Carg07027 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg07027
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionRNA-directed DNA methylation 4
LocationCarg_Chr01: 3841384 .. 3844418 (-)
RNA-Seq ExpressionCarg07027
SyntenyCarg07027
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAGAAGTTCGTAGTTGTTCTCCGGCTCGCATGTTCTAGACCCTGAGCGTCGATCGTGTCTCCGATCGCTTCGCATATGGCCAGCATCGGCGAGAGTTCTTCTTCTGCTCCCAAATTCGCGGACGAAAAGCCAGTCTTAGTTAGGGTTAAGCGCAAAGCTTCCCAGTCTCGACTCGATGCGTTATGTGAGTTCTCTTCGGACGCTCGTTTTCATTCACTTCATCAAGGCCTTCATCTTGCTGATTTTAGTTGATGAATCGCCTTTACATTATTGTAATTTTTGGATATTAGCCGTCTCTTTTGATTTCTAACTATTCACGTATCAAATGGAGTTTGAATTGTTCGTTTCCGAGTTGACGTTCGGATAATTTGTATGTTGCAGGGCTGGAAATCAATGAGAGGCCACTGAAACGACCTCTTTTGGATTTTGATAATTTGTCTATTTCTGAAACGTTCCAGAAAGGTTTGATAATTTCTGAACCTATAATTGCGTTCTTTTCTTTTCACCAATCACTCTATAATCTACATAGGTGAAAAGAGGAAACAGCAGTTAGTAGCTATCTATGATTCTTCCACAAGAATATATAGTAACTGCTCCTGATTATACCCTGGTGTTTGCAGGGCTAGTGATTTTAAAAAATACAAACATGGATTCTTTGTCCCTTCATAAGAAAATTAATGGATGGATTTCATTGTTGTTATTGTTTCTTCTTTTTCTGTGAGGGAGGGGTGAATGGATTTATTTACTTTACTGTTCTTGCCAATTGATTAAATGTAAACTTTGATAGTTCGACCAAAATTGTACTGGATTTCTCAGTTGCATGGGTCAATGAAGTATATTTAAATATGGCTGTGTATAACTCCGTCCCTGATGGAATAGCTTTCTCTTGCATCAGAGGAACTTAAAACTAAGAAGATTTTTGTACAGCATGTGGAGACACTGAGAAGCTCTGAGGCCTCAGTTGACATTATTCAGTCGTTCGTGGTTAGTATTGTGGTCAGTTATTAATCTCCTTCTTGGACATTTTGACTAGCATCTTCATCCCTTGCTGTTTCCCCTTTAGGCACCAGATGCTGCTGGCAACATTGAAAATAACCTAAAGAATGAGGAGCGCAGAAGAAATTTTAAGAGAGAGAGTGTATGCTGTCCTGTCTGTTGTTTGTTTGGTTCTTAGTTGTGCGGTACTTGTTGAATAATTTTCTCACTTTATCTAGTGTTCTGCAGCCAAGACAAGAACAGCTGTTGGTTAAAACTAGACAAGAACAAGAGGTCTGGTCTATGATGTCTTTTCTTTAATGCTTTTTCTTAAAATAGATATAATCAATGTTTTACCTGAAGAATTTGTCAGACTAGATTATAGATGGCATTCTCTAGTTGGACCAAGTTTTGTTCATATGTTGAACTGTTATATTATGTTGGTAACATGGTTTCTTTGTGCTACTGCTTTCTCCTTTTACTAGGTTTTGGCAAAAAAAGCTAGATTTGAGCAAATATGGAGAAGTAGGAAAGGGATTAAAGATCCCAAAGACGACCAATTACGTGACGCATATCATATTTATGATATTGTTCGTCTTGATACAAATGAAACATCAAGTGAAGCACCAAAGGCGGAGTTAGTTCTTTAGTTCTGTCATTATGATATTGTTTGGTCATGATATTCTATAAGATCCTCATTTATGTGGCCATATTTTCTTTCGGTATTAATGCAGGTGTATGACTCTGGAGGATCAAAGCATGTTATCAAGTTATCTGCCTTTACTAAGAGAGTTTATTCCAAGTGCTGCTGAAGAGATTGAGTCAGATATAAATGCAAACACGATGAAACAAGATTGTAAGTATCATTTAATCCAGCAGTCACTTGAGGTATTTTGCTTGCAACTGTAGCTATCCTCCATTTTCCATTTGTTTTCCAGTTCTTGCCATGCCTGAGTTTCCTTCCTAGCTCACCCTTTTGTTATATCATTTGTATGTTCATAGGGCTGTCGTTACTGTAATTTCTTATTGCAATGTCTGTGTTTTCACTTGTTCTACAGTGCCCGTAGATGATTATGTATATGACTACTATACTGTGAAGAATAATGTTCCGATTGCCGAGGACGATGCCTCTAATCCATTTCCTTTGTGCGTTATTCTCTCGCTCTCTCTCTGTTTTCCCTCTCTGCTCTGGGTTATACTTGATTTTATTTTTTTGGAAATCATTTCACATATTTTTTCAGGATACAAGTCGACGACTTGGATCTATATGATGGGCCTGATGACTCGGACTATGAAAGTGATGATTCAAATGGTAAGTAGGTTTTTCCCTCTAGTTTAGAAACCTAAATTATAACTAATGTCTGTTGTTTCTAATATCTTTTTTGGTTTCAGCTGAAAACAATCCACACTTCGATTACCCAGATGAGGCGTCCGAAGAAGAAGAGTTGGAAAGTGAATCTACAAATGAAGAATCAGAAGAAGATGATGAGGACAAGCAGTCCTCAGAAGCTAATGATCTAGAAGAGGACGACCTGTCAGAGAATGGAGTTCAATTAGACGACGATGAAGTATATGGTGATTTTGACGAAGACGATGGCGATAGTTTTGATTACGATAGTAATGGTGGTCACGGAGAGGGTGAAGATTGGAGATGGTCCTACCGTTGAATTTTTGGTATTATAGGACAGCTTTTTTCGTCTTTGGGAGAGGGATGTTTGTTCTACCGCTTGTCCTATATCAGGTATTACCATTTTCCTTAAGTGAGTTGCTTAATAGGGAGAGGAATGTGCTTGTAATGACTAGGGATGTTCCTTTGGAGATTTAGAGGTCAAAATACACTTTGCTTTGCTACTTCAATAGAAATTAAAAGCCCTGTAGGCCTCCTTTGGTAATATATTCACCGTTCTCTGTTTCTTGTTGAGAAACAGATGTGTCGGGTTCTTGTTTCTAAAACGTTTCCAAAATAGACCAAAATCTGAGAAACTTTCTTTGTTATGTTCCTTCCCAACTTTCCTATCTTCAGCAGATTTCTTCTCTCTCACGCTCACCAAGAACGAAC

mRNA sequence

CAAGAAGTTCGTAGTTGTTCTCCGGCTCGCATGTTCTAGACCCTGAGCGTCGATCGTGTCTCCGATCGCTTCGCATATGGCCAGCATCGGCGAGAGTTCTTCTTCTGCTCCCAAATTCGCGGACGAAAAGCCAGTCTTAGTTAGGGTTAAGCGCAAAGCTTCCCAGTCTCGACTCGATGCGTTATGGCTGGAAATCAATGAGAGGCCACTGAAACGACCTCTTTTGGATTTTGATAATTTGTCTATTTCTGAAACGTTCCAGAAAGAGGAACTTAAAACTAAGAAGATTTTTGTACAGCATGTGGAGACACTGAGAAGCTCTGAGGCCTCAGTTGACATTATTCAGTCGTTCGTGGCACCAGATGCTGCTGGCAACATTGAAAATAACCTAAAGAATGAGGAGCGCAGAAGAAATTTTAAGAGAGAGAGTCCAAGACAAGAACAGCTGTTGGTTAAAACTAGACAAGAACAAGAGGTTTTGGCAAAAAAAGCTAGATTTGAGCAAATATGGAGAAGTAGGAAAGGGATTAAAGATCCCAAAGACGACCAATTACGTGACGCATATCATATTTATGATATTGTTCGTCTTGATACAAATGAAACATCAAGTGAAGCACCAAAGGCGGAGTGTATGACTCTGGAGGATCAAAGCATGTTATCAAGTTATCTGCCTTTACTAAGAGAGTTTATTCCAAGTGCTGCTGAAGAGATTGAGTCAGATATAAATGCAAACACGATGAAACAAGATTGTAAGTATCATTTAATCCAGCAGTCACTTGAGGTATTTTGCTTGCAACTGGCTGTCGTTACTGTAATTTCTTATTGCAATGTCTGTGTTTTCACTTGTTCTACAGTGCCCGTAGATGATTATGTATATGACTACTATACTGTGAAGAATAATGTTCCGATTGCCGAGGACGATGCCTCTAATCCATTTCCTTTGATACAAGTCGACGACTTGGATCTATATGATGGGCCTGATGACTCGGACTATGAAAGTGATGATTCAAATGCTGAAAACAATCCACACTTCGATTACCCAGATGAGGCGTCCGAAGAAGAAGAGTTGGAAAGTGAATCTACAAATGAAGAATCAGAAGAAGATGATGAGGACAAGCAGTCCTCAGAAGCTAATGATCTAGAAGAGGACGACCTGTCAGAGAATGGAGTTCAATTAGACGACGATGAAGTATATGGTGATTTTGACGAAGACGATGGCGATAGTTTTGATTACGATAGTAATGGTGGTCACGGAGAGGGTGAAGATTGGAGATGGTCCTACCGTTGAATTTTTGGTATTATAGGACAGCTTTTTTCGTCTTTGGGAGAGGGATGTTTGTTCTACCGCTTGTCCTATATCAGGTATTACCATTTTCCTTAAGTGAGTTGCTTAATAGGGAGAGGAATGTGCTTGTAATGACTAGGGATGTTCCTTTGGAGATTTAGAGGTCAAAATACACTTTGCTTTGCTACTTCAATAGAAATTAAAAGCCCTGTAGGCCTCCTTTGGTAATATATTCACCGTTCTCTGTTTCTTGTTGAGAAACAGATGTGTCGGGTTCTTGTTTCTAAAACGTTTCCAAAATAGACCAAAATCTGAGAAACTTTCTTTGTTATGTTCCTTCCCAACTTTCCTATCTTCAGCAGATTTCTTCTCTCTCACGCTCACCAAGAACGAAC

Coding sequence (CDS)

ATGGCCAGCATCGGCGAGAGTTCTTCTTCTGCTCCCAAATTCGCGGACGAAAAGCCAGTCTTAGTTAGGGTTAAGCGCAAAGCTTCCCAGTCTCGACTCGATGCGTTATGGCTGGAAATCAATGAGAGGCCACTGAAACGACCTCTTTTGGATTTTGATAATTTGTCTATTTCTGAAACGTTCCAGAAAGAGGAACTTAAAACTAAGAAGATTTTTGTACAGCATGTGGAGACACTGAGAAGCTCTGAGGCCTCAGTTGACATTATTCAGTCGTTCGTGGCACCAGATGCTGCTGGCAACATTGAAAATAACCTAAAGAATGAGGAGCGCAGAAGAAATTTTAAGAGAGAGAGTCCAAGACAAGAACAGCTGTTGGTTAAAACTAGACAAGAACAAGAGGTTTTGGCAAAAAAAGCTAGATTTGAGCAAATATGGAGAAGTAGGAAAGGGATTAAAGATCCCAAAGACGACCAATTACGTGACGCATATCATATTTATGATATTGTTCGTCTTGATACAAATGAAACATCAAGTGAAGCACCAAAGGCGGAGTGTATGACTCTGGAGGATCAAAGCATGTTATCAAGTTATCTGCCTTTACTAAGAGAGTTTATTCCAAGTGCTGCTGAAGAGATTGAGTCAGATATAAATGCAAACACGATGAAACAAGATTGTAAGTATCATTTAATCCAGCAGTCACTTGAGGTATTTTGCTTGCAACTGGCTGTCGTTACTGTAATTTCTTATTGCAATGTCTGTGTTTTCACTTGTTCTACAGTGCCCGTAGATGATTATGTATATGACTACTATACTGTGAAGAATAATGTTCCGATTGCCGAGGACGATGCCTCTAATCCATTTCCTTTGATACAAGTCGACGACTTGGATCTATATGATGGGCCTGATGACTCGGACTATGAAAGTGATGATTCAAATGCTGAAAACAATCCACACTTCGATTACCCAGATGAGGCGTCCGAAGAAGAAGAGTTGGAAAGTGAATCTACAAATGAAGAATCAGAAGAAGATGATGAGGACAAGCAGTCCTCAGAAGCTAATGATCTAGAAGAGGACGACCTGTCAGAGAATGGAGTTCAATTAGACGACGATGAAGTATATGGTGATTTTGACGAAGACGATGGCGATAGTTTTGATTACGATAGTAATGGTGGTCACGGAGAGGGTGAAGATTGGAGATGGTCCTACCGTTGA

Protein sequence

MASIGESSSSAPKFADEKPVLVRVKRKASQSRLDALWLEINERPLKRPLLDFDNLSISETFQKEELKTKKIFVQHVETLRSSEASVDIIQSFVAPDAAGNIENNLKNEERRRNFKRESPRQEQLLVKTRQEQEVLAKKARFEQIWRSRKGIKDPKDDQLRDAYHIYDIVRLDTNETSSEAPKAECMTLEDQSMLSSYLPLLREFIPSAAEEIESDINANTMKQDCKYHLIQQSLEVFCLQLAVVTVISYCNVCVFTCSTVPVDDYVYDYYTVKNNVPIAEDDASNPFPLIQVDDLDLYDGPDDSDYESDDSNAENNPHFDYPDEASEEEELESESTNEESEEDDEDKQSSEANDLEEDDLSENGVQLDDDEVYGDFDEDDGDSFDYDSNGGHGEGEDWRWSYR
Homology
BLAST of Carg07027 vs. NCBI nr
Match: KAG7037030.1 (RNA-directed DNA methylation 4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 755.4 bits (1949), Expect = 2.6e-214
Identity = 403/403 (100.00%), Postives = 403/403 (100.00%), Query Frame = 0

Query: 1   MASIGESSSSAPKFADEKPVLVRVKRKASQSRLDALWLEINERPLKRPLLDFDNLSISET 60
           MASIGESSSSAPKFADEKPVLVRVKRKASQSRLDALWLEINERPLKRPLLDFDNLSISET
Sbjct: 1   MASIGESSSSAPKFADEKPVLVRVKRKASQSRLDALWLEINERPLKRPLLDFDNLSISET 60

Query: 61  FQKEELKTKKIFVQHVETLRSSEASVDIIQSFVAPDAAGNIENNLKNEERRRNFKRESPR 120
           FQKEELKTKKIFVQHVETLRSSEASVDIIQSFVAPDAAGNIENNLKNEERRRNFKRESPR
Sbjct: 61  FQKEELKTKKIFVQHVETLRSSEASVDIIQSFVAPDAAGNIENNLKNEERRRNFKRESPR 120

Query: 121 QEQLLVKTRQEQEVLAKKARFEQIWRSRKGIKDPKDDQLRDAYHIYDIVRLDTNETSSEA 180
           QEQLLVKTRQEQEVLAKKARFEQIWRSRKGIKDPKDDQLRDAYHIYDIVRLDTNETSSEA
Sbjct: 121 QEQLLVKTRQEQEVLAKKARFEQIWRSRKGIKDPKDDQLRDAYHIYDIVRLDTNETSSEA 180

Query: 181 PKAECMTLEDQSMLSSYLPLLREFIPSAAEEIESDINANTMKQDCKYHLIQQSLEVFCLQ 240
           PKAECMTLEDQSMLSSYLPLLREFIPSAAEEIESDINANTMKQDCKYHLIQQSLEVFCLQ
Sbjct: 181 PKAECMTLEDQSMLSSYLPLLREFIPSAAEEIESDINANTMKQDCKYHLIQQSLEVFCLQ 240

Query: 241 LAVVTVISYCNVCVFTCSTVPVDDYVYDYYTVKNNVPIAEDDASNPFPLIQVDDLDLYDG 300
           LAVVTVISYCNVCVFTCSTVPVDDYVYDYYTVKNNVPIAEDDASNPFPLIQVDDLDLYDG
Sbjct: 241 LAVVTVISYCNVCVFTCSTVPVDDYVYDYYTVKNNVPIAEDDASNPFPLIQVDDLDLYDG 300

Query: 301 PDDSDYESDDSNAENNPHFDYPDEASEEEELESESTNEESEEDDEDKQSSEANDLEEDDL 360
           PDDSDYESDDSNAENNPHFDYPDEASEEEELESESTNEESEEDDEDKQSSEANDLEEDDL
Sbjct: 301 PDDSDYESDDSNAENNPHFDYPDEASEEEELESESTNEESEEDDEDKQSSEANDLEEDDL 360

Query: 361 SENGVQLDDDEVYGDFDEDDGDSFDYDSNGGHGEGEDWRWSYR 404
           SENGVQLDDDEVYGDFDEDDGDSFDYDSNGGHGEGEDWRWSYR
Sbjct: 361 SENGVQLDDDEVYGDFDEDDGDSFDYDSNGGHGEGEDWRWSYR 403

BLAST of Carg07027 vs. NCBI nr
Match: KAG6607356.1 (RNA-directed DNA methylation 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 659.8 bits (1701), Expect = 1.5e-185
Identity = 363/403 (90.07%), Postives = 366/403 (90.82%), Query Frame = 0

Query: 1   MASIGESSSSAPKFADEKPVLVRVKRKASQSRLDALWLEINERPLKRPLLDFDNLSISET 60
           MASIGESSSSAPKFADEKPVLVRVKRKASQSRLDALWLEINERPLKRPLLDFDNLSISET
Sbjct: 1   MASIGESSSSAPKFADEKPVLVRVKRKASQSRLDALWLEINERPLKRPLLDFDNLSISET 60

Query: 61  FQKEELKTKKIFVQHVETLRSSEASVDIIQSFVAPDAAGNIENNLKNEERRRNFKRESPR 120
           FQKEELKTKKIFVQHVETLRSSEASVDIIQSFVAPDAAGNIENNLKNEERRRNFKRESPR
Sbjct: 61  FQKEELKTKKIFVQHVETLRSSEASVDIIQSFVAPDAAGNIENNLKNEERRRNFKRESPR 120

Query: 121 QEQLLVKTRQEQEVLAKKARFEQIWRSRKGIKDPKDDQLRDAYHIYDIVRLDTNETSSEA 180
           QEQLLVKTRQEQEVLAKKARFEQIWRSRKGIKDPKDDQL DAYHIYDIVRLDTNETSSEA
Sbjct: 121 QEQLLVKTRQEQEVLAKKARFEQIWRSRKGIKDPKDDQLHDAYHIYDIVRLDTNETSSEA 180

Query: 181 PKAECMTLEDQSMLSSYLPLLREFIPSAAEEIESDINANTMKQDCKYHLIQQSLEVFCLQ 240
           PKAECMTLEDQSMLSSYLPLLREFIPSAAEEIESDINANTMKQD                
Sbjct: 181 PKAECMTLEDQSMLSSYLPLLREFIPSAAEEIESDINANTMKQD---------------- 240

Query: 241 LAVVTVISYCNVCVFTCSTVPVDDYVYDYYTVKNNVPIAEDDASNPFPLIQVDDLDLYDG 300
                              +PVDDYVYDYYTVKNNVPIAEDDASNPFPLIQVDDLDLYDG
Sbjct: 241 -------------------LPVDDYVYDYYTVKNNVPIAEDDASNPFPLIQVDDLDLYDG 300

Query: 301 PDDSDYESDDSNAENNPHFDYPDEASEEEELESESTNEESEEDDEDKQSSEANDLEEDDL 360
           PDDSDYESDDSNAENNPHFDYPDEASEEEELESESTNEESEEDD+DKQSSEANDLEED+L
Sbjct: 301 PDDSDYESDDSNAENNPHFDYPDEASEEEELESESTNEESEEDDDDKQSSEANDLEEDNL 360

Query: 361 SENGVQLDDDEVYGDFDEDDGDSFDYDSNGGHGEGEDWRWSYR 404
           SENGVQLDDDEVYGDFDEDD DSFDYDSNGGHGEGEDWRWSYR
Sbjct: 361 SENGVQLDDDEVYGDFDEDDDDSFDYDSNGGHGEGEDWRWSYR 368

BLAST of Carg07027 vs. NCBI nr
Match: XP_023522069.1 (RNA-directed DNA methylation 4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 656.8 bits (1693), Expect = 1.2e-184
Identity = 360/403 (89.33%), Postives = 366/403 (90.82%), Query Frame = 0

Query: 1   MASIGESSSSAPKFADEKPVLVRVKRKASQSRLDALWLEINERPLKRPLLDFDNLSISET 60
           MASIGESSSSAPKFADEKPVLVRVKRKASQSRLDALWLEINERPLKRPLLDFDNLSISET
Sbjct: 1   MASIGESSSSAPKFADEKPVLVRVKRKASQSRLDALWLEINERPLKRPLLDFDNLSISET 60

Query: 61  FQKEELKTKKIFVQHVETLRSSEASVDIIQSFVAPDAAGNIENNLKNEERRRNFKRESPR 120
           FQKEELKTKKIFVQHVETLRSSEASVDIIQSFVAPDAAGNIENNLKNEERRRNFKRESPR
Sbjct: 61  FQKEELKTKKIFVQHVETLRSSEASVDIIQSFVAPDAAGNIENNLKNEERRRNFKRESPR 120

Query: 121 QEQLLVKTRQEQEVLAKKARFEQIWRSRKGIKDPKDDQLRDAYHIYDIVRLDTNETSSEA 180
           QEQLLVKTRQEQEVLAKKARFEQIWRSRKGIKDPKDDQL DAYHIYDIVRLDTNETSSEA
Sbjct: 121 QEQLLVKTRQEQEVLAKKARFEQIWRSRKGIKDPKDDQLHDAYHIYDIVRLDTNETSSEA 180

Query: 181 PKAECMTLEDQSMLSSYLPLLREFIPSAAEEIESDINANTMKQDCKYHLIQQSLEVFCLQ 240
           PKAECMTLEDQSMLSSYLPLLREFIPSAAEEIESDINANT+KQD                
Sbjct: 181 PKAECMTLEDQSMLSSYLPLLREFIPSAAEEIESDINANTLKQD---------------- 240

Query: 241 LAVVTVISYCNVCVFTCSTVPVDDYVYDYYTVKNNVPIAEDDASNPFPLIQVDDLDLYDG 300
                              +PVDDYVYDYYTVKNNVPIA+DDASNPFPLIQVDDLDLYDG
Sbjct: 241 -------------------LPVDDYVYDYYTVKNNVPIADDDASNPFPLIQVDDLDLYDG 300

Query: 301 PDDSDYESDDSNAENNPHFDYPDEASEEEELESESTNEESEEDDEDKQSSEANDLEEDDL 360
           PDDS+YESDDSNAENNPHFDYPDEASEEEELESESTNEESEEDDEDKQSSEANDLEEDDL
Sbjct: 301 PDDSEYESDDSNAENNPHFDYPDEASEEEELESESTNEESEEDDEDKQSSEANDLEEDDL 360

Query: 361 SENGVQLDDDEVYGDFDEDDGDSFDYDSNGGHGEGEDWRWSYR 404
           SENGVQLD+DE+YGDFDEDDGDSFDYDSNGGH EGEDWRWSYR
Sbjct: 361 SENGVQLDEDEIYGDFDEDDGDSFDYDSNGGHEEGEDWRWSYR 368

BLAST of Carg07027 vs. NCBI nr
Match: XP_022949593.1 (RNA-directed DNA methylation 4 [Cucurbita moschata])

HSP 1 Score: 653.7 bits (1685), Expect = 1.1e-183
Identity = 360/403 (89.33%), Postives = 364/403 (90.32%), Query Frame = 0

Query: 1   MASIGESSSSAPKFADEKPVLVRVKRKASQSRLDALWLEINERPLKRPLLDFDNLSISET 60
           MASIGESSSSAPKFADEKPVLVRVKRKASQSRLDALWLEINERPLKRPLLDFDNLSISET
Sbjct: 1   MASIGESSSSAPKFADEKPVLVRVKRKASQSRLDALWLEINERPLKRPLLDFDNLSISET 60

Query: 61  FQKEELKTKKIFVQHVETLRSSEASVDIIQSFVAPDAAGNIENNLKNEERRRNFKRESPR 120
           FQKEELKTKKIFVQHVETLRSSEASVDIIQSFVAPDAAGNIENNLKNEERRRNFKRESPR
Sbjct: 61  FQKEELKTKKIFVQHVETLRSSEASVDIIQSFVAPDAAGNIENNLKNEERRRNFKRESPR 120

Query: 121 QEQLLVKTRQEQEVLAKKARFEQIWRSRKGIKDPKDDQLRDAYHIYDIVRLDTNETSSEA 180
           QEQLLVKTRQEQEVLAKKARFEQIWRSRKGIKDP+DDQL DAYHIYDIVRLDTNETSSEA
Sbjct: 121 QEQLLVKTRQEQEVLAKKARFEQIWRSRKGIKDPRDDQLHDAYHIYDIVRLDTNETSSEA 180

Query: 181 PKAECMTLEDQSMLSSYLPLLREFIPSAAEEIESDINANTMKQDCKYHLIQQSLEVFCLQ 240
           PKAECMTLEDQSMLSSYLPLLREFIPSAAEEIESDINANTMKQD                
Sbjct: 181 PKAECMTLEDQSMLSSYLPLLREFIPSAAEEIESDINANTMKQD---------------- 240

Query: 241 LAVVTVISYCNVCVFTCSTVPVDDYVYDYYTVKNNVPIAEDDASNPFPLIQVDDLDLYDG 300
                              +PVDDYVYDYYTVKNNVPIAEDDASNPFPLIQVDDLDLYDG
Sbjct: 241 -------------------LPVDDYVYDYYTVKNNVPIAEDDASNPFPLIQVDDLDLYDG 300

Query: 301 PDDSDYESDDSNAENNPHFDYPDEASEEEELESESTNEESEEDDEDKQSSEANDLEEDDL 360
           PDDSDYESDDSNAENNPHFDYPDEASEEEELESESTNEESEEDDEDKQSSEAN LEEDDL
Sbjct: 301 PDDSDYESDDSNAENNPHFDYPDEASEEEELESESTNEESEEDDEDKQSSEANGLEEDDL 360

Query: 361 SENGVQLDDDEVYGDFDEDDGDSFDYDSNGGHGEGEDWRWSYR 404
           SENGVQLD+DE+YGDFDEDDGDSFDYDSNGG  EGEDWRWSYR
Sbjct: 361 SENGVQLDEDEIYGDFDEDDGDSFDYDSNGGQEEGEDWRWSYR 368

BLAST of Carg07027 vs. NCBI nr
Match: XP_022998602.1 (RNA-directed DNA methylation 4 [Cucurbita maxima])

HSP 1 Score: 629.4 bits (1622), Expect = 2.1e-176
Identity = 351/403 (87.10%), Postives = 357/403 (88.59%), Query Frame = 0

Query: 1   MASIGESSSSAPKFADEKPVLVRVKRKASQSRLDALWLEINERPLKRPLLDFDNLSISET 60
           MASIGESSSSAPK ADEKPVLVRVKRKASQSRLDALWLEINERPLKRPLLDFDNLSISET
Sbjct: 1   MASIGESSSSAPKPADEKPVLVRVKRKASQSRLDALWLEINERPLKRPLLDFDNLSISET 60

Query: 61  FQKEELKTKKIFVQHVETLRSSEASVDIIQSFVAPDAAGNIENNLKNEERRRNFKRESPR 120
           F+K ELKTKKIFVQHVETLRSSEASVDIIQSFVAPDAAGNIENNLKNEERRRNFKRESPR
Sbjct: 61  FKKAELKTKKIFVQHVETLRSSEASVDIIQSFVAPDAAGNIENNLKNEERRRNFKRESPR 120

Query: 121 QEQLLVKTRQEQEVLAKKARFEQIWRSRKGIKDPKDDQLRDAYHIYDIVRLDTNETSSEA 180
           QEQLLVKTRQEQEVL KKARFEQIWRSRKGIKD KDDQL DAYHIYDIVRLDTNETSS A
Sbjct: 121 QEQLLVKTRQEQEVLTKKARFEQIWRSRKGIKDAKDDQLHDAYHIYDIVRLDTNETSSGA 180

Query: 181 PKAECMTLEDQSMLSSYLPLLREFIPSAAEEIESDINANTMKQDCKYHLIQQSLEVFCLQ 240
           PKA+CMTLEDQSMLSSYLPLLREFIPSAAEEIESDINANTMKQD                
Sbjct: 181 PKADCMTLEDQSMLSSYLPLLREFIPSAAEEIESDINANTMKQD---------------- 240

Query: 241 LAVVTVISYCNVCVFTCSTVPVDDYVYDYYTVKNNVPIAEDDASNPFPLIQVDDLDLYDG 300
                              +PVDDYVYDYYTVKNNV IAEDDASNPFPLIQVDDLDLYDG
Sbjct: 241 -------------------LPVDDYVYDYYTVKNNVQIAEDDASNPFPLIQVDDLDLYDG 300

Query: 301 PDDSDYESDDSNAENNPHFDYPDEASEEEELESESTNEESEEDDEDKQSSEANDLEEDDL 360
           PDDSDYESDDSNAENNPHFDYPDEASEEEELESESTNEESEEDDEDKQSSEAN LEEDDL
Sbjct: 301 PDDSDYESDDSNAENNPHFDYPDEASEEEELESESTNEESEEDDEDKQSSEANGLEEDDL 360

Query: 361 SENGVQLDDDEVYGDFDEDDGDSFDYDSNGGHGEGEDWRWSYR 404
           SENGVQLD+DE+YGDFDE DGDSFDYDSNGGH EGE+ RWSYR
Sbjct: 361 SENGVQLDEDEIYGDFDEGDGDSFDYDSNGGHEEGEECRWSYR 368

BLAST of Carg07027 vs. ExPASy Swiss-Prot
Match: Q8GYP3 (RNA-directed DNA methylation 4 OS=Arabidopsis thaliana OX=3702 GN=RDM4 PE=1 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 4.2e-58
Identity = 163/385 (42.34%), Postives = 231/385 (60.00%), Query Frame = 0

Query: 1   MASIGESSSSAPKFADEKPVLVRVKRKASQSRLDALWLEINERPLKRPLLDFDNLSISET 60
           M  +GESS+      +EKPV+VRVKRK  QS LDA WLEINERPLKRP LDF  LSIS++
Sbjct: 1   MDGVGESSTQ--NEVEEKPVIVRVKRKVGQSLLDAFWLEINERPLKRPTLDFSKLSISDS 60

Query: 61  FQK-----EELKTKKIFVQHVETLRSSEASVDIIQSFVAPDAAGNIENNLKNEERRRNFK 120
            ++     E++K KK+ V+H+ET+  SE + DII SF   D      +  K EER+  FK
Sbjct: 61  GERGPSVAEDVKPKKVLVRHLETVTDSETTADIIHSFFESDHNEKSCSKGKFEERKIAFK 120

Query: 121 RESPRQEQLLVKTRQEQEVLAKKARFEQIWRSRKGIKDPKDDQLRDAYHIYDIVRLDTNE 180
           +++ R+EQ L K+ Q+Q++ ++ ARFEQIWRSRKG K    + + +  H +D++R+DT E
Sbjct: 121 KDN-RKEQRLTKSVQKQQIASENARFEQIWRSRKGNK----EGIHEKCHFFDVIRVDTEE 180

Query: 181 TSSEAPKAECMTLEDQSMLSSYLPLLREFIPSAAEEIESDINANTMKQDCKYHLIQQSLE 240
               A   E  +LEDQ ML+S+LPLLRE IP+AAEEIE+DI ++                
Sbjct: 181 RRDNA--QEFTSLEDQKMLASFLPLLRECIPTAAEEIEADIQSS---------------- 240

Query: 241 VFCLQLAVVTVISYCNVCVFTCSTVPVDDYVYDYYTVKNNVPIAEDDASNPFPLIQVDDL 300
                                      ++YVYDYY V   + I+ED + N FPL+ V+D 
Sbjct: 241 -------------------------HTEEYVYDYYAVNEEMDISEDSSKNQFPLVIVEDE 300

Query: 301 DLY-DGPDDSDYESDDSNAENNPHFDYPDEASEEEELESESTNEESEEDDEDKQSSEAND 360
           + + DG D+SDY+S+DSNAE++P  DYP+E  EEEE E +  +++ E ++E  ++S+ +D
Sbjct: 301 EEFCDGSDESDYDSEDSNAEDHPKTDYPEEEEEEEE-EDDDDDDDDESEEEKSEASDESD 333

Query: 361 LEEDDLSENGVQLDDDEVYGDFDED 380
            EE         L DDE + D+ ED
Sbjct: 361 DEETSKRHVRSVLGDDE-FDDYAED 333

BLAST of Carg07027 vs. ExPASy TrEMBL
Match: A0A6J1GCH8 (RNA-directed DNA methylation 4 OS=Cucurbita moschata OX=3662 GN=LOC111452902 PE=3 SV=1)

HSP 1 Score: 653.7 bits (1685), Expect = 5.1e-184
Identity = 360/403 (89.33%), Postives = 364/403 (90.32%), Query Frame = 0

Query: 1   MASIGESSSSAPKFADEKPVLVRVKRKASQSRLDALWLEINERPLKRPLLDFDNLSISET 60
           MASIGESSSSAPKFADEKPVLVRVKRKASQSRLDALWLEINERPLKRPLLDFDNLSISET
Sbjct: 1   MASIGESSSSAPKFADEKPVLVRVKRKASQSRLDALWLEINERPLKRPLLDFDNLSISET 60

Query: 61  FQKEELKTKKIFVQHVETLRSSEASVDIIQSFVAPDAAGNIENNLKNEERRRNFKRESPR 120
           FQKEELKTKKIFVQHVETLRSSEASVDIIQSFVAPDAAGNIENNLKNEERRRNFKRESPR
Sbjct: 61  FQKEELKTKKIFVQHVETLRSSEASVDIIQSFVAPDAAGNIENNLKNEERRRNFKRESPR 120

Query: 121 QEQLLVKTRQEQEVLAKKARFEQIWRSRKGIKDPKDDQLRDAYHIYDIVRLDTNETSSEA 180
           QEQLLVKTRQEQEVLAKKARFEQIWRSRKGIKDP+DDQL DAYHIYDIVRLDTNETSSEA
Sbjct: 121 QEQLLVKTRQEQEVLAKKARFEQIWRSRKGIKDPRDDQLHDAYHIYDIVRLDTNETSSEA 180

Query: 181 PKAECMTLEDQSMLSSYLPLLREFIPSAAEEIESDINANTMKQDCKYHLIQQSLEVFCLQ 240
           PKAECMTLEDQSMLSSYLPLLREFIPSAAEEIESDINANTMKQD                
Sbjct: 181 PKAECMTLEDQSMLSSYLPLLREFIPSAAEEIESDINANTMKQD---------------- 240

Query: 241 LAVVTVISYCNVCVFTCSTVPVDDYVYDYYTVKNNVPIAEDDASNPFPLIQVDDLDLYDG 300
                              +PVDDYVYDYYTVKNNVPIAEDDASNPFPLIQVDDLDLYDG
Sbjct: 241 -------------------LPVDDYVYDYYTVKNNVPIAEDDASNPFPLIQVDDLDLYDG 300

Query: 301 PDDSDYESDDSNAENNPHFDYPDEASEEEELESESTNEESEEDDEDKQSSEANDLEEDDL 360
           PDDSDYESDDSNAENNPHFDYPDEASEEEELESESTNEESEEDDEDKQSSEAN LEEDDL
Sbjct: 301 PDDSDYESDDSNAENNPHFDYPDEASEEEELESESTNEESEEDDEDKQSSEANGLEEDDL 360

Query: 361 SENGVQLDDDEVYGDFDEDDGDSFDYDSNGGHGEGEDWRWSYR 404
           SENGVQLD+DE+YGDFDEDDGDSFDYDSNGG  EGEDWRWSYR
Sbjct: 361 SENGVQLDEDEIYGDFDEDDGDSFDYDSNGGQEEGEDWRWSYR 368

BLAST of Carg07027 vs. ExPASy TrEMBL
Match: A0A6J1K8G0 (RNA-directed DNA methylation 4 OS=Cucurbita maxima OX=3661 GN=LOC111493190 PE=3 SV=1)

HSP 1 Score: 629.4 bits (1622), Expect = 1.0e-176
Identity = 351/403 (87.10%), Postives = 357/403 (88.59%), Query Frame = 0

Query: 1   MASIGESSSSAPKFADEKPVLVRVKRKASQSRLDALWLEINERPLKRPLLDFDNLSISET 60
           MASIGESSSSAPK ADEKPVLVRVKRKASQSRLDALWLEINERPLKRPLLDFDNLSISET
Sbjct: 1   MASIGESSSSAPKPADEKPVLVRVKRKASQSRLDALWLEINERPLKRPLLDFDNLSISET 60

Query: 61  FQKEELKTKKIFVQHVETLRSSEASVDIIQSFVAPDAAGNIENNLKNEERRRNFKRESPR 120
           F+K ELKTKKIFVQHVETLRSSEASVDIIQSFVAPDAAGNIENNLKNEERRRNFKRESPR
Sbjct: 61  FKKAELKTKKIFVQHVETLRSSEASVDIIQSFVAPDAAGNIENNLKNEERRRNFKRESPR 120

Query: 121 QEQLLVKTRQEQEVLAKKARFEQIWRSRKGIKDPKDDQLRDAYHIYDIVRLDTNETSSEA 180
           QEQLLVKTRQEQEVL KKARFEQIWRSRKGIKD KDDQL DAYHIYDIVRLDTNETSS A
Sbjct: 121 QEQLLVKTRQEQEVLTKKARFEQIWRSRKGIKDAKDDQLHDAYHIYDIVRLDTNETSSGA 180

Query: 181 PKAECMTLEDQSMLSSYLPLLREFIPSAAEEIESDINANTMKQDCKYHLIQQSLEVFCLQ 240
           PKA+CMTLEDQSMLSSYLPLLREFIPSAAEEIESDINANTMKQD                
Sbjct: 181 PKADCMTLEDQSMLSSYLPLLREFIPSAAEEIESDINANTMKQD---------------- 240

Query: 241 LAVVTVISYCNVCVFTCSTVPVDDYVYDYYTVKNNVPIAEDDASNPFPLIQVDDLDLYDG 300
                              +PVDDYVYDYYTVKNNV IAEDDASNPFPLIQVDDLDLYDG
Sbjct: 241 -------------------LPVDDYVYDYYTVKNNVQIAEDDASNPFPLIQVDDLDLYDG 300

Query: 301 PDDSDYESDDSNAENNPHFDYPDEASEEEELESESTNEESEEDDEDKQSSEANDLEEDDL 360
           PDDSDYESDDSNAENNPHFDYPDEASEEEELESESTNEESEEDDEDKQSSEAN LEEDDL
Sbjct: 301 PDDSDYESDDSNAENNPHFDYPDEASEEEELESESTNEESEEDDEDKQSSEANGLEEDDL 360

Query: 361 SENGVQLDDDEVYGDFDEDDGDSFDYDSNGGHGEGEDWRWSYR 404
           SENGVQLD+DE+YGDFDE DGDSFDYDSNGGH EGE+ RWSYR
Sbjct: 361 SENGVQLDEDEIYGDFDEGDGDSFDYDSNGGHEEGEECRWSYR 368

BLAST of Carg07027 vs. ExPASy TrEMBL
Match: A0A6J1DMY7 (RNA-directed DNA methylation 4 OS=Momordica charantia OX=3673 GN=LOC111022052 PE=3 SV=1)

HSP 1 Score: 543.5 bits (1399), Expect = 7.4e-151
Identity = 309/407 (75.92%), Postives = 337/407 (82.80%), Query Frame = 0

Query: 1   MASIGESSSSAPKFADEKPVLVRVKRKASQSRLDALWLEINERPLKRPLLDFDNLSISET 60
           MA+IGESSSS PK AD KPVLVRVKRKASQSRLDALWLEINERPLKRPLLDF+NLSISET
Sbjct: 1   MATIGESSSSVPKLADGKPVLVRVKRKASQSRLDALWLEINERPLKRPLLDFENLSISET 60

Query: 61  FQKEELKTKKIFVQHVETLRSSEASVDIIQSFVAPDAAGNIENNLKNEERRRNFKRESPR 120
           FQ EELKTKKIFVQHVETL+SSEA+VDI+QSFVAPDAAG +EN++KNEERRRNFKRE PR
Sbjct: 61  FQNEELKTKKIFVQHVETLKSSEATVDIVQSFVAPDAAGTVENSVKNEERRRNFKREIPR 120

Query: 121 QEQLLVKTRQEQEVLAKKARFEQIWRSRKGIKDPKDDQLRDAYHIYDIVRLDTNETSSEA 180
           Q+QLL K RQEQEVLAK ARFEQIWRSRKG+KD KDDQL D YHIYDIVRLDTN+ SSE 
Sbjct: 121 QDQLLSKARQEQEVLAKNARFEQIWRSRKGVKDAKDDQLHDMYHIYDIVRLDTNKISSEV 180

Query: 181 PKAECMTLEDQSMLSSYLPLLREFIPSAAEEIESDINANTMKQDCKYHLIQQSLEVFCLQ 240
           P+ E M+LEDQSMLSSYLPLLREFIPSAA EIESDI+AN MKQD                
Sbjct: 181 PEQEYMSLEDQSMLSSYLPLLREFIPSAAAEIESDIHANMMKQD---------------- 240

Query: 241 LAVVTVISYCNVCVFTCSTVPVDDYVYDYYTVKNNVPIAEDDASNPFPLIQVDDLDLYDG 300
                              VPVDDYVYDYYTVK+++ IAEDDASNPFPLIQVDDLDLYDG
Sbjct: 241 -------------------VPVDDYVYDYYTVKSDMEIAEDDASNPFPLIQVDDLDLYDG 300

Query: 301 PDDSDYESDDSNAENNPHFDYPDEASEE-EELESESTNEESEEDDED---KQSSEANDLE 360
           PDDSDYESDDSNAENNPHFDYPDE SEE +EL SES+N ES+++D+D   KQSSEAND E
Sbjct: 301 PDDSDYESDDSNAENNPHFDYPDEVSEEDDELGSESSNGESKDNDDDSEKKQSSEANDSE 360

Query: 361 EDDLSENGVQLDDDEVYGDFDEDDGDSFDYDSNGGHGEGEDWRWSYR 404
           EDDLS +  QL +DE+YGDFD+DD +SFD DSNGGH EGED RWSYR
Sbjct: 361 EDDLSVDRAQLYEDEIYGDFDDDDANSFDCDSNGGHDEGEDSRWSYR 372

BLAST of Carg07027 vs. ExPASy TrEMBL
Match: A0A0A0K5Q3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G238430 PE=4 SV=1)

HSP 1 Score: 519.2 bits (1336), Expect = 1.5e-143
Identity = 301/405 (74.32%), Postives = 329/405 (81.23%), Query Frame = 0

Query: 1   MASIGESSSSAPKFADEKPVLVRVKRKASQSRLDALWLEINERPLKRPLLDFDNLSISET 60
           MA IGESSSS  K  DEKPVLVRVKRKASQSRLDALWLEINERPLKRPLLDF+NLSISET
Sbjct: 1   MALIGESSSSVSKLVDEKPVLVRVKRKASQSRLDALWLEINERPLKRPLLDFENLSISET 60

Query: 61  FQKEELKTKKIFVQHVETLRSSEASVDIIQSFVAPDAAGNIENNLKNEERRRNFKRESPR 120
             KEELKTKKIFVQHVETLRSS+A+VDI+QSFVAPDAA  IENNLKNEERRRNFKRE  R
Sbjct: 61  LHKEELKTKKIFVQHVETLRSSDATVDIVQSFVAPDAASTIENNLKNEERRRNFKREISR 120

Query: 121 QEQLLVKTRQEQEVLAKKARFEQIWRSRKGIKDPKDDQLRDAYHIYDIVRLDTNETSSEA 180
           Q+QLLVK RQEQE+ AK ARFEQIWRSRKG+KD KDDQLRD YHIYDIVRLDTNE SSE+
Sbjct: 121 QDQLLVKARQEQELAAKNARFEQIWRSRKGVKDEKDDQLRDVYHIYDIVRLDTNEISSES 180

Query: 181 PKAECMTLEDQSMLSSYLPLLREFIPSAAEEIESDINANTMKQDCKYHLIQQSLEVFCLQ 240
           PK E M+LEDQSMLSSYLPLLREFIPSAA EIESDI+AN MKQ+                
Sbjct: 181 PKQEQMSLEDQSMLSSYLPLLREFIPSAAAEIESDIDANMMKQN---------------- 240

Query: 241 LAVVTVISYCNVCVFTCSTVPVDDYVYDYYTVKNNVPIAEDDASNPFPLIQVDDLDLYDG 300
                              +PVDDYVYDYYTVKN+V IAEDDAS+PFPLIQVDDLD +DG
Sbjct: 241 -------------------LPVDDYVYDYYTVKNDVEIAEDDASHPFPLIQVDDLD-HDG 300

Query: 301 PDDSDYESDDSNAENNPHFDYPDEASEEEELESESTNEESEEDDEDKQSSEANDLEEDDL 360
           P DSDYE+DDSNAENNP FDYPD    EEELES S+N+E E+ D++KQSSE+ND+EED+L
Sbjct: 301 PSDSDYETDDSNAENNPCFDYPD----EEELESTSSNDELEDSDDEKQSSESNDVEEDEL 360

Query: 361 S-ENGVQLDDDEVYGDFDEDDG-DSFDYDSNGGHGEGEDWRWSYR 404
           S E  V+L +DE+Y D DE+DG DSFDYDSN GH EGEDWRWSYR
Sbjct: 361 SEEEKVELYEDEIYDDCDEEDGADSFDYDSNVGHDEGEDWRWSYR 365

BLAST of Carg07027 vs. ExPASy TrEMBL
Match: A0A5D3BU38 (RNA-directed DNA methylation 4-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1161G00230 PE=4 SV=1)

HSP 1 Score: 518.5 bits (1334), Expect = 2.6e-143
Identity = 299/405 (73.83%), Postives = 328/405 (80.99%), Query Frame = 0

Query: 1   MASIGESSSSAPKFADEKPVLVRVKRKASQSRLDALWLEINERPLKRPLLDFDNLSISET 60
           MA IGESSSS PK  DEKPVLVRVKRKASQSRLDALWLEINERPLKRP LDF+NLSISET
Sbjct: 60  MALIGESSSSVPKLVDEKPVLVRVKRKASQSRLDALWLEINERPLKRPFLDFENLSISET 119

Query: 61  FQKEELKTKKIFVQHVETLRSSEASVDIIQSFVAPDAAGNIENNLKNEERRRNFKRESPR 120
             KEELKTKKIFVQHVETLRSSEA+ DI+QSFVAPDAA  IENNLKNEERRRNFKRE  R
Sbjct: 120 LYKEELKTKKIFVQHVETLRSSEATADIVQSFVAPDAASTIENNLKNEERRRNFKREISR 179

Query: 121 QEQLLVKTRQEQEVLAKKARFEQIWRSRKGIKDPKDDQLRDAYHIYDIVRLDTNETSSEA 180
           Q+QLLVK RQEQE++AK ARFEQIWRSRKG+KD KDDQL D YHIYDIVRLDTNE SSEA
Sbjct: 180 QDQLLVKARQEQELVAKNARFEQIWRSRKGVKDEKDDQLHDVYHIYDIVRLDTNEISSEA 239

Query: 181 PKAECMTLEDQSMLSSYLPLLREFIPSAAEEIESDINANTMKQDCKYHLIQQSLEVFCLQ 240
           P+ E M+LEDQSMLSSYLPLLREFIPSAA EIESDI+A+ MKQ+                
Sbjct: 240 PRQEQMSLEDQSMLSSYLPLLREFIPSAAAEIESDIDASMMKQN---------------- 299

Query: 241 LAVVTVISYCNVCVFTCSTVPVDDYVYDYYTVKNNVPIAEDDASNPFPLIQVDDLDLYDG 300
                              +PVDDYVYDYYTVK +V I EDDAS+PFPLIQVDDLD +DG
Sbjct: 300 -------------------LPVDDYVYDYYTVKTDVEIPEDDASHPFPLIQVDDLD-HDG 359

Query: 301 PDDSDYESDDSNAENNPHFDYPDEASEEEELESESTNEESEEDDEDKQSSEANDLEEDDL 360
           P DSDYE+DDSNAENNP FDYPD    EEELES+S+N+E E+ D++KQSSE+ND+EED+L
Sbjct: 360 PSDSDYETDDSNAENNPCFDYPD----EEELESKSSNDELEDSDDEKQSSESNDVEEDEL 419

Query: 361 S-ENGVQLDDDEVYGDFDEDDG-DSFDYDSNGGHGEGEDWRWSYR 404
           S E  V+L +DE+Y DFDEDDG DSFDYDSNGG  EGEDWRWSYR
Sbjct: 420 SEEEKVELYEDEIYDDFDEDDGADSFDYDSNGGRDEGEDWRWSYR 424

BLAST of Carg07027 vs. TAIR 10
Match: AT2G30280.1 (RNA-directed DNA methylation 4 )

HSP 1 Score: 226.9 bits (577), Expect = 3.0e-59
Identity = 163/385 (42.34%), Postives = 231/385 (60.00%), Query Frame = 0

Query: 1   MASIGESSSSAPKFADEKPVLVRVKRKASQSRLDALWLEINERPLKRPLLDFDNLSISET 60
           M  +GESS+      +EKPV+VRVKRK  QS LDA WLEINERPLKRP LDF  LSIS++
Sbjct: 1   MDGVGESSTQ--NEVEEKPVIVRVKRKVGQSLLDAFWLEINERPLKRPTLDFSKLSISDS 60

Query: 61  FQK-----EELKTKKIFVQHVETLRSSEASVDIIQSFVAPDAAGNIENNLKNEERRRNFK 120
            ++     E++K KK+ V+H+ET+  SE + DII SF   D      +  K EER+  FK
Sbjct: 61  GERGPSVAEDVKPKKVLVRHLETVTDSETTADIIHSFFESDHNEKSCSKGKFEERKIAFK 120

Query: 121 RESPRQEQLLVKTRQEQEVLAKKARFEQIWRSRKGIKDPKDDQLRDAYHIYDIVRLDTNE 180
           +++ R+EQ L K+ Q+Q++ ++ ARFEQIWRSRKG K    + + +  H +D++R+DT E
Sbjct: 121 KDN-RKEQRLTKSVQKQQIASENARFEQIWRSRKGNK----EGIHEKCHFFDVIRVDTEE 180

Query: 181 TSSEAPKAECMTLEDQSMLSSYLPLLREFIPSAAEEIESDINANTMKQDCKYHLIQQSLE 240
               A   E  +LEDQ ML+S+LPLLRE IP+AAEEIE+DI ++                
Sbjct: 181 RRDNA--QEFTSLEDQKMLASFLPLLRECIPTAAEEIEADIQSS---------------- 240

Query: 241 VFCLQLAVVTVISYCNVCVFTCSTVPVDDYVYDYYTVKNNVPIAEDDASNPFPLIQVDDL 300
                                      ++YVYDYY V   + I+ED + N FPL+ V+D 
Sbjct: 241 -------------------------HTEEYVYDYYAVNEEMDISEDSSKNQFPLVIVEDE 300

Query: 301 DLY-DGPDDSDYESDDSNAENNPHFDYPDEASEEEELESESTNEESEEDDEDKQSSEAND 360
           + + DG D+SDY+S+DSNAE++P  DYP+E  EEEE E +  +++ E ++E  ++S+ +D
Sbjct: 301 EEFCDGSDESDYDSEDSNAEDHPKTDYPEEEEEEEE-EDDDDDDDDESEEEKSEASDESD 333

Query: 361 LEEDDLSENGVQLDDDEVYGDFDED 380
            EE         L DDE + D+ ED
Sbjct: 361 DEETSKRHVRSVLGDDE-FDDYAED 333

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7037030.12.6e-214100.00RNA-directed DNA methylation 4 [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6607356.11.5e-18590.07RNA-directed DNA methylation 4, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023522069.11.2e-18489.33RNA-directed DNA methylation 4 [Cucurbita pepo subsp. pepo][more]
XP_022949593.11.1e-18389.33RNA-directed DNA methylation 4 [Cucurbita moschata][more]
XP_022998602.12.1e-17687.10RNA-directed DNA methylation 4 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q8GYP34.2e-5842.34RNA-directed DNA methylation 4 OS=Arabidopsis thaliana OX=3702 GN=RDM4 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1GCH85.1e-18489.33RNA-directed DNA methylation 4 OS=Cucurbita moschata OX=3662 GN=LOC111452902 PE=... [more]
A0A6J1K8G01.0e-17687.10RNA-directed DNA methylation 4 OS=Cucurbita maxima OX=3661 GN=LOC111493190 PE=3 ... [more]
A0A6J1DMY77.4e-15175.92RNA-directed DNA methylation 4 OS=Momordica charantia OX=3673 GN=LOC111022052 PE... [more]
A0A0A0K5Q31.5e-14374.32Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G238430 PE=4 SV=1[more]
A0A5D3BU382.6e-14373.83RNA-directed DNA methylation 4-like isoform X1 OS=Cucumis melo var. makuwa OX=11... [more]
Match NameE-valueIdentityDescription
AT2G30280.13.0e-5942.34RNA-directed DNA methylation 4 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 338..358
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 296..310
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 385..403
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 323..384
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 293..403
NoneNo IPR availablePANTHERPTHR31934ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 262..403
NoneNo IPR availablePANTHERPTHR31934:SF2RNA-DIRECTED DNA METHYLATION 4coord: 1..224
NoneNo IPR availablePANTHERPTHR31934:SF2RNA-DIRECTED DNA METHYLATION 4coord: 262..403
NoneNo IPR availablePANTHERPTHR31934ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 1..224
IPR013883Transcription factor Iwr1 domainPFAMPF08574Iwr1coord: 263..324
e-value: 1.7E-4
score: 22.5

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg07027-RACarg07027-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006306 DNA methylation
biological_process GO:0009791 post-embryonic development
biological_process GO:0006355 regulation of transcription, DNA-templated
molecular_function GO:0003700 DNA-binding transcription factor activity