Carg07072 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg07072
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	methyltransferase-like protein 13
Location	Carg_Chr17: 7662608 .. 7664774 (-)
RNA-Seq Expression	Carg07072
Synteny	Carg07072

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTCTCTCTCTCTATAACCCAACGAAAGGTTTTTGTACCAGTTCACCGGAACCACCTAACAACCGCCGCCGGCGTATTCAAGCTCTCATCGTCTTCTTGCCGCTGTGAAATTTCCCGGCGGAGGCGGCCGTAGGTGCAGAGATGAACAGGGACGCGTCGAGCTGCAACACCTACGACTACGGCGATGCTGTCTACTGGGACGCTCGTTACCAGCAGGAGGCTGATTCTTTTGATTGGTACCAGCGTTATGCTGCTCTTCGTCCCTTCGTTCGTAAGTTCATCAGCACTTCCGCCTCCGTACTTATGGTCGGCTGTGGTAATGCTGGTAACTCCCTTCTCCTTTCTGCCATTTTCCAGTTTCATTTTCTTTTGAGATTAGAATAGTTCTCGTGGAGGTATTGGAAGTTGAAATCGGAACGGATCTTCGATGCATTCAAATTTTGGTGGATTTTAAGTTCATCACATTGAGCGATTTAGGCGGTTTAGCGTACGAGAAAATTGAATCTCGGAGCTATCTTGGAATTTTGCTACTGAGAATTCATAGTCAGGAATGAACTTGAACACTTGGGTTTAGTATTCAATTGTTTTGGTGTTAATCCGGTCCGATTCTCACGGAAATTGTCTCCGAGGGAATCATTCTTGAACTGAAGTAGCTTAGTGTTTGGATAAATCAACTTATTTGAGAGATGAAAGCATGAACAGCGTCATATTTTGTTTGATGATTAATCTGCAGATTGGAAACGATACCGTGAAGAAATTGACAACTGATGCAGTGTAATAAAGAGCTTTGGCACATTAAATATGACCCAATTGTTGTCTGCTTCTTCATAAAACATTGATTTTAACCGTTGCATCTTTTGTACTTGTAGTCATGTCAGAGGATATGATCAATGATGGATATGAAGACATAATGAATGTTGACATTTCTTCGGTGGCAGTCGACATAATGAGAAGAAAACATCAGTTCATCCCCCAGTTGAAATGTATCCTCTGAAGCTTGCAACTTAGTTAAATTATCATAAATGGTTTATTTTGATTTCAGAATAGCCCTCAAGTTGTTTATGTTGACAGAATTCTTCTTGCAGCTTAGTTAAATTACCATAAATGGTTTGTTTTGATTTCAGAGTAGCCCTCAAGTTGTTTGTGTTGACAGAATTCTTATTATGGCCATTATTATTGATAAGTTTGTTGAATTGAATCATAAATACTCGGGTATGGTTAAAGTGTAGGAGAGACGGTCCTCTCAAATGGTTGTCGTGGTCGTTGTATTCCCGTTTTTTAGCTAAATTATGTTTTTCTCCTACGAGTTCCCTTGACAAATCTGTATAGACATGGAAATGGATGTCCGAGACATGAGCTTTTTCCCAGACGAGAAATTCGATGCTGTCGTTGATAAAGGTATGAGTTCTTCTTAATCTTCATTTTACACACCATCAAAGTTCCCAGTCTTTAATACTGAAGTTTGCTCTATGTGTTGATCTTTCAATGCCTCGTGGCCTCGTGGTTCTTGATATAGGGACACTTGATGCATTGATGGTATGTGAGCTTTCCATATTTCTAATGCATGTTATTCACTATTTTTTCCCGCGTTCTAATTCGTTAATTGCGTGTACATAGTGTGGCACTGATGCTTCAATTAGTGCAGCTAAAATGCTGGGAGAAGTGAGTAGGTTGTGGACTTTATGAACTTCATAACATCGATCGACTAGCGTAGATTCTGGTCTTTCGGTTTTACATTTCTCCACTAATTTTTGGCTGTTAATTCTATAGGCTTCTTAAACCTGGAGGAGTCTATTTACTGGTAACCATCTTTTATCTCTCATGAGGCTTTGCTTTTTAATGTTCTGTTTGCAGTAAAATAATCAAAATTGGTCGTTTTCAGATCACGTATGGGGACCCAAAAGCTAGGATGCCCTATTTGATGCGACCTTCGTACAATTGGAAGATAGCGTTGTTTATAATCCGTGAGTTCTTTATCATCCTCTCACCGAACTGATCCTTCTTATTTTCGGGTCTCTAATTGATACAGGTCTTCTTTGGATGTTTATTTGCTTCCTGGTTTTTTTTCAGACCATATCTATGCTGAAAAACAAATTTTTTTTTTTTTGAAACCTCAGTTAACTTCTGAAGATAGAAGTCTAATATTAGATTTACAAGAACTCACTCC

mRNA sequence

TTTCTCTCTCTCTATAACCCAACGAAAGGTTTTTGTACCAGTTCACCGGAACCACCTAACAACCGCCGCCGGCGTATTCAAGCTCTCATCGTCTTCTTGCCGCTGTGAAATTTCCCGGCGGAGGCGGCCGTAGGTGCAGAGATGAACAGGGACGCGTCGAGCTGCAACACCTACGACTACGGCGATGCTGTCTACTGGGACGCTCGTTACCAGCAGGAGGCTGATTCTTTTGATTGGTACCAGCGTTATGCTGCTCTTCGTCCCTTCGTTCGTAAGTTCATCAGCACTTCCGCCTCCGTACTTATGGTCGGCTGTGGTAATGCTGTCATGTCAGAGGATATGATCAATGATGGATATGAAGACATAATGAATGTTGACATTTCTTCGGTGGCAGTCGACATAATGAGAAGAAAACATCAGTTCATCCCCCAGTTGAAATACATGGAAATGGATGTCCGAGACATGAGCTTTTTCCCAGACGAGAAATTCGATGCTGTCGTTGATAAAGGGACACTTGATGCATTGATGTGTGGCACTGATGCTTCAATTAGTGCAGCTAAAATGCTGGGAGAAGTGAGTAGGCTTCTTAAACCTGGAGGAGTCTATTTACTGATCACGTATGGGGACCCAAAAGCTAGGATGCCCTATTTGATGCGACCTTCGTACAATTGGAAGATAGCGTTGTTTATAATCCGTGAGTTCTTTATCATCCTCTCACCGAACTGATCCTTCTTATTTTCGGGTCTCTAATTGATACAGGTCTTCTTTGGATGTTTATTTGCTTCCTGGTTTTTTTTCAGACCATATCTATGCTGAAAAACAAATTTTTTTTTTTTTGAAACCTCAGTTAACTTCTGAAGATAGAAGTCTAATATTAGATTTACAAGAACTCACTCC

Coding sequence (CDS)

ATGAACAGGGACGCGTCGAGCTGCAACACCTACGACTACGGCGATGCTGTCTACTGGGACGCTCGTTACCAGCAGGAGGCTGATTCTTTTGATTGGTACCAGCGTTATGCTGCTCTTCGTCCCTTCGTTCGTAAGTTCATCAGCACTTCCGCCTCCGTACTTATGGTCGGCTGTGGTAATGCTGTCATGTCAGAGGATATGATCAATGATGGATATGAAGACATAATGAATGTTGACATTTCTTCGGTGGCAGTCGACATAATGAGAAGAAAACATCAGTTCATCCCCCAGTTGAAATACATGGAAATGGATGTCCGAGACATGAGCTTTTTCCCAGACGAGAAATTCGATGCTGTCGTTGATAAAGGGACACTTGATGCATTGATGTGTGGCACTGATGCTTCAATTAGTGCAGCTAAAATGCTGGGAGAAGTGAGTAGGCTTCTTAAACCTGGAGGAGTCTATTTACTGATCACGTATGGGGACCCAAAAGCTAGGATGCCCTATTTGATGCGACCTTCGTACAATTGGAAGATAGCGTTGTTTATAATCCGTGAGTTCTTTATCATCCTCTCACCGAACTGA

Protein sequence

MNRDASSCNTYDYGDAVYWDARYQQEADSFDWYQRYAALRPFVRKFISTSASVLMVGCGNAVMSEDMINDGYEDIMNVDISSVAVDIMRRKHQFIPQLKYMEMDVRDMSFFPDEKFDAVVDKGTLDALMCGTDASISAAKMLGEVSRLLKPGGVYLLITYGDPKARMPYLMRPSYNWKIALFIIREFFIILSPN

Homology

[Close]

BLAST of Carg07072 vs. NCBI nr
Match: KAG7014266.1 (Methyltransferase-like protein 13 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 394.4 bits (1012), Expect = 5.5e-106
Identity = 194/194 (100.00%), Postives = 194/194 (100.00%), Query Frame = 0


Query: 1   MNRDASSCNTYDYGDAVYWDARYQQEADSFDWYQRYAALRPFVRKFISTSASVLMVGCGN 60
           MNRDASSCNTYDYGDAVYWDARYQQEADSFDWYQRYAALRPFVRKFISTSASVLMVGCGN
Sbjct: 1   MNRDASSCNTYDYGDAVYWDARYQQEADSFDWYQRYAALRPFVRKFISTSASVLMVGCGN 60

Query: 61  AVMSEDMINDGYEDIMNVDISSVAVDIMRRKHQFIPQLKYMEMDVRDMSFFPDEKFDAVV 120
           AVMSEDMINDGYEDIMNVDISSVAVDIMRRKHQFIPQLKYMEMDVRDMSFFPDEKFDAVV
Sbjct: 61  AVMSEDMINDGYEDIMNVDISSVAVDIMRRKHQFIPQLKYMEMDVRDMSFFPDEKFDAVV 120

Query: 121 DKGTLDALMCGTDASISAAKMLGEVSRLLKPGGVYLLITYGDPKARMPYLMRPSYNWKIA 180
           DKGTLDALMCGTDASISAAKMLGEVSRLLKPGGVYLLITYGDPKARMPYLMRPSYNWKIA
Sbjct: 121 DKGTLDALMCGTDASISAAKMLGEVSRLLKPGGVYLLITYGDPKARMPYLMRPSYNWKIA 180

Query: 181 LFIIREFFIILSPN 195
           LFIIREFFIILSPN
Sbjct: 181 LFIIREFFIILSPN 194

[Close]

BLAST of Carg07072 vs. NCBI nr
Match: KAG6575711.1 (EEF1A lysine methyltransferase 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 375.2 bits (962), Expect = 3.5e-100
Identity = 184/184 (100.00%), Postives = 184/184 (100.00%), Query Frame = 0


Query: 1   MNRDASSCNTYDYGDAVYWDARYQQEADSFDWYQRYAALRPFVRKFISTSASVLMVGCGN 60
           MNRDASSCNTYDYGDAVYWDARYQQEADSFDWYQRYAALRPFVRKFISTSASVLMVGCGN
Sbjct: 1   MNRDASSCNTYDYGDAVYWDARYQQEADSFDWYQRYAALRPFVRKFISTSASVLMVGCGN 60

Query: 61  AVMSEDMINDGYEDIMNVDISSVAVDIMRRKHQFIPQLKYMEMDVRDMSFFPDEKFDAVV 120
           AVMSEDMINDGYEDIMNVDISSVAVDIMRRKHQFIPQLKYMEMDVRDMSFFPDEKFDAVV
Sbjct: 61  AVMSEDMINDGYEDIMNVDISSVAVDIMRRKHQFIPQLKYMEMDVRDMSFFPDEKFDAVV 120

Query: 121 DKGTLDALMCGTDASISAAKMLGEVSRLLKPGGVYLLITYGDPKARMPYLMRPSYNWKIA 180
           DKGTLDALMCGTDASISAAKMLGEVSRLLKPGGVYLLITYGDPKARMPYLMRPSYNWKIA
Sbjct: 121 DKGTLDALMCGTDASISAAKMLGEVSRLLKPGGVYLLITYGDPKARMPYLMRPSYNWKIA 180

Query: 181 LFII 185
           LFII
Sbjct: 181 LFII 184

[Close]

BLAST of Carg07072 vs. NCBI nr
Match: XP_022953338.1 (methyltransferase-like protein 13 [Cucurbita moschata])

HSP 1 Score: 374.8 bits (961), Expect = 4.5e-100
Identity = 183/184 (99.46%), Postives = 184/184 (100.00%), Query Frame = 0


Query: 1   MNRDASSCNTYDYGDAVYWDARYQQEADSFDWYQRYAALRPFVRKFISTSASVLMVGCGN 60
           MNRDASSCNTYDYGDAVYWDARYQQEADSFDWYQRYAALRPFVRKFISTSASVLMVGCGN
Sbjct: 1   MNRDASSCNTYDYGDAVYWDARYQQEADSFDWYQRYAALRPFVRKFISTSASVLMVGCGN 60

Query: 61  AVMSEDMINDGYEDIMNVDISSVAVDIMRRKHQFIPQLKYMEMDVRDMSFFPDEKFDAVV 120
           AVMSEDM+NDGYEDIMNVDISSVAVDIMRRKHQFIPQLKYMEMDVRDMSFFPDEKFDAVV
Sbjct: 61  AVMSEDMVNDGYEDIMNVDISSVAVDIMRRKHQFIPQLKYMEMDVRDMSFFPDEKFDAVV 120

Query: 121 DKGTLDALMCGTDASISAAKMLGEVSRLLKPGGVYLLITYGDPKARMPYLMRPSYNWKIA 180
           DKGTLDALMCGTDASISAAKMLGEVSRLLKPGGVYLLITYGDPKARMPYLMRPSYNWKIA
Sbjct: 121 DKGTLDALMCGTDASISAAKMLGEVSRLLKPGGVYLLITYGDPKARMPYLMRPSYNWKIA 180

Query: 181 LFII 185
           LFII
Sbjct: 181 LFII 184

[Close]

BLAST of Carg07072 vs. NCBI nr
Match: XP_023548787.1 (methyltransferase-like protein 13 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 370.5 bits (950), Expect = 8.6e-99
Identity = 180/184 (97.83%), Postives = 183/184 (99.46%), Query Frame = 0


Query: 1   MNRDASSCNTYDYGDAVYWDARYQQEADSFDWYQRYAALRPFVRKFISTSASVLMVGCGN 60
           MNRDASSCNTYDYGDAVYWD+RYQQEADSFDWYQRYAALRPFVRKFISTSASVLMVGCGN
Sbjct: 1   MNRDASSCNTYDYGDAVYWDSRYQQEADSFDWYQRYAALRPFVRKFISTSASVLMVGCGN 60

Query: 61  AVMSEDMINDGYEDIMNVDISSVAVDIMRRKHQFIPQLKYMEMDVRDMSFFPDEKFDAVV 120
           AVMSEDM+NDGYEDIMNVDISSVAVDIMRRKHQFIPQLKYMEMDVRDMSFFPDEKFDAVV
Sbjct: 61  AVMSEDMVNDGYEDIMNVDISSVAVDIMRRKHQFIPQLKYMEMDVRDMSFFPDEKFDAVV 120

Query: 121 DKGTLDALMCGTDASISAAKMLGEVSRLLKPGGVYLLITYGDPKARMPYLMRPSYNWKIA 180
           DKGTLDALMCGTDASISA +MLGEVSRLLKPGGVYLLITYGDPKARMPYLMRPSYNWKIA
Sbjct: 121 DKGTLDALMCGTDASISATQMLGEVSRLLKPGGVYLLITYGDPKARMPYLMRPSYNWKIA 180

Query: 181 LFII 185
           LFII
Sbjct: 181 LFII 184

[Close]

BLAST of Carg07072 vs. NCBI nr
Match: XP_022991924.1 (methyltransferase-like protein 13 [Cucurbita maxima])

HSP 1 Score: 369.0 bits (946), Expect = 2.5e-98
Identity = 180/184 (97.83%), Postives = 183/184 (99.46%), Query Frame = 0


Query: 1   MNRDASSCNTYDYGDAVYWDARYQQEADSFDWYQRYAALRPFVRKFISTSASVLMVGCGN 60
           MNRDASSCNTYDYGDAVYWDARYQQEADSFDWYQRYAALRPFVRKFIST+ASVLMVGCGN
Sbjct: 1   MNRDASSCNTYDYGDAVYWDARYQQEADSFDWYQRYAALRPFVRKFISTTASVLMVGCGN 60

Query: 61  AVMSEDMINDGYEDIMNVDISSVAVDIMRRKHQFIPQLKYMEMDVRDMSFFPDEKFDAVV 120
           AVMSEDM+NDGYEDIMNVDISSVAVDIMRRKHQFIPQLKYMEMDVRDMSFFPDEKFDAVV
Sbjct: 61  AVMSEDMVNDGYEDIMNVDISSVAVDIMRRKHQFIPQLKYMEMDVRDMSFFPDEKFDAVV 120

Query: 121 DKGTLDALMCGTDASISAAKMLGEVSRLLKPGGVYLLITYGDPKARMPYLMRPSYNWKIA 180
           DKGTLDALMCGTDASISAA+MLGEVSRLLK GGVYLLITYGDPKARMPYLMRPSYNWKIA
Sbjct: 121 DKGTLDALMCGTDASISAAQMLGEVSRLLKSGGVYLLITYGDPKARMPYLMRPSYNWKIA 180

Query: 181 LFII 185
           LFII
Sbjct: 181 LFII 184

[Close]

BLAST of Carg07072 vs. ExPASy Swiss-Prot
Match: P0DPD7 (EEF1A lysine methyltransferase 4 OS=Homo sapiens OX=9606 GN=EEF1AKMT4 PE=1 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 2.6e-26
Identity = 62/177 (35.03%), Postives = 102/177 (57.63%), Query Frame = 0


Query: 13  YGDAVYWDARYQQEADS--FDWYQRYAALRPFVRKFISTSASVLMVGCGNAVMSEDMIND 72
           Y +  YWD RYQ  ADS  +DW+  +++ R  +   +     +L++GCGN+ +S ++   
Sbjct: 20  YREVEYWDQRYQGAADSAPYDWFGDFSSFRALLEPELRPEDRILVLGCGNSALSYELFLG 79

Query: 73  GYEDIMNVDISSVAVDIMRRKHQFIPQLKYMEMDVRDMSFFPDEKFDAVVDKGTLDALMC 132
           G+ ++ +VD SSV V  M+ +H  +PQL++  MDVR +  FP   FD V++KGTLDAL+ 
Sbjct: 80  GFPNVTSVDYSSVVVAAMQARHAHVPQLRWETMDVRKLD-FPSASFDVVLEKGTLDALLA 139

Query: 133 G--------TDASISAAKMLGEVSRLLKPGGVYLLITYGDPKARMPYLMRPSYNWKI 180
           G        ++   +  ++L EVSR+L PGG ++ +T   P  R  +  +  Y W +
Sbjct: 140 GERDPWTVSSEGVHTVDQVLSEVSRVLVPGGRFISMTSAAPHFRTRHYAQAYYGWSL 195

[Close]

BLAST of Carg07072 vs. ExPASy Swiss-Prot
Match: P0DPE0 (EEF1A lysine methyltransferase 4 OS=Mus musculus OX=10090 GN=Eef1akmt4 PE=2 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 2.5e-24
Identity = 58/179 (32.40%), Postives = 102/179 (56.98%), Query Frame = 0


Query: 11  YDYGDAVYWDARYQQEADS--FDWYQRYAALRPFVRKFISTSASVLMVGCGNAVMSEDMI 70
           + Y    YWD RY+  ADS  ++W+  +A+ R  +   +     +L++GCGN+ +S ++ 
Sbjct: 18  FQYRQVQYWDQRYKDAADSGPYEWFGDFASFRALLEPELCPEDRILVLGCGNSALSYELF 77

Query: 71  NDGYEDIMNVDISSVAVDIMRRKHQFIPQLKYMEMDVRDMSFFPDEKFDAVVDKGTLDAL 130
             G+ ++ +VD S V V  M+ ++  +P L++  MDVR +  FP   FD V++KGTLDA+
Sbjct: 78  LGGFPNVTSVDYSPVVVAAMQVRYAHVPSLRWETMDVRALD-FPSGSFDVVLEKGTLDAM 137

Query: 131 MCG--------TDASISAAKMLGEVSRLLKPGGVYLLITYGDPKARMPYLMRPSYNWKI 180
           + G        ++   +  ++L EVSRLL PGG ++ +T   P  R+ +  +  Y+W +
Sbjct: 138 LAGEPDPWNVSSEGVHTVDQVLSEVSRLLVPGGRFISMTSAGPHFRIRHYAQSRYDWSL 195

[Close]

BLAST of Carg07072 vs. ExPASy Swiss-Prot
Match: P0DPE1 (EEF1A lysine methyltransferase 4 OS=Bos taurus OX=9913 GN=EEF1AKMT4 PE=2 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 3.2e-24
Identity = 60/177 (33.90%), Postives = 99/177 (55.93%), Query Frame = 0


Query: 13  YGDAVYWDARYQQEADS--FDWYQRYAALRPFVRKFISTSASVLMVGCGNAVMSEDMIND 72
           Y +  YWD RYQ  ADS  ++W+  ++  R  +   +     +L++GCGN+ +S ++   
Sbjct: 20  YREVQYWDQRYQGAADSAPYEWFGDFSCFRDLLEPELRPLDRILVLGCGNSALSYEIFLG 79

Query: 73  GYEDIMNVDISSVAVDIMRRKHQFIPQLKYMEMDVRDMSFFPDEKFDAVVDKGTLDALMC 132
           G+ D+ +VD SSV V  MR ++  +P L++  MDVR +  FP   FD V++KGTLDAL+ 
Sbjct: 80  GFPDVTSVDYSSVVVAAMRARYAHVPTLRWETMDVRALG-FPSGSFDVVLEKGTLDALLT 139

Query: 133 G--------TDASISAAKMLGEVSRLLKPGGVYLLITYGDPKARMPYLMRPSYNWKI 180
           G        ++   +  ++L EVSR+L P G ++ +T   P  R  +  +  Y W +
Sbjct: 140 GEQDPWTVSSEGVHTVDQVLNEVSRVLVPAGRFISLTSAAPHFRTRHYAQAHYGWSL 195

[Close]

BLAST of Carg07072 vs. ExPASy Swiss-Prot
Match: A5PK19 (eEF1A lysine and N-terminal methyltransferase OS=Bos taurus OX=9913 GN=EEF1AKNMT PE=2 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 1.0e-22
Identity = 59/152 (38.82%), Postives = 91/152 (59.87%), Query Frame = 0


Query: 12  DYGDAVYWDARYQQEA-DSFDWYQRYAALRPFVRKFISTSASVLMVGCGNAVMSEDMIND 71
           ++G   YW+  +QQ    +F+WY  Y  L   + K+I     VL+VGCGN+ +SE + + 
Sbjct: 10  EFGSVDYWEKFFQQRGKKAFEWYGTYLELCGVLHKYIKPREKVLVVGCGNSELSEQLYDV 69

Query: 72  GYEDIMNVDISSVAVDIMR-RKHQFIPQLKYMEMDVRDMSFFPDEKFDAVVDKGTLDALM 131
           GY+DI+N+DIS V +  M+ R     P++ +++MD+  M  FPD  F  V+DKGTLDA++
Sbjct: 70  GYQDIVNIDISEVVIKQMKERNASRRPRMSFLKMDMTQME-FPDASFQVVLDKGTLDAVL 129

Query: 132 CGTDASI--SAAKMLGEVSRLLKPGGVYLLIT 160
              +        +ML EV R+L+ GG YL I+
Sbjct: 130 TDEEEKTLQQVDRMLAEVGRVLQVGGRYLCIS 160

[Close]

BLAST of Carg07072 vs. ExPASy Swiss-Prot
Match: Q91YR5 (eEF1A lysine and N-terminal methyltransferase OS=Mus musculus OX=10090 GN=EEF1AKNMT PE=1 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 1.8e-22
Identity = 58/152 (38.16%), Postives = 91/152 (59.87%), Query Frame = 0


Query: 12  DYGDAVYWDARYQQEA-DSFDWYQRYAALRPFVRKFISTSASVLMVGCGNAVMSEDMIND 71
           ++G A YW+  +QQ    +F+WY  Y  L   + K+I     VL++GCGN+ +SE + + 
Sbjct: 10  EFGSADYWEKFFQQRGKTAFEWYGTYLELCEVLHKYIKPKEKVLVIGCGNSELSEQLYDV 69

Query: 72  GYEDIMNVDISSVAVDIMR-RKHQFIPQLKYMEMDVRDMSFFPDEKFDAVVDKGTLDALM 131
           GY+DI+N+DIS V +  M+ R     P + +++MD+  +  FPD  F  V+DKGTLDA++
Sbjct: 70  GYQDIVNIDISEVVIKQMKERNGSRRPHMSFLKMDMTQLE-FPDATFQVVLDKGTLDAVL 129

Query: 132 CGTDASI--SAAKMLGEVSRLLKPGGVYLLIT 160
              +        +ML EV R+L+ GG YL I+
Sbjct: 130 TDEEEVTLRQVDRMLAEVGRVLQVGGRYLCIS 160

[Close]

BLAST of Carg07072 vs. ExPASy TrEMBL
Match: A0A6J1GN36 (methyltransferase-like protein 13 OS=Cucurbita moschata OX=3662 GN=LOC111455920 PE=4 SV=1)

HSP 1 Score: 374.8 bits (961), Expect = 2.2e-100
Identity = 183/184 (99.46%), Postives = 184/184 (100.00%), Query Frame = 0


Query: 1   MNRDASSCNTYDYGDAVYWDARYQQEADSFDWYQRYAALRPFVRKFISTSASVLMVGCGN 60
           MNRDASSCNTYDYGDAVYWDARYQQEADSFDWYQRYAALRPFVRKFISTSASVLMVGCGN
Sbjct: 1   MNRDASSCNTYDYGDAVYWDARYQQEADSFDWYQRYAALRPFVRKFISTSASVLMVGCGN 60

Query: 61  AVMSEDMINDGYEDIMNVDISSVAVDIMRRKHQFIPQLKYMEMDVRDMSFFPDEKFDAVV 120
           AVMSEDM+NDGYEDIMNVDISSVAVDIMRRKHQFIPQLKYMEMDVRDMSFFPDEKFDAVV
Sbjct: 61  AVMSEDMVNDGYEDIMNVDISSVAVDIMRRKHQFIPQLKYMEMDVRDMSFFPDEKFDAVV 120

Query: 121 DKGTLDALMCGTDASISAAKMLGEVSRLLKPGGVYLLITYGDPKARMPYLMRPSYNWKIA 180
           DKGTLDALMCGTDASISAAKMLGEVSRLLKPGGVYLLITYGDPKARMPYLMRPSYNWKIA
Sbjct: 121 DKGTLDALMCGTDASISAAKMLGEVSRLLKPGGVYLLITYGDPKARMPYLMRPSYNWKIA 180

Query: 181 LFII 185
           LFII
Sbjct: 181 LFII 184

[Close]

BLAST of Carg07072 vs. ExPASy TrEMBL
Match: A0A6J1JW63 (methyltransferase-like protein 13 OS=Cucurbita maxima OX=3661 GN=LOC111488419 PE=4 SV=1)

HSP 1 Score: 369.0 bits (946), Expect = 1.2e-98
Identity = 180/184 (97.83%), Postives = 183/184 (99.46%), Query Frame = 0


Query: 1   MNRDASSCNTYDYGDAVYWDARYQQEADSFDWYQRYAALRPFVRKFISTSASVLMVGCGN 60
           MNRDASSCNTYDYGDAVYWDARYQQEADSFDWYQRYAALRPFVRKFIST+ASVLMVGCGN
Sbjct: 1   MNRDASSCNTYDYGDAVYWDARYQQEADSFDWYQRYAALRPFVRKFISTTASVLMVGCGN 60

Query: 61  AVMSEDMINDGYEDIMNVDISSVAVDIMRRKHQFIPQLKYMEMDVRDMSFFPDEKFDAVV 120
           AVMSEDM+NDGYEDIMNVDISSVAVDIMRRKHQFIPQLKYMEMDVRDMSFFPDEKFDAVV
Sbjct: 61  AVMSEDMVNDGYEDIMNVDISSVAVDIMRRKHQFIPQLKYMEMDVRDMSFFPDEKFDAVV 120

Query: 121 DKGTLDALMCGTDASISAAKMLGEVSRLLKPGGVYLLITYGDPKARMPYLMRPSYNWKIA 180
           DKGTLDALMCGTDASISAA+MLGEVSRLLK GGVYLLITYGDPKARMPYLMRPSYNWKIA
Sbjct: 121 DKGTLDALMCGTDASISAAQMLGEVSRLLKSGGVYLLITYGDPKARMPYLMRPSYNWKIA 180

Query: 181 LFII 185
           LFII
Sbjct: 181 LFII 184

[Close]

BLAST of Carg07072 vs. ExPASy TrEMBL
Match: A0A6J1KQY8 (methyltransferase-like protein 13 OS=Cucurbita maxima OX=3661 GN=LOC111497867 PE=4 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 1.9e-91
Identity = 167/184 (90.76%), Postives = 175/184 (95.11%), Query Frame = 0


Query: 1   MNRDASSCNTYDYGDAVYWDARYQQEADSFDWYQRYAALRPFVRKFISTSASVLMVGCGN 60
           M RD SSCNTYDYGDAVYWDARY QEA SFDWYQRYAALRPFVRKFISTSASVLMVGCGN
Sbjct: 1   MYRDVSSCNTYDYGDAVYWDARYLQEAGSFDWYQRYAALRPFVRKFISTSASVLMVGCGN 60

Query: 61  AVMSEDMINDGYEDIMNVDISSVAVDIMRRKHQFIPQLKYMEMDVRDMSFFPDEKFDAVV 120
           AVMSEDMI DGYEDIMN+DISSVA+DIMRRK+QFIPQLKYM+MDV+DMSFFPDEKF AVV
Sbjct: 61  AVMSEDMIKDGYEDIMNIDISSVAIDIMRRKYQFIPQLKYMQMDVKDMSFFPDEKFGAVV 120

Query: 121 DKGTLDALMCGTDASISAAKMLGEVSRLLKPGGVYLLITYGDPKARMPYLMRPSYNWKIA 180
           DKGTLD+LMCGTDA ISAA+MLGEVSRLLKPGGVYLLITYGDPK RMP+LMRPSYNWKI 
Sbjct: 121 DKGTLDSLMCGTDAPISAAQMLGEVSRLLKPGGVYLLITYGDPKVRMPHLMRPSYNWKIT 180

Query: 181 LFII 185
           LFII
Sbjct: 181 LFII 184

[Close]

BLAST of Carg07072 vs. ExPASy TrEMBL
Match: A0A6J1H7U3 (methyltransferase-like protein 13 OS=Cucurbita moschata OX=3662 GN=LOC111461233 PE=4 SV=1)

HSP 1 Score: 344.7 bits (883), Expect = 2.4e-91
Identity = 166/184 (90.22%), Postives = 175/184 (95.11%), Query Frame = 0


Query: 1   MNRDASSCNTYDYGDAVYWDARYQQEADSFDWYQRYAALRPFVRKFISTSASVLMVGCGN 60
           M RD SSCNTYDYGDAVYWDARY QEA SFDWYQRYAALRPFVRKFISTSAS+LMVGCGN
Sbjct: 1   MYRDVSSCNTYDYGDAVYWDARYLQEAGSFDWYQRYAALRPFVRKFISTSASILMVGCGN 60

Query: 61  AVMSEDMINDGYEDIMNVDISSVAVDIMRRKHQFIPQLKYMEMDVRDMSFFPDEKFDAVV 120
           AVMSEDMI DGYEDIMN+DISSVA+DIMRRK+QFIPQLKYM+MDV+DMSFFPDEKF AVV
Sbjct: 61  AVMSEDMIKDGYEDIMNIDISSVAIDIMRRKYQFIPQLKYMQMDVKDMSFFPDEKFGAVV 120

Query: 121 DKGTLDALMCGTDASISAAKMLGEVSRLLKPGGVYLLITYGDPKARMPYLMRPSYNWKIA 180
           DKGTLD+LMCGTDA ISAA+MLGEVSRLLKPGGVYLLITYGDPK RMP+LMRPSYNWKI 
Sbjct: 121 DKGTLDSLMCGTDAPISAAQMLGEVSRLLKPGGVYLLITYGDPKVRMPHLMRPSYNWKIT 180

Query: 181 LFII 185
           LFII
Sbjct: 181 LFII 184

[Close]

BLAST of Carg07072 vs. ExPASy TrEMBL
Match: A0A0A0K6H6 (Methyltransf_11 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G420810 PE=4 SV=1)

HSP 1 Score: 342.0 bits (876), Expect = 1.6e-90
Identity = 164/184 (89.13%), Postives = 176/184 (95.65%), Query Frame = 0


Query: 1   MNRDASSCNTYDYGDAVYWDARYQQEADSFDWYQRYAALRPFVRKFISTSASVLMVGCGN 60
           M RD SSCNTY+YGDAVYWDARY QEA SFDWYQRY++LRPFVRKFISTSASVLMVGCGN
Sbjct: 1   MYRDVSSCNTYNYGDAVYWDARYLQEAGSFDWYQRYSSLRPFVRKFISTSASVLMVGCGN 60

Query: 61  AVMSEDMINDGYEDIMNVDISSVAVDIMRRKHQFIPQLKYMEMDVRDMSFFPDEKFDAVV 120
           AVMSEDM+ DGYEDIMNVDISSVA+D+M+RK+QFIPQLKYMEMDVRDMSFFPDEKF AV+
Sbjct: 61  AVMSEDMVKDGYEDIMNVDISSVAIDMMKRKYQFIPQLKYMEMDVRDMSFFPDEKFGAVI 120

Query: 121 DKGTLDALMCGTDASISAAKMLGEVSRLLKPGGVYLLITYGDPKARMPYLMRPSYNWKIA 180
           DKGTLD+LMCGTDA ISAA+MLGEVSRLLKPGGVYLLITYGDPK RMP+LMRPSYNWKIA
Sbjct: 121 DKGTLDSLMCGTDAPISAAQMLGEVSRLLKPGGVYLLITYGDPKVRMPHLMRPSYNWKIA 180

Query: 181 LFII 185
           LFII
Sbjct: 181 LFII 184

[Close]

BLAST of Carg07072 vs. TAIR 10
Match: AT3G60910.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 294.7 bits (753), Expect = 5.6e-80
Identity = 133/184 (72.28%), Postives = 164/184 (89.13%), Query Frame = 0


Query: 1   MNRDASSCNTYDYGDAVYWDARYQQEADSFDWYQRYAALRPFVRKFISTSASVLMVGCGN 60
           M RD SSCNTY+YGDA+YWDARY Q+A SFDWYQ Y++LRPFVR F+STS+ VLMVGCGN
Sbjct: 1   MQRDVSSCNTYNYGDALYWDARYVQDALSFDWYQCYSSLRPFVRSFVSTSSRVLMVGCGN 60

Query: 61  AVMSEDMINDGYEDIMNVDISSVAVDIMRRKHQFIPQLKYMEMDVRDMSFFPDEKFDAVV 120
           ++MSEDM+ DGYEDIMNVDISSVA+++M+ K+  +PQLKYM+MDVRDMS+F D+ FD ++
Sbjct: 61  SLMSEDMVKDGYEDIMNVDISSVAIEMMQTKYASVPQLKYMQMDVRDMSYFEDDSFDTII 120

Query: 121 DKGTLDALMCGTDASISAAKMLGEVSRLLKPGGVYLLITYGDPKARMPYLMRPSYNWKIA 180
           DKGTLD+LMCG+DA +SA++MLGEVSRL+KPGG Y LITYGDPK RMP+L R +YNWKI+
Sbjct: 121 DKGTLDSLMCGSDALLSASRMLGEVSRLIKPGGTYFLITYGDPKVRMPHLTRSAYNWKIS 180

Query: 181 LFII 185
           L+II
Sbjct: 181 LYII 184

[Close]

BLAST of Carg07072 vs. TAIR 10
Match: AT3G17365.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 180.6 bits (457), Expect = 1.2e-45
Identity = 86/181 (47.51%), Postives = 126/181 (69.61%), Query Frame = 0


Query: 5   ASSCNTYDYGDAVYWDARYQQEADSFDWYQRYAALRPFVRKFI-STSASVLMVGCGNAVM 64
           A+   T  Y +  YWD RY+ E++ FDWYQ+Y+ L P +  ++   +  VL++GCGN+  
Sbjct: 2   ATEAPTQSYSEQWYWDDRYKNESEPFDWYQKYSPLAPLINLYVPQRNQRVLVIGCGNSAF 61

Query: 65  SEDMINDGYEDIMNVDISSVAVDIMRRKHQFIPQLKYMEMDVRDMSFFPDEKFDAVVDKG 124
           SE M++DGYED++++DISSV +D M +K+   PQLKY++MDVRDM  F D  FDAV+DKG
Sbjct: 62  SEGMVDDGYEDVVSIDISSVVIDTMIKKYSDRPQLKYLKMDVRDMKAFEDASFDAVIDKG 121

Query: 125 TLDALMCGTDASISAAKMLGEVSRLLKPGGVYLLITYGDPKARMPYLMRPSYNWKIALFI 184
           TLD+++CG+++   + +ML EV R+LK  GVY+LITYG P  R+  L + S +W   L +
Sbjct: 122 TLDSILCGSNSRQYSTQMLEEVWRVLKDKGVYILITYGAPIYRL-RLFKESCSWTTKLHV 181

[Close]

BLAST of Carg07072 vs. TAIR 10
Match: AT3G17365.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 144.4 bits (363), Expect = 9.3e-35
Identity = 84/219 (38.36%), Postives = 118/219 (53.88%), Query Frame = 0


Query: 5   ASSCNTYDYGDAVYWDARYQQEADSFDWYQRYAALRPFVRKFI-STSASVLMVGCGNAVM 64
           A+   T  Y +  YWD RY+ E++ FDWYQ+Y+ L P +  ++   +  VL++GCGN+  
Sbjct: 2   ATEAPTQSYSEQWYWDDRYKNESEPFDWYQKYSPLAPLINLYVPQRNQRVLVIGCGNSAF 61

Query: 65  SEDMINDGYEDIMNVDISSVAVDIMRRKHQFIPQLKYMEMDVRDMSFFPDEKFDAVVDKG 124
           SE M++DGYED++++DISSV +D M +K+   PQLKY++MDVRDM  F D  FDAV+DKG
Sbjct: 62  SEGMVDDGYEDVVSIDISSVVIDTMIKKYSDRPQLKYLKMDVRDMKAFEDASFDAVIDKG 121

Query: 125 T---------------------LDALMC------------GTDASISAAKML-----GEV 184
                                 LD  +C            G     +  K L      E 
Sbjct: 122 VLTTCLNIITSLKCIMFHLYNILDFCVCVFRNLRLHFGDVGVIRGNTQHKCLKRFGVDET 181

[Close]

BLAST of Carg07072 vs. TAIR 10
Match: AT4G34360.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 105.9 bits (263), Expect = 3.7e-23
Identity = 58/177 (32.77%), Postives = 96/177 (54.24%), Query Frame = 0


Query: 13  YGDAVYWDARYQQEADSFDWYQRYAALRPFVRKFISTSASVLMVGCGNAVMSEDMINDGY 72
           Y D  YWD R+  E + ++W++ Y+  +  +   I  S+SVL +GCGN+ + E++  DG 
Sbjct: 15  YLDPHYWDERFSSE-EHYEWFKDYSHFQHLIISNIKPSSSVLELGCGNSQLCEELYKDGI 74

Query: 73  EDIMNVDISSVAVDIMRRK--HQFIPQLKYMEMDVRDMSFFPDEKFDAVVDKGTLDALMC 132
            DI  +D+SSVAV+ M+ +   +   ++K ++ D+ D+  F  E FD V++KGT+D L  
Sbjct: 75  VDITCIDLSSVAVEKMQSRLLPKGYKEIKVVQADMLDLP-FDSESFDVVIEKGTMDVLFV 134

Query: 133 GT--------DASISAAKMLGEVSRLLKPGGVYLLITYGDPKARMPYLMRPSYNWKI 180
                     +        L  V R+LKP G+++ IT+G P  R P    P + W +
Sbjct: 135 DAGDPWNPRPETVSKVMATLDGVHRVLKPDGIFISITFGQPHFRRPLFKDPKFTWSM 189

[Close]

BLAST of Carg07072 vs. TAIR 10
Match: AT2G31740.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 97.4 bits (241), Expect = 1.3e-20
Identity = 64/200 (32.00%), Postives = 106/200 (53.00%), Query Frame = 0


Query: 2   NRDASSCNTY-----DYGDAVYWDARY--QQEADSFDWY----QRYAALRPFVRKFISTS 61
           N+ A+S + +     D+     WD  +  +   DSF+WY    Q + +L P ++   S+S
Sbjct: 7   NKAAASTDDFLQTLEDFTSKENWDKFFTLRGNDDSFEWYAEWPQLHDSLLPLLQDSSSSS 66

Query: 62  A---SVLMVGCGNAVMSEDMINDGYEDIMNVDISSVAV-DIMRRKHQFIPQLKYMEMDVR 121
           +    +L+ GCGN+ ++E + + G+ DI NVD S V + D++RR  +  P+L++  MD+ 
Sbjct: 67  SDSLQILVPGCGNSRLTEHLYDAGFRDITNVDFSKVVISDMLRRNIRTRPELRWRVMDIT 126

Query: 122 DMSFFPDEKFDAVVDKGTLDALMCGTDASISAAKMLGEVSRLLKPGGVYLLITYGDPKAR 181
            M    DE FD V+DKG LDALM     +    + L E  R+LKPGG ++ +T  +    
Sbjct: 127 KMQ-LADESFDTVLDKGALDALMEPEVGTKLGNQYLSEAKRVLKPGGKFICLTLAESHVL 186

Query: 182 MPYLMRPSYNWKIALFIIRE 187
                R  + WK+ +  I +
Sbjct: 187 ALLFSRFRFGWKMNVHSIAQ 205

The following BLAST results are available for this feature:

BLAST of Carg07072 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7014266.1	5.5e-106	100.00	Methyltransferase-like protein 13 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6575711.1	3.5e-100	100.00	EEF1A lysine methyltransferase 4, partial [Cucurbita argyrosperma subsp. sororia...	[more]
XP_022953338.1	4.5e-100	99.46	methyltransferase-like protein 13 [Cucurbita moschata]	[more]
XP_023548787.1	8.6e-99	97.83	methyltransferase-like protein 13 [Cucurbita pepo subsp. pepo]	[more]
XP_022991924.1	2.5e-98	97.83	methyltransferase-like protein 13 [Cucurbita maxima]	[more]

BLAST of Carg07072 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P0DPD7	2.6e-26	35.03	EEF1A lysine methyltransferase 4 OS=Homo sapiens OX=9606 GN=EEF1AKMT4 PE=1 SV=1	[more]
P0DPE0	2.5e-24	32.40	EEF1A lysine methyltransferase 4 OS=Mus musculus OX=10090 GN=Eef1akmt4 PE=2 SV=1	[more]
P0DPE1	3.2e-24	33.90	EEF1A lysine methyltransferase 4 OS=Bos taurus OX=9913 GN=EEF1AKMT4 PE=2 SV=1	[more]
A5PK19	1.0e-22	38.82	eEF1A lysine and N-terminal methyltransferase OS=Bos taurus OX=9913 GN=EEF1AKNMT...	[more]
Q91YR5	1.8e-22	38.16	eEF1A lysine and N-terminal methyltransferase OS=Mus musculus OX=10090 GN=EEF1AK...	[more]

BLAST of Carg07072 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GN36	2.2e-100	99.46	methyltransferase-like protein 13 OS=Cucurbita moschata OX=3662 GN=LOC111455920 ...	[more]
A0A6J1JW63	1.2e-98	97.83	methyltransferase-like protein 13 OS=Cucurbita maxima OX=3661 GN=LOC111488419 PE...	[more]
A0A6J1KQY8	1.9e-91	90.76	methyltransferase-like protein 13 OS=Cucurbita maxima OX=3661 GN=LOC111497867 PE...	[more]
A0A6J1H7U3	2.4e-91	90.22	methyltransferase-like protein 13 OS=Cucurbita moschata OX=3662 GN=LOC111461233 ...	[more]
A0A0A0K6H6	1.6e-90	89.13	Methyltransf_11 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G42...	[more]

BLAST of Carg07072 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G60910.1	5.6e-80	72.28	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT3G17365.1	1.2e-45	47.51	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT3G17365.2	9.3e-35	38.36	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT4G34360.1	3.7e-23	32.77	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT2G31740.1	1.3e-20	32.00	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 10..191 e-value: 4.6E-60 score: 204.4
None	No IPR available	PANTHER	PTHR12176	SAM-DEPENDENT METHYLTRANSFERASE SUPERFAMILY PROTEIN	coord: 9..184
None	No IPR available	PANTHER	PTHR12176:SF66	S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASES SUPERFAMILY PROTEIN	coord: 9..184
None	No IPR available	CDD	cd02440	AdoMet_MTases	coord: 53..160 e-value: 2.06931E-10 score: 53.5879
IPR013216	Methyltransferase type 11	PFAM	PF08241	Methyltransf_11	coord: 54..156 e-value: 6.1E-15 score: 55.6
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 10..166

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg07072-RA	Carg07072-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
molecular_function	GO:0008168	methyltransferase activity