Carg06829 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg06829
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	50S ribosomal protein L7/L12
Location	Carg_Chr18: 1515015 .. 1515650 (+)
RNA-Seq Expression	Carg06829
Synteny	Carg06829

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCACTTAGTTTTAAGAACAAGGCTTCTCCTACCCGGTGTGTTTACTAACATCGTTCGTTCCCCTGTGGCTGCCTTCAACTTAAATGGATTACATAGAAATTACGCTCAACCTGCTCGGATAGAGGAGGAAGAGGAGGAAGTGGAAATTGACCAGAGAAGGCTTCCAGCTGATTACGATCCAGCAAATTTCGATCCAACACAGCATCGCAGTCCTCCAACTGATCGTGTTTTTAGGCTTGTGGATGAAATATCAGGACTCACATTAGTTGAAGTTGCTGAATTATCTTCCATTTTGAGGAAAAAATTAGGAATGACAGACATGCCAGTGGTTGGAGTTATGAAACCAGGAGCTGCCGGATTAGCTGGTATGGGTATGAAGGCATCAGCAGCAGCTGCCAAGGAGGAGAAGAAGCCGGAAAAGACTGTTTATGAATTGAAATTAGAGTCTTACGAAGCAGCTGCAAAGATTAAAATTATAAAGGAGGTAAGAAGTTTCACGGATTTAGGGCTTAAAGAAGCAAAGGATCTAGTGGAGAAAACGCCATCAGTGTTGAAGAAGGGATTGTCAAAGGAAGAGGGTGAACAATTGATTGAAAAGATGAAGGCACTCGGTGCAAAAGTCATCTTGGAATGA

mRNA sequence

ATGCACTTAGTTTTAAGAACAAGGCTTCTCCTACCCGGTGTGTTTACTAACATCGTTCGTTCCCCTGTGGCTGCCTTCAACTTAAATGGATTACATAGAAATTACGCTCAACCTGCTCGGATAGAGGAGGAAGAGGAGGAAGTGGAAATTGACCAGAGAAGGCTTCCAGCTGATTACGATCCAGCAAATTTCGATCCAACACAGCATCGCAGTCCTCCAACTGATCGTGTTTTTAGGCTTGTGGATGAAATATCAGGACTCACATTAGTTGAAGTTGCTGAATTATCTTCCATTTTGAGGAAAAAATTAGGAATGACAGACATGCCAGTGGTTGGAGTTATGAAACCAGGAGCTGCCGGATTAGCTGGTATGGGTATGAAGGCATCAGCAGCAGCTGCCAAGGAGGAGAAGAAGCCGGAAAAGACTGTTTATGAATTGAAATTAGAGTCTTACGAAGCAGCTGCAAAGATTAAAATTATAAAGGAGGTAAGAAGTTTCACGGATTTAGGGCTTAAAGAAGCAAAGGATCTAGTGGAGAAAACGCCATCAGTGTTGAAGAAGGGATTGTCAAAGGAAGAGGGTGAACAATTGATTGAAAAGATGAAGGCACTCGGTGCAAAAGTCATCTTGGAATGA

Coding sequence (CDS)

ATGCACTTAGTTTTAAGAACAAGGCTTCTCCTACCCGGTGTGTTTACTAACATCGTTCGTTCCCCTGTGGCTGCCTTCAACTTAAATGGATTACATAGAAATTACGCTCAACCTGCTCGGATAGAGGAGGAAGAGGAGGAAGTGGAAATTGACCAGAGAAGGCTTCCAGCTGATTACGATCCAGCAAATTTCGATCCAACACAGCATCGCAGTCCTCCAACTGATCGTGTTTTTAGGCTTGTGGATGAAATATCAGGACTCACATTAGTTGAAGTTGCTGAATTATCTTCCATTTTGAGGAAAAAATTAGGAATGACAGACATGCCAGTGGTTGGAGTTATGAAACCAGGAGCTGCCGGATTAGCTGGTATGGGTATGAAGGCATCAGCAGCAGCTGCCAAGGAGGAGAAGAAGCCGGAAAAGACTGTTTATGAATTGAAATTAGAGTCTTACGAAGCAGCTGCAAAGATTAAAATTATAAAGGAGGTAAGAAGTTTCACGGATTTAGGGCTTAAAGAAGCAAAGGATCTAGTGGAGAAAACGCCATCAGTGTTGAAGAAGGGATTGTCAAAGGAAGAGGGTGAACAATTGATTGAAAAGATGAAGGCACTCGGTGCAAAAGTCATCTTGGAATGA

Protein sequence

MHLVLRTRLLLPGVFTNIVRSPVAAFNLNGLHRNYAQPARIEEEEEEVEIDQRRLPADYDPANFDPTQHRSPPTDRVFRLVDEISGLTLVEVAELSSILRKKLGMTDMPVVGVMKPGAAGLAGMGMKASAAAAKEEKKPEKTVYELKLESYEAAAKIKIIKEVRSFTDLGLKEAKDLVEKTPSVLKKGLSKEEGEQLIEKMKALGAKVILE

Homology

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BLAST of Carg06829 vs. NCBI nr
Match: KAG7012347.1 (MNP1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 392.9 bits (1008), Expect = 1.8e-105
Identity = 211/211 (100.00%), Postives = 211/211 (100.00%), Query Frame = 0


Query: 1   MHLVLRTRLLLPGVFTNIVRSPVAAFNLNGLHRNYAQPARIEEEEEEVEIDQRRLPADYD 60
           MHLVLRTRLLLPGVFTNIVRSPVAAFNLNGLHRNYAQPARIEEEEEEVEIDQRRLPADYD
Sbjct: 1   MHLVLRTRLLLPGVFTNIVRSPVAAFNLNGLHRNYAQPARIEEEEEEVEIDQRRLPADYD 60

Query: 61  PANFDPTQHRSPPTDRVFRLVDEISGLTLVEVAELSSILRKKLGMTDMPVVGVMKPGAAG 120
           PANFDPTQHRSPPTDRVFRLVDEISGLTLVEVAELSSILRKKLGMTDMPVVGVMKPGAAG
Sbjct: 61  PANFDPTQHRSPPTDRVFRLVDEISGLTLVEVAELSSILRKKLGMTDMPVVGVMKPGAAG 120

Query: 121 LAGMGMKASAAAAKEEKKPEKTVYELKLESYEAAAKIKIIKEVRSFTDLGLKEAKDLVEK 180
           LAGMGMKASAAAAKEEKKPEKTVYELKLESYEAAAKIKIIKEVRSFTDLGLKEAKDLVEK
Sbjct: 121 LAGMGMKASAAAAKEEKKPEKTVYELKLESYEAAAKIKIIKEVRSFTDLGLKEAKDLVEK 180

Query: 181 TPSVLKKGLSKEEGEQLIEKMKALGAKVILE 212
           TPSVLKKGLSKEEGEQLIEKMKALGAKVILE
Sbjct: 181 TPSVLKKGLSKEEGEQLIEKMKALGAKVILE 211

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BLAST of Carg06829 vs. NCBI nr
Match: XP_022954769.1 (uncharacterized protein LOC111456924 [Cucurbita moschata] >XP_022954770.1 uncharacterized protein LOC111456924 [Cucurbita moschata])

HSP 1 Score: 390.2 bits (1001), Expect = 1.1e-104
Identity = 210/211 (99.53%), Postives = 210/211 (99.53%), Query Frame = 0


Query: 1   MHLVLRTRLLLPGVFTNIVRSPVAAFNLNGLHRNYAQPARIEEEEEEVEIDQRRLPADYD 60
           MHLVLRTR LLPGVFTNIVRSPVAAFNLNGLHRNYAQPARIEEEEEEVEIDQRRLPADYD
Sbjct: 1   MHLVLRTRHLLPGVFTNIVRSPVAAFNLNGLHRNYAQPARIEEEEEEVEIDQRRLPADYD 60

Query: 61  PANFDPTQHRSPPTDRVFRLVDEISGLTLVEVAELSSILRKKLGMTDMPVVGVMKPGAAG 120
           PANFDPTQHRSPPTDRVFRLVDEISGLTLVEVAELSSILRKKLGMTDMPVVGVMKPGAAG
Sbjct: 61  PANFDPTQHRSPPTDRVFRLVDEISGLTLVEVAELSSILRKKLGMTDMPVVGVMKPGAAG 120

Query: 121 LAGMGMKASAAAAKEEKKPEKTVYELKLESYEAAAKIKIIKEVRSFTDLGLKEAKDLVEK 180
           LAGMGMKASAAAAKEEKKPEKTVYELKLESYEAAAKIKIIKEVRSFTDLGLKEAKDLVEK
Sbjct: 121 LAGMGMKASAAAAKEEKKPEKTVYELKLESYEAAAKIKIIKEVRSFTDLGLKEAKDLVEK 180

Query: 181 TPSVLKKGLSKEEGEQLIEKMKALGAKVILE 212
           TPSVLKKGLSKEEGEQLIEKMKALGAKVILE
Sbjct: 181 TPSVLKKGLSKEEGEQLIEKMKALGAKVILE 211

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BLAST of Carg06829 vs. NCBI nr
Match: XP_022994471.1 (uncharacterized protein LOC111490181 [Cucurbita maxima])

HSP 1 Score: 384.4 bits (986), Expect = 6.2e-103
Identity = 208/211 (98.58%), Postives = 208/211 (98.58%), Query Frame = 0


Query: 1   MHLVLRTRLLLPGVFTNIVRSPVAAFNLNGLHRNYAQPARIEEEEEEVEIDQRRLPADYD 60
           MHLVLRTR LLPGVFTNIVRSPVAAFNLNGLHRNYAQPA IEEEEEEVEIDQRRLPADYD
Sbjct: 1   MHLVLRTRHLLPGVFTNIVRSPVAAFNLNGLHRNYAQPAWIEEEEEEVEIDQRRLPADYD 60

Query: 61  PANFDPTQHRSPPTDRVFRLVDEISGLTLVEVAELSSILRKKLGMTDMPVVGVMKPGAAG 120
           PANFDPTQHRSPPTDRVFRLVDEISGLTLVEVAELSSILRKKLGMTDMPVVGVMKPGAAG
Sbjct: 61  PANFDPTQHRSPPTDRVFRLVDEISGLTLVEVAELSSILRKKLGMTDMPVVGVMKPGAAG 120

Query: 121 LAGMGMKASAAAAKEEKKPEKTVYELKLESYEAAAKIKIIKEVRSFTDLGLKEAKDLVEK 180
           LAGMGMKASAAAAKE KKPEKTVYELKLESYEAAAKIKIIKEVRSFTDLGLKEAKDLVEK
Sbjct: 121 LAGMGMKASAAAAKEVKKPEKTVYELKLESYEAAAKIKIIKEVRSFTDLGLKEAKDLVEK 180

Query: 181 TPSVLKKGLSKEEGEQLIEKMKALGAKVILE 212
           TPSVLKKGLSKEEGEQLIEKMKALGAKVILE
Sbjct: 181 TPSVLKKGLSKEEGEQLIEKMKALGAKVILE 211

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BLAST of Carg06829 vs. NCBI nr
Match: XP_023542773.1 (uncharacterized protein LOC111802581 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 384.4 bits (986), Expect = 6.2e-103
Identity = 207/211 (98.10%), Postives = 208/211 (98.58%), Query Frame = 0


Query: 1   MHLVLRTRLLLPGVFTNIVRSPVAAFNLNGLHRNYAQPARIEEEEEEVEIDQRRLPADYD 60
           MHLVLRTR LLPGVFTNIVRSPV AFNLNGL RNYAQPARIEEEEEEVEIDQRRLPADYD
Sbjct: 1   MHLVLRTRHLLPGVFTNIVRSPVVAFNLNGLRRNYAQPARIEEEEEEVEIDQRRLPADYD 60

Query: 61  PANFDPTQHRSPPTDRVFRLVDEISGLTLVEVAELSSILRKKLGMTDMPVVGVMKPGAAG 120
           PANFDPTQHRSPPTDRVFRLVDEISGLTLVEVAELSSILRKKLGMTDMPVVGVMKPGAAG
Sbjct: 61  PANFDPTQHRSPPTDRVFRLVDEISGLTLVEVAELSSILRKKLGMTDMPVVGVMKPGAAG 120

Query: 121 LAGMGMKASAAAAKEEKKPEKTVYELKLESYEAAAKIKIIKEVRSFTDLGLKEAKDLVEK 180
           LAGMGMKASAAA+KEEKKPEKTVYELKLESYEAAAKIKIIKEVRSFTDLGLKEAKDLVEK
Sbjct: 121 LAGMGMKASAAASKEEKKPEKTVYELKLESYEAAAKIKIIKEVRSFTDLGLKEAKDLVEK 180

Query: 181 TPSVLKKGLSKEEGEQLIEKMKALGAKVILE 212
           TPSVLKKGLSKEEGEQLIEKMKALGAKVILE
Sbjct: 181 TPSVLKKGLSKEEGEQLIEKMKALGAKVILE 211

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BLAST of Carg06829 vs. NCBI nr
Match: KAG6573163.1 (hypothetical protein SDJN03_27050, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 384.0 bits (985), Expect = 8.2e-103
Identity = 207/209 (99.04%), Postives = 208/209 (99.52%), Query Frame = 0


Query: 3   LVLRTRLLLPGVFTNIVRSPVAAFNLNGLHRNYAQPARIEEEEEEVEIDQRRLPADYDPA 62
           +VLRTR LLPGVFTNIVRSPVAAFNLNGLHRNYAQPARIEEEEEEVEIDQRRLPADYDPA
Sbjct: 42  MVLRTRHLLPGVFTNIVRSPVAAFNLNGLHRNYAQPARIEEEEEEVEIDQRRLPADYDPA 101

Query: 63  NFDPTQHRSPPTDRVFRLVDEISGLTLVEVAELSSILRKKLGMTDMPVVGVMKPGAAGLA 122
           NFDPTQHRSPPTDRVFRLVDEISGLTLVEVAELSSILRKKLGMTDMPVVGVMKPGAAGLA
Sbjct: 102 NFDPTQHRSPPTDRVFRLVDEISGLTLVEVAELSSILRKKLGMTDMPVVGVMKPGAAGLA 161

Query: 123 GMGMKASAAAAKEEKKPEKTVYELKLESYEAAAKIKIIKEVRSFTDLGLKEAKDLVEKTP 182
           GMGMKASAAAAKEEKKPEKTVYELKLESYEAAAKIKIIKEVRSFTDLGLKEAKDLVEKTP
Sbjct: 162 GMGMKASAAAAKEEKKPEKTVYELKLESYEAAAKIKIIKEVRSFTDLGLKEAKDLVEKTP 221

Query: 183 SVLKKGLSKEEGEQLIEKMKALGAKVILE 212
           SVLKKGLSKEEGEQLIEKMKALGAKVILE
Sbjct: 222 SVLKKGLSKEEGEQLIEKMKALGAKVILE 250

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BLAST of Carg06829 vs. ExPASy Swiss-Prot
Match: Q9USJ9 (54S ribosomal protein L12, mitochondrial OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=mrpl12 PE=3 SV=2)

HSP 1 Score: 93.6 bits (231), Expect = 2.9e-18
Identity = 56/136 (41.18%), Postives = 84/136 (61.76%), Query Frame = 0


Query: 76  RVFRLVDEISGLTLVEVAELSSILRKKLGMTDMPVVGVMKPGAAGLAGMGMKASAAAAKE 135
           ++  +VD+IS L+L+E +EL   L++KL + ++  +  + P  A  A      S   A E
Sbjct: 43  QIGEMVDKISSLSLLETSELVKQLKEKLNIAEIAPMAAVAPAVASAA-----PSEEKAPE 102

Query: 136 EKKPEKTVYELKLESYEAAAKIKIIKEVRSFTDLGLKEAKDLVEKTPSVLKKGLSKEEGE 195
           EKK EKT + LKLES++A +K K+IKEV+S   L L +AK  VE  P VLK+ + KE+ E
Sbjct: 103 EKKEEKTTWNLKLESFDAGSKAKVIKEVKSLLGLSLVDAKKFVESAPKVLKENILKEDAE 162

Query: 196 QLIEKMKALGAKVILE 212
            +  K++ L  KV+LE
Sbjct: 163 AIKSKLEKLSCKVVLE 173

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BLAST of Carg06829 vs. ExPASy Swiss-Prot
Match: P41189 (50S ribosomal protein L7/L12 OS=Liberibacter africanus OX=34020 GN=rplL PE=3 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 1.9e-17
Identity = 57/131 (43.51%), Postives = 82/131 (62.60%), Query Frame = 0


Query: 80  LVDEISGLTLVEVAELSSILRKKLGMTDMPVVGVMKPGAAGLAGMGMKASAAAAKEEKKP 139
           +V+++S LTL++ AELS  L ++ G++    V V+             ASAA        
Sbjct: 7   IVEKLSSLTLLQAAELSKRLEEEWGVSAAAPVAVV-------------ASAAGESAAAVA 66

Query: 140 EKTVYELKLESYEAAAKIKIIKEVRSFTDLGLKEAKDLVEKTPSVLKKGLSKEEGEQLIE 199
           EKT +E+ LE ++A  KI +IKEVR+ T+LGLKEAKD VE  P  LK G+SK+E E+L +
Sbjct: 67  EKTEFEVFLEGFDAKKKISVIKEVRAITELGLKEAKDFVESAPKSLKTGVSKDEAEELKK 124

Query: 200 KMKALGAKVIL 211
           K++A GA +IL
Sbjct: 127 KLEAAGATIIL 124

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BLAST of Carg06829 vs. ExPASy Swiss-Prot
Match: A5ELN8 (50S ribosomal protein L7/L12 OS=Bradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182) OX=288000 GN=rplL PE=3 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 3.5e-16
Identity = 57/133 (42.86%), Postives = 85/133 (63.91%), Query Frame = 0


Query: 79  RLVDEISGLTLVEVAELSSILRKKLGMTDMPVVGVMKPGAAGLAGMGMKASAAAAKEEKK 138
           ++VD++S LT++E AEL+ +L +K G++    V V  PGA G         AAAA  E+K
Sbjct: 6   KIVDDLSALTVLEAAELAKLLEEKWGVSAAAAVAVAAPGAGG---------AAAAPAEEK 65

Query: 139 PEKTVYELKLESYEAAAKIKIIKEVRSFTDLGLKEAKDLVEKTPSVLKKGLSKEEGEQLI 198
            E TV    + +     KI++IKEVR+ T LGLKEAKDLVE  P  +K+G++K+E E++ 
Sbjct: 66  TEFTV----VLASAGDKKIEVIKEVRAITGLGLKEAKDLVEGAPKPVKEGVNKDEAEKIK 125

Query: 199 EKMKALGAKVILE 212
            +++  GAKV L+
Sbjct: 126 GQLEKAGAKVELK 125

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BLAST of Carg06829 vs. ExPASy Swiss-Prot
Match: A4YSI0 (50S ribosomal protein L7/L12 OS=Bradyrhizobium sp. (strain ORS 278) OX=114615 GN=rplL PE=3 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 4.6e-16
Identity = 57/133 (42.86%), Postives = 84/133 (63.16%), Query Frame = 0


Query: 79  RLVDEISGLTLVEVAELSSILRKKLGMTDMPVVGVMKPGAAGLAGMGMKASAAAAKEEKK 138
           ++VD++S LT++E AEL+ +L +K G++    V V  PG  G         AAAA  E+K
Sbjct: 6   KIVDDLSSLTVLEAAELAKLLEEKWGVSAAAAVAVAAPGGGG---------AAAAPAEEK 65

Query: 139 PEKTVYELKLESYEAAAKIKIIKEVRSFTDLGLKEAKDLVEKTPSVLKKGLSKEEGEQLI 198
            E TV    + +     KI++IKEVR+ T LGLKEAKDLVE  P  +K+G++KEE E++ 
Sbjct: 66  TEFTV----VLASAGDKKIEVIKEVRAITGLGLKEAKDLVEGAPKPVKEGVNKEEAEKIK 125

Query: 199 EKMKALGAKVILE 212
            +++  GAKV L+
Sbjct: 126 GQLEKAGAKVELK 125

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BLAST of Carg06829 vs. ExPASy Swiss-Prot
Match: A6X0A8 (50S ribosomal protein L7/L12 OS=Ochrobactrum anthropi (strain ATCC 49188 / DSM 6882 / JCM 21032 / NBRC 15819 / NCTC 12168) OX=439375 GN=rplL PE=3 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 1.0e-15
Identity = 59/133 (44.36%), Postives = 84/133 (63.16%), Query Frame = 0


Query: 79  RLVDEISGLTLVEVAELSSILRKKLGMTDMPVVGVMKPGAAGLAGMGMKASAAAAKEEKK 138
           ++VD++S LT++E AELS +L +K G++    V V   G A        A AAAA+    
Sbjct: 6   KIVDDLSALTVLEAAELSKLLEEKWGVSAAAPVAVAAAGGA--------APAAAAE---- 65

Query: 139 PEKTVYELKLESYEAAAKIKIIKEVRSFTDLGLKEAKDLVEKTPSVLKKGLSKEEGEQLI 198
            EKT +++ L     A KI +IKEVR+ T LGLKEAKDLVE  P  +K+G SK+E E++ 
Sbjct: 66  -EKTEFDVVLAD-GGANKINVIKEVRAITGLGLKEAKDLVEGAPKAVKEGASKDEAEKIK 124

Query: 199 EKMKALGAKVILE 212
            +++A GAKV L+
Sbjct: 126 AQLEAAGAKVELK 124

[Close]

BLAST of Carg06829 vs. ExPASy TrEMBL
Match: A0A6J1GU05 (uncharacterized protein LOC111456924 OS=Cucurbita moschata OX=3662 GN=LOC111456924 PE=3 SV=1)

HSP 1 Score: 390.2 bits (1001), Expect = 5.5e-105
Identity = 210/211 (99.53%), Postives = 210/211 (99.53%), Query Frame = 0


Query: 1   MHLVLRTRLLLPGVFTNIVRSPVAAFNLNGLHRNYAQPARIEEEEEEVEIDQRRLPADYD 60
           MHLVLRTR LLPGVFTNIVRSPVAAFNLNGLHRNYAQPARIEEEEEEVEIDQRRLPADYD
Sbjct: 1   MHLVLRTRHLLPGVFTNIVRSPVAAFNLNGLHRNYAQPARIEEEEEEVEIDQRRLPADYD 60

Query: 61  PANFDPTQHRSPPTDRVFRLVDEISGLTLVEVAELSSILRKKLGMTDMPVVGVMKPGAAG 120
           PANFDPTQHRSPPTDRVFRLVDEISGLTLVEVAELSSILRKKLGMTDMPVVGVMKPGAAG
Sbjct: 61  PANFDPTQHRSPPTDRVFRLVDEISGLTLVEVAELSSILRKKLGMTDMPVVGVMKPGAAG 120

Query: 121 LAGMGMKASAAAAKEEKKPEKTVYELKLESYEAAAKIKIIKEVRSFTDLGLKEAKDLVEK 180
           LAGMGMKASAAAAKEEKKPEKTVYELKLESYEAAAKIKIIKEVRSFTDLGLKEAKDLVEK
Sbjct: 121 LAGMGMKASAAAAKEEKKPEKTVYELKLESYEAAAKIKIIKEVRSFTDLGLKEAKDLVEK 180

Query: 181 TPSVLKKGLSKEEGEQLIEKMKALGAKVILE 212
           TPSVLKKGLSKEEGEQLIEKMKALGAKVILE
Sbjct: 181 TPSVLKKGLSKEEGEQLIEKMKALGAKVILE 211

[Close]

BLAST of Carg06829 vs. ExPASy TrEMBL
Match: A0A6J1JZ85 (uncharacterized protein LOC111490181 OS=Cucurbita maxima OX=3661 GN=LOC111490181 PE=3 SV=1)

HSP 1 Score: 384.4 bits (986), Expect = 3.0e-103
Identity = 208/211 (98.58%), Postives = 208/211 (98.58%), Query Frame = 0


Query: 1   MHLVLRTRLLLPGVFTNIVRSPVAAFNLNGLHRNYAQPARIEEEEEEVEIDQRRLPADYD 60
           MHLVLRTR LLPGVFTNIVRSPVAAFNLNGLHRNYAQPA IEEEEEEVEIDQRRLPADYD
Sbjct: 1   MHLVLRTRHLLPGVFTNIVRSPVAAFNLNGLHRNYAQPAWIEEEEEEVEIDQRRLPADYD 60

Query: 61  PANFDPTQHRSPPTDRVFRLVDEISGLTLVEVAELSSILRKKLGMTDMPVVGVMKPGAAG 120
           PANFDPTQHRSPPTDRVFRLVDEISGLTLVEVAELSSILRKKLGMTDMPVVGVMKPGAAG
Sbjct: 61  PANFDPTQHRSPPTDRVFRLVDEISGLTLVEVAELSSILRKKLGMTDMPVVGVMKPGAAG 120

Query: 121 LAGMGMKASAAAAKEEKKPEKTVYELKLESYEAAAKIKIIKEVRSFTDLGLKEAKDLVEK 180
           LAGMGMKASAAAAKE KKPEKTVYELKLESYEAAAKIKIIKEVRSFTDLGLKEAKDLVEK
Sbjct: 121 LAGMGMKASAAAAKEVKKPEKTVYELKLESYEAAAKIKIIKEVRSFTDLGLKEAKDLVEK 180

Query: 181 TPSVLKKGLSKEEGEQLIEKMKALGAKVILE 212
           TPSVLKKGLSKEEGEQLIEKMKALGAKVILE
Sbjct: 181 TPSVLKKGLSKEEGEQLIEKMKALGAKVILE 211

[Close]

BLAST of Carg06829 vs. ExPASy TrEMBL
Match: A0A6J1C7E3 (uncharacterized protein LOC111009067 OS=Momordica charantia OX=3673 GN=LOC111009067 PE=3 SV=1)

HSP 1 Score: 352.1 bits (902), Expect = 1.7e-93
Identity = 193/214 (90.19%), Postives = 198/214 (92.52%), Query Frame = 0


Query: 1   MHLVLRTRLLLPGVFTNIVRSPVAA---FNLNGLHRNYAQPARIEEEEEEVEIDQRRLPA 60
           MHLVLR R LLP VF N +RSP  A    N NGLHRNYAQPARIEEEE+EVEIDQRRLPA
Sbjct: 1   MHLVLRIRHLLPNVFANTIRSPAVALNPLNSNGLHRNYAQPARIEEEEDEVEIDQRRLPA 60

Query: 61  DYDPANFDPTQHRSPPTDRVFRLVDEISGLTLVEVAELSSILRKKLGMTDMPVVGVMKPG 120
           DYDPANFDPT+HRSPPTDRVFRLVDEISGLTLVEVAELS+ILRKKLGMTDMPVVGVMKPG
Sbjct: 61  DYDPANFDPTEHRSPPTDRVFRLVDEISGLTLVEVAELSTILRKKLGMTDMPVVGVMKPG 120

Query: 121 AAGLAGMGMKASAAAAKEEKKPEKTVYELKLESYEAAAKIKIIKEVRSFTDLGLKEAKDL 180
           AAGL GM MKAS  AAKEEKKPEKTVYELKLESYEA+AKIKIIKEVRSFTDLGLKEAKDL
Sbjct: 121 AAGLGGMAMKAS-TAAKEEKKPEKTVYELKLESYEASAKIKIIKEVRSFTDLGLKEAKDL 180

Query: 181 VEKTPSVLKKGLSKEEGEQLIEKMKALGAKVILE 212
           VEKTPSVLKKGLSKEEGEQLIEKMKALGAKVILE
Sbjct: 181 VEKTPSVLKKGLSKEEGEQLIEKMKALGAKVILE 213

[Close]

BLAST of Carg06829 vs. ExPASy TrEMBL
Match: A0A6J1KHE7 (uncharacterized protein LOC111495277 OS=Cucurbita maxima OX=3661 GN=LOC111495277 PE=3 SV=1)

HSP 1 Score: 329.7 bits (844), Expect = 8.8e-87
Identity = 186/214 (86.92%), Postives = 191/214 (89.25%), Query Frame = 0


Query: 1   MHLVLRTRLLLPGVFTNIVRSPVAA---FNLNGLHRNYAQPARIEEEEEEVEIDQRRLPA 60
           M LVLR   LLP V  N  RSP  A    N+NGLHRN+AQPARI  EEEEVEIDQRRLPA
Sbjct: 1   MRLVLRISHLLPNVLANTFRSPALALNPLNVNGLHRNFAQPARI--EEEEVEIDQRRLPA 60

Query: 61  DYDPANFDPTQHRSPPTDRVFRLVDEISGLTLVEVAELSSILRKKLGMTDMPVVGVMKPG 120
           DYD ANFDPTQHRSPPTDRVFRLVDEISGLTLVEVAELSSILRKKLG+TDMPVVGVMK G
Sbjct: 61  DYDLANFDPTQHRSPPTDRVFRLVDEISGLTLVEVAELSSILRKKLGLTDMPVVGVMKAG 120

Query: 121 AAGLAGMGMKASAAAAKEEKKPEKTVYELKLESYEAAAKIKIIKEVRSFTDLGLKEAKDL 180
           AAGLAGM  KAS+A AKEEKK EKTV+ELKLESYEAAAKIKIIKEVRSFTDLGLKEAKDL
Sbjct: 121 AAGLAGMATKASSATAKEEKKTEKTVFELKLESYEAAAKIKIIKEVRSFTDLGLKEAKDL 180

Query: 181 VEKTPSVLKKGLSKEEGEQLIEKMKALGAKVILE 212
           VEKTPSVLKKGLSKEEGEQLIEKMKALGAKVILE
Sbjct: 181 VEKTPSVLKKGLSKEEGEQLIEKMKALGAKVILE 212

[Close]

BLAST of Carg06829 vs. ExPASy TrEMBL
Match: A0A6J1E822 (uncharacterized protein LOC111431646 OS=Cucurbita moschata OX=3662 GN=LOC111431646 PE=3 SV=1)

HSP 1 Score: 329.3 bits (843), Expect = 1.2e-86
Identity = 185/214 (86.45%), Postives = 192/214 (89.72%), Query Frame = 0


Query: 1   MHLVLRTRLLLPGVFTNIVRSPVAA---FNLNGLHRNYAQPARIEEEEEEVEIDQRRLPA 60
           M LVLR   LLP V  N  RSP  A    N+NGLHR+++QPA I EEEEEVEIDQRRLPA
Sbjct: 1   MRLVLRISHLLPNVLANTFRSPALALNPLNVNGLHRDFSQPAWI-EEEEEVEIDQRRLPA 60

Query: 61  DYDPANFDPTQHRSPPTDRVFRLVDEISGLTLVEVAELSSILRKKLGMTDMPVVGVMKPG 120
           DYDPANFDPTQHRSPPTDRVFRLVDEISGLTLVEVAELSSILRK+LGMTDMPVVGVMK G
Sbjct: 61  DYDPANFDPTQHRSPPTDRVFRLVDEISGLTLVEVAELSSILRKRLGMTDMPVVGVMKAG 120

Query: 121 AAGLAGMGMKASAAAAKEEKKPEKTVYELKLESYEAAAKIKIIKEVRSFTDLGLKEAKDL 180
           AAGLAGM  KAS+A AKEEKK EKTV+ELKLESYEAAAKIKIIKEVRSFTDLGLKEAKDL
Sbjct: 121 AAGLAGMATKASSATAKEEKKTEKTVFELKLESYEAAAKIKIIKEVRSFTDLGLKEAKDL 180

Query: 181 VEKTPSVLKKGLSKEEGEQLIEKMKALGAKVILE 212
           VEKTPSVLKKGLSKEEGEQLIEKMKALGAKVILE
Sbjct: 181 VEKTPSVLKKGLSKEEGEQLIEKMKALGAKVILE 213

[Close]

BLAST of Carg06829 vs. TAIR 10
Match: AT1G70190.1 (Ribosomal protein L7/L12, oligomerisation;Ribosomal protein L7/L12, C-terminal/adaptor protein ClpS-like )

HSP 1 Score: 243.0 bits (619), Expect = 2.1e-64
Identity = 138/214 (64.49%), Postives = 168/214 (78.50%), Query Frame = 0


Query: 1   MHLVLRTRLLLPGVFTNIVRSPVAAFNLNGLHRNYAQPARIEEEEEEVEIDQRRLPADYD 60
           M LVLR R  L     +   S ++  +  G +R+Y QPA+IE+EEEE E DQR+LP DYD
Sbjct: 1   MSLVLRFRQHL-----SQTSSRLSLLSRIGGYRSYTQPAKIEDEEEE-EFDQRKLPTDYD 60

Query: 61  PANFDPTQHRSPPTDRVFRLVDEISGLTLVEVAELSSILRKKLGMTDMPVVGVMKPGA-- 120
           PA FDPT+HRSPPTDRVFRLVDEIS LTL+E++EL SI+ KK GMT++P V VMKPGA  
Sbjct: 61  PATFDPTEHRSPPTDRVFRLVDEISSLTLLEISELGSIIMKKKGMTELPTVAVMKPGAGG 120

Query: 121 -AGLAGMGMKASAAAAKEEKKPEKTVYELKLESYEAAAKIKIIKEVRSFTDLGLKEAKDL 180
             G+ G G   S +   EE K EKTV+E+KLES+EA+AKIKIIKE+RSFTDLGLKEAK L
Sbjct: 121 GVGVGGGGGMVSQSGVSEEAKVEKTVFEIKLESFEASAKIKIIKELRSFTDLGLKEAKAL 180

Query: 181 VEKTPSVLKKGLSKEEGEQLIEKMKALGAKVILE 212
           VEKTP++LK GLSKEEGE+++EK+KALGAKV+LE
Sbjct: 181 VEKTPAILKAGLSKEEGEKILEKLKALGAKVVLE 208

[Close]

BLAST of Carg06829 vs. TAIR 10
Match: AT1G70190.2 (Ribosomal protein L7/L12, oligomerisation;Ribosomal protein L7/L12, C-terminal/adaptor protein ClpS-like )

HSP 1 Score: 243.0 bits (619), Expect = 2.1e-64
Identity = 138/214 (64.49%), Postives = 168/214 (78.50%), Query Frame = 0


Query: 1   MHLVLRTRLLLPGVFTNIVRSPVAAFNLNGLHRNYAQPARIEEEEEEVEIDQRRLPADYD 60
           M LVLR R  L     +   S ++  +  G +R+Y QPA+IE+EEEE E DQR+LP DYD
Sbjct: 1   MSLVLRFRQHL-----SQTSSRLSLLSRIGGYRSYTQPAKIEDEEEE-EFDQRKLPTDYD 60

Query: 61  PANFDPTQHRSPPTDRVFRLVDEISGLTLVEVAELSSILRKKLGMTDMPVVGVMKPGA-- 120
           PA FDPT+HRSPPTDRVFRLVDEIS LTL+E++EL SI+ KK GMT++P V VMKPGA  
Sbjct: 61  PATFDPTEHRSPPTDRVFRLVDEISSLTLLEISELGSIIMKKKGMTELPTVAVMKPGAGG 120

Query: 121 -AGLAGMGMKASAAAAKEEKKPEKTVYELKLESYEAAAKIKIIKEVRSFTDLGLKEAKDL 180
             G+ G G   S +   EE K EKTV+E+KLES+EA+AKIKIIKE+RSFTDLGLKEAK L
Sbjct: 121 GVGVGGGGGMVSQSGVSEEAKVEKTVFEIKLESFEASAKIKIIKELRSFTDLGLKEAKAL 180

Query: 181 VEKTPSVLKKGLSKEEGEQLIEKMKALGAKVILE 212
           VEKTP++LK GLSKEEGE+++EK+KALGAKV+LE
Sbjct: 181 VEKTPAILKAGLSKEEGEKILEKLKALGAKVVLE 208

[Close]

BLAST of Carg06829 vs. TAIR 10
Match: AT4G36420.1 (Ribosomal protein L12 family protein )

HSP 1 Score: 136.7 bits (343), Expect = 2.1e-32
Identity = 77/146 (52.74%), Postives = 111/146 (76.03%), Query Frame = 0


Query: 69  HRSPPT--DRVFRLVDEISGLTLVEVAELSSILRKKLGMTDMPVVGVMKPGAAGLAGMGM 128
           + SP T  + V ++V+E+S LTL+E  +L+ ILR+KL ++++PV+  M PG + L G G 
Sbjct: 35  YSSPATQSENVSKIVNELSNLTLLETMDLTEILRQKLNISELPVMAAMMPGMS-LPGSGA 94

Query: 129 KASAAA-AKEEKKPEKTVYELKLESYEAAAKIKIIKEVRSFTDLGLKEAKDLVEKTPSVL 188
             SA    KE+KK  KT +++ L++Y+A +KIK+IKEVR+ T LGLKEAKDLVEK P++L
Sbjct: 95  SKSAGGEGKEKKKEAKTAFDVMLQAYDAVSKIKVIKEVRTITALGLKEAKDLVEKAPTLL 154

Query: 189 KKGLSKEEGEQLIEKMKALGAKVILE 212
           KKG+SKEE E++IEK+KA+GAKV +E
Sbjct: 155 KKGVSKEEAEKIIEKLKAVGAKVAME 179

[Close]

BLAST of Carg06829 vs. TAIR 10
Match: AT4G37660.1 (Ribosomal protein L12/ ATP-dependent Clp protease adaptor protein ClpS family protein )

HSP 1 Score: 107.1 bits (266), Expect = 1.8e-23
Identity = 63/138 (45.65%), Postives = 95/138 (68.84%), Query Frame = 0


Query: 74  TDRVFRLVDEISGLTLVEVAELSSILRKKLGMTDMPVVGVMKPGAAGLAGMGMKASAAAA 133
           T ++ R+ D++  L  +E+ + S +   K+G+         + G+A +A  G    A+A+
Sbjct: 39  TKKLERIADDLLKLNRIELYDYSLLFSHKIGLN--------RYGSA-VAVTGSVGDASAS 98

Query: 134 KEEKKPEKTVYELKLESYEAAAKIKIIKEVRSFTDLGLKEAKDLVEKTPSVLKKGLSKEE 193
            E K  EKT +++KLE +EAA+KIK+IKE+R+FTDLGLKEAK LVEK P ++K GL+KEE
Sbjct: 99  TETKTAEKTTFDVKLEKFEAASKIKVIKEIRAFTDLGLKEAKALVEKAPVIVKNGLTKEE 158

Query: 194 GEQLIEKMKALGAKVILE 212
            E+++EK+KA+GA V LE
Sbjct: 159 AEKIMEKLKAVGAIVALE 167

[Close]

BLAST of Carg06829 vs. TAIR 10
Match: AT3G06040.1 (Ribosomal protein L12/ ATP-dependent Clp protease adaptor protein ClpS family protein )

HSP 1 Score: 104.8 bits (260), Expect = 8.9e-23
Identity = 58/151 (38.41%), Postives = 93/151 (61.59%), Query Frame = 0


Query: 61  PANFDPTQHRSPPTDRVFRLVDEISGLTLVEVAELSSILRKKLGMTDMPVVGVMKPGAAG 120
           P+ +DP   R  P+ ++  L + I+ L+  E  ++   L + L +    ++         
Sbjct: 44  PSVYDPYGPRPQPSSKIMELAERIAALSPEERKQIGPALNEHLRLPKQQMI--------S 103

Query: 121 LAGMGMKASAAAAKEEKKPEKTVYELKLESYEAAAKIKIIKEVRSFTDLGLKEAKDLVEK 180
             G+G K    A   E+K EKT +++KLE + A+ KIK+IKEVR+FT LGLKEAK+LVEK
Sbjct: 104 SDGIGAKQDTGAGNVEEKKEKTAFDVKLEKFNASDKIKVIKEVRTFTSLGLKEAKELVEK 163

Query: 181 TPSVLKKGLSKEEGEQLIEKMKALGAKVILE 212
            P++LK+G++KEE  ++I K+KA+G   ++E
Sbjct: 164 VPAILKQGVTKEEANEIIAKIKAVGGIAVME 186

The following BLAST results are available for this feature:

BLAST of Carg06829 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7012347.1	1.8e-105	100.00	MNP1, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022954769.1	1.1e-104	99.53	uncharacterized protein LOC111456924 [Cucurbita moschata] >XP_022954770.1 unchar...	[more]
XP_022994471.1	6.2e-103	98.58	uncharacterized protein LOC111490181 [Cucurbita maxima]	[more]
XP_023542773.1	6.2e-103	98.10	uncharacterized protein LOC111802581 [Cucurbita pepo subsp. pepo]	[more]
KAG6573163.1	8.2e-103	99.04	hypothetical protein SDJN03_27050, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of Carg06829 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9USJ9	2.9e-18	41.18	54S ribosomal protein L12, mitochondrial OS=Schizosaccharomyces pombe (strain 97...	[more]
P41189	1.9e-17	43.51	50S ribosomal protein L7/L12 OS=Liberibacter africanus OX=34020 GN=rplL PE=3 SV=...	[more]
A5ELN8	3.5e-16	42.86	50S ribosomal protein L7/L12 OS=Bradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182...	[more]
A4YSI0	4.6e-16	42.86	50S ribosomal protein L7/L12 OS=Bradyrhizobium sp. (strain ORS 278) OX=114615 GN...	[more]
A6X0A8	1.0e-15	44.36	50S ribosomal protein L7/L12 OS=Ochrobactrum anthropi (strain ATCC 49188 / DSM 6...	[more]

BLAST of Carg06829 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GU05	5.5e-105	99.53	uncharacterized protein LOC111456924 OS=Cucurbita moschata OX=3662 GN=LOC1114569...	[more]
A0A6J1JZ85	3.0e-103	98.58	uncharacterized protein LOC111490181 OS=Cucurbita maxima OX=3661 GN=LOC111490181...	[more]
A0A6J1C7E3	1.7e-93	90.19	uncharacterized protein LOC111009067 OS=Momordica charantia OX=3673 GN=LOC111009...	[more]
A0A6J1KHE7	8.8e-87	86.92	uncharacterized protein LOC111495277 OS=Cucurbita maxima OX=3661 GN=LOC111495277...	[more]
A0A6J1E822	1.2e-86	86.45	uncharacterized protein LOC111431646 OS=Cucurbita moschata OX=3662 GN=LOC1114316...	[more]

BLAST of Carg06829 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G70190.1	2.1e-64	64.49	Ribosomal protein L7/L12, oligomerisation;Ribosomal protein L7/L12, C-terminal/a...	[more]
AT1G70190.2	2.1e-64	64.49	Ribosomal protein L7/L12, oligomerisation;Ribosomal protein L7/L12, C-terminal/a...	[more]
AT4G36420.1	2.1e-32	52.74	Ribosomal protein L12 family protein	[more]
AT4G37660.1	1.8e-23	45.65	Ribosomal protein L12/ ATP-dependent Clp protease adaptor protein ClpS family pr...	[more]
AT3G06040.1	8.9e-23	38.41	Ribosomal protein L12/ ATP-dependent Clp protease adaptor protein ClpS family pr...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008932	Ribosomal protein L7/L12, oligomerisation	PFAM	PF16320	Ribosomal_L12_N	coord: 80..120 e-value: 1.6E-7 score: 30.9
IPR013823	Ribosomal protein L7/L12, C-terminal	PFAM	PF00542	Ribosomal_L12	coord: 145..210 e-value: 2.6E-20 score: 72.5
IPR036235	Ribosomal protein L7/L12, oligomerisation domain superfamily	GENE3D	1.20.5.710	Single helix bin	coord: 78..139 e-value: 2.1E-6 score: 29.7
IPR036235	Ribosomal protein L7/L12, oligomerisation domain superfamily	SUPERFAMILY	48300	Ribosomal protein L7/12, oligomerisation (N-terminal) domain	coord: 79..120
IPR014719	Ribosomal protein L7/L12, C-terminal/adaptor protein ClpS-like	GENE3D	3.30.1390.10		coord: 140..210 e-value: 1.1E-20 score: 75.5
IPR014719	Ribosomal protein L7/L12, C-terminal/adaptor protein ClpS-like	SUPERFAMILY	54736	ClpS-like	coord: 144..210
IPR000206	Ribosomal protein L7/L12	PANTHER	PTHR45987	39S RIBOSOMAL PROTEIN L12	coord: 23..211
IPR000206	Ribosomal protein L7/L12	HAMAP	MF_00368	Ribosomal_L7_L12	coord: 74..211 score: 17.422144
IPR000206	Ribosomal protein L7/L12	CDD	cd00387	Ribosomal_L7_L12	coord: 76..210 e-value: 1.12714E-42 score: 138.057
IPR015608	Putative ribosomal protein L7/L12, plants	PANTHER	PTHR45987:SF1	F20P5.9 PROTEIN	coord: 23..211

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg06829-RA	Carg06829-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006412	translation
cellular_component	GO:0005840	ribosome
molecular_function	GO:0003735	structural constituent of ribosome