Carg07021 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg07021
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Structure-specific endonuclease subunit SLX1 homolog
Location	Carg_Chr01: 3872522 .. 3873133 (-)
RNA-Seq Expression	Carg07021
Synteny	Carg07021

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGGAAGAGGAAAGAGAAACCGGATATCTCTGAAGCTACGGCGGTTGCAGAGGAGGAGGAGAAGGAAGACGATGAAGATGAAGGAAGCGGCAACAAATCGAACGGCTTCTTTTCATGCTATCTCTTGGCCTCTGTCTGCCCTTGTTTCAAAGGCCATACCTATATCGGGTTCGAATCCTTTCCTTCTTCTCGAACAAATTTTCTGCTTCTCTATGAAATAATGGCTTGAAATCCTCCATTTTCACGATTTCTTGATGCTCGAATCTGTTCTGATACTCCGATACTGGAAATTGTTTTACGATCTTTGAATCTGCAAATCTCTCGCTCTCTGCATAAATTGATCTCTGGATGCTCGAATCTGTTCCTCTACTCCCCGATACTGAAAATCGTTTTACGATCTTTGAATCTGTTCCTCTCTCGCTTTCTGCATAAATTGATTGAGGGAAAACATCAAGAAATATAGGAAATGTGTAAATCAATCTGCACTGAATTGATTGGATTACTGTTTATATTGAATTTCAGATTCACAGTGAACCCTAAACGCAGAATCAGGCAGCACAATGGTGAAATTAGATGCGGTACGTGGCGTACTAAGAGGAAGAGACCATGA

mRNA sequence

ATGAGGAAGAGGAAAGAGAAACCGGATATCTCTGAAGCTACGGCGGTTGCAGAGGAGGAGGAGAAGGAAGACGATGAAGATGAAGGAAGCGGCAACAAATCGAACGGCTTCTTTTCATGCTATCTCTTGGCCTCTGTCTGCCCTTGTTTCAAAGGCCATACCTATATCGGATTCACAGTGAACCCTAAACGCAGAATCAGGCAGCACAATGGTGAAATTAGATGCGGTACGTGGCGTACTAAGAGGAAGAGACCATGA

Coding sequence (CDS)

ATGAGGAAGAGGAAAGAGAAACCGGATATCTCTGAAGCTACGGCGGTTGCAGAGGAGGAGGAGAAGGAAGACGATGAAGATGAAGGAAGCGGCAACAAATCGAACGGCTTCTTTTCATGCTATCTCTTGGCCTCTGTCTGCCCTTGTTTCAAAGGCCATACCTATATCGGATTCACAGTGAACCCTAAACGCAGAATCAGGCAGCACAATGGTGAAATTAGATGCGGTACGTGGCGTACTAAGAGGAAGAGACCATGA

Protein sequence

MRKRKEKPDISEATAVAEEEEKEDDEDEGSGNKSNGFFSCYLLASVCPCFKGHTYIGFTVNPKRRIRQHNGEIRCGTWRTKRKRP

Homology

[Close]

BLAST of Carg07021 vs. NCBI nr
Match: KAG7037035.1 (Structure-specific endonuclease subunit SLX1-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 175.6 bits (444), Expect = 1.8e-40
Identity = 85/85 (100.00%), Postives = 85/85 (100.00%), Query Frame = 0


Query: 1  MRKRKEKPDISEATAVAEEEEKEDDEDEGSGNKSNGFFSCYLLASVCPCFKGHTYIGFTV 60
          MRKRKEKPDISEATAVAEEEEKEDDEDEGSGNKSNGFFSCYLLASVCPCFKGHTYIGFTV
Sbjct: 1  MRKRKEKPDISEATAVAEEEEKEDDEDEGSGNKSNGFFSCYLLASVCPCFKGHTYIGFTV 60

Query: 61 NPKRRIRQHNGEIRCGTWRTKRKRP 86
          NPKRRIRQHNGEIRCGTWRTKRKRP
Sbjct: 61 NPKRRIRQHNGEIRCGTWRTKRKRP 85

[Close]

BLAST of Carg07021 vs. NCBI nr
Match: KAG6607363.1 (Structure-specific endonuclease subunit SLX1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 153.3 bits (386), Expect = 9.4e-34
Identity = 74/75 (98.67%), Postives = 75/75 (100.00%), Query Frame = 0


Query: 1  MRKRKEKPDISEATAVAEEEEKEDDEDEGSGNKSNGFFSCYLLASVCPCFKGHTYIGFTV 60
          MRKRKEKP+ISEATAVAEEEEKEDDEDEGSGNKSNGFFSCYLLASVCPCFKGHTYIGFTV
Sbjct: 1  MRKRKEKPEISEATAVAEEEEKEDDEDEGSGNKSNGFFSCYLLASVCPCFKGHTYIGFTV 60

Query: 61 NPKRRIRQHNGEIRC 76
          NPKRRIRQHNGEIRC
Sbjct: 61 NPKRRIRQHNGEIRC 75

[Close]

BLAST of Carg07021 vs. NCBI nr
Match: KAG6583322.1 (Structure-specific endonuclease subunit SLX1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 141.0 bits (354), Expect = 4.8e-30
Identity = 69/85 (81.18%), Postives = 75/85 (88.24%), Query Frame = 0


Query: 1  MRKRKEKPDISEATAVAEEEEKEDDEDEGSGNKSNGFFSCYLLASVCPCFKGHTYIGFTV 60
          MR+RKEKP+I +  AVA EE+KEDDEDE  GN+ NGFFSCYLLAS CP +KGHTYIGFTV
Sbjct: 1  MRRRKEKPEIPKTMAVA-EEKKEDDEDEERGNEENGFFSCYLLASACPRYKGHTYIGFTV 60

Query: 61 NPKRRIRQHNGEIRCGTWRTKRKRP 86
          NPKRRIRQHNGEIRCG WRTKRKRP
Sbjct: 61 NPKRRIRQHNGEIRCGAWRTKRKRP 84

[Close]

BLAST of Carg07021 vs. NCBI nr
Match: XP_022970648.1 (uncharacterized protein LOC111469571 isoform X1 [Cucurbita maxima] >XP_022970649.1 uncharacterized protein LOC111469571 isoform X1 [Cucurbita maxima] >XP_022970650.1 uncharacterized protein LOC111469571 isoform X1 [Cucurbita maxima] >XP_022970651.1 uncharacterized protein LOC111469571 isoform X1 [Cucurbita maxima])

HSP 1 Score: 141.0 bits (354), Expect = 4.8e-30
Identity = 69/85 (81.18%), Postives = 75/85 (88.24%), Query Frame = 0


Query: 1  MRKRKEKPDISEATAVAEEEEKEDDEDEGSGNKSNGFFSCYLLASVCPCFKGHTYIGFTV 60
          MR+RKEKP+I +  AVA EE+KEDDEDE  GN+ NGFFSCYLLAS CP +KGHTYIGFTV
Sbjct: 1  MRRRKEKPEIPKTMAVA-EEKKEDDEDEERGNEGNGFFSCYLLASACPRYKGHTYIGFTV 60

Query: 61 NPKRRIRQHNGEIRCGTWRTKRKRP 86
          NPKRRIRQHNGEIRCG WRTKRKRP
Sbjct: 61 NPKRRIRQHNGEIRCGAWRTKRKRP 84

[Close]

BLAST of Carg07021 vs. NCBI nr
Match: XP_023519404.1 (uncharacterized protein LOC111782823 isoform X2 [Cucurbita pepo subsp. pepo] >XP_023519405.1 uncharacterized protein LOC111782823 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 141.0 bits (354), Expect = 4.8e-30
Identity = 69/85 (81.18%), Postives = 75/85 (88.24%), Query Frame = 0


Query: 1  MRKRKEKPDISEATAVAEEEEKEDDEDEGSGNKSNGFFSCYLLASVCPCFKGHTYIGFTV 60
          MR+RKEKP+I +  AVA EE+KEDDEDE  GN+ NGFFSCYLLAS CP +KGHTYIGFTV
Sbjct: 1  MRRRKEKPEIPKTMAVA-EEKKEDDEDEERGNEGNGFFSCYLLASACPRYKGHTYIGFTV 60

Query: 61 NPKRRIRQHNGEIRCGTWRTKRKRP 86
          NPKRRIRQHNGEIRCG WRTKRKRP
Sbjct: 61 NPKRRIRQHNGEIRCGAWRTKRKRP 84

[Close]

BLAST of Carg07021 vs. ExPASy Swiss-Prot
Match: A7STV9 (Structure-specific endonuclease subunit SLX1 homolog OS=Nematostella vectensis OX=45351 GN=v1g174298 PE=3 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 3.4e-10
Identity = 35/63 (55.56%), Postives = 42/63 (66.67%), Query Frame = 0


Query: 24 DDEDEGSGNKSNGFFSCYLLASVCPCFKGHTYIGFTVNPKRRIRQHNGEI-RCGTWRTKR 83
          DD   GS     GF   YLL  V P FKGHTYIG+TVNP RRI+QHNG + + G ++T R
Sbjct: 2  DDNIAGS-----GFHGVYLLYCVNPKFKGHTYIGYTVNPNRRIKQHNGGVDKGGAYKTSR 59

Query: 84 KRP 86
          K+P
Sbjct: 62 KKP 59

[Close]

BLAST of Carg07021 vs. ExPASy Swiss-Prot
Match: P0CN81 (Structure-specific endonuclease subunit SLX1 OS=Cryptococcus neoformans var. neoformans serotype D (strain B-3501A) OX=283643 GN=SLX1 PE=3 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 8.4e-09
Identity = 26/49 (53.06%), Postives = 32/49 (65.31%), Query Frame = 0


Query: 37 FFSCYLLASVCPCFKGHTYIGFTVNPKRRIRQHNGEIRCGTWRTKRKRP 86
          F++CYLL S        TY+G T +P RRIRQHNGE++ G W T R RP
Sbjct: 28 FYACYLLRSKATANSNRTYVGSTPDPPRRIRQHNGELKQGAWSTSRHRP 76

[Close]

BLAST of Carg07021 vs. ExPASy Swiss-Prot
Match: P0CN80 (Structure-specific endonuclease subunit SLX1 OS=Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) OX=214684 GN=SLX1 PE=3 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 8.4e-09
Identity = 26/49 (53.06%), Postives = 32/49 (65.31%), Query Frame = 0


Query: 37 FFSCYLLASVCPCFKGHTYIGFTVNPKRRIRQHNGEIRCGTWRTKRKRP 86
          F++CYLL S        TY+G T +P RRIRQHNGE++ G W T R RP
Sbjct: 28 FYACYLLRSKATANSNRTYVGSTPDPPRRIRQHNGELKQGAWSTSRHRP 76

[Close]

BLAST of Carg07021 vs. ExPASy Swiss-Prot
Match: Q4D7L5 (Structure-specific endonuclease subunit SLX1 homolog 1 OS=Trypanosoma cruzi (strain CL Brener) OX=353153 GN=Tc00.1047053509453.60 PE=3 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 1.1e-08
Identity = 28/47 (59.57%), Postives = 33/47 (70.21%), Query Frame = 0


Query: 37 FFSCYLLASVCPCFKGHTYIGFTVNPKRRIRQHNGEIRCGTWRTKRK 84
          F   YLL S+ P   G  YIG+TV+P RR+RQHNGEI  G WRTKR+
Sbjct: 5  FHCVYLLTSLDPQCAGEYYIGYTVDPIRRLRQHNGEIVSGAWRTKRR 51

[Close]

BLAST of Carg07021 vs. ExPASy Swiss-Prot
Match: Q4CTY5 (Structure-specific endonuclease subunit SLX1 homolog 2 OS=Trypanosoma cruzi (strain CL Brener) OX=353153 GN=Tc00.1047053511881.30 PE=3 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 1.1e-08
Identity = 28/47 (59.57%), Postives = 33/47 (70.21%), Query Frame = 0


Query: 37 FFSCYLLASVCPCFKGHTYIGFTVNPKRRIRQHNGEIRCGTWRTKRK 84
          F   YLL S+ P   G  YIG+TV+P RR+RQHNGEI  G WRTKR+
Sbjct: 5  FHCVYLLTSLDPQCAGEYYIGYTVDPIRRLRQHNGEIVSGAWRTKRR 51

[Close]

BLAST of Carg07021 vs. ExPASy TrEMBL
Match: A0A6J1I681 (Structure-specific endonuclease subunit SLX1 homolog OS=Cucurbita maxima OX=3661 GN=LOC111469571 PE=3 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 2.3e-30
Identity = 69/85 (81.18%), Postives = 75/85 (88.24%), Query Frame = 0


Query: 1  MRKRKEKPDISEATAVAEEEEKEDDEDEGSGNKSNGFFSCYLLASVCPCFKGHTYIGFTV 60
          MR+RKEKP+I +  AVA EE+KEDDEDE  GN+ NGFFSCYLLAS CP +KGHTYIGFTV
Sbjct: 1  MRRRKEKPEIPKTMAVA-EEKKEDDEDEERGNEGNGFFSCYLLASACPRYKGHTYIGFTV 60

Query: 61 NPKRRIRQHNGEIRCGTWRTKRKRP 86
          NPKRRIRQHNGEIRCG WRTKRKRP
Sbjct: 61 NPKRRIRQHNGEIRCGAWRTKRKRP 84

[Close]

BLAST of Carg07021 vs. ExPASy TrEMBL
Match: A0A6J1HZQ2 (Structure-specific endonuclease subunit SLX1 homolog OS=Cucurbita maxima OX=3661 GN=LOC111469571 PE=3 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 2.3e-30
Identity = 69/85 (81.18%), Postives = 75/85 (88.24%), Query Frame = 0


Query: 1  MRKRKEKPDISEATAVAEEEEKEDDEDEGSGNKSNGFFSCYLLASVCPCFKGHTYIGFTV 60
          MR+RKEKP+I +  AVA EE+KEDDEDE  GN+ NGFFSCYLLAS CP +KGHTYIGFTV
Sbjct: 1  MRRRKEKPEIPKTMAVA-EEKKEDDEDEERGNEGNGFFSCYLLASACPRYKGHTYIGFTV 60

Query: 61 NPKRRIRQHNGEIRCGTWRTKRKRP 86
          NPKRRIRQHNGEIRCG WRTKRKRP
Sbjct: 61 NPKRRIRQHNGEIRCGAWRTKRKRP 84

[Close]

BLAST of Carg07021 vs. ExPASy TrEMBL
Match: A0A6J1HKB9 (Structure-specific endonuclease subunit SLX1 homolog OS=Cucurbita moschata OX=3662 GN=LOC111464889 PE=3 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 2.0e-29
Identity = 68/85 (80.00%), Postives = 74/85 (87.06%), Query Frame = 0


Query: 1  MRKRKEKPDISEATAVAEEEEKEDDEDEGSGNKSNGFFSCYLLASVCPCFKGHTYIGFTV 60
          MR+RKEKP+I +  AVA EE+KEDDEDE  GN+ N FFSCYLLAS CP +KGHTYIGFTV
Sbjct: 1  MRRRKEKPEIPKTMAVA-EEKKEDDEDEERGNEGNRFFSCYLLASACPRYKGHTYIGFTV 60

Query: 61 NPKRRIRQHNGEIRCGTWRTKRKRP 86
          NPKRRIRQHNGEIRCG WRTKRKRP
Sbjct: 61 NPKRRIRQHNGEIRCGAWRTKRKRP 84

[Close]

BLAST of Carg07021 vs. ExPASy TrEMBL
Match: A0A6J1HMD6 (Structure-specific endonuclease subunit SLX1 homolog OS=Cucurbita moschata OX=3662 GN=LOC111464889 PE=3 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 2.0e-29
Identity = 68/85 (80.00%), Postives = 74/85 (87.06%), Query Frame = 0


Query: 1  MRKRKEKPDISEATAVAEEEEKEDDEDEGSGNKSNGFFSCYLLASVCPCFKGHTYIGFTV 60
          MR+RKEKP+I +  AVA EE+KEDDEDE  GN+ N FFSCYLLAS CP +KGHTYIGFTV
Sbjct: 1  MRRRKEKPEIPKTMAVA-EEKKEDDEDEERGNEGNRFFSCYLLASACPRYKGHTYIGFTV 60

Query: 61 NPKRRIRQHNGEIRCGTWRTKRKRP 86
          NPKRRIRQHNGEIRCG WRTKRKRP
Sbjct: 61 NPKRRIRQHNGEIRCGAWRTKRKRP 84

[Close]

BLAST of Carg07021 vs. ExPASy TrEMBL
Match: A0A0A0M0Y6 (Structure-specific endonuclease subunit SLX1 homolog OS=Cucumis sativus OX=3659 GN=Csa_1G533630 PE=3 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 3.4e-29
Identity = 68/85 (80.00%), Postives = 73/85 (85.88%), Query Frame = 0


Query: 1  MRKRKEKPDISEATAVAEEEEKEDDEDEGSGNKSNGFFSCYLLASVCPCFKGHTYIGFTV 60
          MRKRKEKP+I + T    +EEKEDDE+E  GN+ NGFFSCYLLAS CP FKGHTYIGFTV
Sbjct: 1  MRKRKEKPEICKTT----DEEKEDDEEEERGNEVNGFFSCYLLASACPRFKGHTYIGFTV 60

Query: 61 NPKRRIRQHNGEIRCGTWRTKRKRP 86
          NPKRRIRQHNGEIRCG WRTKRKRP
Sbjct: 61 NPKRRIRQHNGEIRCGAWRTKRKRP 81

[Close]

BLAST of Carg07021 vs. TAIR 10
Match: AT2G30350.2 (Excinuclease ABC, C subunit, N-terminal )

HSP 1 Score: 90.1 bits (222), Expect = 9.1e-19
Identity = 41/60 (68.33%), Postives = 46/60 (76.67%), Query Frame = 0


Query: 26 EDEGSGNKSNGFFSCYLLASVCPCFKGHTYIGFTVNPKRRIRQHNGEIRCGTWRTKRKRP 85
          ED  +G    GFF+CYLL S+ P  KG TYIGFTVNP+RRIRQHNGEI  G WRTK+KRP
Sbjct: 16 EDGVTGKDGKGFFACYLLTSLSPRHKGQTYIGFTVNPRRRIRQHNGEITSGAWRTKKKRP 75

The following BLAST results are available for this feature:

BLAST of Carg07021 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7037035.1	1.8e-40	100.00	Structure-specific endonuclease subunit SLX1-like protein, partial [Cucurbita ar...	[more]
KAG6607363.1	9.4e-34	98.67	Structure-specific endonuclease subunit SLX1, partial [Cucurbita argyrosperma su...	[more]
KAG6583322.1	4.8e-30	81.18	Structure-specific endonuclease subunit SLX1, partial [Cucurbita argyrosperma su...	[more]
XP_022970648.1	4.8e-30	81.18	uncharacterized protein LOC111469571 isoform X1 [Cucurbita maxima] >XP_022970649...	[more]
XP_023519404.1	4.8e-30	81.18	uncharacterized protein LOC111782823 isoform X2 [Cucurbita pepo subsp. pepo] >XP...	[more]

BLAST of Carg07021 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A7STV9	3.4e-10	55.56	Structure-specific endonuclease subunit SLX1 homolog OS=Nematostella vectensis O...	[more]
P0CN81	8.4e-09	53.06	Structure-specific endonuclease subunit SLX1 OS=Cryptococcus neoformans var. neo...	[more]
P0CN80	8.4e-09	53.06	Structure-specific endonuclease subunit SLX1 OS=Cryptococcus neoformans var. neo...	[more]
Q4D7L5	1.1e-08	59.57	Structure-specific endonuclease subunit SLX1 homolog 1 OS=Trypanosoma cruzi (str...	[more]
Q4CTY5	1.1e-08	59.57	Structure-specific endonuclease subunit SLX1 homolog 2 OS=Trypanosoma cruzi (str...	[more]

BLAST of Carg07021 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1I681	2.3e-30	81.18	Structure-specific endonuclease subunit SLX1 homolog OS=Cucurbita maxima OX=3661...	[more]
A0A6J1HZQ2	2.3e-30	81.18	Structure-specific endonuclease subunit SLX1 homolog OS=Cucurbita maxima OX=3661...	[more]
A0A6J1HKB9	2.0e-29	80.00	Structure-specific endonuclease subunit SLX1 homolog OS=Cucurbita moschata OX=36...	[more]
A0A6J1HMD6	2.0e-29	80.00	Structure-specific endonuclease subunit SLX1 homolog OS=Cucurbita moschata OX=36...	[more]
A0A0A0M0Y6	3.4e-29	80.00	Structure-specific endonuclease subunit SLX1 homolog OS=Cucumis sativus OX=3659 ...	[more]

BLAST of Carg07021 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G30350.2	9.1e-19	68.33	Excinuclease ABC, C subunit, N-terminal	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000305	GIY-YIG endonuclease	PFAM	PF01541	GIY-YIG	coord: 41..83 e-value: 1.3E-5 score: 25.4
IPR000305	GIY-YIG endonuclease	PROSITE	PS50164	GIY_YIG	coord: 36..85 score: 13.26993
IPR035901	GIY-YIG endonuclease superfamily	GENE3D	3.40.1440.10		coord: 33..85 e-value: 6.5E-11 score: 44.0
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..33
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..15
None	No IPR available	PANTHER	PTHR20208	STRUCTURE-SPECIFIC ENDONUCLEASE SUBUNIT SLX1	coord: 19..85
None	No IPR available	PANTHER	PTHR20208:SF10	STRUCTURE-SPECIFIC ENDONUCLEASE SUBUNIT SLX1	coord: 19..85

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg07021-RA	Carg07021-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0000724	double-strand break repair via homologous recombination
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
cellular_component	GO:0033557	Slx1-Slx4 complex
molecular_function	GO:0017108	5'-flap endonuclease activity
molecular_function	GO:0008821	crossover junction endodeoxyribonuclease activity