Carg07155 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg07155
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionPectinesterase
LocationCarg_Chr17: 8052657 .. 8055095 (-)
RNA-Seq ExpressionCarg07155
SyntenyCarg07155
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGCTGCCCCACCCACCACCGCCGCATTCTACTCTCTCCTCCTCATCTCCCTCTACATCACCGCCGCTGGAATCTCCACCTCCGCCGCTCCCACCGGTTCGGTGTGCAGCTCGGCCCCAGACCCCTCCTACTGCAAATCGGCCCTCCCAAACCAAACCGGCAATGTGTACTCGTACGGACGGTCGTCCGTCAGAAAGTCGCTGTCGTCGTCGCAGAAGTTCCTCCGGCTAGTGGAGAAGTATCTCCGGTCCCGGTCGTCGCTGACGGTGGCGGCAGTTAGGGCGCTGGAGGACTGCCAGCTGCTGGCGGGACTGAACATAGATTACCTGACGACGTCGTTCCGGACGGTGAACTCGACGAGCTGGACGCTGCCGGAGATGAAGGCGGAGGACGTGCAGAGCCTGTTGAGTGCGATATTGACGAACCAACAGACATGTTTGGATGGGCTTAAGGCGACGGCGGGGTCTTGGTCTCTCAAAAATGGCCTTTCCCTCCCACTTGCCGCTGACACCAAGCTCTACTCTATGTCCTTAGCCTTTTTCACCAAAGGTTTGCGTTTATTTTATTAATATCCTTTTATTATTATTATTATTGAAAATTATCCACTAATTTGGAGGAGGATCATGAGTTTATAAATAAAAAAAATACATTTTCATTGACACCAAACATTTTAGATAAGCCTAAACAAAGTCACGAGAGCTTATGTTCAAAGTGGACAATATCATACCTACCATTGTGGAGAATCGTGATTCCTAACATGGTATTAGAGTCATGTCCTTAAATTAGCCATGTCAATAGAATTCTTTGTTTAAGAACTCCAGAGGAGTCAAGCCTCAATTCTGTTCGAAAGCTTTGTAGGTTTCAAAAGAGACTCTATAGTGTACAATTATTATACCATTGTGAAAAGTCGTGATTTCTAACAATAATAATTATTATTTAATAGATGACAAAGTCGTGATTTCTAACAATAATAATTATTATTTAATAGATGAGAGCTTCGTAGGCTCCAGGAGATTCTTAACTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTTAACATTAATTATTATTAGTTAACATTATTGATTTTTTATTCTCCACAGGTTGGGTACCAAAAAAGAAGAAAAAACCGACATGGACTTCAGCCGGGAGACAGGGCGGGTTCAGCAACGGACGAATGTCACTCAAAATGTCCAGCAGAACCAAAGCGATTTACGAGACGGCAACTCGCCGGAATCTGCTGCAGACTGACGACGGTGGCGACGATCAGATTTCGGTTAGGGACATCGTCGTCGTCAGTCAAGACGGCAGCGGAAATTTCACAACCATTAATGAAGCGATTGCGGCGGCGCCGAACAACTCTGCTCCGACGGACGGCTATTTCTTGATCTTCGTGGATGCTGGAGTTTATGAAGAATACGTGACGATTGCGAAGAATAAGAGGTACTTGATGATGATCGGAGATGGGATTAATCAGACCGTCGTCACTGGTAATCGGAACGTCGTCGACGGCTGGACCACTTTTAATTCCGCCACATTTGGTAAGTGTTTCCACAATTTAAAATTTGTTATATAATAAATTAATAAAGAATTTTAATAATTTAATAGAAAAATTAAATAGGAAAGACATTTTTTAAGCAAATATAAAAATTATTAACAATTAAAGTAAAATAAAAGGTTTATCAAACCGGGTCTCATTTACCCGTTTTCGTGTCATCACCAGCGGTGGTCGGACCCGGATTCGTAGCAGTAAACATGACATTCAGAAACACCGCCGGAGCAATAAAACACCAAGCCGTGGCCGTCCGGAACAGCGCCGACTTATCAACATTCTACCTCTGCAGCTTCGAAGGCTACCAAGACACCTTATACACCCACTCCCTCCGCCAATTCTACCGTGACTGCGATATTTACGGCACCGTCGACTTCATCTTCGGCAACGCCGCCGTCGTCTTACAGAACTGCAACATCTACCCGCGTCTGCCGATGGCCAACCAGTTCAACGCCATCACTGCCCAAGGCCGGACCGACCCAAATCAGAACACCGGCACCTCAATCCACAACTGCCGGATCACGGCAGCCGATGACCTCGCAAACAATAGCGGCGCCAGAGTGAACACGTTCCTCGGGCGGCCATGGAAGGAGTACTCGAGGACGGTTTATATGCAGAGCTTTATGGATGATTTGATTAATCCGGCGGGGTGGAGGGCTTGGGATGGAGATTTTGCTTTGAACACTTTGTATTATGCGGAATTTGGGAATTTTGGGCCGGGATCTAACACGACGGAGAGGGTTAATTGGGTGGGATTTCATTTGATTGATGATACTGATGCTGGGAATTTCACGGCGGGGAATTTTTTGATGGCGGACGATTGGGTGCCGCAGACCGGCGTTCCCTACACCAGCGGCCTCACGGAGTAG

mRNA sequence

ATGGCGGCTGCCCCACCCACCACCGCCGCATTCTACTCTCTCCTCCTCATCTCCCTCTACATCACCGCCGCTGGAATCTCCACCTCCGCCGCTCCCACCGGTTCGGTGTGCAGCTCGGCCCCAGACCCCTCCTACTGCAAATCGGCCCTCCCAAACCAAACCGGCAATGTGTACTCGTACGGACGGTCGTCCGTCAGAAAGTCGCTGTCGTCGTCGCAGAAGTTCCTCCGGCTAGTGGAGAAGTATCTCCGGTCCCGGTCGTCGCTGACGGTGGCGGCAGTTAGGGCGCTGGAGGACTGCCAGCTGCTGGCGGGACTGAACATAGATTACCTGACGACGTCGTTCCGGACGGTGAACTCGACGAGCTGGACGCTGCCGGAGATGAAGGCGGAGGACGTGCAGAGCCTGTTGAGTGCGATATTGACGAACCAACAGACATGTTTGGATGGGCTTAAGGCGACGGCGGGGTCTTGGTCTCTCAAAAATGGCCTTTCCCTCCCACTTGCCGCTGACACCAAGCTCTACTCTATGTCCTTAGCCTTTTTCACCAAAGGTTGGGTACCAAAAAAGAAGAAAAAACCGACATGGACTTCAGCCGGGAGACAGGGCGGGTTCAGCAACGGACGAATGTCACTCAAAATGTCCAGCAGAACCAAAGCGATTTACGAGACGGCAACTCGCCGGAATCTGCTGCAGACTGACGACGGTGGCGACGATCAGATTTCGGTTAGGGACATCGTCGTCGTCAGTCAAGACGGCAGCGGAAATTTCACAACCATTAATGAAGCGATTGCGGCGGCGCCGAACAACTCTGCTCCGACGGACGGCTATTTCTTGATCTTCGTGGATGCTGGAGTTTATGAAGAATACGTGACGATTGCGAAGAATAAGAGGTACTTGATGATGATCGGAGATGGGATTAATCAGACCGTCGTCACTGGTAATCGGAACGTCGTCGACGGCTGGACCACTTTTAATTCCGCCACATTTGCGGTGGTCGGACCCGGATTCGTAGCAGTAAACATGACATTCAGAAACACCGCCGGAGCAATAAAACACCAAGCCGTGGCCGTCCGGAACAGCGCCGACTTATCAACATTCTACCTCTGCAGCTTCGAAGGCTACCAAGACACCTTATACACCCACTCCCTCCGCCAATTCTACCGTGACTGCGATATTTACGGCACCGTCGACTTCATCTTCGGCAACGCCGCCGTCGTCTTACAGAACTGCAACATCTACCCGCGTCTGCCGATGGCCAACCAGTTCAACGCCATCACTGCCCAAGGCCGGACCGACCCAAATCAGAACACCGGCACCTCAATCCACAACTGCCGGATCACGGCAGCCGATGACCTCGCAAACAATAGCGGCGCCAGAGTGAACACGTTCCTCGGGCGGCCATGGAAGGAGTACTCGAGGACGGTTTATATGCAGAGCTTTATGGATGATTTGATTAATCCGGCGGGGTGGAGGGCTTGGGATGGAGATTTTGCTTTGAACACTTTGTATTATGCGGAATTTGGGAATTTTGGGCCGGGATCTAACACGACGGAGAGGGTTAATTGGGTGGGATTTCATTTGATTGATGATACTGATGCTGGGAATTTCACGGCGGGGAATTTTTTGATGGCGGACGATTGGGTGCCGCAGACCGGCGTTCCCTACACCAGCGGCCTCACGGAGTAG

Coding sequence (CDS)

ATGGCGGCTGCCCCACCCACCACCGCCGCATTCTACTCTCTCCTCCTCATCTCCCTCTACATCACCGCCGCTGGAATCTCCACCTCCGCCGCTCCCACCGGTTCGGTGTGCAGCTCGGCCCCAGACCCCTCCTACTGCAAATCGGCCCTCCCAAACCAAACCGGCAATGTGTACTCGTACGGACGGTCGTCCGTCAGAAAGTCGCTGTCGTCGTCGCAGAAGTTCCTCCGGCTAGTGGAGAAGTATCTCCGGTCCCGGTCGTCGCTGACGGTGGCGGCAGTTAGGGCGCTGGAGGACTGCCAGCTGCTGGCGGGACTGAACATAGATTACCTGACGACGTCGTTCCGGACGGTGAACTCGACGAGCTGGACGCTGCCGGAGATGAAGGCGGAGGACGTGCAGAGCCTGTTGAGTGCGATATTGACGAACCAACAGACATGTTTGGATGGGCTTAAGGCGACGGCGGGGTCTTGGTCTCTCAAAAATGGCCTTTCCCTCCCACTTGCCGCTGACACCAAGCTCTACTCTATGTCCTTAGCCTTTTTCACCAAAGGTTGGGTACCAAAAAAGAAGAAAAAACCGACATGGACTTCAGCCGGGAGACAGGGCGGGTTCAGCAACGGACGAATGTCACTCAAAATGTCCAGCAGAACCAAAGCGATTTACGAGACGGCAACTCGCCGGAATCTGCTGCAGACTGACGACGGTGGCGACGATCAGATTTCGGTTAGGGACATCGTCGTCGTCAGTCAAGACGGCAGCGGAAATTTCACAACCATTAATGAAGCGATTGCGGCGGCGCCGAACAACTCTGCTCCGACGGACGGCTATTTCTTGATCTTCGTGGATGCTGGAGTTTATGAAGAATACGTGACGATTGCGAAGAATAAGAGGTACTTGATGATGATCGGAGATGGGATTAATCAGACCGTCGTCACTGGTAATCGGAACGTCGTCGACGGCTGGACCACTTTTAATTCCGCCACATTTGCGGTGGTCGGACCCGGATTCGTAGCAGTAAACATGACATTCAGAAACACCGCCGGAGCAATAAAACACCAAGCCGTGGCCGTCCGGAACAGCGCCGACTTATCAACATTCTACCTCTGCAGCTTCGAAGGCTACCAAGACACCTTATACACCCACTCCCTCCGCCAATTCTACCGTGACTGCGATATTTACGGCACCGTCGACTTCATCTTCGGCAACGCCGCCGTCGTCTTACAGAACTGCAACATCTACCCGCGTCTGCCGATGGCCAACCAGTTCAACGCCATCACTGCCCAAGGCCGGACCGACCCAAATCAGAACACCGGCACCTCAATCCACAACTGCCGGATCACGGCAGCCGATGACCTCGCAAACAATAGCGGCGCCAGAGTGAACACGTTCCTCGGGCGGCCATGGAAGGAGTACTCGAGGACGGTTTATATGCAGAGCTTTATGGATGATTTGATTAATCCGGCGGGGTGGAGGGCTTGGGATGGAGATTTTGCTTTGAACACTTTGTATTATGCGGAATTTGGGAATTTTGGGCCGGGATCTAACACGACGGAGAGGGTTAATTGGGTGGGATTTCATTTGATTGATGATACTGATGCTGGGAATTTCACGGCGGGGAATTTTTTGATGGCGGACGATTGGGTGCCGCAGACCGGCGTTCCCTACACCAGCGGCCTCACGGAGTAG

Protein sequence

MAAAPPTTAAFYSLLLISLYITAAGISTSAAPTGSVCSSAPDPSYCKSALPNQTGNVYSYGRSSVRKSLSSSQKFLRLVEKYLRSRSSLTVAAVRALEDCQLLAGLNIDYLTTSFRTVNSTSWTLPEMKAEDVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLAFFTKGWVPKKKKKPTWTSAGRQGGFSNGRMSLKMSSRTKAIYETATRRNLLQTDDGGDDQISVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNKRYLMMIGDGINQTVVTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNSADLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCNIYPRLPMANQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNSGARVNTFLGRPWKEYSRTVYMQSFMDDLINPAGWRAWDGDFALNTLYYAEFGNFGPGSNTTERVNWVGFHLIDDTDAGNFTAGNFLMADDWVPQTGVPYTSGLTE
Homology
BLAST of Carg07155 vs. NCBI nr
Match: KAG7014346.1 (putative pectinesterase/pectinesterase inhibitor 41, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1118.6 bits (2892), Expect = 0.0e+00
Identity = 562/562 (100.00%), Postives = 562/562 (100.00%), Query Frame = 0

Query: 1   MAAAPPTTAAFYSLLLISLYITAAGISTSAAPTGSVCSSAPDPSYCKSALPNQTGNVYSY 60
           MAAAPPTTAAFYSLLLISLYITAAGISTSAAPTGSVCSSAPDPSYCKSALPNQTGNVYSY
Sbjct: 1   MAAAPPTTAAFYSLLLISLYITAAGISTSAAPTGSVCSSAPDPSYCKSALPNQTGNVYSY 60

Query: 61  GRSSVRKSLSSSQKFLRLVEKYLRSRSSLTVAAVRALEDCQLLAGLNIDYLTTSFRTVNS 120
           GRSSVRKSLSSSQKFLRLVEKYLRSRSSLTVAAVRALEDCQLLAGLNIDYLTTSFRTVNS
Sbjct: 61  GRSSVRKSLSSSQKFLRLVEKYLRSRSSLTVAAVRALEDCQLLAGLNIDYLTTSFRTVNS 120

Query: 121 TSWTLPEMKAEDVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLA 180
           TSWTLPEMKAEDVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLA
Sbjct: 121 TSWTLPEMKAEDVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLA 180

Query: 181 FFTKGWVPKKKKKPTWTSAGRQGGFSNGRMSLKMSSRTKAIYETATRRNLLQTDDGGDDQ 240
           FFTKGWVPKKKKKPTWTSAGRQGGFSNGRMSLKMSSRTKAIYETATRRNLLQTDDGGDDQ
Sbjct: 181 FFTKGWVPKKKKKPTWTSAGRQGGFSNGRMSLKMSSRTKAIYETATRRNLLQTDDGGDDQ 240

Query: 241 ISVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNKRYL 300
           ISVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNKRYL
Sbjct: 241 ISVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNKRYL 300

Query: 301 MMIGDGINQTVVTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRN 360
           MMIGDGINQTVVTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRN
Sbjct: 301 MMIGDGINQTVVTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRN 360

Query: 361 SADLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCNIYPRLPMA 420
           SADLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCNIYPRLPMA
Sbjct: 361 SADLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCNIYPRLPMA 420

Query: 421 NQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNSGARVNTFLGRPWKEYSRTVYMQS 480
           NQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNSGARVNTFLGRPWKEYSRTVYMQS
Sbjct: 421 NQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNSGARVNTFLGRPWKEYSRTVYMQS 480

Query: 481 FMDDLINPAGWRAWDGDFALNTLYYAEFGNFGPGSNTTERVNWVGFHLIDDTDAGNFTAG 540
           FMDDLINPAGWRAWDGDFALNTLYYAEFGNFGPGSNTTERVNWVGFHLIDDTDAGNFTAG
Sbjct: 481 FMDDLINPAGWRAWDGDFALNTLYYAEFGNFGPGSNTTERVNWVGFHLIDDTDAGNFTAG 540

Query: 541 NFLMADDWVPQTGVPYTSGLTE 563
           NFLMADDWVPQTGVPYTSGLTE
Sbjct: 541 NFLMADDWVPQTGVPYTSGLTE 562

BLAST of Carg07155 vs. NCBI nr
Match: KAG6575804.1 (putative pectinesterase/pectinesterase inhibitor 41, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1115.5 bits (2884), Expect = 0.0e+00
Identity = 560/562 (99.64%), Postives = 562/562 (100.00%), Query Frame = 0

Query: 1   MAAAPPTTAAFYSLLLISLYITAAGISTSAAPTGSVCSSAPDPSYCKSALPNQTGNVYSY 60
           MAAAPPTTAAFYSLLLISLYITAAGISTSAAPTGSVCSSAPDPSYCKSALPNQTGNVYSY
Sbjct: 1   MAAAPPTTAAFYSLLLISLYITAAGISTSAAPTGSVCSSAPDPSYCKSALPNQTGNVYSY 60

Query: 61  GRSSVRKSLSSSQKFLRLVEKYLRSRSSLTVAAVRALEDCQLLAGLNIDYLTTSFRTVNS 120
           GRSSVRKSLSSSQKFLRLVEKYLRSRSSLTVAAVRALEDCQLLAGLNIDYLTTSFRTVNS
Sbjct: 61  GRSSVRKSLSSSQKFLRLVEKYLRSRSSLTVAAVRALEDCQLLAGLNIDYLTTSFRTVNS 120

Query: 121 TSWTLPEMKAEDVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLA 180
           TSWTLPEMKAEDVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLA
Sbjct: 121 TSWTLPEMKAEDVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLA 180

Query: 181 FFTKGWVPKKKKKPTWTSAGRQGGFSNGRMSLKMSSRTKAIYETATRRNLLQTDDGGDDQ 240
           FFTKGWVPKKKKKPTWTSAGRQGGFSNGRMSLKMSSRTKAIYETATRRNLLQT+DGGDDQ
Sbjct: 181 FFTKGWVPKKKKKPTWTSAGRQGGFSNGRMSLKMSSRTKAIYETATRRNLLQTEDGGDDQ 240

Query: 241 ISVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNKRYL 300
           ISVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNKRYL
Sbjct: 241 ISVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNKRYL 300

Query: 301 MMIGDGINQTVVTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRN 360
           MMIGDGINQTVVTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRN
Sbjct: 301 MMIGDGINQTVVTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRN 360

Query: 361 SADLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCNIYPRLPMA 420
           SADLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCNIYPRLPMA
Sbjct: 361 SADLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCNIYPRLPMA 420

Query: 421 NQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNSGARVNTFLGRPWKEYSRTVYMQS 480
           NQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNSGARVNTFLGRPWKEYSRTVYMQS
Sbjct: 421 NQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNSGARVNTFLGRPWKEYSRTVYMQS 480

Query: 481 FMDDLINPAGWRAWDGDFALNTLYYAEFGNFGPGSNTTERVNWVGFHLIDDTDAGNFTAG 540
           FMDDLINPAGWRAWDGDFALNTLYYAEFGNFGPGSNT+ERVNWVGFHLIDDTDAGNFTAG
Sbjct: 481 FMDDLINPAGWRAWDGDFALNTLYYAEFGNFGPGSNTSERVNWVGFHLIDDTDAGNFTAG 540

Query: 541 NFLMADDWVPQTGVPYTSGLTE 563
           NFLMADDWVPQTGVPYTSGLTE
Sbjct: 541 NFLMADDWVPQTGVPYTSGLTE 562

BLAST of Carg07155 vs. NCBI nr
Match: XP_022953705.1 (probable pectinesterase/pectinesterase inhibitor 7 [Cucurbita moschata])

HSP 1 Score: 1112.4 bits (2876), Expect = 0.0e+00
Identity = 558/562 (99.29%), Postives = 561/562 (99.82%), Query Frame = 0

Query: 1   MAAAPPTTAAFYSLLLISLYITAAGISTSAAPTGSVCSSAPDPSYCKSALPNQTGNVYSY 60
           MAAAPPTTAAFYSLLLISLYITAAGISTSAAPTGSVCSSAPDPSYCKSALPNQTGNVYSY
Sbjct: 1   MAAAPPTTAAFYSLLLISLYITAAGISTSAAPTGSVCSSAPDPSYCKSALPNQTGNVYSY 60

Query: 61  GRSSVRKSLSSSQKFLRLVEKYLRSRSSLTVAAVRALEDCQLLAGLNIDYLTTSFRTVNS 120
           GRSSVRKSLSSSQKFLRLVEKYLRSRSSLTVAAVRALEDCQLLAGLNIDYLTTSFRTVNS
Sbjct: 61  GRSSVRKSLSSSQKFLRLVEKYLRSRSSLTVAAVRALEDCQLLAGLNIDYLTTSFRTVNS 120

Query: 121 TSWTLPEMKAEDVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLA 180
           TSWTLPEMKAEDVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLA
Sbjct: 121 TSWTLPEMKAEDVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLA 180

Query: 181 FFTKGWVPKKKKKPTWTSAGRQGGFSNGRMSLKMSSRTKAIYETATRRNLLQTDDGGDDQ 240
           FFTKGWVPKKKKKPTWTSAGRQGGF+NGRMSLKMSSRTKAIYETATRRNLLQT+DGGDDQ
Sbjct: 181 FFTKGWVPKKKKKPTWTSAGRQGGFNNGRMSLKMSSRTKAIYETATRRNLLQTEDGGDDQ 240

Query: 241 ISVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNKRYL 300
           ISVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNKRYL
Sbjct: 241 ISVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNKRYL 300

Query: 301 MMIGDGINQTVVTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRN 360
           MMIGDGINQTVVTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRN AGAIKHQAVAVRN
Sbjct: 301 MMIGDGINQTVVTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNAAGAIKHQAVAVRN 360

Query: 361 SADLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCNIYPRLPMA 420
           SADLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCNIYPRLPMA
Sbjct: 361 SADLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCNIYPRLPMA 420

Query: 421 NQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNSGARVNTFLGRPWKEYSRTVYMQS 480
           NQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNSGARVNTFLGRPWKEYSRTVYMQS
Sbjct: 421 NQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNSGARVNTFLGRPWKEYSRTVYMQS 480

Query: 481 FMDDLINPAGWRAWDGDFALNTLYYAEFGNFGPGSNTTERVNWVGFHLIDDTDAGNFTAG 540
           FMDDLINPAGWRAWDGDFALNTLYYAEFGNFGPGSNT+ERVNWVGFHLIDDTDAGNFTAG
Sbjct: 481 FMDDLINPAGWRAWDGDFALNTLYYAEFGNFGPGSNTSERVNWVGFHLIDDTDAGNFTAG 540

Query: 541 NFLMADDWVPQTGVPYTSGLTE 563
           NFLMADDWVPQTGVPYTSGLTE
Sbjct: 541 NFLMADDWVPQTGVPYTSGLTE 562

BLAST of Carg07155 vs. NCBI nr
Match: XP_023548976.1 (probable pectinesterase/pectinesterase inhibitor 7 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1112.1 bits (2875), Expect = 0.0e+00
Identity = 558/562 (99.29%), Postives = 561/562 (99.82%), Query Frame = 0

Query: 1   MAAAPPTTAAFYSLLLISLYITAAGISTSAAPTGSVCSSAPDPSYCKSALPNQTGNVYSY 60
           MAAAPPTTAAFYSLLLISLYITAAGISTSAAPTGS CSSAPDPSYCKSALPNQTGNVYSY
Sbjct: 1   MAAAPPTTAAFYSLLLISLYITAAGISTSAAPTGSECSSAPDPSYCKSALPNQTGNVYSY 60

Query: 61  GRSSVRKSLSSSQKFLRLVEKYLRSRSSLTVAAVRALEDCQLLAGLNIDYLTTSFRTVNS 120
           GRSSVRKSLSSSQKFLRLV+KYLRSRSSLTVAAVRALEDCQLLAGLNIDYLTTSFRTVNS
Sbjct: 61  GRSSVRKSLSSSQKFLRLVQKYLRSRSSLTVAAVRALEDCQLLAGLNIDYLTTSFRTVNS 120

Query: 121 TSWTLPEMKAEDVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLA 180
           TSWTLPEMKAEDVQSLLSAILTNQQTCLDGL+ATAGSWSLKNGLSLPLAADTKLYSMSLA
Sbjct: 121 TSWTLPEMKAEDVQSLLSAILTNQQTCLDGLQATAGSWSLKNGLSLPLAADTKLYSMSLA 180

Query: 181 FFTKGWVPKKKKKPTWTSAGRQGGFSNGRMSLKMSSRTKAIYETATRRNLLQTDDGGDDQ 240
           FFTKGWVPKKKKKPTWTSAGRQGGFSNGRMSLKMSSRTKAIYETATRRNLLQTDDGGDDQ
Sbjct: 181 FFTKGWVPKKKKKPTWTSAGRQGGFSNGRMSLKMSSRTKAIYETATRRNLLQTDDGGDDQ 240

Query: 241 ISVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNKRYL 300
           ISVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNKRYL
Sbjct: 241 ISVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNKRYL 300

Query: 301 MMIGDGINQTVVTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRN 360
           MMIGDGINQTVVTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRN
Sbjct: 301 MMIGDGINQTVVTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRN 360

Query: 361 SADLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCNIYPRLPMA 420
           SADLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCNIYPRLPMA
Sbjct: 361 SADLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCNIYPRLPMA 420

Query: 421 NQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNSGARVNTFLGRPWKEYSRTVYMQS 480
           NQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNSGARVNTFLGRPWKEYSRTVYMQS
Sbjct: 421 NQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNSGARVNTFLGRPWKEYSRTVYMQS 480

Query: 481 FMDDLINPAGWRAWDGDFALNTLYYAEFGNFGPGSNTTERVNWVGFHLIDDTDAGNFTAG 540
           FMDDLINPAGWRAWDGDFALNTLYYAEFGNFGPGSNT+ERVNWVGFHLIDDTDAGNFTAG
Sbjct: 481 FMDDLINPAGWRAWDGDFALNTLYYAEFGNFGPGSNTSERVNWVGFHLIDDTDAGNFTAG 540

Query: 541 NFLMADDWVPQTGVPYTSGLTE 563
           NFLMADDWVPQTGVPYTSGLTE
Sbjct: 541 NFLMADDWVPQTGVPYTSGLTE 562

BLAST of Carg07155 vs. NCBI nr
Match: XP_022991223.1 (probable pectinesterase/pectinesterase inhibitor 7 [Cucurbita maxima])

HSP 1 Score: 1097.0 bits (2836), Expect = 0.0e+00
Identity = 551/562 (98.04%), Postives = 555/562 (98.75%), Query Frame = 0

Query: 1   MAAAPPTTAAFYSLLLISLYITAAGISTSAAPTGSVCSSAPDPSYCKSALPNQTGNVYSY 60
           MAAAPPTT AFY LLLISLYITAAGISTSAAPTGSVCSSAPDPSYCKSALPNQTGNVYSY
Sbjct: 1   MAAAPPTTPAFYYLLLISLYITAAGISTSAAPTGSVCSSAPDPSYCKSALPNQTGNVYSY 60

Query: 61  GRSSVRKSLSSSQKFLRLVEKYLRSRSSLTVAAVRALEDCQLLAGLNIDYLTTSFRTVNS 120
           GRSSVRKSLSSSQKFLRLVEKYLRSRSSLTVAAVR+LEDCQLLAGLNIDYLTTSFRTVNS
Sbjct: 61  GRSSVRKSLSSSQKFLRLVEKYLRSRSSLTVAAVRSLEDCQLLAGLNIDYLTTSFRTVNS 120

Query: 121 TSWTLPEMKAEDVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLA 180
           TSWTLPEMKAEDVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPL ADTKLYSMSLA
Sbjct: 121 TSWTLPEMKAEDVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLDADTKLYSMSLA 180

Query: 181 FFTKGWVPKKKKKPTWTSAGRQGGFSNGRMSLKMSSRTKAIYETATRRNLLQTDDGGDDQ 240
           FFTKGWVPKKKKK TWTSAGRQGGFSNGRMS KMSSRTKAIYE ATRRNLLQTDDGGDDQ
Sbjct: 181 FFTKGWVPKKKKKQTWTSAGRQGGFSNGRMSFKMSSRTKAIYEMATRRNLLQTDDGGDDQ 240

Query: 241 ISVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNKRYL 300
           ISVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNKRYL
Sbjct: 241 ISVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNKRYL 300

Query: 301 MMIGDGINQTVVTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRN 360
           MMIGDGINQTVVTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRN
Sbjct: 301 MMIGDGINQTVVTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRN 360

Query: 361 SADLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCNIYPRLPMA 420
           SADLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCNIYPRLPMA
Sbjct: 361 SADLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCNIYPRLPMA 420

Query: 421 NQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNSGARVNTFLGRPWKEYSRTVYMQS 480
           NQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANN+GARVNTFLGRPWKEYSRTVYMQS
Sbjct: 421 NQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNTGARVNTFLGRPWKEYSRTVYMQS 480

Query: 481 FMDDLINPAGWRAWDGDFALNTLYYAEFGNFGPGSNTTERVNWVGFHLIDDTDAGNFTAG 540
           FM+DLINPAGWRAWDGDFALNT YYAEFGNFGPGSNT+ERVNWVGFHLIDDTDAGNFTAG
Sbjct: 481 FMNDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWVGFHLIDDTDAGNFTAG 540

Query: 541 NFLMADDWVPQTGVPYTSGLTE 563
           NFLMADDWVPQTGVPYTSGLTE
Sbjct: 541 NFLMADDWVPQTGVPYTSGLTE 562

BLAST of Carg07155 vs. ExPASy Swiss-Prot
Match: Q9SRX4 (Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PME7 PE=2 SV=1)

HSP 1 Score: 685.3 bits (1767), Expect = 6.0e-196
Identity = 345/573 (60.21%), Postives = 426/573 (74.35%), Query Frame = 0

Query: 12  YSLLLISLYI-TAAGISTSAAPTGSVCSSAPDPSYCKSALPNQTGNVYSYGRSSVRKSLS 71
           + L+ +S ++ +    S + + + ++C + PDP YCKS  P+  GNV  YG  S+RKSLS
Sbjct: 6   FILITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFPHSQGNVQQYGCFSIRKSLS 65

Query: 72  SSQKFLRLVEKYLRSRSSLT-VAAVRALEDCQLLAGLNIDYLTTSFRTVNSTS-----WT 131
            S+KF+R V++Y++  + L+  A +RAL+DC+ LAGL +DYL TSF TVN TS       
Sbjct: 66  QSRKFIRTVDRYIKRNAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAKTSFKP 125

Query: 132 LPEMKAEDVQSLLSAILTNQQTCLDGLKATA---GSWSLKNGLSLPLAADTKLYSMSLAF 191
           L   KA+D+Q+LLSA LTN+QTCL+GL   A    +W+++ G++LPL  DTKL  +SLA 
Sbjct: 126 LSFPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKLLGVSLAL 185

Query: 192 FTKGWVPKKKKKPTWTSAGRQGG----------FSNGRMSLKMSSRTKAIYETATRRNLL 251
           FTKGWVPKKKK+  +  A  + G          F NG + LKM+ +TKA+YE+ +RR L 
Sbjct: 186 FTKGWVPKKKKRAGFAWAQPRSGSSTHTKPFRLFRNGALPLKMTEKTKAVYESLSRRKLA 245

Query: 252 QTDDGGD----DQISVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVY 311
             D  GD      + + DIV VSQDG+GNFT I  A+AAAPNN+  + G+FLI+V AG+Y
Sbjct: 246 DGDSNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIY 305

Query: 312 EEYVTIAKNKRYLMMIGDGINQTVVTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNT 371
           EEY++IAKNKRY+MMIGDGINQTVVTGNR+VVDGWTTFNSATFAV  P FVAVN+TFRNT
Sbjct: 306 EEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRNT 365

Query: 372 AGAIKHQAVAVRNSADLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVV 431
           AG  KHQAVA+R+ AD S FY CSFE YQDTLYTHSLRQFYR+CD+YGTVDFIFGNAAVV
Sbjct: 366 AGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVV 425

Query: 432 LQNCNIYPRLPMANQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNSGARVNTFLGR 491
            QNCN+YPR PM NQFNAITAQGR+DPNQNTGTSI NC I  ADDL  +S   V T+LGR
Sbjct: 426 FQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLV-SSNYTVKTYLGR 485

Query: 492 PWKEYSRTVYMQSFMDDLINPAGWRAWDGDFALNTLYYAEFGNFGPGSNTTERVNWVGFH 551
           PWKEYSRTVYMQS++D  + P GWR W+GDFAL+TLYYAE+ N GPGSNTT RV W G+H
Sbjct: 486 PWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYH 545

Query: 552 LIDDTDAGNFTAGNFLMADDWVPQTGVPYTSGL 561
           +I+ TDA NFT     +  DW+ +TGVPYTSGL
Sbjct: 546 VINSTDAANFTVTGLFIEADWIWKTGVPYTSGL 577

BLAST of Carg07155 vs. ExPASy Swiss-Prot
Match: Q8RXK7 (Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3702 GN=PME41 PE=2 SV=2)

HSP 1 Score: 675.6 bits (1742), Expect = 4.7e-193
Identity = 350/568 (61.62%), Postives = 432/568 (76.06%), Query Frame = 0

Query: 14  LLLISLYITAAGI---STSAAPTGS---VCSSAPDPSYCKSALPNQT-GNVYSYGRSSVR 73
           L L++L+++   +   S +  P+ S   +C + PDP +CKS  P  + G+V  YGR S+R
Sbjct: 6   LFLVTLFLSLQTLFIASQTLLPSNSSSTICKTTPDPKFCKSVFPQTSQGDVREYGRFSLR 65

Query: 74  KSLSSSQKFLRLVEKYL-RSRSSLTVAAVRALEDCQLLAGLNIDYLTTSFRTVNST--SW 133
           KSL+ S+KF R +++YL R+ + L+ +AV AL+DC+ LA L  DYL TSF TVN T  S 
Sbjct: 66  KSLTQSRKFTRTIDRYLKRNNALLSQSAVGALQDCRYLASLTTDYLITSFETVNITTSSK 125

Query: 134 TLPEMKAEDVQSLLSAILTNQQTCLDGLK-ATAGSWSLKNGLSLPLAADTKLYSMSLAFF 193
           TL   KA+++Q+LLSA LTN+QTCLDG+  A + SW+++NG++LPL  DTKL+S+SLA F
Sbjct: 126 TLSFSKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLFSVSLALF 185

Query: 194 TKGWVPKKKK----------KPTWTSAGRQGGFSNGRMSLKMSSRTKAIYETATRRNLLQ 253
           TKGWVPKKKK          K T +       F NG + LKM+  T+A+YE+ +RR L  
Sbjct: 186 TKGWVPKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESLSRRKLAD 245

Query: 254 TDDGGDDQISVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVT 313
            DD   + + V DIV V+Q+G+GNFTTI EA+ +APN +  T GYF+I+V +GVYEE V 
Sbjct: 246 -DDNDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENVV 305

Query: 314 IAKNKRYLMMIGDGINQTVVTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIK 373
           IAKNKRYLMMIGDGIN+TVVTGNRNVVDGWTTFNSATFAV  P FVAVNMTFRNTAG  K
Sbjct: 306 IAKNKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTAGPEK 365

Query: 374 HQAVAVRNSADLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCN 433
           HQAVA+R+SADLS FY CSFE YQDTLYTHSLRQFYR+CDIYGTVDFIFGNAAVV Q+CN
Sbjct: 366 HQAVAMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDCN 425

Query: 434 IYPRLPMANQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNSGARVNTFLGRPWKEY 493
           +YPR PM NQFNAITAQGRTDPNQNTG SIHNC I  ADDL  +S   V T+LGRPWKEY
Sbjct: 426 LYPRQPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLV-SSNYTVKTYLGRPWKEY 485

Query: 494 SRTVYMQSFMDDLINPAGWRAWDGDFALNTLYYAEFGNFGPGSNTTERVNWVGFHLIDDT 553
           SRTV+MQS++D+++ P GWR W+GDFAL+TLYYAE+ N G GS+TT+RV W G+H+I+ T
Sbjct: 486 SRTVFMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVINST 545

Query: 554 DAGNFTAGNFLMADDWVPQTGVPYTSGL 561
           DA NFT  NFL+ D W+ Q+GVPY SGL
Sbjct: 546 DANNFTVENFLLGDGWMVQSGVPYISGL 571

BLAST of Carg07155 vs. ExPASy Swiss-Prot
Match: O22256 (Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3702 GN=PME20 PE=2 SV=2)

HSP 1 Score: 656.4 bits (1692), Expect = 3.0e-187
Identity = 332/549 (60.47%), Postives = 414/549 (75.41%), Query Frame = 0

Query: 17  ISLYITAAGISTSAAPTGSVCSSAPDPSYCKSALPNQTGNVYSYGRSSVRKSLSSSQKFL 76
           IS  I A G +TS +   ++C  APDPSYC+S LPNQ G++YSYGR S+R+SLS +++F+
Sbjct: 22  ISAQIPAIGNATSPS---NICRFAPDPSYCRSVLPNQPGDIYSYGRLSLRRSLSRARRFI 81

Query: 77  RLVEKYLRSRSSLTV-AAVRALEDCQLLAGLNIDYLTTSFRTVNSTSWTLPEMKAEDVQS 136
            +++  L  +  +   + V ALEDC+ LA L +DYL +S +T +ST  TL   +AEDV +
Sbjct: 82  SMIDAELDRKGKVAAKSTVGALEDCKFLASLTMDYLLSSSQTADSTK-TLSLSRAEDVHT 141

Query: 137 LLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLAFFTKGWVPKKKK-KP 196
            LSA +TN+QTCL+GLK+TA     +NGLS  L  DTKLY +SLA F+KGWVP++++ +P
Sbjct: 142 FLSAAITNEQTCLEGLKSTAS----ENGLSGDLFNDTKLYGVSLALFSKGWVPRRQRSRP 201

Query: 197 TWTSAGRQG---GFSNGRMSLKMSSRTKAIYETATRRNLLQTDDGGDDQISVRDIVVVSQ 256
            W    R     GF NG++ LKM+ R +A+Y T TRR LLQ+D    D + V DIV V Q
Sbjct: 202 IWQPQARFKKFFGFRNGKLPLKMTERARAVYNTVTRRKLLQSD---ADAVQVSDIVTVIQ 261

Query: 257 DGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNKRYLMMIGDGINQTV 316
           +G+GNFTTIN AIAAAPN +  ++GYFLI+V AG+YEEYV + KNKRY+MMIGDGINQTV
Sbjct: 262 NGTGNFTTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDGINQTV 321

Query: 317 VTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNSADLSTFYLCS 376
           +TGNR+VVDGWTTFNSATF + GP F+ VN+T RNTAG  K QAVA+R+  DLS FY CS
Sbjct: 322 ITGNRSVVDGWTTFNSATFILSGPNFIGVNITIRNTAGPTKGQAVALRSGGDLSVFYSCS 381

Query: 377 FEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCNIYPRLPMANQFNAITAQGR 436
           FE YQDTLYTHSLRQFYR+CD+YGTVDFIFGNAAVVLQNCN+YPR P   Q N +TAQGR
Sbjct: 382 FEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQSNEVTAQGR 441

Query: 437 TDPNQNTGTSIHNCRITAADDLANNSGARVNTFLGRPWKEYSRTVYMQSFMDDLINPAGW 496
           TDPNQNTGT+IH C I  ADDLA  S   V T+LGRPWKEYSRTV MQ+++D  + P+GW
Sbjct: 442 TDPNQNTGTAIHGCTIRPADDLA-TSNYTVKTYLGRPWKEYSRTVVMQTYIDGFLEPSGW 501

Query: 497 RAWDGDFALNTLYYAEFGNFGPGSNTTERVNWVGFHLIDDTDAGNFTAGNFLMADDWVPQ 556
            AW GDFAL+TLYYAE+ N GPGS+TT RV W G+H+I+ TDA NFT  NFL+ + W+ Q
Sbjct: 502 NAWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYHVINATDASNFTVTNFLVGEGWIGQ 558

Query: 557 TGVPYTSGL 561
           TGVP+  GL
Sbjct: 562 TGVPFVGGL 558

BLAST of Carg07155 vs. ExPASy Swiss-Prot
Match: Q94CB1 (Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3702 GN=PME25 PE=2 SV=1)

HSP 1 Score: 491.1 bits (1263), Expect = 1.7e-137
Identity = 263/549 (47.91%), Postives = 352/549 (64.12%), Query Frame = 0

Query: 27  STSAAPTGSVCSSAPDPSYCK---SALPNQTGNVYSYGRSSVRKSLSSSQKFLRLVEKYL 86
           S S +P+   C S P P  C+   +A+ +   + Y YG+ ++++ L  + +  +++  Y 
Sbjct: 74  SQSQSPS-LACKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVITSYA 133

Query: 87  R----SRSSLTVAAVRALEDCQLLAGLNIDYLTTSFRTVNSTSWTLPEMKAEDVQSLLSA 146
           R       S T   + A+ DC  L+ L+++YL T   T   T+  +     E V SLLS 
Sbjct: 134 RRVESKPGSATAEEIGAVADCGELSELSVNYLET-VTTELKTAQVMTAALVEHVNSLLSG 193

Query: 147 ILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLAFFTKGWVPKKKKKPTWTSA 206
           ++TNQQTCLDGL      ++    +  P+   T+LYS+SL   +      +  K    S 
Sbjct: 194 VVTNQQTCLDGLVEAKSGFAA--AIGSPMGNLTRLYSISLGLVSHAL--NRNLKRFKASK 253

Query: 207 GRQGGFSNGRMSLKMSSRTKAIYETA--------TRRNLLQTDDGGDDQISVRDIVVVSQ 266
           G+  G  N      + +  K + +T         T RNL +  +     I V   V+V  
Sbjct: 254 GKILGGGNSTYREPLETLIKGLRKTCDNDKDCRKTSRNLGELGETSGGSILVSKAVIVGP 313

Query: 267 DGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNKRYLMMIGDGINQTV 326
             S NFTTI +AIAAAPNN+ P DGYF+I+   GVYEEY+ +  NK+ LM++GDGIN+T+
Sbjct: 314 FKSDNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINKTI 373

Query: 327 VTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNSADLSTFYLCS 386
           +TGN NVVDGWTT+N ++FAVVG  F+AV++TFRNTAG  KHQAVA+RN+A+ S+FY CS
Sbjct: 374 ITGNHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYRCS 433

Query: 387 FEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCNIYPRLPMANQFNAITAQGR 446
           FEGYQDTLY HSLRQFYR+CDIYGTVDFIFGNAA + QNCNIY R PMA Q NAITA GR
Sbjct: 434 FEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAHGR 493

Query: 447 TDPNQNTGTSIHNCRITAADDLANNSGARVNTFLGRPWKEYSRTVYMQSFMDDLINPAGW 506
            DPNQNTG SI NC I AA DLA    + + TFLGRPWK YSRTV+MQS++ D++ P GW
Sbjct: 494 LDPNQNTGISIINCTIKAAPDLAAEPKSAM-TFLGRPWKPYSRTVFMQSYISDIVQPVGW 553

Query: 507 RAWDGDFALNTLYYAEFGNFGPGSNTTERVNWVGFHLIDDTDAGNFTAGNFLMADDWVPQ 561
             W+G   L+T+YY E+ NFGPG+NT +RV W+G++L++  +A NFT  NF M D W+PQ
Sbjct: 554 LEWNGTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTMGDTWLPQ 613

BLAST of Carg07155 vs. ExPASy Swiss-Prot
Match: Q9FF77 (Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana OX=3702 GN=PME47 PE=2 SV=1)

HSP 1 Score: 479.9 bits (1234), Expect = 3.8e-134
Identity = 256/547 (46.80%), Postives = 350/547 (63.99%), Query Frame = 0

Query: 29  SAAPTGSVCSSAPDPSYCK---SALPNQTGNVYSYGRSSVRKSLSSSQKFLRLVEKYLR- 88
           S  P    C S P P  C+   SA+ +   + Y YG+ ++++ L  +++  +++ ++ + 
Sbjct: 80  SPLPPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVINRFAQR 139

Query: 89  ---SRSSLTVAAVRALEDCQLLAGLNIDYLTTSFRTVNSTSWTLPEMKAEDVQSLLSAIL 148
                 + TV  V A+ DC  LA L+++YL T    + +    +     + V SLL  ++
Sbjct: 140 VEADPGTSTVEEVSAVADCGELAELSVEYLETVTEELKAAE-LMTAALVDRVTSLLGGVV 199

Query: 149 TNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLAFFTKGWVPKKKKKPTWTSAGR 208
           TNQQTCLDGL      ++    +  PL   T+LYS+SL   +      +  K    S G+
Sbjct: 200 TNQQTCLDGLVDAKSGFA--TAIGTPLGNLTRLYSVSLGLVSHAL--NRNLKRYKGSKGK 259

Query: 209 QGGFSNGRMSLKMSSRTKAIYETATR--------RNLLQTDDGGDDQISVRDIVVVSQDG 268
             G  N  +   + +  K + +T  +        RNL +  +     I VR+ V V    
Sbjct: 260 IFGGGNKPVREPLETLIKVLRKTCDKGKDCRKANRNLGELGETSGGSILVREAVTVGPYE 319

Query: 269 SGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNKRYLMMIGDGINQTVVT 328
           + NF TI EA+AAAPN++ P  GYF+I+  AG+YEEYV I+  KR +M+IGDGIN+T+++
Sbjct: 320 TDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINKTIIS 379

Query: 329 GNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNSADLSTFYLCSFE 388
           GN + +DGWTT+NS+TFAVVG  FVAV++TFRNTAG  KHQAVAVRN+AD STFY CSFE
Sbjct: 380 GNHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYRCSFE 439

Query: 389 GYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCNIYPRLPMANQFNAITAQGRTD 448
           GYQDTLY HSLRQFYR+CDIYGT+DFIFGNAA + QNCNIY R PMANQ NA+TA GRTD
Sbjct: 440 GYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAHGRTD 499

Query: 449 PNQNTGTSIHNCRITAADDLANNSGARVNTFLGRPWKEYSRTVYMQSFMDDLINPAGWRA 508
           PNQ TG SI NC I AA DLA +  + + TFLGRPWK YSRTVY+QS++ D++ P GW  
Sbjct: 500 PNQKTGISIINCTIGAAPDLAADPKSTM-TFLGRPWKPYSRTVYIQSYISDVVQPVGWLE 559

Query: 509 WDGDFALNTLYYAEFGNFGPGSNTTERVNWVGFHLIDDTDAGNFTAGNFLMADDWVPQTG 561
           W+G   L+T+ Y E+ NFGPG++T++RV W G+ L++   A NFT  NF + D W+PQT 
Sbjct: 560 WNGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLLNLVQAMNFTVYNFTLGDTWLPQTD 619

BLAST of Carg07155 vs. ExPASy TrEMBL
Match: A0A6J1GQE6 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111456155 PE=3 SV=1)

HSP 1 Score: 1112.4 bits (2876), Expect = 0.0e+00
Identity = 558/562 (99.29%), Postives = 561/562 (99.82%), Query Frame = 0

Query: 1   MAAAPPTTAAFYSLLLISLYITAAGISTSAAPTGSVCSSAPDPSYCKSALPNQTGNVYSY 60
           MAAAPPTTAAFYSLLLISLYITAAGISTSAAPTGSVCSSAPDPSYCKSALPNQTGNVYSY
Sbjct: 1   MAAAPPTTAAFYSLLLISLYITAAGISTSAAPTGSVCSSAPDPSYCKSALPNQTGNVYSY 60

Query: 61  GRSSVRKSLSSSQKFLRLVEKYLRSRSSLTVAAVRALEDCQLLAGLNIDYLTTSFRTVNS 120
           GRSSVRKSLSSSQKFLRLVEKYLRSRSSLTVAAVRALEDCQLLAGLNIDYLTTSFRTVNS
Sbjct: 61  GRSSVRKSLSSSQKFLRLVEKYLRSRSSLTVAAVRALEDCQLLAGLNIDYLTTSFRTVNS 120

Query: 121 TSWTLPEMKAEDVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLA 180
           TSWTLPEMKAEDVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLA
Sbjct: 121 TSWTLPEMKAEDVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLA 180

Query: 181 FFTKGWVPKKKKKPTWTSAGRQGGFSNGRMSLKMSSRTKAIYETATRRNLLQTDDGGDDQ 240
           FFTKGWVPKKKKKPTWTSAGRQGGF+NGRMSLKMSSRTKAIYETATRRNLLQT+DGGDDQ
Sbjct: 181 FFTKGWVPKKKKKPTWTSAGRQGGFNNGRMSLKMSSRTKAIYETATRRNLLQTEDGGDDQ 240

Query: 241 ISVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNKRYL 300
           ISVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNKRYL
Sbjct: 241 ISVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNKRYL 300

Query: 301 MMIGDGINQTVVTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRN 360
           MMIGDGINQTVVTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRN AGAIKHQAVAVRN
Sbjct: 301 MMIGDGINQTVVTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNAAGAIKHQAVAVRN 360

Query: 361 SADLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCNIYPRLPMA 420
           SADLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCNIYPRLPMA
Sbjct: 361 SADLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCNIYPRLPMA 420

Query: 421 NQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNSGARVNTFLGRPWKEYSRTVYMQS 480
           NQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNSGARVNTFLGRPWKEYSRTVYMQS
Sbjct: 421 NQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNSGARVNTFLGRPWKEYSRTVYMQS 480

Query: 481 FMDDLINPAGWRAWDGDFALNTLYYAEFGNFGPGSNTTERVNWVGFHLIDDTDAGNFTAG 540
           FMDDLINPAGWRAWDGDFALNTLYYAEFGNFGPGSNT+ERVNWVGFHLIDDTDAGNFTAG
Sbjct: 481 FMDDLINPAGWRAWDGDFALNTLYYAEFGNFGPGSNTSERVNWVGFHLIDDTDAGNFTAG 540

Query: 541 NFLMADDWVPQTGVPYTSGLTE 563
           NFLMADDWVPQTGVPYTSGLTE
Sbjct: 541 NFLMADDWVPQTGVPYTSGLTE 562

BLAST of Carg07155 vs. ExPASy TrEMBL
Match: A0A6J1JVJ5 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111487941 PE=3 SV=1)

HSP 1 Score: 1097.0 bits (2836), Expect = 0.0e+00
Identity = 551/562 (98.04%), Postives = 555/562 (98.75%), Query Frame = 0

Query: 1   MAAAPPTTAAFYSLLLISLYITAAGISTSAAPTGSVCSSAPDPSYCKSALPNQTGNVYSY 60
           MAAAPPTT AFY LLLISLYITAAGISTSAAPTGSVCSSAPDPSYCKSALPNQTGNVYSY
Sbjct: 1   MAAAPPTTPAFYYLLLISLYITAAGISTSAAPTGSVCSSAPDPSYCKSALPNQTGNVYSY 60

Query: 61  GRSSVRKSLSSSQKFLRLVEKYLRSRSSLTVAAVRALEDCQLLAGLNIDYLTTSFRTVNS 120
           GRSSVRKSLSSSQKFLRLVEKYLRSRSSLTVAAVR+LEDCQLLAGLNIDYLTTSFRTVNS
Sbjct: 61  GRSSVRKSLSSSQKFLRLVEKYLRSRSSLTVAAVRSLEDCQLLAGLNIDYLTTSFRTVNS 120

Query: 121 TSWTLPEMKAEDVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLA 180
           TSWTLPEMKAEDVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPL ADTKLYSMSLA
Sbjct: 121 TSWTLPEMKAEDVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLDADTKLYSMSLA 180

Query: 181 FFTKGWVPKKKKKPTWTSAGRQGGFSNGRMSLKMSSRTKAIYETATRRNLLQTDDGGDDQ 240
           FFTKGWVPKKKKK TWTSAGRQGGFSNGRMS KMSSRTKAIYE ATRRNLLQTDDGGDDQ
Sbjct: 181 FFTKGWVPKKKKKQTWTSAGRQGGFSNGRMSFKMSSRTKAIYEMATRRNLLQTDDGGDDQ 240

Query: 241 ISVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNKRYL 300
           ISVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNKRYL
Sbjct: 241 ISVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNKRYL 300

Query: 301 MMIGDGINQTVVTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRN 360
           MMIGDGINQTVVTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRN
Sbjct: 301 MMIGDGINQTVVTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRN 360

Query: 361 SADLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCNIYPRLPMA 420
           SADLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCNIYPRLPMA
Sbjct: 361 SADLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCNIYPRLPMA 420

Query: 421 NQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNSGARVNTFLGRPWKEYSRTVYMQS 480
           NQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANN+GARVNTFLGRPWKEYSRTVYMQS
Sbjct: 421 NQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNTGARVNTFLGRPWKEYSRTVYMQS 480

Query: 481 FMDDLINPAGWRAWDGDFALNTLYYAEFGNFGPGSNTTERVNWVGFHLIDDTDAGNFTAG 540
           FM+DLINPAGWRAWDGDFALNT YYAEFGNFGPGSNT+ERVNWVGFHLIDDTDAGNFTAG
Sbjct: 481 FMNDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWVGFHLIDDTDAGNFTAG 540

Query: 541 NFLMADDWVPQTGVPYTSGLTE 563
           NFLMADDWVPQTGVPYTSGLTE
Sbjct: 541 NFLMADDWVPQTGVPYTSGLTE 562

BLAST of Carg07155 vs. ExPASy TrEMBL
Match: A0A5D3D3H4 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G001320 PE=3 SV=1)

HSP 1 Score: 1010.4 bits (2611), Expect = 3.0e-291
Identity = 508/562 (90.39%), Postives = 532/562 (94.66%), Query Frame = 0

Query: 3   AAPPTTAAFYSLLLISLYITAAGISTSAAPTGSVCSSAPDPSYCKSALPNQTGNVYSYGR 62
           AA  T AAFY L+LISLYIT     TSA+PTGSVCSS PDPSYCKSALPNQTGNVYSYGR
Sbjct: 2   AAGTTAAAFYVLILISLYIT-----TSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGR 61

Query: 63  SSVRKSLSSSQKFLRLVEKYLRSRSSLTVAAVRALEDCQLLAGLNIDYLTTSFRTVNSTS 122
           SS RKSLSSSQKFLRLVEK+LRSRSSLTVAAVRALEDCQLLAGLNIDYL TSF+ VN+TS
Sbjct: 62  SSFRKSLSSSQKFLRLVEKHLRSRSSLTVAAVRALEDCQLLAGLNIDYLRTSFQAVNATS 121

Query: 123 WTLPEMKAEDVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLAFF 182
             L EMKA+DVQSLLSAILTNQQTCLDG+KATAGSWSLKNGLS PLA+DTKLYS+SLAFF
Sbjct: 122 RALTEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFF 181

Query: 183 TKGWVPKKKKKPTWTSAGRQGGFSNGRMSLKMSSRTKAIYETATRRNLLQTDD--GGDDQ 242
           TKGWVPKKKKKPTW +AGRQGGF NGRMSLKMSSRT+AIYETATRRNLLQTDD  G DDQ
Sbjct: 182 TKGWVPKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTQAIYETATRRNLLQTDDDGGDDDQ 241

Query: 243 ISVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNKRYL 302
           I+VRDIVVVSQDG+GNFTTINEAIAAAPNNSAPTDGYFLIFV AGVYEEYV+IAKNKRYL
Sbjct: 242 IAVRDIVVVSQDGTGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVSIAKNKRYL 301

Query: 303 MMIGDGINQTVVTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRN 362
           MMIGDGINQT+VTGNR+VVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVA+RN
Sbjct: 302 MMIGDGINQTIVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVALRN 361

Query: 363 SADLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCNIYPRLPMA 422
            ADLSTFYLCSFE YQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVV QNCNIYPRLPM+
Sbjct: 362 GADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMS 421

Query: 423 NQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNSGARVNTFLGRPWKEYSRTVYMQS 482
           NQFNAITAQGRTDPNQNTGTSI+NCRITAADDLANNSGA V TFLGRPWKEYSRTVYMQS
Sbjct: 422 NQFNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQS 481

Query: 483 FMDDLINPAGWRAWDGDFALNTLYYAEFGNFGPGSNTTERVNWVGFHLIDDTDAGNFTAG 542
           FMDDLINPAGWRAWDGDFALNT YYAEFGNFGPGSNT+ERVNW GFHLI+DTDAGNFTAG
Sbjct: 482 FMDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAG 541

Query: 543 NFLMADDWVPQTGVPYTSGLTE 563
           NF++ADDW+PQTGVPYTSGLTE
Sbjct: 542 NFVLADDWLPQTGVPYTSGLTE 558

BLAST of Carg07155 vs. ExPASy TrEMBL
Match: A0A1S3BSI2 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492791 PE=3 SV=1)

HSP 1 Score: 1010.4 bits (2611), Expect = 3.0e-291
Identity = 508/562 (90.39%), Postives = 532/562 (94.66%), Query Frame = 0

Query: 3   AAPPTTAAFYSLLLISLYITAAGISTSAAPTGSVCSSAPDPSYCKSALPNQTGNVYSYGR 62
           AA  T AAFY L+LISLYIT     TSA+PTGSVCSS PDPSYCKSALPNQTGNVYSYGR
Sbjct: 2   AAGTTAAAFYVLILISLYIT-----TSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGR 61

Query: 63  SSVRKSLSSSQKFLRLVEKYLRSRSSLTVAAVRALEDCQLLAGLNIDYLTTSFRTVNSTS 122
           SS RKSLSSSQKFLRLVEK+LRSRSSLTVAAVRALEDCQLLAGLNIDYL TSF+ VN+TS
Sbjct: 62  SSFRKSLSSSQKFLRLVEKHLRSRSSLTVAAVRALEDCQLLAGLNIDYLRTSFQAVNATS 121

Query: 123 WTLPEMKAEDVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLAFF 182
             L EMKA+DVQSLLSAILTNQQTCLDG+KATAGSWSLKNGLS PLA+DTKLYS+SLAFF
Sbjct: 122 RALTEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFF 181

Query: 183 TKGWVPKKKKKPTWTSAGRQGGFSNGRMSLKMSSRTKAIYETATRRNLLQTDD--GGDDQ 242
           TKGWVPKKKKKPTW +AGRQGGF NGRMSLKMSSRT+AIYETATRRNLLQTDD  G DDQ
Sbjct: 182 TKGWVPKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTQAIYETATRRNLLQTDDDGGDDDQ 241

Query: 243 ISVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNKRYL 302
           I+VRDIVVVSQDG+GNFTTINEAIAAAPNNSAPTDGYFLIFV AGVYEEYV+IAKNKRYL
Sbjct: 242 IAVRDIVVVSQDGTGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVSIAKNKRYL 301

Query: 303 MMIGDGINQTVVTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRN 362
           MMIGDGINQT+VTGNR+VVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVA+RN
Sbjct: 302 MMIGDGINQTIVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVALRN 361

Query: 363 SADLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCNIYPRLPMA 422
            ADLSTFYLCSFE YQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVV QNCNIYPRLPM+
Sbjct: 362 GADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMS 421

Query: 423 NQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNSGARVNTFLGRPWKEYSRTVYMQS 482
           NQFNAITAQGRTDPNQNTGTSI+NCRITAADDLANNSGA V TFLGRPWKEYSRTVYMQS
Sbjct: 422 NQFNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQS 481

Query: 483 FMDDLINPAGWRAWDGDFALNTLYYAEFGNFGPGSNTTERVNWVGFHLIDDTDAGNFTAG 542
           FMDDLINPAGWRAWDGDFALNT YYAEFGNFGPGSNT+ERVNW GFHLI+DTDAGNFTAG
Sbjct: 482 FMDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAG 541

Query: 543 NFLMADDWVPQTGVPYTSGLTE 563
           NF++ADDW+PQTGVPYTSGLTE
Sbjct: 542 NFVLADDWLPQTGVPYTSGLTE 558

BLAST of Carg07155 vs. ExPASy TrEMBL
Match: A0A6J1H5E4 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111460725 PE=3 SV=1)

HSP 1 Score: 996.5 bits (2575), Expect = 4.5e-287
Identity = 495/552 (89.67%), Postives = 522/552 (94.57%), Query Frame = 0

Query: 11  FYSLLLISLYITAAGISTSAAPTGSVCSSAPDPSYCKSALPNQTGNVYSYGRSSVRKSLS 70
           F SL+L++LYIT A I  SAA   SVC+S PDPSYCKS LPNQTGNVYSYGRSS RKSLS
Sbjct: 6   FTSLILLTLYITTAQIPLSAA-AASVCTSTPDPSYCKSTLPNQTGNVYSYGRSSFRKSLS 65

Query: 71  SSQKFLRLVEKYLRSRSSLTVAAVRALEDCQLLAGLNIDYLTTSFRTVNSTSWTLPEMKA 130
           SSQKFLRLV+KYLRSRSSLTV AVRALEDCQLLAGLNIDYLTTSFRTVN+TS  L EMKA
Sbjct: 66  SSQKFLRLVKKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRTVNTTSSKLSEMKA 125

Query: 131 EDVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLAFFTKGWVPKK 190
           +DVQSLLSAILTNQQTCLDGLKATAGSWS+KNGLSLPLA+DTKLYSMSLA FTKGWVPKK
Sbjct: 126 DDVQSLLSAILTNQQTCLDGLKATAGSWSVKNGLSLPLASDTKLYSMSLALFTKGWVPKK 185

Query: 191 KKKPTWTSAGRQGGFSNGRMSLKMSSRTKAIYETATRRNLLQTDDGGDDQISVRDIVVVS 250
           KK+ TWT+AGRQGGF NGRMSLKMS+R KAIYE ATRRNLL+TDDGGDDQIS+RDIV+VS
Sbjct: 186 KKRSTWTAAGRQGGFRNGRMSLKMSNRAKAIYEMATRRNLLETDDGGDDQISIRDIVIVS 245

Query: 251 QDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNKRYLMMIGDGINQT 310
           QDGSGNFTTINEAIAAAPNNSAPTDGYFLIFV  GVYEEYV+IAKNKRYLMMIGDGINQT
Sbjct: 246 QDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSVGVYEEYVSIAKNKRYLMMIGDGINQT 305

Query: 311 VVTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNSADLSTFYLC 370
           V+TGNR+VVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRN ADLSTFYLC
Sbjct: 306 VITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLSTFYLC 365

Query: 371 SFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCNIYPRLPMANQFNAITAQG 430
           SFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVV QNCNIYPRLPMANQFNAITAQG
Sbjct: 366 SFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMANQFNAITAQG 425

Query: 431 RTDPNQNTGTSIHNCRITAADDLANNSGARVNTFLGRPWKEYSRTVYMQSFMDDLINPAG 490
           RTDPNQNTGTSIHNCRITAADDLANN+G  V TFLGRPWKEYSRTVY+QSFMD+LINPAG
Sbjct: 426 RTDPNQNTGTSIHNCRITAADDLANNTGVAVKTFLGRPWKEYSRTVYLQSFMDELINPAG 485

Query: 491 WRAWDGDFALNTLYYAEFGNFGPGSNTTERVNWVGFHLIDDTDAGNFTAGNFLMADDWVP 550
           WRAWD DFALNT YYAEFGNFGPGSNT+ERVNW+GFHLI+DTDAGNFTA NF++ADDW+P
Sbjct: 486 WRAWDKDFALNTSYYAEFGNFGPGSNTSERVNWLGFHLINDTDAGNFTAANFVLADDWLP 545

Query: 551 QTGVPYTSGLTE 563
           QTGVPYTSGLTE
Sbjct: 546 QTGVPYTSGLTE 556

BLAST of Carg07155 vs. TAIR 10
Match: AT1G02810.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 685.3 bits (1767), Expect = 4.2e-197
Identity = 345/573 (60.21%), Postives = 426/573 (74.35%), Query Frame = 0

Query: 12  YSLLLISLYI-TAAGISTSAAPTGSVCSSAPDPSYCKSALPNQTGNVYSYGRSSVRKSLS 71
           + L+ +S ++ +    S + + + ++C + PDP YCKS  P+  GNV  YG  S+RKSLS
Sbjct: 6   FILITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFPHSQGNVQQYGCFSIRKSLS 65

Query: 72  SSQKFLRLVEKYLRSRSSLT-VAAVRALEDCQLLAGLNIDYLTTSFRTVNSTS-----WT 131
            S+KF+R V++Y++  + L+  A +RAL+DC+ LAGL +DYL TSF TVN TS       
Sbjct: 66  QSRKFIRTVDRYIKRNAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAKTSFKP 125

Query: 132 LPEMKAEDVQSLLSAILTNQQTCLDGLKATA---GSWSLKNGLSLPLAADTKLYSMSLAF 191
           L   KA+D+Q+LLSA LTN+QTCL+GL   A    +W+++ G++LPL  DTKL  +SLA 
Sbjct: 126 LSFPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKLLGVSLAL 185

Query: 192 FTKGWVPKKKKKPTWTSAGRQGG----------FSNGRMSLKMSSRTKAIYETATRRNLL 251
           FTKGWVPKKKK+  +  A  + G          F NG + LKM+ +TKA+YE+ +RR L 
Sbjct: 186 FTKGWVPKKKKRAGFAWAQPRSGSSTHTKPFRLFRNGALPLKMTEKTKAVYESLSRRKLA 245

Query: 252 QTDDGGD----DQISVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVY 311
             D  GD      + + DIV VSQDG+GNFT I  A+AAAPNN+  + G+FLI+V AG+Y
Sbjct: 246 DGDSNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIY 305

Query: 312 EEYVTIAKNKRYLMMIGDGINQTVVTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNT 371
           EEY++IAKNKRY+MMIGDGINQTVVTGNR+VVDGWTTFNSATFAV  P FVAVN+TFRNT
Sbjct: 306 EEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRNT 365

Query: 372 AGAIKHQAVAVRNSADLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVV 431
           AG  KHQAVA+R+ AD S FY CSFE YQDTLYTHSLRQFYR+CD+YGTVDFIFGNAAVV
Sbjct: 366 AGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVV 425

Query: 432 LQNCNIYPRLPMANQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNSGARVNTFLGR 491
            QNCN+YPR PM NQFNAITAQGR+DPNQNTGTSI NC I  ADDL  +S   V T+LGR
Sbjct: 426 FQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLV-SSNYTVKTYLGR 485

Query: 492 PWKEYSRTVYMQSFMDDLINPAGWRAWDGDFALNTLYYAEFGNFGPGSNTTERVNWVGFH 551
           PWKEYSRTVYMQS++D  + P GWR W+GDFAL+TLYYAE+ N GPGSNTT RV W G+H
Sbjct: 486 PWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYH 545

Query: 552 LIDDTDAGNFTAGNFLMADDWVPQTGVPYTSGL 561
           +I+ TDA NFT     +  DW+ +TGVPYTSGL
Sbjct: 546 VINSTDAANFTVTGLFIEADWIWKTGVPYTSGL 577

BLAST of Carg07155 vs. TAIR 10
Match: AT4G02330.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 675.6 bits (1742), Expect = 3.4e-194
Identity = 350/568 (61.62%), Postives = 432/568 (76.06%), Query Frame = 0

Query: 14  LLLISLYITAAGI---STSAAPTGS---VCSSAPDPSYCKSALPNQT-GNVYSYGRSSVR 73
           L L++L+++   +   S +  P+ S   +C + PDP +CKS  P  + G+V  YGR S+R
Sbjct: 6   LFLVTLFLSLQTLFIASQTLLPSNSSSTICKTTPDPKFCKSVFPQTSQGDVREYGRFSLR 65

Query: 74  KSLSSSQKFLRLVEKYL-RSRSSLTVAAVRALEDCQLLAGLNIDYLTTSFRTVNST--SW 133
           KSL+ S+KF R +++YL R+ + L+ +AV AL+DC+ LA L  DYL TSF TVN T  S 
Sbjct: 66  KSLTQSRKFTRTIDRYLKRNNALLSQSAVGALQDCRYLASLTTDYLITSFETVNITTSSK 125

Query: 134 TLPEMKAEDVQSLLSAILTNQQTCLDGLK-ATAGSWSLKNGLSLPLAADTKLYSMSLAFF 193
           TL   KA+++Q+LLSA LTN+QTCLDG+  A + SW+++NG++LPL  DTKL+S+SLA F
Sbjct: 126 TLSFSKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLFSVSLALF 185

Query: 194 TKGWVPKKKK----------KPTWTSAGRQGGFSNGRMSLKMSSRTKAIYETATRRNLLQ 253
           TKGWVPKKKK          K T +       F NG + LKM+  T+A+YE+ +RR L  
Sbjct: 186 TKGWVPKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESLSRRKLAD 245

Query: 254 TDDGGDDQISVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVT 313
            DD   + + V DIV V+Q+G+GNFTTI EA+ +APN +  T GYF+I+V +GVYEE V 
Sbjct: 246 -DDNDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENVV 305

Query: 314 IAKNKRYLMMIGDGINQTVVTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIK 373
           IAKNKRYLMMIGDGIN+TVVTGNRNVVDGWTTFNSATFAV  P FVAVNMTFRNTAG  K
Sbjct: 306 IAKNKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTAGPEK 365

Query: 374 HQAVAVRNSADLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCN 433
           HQAVA+R+SADLS FY CSFE YQDTLYTHSLRQFYR+CDIYGTVDFIFGNAAVV Q+CN
Sbjct: 366 HQAVAMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDCN 425

Query: 434 IYPRLPMANQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNSGARVNTFLGRPWKEY 493
           +YPR PM NQFNAITAQGRTDPNQNTG SIHNC I  ADDL  +S   V T+LGRPWKEY
Sbjct: 426 LYPRQPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLV-SSNYTVKTYLGRPWKEY 485

Query: 494 SRTVYMQSFMDDLINPAGWRAWDGDFALNTLYYAEFGNFGPGSNTTERVNWVGFHLIDDT 553
           SRTV+MQS++D+++ P GWR W+GDFAL+TLYYAE+ N G GS+TT+RV W G+H+I+ T
Sbjct: 486 SRTVFMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVINST 545

Query: 554 DAGNFTAGNFLMADDWVPQTGVPYTSGL 561
           DA NFT  NFL+ D W+ Q+GVPY SGL
Sbjct: 546 DANNFTVENFLLGDGWMVQSGVPYISGL 571

BLAST of Carg07155 vs. TAIR 10
Match: AT2G47550.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 656.4 bits (1692), Expect = 2.1e-188
Identity = 332/549 (60.47%), Postives = 414/549 (75.41%), Query Frame = 0

Query: 17  ISLYITAAGISTSAAPTGSVCSSAPDPSYCKSALPNQTGNVYSYGRSSVRKSLSSSQKFL 76
           IS  I A G +TS +   ++C  APDPSYC+S LPNQ G++YSYGR S+R+SLS +++F+
Sbjct: 22  ISAQIPAIGNATSPS---NICRFAPDPSYCRSVLPNQPGDIYSYGRLSLRRSLSRARRFI 81

Query: 77  RLVEKYLRSRSSLTV-AAVRALEDCQLLAGLNIDYLTTSFRTVNSTSWTLPEMKAEDVQS 136
            +++  L  +  +   + V ALEDC+ LA L +DYL +S +T +ST  TL   +AEDV +
Sbjct: 82  SMIDAELDRKGKVAAKSTVGALEDCKFLASLTMDYLLSSSQTADSTK-TLSLSRAEDVHT 141

Query: 137 LLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLAFFTKGWVPKKKK-KP 196
            LSA +TN+QTCL+GLK+TA     +NGLS  L  DTKLY +SLA F+KGWVP++++ +P
Sbjct: 142 FLSAAITNEQTCLEGLKSTAS----ENGLSGDLFNDTKLYGVSLALFSKGWVPRRQRSRP 201

Query: 197 TWTSAGRQG---GFSNGRMSLKMSSRTKAIYETATRRNLLQTDDGGDDQISVRDIVVVSQ 256
            W    R     GF NG++ LKM+ R +A+Y T TRR LLQ+D    D + V DIV V Q
Sbjct: 202 IWQPQARFKKFFGFRNGKLPLKMTERARAVYNTVTRRKLLQSD---ADAVQVSDIVTVIQ 261

Query: 257 DGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNKRYLMMIGDGINQTV 316
           +G+GNFTTIN AIAAAPN +  ++GYFLI+V AG+YEEYV + KNKRY+MMIGDGINQTV
Sbjct: 262 NGTGNFTTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDGINQTV 321

Query: 317 VTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNSADLSTFYLCS 376
           +TGNR+VVDGWTTFNSATF + GP F+ VN+T RNTAG  K QAVA+R+  DLS FY CS
Sbjct: 322 ITGNRSVVDGWTTFNSATFILSGPNFIGVNITIRNTAGPTKGQAVALRSGGDLSVFYSCS 381

Query: 377 FEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCNIYPRLPMANQFNAITAQGR 436
           FE YQDTLYTHSLRQFYR+CD+YGTVDFIFGNAAVVLQNCN+YPR P   Q N +TAQGR
Sbjct: 382 FEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQSNEVTAQGR 441

Query: 437 TDPNQNTGTSIHNCRITAADDLANNSGARVNTFLGRPWKEYSRTVYMQSFMDDLINPAGW 496
           TDPNQNTGT+IH C I  ADDLA  S   V T+LGRPWKEYSRTV MQ+++D  + P+GW
Sbjct: 442 TDPNQNTGTAIHGCTIRPADDLA-TSNYTVKTYLGRPWKEYSRTVVMQTYIDGFLEPSGW 501

Query: 497 RAWDGDFALNTLYYAEFGNFGPGSNTTERVNWVGFHLIDDTDAGNFTAGNFLMADDWVPQ 556
            AW GDFAL+TLYYAE+ N GPGS+TT RV W G+H+I+ TDA NFT  NFL+ + W+ Q
Sbjct: 502 NAWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYHVINATDASNFTVTNFLVGEGWIGQ 558

Query: 557 TGVPYTSGL 561
           TGVP+  GL
Sbjct: 562 TGVPFVGGL 558

BLAST of Carg07155 vs. TAIR 10
Match: AT3G10720.2 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 491.1 bits (1263), Expect = 1.2e-138
Identity = 263/549 (47.91%), Postives = 352/549 (64.12%), Query Frame = 0

Query: 27  STSAAPTGSVCSSAPDPSYCK---SALPNQTGNVYSYGRSSVRKSLSSSQKFLRLVEKYL 86
           S S +P+   C S P P  C+   +A+ +   + Y YG+ ++++ L  + +  +++  Y 
Sbjct: 74  SQSQSPS-LACKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVITSYA 133

Query: 87  R----SRSSLTVAAVRALEDCQLLAGLNIDYLTTSFRTVNSTSWTLPEMKAEDVQSLLSA 146
           R       S T   + A+ DC  L+ L+++YL T   T   T+  +     E V SLLS 
Sbjct: 134 RRVESKPGSATAEEIGAVADCGELSELSVNYLET-VTTELKTAQVMTAALVEHVNSLLSG 193

Query: 147 ILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLAFFTKGWVPKKKKKPTWTSA 206
           ++TNQQTCLDGL      ++    +  P+   T+LYS+SL   +      +  K    S 
Sbjct: 194 VVTNQQTCLDGLVEAKSGFAA--AIGSPMGNLTRLYSISLGLVSHAL--NRNLKRFKASK 253

Query: 207 GRQGGFSNGRMSLKMSSRTKAIYETA--------TRRNLLQTDDGGDDQISVRDIVVVSQ 266
           G+  G  N      + +  K + +T         T RNL +  +     I V   V+V  
Sbjct: 254 GKILGGGNSTYREPLETLIKGLRKTCDNDKDCRKTSRNLGELGETSGGSILVSKAVIVGP 313

Query: 267 DGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNKRYLMMIGDGINQTV 326
             S NFTTI +AIAAAPNN+ P DGYF+I+   GVYEEY+ +  NK+ LM++GDGIN+T+
Sbjct: 314 FKSDNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINKTI 373

Query: 327 VTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNSADLSTFYLCS 386
           +TGN NVVDGWTT+N ++FAVVG  F+AV++TFRNTAG  KHQAVA+RN+A+ S+FY CS
Sbjct: 374 ITGNHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYRCS 433

Query: 387 FEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCNIYPRLPMANQFNAITAQGR 446
           FEGYQDTLY HSLRQFYR+CDIYGTVDFIFGNAA + QNCNIY R PMA Q NAITA GR
Sbjct: 434 FEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAHGR 493

Query: 447 TDPNQNTGTSIHNCRITAADDLANNSGARVNTFLGRPWKEYSRTVYMQSFMDDLINPAGW 506
            DPNQNTG SI NC I AA DLA    + + TFLGRPWK YSRTV+MQS++ D++ P GW
Sbjct: 494 LDPNQNTGISIINCTIKAAPDLAAEPKSAM-TFLGRPWKPYSRTVFMQSYISDIVQPVGW 553

Query: 507 RAWDGDFALNTLYYAEFGNFGPGSNTTERVNWVGFHLIDDTDAGNFTAGNFLMADDWVPQ 561
             W+G   L+T+YY E+ NFGPG+NT +RV W+G++L++  +A NFT  NF M D W+PQ
Sbjct: 554 LEWNGTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTMGDTWLPQ 613

BLAST of Carg07155 vs. TAIR 10
Match: AT5G04970.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 479.9 bits (1234), Expect = 2.7e-135
Identity = 256/547 (46.80%), Postives = 350/547 (63.99%), Query Frame = 0

Query: 29  SAAPTGSVCSSAPDPSYCK---SALPNQTGNVYSYGRSSVRKSLSSSQKFLRLVEKYLR- 88
           S  P    C S P P  C+   SA+ +   + Y YG+ ++++ L  +++  +++ ++ + 
Sbjct: 80  SPLPPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVINRFAQR 139

Query: 89  ---SRSSLTVAAVRALEDCQLLAGLNIDYLTTSFRTVNSTSWTLPEMKAEDVQSLLSAIL 148
                 + TV  V A+ DC  LA L+++YL T    + +    +     + V SLL  ++
Sbjct: 140 VEADPGTSTVEEVSAVADCGELAELSVEYLETVTEELKAAE-LMTAALVDRVTSLLGGVV 199

Query: 149 TNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLAFFTKGWVPKKKKKPTWTSAGR 208
           TNQQTCLDGL      ++    +  PL   T+LYS+SL   +      +  K    S G+
Sbjct: 200 TNQQTCLDGLVDAKSGFA--TAIGTPLGNLTRLYSVSLGLVSHAL--NRNLKRYKGSKGK 259

Query: 209 QGGFSNGRMSLKMSSRTKAIYETATR--------RNLLQTDDGGDDQISVRDIVVVSQDG 268
             G  N  +   + +  K + +T  +        RNL +  +     I VR+ V V    
Sbjct: 260 IFGGGNKPVREPLETLIKVLRKTCDKGKDCRKANRNLGELGETSGGSILVREAVTVGPYE 319

Query: 269 SGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNKRYLMMIGDGINQTVVT 328
           + NF TI EA+AAAPN++ P  GYF+I+  AG+YEEYV I+  KR +M+IGDGIN+T+++
Sbjct: 320 TDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINKTIIS 379

Query: 329 GNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNSADLSTFYLCSFE 388
           GN + +DGWTT+NS+TFAVVG  FVAV++TFRNTAG  KHQAVAVRN+AD STFY CSFE
Sbjct: 380 GNHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYRCSFE 439

Query: 389 GYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCNIYPRLPMANQFNAITAQGRTD 448
           GYQDTLY HSLRQFYR+CDIYGT+DFIFGNAA + QNCNIY R PMANQ NA+TA GRTD
Sbjct: 440 GYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAHGRTD 499

Query: 449 PNQNTGTSIHNCRITAADDLANNSGARVNTFLGRPWKEYSRTVYMQSFMDDLINPAGWRA 508
           PNQ TG SI NC I AA DLA +  + + TFLGRPWK YSRTVY+QS++ D++ P GW  
Sbjct: 500 PNQKTGISIINCTIGAAPDLAADPKSTM-TFLGRPWKPYSRTVYIQSYISDVVQPVGWLE 559

Query: 509 WDGDFALNTLYYAEFGNFGPGSNTTERVNWVGFHLIDDTDAGNFTAGNFLMADDWVPQTG 561
           W+G   L+T+ Y E+ NFGPG++T++RV W G+ L++   A NFT  NF + D W+PQT 
Sbjct: 560 WNGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLLNLVQAMNFTVYNFTLGDTWLPQTD 619

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7014346.10.0e+00100.00putative pectinesterase/pectinesterase inhibitor 41, partial [Cucurbita argyrosp... [more]
KAG6575804.10.0e+0099.64putative pectinesterase/pectinesterase inhibitor 41, partial [Cucurbita argyrosp... [more]
XP_022953705.10.0e+0099.29probable pectinesterase/pectinesterase inhibitor 7 [Cucurbita moschata][more]
XP_023548976.10.0e+0099.29probable pectinesterase/pectinesterase inhibitor 7 isoform X1 [Cucurbita pepo su... [more]
XP_022991223.10.0e+0098.04probable pectinesterase/pectinesterase inhibitor 7 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9SRX46.0e-19660.21Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=37... [more]
Q8RXK74.7e-19361.62Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3... [more]
O222563.0e-18760.47Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3... [more]
Q94CB11.7e-13747.91Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3... [more]
Q9FF773.8e-13446.80Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A6J1GQE60.0e+0099.29Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111456155 PE=3 SV=1[more]
A0A6J1JVJ50.0e+0098.04Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111487941 PE=3 SV=1[more]
A0A5D3D3H43.0e-29190.39Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G00132... [more]
A0A1S3BSI23.0e-29190.39Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492791 PE=3 SV=1[more]
A0A6J1H5E44.5e-28789.67Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111460725 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G02810.14.2e-19760.21Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G02330.13.4e-19461.62Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT2G47550.12.1e-18860.47Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G10720.21.2e-13847.91Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G04970.12.7e-13546.80Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 27..181
e-value: 7.8E-21
score: 85.2
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 35..180
e-value: 6.2E-16
score: 59.1
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 33..184
e-value: 8.0E-20
score: 73.4
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 35..184
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 247..545
e-value: 3.2E-136
score: 452.9
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 241..560
e-value: 4.2E-119
score: 399.6
NoneNo IPR availablePANTHERPTHR31707:SF294PECTINESTERASE/PECTINESTERASE INHIBITOR 7-RELATEDcoord: 24..560
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 24..560
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 35..186
e-value: 3.41497E-26
score: 102.516
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 393..402
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 245..559

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg07155-RACarg07155-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity