Carg06861 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg06861
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Tryptophan synthase
Location	Carg_Chr18: 1730432 .. 1732445 (-)
RNA-Seq Expression	Carg06861
Synteny	Carg06861

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GAGGAGCTGGAGGTGGCGCTGAGGGACTTTGTTGGGCGAGAGACGCCGCTGTATCACGCTGAGAGGCTGACAAAGTACTACAAAGACGAGGAAGGAAAAGGCCCCGAGATTTACATAAAAAGAGAGGATCTGAACCATTGCGGTGCACACAAGATGAACAATGCAATAGCGCAGGTTATGATTGCCAAGCGCATGGGGCGGAGGCGTGTGGTTGCCGCCACTGGGGCTGGCCAGCATGGTGTTGCCACCGCAGCTGCCTGCGCCAAACATGATTTAGAGTGCACCATTTTGATGGGCTCTGACGATATCAAGAAGCAATCCTCAAATGTTGTCTTAATCAAGCTACTCGGTGCTCAGGTACTCCATTAATTTGTCAAAAGTATTGTTGGAAGGGAATCCAAATCGACTAATTTAGGAAATGATCATGAATTTATAAACAAAGAATATATGTCCATATGTATGAGGCCTTTTTGGATAGTCCAAAGCCCACGAGAGCTTATGCTCAAAGTGGACAATATCATATATCATCGTGGAGATTCGTGATTCCTAACAGATTTAATATCTCAGGTGAAATCTGTAGAAGGAAACTTCAAGGATGCGTCGTCGGAGGCGATAAGAGAGTGGGTCGGGGACTTGGAAACGAGCTATTACTTGACGGGGACAGTGGTAGGGCCGCACCCATGTCCAGCTATGGTGAGGGAGTTTCAGTCAGTGATAGGAAAAGAGACGAGAAAACAAGCCATGGAGAAATGGGGAGGCTTACCAGACGTGCTTGTGGCATGCATAGGGAGTGGCTCAAACGCGTTGGGACTGTTCAATGAGTTCATCAAAGAAGAAGATGTGCGGTTGATTGGAGTTGAGGCTGCTGGGTTTGGATTGGATAGTGGAAAGCACTCAGCTACTTTGTCTAAAGGACATGTTGGGGTTTACCATGGAGCTTTGAGCTATCTTCTCCAAGATGATGAAGGCCAAATCCTTGTCCCTCATTCCGTCGGCGTAGGGTACGTATGTTAGAAATTACGAACTTCCATAATGTTCTCATATTATCCATTTTGGGTGGAGTTTTCTCAACTGGCATCGTACCAATGCAGATGTATTCCTTATTTATAAACTCATTATTAACCCTTAATTAACCGATGTGGGACTCTTCTCTCAATAATCTTCAACAATTCTCTCGAATAAAGTACACCATAGAGCCTCCCCCGAAGCCTATGGAGCATTCGAACCGTCTATCCTTAATCAAGACTTGACTTCTTCTCTAGAGTCCTTAACAATCCATCCCTCGAATAAAGTACATCATAGAGCCTTCCCTGATATCTATAGAGCCCTTAAACAACCTCTCTCCCTAATCGAGTCTCGATTCTTTCTCTAAAACACTCAAATAAAGTACACCCGCTATTCAATATTTCAGTCACTTTCGACTACACTTTCAAGACTCACAAGTTCTGTATTCAACATTTGAGGATTCTATTGACATGATTAAGTTAATGGCATGAATCTAATACTATATTAGAAATCACGAACCTCCATAATGGTATGATATTGTCCACTTTGAGCATTAACTCTCGTGGCTCTACTTTTAGTTTCTCCAAAAGGTTTCATACCAATGCATATATATTCTTTACTTATAAACTCATGATCAACCCCTTAATTAGTCGACGCGAGACTACTCTCCCATTAATCCTCACCAAACTACTTTAAATTTCGAACTTTTTTGAATTGCAGACTGGAATACCCAGGAGTAGGACCAGAGCTGAGCTTCTTAAAAGAGAGTGGCAGAGGTGAATTTCACACAGCAATAGACAAAGAAGCAGTGGAAGCTTACAAACGGGTATGCAAATTGGAAGGCATTTTTCCATCATTGGAAGCTTCTCATGCCTTTGCTTATCTCGACAAGCTTTGCCCCACCTTGCGCGACGGCTGCAAGGTGGTGGTCAACTGCAGTGGCCGTGGTGACAAAGATGCTGCCATAGTCTTCAATTATCAACACTATCAAGAACACTACATATTTTAA

mRNA sequence

GAGGAGCTGGAGGTGGCGCTGAGGGACTTTGTTGGGCGAGAGACGCCGCTGTATCACGCTGAGAGGCTGACAAAGTACTACAAAGACGAGGAAGGAAAAGGCCCCGAGATTTACATAAAAAGAGAGGATCTGAACCATTGCGGTGCACACAAGATGAACAATGCAATAGCGCAGGTTATGATTGCCAAGCGCATGGGGCGGAGGCGTGTGGTTGCCGCCACTGGGGCTGGCCAGCATGGTGTTGCCACCGCAGCTGCCTGCGCCAAACATGATTTAGAGTGCACCATTTTGATGGGCTCTGACGATATCAAGAAGCAATCCTCAAATGTTGTCTTAATCAAGCTACTCGGTGCTCAGGTGAAATCTGTAGAAGGAAACTTCAAGGATGCGTCGTCGGAGGCGATAAGAGAGTGGGTCGGGGACTTGGAAACGAGCTATTACTTGACGGGGACAGTGGTAGGGCCGCACCCATGTCCAGCTATGGTGAGGGAGTTTCAGTCAGTGATAGGAAAAGAGACGAGAAAACAAGCCATGGAGAAATGGGGAGGCTTACCAGACGTGCTTGTGGCATGCATAGGGAGTGGCTCAAACGCGTTGGGACTGTTCAATGAGTTCATCAAAGAAGAAGATGTGCGGTTGATTGGAGTTGAGGCTGCTGGGTTTGGATTGGATAGTGGAAAGCACTCAGCTACTTTGTCTAAAGGACATGTTGGGGTTTACCATGGAGCTTTGAGCTATCTTCTCCAAGATGATGAAGGCCAAATCCTTGTCCCTCATTCCGTCGGCGTAGGACTGGAATACCCAGGAGTAGGACCAGAGCTGAGCTTCTTAAAAGAGAGTGGCAGAGGTGAATTTCACACAGCAATAGACAAAGAAGCAGTGGAAGCTTACAAACGGGTATGCAAATTGGAAGGCATTTTTCCATCATTGGAAGCTTCTCATGCCTTTGCTTATCTCGACAAGCTTTGCCCCACCTTGCGCGACGGCTGCAAGGTGGTGGTCAACTGCAGTGGCCGTGGTGACAAAGATGCTGCCATAGTCTTCAATTATCAACACTATCAAGAACACTACATATTTTAA

Coding sequence (CDS)

GAGGAGCTGGAGGTGGCGCTGAGGGACTTTGTTGGGCGAGAGACGCCGCTGTATCACGCTGAGAGGCTGACAAAGTACTACAAAGACGAGGAAGGAAAAGGCCCCGAGATTTACATAAAAAGAGAGGATCTGAACCATTGCGGTGCACACAAGATGAACAATGCAATAGCGCAGGTTATGATTGCCAAGCGCATGGGGCGGAGGCGTGTGGTTGCCGCCACTGGGGCTGGCCAGCATGGTGTTGCCACCGCAGCTGCCTGCGCCAAACATGATTTAGAGTGCACCATTTTGATGGGCTCTGACGATATCAAGAAGCAATCCTCAAATGTTGTCTTAATCAAGCTACTCGGTGCTCAGGTGAAATCTGTAGAAGGAAACTTCAAGGATGCGTCGTCGGAGGCGATAAGAGAGTGGGTCGGGGACTTGGAAACGAGCTATTACTTGACGGGGACAGTGGTAGGGCCGCACCCATGTCCAGCTATGGTGAGGGAGTTTCAGTCAGTGATAGGAAAAGAGACGAGAAAACAAGCCATGGAGAAATGGGGAGGCTTACCAGACGTGCTTGTGGCATGCATAGGGAGTGGCTCAAACGCGTTGGGACTGTTCAATGAGTTCATCAAAGAAGAAGATGTGCGGTTGATTGGAGTTGAGGCTGCTGGGTTTGGATTGGATAGTGGAAAGCACTCAGCTACTTTGTCTAAAGGACATGTTGGGGTTTACCATGGAGCTTTGAGCTATCTTCTCCAAGATGATGAAGGCCAAATCCTTGTCCCTCATTCCGTCGGCGTAGGACTGGAATACCCAGGAGTAGGACCAGAGCTGAGCTTCTTAAAAGAGAGTGGCAGAGGTGAATTTCACACAGCAATAGACAAAGAAGCAGTGGAAGCTTACAAACGGGTATGCAAATTGGAAGGCATTTTTCCATCATTGGAAGCTTCTCATGCCTTTGCTTATCTCGACAAGCTTTGCCCCACCTTGCGCGACGGCTGCAAGGTGGTGGTCAACTGCAGTGGCCGTGGTGACAAAGATGCTGCCATAGTCTTCAATTATCAACACTATCAAGAACACTACATATTTTAA

Protein sequence

EELEVALRDFVGRETPLYHAERLTKYYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVMIAKRMGRRRVVAATGAGQHGVATAAACAKHDLECTILMGSDDIKKQSSNVVLIKLLGAQVKSVEGNFKDASSEAIREWVGDLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEKWGGLPDVLVACIGSGSNALGLFNEFIKEEDVRLIGVEAAGFGLDSGKHSATLSKGHVGVYHGALSYLLQDDEGQILVPHSVGVGLEYPGVGPELSFLKESGRGEFHTAIDKEAVEAYKRVCKLEGIFPSLEASHAFAYLDKLCPTLRDGCKVVVNCSGRGDKDAAIVFNYQHYQEHYIF

Homology

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BLAST of Carg06861 vs. NCBI nr
Match: KAG7012379.1 (Tryptophan synthase beta chain 1, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 731.9 bits (1888), Expect = 2.7e-207
Identity = 359/359 (100.00%), Postives = 359/359 (100.00%), Query Frame = 0


Query: 1   EELEVALRDFVGRETPLYHAERLTKYYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVM 60
           EELEVALRDFVGRETPLYHAERLTKYYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVM
Sbjct: 1   EELEVALRDFVGRETPLYHAERLTKYYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVM 60

Query: 61  IAKRMGRRRVVAATGAGQHGVATAAACAKHDLECTILMGSDDIKKQSSNVVLIKLLGAQV 120
           IAKRMGRRRVVAATGAGQHGVATAAACAKHDLECTILMGSDDIKKQSSNVVLIKLLGAQV
Sbjct: 61  IAKRMGRRRVVAATGAGQHGVATAAACAKHDLECTILMGSDDIKKQSSNVVLIKLLGAQV 120

Query: 121 KSVEGNFKDASSEAIREWVGDLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEK 180
           KSVEGNFKDASSEAIREWVGDLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEK
Sbjct: 121 KSVEGNFKDASSEAIREWVGDLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEK 180

Query: 181 WGGLPDVLVACIGSGSNALGLFNEFIKEEDVRLIGVEAAGFGLDSGKHSATLSKGHVGVY 240
           WGGLPDVLVACIGSGSNALGLFNEFIKEEDVRLIGVEAAGFGLDSGKHSATLSKGHVGVY
Sbjct: 181 WGGLPDVLVACIGSGSNALGLFNEFIKEEDVRLIGVEAAGFGLDSGKHSATLSKGHVGVY 240

Query: 241 HGALSYLLQDDEGQILVPHSVGVGLEYPGVGPELSFLKESGRGEFHTAIDKEAVEAYKRV 300
           HGALSYLLQDDEGQILVPHSVGVGLEYPGVGPELSFLKESGRGEFHTAIDKEAVEAYKRV
Sbjct: 241 HGALSYLLQDDEGQILVPHSVGVGLEYPGVGPELSFLKESGRGEFHTAIDKEAVEAYKRV 300

Query: 301 CKLEGIFPSLEASHAFAYLDKLCPTLRDGCKVVVNCSGRGDKDAAIVFNYQHYQEHYIF 360
           CKLEGIFPSLEASHAFAYLDKLCPTLRDGCKVVVNCSGRGDKDAAIVFNYQHYQEHYIF
Sbjct: 301 CKLEGIFPSLEASHAFAYLDKLCPTLRDGCKVVVNCSGRGDKDAAIVFNYQHYQEHYIF 359

[Close]

BLAST of Carg06861 vs. NCBI nr
Match: XP_022954970.1 (tryptophan synthase beta chain 1-like [Cucurbita moschata])

HSP 1 Score: 706.8 bits (1823), Expect = 9.3e-200
Identity = 347/350 (99.14%), Postives = 349/350 (99.71%), Query Frame = 0


Query: 1   EELEVALRDFVGRETPLYHAERLTKYYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVM 60
           EELEVALRDFVGRETPLYHAERLTKYYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVM
Sbjct: 104 EELEVALRDFVGRETPLYHAERLTKYYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVM 163

Query: 61  IAKRMGRRRVVAATGAGQHGVATAAACAKHDLECTILMGSDDIKKQSSNVVLIKLLGAQV 120
           IAKRMGRRRVVAATGAGQHGVATAAACAKHDLECTILMGS+DIKKQSSNVVLIKLLGAQV
Sbjct: 164 IAKRMGRRRVVAATGAGQHGVATAAACAKHDLECTILMGSEDIKKQSSNVVLIKLLGAQV 223

Query: 121 KSVEGNFKDASSEAIREWVGDLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEK 180
           KSVEGNFKDASSEAIREWVGDLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEK
Sbjct: 224 KSVEGNFKDASSEAIREWVGDLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEK 283

Query: 181 WGGLPDVLVACIGSGSNALGLFNEFIKEEDVRLIGVEAAGFGLDSGKHSATLSKGHVGVY 240
           WGGLPDVLVACIGSGSNALGLFNEFIKEEDVRLIGVEAAGFGLDSGKHSATLSKGHVGVY
Sbjct: 284 WGGLPDVLVACIGSGSNALGLFNEFIKEEDVRLIGVEAAGFGLDSGKHSATLSKGHVGVY 343

Query: 241 HGALSYLLQDDEGQILVPHSVGVGLEYPGVGPELSFLKESGRGEFHTAIDKEAVEAYKRV 300
           HGALSYLLQDDEGQILVPHSVGVGLEYPGVGPELSFLKESGR EFHTAIDKEAVEAYKRV
Sbjct: 344 HGALSYLLQDDEGQILVPHSVGVGLEYPGVGPELSFLKESGRAEFHTAIDKEAVEAYKRV 403

Query: 301 CKLEGIFPSLEASHAFAYLDKLCPTLRDGCKVVVNCSGRGDKDAAIVFNY 351
           C+LEGIFPSLEASHAFAYLDKLCPTLRDGCKVVVNCSGRGDKDAAIVFNY
Sbjct: 404 CRLEGIFPSLEASHAFAYLDKLCPTLRDGCKVVVNCSGRGDKDAAIVFNY 453

[Close]

BLAST of Carg06861 vs. NCBI nr
Match: KAG6573205.1 (Tryptophan synthase beta chain 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 706.1 bits (1821), Expect = 1.6e-199
Identity = 345/355 (97.18%), Postives = 352/355 (99.15%), Query Frame = 0


Query: 1   EELEVALRDFVGRETPLYHAERLTKYYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVM 60
           EELEVALRDFVGRETPLYHAERLTKYYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVM
Sbjct: 105 EELEVALRDFVGRETPLYHAERLTKYYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVM 164

Query: 61  IAKRMGRRRVVAATGAGQHGVATAAACAKHDLECTILMGSDDIKKQSSNVVLIKLLGAQV 120
           IAKRMGRRRVVAATGAGQHGVATAAACAKHDLECTILMGS+DIKKQSSNVVLIKLLGAQV
Sbjct: 165 IAKRMGRRRVVAATGAGQHGVATAAACAKHDLECTILMGSEDIKKQSSNVVLIKLLGAQV 224

Query: 121 KSVEGNFKDASSEAIREWVGDLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEK 180
           KSVEGNFKDASSEAIREWVG+LETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEK
Sbjct: 225 KSVEGNFKDASSEAIREWVGNLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEK 284

Query: 181 WGGLPDVLVACIGSGSNALGLFNEFIKEEDVRLIGVEAAGFGLDSGKHSATLSKGHVGVY 240
           WGGLPDVLVACIGSGSNALGLFNEF+KEEDVRLIGVEAAGFGLDSGKHSATLSKGHVGVY
Sbjct: 285 WGGLPDVLVACIGSGSNALGLFNEFMKEEDVRLIGVEAAGFGLDSGKHSATLSKGHVGVY 344

Query: 241 HGALSYLLQDDEGQILVPHSVGVGLEYPGVGPELSFLKESGRGEFHTAIDKEAVEAYKRV 300
           HGALSYLLQD+EGQILVPHSVG+GLEYPGVGPELSFLKESGR EFHTAIDKEAVEAYKRV
Sbjct: 345 HGALSYLLQDEEGQILVPHSVGIGLEYPGVGPELSFLKESGRAEFHTAIDKEAVEAYKRV 404

Query: 301 CKLEGIFPSLEASHAFAYLDKLCPTLRDGCKVVVNCSGRGDKDAAIVFNYQHYQE 356
           C+LEGIFPSLEASHAFAYLDKLCPTL DGCKVVVNCSGRGDKDAAIVFNY H+QE
Sbjct: 405 CRLEGIFPSLEASHAFAYLDKLCPTLTDGCKVVVNCSGRGDKDAAIVFNYGHHQE 459

[Close]

BLAST of Carg06861 vs. NCBI nr
Match: XP_023542665.1 (tryptophan synthase beta chain 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 701.8 bits (1810), Expect = 3.0e-198
Identity = 344/355 (96.90%), Postives = 349/355 (98.31%), Query Frame = 0


Query: 1   EELEVALRDFVGRETPLYHAERLTKYYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVM 60
           EELEVALRDFVGRETPLYHAERLTKYYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVM
Sbjct: 105 EELEVALRDFVGRETPLYHAERLTKYYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVM 164

Query: 61  IAKRMGRRRVVAATGAGQHGVATAAACAKHDLECTILMGSDDIKKQSSNVVLIKLLGAQV 120
           IAKRMGRRRVVAATGAGQHGVATAAACAKHDLECTI MGS+DIKKQSSNVVLIKLLGAQV
Sbjct: 165 IAKRMGRRRVVAATGAGQHGVATAAACAKHDLECTIFMGSEDIKKQSSNVVLIKLLGAQV 224

Query: 121 KSVEGNFKDASSEAIREWVGDLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEK 180
           KSVEGNFKDASSEAIREWVG+LE SYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEK
Sbjct: 225 KSVEGNFKDASSEAIREWVGNLEMSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEK 284

Query: 181 WGGLPDVLVACIGSGSNALGLFNEFIKEEDVRLIGVEAAGFGLDSGKHSATLSKGHVGVY 240
           WGGLP+VLVACIGSGSNALGLFNEF+ EEDVRLIGVEAAGFGLDSGKHSATLSKGHVGVY
Sbjct: 285 WGGLPEVLVACIGSGSNALGLFNEFMNEEDVRLIGVEAAGFGLDSGKHSATLSKGHVGVY 344

Query: 241 HGALSYLLQDDEGQILVPHSVGVGLEYPGVGPELSFLKESGRGEFHTAIDKEAVEAYKRV 300
           HGALSYLLQDDEGQILVPHSVGVGLEYPGVGPELSFLKESGR EFHTAIDKEAVEAYKRV
Sbjct: 345 HGALSYLLQDDEGQILVPHSVGVGLEYPGVGPELSFLKESGRAEFHTAIDKEAVEAYKRV 404

Query: 301 CKLEGIFPSLEASHAFAYLDKLCPTLRDGCKVVVNCSGRGDKDAAIVFNYQHYQE 356
           CKLEGIFPSLEASHAFAYLDKLCPTL DGCKVVVNCSGRGDKDAAIVFNY H+QE
Sbjct: 405 CKLEGIFPSLEASHAFAYLDKLCPTLTDGCKVVVNCSGRGDKDAAIVFNYGHHQE 459

[Close]

BLAST of Carg06861 vs. NCBI nr
Match: XP_022954968.1 (tryptophan synthase beta chain 1-like [Cucurbita moschata])

HSP 1 Score: 696.4 bits (1796), Expect = 1.3e-196
Identity = 341/355 (96.06%), Postives = 349/355 (98.31%), Query Frame = 0


Query: 1   EELEVALRDFVGRETPLYHAERLTKYYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVM 60
           EELEVALRDFVGRETPLY+AERLT++YKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVM
Sbjct: 105 EELEVALRDFVGRETPLYYAERLTRHYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVM 164

Query: 61  IAKRMGRRRVVAATGAGQHGVATAAACAKHDLECTILMGSDDIKKQSSNVVLIKLLGAQV 120
           IAKRMGRRRVVAATGAGQHGVATAAACAKHDLECTI MGS+DIKKQSSNVVLIKLLGAQV
Sbjct: 165 IAKRMGRRRVVAATGAGQHGVATAAACAKHDLECTIFMGSEDIKKQSSNVVLIKLLGAQV 224

Query: 121 KSVEGNFKDASSEAIREWVGDLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEK 180
           KSVEGNFKDASSEAIREWVG+LE SYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEK
Sbjct: 225 KSVEGNFKDASSEAIREWVGNLERSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEK 284

Query: 181 WGGLPDVLVACIGSGSNALGLFNEFIKEEDVRLIGVEAAGFGLDSGKHSATLSKGHVGVY 240
           WGGLPDVLVACIGSGSNALGLFNEFI EEDVRLIGVEAAGFGLDSGKHSATLSKGHVGVY
Sbjct: 285 WGGLPDVLVACIGSGSNALGLFNEFINEEDVRLIGVEAAGFGLDSGKHSATLSKGHVGVY 344

Query: 241 HGALSYLLQDDEGQILVPHSVGVGLEYPGVGPELSFLKESGRGEFHTAIDKEAVEAYKRV 300
           HGALSYLLQDDEGQILVPHSVGVGLEYPGVGPELSFLKESGR EFHTAIDKEAVEAYKRV
Sbjct: 345 HGALSYLLQDDEGQILVPHSVGVGLEYPGVGPELSFLKESGRAEFHTAIDKEAVEAYKRV 404

Query: 301 CKLEGIFPSLEASHAFAYLDKLCPTLRDGCKVVVNCSGRGDKDAAIVFNYQHYQE 356
           C+LEGIFPSLEASHAFAYLD+LCPTL DGCKVVVNCSGRGDKDAAIVFNY H+QE
Sbjct: 405 CRLEGIFPSLEASHAFAYLDELCPTLTDGCKVVVNCSGRGDKDAAIVFNYGHHQE 459

[Close]

BLAST of Carg06861 vs. ExPASy Swiss-Prot
Match: O50046 (Tryptophan synthase beta chain 2, chloroplastic OS=Camptotheca acuminata OX=16922 GN=TSB PE=2 SV=1)

HSP 1 Score: 493.4 bits (1269), Expect = 2.1e-138
Identity = 226/346 (65.32%), Postives = 293/346 (84.68%), Query Frame = 0


Query: 1   EELEVALRDFVGRETPLYHAERLTKYYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVM 60
           +EL+  L+D+VGRE+PLY AERLT +YK   G+GPEIY+KREDLNH GAHK+NNA+AQ +
Sbjct: 111 KELDGILKDYVGRESPLYFAERLTLHYKRPNGEGPEIYLKREDLNHTGAHKINNAVAQAL 170

Query: 61  IAKRMGRRRVVAATGAGQHGVATAAACAKHDLECTILMGSDDIKKQSSNVVLIKLLGAQV 120
           +AKR+G++R++A TGAGQHGVATA  CA+  L+C I MG+ D+++Q+ NV  ++LLGA+V
Sbjct: 171 LAKRLGKKRIIAETGAGQHGVATATVCARFGLQCVIYMGAQDMERQALNVFRMRLLGAEV 230

Query: 121 KSVE---GNFKDASSEAIREWVGDLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQA 180
           ++V       KDA+SEAIR+WV ++E+++Y+ G+V GPHP P MVREF +VIGKETRKQA
Sbjct: 231 RAVHSGTATLKDATSEAIRDWVTNVESTHYILGSVAGPHPYPMMVREFHAVIGKETRKQA 290

Query: 181 MEKWGGLPDVLVACIGSGSNALGLFNEFIKEEDVRLIGVEAAGFGLDSGKHSATLSKGHV 240
           +EKWGG PDVLVAC+G GSNA+GLF+EF+ ++DVR+IGVEAAGFGLDSGKH+ATL+KG V
Sbjct: 291 LEKWGGKPDVLVACVGGGSNAMGLFHEFVDDKDVRMIGVEAAGFGLDSGKHAATLTKGEV 350

Query: 241 GVYHGALSYLLQDDEGQILVPHSVGVGLEYPGVGPELSFLKESGRGEFHTAIDKEAVEAY 300
           GV HGA+SYLLQDD+GQI+ PHS+  GL+YPGVGPE SFLK+ GR E++   D+EA+EA+
Sbjct: 351 GVLHGAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSFLKDIGRAEYYCCTDEEALEAF 410

Query: 301 KRVCKLEGIFPSLEASHAFAYLDKLCPTLRDGCKVVVNCSGRGDKD 344
           KR+ +LEGI P+LE SHA A+L+KLCPTL +G KVV+NCSGRGDKD
Sbjct: 411 KRLSRLEGIIPALETSHALAFLEKLCPTLPNGTKVVLNCSGRGDKD 456

[Close]

BLAST of Carg06861 vs. ExPASy Swiss-Prot
Match: P14671 (Tryptophan synthase beta chain 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TSB1 PE=2 SV=1)

HSP 1 Score: 491.5 bits (1264), Expect = 8.1e-138
Identity = 227/352 (64.49%), Postives = 291/352 (82.67%), Query Frame = 0


Query: 2   ELEVALRDFVGRETPLYHAERLTKYYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVMI 61
           EL   L+D+VGRE+PLY AERLT++Y+ E G+GP IY+KREDLNH GAHK+NNA+AQ ++
Sbjct: 116 ELAGILKDYVGRESPLYFAERLTEHYRRENGEGPLIYLKREDLNHTGAHKINNAVAQALL 175

Query: 62  AKRMGRRRVVAATGAGQHGVATAAACAKHDLECTILMGSDDIKKQSSNVVLIKLLGAQVK 121
           AKR+G++R++A TGAGQHGVATA  CA+  LEC I MG+ D+++Q+ NV  ++LLGA+V+
Sbjct: 176 AKRLGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAEVR 235

Query: 122 SVE---GNFKDASSEAIREWVGDLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAM 181
            V       KDA+SEAIR+WV ++ET++Y+ G+V GPHP P MVR+F +VIGKETRKQA+
Sbjct: 236 GVHSGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQAL 295

Query: 182 EKWGGLPDVLVACIGSGSNALGLFNEFIKEEDVRLIGVEAAGFGLDSGKHSATLSKGHVG 241
           EKWGG PDVLVAC+G GSNA+GLF+EF+ + +VR+IGVEAAGFGLDSGKH+ATL+KG VG
Sbjct: 296 EKWGGKPDVLVACVGGGSNAMGLFHEFVNDTEVRMIGVEAAGFGLDSGKHAATLTKGDVG 355

Query: 242 VYHGALSYLLQDDEGQILVPHSVGVGLEYPGVGPELSFLKESGRGEFHTAIDKEAVEAYK 301
           V HGA+SYLLQDD+GQI+ PHS+  GL+YPGVGPE SF K+ GR E+++  D+EA+EA+K
Sbjct: 356 VLHGAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSFFKDMGRAEYYSITDEEALEAFK 415

Query: 302 RVCKLEGIFPSLEASHAFAYLDKLCPTLRDGCKVVVNCSGRGDKDAAIVFNY 351
           RV +LEGI P+LE SHA AYL+KLCPTL DG +VV+N SGRGDKD   V  Y
Sbjct: 416 RVSRLEGIIPALETSHALAYLEKLCPTLSDGTRVVLNFSGRGDKDVQTVAKY 467

[Close]

BLAST of Carg06861 vs. ExPASy Swiss-Prot
Match: P25269 (Tryptophan synthase beta chain 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TSB2 PE=1 SV=2)

HSP 1 Score: 489.2 bits (1258), Expect = 4.0e-137
Identity = 226/352 (64.20%), Postives = 290/352 (82.39%), Query Frame = 0


Query: 2   ELEVALRDFVGRETPLYHAERLTKYYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVMI 61
           EL   L+D+VGRE+PLY AERLT++Y+ E G+GP IY+KREDLNH GAHK+NNA+AQ ++
Sbjct: 121 ELAEILKDYVGRESPLYFAERLTEHYRRENGEGPLIYLKREDLNHTGAHKINNAVAQALL 180

Query: 62  AKRMGRRRVVAATGAGQHGVATAAACAKHDLECTILMGSDDIKKQSSNVVLIKLLGAQVK 121
           AKR+G++R++A TGAGQHGVATA  CA+  L+C I MG+ D+++Q+ NV  ++LLGA+V+
Sbjct: 181 AKRLGKKRIIAETGAGQHGVATATVCARFGLQCIIYMGAQDMERQALNVFRMRLLGAEVR 240

Query: 122 SVE---GNFKDASSEAIREWVGDLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAM 181
            V       KDA+SEAIR+WV ++ET++Y+ G+V GPHP P MVR+F +VIGKETRKQAM
Sbjct: 241 GVHSGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQAM 300

Query: 182 EKWGGLPDVLVACIGSGSNALGLFNEFIKEEDVRLIGVEAAGFGLDSGKHSATLSKGHVG 241
           EKWGG PDVLVAC+G GSNA+GLF+EF+ + +VR+IGVEAAGFGLDSGKH+ATL+KG VG
Sbjct: 301 EKWGGKPDVLVACVGGGSNAMGLFHEFVDDTEVRMIGVEAAGFGLDSGKHAATLTKGDVG 360

Query: 242 VYHGALSYLLQDDEGQILVPHSVGVGLEYPGVGPELSFLKESGRGEFHTAIDKEAVEAYK 301
           V HGA+SYLLQDD+GQI+ PHS+  GL+YPGVGPE SFLK+ GR E+ +  D+EA+EA+K
Sbjct: 361 VLHGAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSFLKDVGRAEYFSVTDEEALEAFK 420

Query: 302 RVCKLEGIFPSLEASHAFAYLDKLCPTLRDGCKVVVNCSGRGDKDAAIVFNY 351
           RV +LEGI P+LE SHA A+L+KLCPTL DG +VV+N SGRGDKD      Y
Sbjct: 421 RVSRLEGIIPALETSHALAHLEKLCPTLPDGARVVLNFSGRGDKDVQTAIKY 472

[Close]

BLAST of Carg06861 vs. ExPASy Swiss-Prot
Match: P43283 (Tryptophan synthase beta chain 1 (Fragment) OS=Zea mays OX=4577 GN=TSB1 PE=2 SV=1)

HSP 1 Score: 486.1 bits (1250), Expect = 3.4e-136
Identity = 223/353 (63.17%), Postives = 291/353 (82.44%), Query Frame = 0


Query: 1   EELEVALRDFVGRETPLYHAERLTKYYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVM 60
           +EL+  L+D+VGRE+PLY AERLT++YK  +G GP IY+KREDLNH GAHK+NNA+AQ +
Sbjct: 34  KELDGILKDYVGRESPLYFAERLTEHYKRADGTGPLIYLKREDLNHRGAHKINNAVAQAL 93

Query: 61  IAKRMGRRRVVAATGAGQHGVATAAACAKHDLECTILMGSDDIKKQSSNVVLIKLLGAQV 120
           +AKR+G++R++A TGAGQHGVATA  CA+  L+C I MG+ D+++Q+ NV  +KLLGA+V
Sbjct: 94  LAKRLGKQRIIAETGAGQHGVATATVCARFGLQCIIYMGAQDMERQALNVFRMKLLGAEV 153

Query: 121 KSVE---GNFKDASSEAIREWVGDLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQA 180
           ++V       KDA+SEAIR+WV ++ET++Y+ G+V GPHP P MVREF  VIGKETR+QA
Sbjct: 154 RAVHSGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVREFHKVIGKETRRQA 213

Query: 181 MEKWGGLPDVLVACIGSGSNALGLFNEFIKEEDVRLIGVEAAGFGLDSGKHSATLSKGHV 240
           M KWGG PDVLVAC+G GSNA+GLF+EF++++DVRLIGVEAAG G+D+ KH+ATL+KG V
Sbjct: 214 MHKWGGKPDVLVACVGGGSNAMGLFHEFVEDQDVRLIGVEAAGHGVDTDKHAATLTKGQV 273

Query: 241 GVYHGALSYLLQDDEGQILVPHSVGVGLEYPGVGPELSFLKESGRGEFHTAIDKEAVEAY 300
           GV HG++SYLLQDD+GQ++ PHS+  GL+YPGVGPE SFLK+ GR E+ +  D+EA++A+
Sbjct: 274 GVLHGSMSYLLQDDDGQVIEPHSISAGLDYPGVGPEHSFLKDIGRAEYDSVTDQEALDAF 333

Query: 301 KRVCKLEGIFPSLEASHAFAYLDKLCPTLRDGCKVVVNCSGRGDKDAAIVFNY 351
           KRV +LEGI P+LE SHA AYL+KLCPTL DG +VV+NCSGRGDKD      Y
Sbjct: 334 KRVSRLEGIIPALETSHALAYLEKLCPTLPDGVRVVLNCSGRGDKDVHTASKY 386

[Close]

BLAST of Carg06861 vs. ExPASy Swiss-Prot
Match: P43284 (Tryptophan synthase beta chain 2, chloroplastic (Fragment) OS=Zea mays OX=4577 GN=TSB2 PE=2 SV=1)

HSP 1 Score: 484.6 bits (1246), Expect = 9.9e-136
Identity = 220/353 (62.32%), Postives = 291/353 (82.44%), Query Frame = 0


Query: 1   EELEVALRDFVGRETPLYHAERLTKYYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVM 60
           +EL+  L+D+VGRE+PLY AERLT++YK  +G GP IY+KREDLNH GAHK+NNA+AQ +
Sbjct: 88  KELDGILKDYVGRESPLYFAERLTEHYKRADGTGPLIYLKREDLNHTGAHKINNAVAQAL 147

Query: 61  IAKRMGRRRVVAATGAGQHGVATAAACAKHDLECTILMGSDDIKKQSSNVVLIKLLGAQV 120
           +AKR+G++R++A TGAGQHGVATA  C +  L+C I MG+ D+++Q+ NV  ++LLGA+V
Sbjct: 148 LAKRLGKQRIIAETGAGQHGVATATVCRRFGLQCIIYMGAQDMERQALNVFRMRLLGAEV 207

Query: 121 KSVE---GNFKDASSEAIREWVGDLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQA 180
           ++V       KDA+SEAIR+WV ++ET++Y+ G+V GPHP P MVREF  VIGKETR+QA
Sbjct: 208 RAVHSGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVREFHKVIGKETRRQA 267

Query: 181 MEKWGGLPDVLVACIGSGSNALGLFNEFIKEEDVRLIGVEAAGFGLDSGKHSATLSKGHV 240
           M+KWGG PDVLVAC+G GSNA+GLF+EF++++DVRL+G+EAAG G+D+ KH+ATL+KG V
Sbjct: 268 MDKWGGKPDVLVACVGGGSNAMGLFHEFVEDQDVRLVGLEAAGHGVDTDKHAATLTKGQV 327

Query: 241 GVYHGALSYLLQDDEGQILVPHSVGVGLEYPGVGPELSFLKESGRGEFHTAIDKEAVEAY 300
           GV HG++SYLLQDD+GQ++ PHS+  GL+YPGVGPE SFLK+ GR E+ +  D+EA++A+
Sbjct: 328 GVLHGSMSYLLQDDDGQVIEPHSISAGLDYPGVGPEHSFLKDIGRAEYDSVTDQEALDAF 387

Query: 301 KRVCKLEGIFPSLEASHAFAYLDKLCPTLRDGCKVVVNCSGRGDKDAAIVFNY 351
           KRV +LEGI P+LE SHA AYL+KLCPTL DG +VVVNCSGRGDKD      Y
Sbjct: 388 KRVSRLEGIIPALETSHALAYLEKLCPTLADGVRVVVNCSGRGDKDVHTASKY 440

[Close]

BLAST of Carg06861 vs. ExPASy TrEMBL
Match: A0A6J1GTV6 (Tryptophan synthase OS=Cucurbita moschata OX=3662 GN=LOC111457069 PE=3 SV=1)

HSP 1 Score: 706.8 bits (1823), Expect = 4.5e-200
Identity = 347/350 (99.14%), Postives = 349/350 (99.71%), Query Frame = 0


Query: 1   EELEVALRDFVGRETPLYHAERLTKYYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVM 60
           EELEVALRDFVGRETPLYHAERLTKYYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVM
Sbjct: 104 EELEVALRDFVGRETPLYHAERLTKYYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVM 163

Query: 61  IAKRMGRRRVVAATGAGQHGVATAAACAKHDLECTILMGSDDIKKQSSNVVLIKLLGAQV 120
           IAKRMGRRRVVAATGAGQHGVATAAACAKHDLECTILMGS+DIKKQSSNVVLIKLLGAQV
Sbjct: 164 IAKRMGRRRVVAATGAGQHGVATAAACAKHDLECTILMGSEDIKKQSSNVVLIKLLGAQV 223

Query: 121 KSVEGNFKDASSEAIREWVGDLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEK 180
           KSVEGNFKDASSEAIREWVGDLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEK
Sbjct: 224 KSVEGNFKDASSEAIREWVGDLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEK 283

Query: 181 WGGLPDVLVACIGSGSNALGLFNEFIKEEDVRLIGVEAAGFGLDSGKHSATLSKGHVGVY 240
           WGGLPDVLVACIGSGSNALGLFNEFIKEEDVRLIGVEAAGFGLDSGKHSATLSKGHVGVY
Sbjct: 284 WGGLPDVLVACIGSGSNALGLFNEFIKEEDVRLIGVEAAGFGLDSGKHSATLSKGHVGVY 343

Query: 241 HGALSYLLQDDEGQILVPHSVGVGLEYPGVGPELSFLKESGRGEFHTAIDKEAVEAYKRV 300
           HGALSYLLQDDEGQILVPHSVGVGLEYPGVGPELSFLKESGR EFHTAIDKEAVEAYKRV
Sbjct: 344 HGALSYLLQDDEGQILVPHSVGVGLEYPGVGPELSFLKESGRAEFHTAIDKEAVEAYKRV 403

Query: 301 CKLEGIFPSLEASHAFAYLDKLCPTLRDGCKVVVNCSGRGDKDAAIVFNY 351
           C+LEGIFPSLEASHAFAYLDKLCPTLRDGCKVVVNCSGRGDKDAAIVFNY
Sbjct: 404 CRLEGIFPSLEASHAFAYLDKLCPTLRDGCKVVVNCSGRGDKDAAIVFNY 453

[Close]

BLAST of Carg06861 vs. ExPASy TrEMBL
Match: A0A6J1GSL9 (Tryptophan synthase OS=Cucurbita moschata OX=3662 GN=LOC111457067 PE=3 SV=1)

HSP 1 Score: 696.4 bits (1796), Expect = 6.1e-197
Identity = 341/355 (96.06%), Postives = 349/355 (98.31%), Query Frame = 0


Query: 1   EELEVALRDFVGRETPLYHAERLTKYYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVM 60
           EELEVALRDFVGRETPLY+AERLT++YKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVM
Sbjct: 105 EELEVALRDFVGRETPLYYAERLTRHYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVM 164

Query: 61  IAKRMGRRRVVAATGAGQHGVATAAACAKHDLECTILMGSDDIKKQSSNVVLIKLLGAQV 120
           IAKRMGRRRVVAATGAGQHGVATAAACAKHDLECTI MGS+DIKKQSSNVVLIKLLGAQV
Sbjct: 165 IAKRMGRRRVVAATGAGQHGVATAAACAKHDLECTIFMGSEDIKKQSSNVVLIKLLGAQV 224

Query: 121 KSVEGNFKDASSEAIREWVGDLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEK 180
           KSVEGNFKDASSEAIREWVG+LE SYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEK
Sbjct: 225 KSVEGNFKDASSEAIREWVGNLERSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEK 284

Query: 181 WGGLPDVLVACIGSGSNALGLFNEFIKEEDVRLIGVEAAGFGLDSGKHSATLSKGHVGVY 240
           WGGLPDVLVACIGSGSNALGLFNEFI EEDVRLIGVEAAGFGLDSGKHSATLSKGHVGVY
Sbjct: 285 WGGLPDVLVACIGSGSNALGLFNEFINEEDVRLIGVEAAGFGLDSGKHSATLSKGHVGVY 344

Query: 241 HGALSYLLQDDEGQILVPHSVGVGLEYPGVGPELSFLKESGRGEFHTAIDKEAVEAYKRV 300
           HGALSYLLQDDEGQILVPHSVGVGLEYPGVGPELSFLKESGR EFHTAIDKEAVEAYKRV
Sbjct: 345 HGALSYLLQDDEGQILVPHSVGVGLEYPGVGPELSFLKESGRAEFHTAIDKEAVEAYKRV 404

Query: 301 CKLEGIFPSLEASHAFAYLDKLCPTLRDGCKVVVNCSGRGDKDAAIVFNYQHYQE 356
           C+LEGIFPSLEASHAFAYLD+LCPTL DGCKVVVNCSGRGDKDAAIVFNY H+QE
Sbjct: 405 CRLEGIFPSLEASHAFAYLDELCPTLTDGCKVVVNCSGRGDKDAAIVFNYGHHQE 459

[Close]

BLAST of Carg06861 vs. ExPASy TrEMBL
Match: A0A6J1K0T4 (Tryptophan synthase OS=Cucurbita maxima OX=3661 GN=LOC111490065 PE=3 SV=1)

HSP 1 Score: 693.3 bits (1788), Expect = 5.2e-196
Identity = 340/350 (97.14%), Postives = 345/350 (98.57%), Query Frame = 0


Query: 1   EELEVALRDFVGRETPLYHAERLTKYYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVM 60
           EELEVALRDFVGRETPLYHAERLT++YKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVM
Sbjct: 104 EELEVALRDFVGRETPLYHAERLTRHYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVM 163

Query: 61  IAKRMGRRRVVAATGAGQHGVATAAACAKHDLECTILMGSDDIKKQSSNVVLIKLLGAQV 120
           IAKRMGRRRVVAATGAGQHGVATAAACAKHDLECTI MGS+DIKKQSSNVVLI LLGAQV
Sbjct: 164 IAKRMGRRRVVAATGAGQHGVATAAACAKHDLECTIFMGSEDIKKQSSNVVLINLLGAQV 223

Query: 121 KSVEGNFKDASSEAIREWVGDLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEK 180
           KSVEGNFKDASSEAIREWVG+LE SYYL GTVVGPHPCPAMVREFQSVIGKETRKQAMEK
Sbjct: 224 KSVEGNFKDASSEAIREWVGNLEMSYYLMGTVVGPHPCPAMVREFQSVIGKETRKQAMEK 283

Query: 181 WGGLPDVLVACIGSGSNALGLFNEFIKEEDVRLIGVEAAGFGLDSGKHSATLSKGHVGVY 240
           WGGLPDVLVACIGSGSNALGLFNEFIKEEDVRLIGVEAAGFGLDSGKHSATLSKGHVGVY
Sbjct: 284 WGGLPDVLVACIGSGSNALGLFNEFIKEEDVRLIGVEAAGFGLDSGKHSATLSKGHVGVY 343

Query: 241 HGALSYLLQDDEGQILVPHSVGVGLEYPGVGPELSFLKESGRGEFHTAIDKEAVEAYKRV 300
           HGALSYLLQD+EGQILVPHSVGVGLEYPGVGPELSFLKESGR EFHTAIDKEAVEAYKRV
Sbjct: 344 HGALSYLLQDEEGQILVPHSVGVGLEYPGVGPELSFLKESGRAEFHTAIDKEAVEAYKRV 403

Query: 301 CKLEGIFPSLEASHAFAYLDKLCPTLRDGCKVVVNCSGRGDKDAAIVFNY 351
           CKLEGIFPSLEASHAFAYLDKLCPTLRDGCKVVVNCSGRGDKDAAIVFNY
Sbjct: 404 CKLEGIFPSLEASHAFAYLDKLCPTLRDGCKVVVNCSGRGDKDAAIVFNY 453

[Close]

BLAST of Carg06861 vs. ExPASy TrEMBL
Match: A0A5D3BMJ6 (Tryptophan synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G005960 PE=3 SV=1)

HSP 1 Score: 654.1 bits (1686), Expect = 3.5e-184
Identity = 323/356 (90.73%), Postives = 338/356 (94.94%), Query Frame = 0


Query: 1   EELEVALRDFVGRETPLYHAERLTKYYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVM 60
           EELEVALRDFVGRETPLY+AERLTK+YK+EEGKGPEIYIKREDLNHCGAHKMNNAIAQVM
Sbjct: 102 EELEVALRDFVGRETPLYYAERLTKHYKNEEGKGPEIYIKREDLNHCGAHKMNNAIAQVM 161

Query: 61  IAKRMGRRRVVAATGAGQHGVATAAACAKHDLECTILMGSDDIKKQSSNVVLIKLLGAQV 120
           IAKRMGR+ VVAATGAGQHGVATAAACAKHDL+CTI MGS+DI KQSSNV+LIKLLGA+V
Sbjct: 162 IAKRMGRKSVVAATGAGQHGVATAAACAKHDLDCTIFMGSEDINKQSSNVLLIKLLGAKV 221

Query: 121 KSVEGNFKDASSEAIREWVGDLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEK 180
           KSVEGNFKDASSEAIREWVG+LETSYYLTGTVVGP+PCPAMVREFQSVIGKETR+QA EK
Sbjct: 222 KSVEGNFKDASSEAIREWVGNLETSYYLTGTVVGPYPCPAMVREFQSVIGKETRRQAKEK 281

Query: 181 WGGLPDVLVACIGSGSNALGLFNEFIKEEDVRLIGVEAAGFGLDSGKHSATLSKGHVGVY 240
           WG  PDVL+ACIGSGSNALGLF+EFI E+DVRLIGVEAAGFGLDSGKHSATLSKGHVGVY
Sbjct: 282 WGAKPDVLLACIGSGSNALGLFHEFINEKDVRLIGVEAAGFGLDSGKHSATLSKGHVGVY 341

Query: 241 HGALSYLLQDDEGQILVPHSVGVGLEYPGVGPELSFLKESGRGEFHTAIDKEAVEAYKRV 300
           HGALSYLLQDDEGQIL PHSVGVGLEYPGVGPELSFLKESGR EF TA D EAVEAYKR+
Sbjct: 342 HGALSYLLQDDEGQILNPHSVGVGLEYPGVGPELSFLKESGRAEFETASDTEAVEAYKRL 401

Query: 301 CKLEGIFPSLEASHAFAYLDKLCPTLRDGCKVVVNCSGRGDKDAAIVFNYQHYQEH 357
            KLEGIFPSLEASHAFAYL KLCPTL DGCKVVVNCSGRGDKDAAIVFNY H Q H
Sbjct: 402 AKLEGIFPSLEASHAFAYLHKLCPTLPDGCKVVVNCSGRGDKDAAIVFNY-HQQNH 456

[Close]

BLAST of Carg06861 vs. ExPASy TrEMBL
Match: A0A1S3B3A1 (Tryptophan synthase OS=Cucumis melo OX=3656 GN=LOC103485497 PE=3 SV=1)

HSP 1 Score: 654.1 bits (1686), Expect = 3.5e-184
Identity = 323/356 (90.73%), Postives = 338/356 (94.94%), Query Frame = 0


Query: 1   EELEVALRDFVGRETPLYHAERLTKYYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVM 60
           EELEVALRDFVGRETPLY+AERLTK+YK+EEGKGPEIYIKREDLNHCGAHKMNNAIAQVM
Sbjct: 101 EELEVALRDFVGRETPLYYAERLTKHYKNEEGKGPEIYIKREDLNHCGAHKMNNAIAQVM 160

Query: 61  IAKRMGRRRVVAATGAGQHGVATAAACAKHDLECTILMGSDDIKKQSSNVVLIKLLGAQV 120
           IAKRMGR+ VVAATGAGQHGVATAAACAKHDL+CTI MGS+DI KQSSNV+LIKLLGA+V
Sbjct: 161 IAKRMGRKSVVAATGAGQHGVATAAACAKHDLDCTIFMGSEDINKQSSNVLLIKLLGAKV 220

Query: 121 KSVEGNFKDASSEAIREWVGDLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEK 180
           KSVEGNFKDASSEAIREWVG+LETSYYLTGTVVGP+PCPAMVREFQSVIGKETR+QA EK
Sbjct: 221 KSVEGNFKDASSEAIREWVGNLETSYYLTGTVVGPYPCPAMVREFQSVIGKETRRQAKEK 280

Query: 181 WGGLPDVLVACIGSGSNALGLFNEFIKEEDVRLIGVEAAGFGLDSGKHSATLSKGHVGVY 240
           WG  PDVL+ACIGSGSNALGLF+EFI E+DVRLIGVEAAGFGLDSGKHSATLSKGHVGVY
Sbjct: 281 WGAKPDVLLACIGSGSNALGLFHEFINEKDVRLIGVEAAGFGLDSGKHSATLSKGHVGVY 340

Query: 241 HGALSYLLQDDEGQILVPHSVGVGLEYPGVGPELSFLKESGRGEFHTAIDKEAVEAYKRV 300
           HGALSYLLQDDEGQIL PHSVGVGLEYPGVGPELSFLKESGR EF TA D EAVEAYKR+
Sbjct: 341 HGALSYLLQDDEGQILNPHSVGVGLEYPGVGPELSFLKESGRAEFETASDTEAVEAYKRL 400

Query: 301 CKLEGIFPSLEASHAFAYLDKLCPTLRDGCKVVVNCSGRGDKDAAIVFNYQHYQEH 357
            KLEGIFPSLEASHAFAYL KLCPTL DGCKVVVNCSGRGDKDAAIVFNY H Q H
Sbjct: 401 AKLEGIFPSLEASHAFAYLHKLCPTLPDGCKVVVNCSGRGDKDAAIVFNY-HQQNH 455

[Close]

BLAST of Carg06861 vs. TAIR 10
Match: AT5G28237.1 (Pyridoxal-5'-phosphate-dependent enzyme family protein )

HSP 1 Score: 539.3 bits (1388), Expect = 2.4e-153
Identity = 263/347 (75.79%), Postives = 306/347 (88.18%), Query Frame = 0


Query: 1   EELEVALRDFVGRETPLYHAERLTKYYKD----EEGKGPEIYIKREDLNHCGAHKMNNAI 60
           EEL  ALRD+VGRETPLY AERLT++YK+     EG GPEIY+KREDL+HCG+HK+NNA+
Sbjct: 106 EELTTALRDYVGRETPLYFAERLTEHYKNIVPTIEG-GPEIYLKREDLSHCGSHKINNAL 165

Query: 61  AQVMIAKRMGRRRVVAATGAGQHGVATAAACAKHDLECTILMGSDDIKKQSSNVVLIKLL 120
           AQ MI++R+G  RVVAATGAGQHGVATAAACAK  LECT+ MG+ DI+KQS NV+ +KLL
Sbjct: 166 AQAMISRRLGCSRVVAATGAGQHGVATAAACAKLSLECTVFMGAADIEKQSFNVLSMKLL 225

Query: 121 GAQVKSVEGNFKDASSEAIREWVGDLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQ 180
           GAQV SVEG FKDASSEAIR WV +L T+YYL+GTVVGPHPCP +VREFQSVIGKETR+Q
Sbjct: 226 GAQVISVEGTFKDASSEAIRNWVENLYTTYYLSGTVVGPHPCPIIVREFQSVIGKETRRQ 285

Query: 181 AMEKWGGLPDVLVACIGSGSNALGLFNEFIKEEDVRLIGVEAAGFGLDSGKHSATLSKGH 240
           A + WGG PDVLVAC+GSGSNALGLF+EF+ +EDVRL+GVEAAG GLDSGKHSATL+ G 
Sbjct: 286 AKQLWGGKPDVLVACVGSGSNALGLFHEFVGDEDVRLVGVEAAGLGLDSGKHSATLAFGD 345

Query: 241 VGVYHGALSYLLQDDEGQILVPHSVGVGLEYPGVGPELSFLKESGRGEFHTAIDKEAVEA 300
           VGVYHG++SYLLQDD+GQIL PHSVGVGLEYPGVGPE+SF+KE+GR EF+TA D+EA++A
Sbjct: 346 VGVYHGSMSYLLQDDQGQILKPHSVGVGLEYPGVGPEISFMKETGRAEFYTATDEEAIQA 405

Query: 301 YKRVCKLEGIFPSLEASHAFAYLDKLCPTLRDGCKVVVNCSGRGDKD 344
             R+ +LEGI P+LEASHA A+LDKL PTLRDG KVVVNCSGRGDKD
Sbjct: 406 CMRLSRLEGIIPALEASHALAFLDKLVPTLRDGAKVVVNCSGRGDKD 451

[Close]

BLAST of Carg06861 vs. TAIR 10
Match: AT5G54810.1 (tryptophan synthase beta-subunit 1 )

HSP 1 Score: 491.5 bits (1264), Expect = 5.7e-139
Identity = 227/352 (64.49%), Postives = 291/352 (82.67%), Query Frame = 0


Query: 2   ELEVALRDFVGRETPLYHAERLTKYYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVMI 61
           EL   L+D+VGRE+PLY AERLT++Y+ E G+GP IY+KREDLNH GAHK+NNA+AQ ++
Sbjct: 116 ELAGILKDYVGRESPLYFAERLTEHYRRENGEGPLIYLKREDLNHTGAHKINNAVAQALL 175

Query: 62  AKRMGRRRVVAATGAGQHGVATAAACAKHDLECTILMGSDDIKKQSSNVVLIKLLGAQVK 121
           AKR+G++R++A TGAGQHGVATA  CA+  LEC I MG+ D+++Q+ NV  ++LLGA+V+
Sbjct: 176 AKRLGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAEVR 235

Query: 122 SVE---GNFKDASSEAIREWVGDLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAM 181
            V       KDA+SEAIR+WV ++ET++Y+ G+V GPHP P MVR+F +VIGKETRKQA+
Sbjct: 236 GVHSGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQAL 295

Query: 182 EKWGGLPDVLVACIGSGSNALGLFNEFIKEEDVRLIGVEAAGFGLDSGKHSATLSKGHVG 241
           EKWGG PDVLVAC+G GSNA+GLF+EF+ + +VR+IGVEAAGFGLDSGKH+ATL+KG VG
Sbjct: 296 EKWGGKPDVLVACVGGGSNAMGLFHEFVNDTEVRMIGVEAAGFGLDSGKHAATLTKGDVG 355

Query: 242 VYHGALSYLLQDDEGQILVPHSVGVGLEYPGVGPELSFLKESGRGEFHTAIDKEAVEAYK 301
           V HGA+SYLLQDD+GQI+ PHS+  GL+YPGVGPE SF K+ GR E+++  D+EA+EA+K
Sbjct: 356 VLHGAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSFFKDMGRAEYYSITDEEALEAFK 415

Query: 302 RVCKLEGIFPSLEASHAFAYLDKLCPTLRDGCKVVVNCSGRGDKDAAIVFNY 351
           RV +LEGI P+LE SHA AYL+KLCPTL DG +VV+N SGRGDKD   V  Y
Sbjct: 416 RVSRLEGIIPALETSHALAYLEKLCPTLSDGTRVVLNFSGRGDKDVQTVAKY 467

[Close]

BLAST of Carg06861 vs. TAIR 10
Match: AT4G27070.1 (tryptophan synthase beta-subunit 2 )

HSP 1 Score: 489.2 bits (1258), Expect = 2.9e-138
Identity = 226/352 (64.20%), Postives = 290/352 (82.39%), Query Frame = 0


Query: 2   ELEVALRDFVGRETPLYHAERLTKYYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVMI 61
           EL   L+D+VGRE+PLY AERLT++Y+ E G+GP IY+KREDLNH GAHK+NNA+AQ ++
Sbjct: 121 ELAEILKDYVGRESPLYFAERLTEHYRRENGEGPLIYLKREDLNHTGAHKINNAVAQALL 180

Query: 62  AKRMGRRRVVAATGAGQHGVATAAACAKHDLECTILMGSDDIKKQSSNVVLIKLLGAQVK 121
           AKR+G++R++A TGAGQHGVATA  CA+  L+C I MG+ D+++Q+ NV  ++LLGA+V+
Sbjct: 181 AKRLGKKRIIAETGAGQHGVATATVCARFGLQCIIYMGAQDMERQALNVFRMRLLGAEVR 240

Query: 122 SVE---GNFKDASSEAIREWVGDLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAM 181
            V       KDA+SEAIR+WV ++ET++Y+ G+V GPHP P MVR+F +VIGKETRKQAM
Sbjct: 241 GVHSGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQAM 300

Query: 182 EKWGGLPDVLVACIGSGSNALGLFNEFIKEEDVRLIGVEAAGFGLDSGKHSATLSKGHVG 241
           EKWGG PDVLVAC+G GSNA+GLF+EF+ + +VR+IGVEAAGFGLDSGKH+ATL+KG VG
Sbjct: 301 EKWGGKPDVLVACVGGGSNAMGLFHEFVDDTEVRMIGVEAAGFGLDSGKHAATLTKGDVG 360

Query: 242 VYHGALSYLLQDDEGQILVPHSVGVGLEYPGVGPELSFLKESGRGEFHTAIDKEAVEAYK 301
           V HGA+SYLLQDD+GQI+ PHS+  GL+YPGVGPE SFLK+ GR E+ +  D+EA+EA+K
Sbjct: 361 VLHGAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSFLKDVGRAEYFSVTDEEALEAFK 420

Query: 302 RVCKLEGIFPSLEASHAFAYLDKLCPTLRDGCKVVVNCSGRGDKDAAIVFNY 351
           RV +LEGI P+LE SHA A+L+KLCPTL DG +VV+N SGRGDKD      Y
Sbjct: 421 RVSRLEGIIPALETSHALAHLEKLCPTLPDGARVVLNFSGRGDKDVQTAIKY 472

[Close]

BLAST of Carg06861 vs. TAIR 10
Match: AT5G38530.1 (tryptophan synthase beta type 2 )

HSP 1 Score: 99.0 bits (245), Expect = 8.3e-21
Identity = 95/331 (28.70%), Postives = 145/331 (43.81%), Query Frame = 0


Query: 13  RETPLYHAERLTKYYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVMIAKRMGRRRVVA 72
           R TPL  A+RL K  +        IY K E  +  G+HK N A+ Q     + G + VV 
Sbjct: 132 RPTPLIRAKRLEKLLQTP----ARIYFKYEGGSPAGSHKPNTAVPQAYYNAKEGVKNVVT 191

Query: 73  ATGAGQHGVATAAACAKHDLECTILMGSDDIKKQSSNVVLIKLLGAQV------------ 132
            TGAGQ G + A A +   L+C +   ++    +    ++++  GA+V            
Sbjct: 192 ETGAGQWGSSLAFASSLFGLDCEVWQVANSYHTKPYRRLMMQTWGAKVHPSPSDLTEAGR 251

Query: 133 ------KSVEGNFKDASSEAIREWVGDLETSYYLTGTVVGPHPCPAMVREFQSVIGKETR 192
                  S  G+   A SEA+     + +T Y L G+V+        V   Q++IG+E  
Sbjct: 252 RILESDPSSPGSLGIAISEAVEVAARNEDTKYCL-GSVLN------HVLLHQTIIGEECI 311

Query: 193 KQAMEKWGGLPDVLVACIGSGSNALGLFNEFIKEEDVRLIGVEAAGFGLDSGKHSATLSK 252
           +Q ME +G  PD+++ C G GSN  GL   FI+E   +L G               +L+K
Sbjct: 312 QQ-MENFGETPDLIIGCTGGGSNFAGLSFPFIRE---KLKGKINPVIRAVEPSACPSLTK 371

Query: 253 G----HVGVYHGALSYLLQDDEGQILVPHSVGV-GLEYPGVGPELSFLKESGRGEFHTAI 312
           G      G   G    +     G   +P  +   GL Y G+ P +S + E G   F  AI
Sbjct: 372 GVYAYDFGDTAGLTPLMKMHTLGHDFIPDPIHAGGLRYHGMAPLISHVYEQG---FMEAI 431

Query: 313 DKEAVEAYK---RVCKLEGIFPSLEASHAFA 318
               +E ++   +  + EGI P+ E +HA A
Sbjct: 432 SIPQIECFQGAIQFARTEGIIPAPEPTHAIA 444

The following BLAST results are available for this feature:

BLAST of Carg06861 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7012379.1	2.7e-207	100.00	Tryptophan synthase beta chain 1, chloroplastic, partial [Cucurbita argyrosperma...	[more]
XP_022954970.1	9.3e-200	99.14	tryptophan synthase beta chain 1-like [Cucurbita moschata]	[more]
KAG6573205.1	1.6e-199	97.18	Tryptophan synthase beta chain 1, chloroplastic, partial [Cucurbita argyrosperma...	[more]
XP_023542665.1	3.0e-198	96.90	tryptophan synthase beta chain 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022954968.1	1.3e-196	96.06	tryptophan synthase beta chain 1-like [Cucurbita moschata]	[more]

BLAST of Carg06861 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O50046	2.1e-138	65.32	Tryptophan synthase beta chain 2, chloroplastic OS=Camptotheca acuminata OX=1692...	[more]
P14671	8.1e-138	64.49	Tryptophan synthase beta chain 1, chloroplastic OS=Arabidopsis thaliana OX=3702 ...	[more]
P25269	4.0e-137	64.20	Tryptophan synthase beta chain 2, chloroplastic OS=Arabidopsis thaliana OX=3702 ...	[more]
P43283	3.4e-136	63.17	Tryptophan synthase beta chain 1 (Fragment) OS=Zea mays OX=4577 GN=TSB1 PE=2 SV=...	[more]
P43284	9.9e-136	62.32	Tryptophan synthase beta chain 2, chloroplastic (Fragment) OS=Zea mays OX=4577 G...	[more]

BLAST of Carg06861 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GTV6	4.5e-200	99.14	Tryptophan synthase OS=Cucurbita moschata OX=3662 GN=LOC111457069 PE=3 SV=1	[more]
A0A6J1GSL9	6.1e-197	96.06	Tryptophan synthase OS=Cucurbita moschata OX=3662 GN=LOC111457067 PE=3 SV=1	[more]
A0A6J1K0T4	5.2e-196	97.14	Tryptophan synthase OS=Cucurbita maxima OX=3661 GN=LOC111490065 PE=3 SV=1	[more]
A0A5D3BMJ6	3.5e-184	90.73	Tryptophan synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G...	[more]
A0A1S3B3A1	3.5e-184	90.73	Tryptophan synthase OS=Cucumis melo OX=3656 GN=LOC103485497 PE=3 SV=1	[more]

BLAST of Carg06861 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G28237.1	2.4e-153	75.79	Pyridoxal-5'-phosphate-dependent enzyme family protein	[more]
AT5G54810.1	5.7e-139	64.49	tryptophan synthase beta-subunit 1	[more]
AT4G27070.1	2.9e-138	64.20	tryptophan synthase beta-subunit 2	[more]
AT5G38530.1	8.3e-21	28.70	tryptophan synthase beta type 2	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR023026	Tryptophan synthase beta chain/beta chain-like	PIRSF	PIRSF001413	Trp_syn_beta	coord: 1..357 e-value: 9.3E-127 score: 421.5
IPR023026	Tryptophan synthase beta chain/beta chain-like	HAMAP	MF_00133	Trp_synth_beta	coord: 1..351 score: 38.968872
IPR006654	Tryptophan synthase, beta chain	TIGRFAM	TIGR00263	TIGR00263	coord: 2..349 e-value: 1.4E-141 score: 470.0
IPR006654	Tryptophan synthase, beta chain	CDD	cd06446	Trp-synth_B	coord: 1..347 e-value: 0.0 score: 506.688
IPR036052	Tryptophan synthase beta subunit-like PLP-dependent enzyme	GENE3D	3.40.50.1100		coord: 12..344 e-value: 1.3E-147 score: 493.5
IPR036052	Tryptophan synthase beta subunit-like PLP-dependent enzyme	GENE3D	3.40.50.1100		coord: 2..166 e-value: 1.3E-147 score: 493.5
IPR036052	Tryptophan synthase beta subunit-like PLP-dependent enzyme	SUPERFAMILY	53686	Tryptophan synthase beta subunit-like PLP-dependent enzymes	coord: 4..349
IPR001926	Pyridoxal-phosphate dependent enzyme	PFAM	PF00291	PALP	coord: 12..338 e-value: 4.3E-45 score: 154.4
None	No IPR available	PIRSR	PIRSR006278-2	PIRSR006278-2	coord: 35..218 e-value: 3.1E-8 score: 31.3
None	No IPR available	PANTHER	PTHR48077:SF4	TRYPTOPHAN SYNTHASE	coord: 1..353
None	No IPR available	PANTHER	PTHR48077	TRYPTOPHAN SYNTHASE-RELATED	coord: 1..353
IPR006653	Tryptophan synthase, beta chain, conserved site	PROSITE	PS00168	TRP_SYNTHASE_BETA	coord: 44..58

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg06861-RA	Carg06861-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0000162	tryptophan biosynthetic process
biological_process	GO:0006568	tryptophan metabolic process
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0004834	tryptophan synthase activity