Carg07106 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg07106
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	nifU-like protein 1, chloroplastic
Location	Carg_Chr17: 7819518 .. 7821785 (-)
RNA-Seq Expression	Carg07106
Synteny	Carg07106

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CAAGTTCGTCATCATCGGCCAGATTCCTTTTCATTTCTTATTCATCAATGGCGTCTTTAACTATCTCCGGCCTCCTCAAAACCCCTCTTTATTATTCCCAAGCTCTTGCAAATCACCGCCAGTACCCACCTTCTGCTTTTGGGCAACATCAAATACCCGTTTCCCTGAATAGGGTGTTGCCCAGACAAGCCATTAGAGCTTCCGCTTCGAGTTCTGGTCCATCCACGAGCTCCTCGCCAGGGCTGTACTCCGCCCAGAAATTCGAACTGACCACTGCAAACGTAGACTTGGTTCTTGAGGACGTTAGGCCCTATCTAATCGCAGATGGAGGAAATGTCGATGTCGTGTCCGTTGAAGATGGAATCGTTTCTCTCAAACTCGTTGGTGGGTGAATTCCTGATCTCATTTCATTGAATGGGTTTGTTTTAATCTTGTTTGTTAATTTAGCTTTGTGTATTGAAATTTTTGTAATTGAATGATTGGTTGCTACTGCTTATGTGCGCGAAATGTTGATGAACAATCGAATAGGTTAGGGAGTTTATGCCTCAGAAAATTCTAAATTCTTCATTTGGGCGTTTCTTAGACCAAGAGTTATAGGATCCCTAGAAGTTGAAGACATCAAAGAACTCTTAGTTCCATGGCCTTGGTACTTAGAAGCTCAAACACCACTTCATGAAGATACTGTATCCTTTTACCATCCAGCTTCTCAATTCTTATATGTTTAGGTTCTGATCATATCTTTTGCTCTCTGCCCCATTGCCTAGAGTTTTCAGGAGAGTTTTTTTTGGGAGATCATATTTGATCATATTCCATAGACCCACCATCTCCCCGGTACAATTTGGCTCAGCTCGTAACATCCAAACCCACCGCTAACAGATATTATCCACTTTGGTCCGTTATGTATCGCTGTCAGCCTCATGGAGTTAAAACGCGTCTATTAGAGAGAGGTTTCCACACCCTTATAAGGAGTGCTTCGTTCCCTCTCCAACCGATGTGGGATCTCACAATCCACCCCCCTTGGGGGCCTACGTCTTCACTAGTACAGTACTCGGTGTTTGGCTCTAATACCATTTGTAACAGTCCAAGCCCACCGCTAACAAATATTGTCTACTTTAGCTTGTTACGGACCGTTGTCAGTCTCAAAGTTTTAAAACGTGTCTGCTAGGGAGAGGTTTTCACGTTCTTATGAAAAATGTTTCGTTCCTCTCTCCAACCGATGTGAGATCTCACGCAGCTAGTTTAAAACTTTTCCTTATTGACTAGTTGAAGAGCATTTATTTGTTATCTCGGTCCAGACTGGATTTGGACCTCTAGTACCTGCCTCGACACGTGCCCGGTGAGTTTATCTGTTTCATCTGCTGTAGGGGAAAACGACTCCTTGAAGTAGGTGGGCAAAAGGTAGTGAGCATGTAATCTTCACTATCTTGTTAGACATCTTGCAGCAGATACCTGAGTTTATCTTTGAGTTTTGAGCTTAGATTCCATTTAGTCCATAAGCTTCAAGATTTTACACTTTTTTTTTTAACTAAAGACTTAGCAAGAATCAAATCAATCCTCAGGAGCATGCGGTAGCTGCCCGAGCTCAACAACCACCATGAAGATGGGAATCGAACGAGTACTGAAGGAAAAGTTTGGAGATTCAGTGAAGGAAATATGCCAAGTATATGATGAAGAGCCAAAGGAAATAACAACCGAGGTGAGTTTTCTCTGGACGAACGAACGAATACAAAAGAACTCGAACATATTTCGATGTTATGTTAAATTCAAACAAAAACAGAGAGATGAATTAGTATTAACGTTGGATGTTGCATCAATGCAGGCAGTAAATAGTCATCTTGACATATTGAGGCCAGCCATAAGGAACTATGGCGGGAGCGTGGAAGTAATATCGATCGATGGTGGCAATTGCGTCGTTAAGTATGAGGGTCCTGAATCTATTGGTACAGGAATTAAAGCAGCCATTAAGGAGAAGTTCCCAGATATTACAAACGTTGTGTTTTCAAGCTAGCCTTTTCTCAAGTTCATTTAGCTGCTGCATTTAATGAACAGGTGCTGCTCCTCTTTCCAGAATACCCACATGTAAAGTTCTATTGTTCAATAACTGATACATATATGACTATAACTTATGCCCAAAAAGGCTCGTACATATTACCGTCAGTCTTACAGTTTTAAAACACTGGGAGAGGTTTTCATACCCTTATAAGGAATGTTTCGTTCCCTTCTCTAACTGATGTAGGATCTCGTAGAAAGTAAACTTTTTTAAAAAGG

mRNA sequence

CAAGTTCGTCATCATCGGCCAGATTCCTTTTCATTTCTTATTCATCAATGGCGTCTTTAACTATCTCCGGCCTCCTCAAAACCCCTCTTTATTATTCCCAAGCTCTTGCAAATCACCGCCAGTACCCACCTTCTGCTTTTGGGCAACATCAAATACCCGTTTCCCTGAATAGGGTGTTGCCCAGACAAGCCATTAGAGCTTCCGCTTCGAGTTCTGGTCCATCCACGAGCTCCTCGCCAGGGCTGTACTCCGCCCAGAAATTCGAACTGACCACTGCAAACGTAGACTTGGTTCTTGAGGACGTTAGGCCCTATCTAATCGCAGATGGAGGAAATGTCGATGTCGTGTCCGTTGAAGATGGAATCGTTTCTCTCAAACTCGTTGGAGCATGCGGTAGCTGCCCGAGCTCAACAACCACCATGAAGATGGGAATCGAACGAGTACTGAAGGAAAAGTTTGGAGATTCAGTGAAGGAAATATGCCAAGTATATGATGAAGAGCCAAAGGAAATAACAACCGAGGCAGTAAATAGTCATCTTGACATATTGAGGCCAGCCATAAGGAACTATGGCGGGAGCGTGGAAGTAATATCGATCGATGGTGGCAATTGCGTCGTTAAGTATGAGGGTCCTGAATCTATTGGTACAGGAATTAAAGCAGCCATTAAGGAGAAGTTCCCAGATATTACAAACGTTGTGTTTTCAAGCTAGCCTTTTCTCAAGTTCATTTAGCTGCTGCATTTAATGAACAGGTGCTGCTCCTCTTTCCAGAATACCCACATGTAAAGTTCTATTGTTCAATAACTGATACATATATGACTATAACTTATGCCCAAAAAGGCTCGTACATATTACCGTCAGTCTTACAGTTTTAAAACACTGGGAGAGGTTTTCATACCCTTATAAGGAATGTTTCGTTCCCTTCTCTAACTGATGTAGGATCTCGTAGAAAGTAAACTTTTTTAAAAAGG

Coding sequence (CDS)

ATGGCGTCTTTAACTATCTCCGGCCTCCTCAAAACCCCTCTTTATTATTCCCAAGCTCTTGCAAATCACCGCCAGTACCCACCTTCTGCTTTTGGGCAACATCAAATACCCGTTTCCCTGAATAGGGTGTTGCCCAGACAAGCCATTAGAGCTTCCGCTTCGAGTTCTGGTCCATCCACGAGCTCCTCGCCAGGGCTGTACTCCGCCCAGAAATTCGAACTGACCACTGCAAACGTAGACTTGGTTCTTGAGGACGTTAGGCCCTATCTAATCGCAGATGGAGGAAATGTCGATGTCGTGTCCGTTGAAGATGGAATCGTTTCTCTCAAACTCGTTGGAGCATGCGGTAGCTGCCCGAGCTCAACAACCACCATGAAGATGGGAATCGAACGAGTACTGAAGGAAAAGTTTGGAGATTCAGTGAAGGAAATATGCCAAGTATATGATGAAGAGCCAAAGGAAATAACAACCGAGGCAGTAAATAGTCATCTTGACATATTGAGGCCAGCCATAAGGAACTATGGCGGGAGCGTGGAAGTAATATCGATCGATGGTGGCAATTGCGTCGTTAAGTATGAGGGTCCTGAATCTATTGGTACAGGAATTAAAGCAGCCATTAAGGAGAAGTTCCCAGATATTACAAACGTTGTGTTTTCAAGCTAG

Protein sequence

MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNRVLPRQAIRASASSSGPSTSSSPGLYSAQKFELTTANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPSSTTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEVISIDGGNCVVKYEGPESIGTGIKAAIKEKFPDITNVVFSS

Homology

[Close]

BLAST of Carg07106 vs. NCBI nr
Match: XP_022953177.1 (nifU-like protein 1, chloroplastic [Cucurbita moschata] >KAG6575747.1 NifU-like protein 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7014298.1 NifU-like protein 1, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 429.5 bits (1103), Expect = 1.8e-116
Identity = 220/220 (100.00%), Postives = 220/220 (100.00%), Query Frame = 0


Query: 1   MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNRVLPRQAIRASASSSGPST 60
           MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNRVLPRQAIRASASSSGPST
Sbjct: 1   MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNRVLPRQAIRASASSSGPST 60

Query: 61  SSSPGLYSAQKFELTTANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS 120
           SSSPGLYSAQKFELTTANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS
Sbjct: 61  SSSPGLYSAQKFELTTANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV 180

Query: 181 ISIDGGNCVVKYEGPESIGTGIKAAIKEKFPDITNVVFSS 221
           ISIDGGNCVVKYEGPESIGTGIKAAIKEKFPDITNVVFSS
Sbjct: 181 ISIDGGNCVVKYEGPESIGTGIKAAIKEKFPDITNVVFSS 220

[Close]

BLAST of Carg07106 vs. NCBI nr
Match: XP_022991837.1 (nifU-like protein 1, chloroplastic [Cucurbita maxima])

HSP 1 Score: 421.0 bits (1081), Expect = 6.3e-114
Identity = 215/220 (97.73%), Postives = 217/220 (98.64%), Query Frame = 0


Query: 1   MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNRVLPRQAIRASASSSGPST 60
           MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNR LPRQ+IRASAS+SGPST
Sbjct: 1   MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNRALPRQSIRASASNSGPST 60

Query: 61  SSSPGLYSAQKFELTTANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS 120
           SSSPGLYSAQKFELT ANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS
Sbjct: 61  SSSPGLYSAQKFELTIANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV 180

Query: 181 ISIDGGNCVVKYEGPESIGTGIKAAIKEKFPDITNVVFSS 221
           ISIDGGNCVV YEGPESIGTGIKAAIKEKFPDITNVVFSS
Sbjct: 181 ISIDGGNCVVMYEGPESIGTGIKAAIKEKFPDITNVVFSS 220

[Close]

BLAST of Carg07106 vs. NCBI nr
Match: XP_023547622.1 (nifU-like protein 1, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 420.2 bits (1079), Expect = 1.1e-113
Identity = 215/220 (97.73%), Postives = 216/220 (98.18%), Query Frame = 0


Query: 1   MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNRVLPRQAIRASASSSGPST 60
           MASLTISGLLKTPLYYSQALANHRQYPP +FGQHQIPV LNR LP QAIRASASSSGPST
Sbjct: 1   MASLTISGLLKTPLYYSQALANHRQYPPFSFGQHQIPVCLNRALPSQAIRASASSSGPST 60

Query: 61  SSSPGLYSAQKFELTTANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS 120
           SSSPGLYSAQKFELTTANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS
Sbjct: 61  SSSPGLYSAQKFELTTANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV 180

Query: 181 ISIDGGNCVVKYEGPESIGTGIKAAIKEKFPDITNVVFSS 221
           ISIDGGNCVVKYEGPESIGTGIKAAIKEKFPDITNVVFSS
Sbjct: 181 ISIDGGNCVVKYEGPESIGTGIKAAIKEKFPDITNVVFSS 220

[Close]

BLAST of Carg07106 vs. NCBI nr
Match: XP_022144330.1 (nifU-like protein 1, chloroplastic [Momordica charantia])

HSP 1 Score: 380.6 bits (976), Expect = 9.4e-102
Identity = 195/219 (89.04%), Postives = 204/219 (93.15%), Query Frame = 0


Query: 1   MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNRVLPRQAIRASASSSGPST 60
           MASLT SGLLKTP  YSQ  AN+RQYPPSA  QHQ P SL RVLPR+ I+ASAS+SGPST
Sbjct: 1   MASLTTSGLLKTPPCYSQIPANYRQYPPSAHRQHQGPNSLKRVLPRRGIKASASNSGPST 60

Query: 61  SSSPGLYSAQKFELTTANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS 120
           SSSPGLYSAQKFELT  NVDLVLEDVRPYLIADGGNVDVVSVEDG+VSLKLVGACGSCPS
Sbjct: 61  SSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKE T EAVNSHLD+LRPAIRNYGGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDVLRPAIRNYGGSVEV 180

Query: 181 ISIDGGNCVVKYEGPESIGTGIKAAIKEKFPDITNVVFS 220
           ISIDGG+C+VKYEGPESIGTGIKAAIKE+FPDITNVVFS
Sbjct: 181 ISIDGGDCLVKYEGPESIGTGIKAAIKERFPDITNVVFS 219

[Close]

BLAST of Carg07106 vs. NCBI nr
Match: XP_008451442.1 (PREDICTED: nifU-like protein 1, chloroplastic [Cucumis melo])

HSP 1 Score: 379.8 bits (974), Expect = 1.6e-101
Identity = 192/220 (87.27%), Postives = 205/220 (93.18%), Query Frame = 0


Query: 1   MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNRVLPRQAIRASASSSGPST 60
           MASLT SGLLKTPL  SQ+ AN+ QYPP A+GQHQ+PVSL R  P +A+RAS S+SGPST
Sbjct: 1   MASLTTSGLLKTPLKNSQSPANYFQYPPFAYGQHQLPVSLKRAFPGRAVRASPSNSGPST 60

Query: 61  SSSPGLYSAQKFELTTANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS 120
           SSSPGLYSAQKFELT  NVDLVLEDVRPYLIADGGNVDVVSVEDG+VSLKLVGACGSCPS
Sbjct: 61  SSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKE T EAVN+HLDILRPAIRNYGGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNNHLDILRPAIRNYGGSVEV 180

Query: 181 ISIDGGNCVVKYEGPESIGTGIKAAIKEKFPDITNVVFSS 221
           ISI+GGNC+VKYEGPESIGTG+KAAIKE+FPDITNVVFSS
Sbjct: 181 ISINGGNCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS 220

[Close]

BLAST of Carg07106 vs. ExPASy Swiss-Prot
Match: Q93W77 (NifU-like protein 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NIFU1 PE=1 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 6.6e-66
Identity = 134/195 (68.72%), Postives = 156/195 (80.00%), Query Frame = 0


Query: 31  FGQHQIPVSLNRVLPRQAIRASASSSGPSTSS-----SPGLYSAQKFELTTANVDLVLED 90
           F   +  +S   +  R AI  S SS G   S      S GLYSAQ F+LT  NVDLVLED
Sbjct: 39  FANKRRHISRTAIFHRSAI--SGSSQGEKISPLASGVSSGLYSAQTFDLTPQNVDLVLED 98

Query: 91  VRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPSSTTTMKMGIERVLKEKFGDSVKEIC 150
           VRP+LI+DGGNVDVVSVEDG+VSLKL GAC SCPSS+TTM MGIERVLKEKFGD++K+I 
Sbjct: 99  VRPFLISDGGNVDVVSVEDGVVSLKLQGACTSCPSSSTTMTMGIERVLKEKFGDALKDIR 158

Query: 151 QVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEVISIDGGNCVVKYEGPESIGTGIKAA 210
           QV+DEE K+IT EAVN+HLDILRPAI+NYGGSVEV+S++G +CVVKY GPESIG GI+AA
Sbjct: 159 QVFDEEVKQITVEAVNAHLDILRPAIKNYGGSVEVLSVEGEDCVVKYVGPESIGMGIQAA 218

Query: 211 IKEKFPDITNVVFSS 221
           IKEKF DI+NV F+S
Sbjct: 219 IKEKFKDISNVTFTS 231

[Close]

BLAST of Carg07106 vs. ExPASy Swiss-Prot
Match: Q93W20 (NifU-like protein 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NIFU2 PE=1 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 8.5e-21
Identity = 58/148 (39.19%), Postives = 86/148 (58.11%), Query Frame = 0


Query: 74  LTTANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPSSTTTMKMGIERVL 133
           LT  NV+ VL+++RPYL++DGGNV +  ++  IV +KL GACGSCPSST TMKMGIER L
Sbjct: 85  LTEENVESVLDEIRPYLMSDGGNVALHEIDGNIVRVKLQGACGSCPSSTMTMKMGIERRL 144

Query: 134 KEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRP-AIRNYGGSVEVISIDGGNCVVKY 193
            EK  + V       +E   E+  E +   L+ +RP  I    GS++++ I+     ++ 
Sbjct: 145 MEKIPEIVAVEALPDEETGLELNEENIEKVLEEIRPYLIGTADGSLDLVEIEDPIVKIRI 204

Query: 194 EGPE----SIGTGIKAAIKEKFPDITNV 217
            GP     ++   +   ++EK P I  V
Sbjct: 205 TGPAAGVMTVRVAVTQKLREKIPSIAAV 232

[Close]

BLAST of Carg07106 vs. ExPASy Swiss-Prot
Match: Q84LK7 (NifU-like protein 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=NIFU1 PE=1 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 2.7e-19
Identity = 62/152 (40.79%), Postives = 87/152 (57.24%), Query Frame = 0


Query: 68  SAQKFELTTANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPSSTTTMKM 127
           +A +  LT  NV+ VL+ VRPYL ADGG+V +  +   +V LKL GACGSCPSS  T+K 
Sbjct: 69  TAVQLPLTAGNVESVLDQVRPYLTADGGDVALHEIAGNVVRLKLQGACGSCPSSLITIKR 128

Query: 128 GIERVLKEKFGDSVKEICQVYDEEP-KEITTEAVNSHLDILRPAIRNY-GGSVEVISIDG 187
           GIER L EK  D V  +  V D+E   E+  E V   L+ +RP +    GG ++ + I G
Sbjct: 129 GIERRLMEKIPD-VAAVEPVTDKETGLELNEENVEKVLNEIRPYLAGTGGGGLQFLMIKG 188

Query: 188 GNCVVKYEGPESIGTGIKAAI----KEKFPDI 214
               V+  GP ++   ++ A+    +EK P I
Sbjct: 189 PIVKVRLTGPAAVVRTVRIAVSKKLREKIPSI 219

[Close]

BLAST of Carg07106 vs. ExPASy Swiss-Prot
Match: Q84RQ7 (NifU-like protein 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NIFU3 PE=2 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 2.3e-18
Identity = 56/139 (40.29%), Postives = 84/139 (60.43%), Query Frame = 0


Query: 74  LTTANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPSSTTTMKMGIERVL 133
           LT  NV+ VL++VRP L+ADGGNV +  ++  +V LKL GACGSCPSS+ T+KMGIE  L
Sbjct: 84  LTEENVERVLDEVRPSLMADGGNVALHEIDGLVVVLKLQGACGSCPSSSMTLKMGIESRL 143

Query: 134 KEKFGDSVKEICQVYDEEP--KEITTEAVNSHLDILRPAIRNY-GGSVEVISIDGGNCVV 193
           ++K  + +  + Q  + E    E+  E +   L  LRP +    GG +E++ IDG    V
Sbjct: 144 RDKIPE-IMSVEQFLESETGGLELNDENIEKVLSELRPYLSGTGGGGLELVEIDGYVVKV 203

Query: 194 KYEGPESIGTGIKAAIKEK 210
           +  GP +    ++ A+ +K
Sbjct: 204 RLTGPAAGVMTVRVALTQK 221

[Close]

BLAST of Carg07106 vs. ExPASy Swiss-Prot
Match: O32119 (Putative nitrogen fixation protein YutI OS=Bacillus subtilis (strain 168) OX=224308 GN=yutI PE=3 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 5.0e-13
Identity = 38/70 (54.29%), Postives = 53/70 (75.71%), Query Frame = 0


Query: 79  VDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPSSTTTMKMGIERVLKEKFG 138
           V  VL+ +RP+L+ DGG+ ++V V++GIV L+L+GACGSCPSST T+K GIER L E+  
Sbjct: 43  VQEVLDKLRPFLLRDGGDCELVDVDEGIVKLRLLGACGSCPSSTITLKAGIERALLEEV- 102

Query: 139 DSVKEICQVY 149
             V E+ QV+
Sbjct: 103 PGVVEVEQVF 111

[Close]

BLAST of Carg07106 vs. ExPASy TrEMBL
Match: A0A6J1GMI1 (nifU-like protein 1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111455795 PE=3 SV=1)

HSP 1 Score: 429.5 bits (1103), Expect = 8.5e-117
Identity = 220/220 (100.00%), Postives = 220/220 (100.00%), Query Frame = 0


Query: 1   MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNRVLPRQAIRASASSSGPST 60
           MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNRVLPRQAIRASASSSGPST
Sbjct: 1   MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNRVLPRQAIRASASSSGPST 60

Query: 61  SSSPGLYSAQKFELTTANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS 120
           SSSPGLYSAQKFELTTANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS
Sbjct: 61  SSSPGLYSAQKFELTTANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV 180

Query: 181 ISIDGGNCVVKYEGPESIGTGIKAAIKEKFPDITNVVFSS 221
           ISIDGGNCVVKYEGPESIGTGIKAAIKEKFPDITNVVFSS
Sbjct: 181 ISIDGGNCVVKYEGPESIGTGIKAAIKEKFPDITNVVFSS 220

[Close]

BLAST of Carg07106 vs. ExPASy TrEMBL
Match: A0A6J1JN01 (nifU-like protein 1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111488364 PE=3 SV=1)

HSP 1 Score: 421.0 bits (1081), Expect = 3.0e-114
Identity = 215/220 (97.73%), Postives = 217/220 (98.64%), Query Frame = 0


Query: 1   MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNRVLPRQAIRASASSSGPST 60
           MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNR LPRQ+IRASAS+SGPST
Sbjct: 1   MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNRALPRQSIRASASNSGPST 60

Query: 61  SSSPGLYSAQKFELTTANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS 120
           SSSPGLYSAQKFELT ANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS
Sbjct: 61  SSSPGLYSAQKFELTIANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV 180

Query: 181 ISIDGGNCVVKYEGPESIGTGIKAAIKEKFPDITNVVFSS 221
           ISIDGGNCVV YEGPESIGTGIKAAIKEKFPDITNVVFSS
Sbjct: 181 ISIDGGNCVVMYEGPESIGTGIKAAIKEKFPDITNVVFSS 220

[Close]

BLAST of Carg07106 vs. ExPASy TrEMBL
Match: A0A6J1CRB9 (nifU-like protein 1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111014036 PE=3 SV=1)

HSP 1 Score: 380.6 bits (976), Expect = 4.6e-102
Identity = 195/219 (89.04%), Postives = 204/219 (93.15%), Query Frame = 0


Query: 1   MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNRVLPRQAIRASASSSGPST 60
           MASLT SGLLKTP  YSQ  AN+RQYPPSA  QHQ P SL RVLPR+ I+ASAS+SGPST
Sbjct: 1   MASLTTSGLLKTPPCYSQIPANYRQYPPSAHRQHQGPNSLKRVLPRRGIKASASNSGPST 60

Query: 61  SSSPGLYSAQKFELTTANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS 120
           SSSPGLYSAQKFELT  NVDLVLEDVRPYLIADGGNVDVVSVEDG+VSLKLVGACGSCPS
Sbjct: 61  SSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKE T EAVNSHLD+LRPAIRNYGGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDVLRPAIRNYGGSVEV 180

Query: 181 ISIDGGNCVVKYEGPESIGTGIKAAIKEKFPDITNVVFS 220
           ISIDGG+C+VKYEGPESIGTGIKAAIKE+FPDITNVVFS
Sbjct: 181 ISIDGGDCLVKYEGPESIGTGIKAAIKERFPDITNVVFS 219

[Close]

BLAST of Carg07106 vs. ExPASy TrEMBL
Match: A0A1S3BQX1 (nifU-like protein 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103492737 PE=3 SV=1)

HSP 1 Score: 379.8 bits (974), Expect = 7.8e-102
Identity = 192/220 (87.27%), Postives = 205/220 (93.18%), Query Frame = 0


Query: 1   MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNRVLPRQAIRASASSSGPST 60
           MASLT SGLLKTPL  SQ+ AN+ QYPP A+GQHQ+PVSL R  P +A+RAS S+SGPST
Sbjct: 1   MASLTTSGLLKTPLKNSQSPANYFQYPPFAYGQHQLPVSLKRAFPGRAVRASPSNSGPST 60

Query: 61  SSSPGLYSAQKFELTTANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS 120
           SSSPGLYSAQKFELT  NVDLVLEDVRPYLIADGGNVDVVSVEDG+VSLKLVGACGSCPS
Sbjct: 61  SSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKE T EAVN+HLDILRPAIRNYGGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNNHLDILRPAIRNYGGSVEV 180

Query: 181 ISIDGGNCVVKYEGPESIGTGIKAAIKEKFPDITNVVFSS 221
           ISI+GGNC+VKYEGPESIGTG+KAAIKE+FPDITNVVFSS
Sbjct: 181 ISINGGNCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS 220

[Close]

BLAST of Carg07106 vs. ExPASy TrEMBL
Match: A0A5A7TT28 (NifU-like protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G001900 PE=3 SV=1)

HSP 1 Score: 377.9 bits (969), Expect = 3.0e-101
Identity = 191/220 (86.82%), Postives = 205/220 (93.18%), Query Frame = 0


Query: 1   MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNRVLPRQAIRASASSSGPST 60
           MASLT SGLLKTPL  SQ+ AN+ QYPP A+GQHQ+PVSL R  P +A+RAS S+SGPST
Sbjct: 1   MASLTTSGLLKTPLKNSQSPANYFQYPPFAYGQHQLPVSLKRAFPGRAVRASPSNSGPST 60

Query: 61  SSSPGLYSAQKFELTTANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS 120
           SSSPGLYSAQKFELT  NVDLVLEDVRPYLIADGGNVDVVSVEDG+VSLKLVGACGSCPS
Sbjct: 61  SSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKE T EAVN+HLDILRPAIRNYGGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNNHLDILRPAIRNYGGSVEV 180

Query: 181 ISIDGGNCVVKYEGPESIGTGIKAAIKEKFPDITNVVFSS 221
           ISI+GG+C+VKYEGPESIGTG+KAAIKE+FPDITNVVFSS
Sbjct: 181 ISINGGDCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS 220

[Close]

BLAST of Carg07106 vs. TAIR 10
Match: AT4G01940.1 (NFU domain protein 1 )

HSP 1 Score: 251.9 bits (642), Expect = 4.7e-67
Identity = 134/195 (68.72%), Postives = 156/195 (80.00%), Query Frame = 0


Query: 31  FGQHQIPVSLNRVLPRQAIRASASSSGPSTSS-----SPGLYSAQKFELTTANVDLVLED 90
           F   +  +S   +  R AI  S SS G   S      S GLYSAQ F+LT  NVDLVLED
Sbjct: 39  FANKRRHISRTAIFHRSAI--SGSSQGEKISPLASGVSSGLYSAQTFDLTPQNVDLVLED 98

Query: 91  VRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPSSTTTMKMGIERVLKEKFGDSVKEIC 150
           VRP+LI+DGGNVDVVSVEDG+VSLKL GAC SCPSS+TTM MGIERVLKEKFGD++K+I 
Sbjct: 99  VRPFLISDGGNVDVVSVEDGVVSLKLQGACTSCPSSSTTMTMGIERVLKEKFGDALKDIR 158

Query: 151 QVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEVISIDGGNCVVKYEGPESIGTGIKAA 210
           QV+DEE K+IT EAVN+HLDILRPAI+NYGGSVEV+S++G +CVVKY GPESIG GI+AA
Sbjct: 159 QVFDEEVKQITVEAVNAHLDILRPAIKNYGGSVEVLSVEGEDCVVKYVGPESIGMGIQAA 218

Query: 211 IKEKFPDITNVVFSS 221
           IKEKF DI+NV F+S
Sbjct: 219 IKEKFKDISNVTFTS 231

[Close]

BLAST of Carg07106 vs. TAIR 10
Match: AT5G49940.1 (NIFU-like protein 2 )

HSP 1 Score: 102.1 bits (253), Expect = 6.0e-22
Identity = 58/148 (39.19%), Postives = 86/148 (58.11%), Query Frame = 0


Query: 74  LTTANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPSSTTTMKMGIERVL 133
           LT  NV+ VL+++RPYL++DGGNV +  ++  IV +KL GACGSCPSST TMKMGIER L
Sbjct: 85  LTEENVESVLDEIRPYLMSDGGNVALHEIDGNIVRVKLQGACGSCPSSTMTMKMGIERRL 144

Query: 134 KEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRP-AIRNYGGSVEVISIDGGNCVVKY 193
            EK  + V       +E   E+  E +   L+ +RP  I    GS++++ I+     ++ 
Sbjct: 145 MEKIPEIVAVEALPDEETGLELNEENIEKVLEEIRPYLIGTADGSLDLVEIEDPIVKIRI 204

Query: 194 EGPE----SIGTGIKAAIKEKFPDITNV 217
            GP     ++   +   ++EK P I  V
Sbjct: 205 TGPAAGVMTVRVAVTQKLREKIPSIAAV 232

[Close]

BLAST of Carg07106 vs. TAIR 10
Match: AT4G25910.1 (NFU domain protein 3 )

HSP 1 Score: 94.0 bits (232), Expect = 1.6e-19
Identity = 56/139 (40.29%), Postives = 84/139 (60.43%), Query Frame = 0


Query: 74  LTTANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPSSTTTMKMGIERVL 133
           LT  NV+ VL++VRP L+ADGGNV +  ++  +V LKL GACGSCPSS+ T+KMGIE  L
Sbjct: 84  LTEENVERVLDEVRPSLMADGGNVALHEIDGLVVVLKLQGACGSCPSSSMTLKMGIESRL 143

Query: 134 KEKFGDSVKEICQVYDEEP--KEITTEAVNSHLDILRPAIRNY-GGSVEVISIDGGNCVV 193
           ++K  + +  + Q  + E    E+  E +   L  LRP +    GG +E++ IDG    V
Sbjct: 144 RDKIPE-IMSVEQFLESETGGLELNDENIEKVLSELRPYLSGTGGGGLELVEIDGYVVKV 203

Query: 194 KYEGPESIGTGIKAAIKEK 210
           +  GP +    ++ A+ +K
Sbjct: 204 RLTGPAAGVMTVRVALTQK 221

[Close]

BLAST of Carg07106 vs. TAIR 10
Match: AT5G49940.2 (NIFU-like protein 2 )

HSP 1 Score: 84.0 bits (206), Expect = 1.7e-16
Identity = 44/87 (50.57%), Postives = 58/87 (66.67%), Query Frame = 0


Query: 74  LTTANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPSSTTTMKMGIERVL 133
           LT  NV+ VL+++RPYL++DGGNV +  ++  IV +KL GACGSCPSST TMKMGIER L
Sbjct: 85  LTEENVESVLDEIRPYLMSDGGNVALHEIDGNIVRVKLQGACGSCPSSTMTMKMGIERRL 144

Query: 134 KEKFGDSVKEICQVYDEEPKEITTEAV 161
            EK  + V       +E   E+  E +
Sbjct: 145 MEKIPEIVAVEALPDEETGLELNEENI 171

[Close]

BLAST of Carg07106 vs. TAIR 10
Match: AT3G20970.1 (NFU domain protein 4 )

HSP 1 Score: 55.1 bits (131), Expect = 8.4e-08
Identity = 34/85 (40.00%), Postives = 50/85 (58.82%), Query Frame = 0


Query: 75  TTANVDLVLED-VRPYLIADGGNVDVVSV--EDGIVSLKLVGACGSCPSSTTTMKMGIER 134
           T A +  +LE  +RP +  DGG+++      E GIV L++ GAC  CPSS+ T+K GIE 
Sbjct: 192 TVAMIKELLETRIRPAVQDDGGDIEYCGFDPESGIVKLRMQGACSGCPSSSVTLKSGIEN 251

Query: 135 VLKEKFGDSVKEICQVYDEEPKEIT 157
           +L   +   VK + Q +D E +E T
Sbjct: 252 MLMH-YVSEVKGVEQEFDGEDEEGT 275

The following BLAST results are available for this feature:

BLAST of Carg07106 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022953177.1	1.8e-116	100.00	nifU-like protein 1, chloroplastic [Cucurbita moschata] >KAG6575747.1 NifU-like ...	[more]
XP_022991837.1	6.3e-114	97.73	nifU-like protein 1, chloroplastic [Cucurbita maxima]	[more]
XP_023547622.1	1.1e-113	97.73	nifU-like protein 1, chloroplastic [Cucurbita pepo subsp. pepo]	[more]
XP_022144330.1	9.4e-102	89.04	nifU-like protein 1, chloroplastic [Momordica charantia]	[more]
XP_008451442.1	1.6e-101	87.27	PREDICTED: nifU-like protein 1, chloroplastic [Cucumis melo]	[more]

BLAST of Carg07106 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93W77	6.6e-66	68.72	NifU-like protein 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NIFU1 PE=1...	[more]
Q93W20	8.5e-21	39.19	NifU-like protein 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NIFU2 PE=1...	[more]
Q84LK7	2.7e-19	40.79	NifU-like protein 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=N...	[more]
Q84RQ7	2.3e-18	40.29	NifU-like protein 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NIFU3 PE=2...	[more]
O32119	5.0e-13	54.29	Putative nitrogen fixation protein YutI OS=Bacillus subtilis (strain 168) OX=224...	[more]

BLAST of Carg07106 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GMI1	8.5e-117	100.00	nifU-like protein 1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111455795...	[more]
A0A6J1JN01	3.0e-114	97.73	nifU-like protein 1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111488364 P...	[more]
A0A6J1CRB9	4.6e-102	89.04	nifU-like protein 1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC11101403...	[more]
A0A1S3BQX1	7.8e-102	87.27	nifU-like protein 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103492737 PE=3 ...	[more]
A0A5A7TT28	3.0e-101	86.82	NifU-like protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G...	[more]

BLAST of Carg07106 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G01940.1	4.7e-67	68.72	NFU domain protein 1	[more]
AT5G49940.1	6.0e-22	39.19	NIFU-like protein 2	[more]
AT4G25910.1	1.6e-19	40.29	NFU domain protein 3	[more]
AT5G49940.2	1.7e-16	50.57	NIFU-like protein 2	[more]
AT3G20970.1	8.4e-08	40.00	NFU domain protein 4	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001075	NIF system FeS cluster assembly, NifU, C-terminal	PFAM	PF01106	NifU	coord: 162..217 e-value: 3.8E-5 score: 23.8 coord: 80..139 e-value: 1.2E-22 score: 79.8
IPR034904	Fe-S cluster assembly domain superfamily	GENE3D	3.30.300.130		coord: 155..218 e-value: 1.3E-11 score: 46.5
IPR034904	Fe-S cluster assembly domain superfamily	GENE3D	3.30.300.130		coord: 71..154 e-value: 4.4E-27 score: 95.9
IPR034904	Fe-S cluster assembly domain superfamily	SUPERFAMILY	117916	Fe-S cluster assembly (FSCA) domain-like	coord: 157..217
IPR034904	Fe-S cluster assembly domain superfamily	SUPERFAMILY	117916	Fe-S cluster assembly (FSCA) domain-like	coord: 77..149
None	No IPR available	PANTHER	PTHR11178	IRON-SULFUR CLUSTER SCAFFOLD PROTEIN NFU-RELATED	coord: 32..219
None	No IPR available	PANTHER	PTHR11178:SF15	NIFU-LIKE PROTEIN 1, CHLOROPLASTIC	coord: 32..219

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg07106-RA	Carg07106-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016226	iron-sulfur cluster assembly
biological_process	GO:0097428	protein maturation by iron-sulfur cluster transfer
cellular_component	GO:0009507	chloroplast
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0051539	4 iron, 4 sulfur cluster binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0051536	iron-sulfur cluster binding