Carg06964 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg06964
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	NADH:cytochrome B5 reductase 1
Location	Carg_Chr20: 34752 .. 38067 (+)
RNA-Seq Expression	Carg06964
Synteny	Carg06964

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATATATTTCGAAGCTACGGACGGATGTGGGGTGGATGAGATCCCATTTTCGGCTGCATCCGCATCTCCCAACAACCGCAACCATTGAAGCCCTGTGATCCGATTCGTTCTCTTCCTGCAAATGGCGACGAGCTTCGTCCACTCTCACAGACCCGAGTTTCTCACCGTCGCCGTCGCTGCGGTTGCCATCGGCGTTGCTACTGCCTACTATTTCTATATCACCAAGAAATCTAAACCCCAAGGTTCTTATTCCTTCTCTTCCCTAATTCCTTCTACTCGGGCATTTTTGTTGTTTTGCTTGATTAAGATGCCGGGCTCCGGCTGTTGGATTTGGGAATACCTGGAACAAACCCTCCCCATCCCTATTTCATTTTTGCTGCTGATATCATCACTACCCTGCTGTTCGAGATGTTTCCGATTTTTACCGGGTTTTCTTTTTCTCTTTCTTATCTGTAGTTTCGTGTTATGCTAGGTTGCTTGGACCCTGATAACTTCAAAGAATTTAAGCTAGTTAAACGCACACAGCTTAGCCATAATGTAGCGAAGTTCAGATTCTCTCTTCCGACGCCAACCTCAATCTTGGGGCTGCCGATTGGGCAGCATATGAGTTGCAGGTTTTATTCGATTTGATACTTCGTGGAGTAAAGATTTATCAATGGAATGTTAGGAGTCTTCAAACGATTTGTTGTTGTAAGTTATGATGTGCTTTTGTTCAGAGGAAAAGATAGCGAAGGTGAAGAAGTTGTGAAACCGTACACTCCAACCAGTTTGGATACAGATGTTGGATACTTTGATTTGGTCATAAAGGTATAGGCCTTGCACTCCCAATGGGCAATAGCTTGCAGTTTTTCTTAGTGTGTTTAACCATCTGCCCAATTCTATCCATCCACCCATAACATTTGGTATTTTGGCATACAACTACTACAATATGCAGATGTATCCTCAAGGAAGGATGTCCCACCACTTTCGTGAGATGCGTGAAGGTGATTTCTTGGCGGTGAAAGGTCCTAAGGTATTGCCAATATGTCCTACAACTGCCACTACATTAGTAGAATTGAGATTTTCATTTTTTTCTCTGTTAATTTGAACCTTGAACGGCTAACTCGGTCAGAGTGGATGTTAGTCTTGAGTATACCTTTTATTTTGTCAAGTGTTCGCATTCTTTTTGAAAGGTTTATGAGTGGTTATGAGTATTCGTATGGCTGGACTGGGTGTGAATATTTTGTGGCTGCTAGTTACTAAGATGACGTTGGATGCTTTTTAGTTGTGTTTAAGCATTCTATTATTGAGTTTGAGGAGTTATTCTCTTTTGTTTGAATTGGTACGTTTACACAATTTTTTGGCTGGATTTTGGTTCTTTATTTGTTACTGTTGGGTGATTTATGGCTGGTTGAGTAATGTTTATTATTTATGTATATCGTTTGAGGAGGCATTGTTTTAACCACTCATCAAGTTTTAGAGTTTGAATTGGACGGAATAAAAAATTGAAGGCAAAGATTGACATAAGTTAAGGAAAAAAATCAGTCCCTTTGGGGGATTGGGATTCATAATAAAACATAAGGTTATATTAGAACTTCGAGGAGCGCATTGAACAGCCCAACTGTTTCTGGTCTCAAATTTTCTGTTTTGGAGGAGCGAGGTACTTAAAATATTAGATTTTTGACATTTGATGTGGGAAAATCTAAAACTGGGGCACGGGTTCTGGGGTCATATCTATTATTTATGGAGTGGGTGTCTGTTTGTGTGCGCGAGTGGTGACAGGGACGTTTCAAGTATGAAGCTAATGAAGTTGAGGCCTTTGGGATGCTTGCTGGAGGCACAGGCATAACTCCGATGTTCCAGGTATGGGTATAGGCATGGGCATGGGCTAAGTTTGAGCTTAAGTTTTGAGTAAGGATTGATTCGTATTTATTTTCTGTTTGTCCTATAAATAAGCAGATCCCATCAGCATTTCTGAACGTTTCTTTTTTGTGTTTTGAGGTTGCACGAGCAATACTAGAAAACCCAGTTGATCACACCAATGTGCATCTTATATATGCTAATGCTACTTATGAAGACATTCTTCTAAAGGTGCTCATTCTCCTCTCTTAAATTTGGATTTGGAAGGTACCCCACATTGAGATTTAGATATTGCCTTGCAAATCCGAACTGAAACCTTCTAGTTTACTGGTGTTTATAGGAAGAATTGGATAGTCTTGTGTCTAGGTTCCCCTCCCGCTTTTCTGTCTACTATGTGCTCGATCAGGTAACAACTTGGGGTTTTATAATTTTGGAAATATATTCTGCAGTTTCATGTCGTTTTACAATCTCTCTCTGCTCCGTACAGCCTCCTGAAGGATGGGATGGCGGCGTTGGCTTTGTGACTGAGGAAATGATACGACTACACTGTCCTGGACCATCCCCAAATGTTCGGGTGGGTATGCATGCTTTGCTTCATTCGTTTTTCTTTCTCTCTGTAAGAGAAAGTTTGTGCTAAAAACAGGCATTGTTGTTCATTTTGCGACAGATATTGAGGTGCGGACCACCCCCCATGAACAAAGCTATGGCTGCTTACCTCAAAACTATTGGCTATACTTCCCAAATGCAGTTCCAGTTCTAATCCAACCAACCCCATTATTTACTCTCCTCTTTGCCGATCTAATTCAATCTTCCCATCCCCATCCCAAAAGCAATTGAAGTAAATGTGTATCCAACAAACAATGATATGTTAAACCCAATATTTGGTATTGAACTTGATGTGATATGCAGACATACAGCTACTGGGTTCACATTAAAGAAAAGGTAAGGCAAGGAAAGAAGTTACAGAAAAGCAGCAATGCTGTCGCTCTCTCTGCTACCAACAATCCCACACTTTATGAGGCTCCCAATGGTGTTCCACATCAACTTTGCATCTTCCACTCTGTTTCCCTCTGCCAATTTCTTGTATAGATAGCTTTCGAAAGTCATTCTCTGCACATACTCGTTCCCACACATCTCCACCAATGCTTCCCTTCCGGCGTTGTCTCCATAGAACACTCTCTGCAACAGATCCAAGAACCTGAACGTCCCCACGTACTTGGCGTCCCACTCTCTCAGGTACTCTCTCTTCAGATCCCTTTCTCCGATCATCCTCTCCCCTCCTTCCGATGCTCGCACCACTGCCTCCCCGCACATTCTCCCGGATTTTGCAGCGAAATATATGCCTTCCCCGGAGCACTTCGTTACGTACCCTGCCGCGTCCCCGACCAGTGCCACCCGCCCTATCACTCGCCTAGCTCGCGGGTGCTCCGGTATTGGATGTGCCTCTACTTTAATCACCTTCCCTCCACTTATCTTAC

mRNA sequence

ATATATTTCGAAGCTACGGACGGATGTGGGGTGGATGAGATCCCATTTTCGGCTGCATCCGCATCTCCCAACAACCGCAACCATTGAAGCCCTGTGATCCGATTCGTTCTCTTCCTGCAAATGGCGACGAGCTTCGTCCACTCTCACAGACCCGAGTTTCTCACCGTCGCCGTCGCTGCGGTTGCCATCGGCGTTGCTACTGCCTACTATTTCTATATCACCAAGAAATCTAAACCCCAAGGTTGCTTGGACCCTGATAACTTCAAAGAATTTAAGCTAGTTAAACGCACACAGCTTAGCCATAATGTAGCGAAGTTCAGATTCTCTCTTCCGACGCCAACCTCAATCTTGGGGCTGCCGATTGGGCAGCATATGAGTTGCAGAGGAAAAGATAGCGAAGGTGAAGAAGTTGTGAAACCGTACACTCCAACCAGTTTGGATACAGATGTTGGATACTTTGATTTGGTCATAAAGATGTATCCTCAAGGAAGGATGTCCCACCACTTTCGTGAGATGCGTGAAGGTGATTTCTTGGCGGTGAAAGGTCCTAAGGGACGTTTCAAGTATGAAGCTAATGAAGTTGAGGCCTTTGGGATGCTTGCTGGAGGCACAGGCATAACTCCGATGTTCCAGGTTGCACGAGCAATACTAGAAAACCCAGTTGATCACACCAATGTGCATCTTATATATGCTAATGCTACTTATGAAGACATTCTTCTAAAGGAAGAATTGGATAGTCTTGTGTCTAGGTTCCCCTCCCGCTTTTCTGTCTACTATGTGCTCGATCAGCCTCCTGAAGGATGGGATGGCGGCGTTGGCTTTGTGACTGAGGAAATGATACGACTACACTGTCCTGGACCATCCCCAAATGTTCGGGTGGATATTGAGGTGCGGACCACCCCCCATGAACAAAGCTATGGCTGCTTACCTCAAAACTATTGGCTATACTTCCCAAATGCAGTTCCAGTTCTAATCCAACCAACCCCATTATTTACTCTCCTCTTTGCCGATCTAATTCAATCTTCCCATCCCCATCCCAAAAGCAATTGAAGTAAATGTGTATCCAACAAACAATGATATGTTAAACCCAATATTTGGTATTGAACTTGATGTGATATGCAGACATACAGCTACTGGGTTCACATTAAAGAAAAGGTAAGGCAAGGAAAGAAGTTACAGAAAAGCAGCAATGCTGTCGCTCTCTCTGCTACCAACAATCCCACACTTTATGAGGCTCCCAATGGTGTTCCACATCAACTTTGCATCTTCCACTCTGTTTCCCTCTGCCAATTTCTTGTATAGATAGCTTTCGAAAGTCATTCTCTGCACATACTCGTTCCCACACATCTCCACCAATGCTTCCCTTCCGGCGTTGTCTCCATAGAACACTCTCTGCAACAGATCCAAGAACCTGAACGTCCCCACGTACTTGGCGTCCCACTCTCTCAGGTACTCTCTCTTCAGATCCCTTTCTCCGATCATCCTCTCCCCTCCTTCCGATGCTCGCACCACTGCCTCCCCGCACATTCTCCCGGATTTTGCAGCGAAATATATGCCTTCCCCGGAGCACTTCGTTACGTACCCTGCCGCGTCCCCGACCAGTGCCACCCGCCCTATCACTCGCCTAGCTCGCGGGTGCTCCGGTATTGGATGTGCCTCTACTTTAATCACCTTCCCTCCACTTATCTTAC

Coding sequence (CDS)

ATGGCGACGAGCTTCGTCCACTCTCACAGACCCGAGTTTCTCACCGTCGCCGTCGCTGCGGTTGCCATCGGCGTTGCTACTGCCTACTATTTCTATATCACCAAGAAATCTAAACCCCAAGGTTGCTTGGACCCTGATAACTTCAAAGAATTTAAGCTAGTTAAACGCACACAGCTTAGCCATAATGTAGCGAAGTTCAGATTCTCTCTTCCGACGCCAACCTCAATCTTGGGGCTGCCGATTGGGCAGCATATGAGTTGCAGAGGAAAAGATAGCGAAGGTGAAGAAGTTGTGAAACCGTACACTCCAACCAGTTTGGATACAGATGTTGGATACTTTGATTTGGTCATAAAGATGTATCCTCAAGGAAGGATGTCCCACCACTTTCGTGAGATGCGTGAAGGTGATTTCTTGGCGGTGAAAGGTCCTAAGGGACGTTTCAAGTATGAAGCTAATGAAGTTGAGGCCTTTGGGATGCTTGCTGGAGGCACAGGCATAACTCCGATGTTCCAGGTTGCACGAGCAATACTAGAAAACCCAGTTGATCACACCAATGTGCATCTTATATATGCTAATGCTACTTATGAAGACATTCTTCTAAAGGAAGAATTGGATAGTCTTGTGTCTAGGTTCCCCTCCCGCTTTTCTGTCTACTATGTGCTCGATCAGCCTCCTGAAGGATGGGATGGCGGCGTTGGCTTTGTGACTGAGGAAATGATACGACTACACTGTCCTGGACCATCCCCAAATGTTCGGGTGGATATTGAGGTGCGGACCACCCCCCATGAACAAAGCTATGGCTGCTTACCTCAAAACTATTGGCTATACTTCCCAAATGCAGTTCCAGTTCTAATCCAACCAACCCCATTATTTACTCTCCTCTTTGCCGATCTAATTCAATCTTCCCATCCCCATCCCAAAAGCAATTGA

Protein sequence

MATSFVHSHRPEFLTVAVAAVAIGVATAYYFYITKKSKPQGCLDPDNFKEFKLVKRTQLSHNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMYPQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENPVDHTNVHLIYANATYEDILLKEELDSLVSRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMIRLHCPGPSPNVRVDIEVRTTPHEQSYGCLPQNYWLYFPNAVPVLIQPTPLFTLLFADLIQSSHPHPKSN

Homology

[Close]

BLAST of Carg06964 vs. NCBI nr
Match: KAG7010210.1 (NADH--cytochrome b5 reductase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 646.4 bits (1666), Expect = 1.3e-181
Identity = 309/309 (100.00%), Postives = 309/309 (100.00%), Query Frame = 0


Query: 1   MATSFVHSHRPEFLTVAVAAVAIGVATAYYFYITKKSKPQGCLDPDNFKEFKLVKRTQLS 60
           MATSFVHSHRPEFLTVAVAAVAIGVATAYYFYITKKSKPQGCLDPDNFKEFKLVKRTQLS
Sbjct: 1   MATSFVHSHRPEFLTVAVAAVAIGVATAYYFYITKKSKPQGCLDPDNFKEFKLVKRTQLS 60

Query: 61  HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMY 120
           HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMY
Sbjct: 61  HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMY 120

Query: 121 PQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENP 180
           PQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENP
Sbjct: 121 PQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENP 180

Query: 181 VDHTNVHLIYANATYEDILLKEELDSLVSRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMI 240
           VDHTNVHLIYANATYEDILLKEELDSLVSRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMI
Sbjct: 181 VDHTNVHLIYANATYEDILLKEELDSLVSRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMI 240

Query: 241 RLHCPGPSPNVRVDIEVRTTPHEQSYGCLPQNYWLYFPNAVPVLIQPTPLFTLLFADLIQ 300
           RLHCPGPSPNVRVDIEVRTTPHEQSYGCLPQNYWLYFPNAVPVLIQPTPLFTLLFADLIQ
Sbjct: 241 RLHCPGPSPNVRVDIEVRTTPHEQSYGCLPQNYWLYFPNAVPVLIQPTPLFTLLFADLIQ 300

Query: 301 SSHPHPKSN 310
           SSHPHPKSN
Sbjct: 301 SSHPHPKSN 309

[Close]

BLAST of Carg06964 vs. NCBI nr
Match: XP_022944291.1 (NADH--cytochrome b5 reductase 1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 617.1 bits (1590), Expect = 8.4e-173
Identity = 297/306 (97.06%), Postives = 299/306 (97.71%), Query Frame = 0


Query: 1   MATSFVHSHRPEFLTVAVAAVAIGVATAYYFYITKKSKPQGCLDPDNFKEFKLVKRTQLS 60
           MATSFVHSHRPEFLTVAVAAVAIGVATAYYFYITKKSKPQGCLDPDNFKEFKLVKR QLS
Sbjct: 1   MATSFVHSHRPEFLTVAVAAVAIGVATAYYFYITKKSKPQGCLDPDNFKEFKLVKRKQLS 60

Query: 61  HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMY 120
           HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMY
Sbjct: 61  HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMY 120

Query: 121 PQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENP 180
           PQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENP
Sbjct: 121 PQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENP 180

Query: 181 VDHTNVHLIYANATYEDILLKEELDSLVSRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMI 240
           VDHTNVHLIYANATYEDILLKEELDSL SRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMI
Sbjct: 181 VDHTNVHLIYANATYEDILLKEELDSLASRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMI 240

Query: 241 RLHCPGPSPNVRVDIEVRTTPHEQSYGCLPQNYWLYFPNAVPVLIQPTPLFTLLFADLIQ 300
           RLHCPGPSPNVRVDIEVRTTPHEQSYGCLPQNYWLYFPNAVPVLIQPTPLFTLLFA L +
Sbjct: 241 RLHCPGPSPNVRVDIEVRTTPHEQSYGCLPQNYWLYFPNAVPVLIQPTPLFTLLFALLAR 300

Query: 301 SSHPHP 307
           S+   P
Sbjct: 301 SNSIFP 306

[Close]

BLAST of Carg06964 vs. NCBI nr
Match: XP_022985759.1 (NADH--cytochrome b5 reductase 1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 611.7 bits (1576), Expect = 3.5e-171
Identity = 294/306 (96.08%), Postives = 296/306 (96.73%), Query Frame = 0


Query: 1   MATSFVHSHRPEFLTVAVAAVAIGVATAYYFYITKKSKPQGCLDPDNFKEFKLVKRTQLS 60
           MATSFVHSHRPEFLTVAVAAVAIGVAT YYFYITKKSKPQGCLDPD FKEFKLVKRTQLS
Sbjct: 1   MATSFVHSHRPEFLTVAVAAVAIGVATTYYFYITKKSKPQGCLDPDKFKEFKLVKRTQLS 60

Query: 61  HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMY 120
           HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMY
Sbjct: 61  HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMY 120

Query: 121 PQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENP 180
           PQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENP
Sbjct: 121 PQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENP 180

Query: 181 VDHTNVHLIYANATYEDILLKEELDSLVSRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMI 240
           VDHTN HLIYANATYEDILLKEELDSL SRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMI
Sbjct: 181 VDHTNAHLIYANATYEDILLKEELDSLASRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMI 240

Query: 241 RLHCPGPSPNVRVDIEVRTTPHEQSYGCLPQNYWLYFPNAVPVLIQPTPLFTLLFADLIQ 300
           RLHCPGPSPNVRVDIEVRTTPHEQSYGCLPQNYWLY PNAVPVLIQPTPLFTLLFA L +
Sbjct: 241 RLHCPGPSPNVRVDIEVRTTPHEQSYGCLPQNYWLYLPNAVPVLIQPTPLFTLLFALLAR 300

Query: 301 SSHPHP 307
           S+   P
Sbjct: 301 SNSIFP 306

[Close]

BLAST of Carg06964 vs. NCBI nr
Match: XP_022944292.1 (NADH--cytochrome b5 reductase 1-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 562.4 bits (1448), Expect = 2.5e-156
Identity = 276/306 (90.20%), Postives = 278/306 (90.85%), Query Frame = 0


Query: 1   MATSFVHSHRPEFLTVAVAAVAIGVATAYYFYITKKSKPQGCLDPDNFKEFKLVKRTQLS 60
           MATSFVHSHRPEFLTVAVAAVAIGVATAYYFYITKKSKPQGCLDPDNFKEFKLVKR QLS
Sbjct: 1   MATSFVHSHRPEFLTVAVAAVAIGVATAYYFYITKKSKPQGCLDPDNFKEFKLVKRKQLS 60

Query: 61  HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMY 120
           HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMY
Sbjct: 61  HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMY 120

Query: 121 PQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENP 180
           PQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENP
Sbjct: 121 PQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENP 180

Query: 181 VDHTNVHLIYANATYEDILLKEELDSLVSRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMI 240
           VDHTNVHLIYANATYEDILLK                      PPEGWDGGVGFVTEEMI
Sbjct: 181 VDHTNVHLIYANATYEDILLK----------------------PPEGWDGGVGFVTEEMI 240

Query: 241 RLHCPGPSPNVRVDIEVRTTPHEQSYGCLPQNYWLYFPNAVPVLIQPTPLFTLLFADLIQ 300
           RLHCPGPSPNVRVDIEVRTTPHEQSYGCLPQNYWLYFPNAVPVLIQPTPLFTLLFA L +
Sbjct: 241 RLHCPGPSPNVRVDIEVRTTPHEQSYGCLPQNYWLYFPNAVPVLIQPTPLFTLLFALLAR 284

Query: 301 SSHPHP 307
           S+   P
Sbjct: 301 SNSIFP 284

[Close]

BLAST of Carg06964 vs. NCBI nr
Match: XP_022985760.1 (NADH--cytochrome b5 reductase 1-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 557.0 bits (1434), Expect = 1.0e-154
Identity = 273/306 (89.22%), Postives = 275/306 (89.87%), Query Frame = 0


Query: 1   MATSFVHSHRPEFLTVAVAAVAIGVATAYYFYITKKSKPQGCLDPDNFKEFKLVKRTQLS 60
           MATSFVHSHRPEFLTVAVAAVAIGVAT YYFYITKKSKPQGCLDPD FKEFKLVKRTQLS
Sbjct: 1   MATSFVHSHRPEFLTVAVAAVAIGVATTYYFYITKKSKPQGCLDPDKFKEFKLVKRTQLS 60

Query: 61  HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMY 120
           HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMY
Sbjct: 61  HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMY 120

Query: 121 PQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENP 180
           PQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENP
Sbjct: 121 PQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENP 180

Query: 181 VDHTNVHLIYANATYEDILLKEELDSLVSRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMI 240
           VDHTN HLIYANATYEDILLK                      PPEGWDGGVGFVTEEMI
Sbjct: 181 VDHTNAHLIYANATYEDILLK----------------------PPEGWDGGVGFVTEEMI 240

Query: 241 RLHCPGPSPNVRVDIEVRTTPHEQSYGCLPQNYWLYFPNAVPVLIQPTPLFTLLFADLIQ 300
           RLHCPGPSPNVRVDIEVRTTPHEQSYGCLPQNYWLY PNAVPVLIQPTPLFTLLFA L +
Sbjct: 241 RLHCPGPSPNVRVDIEVRTTPHEQSYGCLPQNYWLYLPNAVPVLIQPTPLFTLLFALLAR 284

Query: 301 SSHPHP 307
           S+   P
Sbjct: 301 SNSIFP 284

[Close]

BLAST of Carg06964 vs. ExPASy Swiss-Prot
Match: Q9ZNT1 (NADH--cytochrome b5 reductase 1 OS=Arabidopsis thaliana OX=3702 GN=CBR1 PE=1 SV=1)

HSP 1 Score: 369.8 bits (948), Expect = 3.1e-101
Identity = 174/254 (68.50%), Postives = 208/254 (81.89%), Query Frame = 0


Query: 1   MATSFVHS-HRPEFLTVAVAAVAIGVATAYYFYITKKSKPQGCLDPDNFKEFKLVKRTQL 60
           M T F+ +  R   L V VA VA+G   AY  ++T   K + CLDP+NFKEFKLVKR QL
Sbjct: 1   MDTEFLRTLDRQILLGVFVAFVAVGAGAAY--FLTSSKKRRVCLDPENFKEFKLVKRHQL 60

Query: 61  SHNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKM 120
           SHNVAKF F LPT TS+LGLPIGQH+SCRGKD +GE+V+KPYTPT+LD+DVG F+LVIKM
Sbjct: 61  SHNVAKFVFELPTSTSVLGLPIGQHISCRGKDGQGEDVIKPYTPTTLDSDVGRFELVIKM 120

Query: 121 YPQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILEN 180
           YPQGRMSHHFREMR GD LAVKGPKGRFKY+  +  AFGMLAGG+GITPMFQVARAILEN
Sbjct: 121 YPQGRMSHHFREMRVGDHLAVKGPKGRFKYQPGQFRAFGMLAGGSGITPMFQVARAILEN 180

Query: 181 PVDHTNVHLIYANATYEDILLKEELDSLVSRFPSRFSVYYVLDQPPEGWDGGVGFVTEEM 240
           P D T VHLIYAN TY+DILLKEEL+ L + +P +F ++YVL+QPPE WDGGVGFV++EM
Sbjct: 181 PTDKTKVHLIYANVTYDDILLKEELEGLTTNYPEQFKIFYVLNQPPEVWDGGVGFVSKEM 240

Query: 241 IRLHCPGPSPNVRV 254
           I+ HCP P+ ++++
Sbjct: 241 IQTHCPAPASDIQI 252

[Close]

BLAST of Carg06964 vs. ExPASy Swiss-Prot
Match: Q6BUX2 (NADH-cytochrome b5 reductase 1 OS=Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968) OX=284592 GN=CBR1 PE=3 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 1.5e-63
Identity = 118/237 (49.79%), Postives = 159/237 (67.09%), Query Frame = 0


Query: 13  FLTVAVAAVAIGVATAYYFY-ITKKSKPQGCLDPDNFKEFKLVKRTQLSHNVAKFRFSLP 72
           F+  A  A  I  A AYYF+ +++K+ P   L P++F++F L+++T++SHN   +RF LP
Sbjct: 9   FIIFATVAAIISSAVAYYFFQLSRKNAP--VLKPNDFQKFPLIEKTRVSHNTCVYRFGLP 68

Query: 73  TPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMYPQGRMSHHFRE 132
             T  LGLPIGQH++  G     +EVV+ YTP S D ++GYFDL+IK Y  G +S H   
Sbjct: 69  RSTDRLGLPIGQHIAI-GATINDKEVVRSYTPISTDDELGYFDLLIKAYENGNISRHVES 128

Query: 133 MREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENPVDHTNVHLIYA 192
            + G+ + ++GPKG F Y    VE+FGM+AGGTGITPM+Q+  AIL NP D T V L+YA
Sbjct: 129 KKIGETIDIRGPKGFFTYTPGMVESFGMIAGGTGITPMYQILTAILRNPEDKTKVSLVYA 188

Query: 193 NATYEDILLKEELDSLVSRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMIRLHCPGPS 249
           N T +DILLKEEL+ +    P RF +YYVL+ PPE W GGVGFVT E++  H P  S
Sbjct: 189 NVTEDDILLKEELNKMAREHPDRFKIYYVLNTPPENWTGGVGFVTPEIMDKHLPKAS 242

[Close]

BLAST of Carg06964 vs. ExPASy Swiss-Prot
Match: Q6CA86 (NADH-cytochrome b5 reductase 1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=CBR1 PE=3 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 2.8e-62
Identity = 119/244 (48.77%), Postives = 164/244 (67.21%), Query Frame = 0


Query: 13  FLTVAVAAVAIGVATAYYFYITKKSKPQGCLDPDN--FKEFKLVKRTQLSHNVAKFRFSL 72
           +    V A+ IG AT Y+F     SKP+  L   +  FKEF L+++T LSHN A +RF L
Sbjct: 15  YAIATVWALIIGAATYYFF---SNSKPKAVLQRGDTAFKEFPLIQKTVLSHNSAIYRFGL 74

Query: 73  PTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDT-DVGYFDLVIKMYPQGRMSHHF 132
           P P+ +LGLPIGQH+S    +  G+EV++ YTPTS D  D GYFD++IK YPQG +S + 
Sbjct: 75  PRPSHVLGLPIGQHVSL-SANIGGKEVLRSYTPTSSDLYDKGYFDILIKTYPQGNISKYV 134

Query: 133 REMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENPVDHTNVHLI 192
            E+  GD + V+GPKG F Y    VE+FGM+ GGTGITPM+Q+ R I  +P D+T V+L+
Sbjct: 135 SELAIGDTMKVRGPKGNFVYNHGLVESFGMVCGGTGITPMYQILRHIAADPADNTKVNLV 194

Query: 193 YANATYEDILLKEELDSLVSRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMIRLHCPGPSP 252
           YAN  ++DILLK+ELD++ +       ++YVL+  PE W G VGFVT+E++  HCP P P
Sbjct: 195 YANVNHDDILLKKELDAIAAE-NDNIKIHYVLNNAPEDWTGSVGFVTKEILEKHCPPPGP 253

Query: 253 NVRV 254
           N ++
Sbjct: 255 NTKL 253

[Close]

BLAST of Carg06964 vs. ExPASy Swiss-Prot
Match: A1C7E9 (NADH-cytochrome b5 reductase 1 OS=Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1) OX=344612 GN=cbr1 PE=3 SV=1)

HSP 1 Score: 238.8 bits (608), Expect = 8.1e-62
Identity = 123/250 (49.20%), Postives = 162/250 (64.80%), Query Frame = 0


Query: 10  RPEFLTVAVAAVAIGVATAYYFYITKKSKPQGCLDPDNFKEFKLVKRTQLSHNVAKFRFS 69
           + EF+  AVA  A+    A +   T  SK +  L+P  F+EF L ++T +SHNV+ +RF+
Sbjct: 27  KKEFVPYAVALTAV---LAGFKLFTGDSKARKVLNPTEFQEFVLKEKTDISHNVSIYRFA 86

Query: 70  LPTPTSILGLPIGQHMSCRGK-DSEGEEVVKPYTPTSLDTDVGYFDLVIKMYPQGRMSHH 129
           LP PT ILGLPIGQH+S     + + +EVV+ YTP S D + GYFDL++K YPQG +S H
Sbjct: 87  LPRPTDILGLPIGQHISLAATIEGQPKEVVRSYTPISSDNEAGYFDLLVKAYPQGNISKH 146

Query: 130 FREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILEN-----PVDH 189
              ++ GD + V+GPKG   Y  N     GM+AGGTGITPM QV +AI+ N       D 
Sbjct: 147 LTTLKVGDVMKVRGPKGAMVYTPNMCRHIGMIAGGTGITPMLQVIKAIIRNRPRNGGTDI 206

Query: 190 TNVHLIYANATYEDILLKEELDSLVSRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMIRLH 249
           T V LI+AN   EDILLKEELD L +     F++YYVL+ PP+GW GGVGFVT EMI+  
Sbjct: 207 TKVDLIFANVNPEDILLKEELDKLAAE-DEDFNIYYVLNNPPQGWTGGVGFVTPEMIKER 266

Query: 250 CPGPSPNVRV 254
            P P+ +V+V
Sbjct: 267 LPAPASDVKV 272

[Close]

BLAST of Carg06964 vs. ExPASy Swiss-Prot
Match: Q59P03 (NADH-cytochrome b5 reductase 1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=CBR1 PE=3 SV=1)

HSP 1 Score: 237.7 bits (605), Expect = 1.8e-61
Identity = 114/236 (48.31%), Postives = 152/236 (64.41%), Query Frame = 0


Query: 13  FLTVAVAAVAIGVATAYYFYITKKSKPQGCLDPDNFKEFKLVKRTQLSHNVAKFRFSLPT 72
           F+  A  A  I     YYF + +  K    L PD F++F L+++ ++SHN A +RF LP 
Sbjct: 19  FIVFATVATIISAFIGYYF-LQQSKKHTPVLKPDEFQKFPLIEKIRVSHNSAIYRFGLPK 78

Query: 73  PTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMYPQGRMSHHFREM 132
            T  LGLPIGQH+S  G   +G+EVV+ YTP S D  +G+FDL+IK Y  G +S H    
Sbjct: 79  STDRLGLPIGQHISI-GATIDGKEVVRSYTPISTDDQLGHFDLLIKTYENGNISRHVAGK 138

Query: 133 REGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENPVDHTNVHLIYAN 192
             G+ + ++GPKG F Y  N V++FGM+AGGTGI PM+Q+  AIL+NP D T +HL+YAN
Sbjct: 139 NVGEHIEIRGPKGFFTYTPNMVKSFGMIAGGTGIAPMYQIITAILKNPEDKTKIHLVYAN 198

Query: 193 ATYEDILLKEELDSLVSRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMIRLHCPGPS 249
            T  DILLKEELD+  +R P R  ++YVL++ P  W G VGFVT E+I  H P  S
Sbjct: 199 VTESDILLKEELDNFAARHPDRLKIHYVLNEAPANWQGSVGFVTPEIIDTHLPKAS 252

[Close]

BLAST of Carg06964 vs. ExPASy TrEMBL
Match: A0A6J1FU08 (NADH--cytochrome b5 reductase 1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111448782 PE=4 SV=1)

HSP 1 Score: 617.1 bits (1590), Expect = 4.1e-173
Identity = 297/306 (97.06%), Postives = 299/306 (97.71%), Query Frame = 0


Query: 1   MATSFVHSHRPEFLTVAVAAVAIGVATAYYFYITKKSKPQGCLDPDNFKEFKLVKRTQLS 60
           MATSFVHSHRPEFLTVAVAAVAIGVATAYYFYITKKSKPQGCLDPDNFKEFKLVKR QLS
Sbjct: 1   MATSFVHSHRPEFLTVAVAAVAIGVATAYYFYITKKSKPQGCLDPDNFKEFKLVKRKQLS 60

Query: 61  HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMY 120
           HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMY
Sbjct: 61  HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMY 120

Query: 121 PQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENP 180
           PQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENP
Sbjct: 121 PQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENP 180

Query: 181 VDHTNVHLIYANATYEDILLKEELDSLVSRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMI 240
           VDHTNVHLIYANATYEDILLKEELDSL SRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMI
Sbjct: 181 VDHTNVHLIYANATYEDILLKEELDSLASRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMI 240

Query: 241 RLHCPGPSPNVRVDIEVRTTPHEQSYGCLPQNYWLYFPNAVPVLIQPTPLFTLLFADLIQ 300
           RLHCPGPSPNVRVDIEVRTTPHEQSYGCLPQNYWLYFPNAVPVLIQPTPLFTLLFA L +
Sbjct: 241 RLHCPGPSPNVRVDIEVRTTPHEQSYGCLPQNYWLYFPNAVPVLIQPTPLFTLLFALLAR 300

Query: 301 SSHPHP 307
           S+   P
Sbjct: 301 SNSIFP 306

[Close]

BLAST of Carg06964 vs. ExPASy TrEMBL
Match: A0A6J1JE65 (NADH--cytochrome b5 reductase 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111483718 PE=4 SV=1)

HSP 1 Score: 611.7 bits (1576), Expect = 1.7e-171
Identity = 294/306 (96.08%), Postives = 296/306 (96.73%), Query Frame = 0


Query: 1   MATSFVHSHRPEFLTVAVAAVAIGVATAYYFYITKKSKPQGCLDPDNFKEFKLVKRTQLS 60
           MATSFVHSHRPEFLTVAVAAVAIGVAT YYFYITKKSKPQGCLDPD FKEFKLVKRTQLS
Sbjct: 1   MATSFVHSHRPEFLTVAVAAVAIGVATTYYFYITKKSKPQGCLDPDKFKEFKLVKRTQLS 60

Query: 61  HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMY 120
           HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMY
Sbjct: 61  HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMY 120

Query: 121 PQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENP 180
           PQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENP
Sbjct: 121 PQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENP 180

Query: 181 VDHTNVHLIYANATYEDILLKEELDSLVSRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMI 240
           VDHTN HLIYANATYEDILLKEELDSL SRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMI
Sbjct: 181 VDHTNAHLIYANATYEDILLKEELDSLASRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMI 240

Query: 241 RLHCPGPSPNVRVDIEVRTTPHEQSYGCLPQNYWLYFPNAVPVLIQPTPLFTLLFADLIQ 300
           RLHCPGPSPNVRVDIEVRTTPHEQSYGCLPQNYWLY PNAVPVLIQPTPLFTLLFA L +
Sbjct: 241 RLHCPGPSPNVRVDIEVRTTPHEQSYGCLPQNYWLYLPNAVPVLIQPTPLFTLLFALLAR 300

Query: 301 SSHPHP 307
           S+   P
Sbjct: 301 SNSIFP 306

[Close]

BLAST of Carg06964 vs. ExPASy TrEMBL
Match: A0A6J1FVC6 (NADH--cytochrome b5 reductase 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111448782 PE=4 SV=1)

HSP 1 Score: 562.4 bits (1448), Expect = 1.2e-156
Identity = 276/306 (90.20%), Postives = 278/306 (90.85%), Query Frame = 0


Query: 1   MATSFVHSHRPEFLTVAVAAVAIGVATAYYFYITKKSKPQGCLDPDNFKEFKLVKRTQLS 60
           MATSFVHSHRPEFLTVAVAAVAIGVATAYYFYITKKSKPQGCLDPDNFKEFKLVKR QLS
Sbjct: 1   MATSFVHSHRPEFLTVAVAAVAIGVATAYYFYITKKSKPQGCLDPDNFKEFKLVKRKQLS 60

Query: 61  HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMY 120
           HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMY
Sbjct: 61  HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMY 120

Query: 121 PQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENP 180
           PQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENP
Sbjct: 121 PQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENP 180

Query: 181 VDHTNVHLIYANATYEDILLKEELDSLVSRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMI 240
           VDHTNVHLIYANATYEDILLK                      PPEGWDGGVGFVTEEMI
Sbjct: 181 VDHTNVHLIYANATYEDILLK----------------------PPEGWDGGVGFVTEEMI 240

Query: 241 RLHCPGPSPNVRVDIEVRTTPHEQSYGCLPQNYWLYFPNAVPVLIQPTPLFTLLFADLIQ 300
           RLHCPGPSPNVRVDIEVRTTPHEQSYGCLPQNYWLYFPNAVPVLIQPTPLFTLLFA L +
Sbjct: 241 RLHCPGPSPNVRVDIEVRTTPHEQSYGCLPQNYWLYFPNAVPVLIQPTPLFTLLFALLAR 284

Query: 301 SSHPHP 307
           S+   P
Sbjct: 301 SNSIFP 284

[Close]

BLAST of Carg06964 vs. ExPASy TrEMBL
Match: A0A6J1JC74 (NADH--cytochrome b5 reductase 1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111483718 PE=4 SV=1)

HSP 1 Score: 557.0 bits (1434), Expect = 5.0e-155
Identity = 273/306 (89.22%), Postives = 275/306 (89.87%), Query Frame = 0


Query: 1   MATSFVHSHRPEFLTVAVAAVAIGVATAYYFYITKKSKPQGCLDPDNFKEFKLVKRTQLS 60
           MATSFVHSHRPEFLTVAVAAVAIGVAT YYFYITKKSKPQGCLDPD FKEFKLVKRTQLS
Sbjct: 1   MATSFVHSHRPEFLTVAVAAVAIGVATTYYFYITKKSKPQGCLDPDKFKEFKLVKRTQLS 60

Query: 61  HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMY 120
           HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMY
Sbjct: 61  HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMY 120

Query: 121 PQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENP 180
           PQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENP
Sbjct: 121 PQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENP 180

Query: 181 VDHTNVHLIYANATYEDILLKEELDSLVSRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMI 240
           VDHTN HLIYANATYEDILLK                      PPEGWDGGVGFVTEEMI
Sbjct: 181 VDHTNAHLIYANATYEDILLK----------------------PPEGWDGGVGFVTEEMI 240

Query: 241 RLHCPGPSPNVRVDIEVRTTPHEQSYGCLPQNYWLYFPNAVPVLIQPTPLFTLLFADLIQ 300
           RLHCPGPSPNVRVDIEVRTTPHEQSYGCLPQNYWLY PNAVPVLIQPTPLFTLLFA L +
Sbjct: 241 RLHCPGPSPNVRVDIEVRTTPHEQSYGCLPQNYWLYLPNAVPVLIQPTPLFTLLFALLAR 284

Query: 301 SSHPHP 307
           S+   P
Sbjct: 301 SNSIFP 284

[Close]

BLAST of Carg06964 vs. ExPASy TrEMBL
Match: A0A6J1FXX1 (NADH-cytochrome b5 reductase OS=Cucurbita moschata OX=3662 GN=LOC111448782 PE=3 SV=1)

HSP 1 Score: 521.2 bits (1341), Expect = 3.0e-144
Identity = 250/253 (98.81%), Postives = 251/253 (99.21%), Query Frame = 0


Query: 1   MATSFVHSHRPEFLTVAVAAVAIGVATAYYFYITKKSKPQGCLDPDNFKEFKLVKRTQLS 60
           MATSFVHSHRPEFLTVAVAAVAIGVATAYYFYITKKSKPQGCLDPDNFKEFKLVKR QLS
Sbjct: 1   MATSFVHSHRPEFLTVAVAAVAIGVATAYYFYITKKSKPQGCLDPDNFKEFKLVKRKQLS 60

Query: 61  HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMY 120
           HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMY
Sbjct: 61  HNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKMY 120

Query: 121 PQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENP 180
           PQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENP
Sbjct: 121 PQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILENP 180

Query: 181 VDHTNVHLIYANATYEDILLKEELDSLVSRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMI 240
           VDHTNVHLIYANATYEDILLKEELDSL SRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMI
Sbjct: 181 VDHTNVHLIYANATYEDILLKEELDSLASRFPSRFSVYYVLDQPPEGWDGGVGFVTEEMI 240

Query: 241 RLHCPGPSPNVRV 254
           RLHCPGPSPNVR+
Sbjct: 241 RLHCPGPSPNVRI 253

[Close]

BLAST of Carg06964 vs. TAIR 10
Match: AT5G17770.1 (NADH:cytochrome B5 reductase 1 )

HSP 1 Score: 369.8 bits (948), Expect = 2.2e-102
Identity = 174/254 (68.50%), Postives = 208/254 (81.89%), Query Frame = 0


Query: 1   MATSFVHS-HRPEFLTVAVAAVAIGVATAYYFYITKKSKPQGCLDPDNFKEFKLVKRTQL 60
           M T F+ +  R   L V VA VA+G   AY  ++T   K + CLDP+NFKEFKLVKR QL
Sbjct: 1   MDTEFLRTLDRQILLGVFVAFVAVGAGAAY--FLTSSKKRRVCLDPENFKEFKLVKRHQL 60

Query: 61  SHNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVGYFDLVIKM 120
           SHNVAKF F LPT TS+LGLPIGQH+SCRGKD +GE+V+KPYTPT+LD+DVG F+LVIKM
Sbjct: 61  SHNVAKFVFELPTSTSVLGLPIGQHISCRGKDGQGEDVIKPYTPTTLDSDVGRFELVIKM 120

Query: 121 YPQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARAILEN 180
           YPQGRMSHHFREMR GD LAVKGPKGRFKY+  +  AFGMLAGG+GITPMFQVARAILEN
Sbjct: 121 YPQGRMSHHFREMRVGDHLAVKGPKGRFKYQPGQFRAFGMLAGGSGITPMFQVARAILEN 180

Query: 181 PVDHTNVHLIYANATYEDILLKEELDSLVSRFPSRFSVYYVLDQPPEGWDGGVGFVTEEM 240
           P D T VHLIYAN TY+DILLKEEL+ L + +P +F ++YVL+QPPE WDGGVGFV++EM
Sbjct: 181 PTDKTKVHLIYANVTYDDILLKEELEGLTTNYPEQFKIFYVLNQPPEVWDGGVGFVSKEM 240

Query: 241 IRLHCPGPSPNVRV 254
           I+ HCP P+ ++++
Sbjct: 241 IQTHCPAPASDIQI 252

[Close]

BLAST of Carg06964 vs. TAIR 10
Match: AT5G20080.1 (FAD/NAD(P)-binding oxidoreductase )

HSP 1 Score: 196.4 bits (498), Expect = 3.3e-50
Identity = 105/258 (40.70%), Postives = 160/258 (62.02%), Query Frame = 0


Query: 13  FLTVAVAAVAIGVATAYYFYIT------------KKSKPQGCLDPDNFKEFKLVKRTQLS 72
           F T A+AA++ G +   Y+Y+T            +++ P+  L+PD + EFKL    ++S
Sbjct: 34  FSTGAIAALSGGFS---YYYLTSGNNLVYLDQAKEETGPKTALNPDKWLEFKLQDTARVS 93

Query: 73  HNVAKFRFSLPTPTSILGLPIGQHMSCR---GKDSEGEE--VVKPYTPTSLDTDVGYFDL 132
           HN   FRFS   P++ LGL +   +  R   G ++EG+   V++PYTP S     GYFDL
Sbjct: 94  HNTQLFRFSF-DPSAELGLHVASCLLTRAPLGYNAEGKTKYVIRPYTPISDPEAKGYFDL 153

Query: 133 VIKMYPQGRMSHHFREMREGDFLAVKGPKGRFKYEANEVEAFGMLAGGTGITPMFQVARA 192
           +IK+YP G+MS HF  ++ GD L VKGP  +FKY  N  +  GM+AGG+GITPM QV  A
Sbjct: 154 LIKVYPDGKMSQHFASLKPGDVLEVKGPVEKFKYSPNMKKHIGMIAGGSGITPMLQVIDA 213

Query: 193 ILENPVDHTNVHLIYANATYEDILLKEELDSLVSRFPSRFSVYYVLDQPPEGWDGGVGFV 252
           I++NP D+T + L+YAN + +DILLK++LD L +  P+   ++Y +D P + W GGVG++
Sbjct: 214 IVKNPEDNTQISLLYANVSPDDILLKQKLDVLQANHPN-LKIFYTVDNPTKNWKGGVGYI 273

Query: 253 TEEMIRLHCPGPSPNVRV 254
           +++M     P P+ +  +
Sbjct: 274 SKDMALKGLPLPTDDTLI 286

[Close]

BLAST of Carg06964 vs. TAIR 10
Match: AT1G37130.1 (nitrate reductase 2 )

HSP 1 Score: 168.7 bits (426), Expect = 7.3e-42
Identity = 86/213 (40.38%), Postives = 131/213 (61.50%), Query Frame = 0


Query: 52  KLVKRTQLSHNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVG 111
           +LV++T +SH+V KFRF+LP    +LGLP+G+H+      ++ +  ++ YTP+S    VG
Sbjct: 666 QLVEKTSISHDVRKFRFALPVEDMVLGLPVGKHIFLCATIND-KLCLRAYTPSSTVDVVG 725

Query: 112 YFDLVIKMY-----PQ----GRMSHHFREMREGDFLAVKGPKGRFKY----------EAN 171
           YF+LV+K+Y     P+    G MS +   +  G  L +KGP G  +Y          +  
Sbjct: 726 YFELVVKIYFGGVHPRFPNGGLMSQYLDSLPIGSTLEIKGPLGHVEYLGKGSFTVHGKPK 785

Query: 172 EVEAFGMLAGGTGITPMFQVARAILENPVDHTNVHLIYANATYEDILLKEELDSLVSRFP 231
             +   MLAGGTGITP++Q+ +AIL++P D T +++IYAN T EDILL+EELD    ++P
Sbjct: 786 FADKLAMLAGGTGITPVYQIIQAILKDPEDETEMYVIYANRTEEDILLREELDGWAEQYP 845

Query: 232 SRFSVYYVLDQPPEGWDGGVGFVTEEMIRLHCP 246
            R  V+YV++   EGW    GF++E ++R H P
Sbjct: 846 DRLKVWYVVESAKEGWAYSTGFISEAIMREHIP 877

[Close]

BLAST of Carg06964 vs. TAIR 10
Match: AT1G77760.1 (nitrate reductase 1 )

HSP 1 Score: 162.2 bits (409), Expect = 6.9e-40
Identity = 80/213 (37.56%), Postives = 128/213 (60.09%), Query Frame = 0


Query: 52  KLVKRTQLSHNVAKFRFSLPTPTSILGLPIGQHMSCRGKDSEGEEVVKPYTPTSLDTDVG 111
           +L+++T +SH+V KFRF+LP+    LGLP+G+H+      ++ +  ++ YTPTS    VG
Sbjct: 666 RLIEKTSISHDVRKFRFALPSEDQQLGLPVGKHVFVCANIND-KLCLRAYTPTSAIDAVG 725

Query: 112 YFDLVIKMY-----PQ----GRMSHHFREMREGDFLAVKGPKGRFKYEANE--------- 171
           + DLV+K+Y     P+    G MS H   +  G  + +KGP G  +Y+            
Sbjct: 726 HIDLVVKVYFKDVHPRFPNGGLMSQHLDSLPIGSMIDIKGPLGHIEYKGKGNFLVSGKPK 785

Query: 172 -VEAFGMLAGGTGITPMFQVARAILENPVDHTNVHLIYANATYEDILLKEELDSLVSRFP 231
             +   MLAGGTGITP++Q+ ++IL +P D T ++++YAN T +DIL++EEL+   S+  
Sbjct: 786 FAKKLAMLAGGTGITPIYQIIQSILSDPEDETEMYVVYANRTEDDILVREELEGWASKHK 845

Query: 232 SRFSVYYVLDQPPEGWDGGVGFVTEEMIRLHCP 246
            R  ++YV++   EGW    GF+TE ++R H P
Sbjct: 846 ERLKIWYVVEIAKEGWSYSTGFITEAVLREHIP 877

The following BLAST results are available for this feature:

BLAST of Carg06964 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7010210.1	1.3e-181	100.00	NADH--cytochrome b5 reductase 1 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022944291.1	8.4e-173	97.06	NADH--cytochrome b5 reductase 1-like isoform X1 [Cucurbita moschata]	[more]
XP_022985759.1	3.5e-171	96.08	NADH--cytochrome b5 reductase 1-like isoform X1 [Cucurbita maxima]	[more]
XP_022944292.1	2.5e-156	90.20	NADH--cytochrome b5 reductase 1-like isoform X2 [Cucurbita moschata]	[more]
XP_022985760.1	1.0e-154	89.22	NADH--cytochrome b5 reductase 1-like isoform X2 [Cucurbita maxima]	[more]

BLAST of Carg06964 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZNT1	3.1e-101	68.50	NADH--cytochrome b5 reductase 1 OS=Arabidopsis thaliana OX=3702 GN=CBR1 PE=1 SV=...	[more]
Q6BUX2	1.5e-63	49.79	NADH-cytochrome b5 reductase 1 OS=Debaryomyces hansenii (strain ATCC 36239 / CBS...	[more]
Q6CA86	2.8e-62	48.77	NADH-cytochrome b5 reductase 1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) ...	[more]
A1C7E9	8.1e-62	49.20	NADH-cytochrome b5 reductase 1 OS=Aspergillus clavatus (strain ATCC 1007 / CBS 5...	[more]
Q59P03	1.8e-61	48.31	NADH-cytochrome b5 reductase 1 OS=Candida albicans (strain SC5314 / ATCC MYA-287...	[more]

BLAST of Carg06964 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FU08	4.1e-173	97.06	NADH--cytochrome b5 reductase 1-like isoform X1 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1JE65	1.7e-171	96.08	NADH--cytochrome b5 reductase 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1FVC6	1.2e-156	90.20	NADH--cytochrome b5 reductase 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1JC74	5.0e-155	89.22	NADH--cytochrome b5 reductase 1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1FXX1	3.0e-144	98.81	NADH-cytochrome b5 reductase OS=Cucurbita moschata OX=3662 GN=LOC111448782 PE=3 ...	[more]

BLAST of Carg06964 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G17770.1	2.2e-102	68.50	NADH:cytochrome B5 reductase 1	[more]
AT5G20080.1	3.3e-50	40.70	FAD/NAD(P)-binding oxidoreductase	[more]
AT1G37130.1	7.3e-42	40.38	nitrate reductase 2	[more]
AT1G77760.1	6.9e-40	37.56	nitrate reductase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001834	NADH:cytochrome b5 reductase-like	PRINTS	PR00406	CYTB5RDTASE	coord: 158..177 score: 66.67 coord: 135..149 score: 49.44 coord: 77..88 score: 53.47 coord: 196..207 score: 64.76 coord: 99..106 score: 57.29
IPR008333	Flavoprotein pyridine nucleotide cytochrome reductase-like, FAD-binding domain	PFAM	PF00970	FAD_binding_6	coord: 51..149 e-value: 9.2E-32 score: 109.4
IPR001433	Oxidoreductase FAD/NAD(P)-binding	PFAM	PF00175	NAD_binding_1	coord: 159..246 e-value: 1.2E-23 score: 83.9
None	No IPR available	GENE3D	2.40.30.10	Translation factors	coord: 41..141 e-value: 1.3E-34 score: 120.8
None	No IPR available	PANTHER	PTHR19370:SF204	NADH-CYTOCHROME B5 REDUCTASE	coord: 6..253
None	No IPR available	PANTHER	PTHR19370	NADH-CYTOCHROME B5 REDUCTASE	coord: 6..253
None	No IPR available	CDD	cd06183	cyt_b5_reduct_like	coord: 51..253 e-value: 8.29522E-100 score: 291.008
IPR039261	Ferredoxin-NADP reductase (FNR), nucleotide-binding domain	GENE3D	3.40.50.80		coord: 142..255 e-value: 3.7E-32 score: 113.0
IPR039261	Ferredoxin-NADP reductase (FNR), nucleotide-binding domain	SUPERFAMILY	52343	Ferredoxin reductase-like, C-terminal NADP-linked domain	coord: 138..252
IPR017927	FAD-binding domain, ferredoxin reductase-type	PROSITE	PS51384	FAD_FR	coord: 46..150 score: 14.751878
IPR017938	Riboflavin synthase-like beta-barrel	SUPERFAMILY	63380	Riboflavin synthase domain-like	coord: 42..150

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg06964-RA	Carg06964-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0022900	electron transport chain
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004128	cytochrome-b5 reductase activity, acting on NAD(P)H
molecular_function	GO:0016491	oxidoreductase activity