSG VRKRVEL
Sbjct: 121 LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL 180

Query: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE 240
           YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE
Sbjct: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE 240

Query: 241 TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGD 300
           TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGD
Sbjct: 241 TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGD 300

Query: 301 GIGKTLIKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFS 360
           GIGKTLIKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFS
Sbjct: 301 GIGKTLIKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFS 360

Query: 361 AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI 420
           AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI
Sbjct: 361 AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI 420

Query: 421 FTAQGREDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLV 480
           FTAQGREDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLV
Sbjct: 421 FTAQGREDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLV 480

Query: 481 EPVGWLEWSGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQG 540
           EPVGWLEWSGTFALDTLYYGEYLNRGPGSNTTGR+TWPGYRVITNSTEASQFTVEAFIQG
Sbjct: 481 EPVGWLEWSGTFALDTLYYGEYLNRGPGSNTTGRITWPGYRVITNSTEASQFTVEAFIQG 540

Query: 541 SEWLNSTEIPFFSGLA 557
           SEWLNSTEIPFFSGLA
Sbjct: 541 SEWLNSTEIPFFSGLA 556

BLAST of Carg07156 vs. NCBI nr
Match: XP_022953706.1 (pectinesterase-like [Cucurbita moschata])

HSP 1 Score: 1107.8 bits (2864), Expect = 0.0e+00
Identity = 550/556 (98.92%), Postives = 552/556 (99.28%), Query Frame = 0

Query: 1   MYGLRSRAQSSSNPTASISAIFTFFSVLFLCSFKPISSNPQTHQTARSLCNGTLFPNLCF 60
           MYGLRSRAQSSSNPTASISAIFTFFSVLFLCSFKPISSNPQTHQTARSLCNGTLFPNLCF
Sbjct: 1   MYGLRSRAQSSSNPTASISAIFTFFSVLFLCSFKPISSNPQTHQTARSLCNGTLFPNLCF 60

Query: 61  SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE 120
           SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE
Sbjct: 61  SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE 120

Query: 121 LLDDSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGNVRKRVEL 180
           LLD SIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSG VRKRVEL
Sbjct: 121 LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL 180

Query: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE 240
           YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQA+VNE
Sbjct: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQASVNE 240

Query: 241 TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGD 300
           TKFNLVVAKDGSGNFTTVG AVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGD
Sbjct: 241 TKFNLVVAKDGSGNFTTVGAAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGD 300

Query: 301 GIGKTLIKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFS 360
           GIGKTLIKADRNVVDGWTTFRSATV AVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFS
Sbjct: 301 GIGKTLIKADRNVVDGWTTFRSATVVAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFS 360

Query: 361 AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI 420
           AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAV+FQNCNLYARKPNSNQRNI
Sbjct: 361 AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVIFQNCNLYARKPNSNQRNI 420

Query: 421 FTAQGREDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLV 480
           FTAQGREDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLV
Sbjct: 421 FTAQGREDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLV 480

Query: 481 EPVGWLEWSGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQG 540
           EPVGWLEWSGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQG
Sbjct: 481 EPVGWLEWSGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQG 540

Query: 541 SEWLNSTEIPFFSGLA 557
           SEWLNSTEIPFFSGLA
Sbjct: 541 SEWLNSTEIPFFSGLA 556

BLAST of Carg07156 vs. NCBI nr
Match: XP_023548978.1 (pectinesterase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1103.2 bits (2852), Expect = 0.0e+00
Identity = 547/556 (98.38%), Postives = 553/556 (99.46%), Query Frame = 0

Query: 1   MYGLRSRAQSSSNPTASISAIFTFFSVLFLCSFKPISSNPQTHQTARSLCNGTLFPNLCF 60
           MYGLR+RAQSSSNPTASISAIFTFFSVL LCSFKPISSNPQTHQTARSLCNGTLFPNLCF
Sbjct: 1   MYGLRNRAQSSSNPTASISAIFTFFSVLILCSFKPISSNPQTHQTARSLCNGTLFPNLCF 60

Query: 61  SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE 120
           SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGL+PTDRRALDDCLE
Sbjct: 61  SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLSPTDRRALDDCLE 120

Query: 121 LLDDSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGNVRKRVEL 180
           LLD SIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSG VRKRVEL
Sbjct: 121 LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL 180

Query: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE 240
           YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE
Sbjct: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE 240

Query: 241 TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGD 300
           TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNL+FVGD
Sbjct: 241 TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLVFVGD 300

Query: 301 GIGKTLIKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFS 360
           GIGKTLIKA+RNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFS
Sbjct: 301 GIGKTLIKANRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFS 360

Query: 361 AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI 420
           AFYQCSFIGYQDTLYVHSLRQFYRECDVYGT+DFIFGNAAVVFQNCNLYARKPNSNQRNI
Sbjct: 361 AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAVVFQNCNLYARKPNSNQRNI 420

Query: 421 FTAQGREDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLV 480
           FTAQGREDPNQNTGISILNCKVEAASDL+PVLPSFRTYLGRPWKLYSRTVFLKSFIGQLV
Sbjct: 421 FTAQGREDPNQNTGISILNCKVEAASDLIPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLV 480

Query: 481 EPVGWLEWSGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQG 540
           EPVGWLEWSGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQG
Sbjct: 481 EPVGWLEWSGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQG 540

Query: 541 SEWLNSTEIPFFSGLA 557
           SEWLNSTEIPFFSGLA
Sbjct: 541 SEWLNSTEIPFFSGLA 556

BLAST of Carg07156 vs. NCBI nr
Match: XP_022991224.1 (pectinesterase-like [Cucurbita maxima])

HSP 1 Score: 1101.3 bits (2847), Expect = 0.0e+00
Identity = 548/556 (98.56%), Postives = 551/556 (99.10%), Query Frame = 0

Query: 1   MYGLRSRAQSSSNPTASISAIFTFFSVLFLCSFKPISSNPQTHQTARSLCNGTLFPNLCF 60
           MYGLRSRAQSSSNPTASISAIFT FSVLFL SFKPISSNPQTHQTARSLCNGTLFPNLCF
Sbjct: 1   MYGLRSRAQSSSNPTASISAIFTLFSVLFLFSFKPISSNPQTHQTARSLCNGTLFPNLCF 60

Query: 61  SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE 120
           SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE
Sbjct: 61  SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE 120

Query: 121 LLDDSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGNVRKRVEL 180
           LLD SIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSG VRKRVEL
Sbjct: 121 LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL 180

Query: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE 240
           YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE
Sbjct: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE 240

Query: 241 TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGD 300
           TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGD
Sbjct: 241 TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGD 300

Query: 301 GIGKTLIKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFS 360
           GIGKTLIKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFS
Sbjct: 301 GIGKTLIKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFS 360

Query: 361 AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI 420
           AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI
Sbjct: 361 AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI 420

Query: 421 FTAQGREDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLV 480
           FTAQGREDPNQNTGISILNCKVEAASDL+PVL SFRTYLGRPWKLYSRT+FLKSFIGQLV
Sbjct: 421 FTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTIFLKSFIGQLV 480

Query: 481 EPVGWLEWSGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQG 540
           EPVGWLEW+GTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQG
Sbjct: 481 EPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQG 540

Query: 541 SEWLNSTEIPFFSGLA 557
           SEWLNSTEIPFFSGLA
Sbjct: 541 SEWLNSTEIPFFSGLA 556

BLAST of Carg07156 vs. ExPASy Swiss-Prot
Match: O81301 (Probable pectinesterase/pectinesterase inhibitor 40 OS=Arabidopsis thaliana OX=3702 GN=PME40 PE=2 SV=1)

HSP 1 Score: 547.0 bits (1408), Expect = 2.5e-154
Identity = 277/488 (56.76%), Postives = 353/488 (72.34%), Query Frame = 0

Query: 76  KLAGSALNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLELLDDSIAELQTSIYD 135
           +L  +ALN T   V+ + +N S L       L+  D  A DDCLELLDD++ +L T+I  
Sbjct: 39  ELVVTALNQTISNVNLSSSNFSDLLQRLGSNLSHRDLCAFDDCLELLDDTVFDLTTAI-- 98

Query: 136 LSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGN------------VRKRVELYLG 195
            S  +S +   H++  L+SAAMTN RTCLDGF +S  +            V + ++  L 
Sbjct: 99  -SKLRSHSPELHNVKMLLSAAMTNTRTCLDGFASSDNDENLNNNDNKTYGVAESLKESLF 158

Query: 196 KIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKF 255
            I  H+S  LAML+ IPG       E+V         GFPMWVS  DR LLQ  V+ETK 
Sbjct: 159 NISSHVSDSLAMLENIPGHIPGKVKEDV---------GFPMWVSGSDRNLLQDPVDETKV 218

Query: 256 NLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIG 315
           NLVVA++G+GN+TT+GEA++AAPNSS TRFVIYIK G YFEN+EI R+KT +MF+GDGIG
Sbjct: 219 NLVVAQNGTGNYTTIGEAISAAPNSSETRFVIYIKCGEYFENIEIPREKTMIMFIGDGIG 278

Query: 316 KTLIKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFSAFY 375
           +T+IKA+R+  DGWT F SATV   G+GFIAK ++F N+AGP KHQAVA RS+SD SA+Y
Sbjct: 279 RTVIKANRSYADGWTAFHSATVGVRGSGFIAKDLSFVNYAGPEKHQAVALRSSSDLSAYY 338

Query: 376 QCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTA 435
           +CSF  YQDT+YVHS +QFYRECD+YGT+DFIFG+A+VVFQNC+LYAR+PN NQ+ I+TA
Sbjct: 339 RCSFESYQDTIYVHSHKQFYRECDIYGTVDFIFGDASVVFQNCSLYARRPNPNQKIIYTA 398

Query: 436 QGREDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLVEPV 495
           QGRE+  + TGISI++ ++ AA DL+PV  +F+ YLGRPW+LYSRTV +KSFI  LV+P 
Sbjct: 399 QGRENSREPTGISIISSRILAAPDLIPVQANFKAYLGRPWQLYSRTVIMKSFIDDLVDPA 458

Query: 496 GWLEWSGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQGSEW 552
           GWL+W   FAL+TLYYGEY+N GPGSN T RV WPG++ I    EASQF+V  FI G++W
Sbjct: 459 GWLKWKDDFALETLYYGEYMNEGPGSNMTNRVQWPGFKRIETVEEASQFSVGPFIDGNKW 514

BLAST of Carg07156 vs. ExPASy Swiss-Prot
Match: O81415 (Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3702 GN=PME39 PE=2 SV=1)

HSP 1 Score: 518.8 bits (1335), Expect = 7.3e-146
Identity = 268/481 (55.72%), Postives = 338/481 (70.27%), Query Frame = 0

Query: 82  LNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLELLDDSIAELQTSIYDLSPSQS 141
           LN T  +V+ A +N S L+T  +  L   +R A +DCL LLDD+I++L+T++ DL   +S
Sbjct: 61  LNLTILKVNLASSNFSDLQTRLFPNLTHYERCAFEDCLGLLDDTISDLETAVSDL---RS 120

Query: 142 AARHSHDLLTLVSAAMTNHRTCLDGFYNSSG--------NVRKRVELYLGKIEQHLSIDL 201
           ++   +D+  L++  MT   TCLDGF  S           + + ++  +  I  +LS  L
Sbjct: 121 SSLEFNDISMLLTNVMTYQDTCLDGFSTSDNENNNDMTYELPENLKEIILDISNNLSNSL 180

Query: 202 AMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFNLVVAKDGSG 261
            ML+ I   K +  + EV  EY       P W+S  D+RLL+A V ET +NL VA DG+G
Sbjct: 181 HMLQVISRKKPSPKSSEVDVEY-------PSWLSENDQRLLEAPVQETNYNLSVAIDGTG 240

Query: 262 NFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTLIKADRNV 321
           NFTT+ +AV AAPN S TRF+IYIK G YFENVE+ +KKT +MF+GDGIGKT+IKA+R+ 
Sbjct: 241 NFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMFIGDGIGKTVIKANRSR 300

Query: 322 VDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFSAFYQCSFIGYQDT 381
           +DGW+TF++ TV   G G+IAK I+F N AGP+K QAVAFRS SD SAFY+C F GYQDT
Sbjct: 301 IDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVAFRSGSDHSAFYRCEFDGYQDT 360

Query: 382 LYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNT 441
           LYVHS +QFYRECD+YGTIDFIFGNAAVVFQN +LYARKPN   +  FTAQ R   +Q T
Sbjct: 361 LYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNPGHKIAFTAQSRNQSDQPT 420

Query: 442 GISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLVEPVGWLEWSGTFA 501
           GISILNC++ AA DL+PV  +F+ YLGRPW+ YSRTV +KSFI  L+ P GWLE    FA
Sbjct: 421 GISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSFIDDLIHPAGWLEGKKDFA 480

Query: 502 LDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQGSEWLNSTEIPFFS 555
           L+TLYYGEY+N GPG+N   RVTWPG+R I N TEA+QFTV  FI GS WLNST IPF  
Sbjct: 481 LETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGPFIDGSTWLNSTGIPFSL 531

BLAST of Carg07156 vs. ExPASy Swiss-Prot
Match: P85076 (Pectinesterase OS=Actinidia deliciosa OX=3627 PE=1 SV=1)

HSP 1 Score: 508.4 bits (1308), Expect = 9.9e-143
Identity = 245/319 (76.80%), Postives = 272/319 (85.27%), Query Frame = 0

Query: 237 AVNETKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLM 296
           AV +   ++VVAKDGSGNFTTVG AVAAA +SST RFVIYIK GAYFE V++++KKTNLM
Sbjct: 2   AVTDIVPDVVVAKDGSGNFTTVGAAVAAAKDSSTARFVIYIKEGAYFEYVDVDKKKTNLM 61

Query: 297 FVGDGIGKTLIKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSN 356
           F+GDGIGKT IK +R+VVDGWTTFRS+TVA VG GFIA+GI+FEN+AGPSKHQAVA RS 
Sbjct: 62  FIGDGIGKTWIKGNRSVVDGWTTFRSSTVAVVGTGFIARGISFENYAGPSKHQAVALRSG 121

Query: 357 SDFSAFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSN 416
           +DFSAFYQCSF+GYQDTLYVHSLRQFY ECDVYGTIDFIFGNAA V Q CNLYARKPN N
Sbjct: 122 ADFSAFYQCSFVGYQDTLYVHSLRQFYSECDVYGTIDFIFGNAAAVLQKCNLYARKPNEN 181

Query: 417 QRNIFTAQGREDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFI 476
           Q+NIFTAQGR+DPNQNTGISILNCKV AA+DL+PVL SF+TYLGRPWK YSRTVFL S +
Sbjct: 182 QKNIFTAQGRDDPNQNTGISILNCKVAAAADLIPVLSSFKTYLGRPWKEYSRTVFLLSQM 241

Query: 477 GQLVEPVGWLEWSGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEA 536
             L++P GWLEWSG FAL TLYY EY N GPGSNTT RVTWPGY V TN TE  QFTV  
Sbjct: 242 ESLIDPAGWLEWSGDFALTTLYYREYKNTGPGSNTTARVTWPGYAVTTNETEVIQFTVGN 301

Query: 537 FIQGSEWLNSTEIPFFSGL 556
           FIQGS+WL S  IP +  L
Sbjct: 302 FIQGSQWLTSYNIPVYLNL 320

BLAST of Carg07156 vs. ExPASy Swiss-Prot
Match: Q42534 (Pectinesterase 2 OS=Arabidopsis thaliana OX=3702 GN=PME2 PE=2 SV=2)

HSP 1 Score: 501.9 bits (1291), Expect = 9.3e-141
Identity = 274/529 (51.80%), Postives = 342/529 (64.65%), Query Frame = 0

Query: 42  THQTARSLCNGTLFPNLCFSKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKT 101
           +H   +S+C+ TL+P LCFS +++       S +++  ++LN T   V   Y    KL  
Sbjct: 58  SHAILKSVCSSTLYPELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKL-I 117

Query: 102 LFYGGLNPTDRRALDDCLELLDDSIAELQTSIYDLS--PSQSAAR-HSHDLLTLVSAAMT 161
               GL P +  AL DCLE +D+++ EL  ++ DL   P Q + R H+ DL TL+S+A+T
Sbjct: 118 AKRKGLTPREVTALHDCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAIT 177

Query: 162 NHRTCLDGFYNSSGNVRKRVELYLGK--IEQHLSIDLAMLKKIPGV---------KRAAT 221
           N  TCLDGF     + + R  L  G+  +E   S  LAM+K +            K +  
Sbjct: 178 NQGTCLDGFSYDDADRKVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTF 237

Query: 222 AEEVLPEYGAVRG-----GFPMWVSVKDRRLLQAAVNETKFNLVVAKDGSGNFTTVGEAV 281
                 +   V G     G+P W+SV DRRLLQ +    K +  VA DGSG+FTTV  AV
Sbjct: 238 TNNNNRKLKEVTGDLDSDGWPKWLSVGDRRLLQGST--IKADATVADDGSGDFTTVAAAV 297

Query: 282 AAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTLIKADRNVVDGWTTFRS 341
           AAAP  S  RFVI+IKAG Y ENVE+ +KKTN+MF+GDG GKT+I   RNVVDG TTF S
Sbjct: 298 AAAPEKSNKRFVIHIKAGVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHS 357

Query: 342 ATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFSAFYQCSFIGYQDTLYVHSLRQF 401
           ATVAAVG  F+A+ ITF+N AGPSKHQAVA R  SDFSAFYQC    YQDTLYVHS RQF
Sbjct: 358 ATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQF 417

Query: 402 YRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKV 461
           + +C + GT+DFIFGNAA V Q+C++ AR+PNS Q+N+ TAQGR DPNQNTGI I NC++
Sbjct: 418 FVKCHITGTVDFIFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRI 477

Query: 462 EAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLVEPVGWLEWSGTFALDTLYYGEY 521
              SDLL V  +F TYLGRPWK YSRTV ++S I  ++ P GW EWSG+FALDTL Y EY
Sbjct: 478 GGTSDLLAVKGTFPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREY 537

Query: 522 LNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQGSEWLNSTEIPF 552
           LNRG G+ T  RV W GY+VIT+ TEA  FT   FI G  WL ST  PF
Sbjct: 538 LNRGGGAGTANRVKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPF 583

BLAST of Carg07156 vs. ExPASy Swiss-Prot
Match: P83948 (Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1)

HSP 1 Score: 501.9 bits (1291), Expect = 9.3e-141
Identity = 267/530 (50.38%), Postives = 347/530 (65.47%), Query Frame = 0

Query: 37  SSNPQTHQTARSLCNGTLFPNLCFSKLSSFPQLAS--LSPEKLAGSALNFTNDEVHGAYA 96
           + N   H   +S C+ T +P+LCFS +++ P+ +    S + +   +LN T   V   Y 
Sbjct: 60  NGNEPHHAILKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYF 119

Query: 97  NCSKLKTLFYGGLNPTDRRALDDCLELLDDSIAELQTSIYDLSP---SQSAARHSHDLLT 156
              KL  L    L   ++ AL DCLE +D+++ EL  ++ DL      +S ++H+ DL T
Sbjct: 120 GIQKL--LKRTNLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKT 179

Query: 157 LVSAAMTNHRTCLDGFYNSSGNVRKRVELYLGK--IEQHLSIDLAMLKKIPG----VKRA 216
           L+SAAMTN  TCLDGF +   N   R  L  G+  +E+  S  LAM+K +      + R 
Sbjct: 180 LMSAAMTNQGTCLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRT 239

Query: 217 ATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFNLVVAKDGSGNFTTVGEAVAAA 276
           +   +++ E   V  G+P W+S  DRRLLQ+  +    N+VVA DGSGNF TV  +VAAA
Sbjct: 240 SNNRKLIEETSTV-DGWPAWLSTGDRRLLQS--SSVTPNVVVAADGSGNFKTVAASVAAA 299

Query: 277 PNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTLIKADRNVVDGWTTFRSATV 336
           P   T R++I IKAG Y ENVE+ +K  N+MF+GDG  +T+I   RNVVDG TTF+SATV
Sbjct: 300 PQGGTKRYIIRIKAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATV 359

Query: 337 AAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYRE 396
           A VG GF+A+ ITF+N AGPSKHQAVA R  +D SAFY C  + YQDTLYVHS RQF+  
Sbjct: 360 AVVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVN 419

Query: 397 CDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKVEAA 456
           C + GT+DFIFGNAA V QNC+++ARKPNS Q+N+ TAQGR DPNQNTGI I   ++ A 
Sbjct: 420 CLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRADPNQNTGIVIQKSRIGAT 479

Query: 457 SDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLVEPVGWLEWSGTFALDTLYYGEYLNR 516
           SDL PV  SF TYLGRPWK YSRTV ++S I  ++ P GW EW G FAL+TL+YGE+ N 
Sbjct: 480 SDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNA 539

Query: 517 GPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQGSEWLNSTEIPFFSGL 556
           G G+ T+GRV W G+RVIT++TEA  FT  +FI GS WL ST  PF  GL
Sbjct: 540 GAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

BLAST of Carg07156 vs. ExPASy TrEMBL
Match: A0A6J1GNU4 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111456156 PE=3 SV=1)

HSP 1 Score: 1107.8 bits (2864), Expect = 0.0e+00
Identity = 550/556 (98.92%), Postives = 552/556 (99.28%), Query Frame = 0

Query: 1   MYGLRSRAQSSSNPTASISAIFTFFSVLFLCSFKPISSNPQTHQTARSLCNGTLFPNLCF 60
           MYGLRSRAQSSSNPTASISAIFTFFSVLFLCSFKPISSNPQTHQTARSLCNGTLFPNLCF
Sbjct: 1   MYGLRSRAQSSSNPTASISAIFTFFSVLFLCSFKPISSNPQTHQTARSLCNGTLFPNLCF 60

Query: 61  SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE 120
           SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE
Sbjct: 61  SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE 120

Query: 121 LLDDSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGNVRKRVEL 180
           LLD SIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSG VRKRVEL
Sbjct: 121 LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL 180

Query: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE 240
           YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQA+VNE
Sbjct: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQASVNE 240

Query: 241 TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGD 300
           TKFNLVVAKDGSGNFTTVG AVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGD
Sbjct: 241 TKFNLVVAKDGSGNFTTVGAAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGD 300

Query: 301 GIGKTLIKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFS 360
           GIGKTLIKADRNVVDGWTTFRSATV AVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFS
Sbjct: 301 GIGKTLIKADRNVVDGWTTFRSATVVAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFS 360

Query: 361 AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI 420
           AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAV+FQNCNLYARKPNSNQRNI
Sbjct: 361 AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVIFQNCNLYARKPNSNQRNI 420

Query: 421 FTAQGREDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLV 480
           FTAQGREDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLV
Sbjct: 421 FTAQGREDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLV 480

Query: 481 EPVGWLEWSGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQG 540
           EPVGWLEWSGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQG
Sbjct: 481 EPVGWLEWSGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQG 540

Query: 541 SEWLNSTEIPFFSGLA 557
           SEWLNSTEIPFFSGLA
Sbjct: 541 SEWLNSTEIPFFSGLA 556

BLAST of Carg07156 vs. ExPASy TrEMBL
Match: A0A6J1JU83 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111487942 PE=3 SV=1)

HSP 1 Score: 1101.3 bits (2847), Expect = 0.0e+00
Identity = 548/556 (98.56%), Postives = 551/556 (99.10%), Query Frame = 0

Query: 1   MYGLRSRAQSSSNPTASISAIFTFFSVLFLCSFKPISSNPQTHQTARSLCNGTLFPNLCF 60
           MYGLRSRAQSSSNPTASISAIFT FSVLFL SFKPISSNPQTHQTARSLCNGTLFPNLCF
Sbjct: 1   MYGLRSRAQSSSNPTASISAIFTLFSVLFLFSFKPISSNPQTHQTARSLCNGTLFPNLCF 60

Query: 61  SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE 120
           SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE
Sbjct: 61  SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE 120

Query: 121 LLDDSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGNVRKRVEL 180
           LLD SIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSG VRKRVEL
Sbjct: 121 LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL 180

Query: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE 240
           YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE
Sbjct: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE 240

Query: 241 TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGD 300
           TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGD
Sbjct: 241 TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGD 300

Query: 301 GIGKTLIKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFS 360
           GIGKTLIKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFS
Sbjct: 301 GIGKTLIKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFS 360

Query: 361 AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI 420
           AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI
Sbjct: 361 AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI 420

Query: 421 FTAQGREDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLV 480
           FTAQGREDPNQNTGISILNCKVEAASDL+PVL SFRTYLGRPWKLYSRT+FLKSFIGQLV
Sbjct: 421 FTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTIFLKSFIGQLV 480

Query: 481 EPVGWLEWSGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQG 540
           EPVGWLEW+GTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQG
Sbjct: 481 EPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQG 540

Query: 541 SEWLNSTEIPFFSGLA 557
           SEWLNSTEIPFFSGLA
Sbjct: 541 SEWLNSTEIPFFSGLA 556

BLAST of Carg07156 vs. ExPASy TrEMBL
Match: A0A1S3BSU3 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492792 PE=3 SV=1)

HSP 1 Score: 1000.7 bits (2586), Expect = 2.3e-288
Identity = 501/555 (90.27%), Postives = 515/555 (92.79%), Query Frame = 0

Query: 1   MYGLRSRAQSSSNPTASISAIFTFFSVLFLCSFKPISSNPQTHQTARSLCNGTLFPNLCF 60
           MYGLR+RAQ SSNPT SIS I TFF + FL S KPISSNPQ HQTARSLCNGTLFPNLCF
Sbjct: 1   MYGLRNRAQISSNPT-SISTISTFFFIFFLFSSKPISSNPQIHQTARSLCNGTLFPNLCF 60

Query: 61  SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE 120
           SKLSSFPQLASLSPEKLAGSALNFT  EV  AYANC+ LKT  Y GLNPTDR ALDDCLE
Sbjct: 61  SKLSSFPQLASLSPEKLAGSALNFTTREVLLAYANCTNLKTHLYCGLNPTDRHALDDCLE 120

Query: 121 LLDDSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGNVRKRVEL 180
           LLDDSIAEL+ SI DL PSQSAARHSHDLLTLVSAAMTNHRTC+DGFYNSSG VR RVEL
Sbjct: 121 LLDDSIAELKASILDLGPSQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVEL 180

Query: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE 240
           YLGKI QHLSIDLAMLKKIPGV RA   EEVLPEYGAV+GGFP WVSVKDRRLLQAAVNE
Sbjct: 181 YLGKIGQHLSIDLAMLKKIPGVNRATKVEEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNE 240

Query: 241 TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGD 300
           TKFNLVVAKDGSGNFTTV EAVAAAPNSSTTRFVIYIKAGAYFENVEI R K+NLMFVGD
Sbjct: 241 TKFNLVVAKDGSGNFTTVNEAVAAAPNSSTTRFVIYIKAGAYFENVEIGRAKSNLMFVGD 300

Query: 301 GIGKTLIKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFS 360
           GIGKTLIK DRNVVDGWTTFRSATVA VG GFIAKGITFEN+AGPSKHQAVA RSNSDFS
Sbjct: 301 GIGKTLIKGDRNVVDGWTTFRSATVAVVGTGFIAKGITFENYAGPSKHQAVALRSNSDFS 360

Query: 361 AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI 420
           AFYQCSFIGYQDTLYVHSLRQFYRECD+YGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI
Sbjct: 361 AFYQCSFIGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI 420

Query: 421 FTAQGREDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLV 480
           FTAQGREDPNQNTGISILNCKVEAASDL+PVL SFRTYLGRPWKL+SRTVFL+SFIGQLV
Sbjct: 421 FTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLHSRTVFLRSFIGQLV 480

Query: 481 EPVGWLEWSGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQG 540
           EPVGWLEW+GTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITN+TEASQFTVE FIQG
Sbjct: 481 EPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNATEASQFTVEEFIQG 540

Query: 541 SEWLNSTEIPFFSGL 556
           S WLNST IPFFSGL
Sbjct: 541 SSWLNSTGIPFFSGL 554

BLAST of Carg07156 vs. ExPASy TrEMBL
Match: A0A5D3D1W6 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G001300 PE=3 SV=1)

HSP 1 Score: 1000.3 bits (2585), Expect = 3.1e-288
Identity = 500/555 (90.09%), Postives = 515/555 (92.79%), Query Frame = 0

Query: 1   MYGLRSRAQSSSNPTASISAIFTFFSVLFLCSFKPISSNPQTHQTARSLCNGTLFPNLCF 60
           MYGLR+RAQ SSNPT SIS I TFF + FL S KPISSNPQ HQTARSLCNGTLFPNLCF
Sbjct: 1   MYGLRNRAQISSNPT-SISTISTFFFIFFLFSSKPISSNPQIHQTARSLCNGTLFPNLCF 60

Query: 61  SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE 120
           SKLSSFPQLASLSPEKLAGSALNFT  EV  AYANC+ LKT  Y GLNPTDR ALDDCLE
Sbjct: 61  SKLSSFPQLASLSPEKLAGSALNFTTREVLLAYANCTNLKTHLYCGLNPTDRHALDDCLE 120

Query: 121 LLDDSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGNVRKRVEL 180
           LLDDSIAEL+ SI DL PSQSAARHSHDLLTLVSAAMTNHRTC+DGFYNSSG VR RVEL
Sbjct: 121 LLDDSIAELKASILDLGPSQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVEL 180

Query: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE 240
           YLGKI QHLSIDLAMLKKIPGV RA   EEVLPEYGAV+GGFP WVSVKDRRLLQAAVNE
Sbjct: 181 YLGKIGQHLSIDLAMLKKIPGVNRATKVEEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNE 240

Query: 241 TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGD 300
           TKFNLVVAKDGSGNFTTV EAVAAAPNSSTTRFVIYIKAGAYFENVEI R K+NLMFVGD
Sbjct: 241 TKFNLVVAKDGSGNFTTVNEAVAAAPNSSTTRFVIYIKAGAYFENVEIGRAKSNLMFVGD 300

Query: 301 GIGKTLIKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFS 360
           GIGKTLIK DRNVVDGWTTFRSATVA +G GFIAKGITFEN+AGPSKHQAVA RSNSDFS
Sbjct: 301 GIGKTLIKGDRNVVDGWTTFRSATVAVIGTGFIAKGITFENYAGPSKHQAVALRSNSDFS 360

Query: 361 AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI 420
           AFYQCSFIGYQDTLYVHSLRQFYRECD+YGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI
Sbjct: 361 AFYQCSFIGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI 420

Query: 421 FTAQGREDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLV 480
           FTAQGREDPNQNTGISILNCKVEAASDL+PVL SFRTYLGRPWKL+SRTVFL+SFIGQLV
Sbjct: 421 FTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLHSRTVFLRSFIGQLV 480

Query: 481 EPVGWLEWSGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQG 540
           EPVGWLEW+GTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITN+TEASQFTVE FIQG
Sbjct: 481 EPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNATEASQFTVEEFIQG 540

Query: 541 SEWLNSTEIPFFSGL 556
           S WLNST IPFFSGL
Sbjct: 541 SSWLNSTGIPFFSGL 554

BLAST of Carg07156 vs. ExPASy TrEMBL
Match: A0A0A0K5K8 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G405320 PE=3 SV=1)

HSP 1 Score: 934.9 bits (2415), Expect = 1.6e-268
Identity = 475/561 (84.67%), Postives = 502/561 (89.48%), Query Frame = 0

Query: 6   SRAQSSSNPTASISAIFTFFSVLFLCSFKPISSNP---------QT-HQTARSLCNGTLF 65
           S + SSS+ ++S S+  +  S     S    SS+P         QT HQTARSLCNGTLF
Sbjct: 51  SSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSPPPPPPLPLFQTFHQTARSLCNGTLF 110

Query: 66  PNLCFSKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRAL 125
           P+LCFSKLSSFPQLASLSPEKLAGSALNFT+ EV  AY NC+ LKT  Y GLNPTDR AL
Sbjct: 111 PDLCFSKLSSFPQLASLSPEKLAGSALNFTSREVLLAYTNCTNLKTHLYSGLNPTDRHAL 170

Query: 126 DDCLELLDDSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGNVR 185
           DDCLELLD SIAEL+ SI+DL+PSQS A HSHDLLTLVSAAMTNHRTC+DGFYNSSG VR
Sbjct: 171 DDCLELLDGSIAELKASIFDLAPSQSPALHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVR 230

Query: 186 KRVELYLGKIEQHLSIDLAMLKKIPGVKRA-ATAEEVLPEYGAVRGGFPMWVSVKDRRLL 245
            RVELYLGKI QHLSIDLAMLKKIPGV RA    +E+LPEYGAV+GGFP WVSVKDRRLL
Sbjct: 231 SRVELYLGKIGQHLSIDLAMLKKIPGVNRATGVDQEMLPEYGAVKGGFPKWVSVKDRRLL 290

Query: 246 QAAVNETKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTN 305
           QAAVNETKFN+VVAKDGSGNFTTV EAVAAAPN+STTRFVIYIKAGAYFENVEI R K+N
Sbjct: 291 QAAVNETKFNMVVAKDGSGNFTTVSEAVAAAPNASTTRFVIYIKAGAYFENVEIGRAKSN 350

Query: 306 LMFVGDGIGKTLIKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFR 365
           LMFVGDGIGKTLIKADRNVVDGWTTFRSATVA VG GFIAKGITFEN+AGPSKHQAVA R
Sbjct: 351 LMFVGDGIGKTLIKADRNVVDGWTTFRSATVAVVGTGFIAKGITFENYAGPSKHQAVALR 410

Query: 366 SNSDFSAFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPN 425
           SNSDFSAFYQCSFIGYQDTLYVHSLRQFYR+CDVYGTIDFIFGNAAVVFQNCNLYARKPN
Sbjct: 411 SNSDFSAFYQCSFIGYQDTLYVHSLRQFYRDCDVYGTIDFIFGNAAVVFQNCNLYARKPN 470

Query: 426 SNQRNIFTAQGREDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKS 485
           SNQRNIFTAQGREDPNQNTGISILNCKVEAASDL+PVL SFRTYLGRPWKLYSRTVFL+S
Sbjct: 471 SNQRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRS 530

Query: 486 FIGQLVEPVGWLEWSGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTV 545
           FIGQL+EPVGWLEW+GTFALDTLYYGEYLNRGPGSNTT RVTWPGYRVITN+TEASQFTV
Sbjct: 531 FIGQLIEPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTMRVTWPGYRVITNATEASQFTV 590

Query: 546 EAFIQGSEWLNSTEIPFFSGL 556
           E FIQGS WLNSTEIPFFSGL
Sbjct: 591 ERFIQGSSWLNSTEIPFFSGL 611

BLAST of Carg07156 vs. TAIR 10
Match: AT4G02320.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 547.0 bits (1408), Expect = 1.8e-155
Identity = 277/488 (56.76%), Postives = 353/488 (72.34%), Query Frame = 0

Query: 76  KLAGSALNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLELLDDSIAELQTSIYD 135
           +L  +ALN T   V+ + +N S L       L+  D  A DDCLELLDD++ +L T+I  
Sbjct: 39  ELVVTALNQTISNVNLSSSNFSDLLQRLGSNLSHRDLCAFDDCLELLDDTVFDLTTAI-- 98

Query: 136 LSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGN------------VRKRVELYLG 195
            S  +S +   H++  L+SAAMTN RTCLDGF +S  +            V + ++  L 
Sbjct: 99  -SKLRSHSPELHNVKMLLSAAMTNTRTCLDGFASSDNDENLNNNDNKTYGVAESLKESLF 158

Query: 196 KIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKF 255
            I  H+S  LAML+ IPG       E+V         GFPMWVS  DR LLQ  V+ETK 
Sbjct: 159 NISSHVSDSLAMLENIPGHIPGKVKEDV---------GFPMWVSGSDRNLLQDPVDETKV 218

Query: 256 NLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIG 315
           NLVVA++G+GN+TT+GEA++AAPNSS TRFVIYIK G YFEN+EI R+KT +MF+GDGIG
Sbjct: 219 NLVVAQNGTGNYTTIGEAISAAPNSSETRFVIYIKCGEYFENIEIPREKTMIMFIGDGIG 278

Query: 316 KTLIKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFSAFY 375
           +T+IKA+R+  DGWT F SATV   G+GFIAK ++F N+AGP KHQAVA RS+SD SA+Y
Sbjct: 279 RTVIKANRSYADGWTAFHSATVGVRGSGFIAKDLSFVNYAGPEKHQAVALRSSSDLSAYY 338

Query: 376 QCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTA 435
           +CSF  YQDT+YVHS +QFYRECD+YGT+DFIFG+A+VVFQNC+LYAR+PN NQ+ I+TA
Sbjct: 339 RCSFESYQDTIYVHSHKQFYRECDIYGTVDFIFGDASVVFQNCSLYARRPNPNQKIIYTA 398

Query: 436 QGREDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLVEPV 495
           QGRE+  + TGISI++ ++ AA DL+PV  +F+ YLGRPW+LYSRTV +KSFI  LV+P 
Sbjct: 399 QGRENSREPTGISIISSRILAAPDLIPVQANFKAYLGRPWQLYSRTVIMKSFIDDLVDPA 458

Query: 496 GWLEWSGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQGSEW 552
           GWL+W   FAL+TLYYGEY+N GPGSN T RV WPG++ I    EASQF+V  FI G++W
Sbjct: 459 GWLKWKDDFALETLYYGEYMNEGPGSNMTNRVQWPGFKRIETVEEASQFSVGPFIDGNKW 514

BLAST of Carg07156 vs. TAIR 10
Match: AT4G02300.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 518.8 bits (1335), Expect = 5.2e-147
Identity = 268/481 (55.72%), Postives = 338/481 (70.27%), Query Frame = 0

Query: 82  LNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLELLDDSIAELQTSIYDLSPSQS 141
           LN T  +V+ A +N S L+T  +  L   +R A +DCL LLDD+I++L+T++ DL   +S
Sbjct: 61  LNLTILKVNLASSNFSDLQTRLFPNLTHYERCAFEDCLGLLDDTISDLETAVSDL---RS 120

Query: 142 AARHSHDLLTLVSAAMTNHRTCLDGFYNSSG--------NVRKRVELYLGKIEQHLSIDL 201
           ++   +D+  L++  MT   TCLDGF  S           + + ++  +  I  +LS  L
Sbjct: 121 SSLEFNDISMLLTNVMTYQDTCLDGFSTSDNENNNDMTYELPENLKEIILDISNNLSNSL 180

Query: 202 AMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFNLVVAKDGSG 261
            ML+ I   K +  + EV  EY       P W+S  D+RLL+A V ET +NL VA DG+G
Sbjct: 181 HMLQVISRKKPSPKSSEVDVEY-------PSWLSENDQRLLEAPVQETNYNLSVAIDGTG 240

Query: 262 NFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTLIKADRNV 321
           NFTT+ +AV AAPN S TRF+IYIK G YFENVE+ +KKT +MF+GDGIGKT+IKA+R+ 
Sbjct: 241 NFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMFIGDGIGKTVIKANRSR 300

Query: 322 VDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFSAFYQCSFIGYQDT 381
           +DGW+TF++ TV   G G+IAK I+F N AGP+K QAVAFRS SD SAFY+C F GYQDT
Sbjct: 301 IDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVAFRSGSDHSAFYRCEFDGYQDT 360

Query: 382 LYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNT 441
           LYVHS +QFYRECD+YGTIDFIFGNAAVVFQN +LYARKPN   +  FTAQ R   +Q T
Sbjct: 361 LYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNPGHKIAFTAQSRNQSDQPT 420

Query: 442 GISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLVEPVGWLEWSGTFA 501
           GISILNC++ AA DL+PV  +F+ YLGRPW+ YSRTV +KSFI  L+ P GWLE    FA
Sbjct: 421 GISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSFIDDLIHPAGWLEGKKDFA 480

Query: 502 LDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQGSEWLNSTEIPFFS 555
           L+TLYYGEY+N GPG+N   RVTWPG+R I N TEA+QFTV  FI GS WLNST IPF  
Sbjct: 481 LETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGPFIDGSTWLNSTGIPFSL 531

BLAST of Carg07156 vs. TAIR 10
Match: AT1G53830.1 (pectin methylesterase 2 )

HSP 1 Score: 501.9 bits (1291), Expect = 6.6e-142
Identity = 274/529 (51.80%), Postives = 342/529 (64.65%), Query Frame = 0

Query: 42  THQTARSLCNGTLFPNLCFSKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKT 101
           +H   +S+C+ TL+P LCFS +++       S +++  ++LN T   V   Y    KL  
Sbjct: 58  SHAILKSVCSSTLYPELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKL-I 117

Query: 102 LFYGGLNPTDRRALDDCLELLDDSIAELQTSIYDLS--PSQSAAR-HSHDLLTLVSAAMT 161
               GL P +  AL DCLE +D+++ EL  ++ DL   P Q + R H+ DL TL+S+A+T
Sbjct: 118 AKRKGLTPREVTALHDCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAIT 177

Query: 162 NHRTCLDGFYNSSGNVRKRVELYLGK--IEQHLSIDLAMLKKIPGV---------KRAAT 221
           N  TCLDGF     + + R  L  G+  +E   S  LAM+K +            K +  
Sbjct: 178 NQGTCLDGFSYDDADRKVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTF 237

Query: 222 AEEVLPEYGAVRG-----GFPMWVSVKDRRLLQAAVNETKFNLVVAKDGSGNFTTVGEAV 281
                 +   V G     G+P W+SV DRRLLQ +    K +  VA DGSG+FTTV  AV
Sbjct: 238 TNNNNRKLKEVTGDLDSDGWPKWLSVGDRRLLQGST--IKADATVADDGSGDFTTVAAAV 297

Query: 282 AAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTLIKADRNVVDGWTTFRS 341
           AAAP  S  RFVI+IKAG Y ENVE+ +KKTN+MF+GDG GKT+I   RNVVDG TTF S
Sbjct: 298 AAAPEKSNKRFVIHIKAGVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHS 357

Query: 342 ATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFSAFYQCSFIGYQDTLYVHSLRQF 401
           ATVAAVG  F+A+ ITF+N AGPSKHQAVA R  SDFSAFYQC    YQDTLYVHS RQF
Sbjct: 358 ATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQF 417

Query: 402 YRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKV 461
           + +C + GT+DFIFGNAA V Q+C++ AR+PNS Q+N+ TAQGR DPNQNTGI I NC++
Sbjct: 418 FVKCHITGTVDFIFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRI 477

Query: 462 EAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLVEPVGWLEWSGTFALDTLYYGEY 521
              SDLL V  +F TYLGRPWK YSRTV ++S I  ++ P GW EWSG+FALDTL Y EY
Sbjct: 478 GGTSDLLAVKGTFPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREY 537

Query: 522 LNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQGSEWLNSTEIPF 552
           LNRG G+ T  RV W GY+VIT+ TEA  FT   FI G  WL ST  PF
Sbjct: 538 LNRGGGAGTANRVKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPF 583

BLAST of Carg07156 vs. TAIR 10
Match: AT3G14310.1 (pectin methylesterase 3 )

HSP 1 Score: 477.2 bits (1227), Expect = 1.7e-134
Identity = 260/544 (47.79%), Postives = 339/544 (62.32%), Query Frame = 0

Query: 39  NPQTHQTARSLCNGTLFPNLCFSKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSK 98
           +P +H   RS C+ T +P LC S + +   +   S + +  +++N T   V   Y    K
Sbjct: 52  SPSSHAVLRSSCSSTRYPELCISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKK 111

Query: 99  LKTLFYGGLNPTDRRALDDCLELLDDSIAELQTSIYDLS--PSQSAAR-HSHDLLTLVSA 158
           L      GL P ++ AL DCLE +D+++ EL  ++ DL   P++   R H+ DL TL+S+
Sbjct: 112 L-IKKRKGLTPREKTALHDCLETIDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISS 171

Query: 159 AMTNHRTCLDGFYNSSGNVRKRVELYLGKI--EQHLSIDLAMLKKIPGV----------- 218
           A+TN  TCLDGF +   + + R  L  G+I  E   S  LAM+K +              
Sbjct: 172 AITNQETCLDGFSHDDADKQVRKALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKI 231

Query: 219 -----------KRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFNLVVAKDG 278
                      +    A ++         G+P W+S  DRRLLQ   +  K +  VA DG
Sbjct: 232 TSNNRKLKEENQETTVAVDIAGAGELDSEGWPTWLSAGDRRLLQG--SGVKADATVAADG 291

Query: 279 SGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGDGIGKTLIKADR 338
           SG F TV  AVAAAP +S  R+VI+IKAG Y ENVE+ +KK N+MF+GDG  +T+I   R
Sbjct: 292 SGTFKTVAAAVAAAPENSNKRYVIHIKAGVYRENVEVAKKKKNIMFMGDGRTRTIITGSR 351

Query: 339 NVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFSAFYQCSFIGYQ 398
           NVVDG TTF SATVAAVG  F+A+ ITF+N AGPSKHQAVA R  SDFSAFY C  + YQ
Sbjct: 352 NVVDGSTTFHSATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQ 411

Query: 399 DTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQ 458
           DTLYVHS RQF+ +C + GT+DFIFGNAAVV Q+C+++AR+PNS Q+N+ TAQGR DPNQ
Sbjct: 412 DTLYVHSNRQFFVKCLIAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQ 471

Query: 459 NTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLVEPVGWLEWSGT 518
           NTGI I  C++ A SDL  V  SF TYLGRPWK YS+TV ++S I  ++ P GW EW+GT
Sbjct: 472 NTGIVIQKCRIGATSDLQSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGT 531

Query: 519 FALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQGSEWLNSTEIPF 556
           FAL+TL Y EY N G G+ T  RV W G++VIT + EA ++T   FI G  WL+ST  PF
Sbjct: 532 FALNTLTYREYSNTGAGAGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPF 591

BLAST of Carg07156 vs. TAIR 10
Match: AT3G49220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 472.2 bits (1214), Expect = 5.6e-133
Identity = 257/526 (48.86%), Postives = 336/526 (63.88%), Query Frame = 0

Query: 44  QTARSLCNGTLFPNLCFSKLSSFP-QLASLSPEKLAGSALNFT-NDEVHGAYANCSKLKT 103
           Q     C  T FP LC   L  FP  LA+ S + L    +N T +   H  Y++ S    
Sbjct: 85  QAISKACELTRFPELCVDSLMDFPGSLAASSSKDLIHVTVNMTLHHFSHALYSSAS---- 144

Query: 104 LFYGGLNPTDRRALDDCLELLDDSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHR 163
           L +  + P  R A D C+ELLDDS+  L  +   LS   S++    D+ T +SAA+TNH 
Sbjct: 145 LSFVDMPPRARSAYDSCVELLDDSVDALSRA---LSSVVSSSAKPQDVTTWLSAALTNHD 204

Query: 164 TCLDGFYN-SSGNVRKRVELYLGKIEQHLSIDLAMLKK---------IPGVKRAATAEEV 223
           TC +GF     G V+  +   L  + + +S  LA+            +P   R     E 
Sbjct: 205 TCTEGFDGVDDGGVKDHMTAALQNLSELVSNCLAIFSASHDGDDFAGVPIQNRRLLGVEE 264

Query: 224 LPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFNLVVAKDGSGNFTTVGEAVAAAPNSSTT 283
             E       FP W+  K+R +L+  V++ + +++V+KDG+G   T+ EA+  AP +ST 
Sbjct: 265 REE------KFPRWMRPKEREILEMPVSQIQADIIVSKDGNGTCKTISEAIKKAPQNSTR 324

Query: 284 RFVIYIKAGAYFE-NVEIERKKTNLMFVGDGIGKTLIKADRNVVDGWTTFRSATVAAVGN 343
           R +IY+KAG Y E N+++ RKK NLMFVGDG GKT+I   +++ D  TTF +A+ AA G 
Sbjct: 325 RIIIYVKAGRYEENNLKVGRKKINLMFVGDGKGKTVISGGKSIFDNITTFHTASFAATGA 384

Query: 344 GFIAKGITFENFAGPSKHQAVAFRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYRECDVYG 403
           GFIA+ ITFEN+AGP+KHQAVA R  +D +  Y+C+ IGYQDTLYVHS RQF+RECD+YG
Sbjct: 385 GFIARDITFENWAGPAKHQAVALRIGADHAVIYRCNIIGYQDTLYVHSNRQFFRECDIYG 444

Query: 404 TIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKVEAASDLLP 463
           T+DFIFGNAAVV QNC++YARKP   Q+N  TAQ R+DPNQNTGISI   +V AASDL  
Sbjct: 445 TVDFIFGNAAVVLQNCSIYARKPMDFQKNTITAQNRKDPNQNTGISIHASRVLAASDLQA 504

Query: 464 VLPSFRTYLGRPWKLYSRTVFLKSFIGQLVEPVGWLEWSGTFALDTLYYGEYLNRGPGSN 523
              S +TYLGRPWKL+SRTV++ S+IG  V   GWLEW+ TFALDTLYYGEYLN GPGS 
Sbjct: 505 TNGSTQTYLGRPWKLFSRTVYMMSYIGGHVHTRGWLEWNTTFALDTLYYGEYLNSGPGSG 564

Query: 524 TTGRVTWPGYRVITNSTEASQFTVEAFIQGSEWLNSTEIPFFSGLA 557
              RV+WPGYRVI ++ EA++FTV  FI GS WL ST + F +GL+
Sbjct: 565 LGQRVSWPGYRVINSTAEANRFTVAEFIYGSSWLPSTGVSFLAGLS 597

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7014347.10.0e+00100.00putative pectinesterase/pectinesterase inhibitor 40 [Cucurbita argyrosperma subs... [more]
KAG6575805.10.0e+0099.10putative pectinesterase/pectinesterase inhibitor 40, partial [Cucurbita argyrosp... [more]
XP_022953706.10.0e+0098.92pectinesterase-like [Cucurbita moschata][more]
XP_023548978.10.0e+0098.38pectinesterase-like [Cucurbita pepo subsp. pepo][more]
XP_022991224.10.0e+0098.56pectinesterase-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
O813012.5e-15456.76Probable pectinesterase/pectinesterase inhibitor 40 OS=Arabidopsis thaliana OX=3... [more]
O814157.3e-14655.72Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3... [more]
P850769.9e-14376.80Pectinesterase OS=Actinidia deliciosa OX=3627 PE=1 SV=1[more]
Q425349.3e-14151.80Pectinesterase 2 OS=Arabidopsis thaliana OX=3702 GN=PME2 PE=2 SV=2[more]
P839489.3e-14150.38Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1GNU40.0e+0098.92Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111456156 PE=3 SV=1[more]
A0A6J1JU830.0e+0098.56Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111487942 PE=3 SV=1[more]
A0A1S3BSU32.3e-28890.27Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492792 PE=3 SV=1[more]
A0A5D3D1W63.1e-28890.09Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G00130... [more]
A0A0A0K5K81.6e-26884.67Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G405320 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G02320.11.8e-15556.76Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G02300.15.2e-14755.72Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT1G53830.16.6e-14251.80pectin methylesterase 2 [more]
AT3G14310.11.7e-13447.79pectin methylesterase 3 [more]
AT3G49220.15.6e-13348.86Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 41..195
e-value: 3.6E-31
score: 119.6
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 20..198
e-value: 1.2E-25
score: 87.8
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 47..194
e-value: 9.2E-25
score: 87.7
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 244..540
e-value: 1.7E-145
score: 483.4
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 44..201
e-value: 7.2E-29
score: 102.7
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 47..200
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 238..555
e-value: 1.1E-122
score: 411.5
NoneNo IPR availablePANTHERPTHR31707:SF257PECTINESTERASEcoord: 40..552
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 40..552
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 48..201
e-value: 5.12383E-28
score: 107.524
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 388..397
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 242..554

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg07156-RACarg07156-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity