Carg07043 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg07043
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	mitochondrial import inner membrane translocase subunit TIM22-4
Location	Carg_Chr01: 3735550 .. 3740013 (-)
RNA-Seq Expression	Carg07043
Synteny	Carg07043

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTTCTTGCTTTCTCGCTCCTTCTCAATCCTTGAAGCTTTTCTTCTTCCTCTCGTATCAATCTCTTCTCCACATCAATGGCTTCCAGTTCTGGCGATGGATCTTCGCACGATCCCGCGAGCTCGAGCGAGTTGGAGAAGCCGCCATTGGAGCCTATAAGGATGCCTACAGTCGAGGAGATTCGCGGCCAAGATATTTGGAACAACTGTGCAGTTCGCAGTGTTGTTAGTGGAGTCATGGGTACGTTAGCCAATTTCTTTCAGTTGTTTTAACTACAATTTGCTTGTTGCTTTTTGTAAATCATTTCCCGCATTCTCACTCCTGTACTTTATTCTAGTTTCTCTTGCCAATACTGGAATGAATCTTCATGTTTTTTCCTCTTATCGTTTACAACAGTATTACTATCTCTCCGTCTCTTAAATGTGATGCCTTATAGTTTCTGTTAAATTGTTCATATTTTAGTTTGAGTCTGGGCTGTATATTTAGTTGCCTAGAGGATTAAGATTGAGAGGCTCAGCCACCGTGTATATGTTGCTTTAATCATAATTTATGTGCTGAAATATTTGCAGGCGGTGGACTTGGATTGTTCATGGGTTTATTTTTGGGGGCATTGGATAATCCATTAATGCAAGACGAGATGACTGCTAGACAGCAATTTGTTTATACTGCAAAACAAATGGGACGGCGTAGTTGGGGTTCTGCAAAAGCGTTTGCAGTTATGGGCTTTGTTTTCTCAGCAGCAGAATGTGTTGTAGAAAAGGTATCATGATCGTTTATTATTATTTTTTTTCTTCTCAGTTTCTGAATGTGATTTTATGGGATCTAATTCTTTCTTCATGTGCTTAGGCTCGAGCCAAACATGATATAACTAATACAGTAGTCGCTGGTTGTGTAACTGGTGGTTCAATATCAGCTAAAGGTAGGCCCTCTTACCCTTGTATCCATGTACGTTCCTCATTTTCTACTCAAGTGTCACCATGTGAATCCTTCAGCTTCTAGGCATGTAAATGTCTTTCACGAAAAAAATTCAGGGGTTCAGTACTCCTGTTGAGCTAAAATACTGGAGTTCGTGCATTTTAGATTGATTCGTGTTCAGAATGAAATTATAAGTTTATGACCTCGATCTTGCAGTAGGAAATAATGTTCTATAGGCTCTACCTCTGCATCCTTGAATTCTAAGGATAATATTATAGGCTCTTGTTTTCCGGGTCACATTACAAAGAAATTTGCAGGGACAGGGTATACATGTGTGCACAAGAAGTTCAATTGGCCTTGCTAATTTATTTCCTTACTATACTTTCTGACTGATCTTGGTTGAAATTTCATCCTTCAAGTTGCCAGAGAATAACCTGTTCGCTTTCAATAACACTGCTGTGCTAGCTTGTGCCCTTTTTTCTCTCTATCTGTTGTGAACAAAAGCTGCAAAGAGACAAAGGCATTGGTCTCCTAGGGGCATCTTTGGATATTTAAAACTACTTAAACCTGACCTGATATCAAATCTTGAAAGATCCTCCAGCTAAACCTCACATAGAATAACTCTAGAAGCATTTGGCCAAAGTAGCCTACCCATCCTTTTAGATTAAGGAATCACCGAGGAGAGCGTCCTTTGTCGAGAAGGGATTAACCCAGCTTATCCCAGAAACACAGAGTTTCTCCACGACAGCTGAAACGTGAAGAGAGAAGAAAAACATCTAGCATTCTCAATATATTCATGTATAATAAATAGCGATTTGGGAAACAAATGTGCAGATTCCTTTCCTTGTGATGTCCCACATTGGTTGGGGAGGAGAACAAATCACCCTTTATAAGGGTGTGGAAACCTTCCCCTAGTAGACGCGTTTTAAAGCCTTGAGGGGAAGCCCGAAAGGGAAAGCCCAAATAGGACAATATCTGCTAGCGGTGGATCTGGGTCGTTACAAATGGTATCAGAGCCAGTCACCAGACGATGTGCCGGCCTTCTCGTTGTTCCCCGAAGGGGGGTAGACACGAGGCGGTGTGCCAGTAAGGACGCTGGACCCCAAAGGGGGGTGGATTTGGGGGCGGTCCCACATCGATTGGATAAAGGAACGAGTGCCAGCGAGGACGCTGGGCCCCGAAGGGGGGTGGATTGTGATGTCCCACATTGGTCGGGGAGAAGAACAAATCACCCTTTATAAGGGTGTGGAAACCTTCCCCTAGTAGACGCGTTTTAAAGCCTTAAGGGGAAGCCCGAAAGGGAAAGCCCAAAGAGGACAATATCTGCTGGCGGTGGATCTGGGTCGTTACATTCCTGGTCTTCATTCATGTTTAAGCCTTCAAGCATAGTCTAAAGTAAAACTTTTACCTAACATTAGATACCAGTCTTTCCATGAAGCCTTAAACTTATTTTTGGAAAATAACTAGTTGCATTAAGTCTATGACAAAGGGTTTCGCTACAAAATACTTCTCTAGAGTCCTGATTGACCTGTCATTCTCACTGCTAACATTTCAGATTTTCAGCATCTGAAGAATTCTTTCCCATTCCTAGATTTCCTCGTATTTCAAATTTGAATTCAAGTTAAAGCATATGGAACTATAGTAGTGATGTCAGCGCTGCCTTACATTTGGAAATTTGCTACCGGTTAGATCATACAGCACTTGACAGGTATTTGACAGTGGCCAGCCCTTGATTCAAATATCTTTTGAAAATGTAACTTATTTTATTTGCCAACCCGGGAAGATTGGGGGGGGGAGGGGGGGGGGGGGTGTGGAGATCATGTGAGAAGATAGGCCAAACACCCAACAAGAGGAGCCACCTAACAAACATTTACAATTGCCACAAAATATCCAATTGTTGATGATAAGAAAAATAAGGGAATATCTAAATCTATCAAAAGTGAGCTAGTAGAAGCTAAGTAGTTATGGAGTTGTAGCTAGGTTTTCCTTTTAATTTTGTGTGTTTTGGTTAGGTTGGTTTGGCTTTGACTGGAGCTGGTATCCAATTTTTTGTATGCCATTCTTGCACTAGCTTATAGAGCCAAAGAAACGTACATTTTTTGTGTAGAGAAATTATAGGACAGTACAAGGAAAGTTTCAGTTAAGCTTTAGAAGCAATTTCATAAAAATATCTTTAGCATGATTTGTGCACACCGCTTTGGTCAACGGTTAGGCAACGACATACGAGGTTTCCTACTAACTGATATAAAGCATTCTTATATGAAAAGTGATAGAAAACATTCCCAATAGATGGAGATATGCCAAATTATGTGTTTATTCATATAAGAGGTTACCATCCATGCCATCGATTTATTCATGTAAAGAGGAATGCTCCCCTCTTGATTTAAGCCATAGATGGAGATATGCCAAATGTTAAAGTAATCACAACCAGATTCAATAGGATCAAACGGTTATCTTAGGAGAATAGTTGCGAGACGTATGTAAACTGACCTGAACACTTTTGGATATCTAAAAAAATATCTTTTGAGGGATTCCCGTCCAACAACCATAAGTTTTTTCTTGCCTTTTGTCAAAATCTGGATTGTAGTTTCTGCTTTTACTTCACATAGCTATTCTGCATCAAACTTTGGGACAATCTCTTTAGCCTCGTTCCATTCTCGTGTTTAAATGAGCAATGTTTGTTTGAAGAATGAATGATTTACAGGTGGAAAAGGACGGAATAACATTAACGACCCAGATCCATCATTAGCAGATATTGTCCTCTTTGGGCTTTCCCTTTCGGGCTTCCCCTCAAGGCTTTTAACGCATCTGCTAGGGGAAGGTTTCCACACCCTTATAAATTGTAGTTTGTTCTCCTCCCCAACCAATGTGGTGTTAGATGATAAAACTCCCACCTCGGCTAATTTAGGGAATGATCGTGGGTTTATAAACAAAGAATACTCTCTCCATTGGTATGAGGCCTTTTGGGGAAGCCCAAAGCTAAACCATGAGAGCTTATGCTCAAAATGGACAATATCATACCATTGTGGAGAGTCATGTTCGTCTAACATGTGGAACATCACAAACACAAAGGGGCTATGTTAACTGAATTGGTTATTTACCATGCTGATTAGTTTGTTCATTGTTTTTAACATGCTTCCCATATCTCAGCTTGTATCGTCTTAATTCAGGTGGCCCAAAGGCAGCTTGTGCCGGTTGTGCTGGTTTTGCAGCATTTTCAGTCGTGATAGAGAAGTTTTTGGACAGACACACTTGAGCTGCGTCTTGTGCTCACAATTTTCTGGTAGCAAAAAGAAAAAGAATCAATGGTAGAGAAATAATAGGTAGCCCGCATTGTAACCATTCTTTTCATCACATAAATCCGAACCCCCTTTTCTATTTGGGATGTACTTTTTCAATATTTTCCTCATTGTTTTAGTAACATTATTCACAATCCCATTTTGTTGGAACAAATTTGTACTCATAGAGACAGGAAATTGGACCATTTTTGCAGAGCTTTAAGATGTGTCATTCAGGTAGCCTCTGGTCATTACGCATTTCGATTTTTTTT

mRNA sequence

TTTTCTTGCTTTCTCGCTCCTTCTCAATCCTTGAAGCTTTTCTTCTTCCTCTCGTATCAATCTCTTCTCCACATCAATGGCTTCCAGTTCTGGCGATGGATCTTCGCACGATCCCGCGAGCTCGAGCGAGTTGGAGAAGCCGCCATTGGAGCCTATAAGGATGCCTACAGTCGAGGAGATTCGCGGCCAAGATATTTGGAACAACTGTGCAGTTCGCAGTGTTGTTAGTGGAGTCATGGGCGGTGGACTTGGATTGTTCATGGGTTTATTTTTGGGGGCATTGGATAATCCATTAATGCAAGACGAGATGACTGCTAGACAGCAATTTGTTTATACTGCAAAACAAATGGGACGGCGTAGTTGGGGTTCTGCAAAAGCGTTTGCAGTTATGGGCTTTGTTTTCTCAGCAGCAGAATGTGTTGTAGAAAAGTTTCTGAATGCTCGAGCCAAACATGATATAACTAATACAGTAGTCGCTGGTTGTGTAACTGGTGGTTCAATATCAGCTAAAGGTGGCCCAAAGGCAGCTTGTGCCGGTTGTGCTGGTTTTGCAGCATTTTCAGTCGTGATAGAGAAGTTTTTGGACAGACACACTTGAGCTGCGTCTTGTGCTCACAATTTTCTGGTAGCAAAAAGAAAAAGAATCAATGGTAGAGAAATAATAGGTAGCCCGCATTGTAACCATTCTTTTCATCACATAAATCCGAACCCCCTTTTCTATTTGGGATGTACTTTTTCAATATTTTCCTCATTGTTTTAGTAACATTATTCACAATCCCATTTTGTTGGAACAAATTTGTACTCATAGAGACAGGAAATTGGACCATTTTTGCAGAGCTTTAAGATGTGTCATTCAGGTAGCCTCTGGTCATTACGCATTTCGATTTTTTTT

Coding sequence (CDS)

ATGGCTTCCAGTTCTGGCGATGGATCTTCGCACGATCCCGCGAGCTCGAGCGAGTTGGAGAAGCCGCCATTGGAGCCTATAAGGATGCCTACAGTCGAGGAGATTCGCGGCCAAGATATTTGGAACAACTGTGCAGTTCGCAGTGTTGTTAGTGGAGTCATGGGCGGTGGACTTGGATTGTTCATGGGTTTATTTTTGGGGGCATTGGATAATCCATTAATGCAAGACGAGATGACTGCTAGACAGCAATTTGTTTATACTGCAAAACAAATGGGACGGCGTAGTTGGGGTTCTGCAAAAGCGTTTGCAGTTATGGGCTTTGTTTTCTCAGCAGCAGAATGTGTTGTAGAAAAGTTTCTGAATGCTCGAGCCAAACATGATATAACTAATACAGTAGTCGCTGGTTGTGTAACTGGTGGTTCAATATCAGCTAAAGGTGGCCCAAAGGCAGCTTGTGCCGGTTGTGCTGGTTTTGCAGCATTTTCAGTCGTGATAGAGAAGTTTTTGGACAGACACACTTGA

Protein sequence

MASSSGDGSSHDPASSSELEKPPLEPIRMPTVEEIRGQDIWNNCAVRSVVSGVMGGGLGLFMGLFLGALDNPLMQDEMTARQQFVYTAKQMGRRSWGSAKAFAVMGFVFSAAECVVEKFLNARAKHDITNTVVAGCVTGGSISAKGGPKAACAGCAGFAAFSVVIEKFLDRHT

Homology

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BLAST of Carg07043 vs. NCBI nr
Match: KAG7037014.1 (Mitochondrial import inner membrane translocase subunit TIM22-1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 342.0 bits (876), Expect = 2.9e-90
Identity = 173/173 (100.00%), Postives = 173/173 (100.00%), Query Frame = 0


Query: 1   MASSSGDGSSHDPASSSELEKPPLEPIRMPTVEEIRGQDIWNNCAVRSVVSGVMGGGLGL 60
           MASSSGDGSSHDPASSSELEKPPLEPIRMPTVEEIRGQDIWNNCAVRSVVSGVMGGGLGL
Sbjct: 1   MASSSGDGSSHDPASSSELEKPPLEPIRMPTVEEIRGQDIWNNCAVRSVVSGVMGGGLGL 60

Query: 61  FMGLFLGALDNPLMQDEMTARQQFVYTAKQMGRRSWGSAKAFAVMGFVFSAAECVVEKFL 120
           FMGLFLGALDNPLMQDEMTARQQFVYTAKQMGRRSWGSAKAFAVMGFVFSAAECVVEKFL
Sbjct: 61  FMGLFLGALDNPLMQDEMTARQQFVYTAKQMGRRSWGSAKAFAVMGFVFSAAECVVEKFL 120

Query: 121 NARAKHDITNTVVAGCVTGGSISAKGGPKAACAGCAGFAAFSVVIEKFLDRHT 174
           NARAKHDITNTVVAGCVTGGSISAKGGPKAACAGCAGFAAFSVVIEKFLDRHT
Sbjct: 121 NARAKHDITNTVVAGCVTGGSISAKGGPKAACAGCAGFAAFSVVIEKFLDRHT 173

[Close]

BLAST of Carg07043 vs. NCBI nr
Match: XP_022948292.1 (mitochondrial import inner membrane translocase subunit TIM22-4 [Cucurbita moschata] >XP_022998612.1 mitochondrial import inner membrane translocase subunit TIM22-4 [Cucurbita maxima] >XP_023523181.1 mitochondrial import inner membrane translocase subunit TIM22-4 [Cucurbita pepo subsp. pepo] >KAG6607337.1 Mitochondrial import inner membrane translocase subunit TIM22-1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 330.1 bits (845), Expect = 1.1e-86
Identity = 170/173 (98.27%), Postives = 170/173 (98.27%), Query Frame = 0


Query: 1   MASSSGDGSSHDPASSSELEKPPLEPIRMPTVEEIRGQDIWNNCAVRSVVSGVMGGGLGL 60
           MASSSGDGSSHDPASSSELEKPPLEPIRMPTVEEIRGQDIWNNCAVRSVVSGVMGGGLGL
Sbjct: 1   MASSSGDGSSHDPASSSELEKPPLEPIRMPTVEEIRGQDIWNNCAVRSVVSGVMGGGLGL 60

Query: 61  FMGLFLGALDNPLMQDEMTARQQFVYTAKQMGRRSWGSAKAFAVMGFVFSAAECVVEKFL 120
           FMGLFLGALDNPLMQDEMTARQQFVYTAKQMGRRSWGSAKAFAVMGFVFSAAECVVEK  
Sbjct: 61  FMGLFLGALDNPLMQDEMTARQQFVYTAKQMGRRSWGSAKAFAVMGFVFSAAECVVEK-- 120

Query: 121 NARAKHDITNTVVAGCVTGGSISAKGGPKAACAGCAGFAAFSVVIEKFLDRHT 174
            ARAKHDITNTVVAGCVTGGSISAKGGPKAACAGCAGFAAFSVVIEKFLDRHT
Sbjct: 121 -ARAKHDITNTVVAGCVTGGSISAKGGPKAACAGCAGFAAFSVVIEKFLDRHT 170

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BLAST of Carg07043 vs. NCBI nr
Match: XP_022154905.1 (mitochondrial import inner membrane translocase subunit TIM22-4-like [Momordica charantia])

HSP 1 Score: 309.7 bits (792), Expect = 1.6e-80
Identity = 154/173 (89.02%), Postives = 167/173 (96.53%), Query Frame = 0


Query: 1   MASSSGDGSSHDPASSSELEKPPLEPIRMPTVEEIRGQDIWNNCAVRSVVSGVMGGGLGL 60
           MA+++G+GSSHDP SS+E EKPP+EPIRMPTVEEIRGQDIWNNCAVRSVVSGVMGGGLGL
Sbjct: 1   MATNAGNGSSHDPTSSNEAEKPPIEPIRMPTVEEIRGQDIWNNCAVRSVVSGVMGGGLGL 60

Query: 61  FMGLFLGALDNPLMQDEMTARQQFVYTAKQMGRRSWGSAKAFAVMGFVFSAAECVVEKFL 120
           FMGLFLGALDNPLMQ+EMTA+QQF++TAKQMGRRSWGSAKAFAVMGF+FSAAECVVEK  
Sbjct: 61  FMGLFLGALDNPLMQEEMTAKQQFIFTAKQMGRRSWGSAKAFAVMGFIFSAAECVVEK-- 120

Query: 121 NARAKHDITNTVVAGCVTGGSISAKGGPKAACAGCAGFAAFSVVIEKFLDRHT 174
            ARAKHD TNTVVAGCVTGGSISAKGGPKAACAGCAGFAAFSV+IEKFLDRH+
Sbjct: 121 -ARAKHDTTNTVVAGCVTGGSISAKGGPKAACAGCAGFAAFSVLIEKFLDRHS 170

[Close]

BLAST of Carg07043 vs. NCBI nr
Match: XP_038893922.1 (mitochondrial import inner membrane translocase subunit TIM22-4-like [Benincasa hispida])

HSP 1 Score: 304.7 bits (779), Expect = 5.2e-79
Identity = 154/173 (89.02%), Postives = 165/173 (95.38%), Query Frame = 0


Query: 1   MASSSGDGSSHDPASSSELEKPPLEPIRMPTVEEIRGQDIWNNCAVRSVVSGVMGGGLGL 60
           MAS+SGD SS DP +S++ E PP+EPIRMPT+EEIRGQDIWNNCAVRSVVSGVMGGGLGL
Sbjct: 1   MASTSGDRSSLDPPTSNDSENPPIEPIRMPTLEEIRGQDIWNNCAVRSVVSGVMGGGLGL 60

Query: 61  FMGLFLGALDNPLMQDEMTARQQFVYTAKQMGRRSWGSAKAFAVMGFVFSAAECVVEKFL 120
           FMGLFLGALDNPLMQDEMTA+QQFVYTAKQMGRRSWGSAKAFAVMGF+FSAAECVVEK  
Sbjct: 61  FMGLFLGALDNPLMQDEMTAKQQFVYTAKQMGRRSWGSAKAFAVMGFIFSAAECVVEK-- 120

Query: 121 NARAKHDITNTVVAGCVTGGSISAKGGPKAACAGCAGFAAFSVVIEKFLDRHT 174
            ARAKHD+TNTVVAGCVTGGSISAKGGPKAACAGCAGFAAFSV+IEKFLDRH+
Sbjct: 121 -ARAKHDMTNTVVAGCVTGGSISAKGGPKAACAGCAGFAAFSVLIEKFLDRHS 170

[Close]

BLAST of Carg07043 vs. NCBI nr
Match: XP_008457318.1 (PREDICTED: mitochondrial import inner membrane translocase subunit TIM22-4-like [Cucumis melo])

HSP 1 Score: 292.7 bits (748), Expect = 2.0e-75
Identity = 145/173 (83.82%), Postives = 163/173 (94.22%), Query Frame = 0


Query: 1   MASSSGDGSSHDPASSSELEKPPLEPIRMPTVEEIRGQDIWNNCAVRSVVSGVMGGGLGL 60
           MAS+SGDG S DP +S++ E+PP+ P+RMPTVEEIRGQDIW+NCAVRSVVSGVMGGG+GL
Sbjct: 1   MASTSGDGFSPDPPTSNDAEQPPIAPMRMPTVEEIRGQDIWDNCAVRSVVSGVMGGGIGL 60

Query: 61  FMGLFLGALDNPLMQDEMTARQQFVYTAKQMGRRSWGSAKAFAVMGFVFSAAECVVEKFL 120
           F+GLFLG++DN LMQDEMTARQQFVYTAKQMGRR WGSAK+FAVMGF+FSAAECVVEK  
Sbjct: 61  FLGLFLGSMDNSLMQDEMTARQQFVYTAKQMGRRGWGSAKSFAVMGFIFSAAECVVEK-- 120

Query: 121 NARAKHDITNTVVAGCVTGGSISAKGGPKAACAGCAGFAAFSVVIEKFLDRHT 174
            ARAKHD+TNTVVAGCVTGG+ISAKGGPKAACAGCAGFAAFSV+IEKFL+RHT
Sbjct: 121 -ARAKHDMTNTVVAGCVTGGAISAKGGPKAACAGCAGFAAFSVLIEKFLERHT 170

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BLAST of Carg07043 vs. ExPASy Swiss-Prot
Match: A2RVP7 (Mitochondrial import inner membrane translocase subunit TIM22-1 OS=Arabidopsis thaliana OX=3702 GN=TIM22-1 PE=1 SV=1)

HSP 1 Score: 245.4 bits (625), Expect = 4.9e-64
Identity = 123/172 (71.51%), Postives = 140/172 (81.40%), Query Frame = 0


Query: 2   ASSSGDGSSHDPASSSELEKPPLEPIRMPTVEEIRGQDIWNNCAVRSVVSGVMGGGLGLF 61
           A+    G+S  P +S E     ++PIRMPT+EEIR Q++WNNCAVR+V SGVMGGGLGL 
Sbjct: 6   AAEPTTGASSPPVASDE-NSTQIQPIRMPTIEEIRAQEVWNNCAVRAVTSGVMGGGLGLM 65

Query: 62  MGLFLGALDNPLMQDEMTARQQFVYTAKQMGRRSWGSAKAFAVMGFVFSAAECVVEKFLN 121
           MGLFLGALDNP+  D MTARQQFV+TAKQMG+RSW S K FAVMG VFSAAEC+VEK   
Sbjct: 66  MGLFLGALDNPITHDTMTARQQFVFTAKQMGQRSWNSCKTFAVMGLVFSAAECIVEK--- 125

Query: 122 ARAKHDITNTVVAGCVTGGSISAKGGPKAACAGCAGFAAFSVVIEKFLDRHT 174
           ARAKHD  NT +AGCVTGGS+SA+GGPKAAC GCAGFA FSV+IEKF DRHT
Sbjct: 126 ARAKHDTVNTAIAGCVTGGSMSARGGPKAACIGCAGFATFSVLIEKFFDRHT 173

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BLAST of Carg07043 vs. ExPASy Swiss-Prot
Match: A1XJK0 (Mitochondrial import inner membrane translocase subunit TIM22-4 OS=Arabidopsis thaliana OX=3702 GN=TIM22-4 PE=2 SV=3)

HSP 1 Score: 236.9 bits (603), Expect = 1.7e-61
Identity = 114/145 (78.62%), Postives = 126/145 (86.90%), Query Frame = 0


Query: 29  MPTVEEIRGQDIWNNCAVRSVVSGVMGGGLGLFMGLFLGALDNPLMQDEMTARQQFVYTA 88
           MPT+EEIR Q++WNNCAVR+V SGVMGGGLGL MGLFLGALDNP+  D MTARQQFV+TA
Sbjct: 1   MPTIEEIRAQEVWNNCAVRAVTSGVMGGGLGLMMGLFLGALDNPITHDTMTARQQFVFTA 60

Query: 89  KQMGRRSWGSAKAFAVMGFVFSAAECVVEKFLNARAKHDITNTVVAGCVTGGSISAKGGP 148
           KQMG+RSW S K FAVMG VFSAAEC+VEK   ARAKHD  NT +AGCVTGGS+SA+GGP
Sbjct: 61  KQMGQRSWNSCKTFAVMGLVFSAAECIVEK---ARAKHDTVNTAIAGCVTGGSMSARGGP 120

Query: 149 KAACAGCAGFAAFSVVIEKFLDRHT 174
           KAAC GCAGFA FSV+IEKF DRHT
Sbjct: 121 KAACIGCAGFAIFSVLIEKFFDRHT 142

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BLAST of Carg07043 vs. ExPASy Swiss-Prot
Match: Q2UAP8 (Mitochondrial import inner membrane translocase subunit tim22 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=tim22 PE=3 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 6.4e-24
Identity = 60/134 (44.78%), Postives = 87/134 (64.93%), Query Frame = 0


Query: 43  NCAVRSVVSGVMGGGLGLFMGLFLGAL--DNPLMQD-----EMTARQQFVYTAKQMGRRS 102
           +C V++V+SG MG GLG   GLF+ ++  D+          ++  R+Q     K MG RS
Sbjct: 48  SCPVKTVISGTMGFGLGGVFGLFMASMSYDSTFTPQGKAIMDLPWREQVRRGFKDMGSRS 107

Query: 103 WGSAKAFAVMGFVFSAAECVVEKFLNARAKHDITNTVVAGCVTGGSISAKGGPKAACAGC 162
           W SAK F ++G ++S  EC VE     RAK+D++N+V++GC+TGG + AK GP+AA AGC
Sbjct: 108 WSSAKNFGIVGALYSGTECCVE---GLRAKNDLSNSVISGCITGGILGAKAGPQAAAAGC 167

Query: 163 AGFAAFSVVIEKFL 170
           AGFAAFS  I+ ++
Sbjct: 168 AGFAAFSAAIDAYM 178

[Close]

BLAST of Carg07043 vs. ExPASy Swiss-Prot
Match: A0A1D8PI78 (Mitochondrial import inner membrane translocase subunit TIM22 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=TIM22 PE=1 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 1.1e-23
Identity = 66/164 (40.24%), Postives = 95/164 (57.93%), Query Frame = 0


Query: 21  KPPLEPIRMPTVEEIRGQ------DIWNNCAVRSVVSGVMGGGLGLFMGLFLG--ALDNP 80
           +PP +P++  T EE   +         N+C  ++V++GV G  LG F GLF+   A D P
Sbjct: 11  QPPKKPLQEMTQEEQAEEGARQMIGFMNSCPGKTVMAGVSGFALGGFFGLFMASMAYDTP 70

Query: 81  LMQD------EMTARQQFVYTAKQMGRRSWGSAKAFAVMGFVFSAAECVVEKFLNARAKH 140
           +  D      E+  +QQ       M +RS+ SAK F  +G V+S  EC +E   + RAKH
Sbjct: 71  IGTDAVKHISELPFKQQMKLQFTDMAKRSYSSAKNFGYIGMVYSGVECTIE---SLRAKH 130

Query: 141 DITNTVVAGCVTGGSISAKGGPKAACAGCAGFAAFSVVIEKFLD 171
           DI N V AGC+TG  ++ + GP+AA  GCAGFAAFS+ I+ +L+
Sbjct: 131 DIYNGVSAGCITGAGLAIRAGPQAALVGCAGFAAFSLAIDMYLN 171

[Close]

BLAST of Carg07043 vs. ExPASy Swiss-Prot
Match: Q6BZY4 (Mitochondrial import inner membrane translocase subunit TIM22 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=TIM22 PE=3 SV=2)

HSP 1 Score: 105.5 bits (262), Expect = 6.0e-22
Identity = 60/134 (44.78%), Postives = 82/134 (61.19%), Query Frame = 0


Query: 43  NCAVRSVVSGVMGGGLGLFMGLFLG--ALDNPL-----MQDEMTARQQFVYTAKQMGRRS 102
           +C  +SV++GV G GLG   GLF+   A D P+        ++  +QQ       MG+R+
Sbjct: 41  SCPGKSVMAGVTGFGLGGVFGLFMASMAYDAPVGMGVQTMSDLPFKQQMKIQFTDMGKRA 100

Query: 103 WGSAKAFAVMGFVFSAAECVVEKFLNARAKHDITNTVVAGCVTGGSISAKGGPKAACAGC 162
           W SAK F  +G VFS  EC +E   + RAK+DI N V AGC+TGG ++ K GP+AA  GC
Sbjct: 101 WSSAKNFGFIGGVFSGTECCIE---SLRAKNDIWNGVAAGCLTGGGLAVKAGPQAALVGC 160

Query: 163 AGFAAFSVVIEKFL 170
           AGFAAFS  I+ ++
Sbjct: 161 AGFAAFSAAIDVYM 171

[Close]

BLAST of Carg07043 vs. ExPASy TrEMBL
Match: A0A6J1K8G7 (mitochondrial import inner membrane translocase subunit TIM22-4 OS=Cucurbita maxima OX=3661 GN=LOC111493200 PE=4 SV=1)

HSP 1 Score: 330.1 bits (845), Expect = 5.5e-87
Identity = 170/173 (98.27%), Postives = 170/173 (98.27%), Query Frame = 0


Query: 1   MASSSGDGSSHDPASSSELEKPPLEPIRMPTVEEIRGQDIWNNCAVRSVVSGVMGGGLGL 60
           MASSSGDGSSHDPASSSELEKPPLEPIRMPTVEEIRGQDIWNNCAVRSVVSGVMGGGLGL
Sbjct: 1   MASSSGDGSSHDPASSSELEKPPLEPIRMPTVEEIRGQDIWNNCAVRSVVSGVMGGGLGL 60

Query: 61  FMGLFLGALDNPLMQDEMTARQQFVYTAKQMGRRSWGSAKAFAVMGFVFSAAECVVEKFL 120
           FMGLFLGALDNPLMQDEMTARQQFVYTAKQMGRRSWGSAKAFAVMGFVFSAAECVVEK  
Sbjct: 61  FMGLFLGALDNPLMQDEMTARQQFVYTAKQMGRRSWGSAKAFAVMGFVFSAAECVVEK-- 120

Query: 121 NARAKHDITNTVVAGCVTGGSISAKGGPKAACAGCAGFAAFSVVIEKFLDRHT 174
            ARAKHDITNTVVAGCVTGGSISAKGGPKAACAGCAGFAAFSVVIEKFLDRHT
Sbjct: 121 -ARAKHDITNTVVAGCVTGGSISAKGGPKAACAGCAGFAAFSVVIEKFLDRHT 170

[Close]

BLAST of Carg07043 vs. ExPASy TrEMBL
Match: A0A6J1G8S5 (mitochondrial import inner membrane translocase subunit TIM22-4 OS=Cucurbita moschata OX=3662 GN=LOC111452007 PE=4 SV=1)

HSP 1 Score: 330.1 bits (845), Expect = 5.5e-87
Identity = 170/173 (98.27%), Postives = 170/173 (98.27%), Query Frame = 0


Query: 1   MASSSGDGSSHDPASSSELEKPPLEPIRMPTVEEIRGQDIWNNCAVRSVVSGVMGGGLGL 60
           MASSSGDGSSHDPASSSELEKPPLEPIRMPTVEEIRGQDIWNNCAVRSVVSGVMGGGLGL
Sbjct: 1   MASSSGDGSSHDPASSSELEKPPLEPIRMPTVEEIRGQDIWNNCAVRSVVSGVMGGGLGL 60

Query: 61  FMGLFLGALDNPLMQDEMTARQQFVYTAKQMGRRSWGSAKAFAVMGFVFSAAECVVEKFL 120
           FMGLFLGALDNPLMQDEMTARQQFVYTAKQMGRRSWGSAKAFAVMGFVFSAAECVVEK  
Sbjct: 61  FMGLFLGALDNPLMQDEMTARQQFVYTAKQMGRRSWGSAKAFAVMGFVFSAAECVVEK-- 120

Query: 121 NARAKHDITNTVVAGCVTGGSISAKGGPKAACAGCAGFAAFSVVIEKFLDRHT 174
            ARAKHDITNTVVAGCVTGGSISAKGGPKAACAGCAGFAAFSVVIEKFLDRHT
Sbjct: 121 -ARAKHDITNTVVAGCVTGGSISAKGGPKAACAGCAGFAAFSVVIEKFLDRHT 170

[Close]

BLAST of Carg07043 vs. ExPASy TrEMBL
Match: A0A6J1DKY7 (mitochondrial import inner membrane translocase subunit TIM22-4-like OS=Momordica charantia OX=3673 GN=LOC111022051 PE=4 SV=1)

HSP 1 Score: 309.7 bits (792), Expect = 7.8e-81
Identity = 154/173 (89.02%), Postives = 167/173 (96.53%), Query Frame = 0


Query: 1   MASSSGDGSSHDPASSSELEKPPLEPIRMPTVEEIRGQDIWNNCAVRSVVSGVMGGGLGL 60
           MA+++G+GSSHDP SS+E EKPP+EPIRMPTVEEIRGQDIWNNCAVRSVVSGVMGGGLGL
Sbjct: 1   MATNAGNGSSHDPTSSNEAEKPPIEPIRMPTVEEIRGQDIWNNCAVRSVVSGVMGGGLGL 60

Query: 61  FMGLFLGALDNPLMQDEMTARQQFVYTAKQMGRRSWGSAKAFAVMGFVFSAAECVVEKFL 120
           FMGLFLGALDNPLMQ+EMTA+QQF++TAKQMGRRSWGSAKAFAVMGF+FSAAECVVEK  
Sbjct: 61  FMGLFLGALDNPLMQEEMTAKQQFIFTAKQMGRRSWGSAKAFAVMGFIFSAAECVVEK-- 120

Query: 121 NARAKHDITNTVVAGCVTGGSISAKGGPKAACAGCAGFAAFSVVIEKFLDRHT 174
            ARAKHD TNTVVAGCVTGGSISAKGGPKAACAGCAGFAAFSV+IEKFLDRH+
Sbjct: 121 -ARAKHDTTNTVVAGCVTGGSISAKGGPKAACAGCAGFAAFSVLIEKFLDRHS 170

[Close]

BLAST of Carg07043 vs. ExPASy TrEMBL
Match: A0A1S3C5B1 (mitochondrial import inner membrane translocase subunit TIM22-4-like OS=Cucumis melo OX=3656 GN=LOC103497039 PE=4 SV=1)

HSP 1 Score: 292.7 bits (748), Expect = 9.8e-76
Identity = 145/173 (83.82%), Postives = 163/173 (94.22%), Query Frame = 0


Query: 1   MASSSGDGSSHDPASSSELEKPPLEPIRMPTVEEIRGQDIWNNCAVRSVVSGVMGGGLGL 60
           MAS+SGDG S DP +S++ E+PP+ P+RMPTVEEIRGQDIW+NCAVRSVVSGVMGGG+GL
Sbjct: 1   MASTSGDGFSPDPPTSNDAEQPPIAPMRMPTVEEIRGQDIWDNCAVRSVVSGVMGGGIGL 60

Query: 61  FMGLFLGALDNPLMQDEMTARQQFVYTAKQMGRRSWGSAKAFAVMGFVFSAAECVVEKFL 120
           F+GLFLG++DN LMQDEMTARQQFVYTAKQMGRR WGSAK+FAVMGF+FSAAECVVEK  
Sbjct: 61  FLGLFLGSMDNSLMQDEMTARQQFVYTAKQMGRRGWGSAKSFAVMGFIFSAAECVVEK-- 120

Query: 121 NARAKHDITNTVVAGCVTGGSISAKGGPKAACAGCAGFAAFSVVIEKFLDRHT 174
            ARAKHD+TNTVVAGCVTGG+ISAKGGPKAACAGCAGFAAFSV+IEKFL+RHT
Sbjct: 121 -ARAKHDMTNTVVAGCVTGGAISAKGGPKAACAGCAGFAAFSVLIEKFLERHT 170

[Close]

BLAST of Carg07043 vs. ExPASy TrEMBL
Match: A0A5B7AJU4 (Uncharacterized protein OS=Davidia involucrata OX=16924 GN=Din_026035 PE=4 SV=1)

HSP 1 Score: 285.0 bits (728), Expect = 2.0e-73
Identity = 140/172 (81.40%), Postives = 160/172 (93.02%), Query Frame = 0


Query: 1   MASSSGDGSSHDPASSSELEKPPLEPIRMPTVEEIRGQDIWNNCAVRSVVSGVMGGGLGL 60
           M+++SG  S++  ++S+E +KP +EP+R+PTVEEIRGQDIWNNCAVRSVVSGVMGGGLGL
Sbjct: 18  MSANSGTESTNGSSNSNEAQKPQIEPLRLPTVEEIRGQDIWNNCAVRSVVSGVMGGGLGL 77

Query: 61  FMGLFLGALDNPLMQDEMTARQQFVYTAKQMGRRSWGSAKAFAVMGFVFSAAECVVEKFL 120
           FMGLFLGALDNPLMQ+EMT RQQF+YTAKQMGRRSWGS K FAVMGF+FSAAECVVEK  
Sbjct: 78  FMGLFLGALDNPLMQEEMTGRQQFIYTAKQMGRRSWGSCKTFAVMGFIFSAAECVVEK-- 137

Query: 121 NARAKHDITNTVVAGCVTGGSISAKGGPKAACAGCAGFAAFSVVIEKFLDRH 173
            ARAKHD+TNTVVAGCVTGG++SAKGGPKAACAGCAGFAAFSV+IEKFL+RH
Sbjct: 138 -ARAKHDVTNTVVAGCVTGGALSAKGGPKAACAGCAGFAAFSVLIEKFLERH 186

[Close]

BLAST of Carg07043 vs. TAIR 10
Match: AT3G10110.1 (Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family protein )

HSP 1 Score: 245.4 bits (625), Expect = 3.5e-65
Identity = 123/172 (71.51%), Postives = 140/172 (81.40%), Query Frame = 0


Query: 2   ASSSGDGSSHDPASSSELEKPPLEPIRMPTVEEIRGQDIWNNCAVRSVVSGVMGGGLGLF 61
           A+    G+S  P +S E     ++PIRMPT+EEIR Q++WNNCAVR+V SGVMGGGLGL 
Sbjct: 6   AAEPTTGASSPPVASDE-NSTQIQPIRMPTIEEIRAQEVWNNCAVRAVTSGVMGGGLGLM 65

Query: 62  MGLFLGALDNPLMQDEMTARQQFVYTAKQMGRRSWGSAKAFAVMGFVFSAAECVVEKFLN 121
           MGLFLGALDNP+  D MTARQQFV+TAKQMG+RSW S K FAVMG VFSAAEC+VEK   
Sbjct: 66  MGLFLGALDNPITHDTMTARQQFVFTAKQMGQRSWNSCKTFAVMGLVFSAAECIVEK--- 125

Query: 122 ARAKHDITNTVVAGCVTGGSISAKGGPKAACAGCAGFAAFSVVIEKFLDRHT 174
           ARAKHD  NT +AGCVTGGS+SA+GGPKAAC GCAGFA FSV+IEKF DRHT
Sbjct: 126 ARAKHDTVNTAIAGCVTGGSMSARGGPKAACIGCAGFATFSVLIEKFFDRHT 173

[Close]

BLAST of Carg07043 vs. TAIR 10
Match: AT1G18320.1 (Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family protein )

HSP 1 Score: 236.9 bits (603), Expect = 1.2e-62
Identity = 114/145 (78.62%), Postives = 126/145 (86.90%), Query Frame = 0


Query: 29  MPTVEEIRGQDIWNNCAVRSVVSGVMGGGLGLFMGLFLGALDNPLMQDEMTARQQFVYTA 88
           MPT+EEIR Q++WNNCAVR+V SGVMGGGLGL MGLFLGALDNP+  D MTARQQFV+TA
Sbjct: 1   MPTIEEIRAQEVWNNCAVRAVTSGVMGGGLGLMMGLFLGALDNPITHDTMTARQQFVFTA 60

Query: 89  KQMGRRSWGSAKAFAVMGFVFSAAECVVEKFLNARAKHDITNTVVAGCVTGGSISAKGGP 148
           KQMG+RSW S K FAVMG VFSAAEC+VEK   ARAKHD  NT +AGCVTGGS+SA+GGP
Sbjct: 61  KQMGQRSWNSCKTFAVMGLVFSAAECIVEK---ARAKHDTVNTAIAGCVTGGSMSARGGP 120

Query: 149 KAACAGCAGFAAFSVVIEKFLDRHT 174
           KAAC GCAGFA FSV+IEKF DRHT
Sbjct: 121 KAACIGCAGFAIFSVLIEKFFDRHT 142

The following BLAST results are available for this feature:

BLAST of Carg07043 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7037014.1	2.9e-90	100.00	Mitochondrial import inner membrane translocase subunit TIM22-1 [Cucurbita argyr...	[more]
XP_022948292.1	1.1e-86	98.27	mitochondrial import inner membrane translocase subunit TIM22-4 [Cucurbita mosch...	[more]
XP_022154905.1	1.6e-80	89.02	mitochondrial import inner membrane translocase subunit TIM22-4-like [Momordica ...	[more]
XP_038893922.1	5.2e-79	89.02	mitochondrial import inner membrane translocase subunit TIM22-4-like [Benincasa ...	[more]
XP_008457318.1	2.0e-75	83.82	PREDICTED: mitochondrial import inner membrane translocase subunit TIM22-4-like ...	[more]

BLAST of Carg07043 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A2RVP7	4.9e-64	71.51	Mitochondrial import inner membrane translocase subunit TIM22-1 OS=Arabidopsis t...	[more]
A1XJK0	1.7e-61	78.62	Mitochondrial import inner membrane translocase subunit TIM22-4 OS=Arabidopsis t...	[more]
Q2UAP8	6.4e-24	44.78	Mitochondrial import inner membrane translocase subunit tim22 OS=Aspergillus ory...	[more]
A0A1D8PI78	1.1e-23	40.24	Mitochondrial import inner membrane translocase subunit TIM22 OS=Candida albican...	[more]
Q6BZY4	6.0e-22	44.78	Mitochondrial import inner membrane translocase subunit TIM22 OS=Yarrowia lipoly...	[more]

BLAST of Carg07043 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1K8G7	5.5e-87	98.27	mitochondrial import inner membrane translocase subunit TIM22-4 OS=Cucurbita max...	[more]
A0A6J1G8S5	5.5e-87	98.27	mitochondrial import inner membrane translocase subunit TIM22-4 OS=Cucurbita mos...	[more]
A0A6J1DKY7	7.8e-81	89.02	mitochondrial import inner membrane translocase subunit TIM22-4-like OS=Momordic...	[more]
A0A1S3C5B1	9.8e-76	83.82	mitochondrial import inner membrane translocase subunit TIM22-4-like OS=Cucumis ...	[more]
A0A5B7AJU4	2.0e-73	81.40	Uncharacterized protein OS=Davidia involucrata OX=16924 GN=Din_026035 PE=4 SV=1	[more]

BLAST of Carg07043 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G10110.1	3.5e-65	71.51	Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family...	[more]
AT1G18320.1	1.2e-62	78.62	Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PFAM	PF02466	Tim17	coord: 44..162 e-value: 2.5E-28 score: 98.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..16
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..26
None	No IPR available	PANTHER	PTHR14110:SF0	MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM22	coord: 17..172
IPR039175	Mitochondrial import inner membrane translocase subunit TIM22	PANTHER	PTHR14110	MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM22	coord: 17..172

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg07043-RA	Carg07043-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009793	embryo development ending in seed dormancy
biological_process	GO:0045039	protein insertion into mitochondrial inner membrane
biological_process	GO:0071806	protein transmembrane transport
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0042721	TIM22 mitochondrial import inner membrane insertion complex
molecular_function	GO:0030943	mitochondrion targeting sequence binding
molecular_function	GO:0008320	protein transmembrane transporter activity