Carg06791 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg06791
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	cycloeucalenol cycloisomerase
Location	Carg_Chr18: 1268421 .. 1269350 (+)
RNA-Seq Expression	Carg06791
Synteny	Carg06791

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AGCAGTCATCCTCAGTCATCGGCTTTGTGCTGTAATCTTGGAATTGCGAAGCCAGAAACTACACTCTTCTCTCTGTTTTCAGATTTCAGTCGGAGAATTTCAAGCCGAGATCGATCAACCTCGTTCAGATTCTTAATCGCTGTAATGGCCGGTGAGATTTCTTCTCTTCGTTTCGTTTTCTTCTGCTTTCGAATCCTGTATGATACTGTTCTTAACAATGTCCAGATCGAGCGCGCGCTCCCGCTCCCGCTCCCTCTCTCGCTCGTTCTTTTAGCCTATGGCTCTCTACGAATCCAAGCAAGAGATGGGGAGAATTGTTTTTTCTTTCCTACACTCCATTTTGGCTAACGCTTTGCTTGGGAATCATCGTCCCTTTCAAACTCTACGAGGTTTGCCCTCTCGAACTCGCTGAAATGGCGCCATTATCAACACTCTTTCTTTTGTTTGATATCACTGTATTGCTTGAGTTTCTGTTCATGTTCGGCTTTGTTTTCGAGAAGTCATCAAGGAAGAATGGAAGTGGCTAGGTTTCAAGATAGAATAGGGATTTAACCAAGAATTGGGACTCGACTGTGTTATGATTAAGCCTTTTCTGTTCTTGAAACCGATAATCATCTAGAAATTGCTTTGAGATGAACTTTTCTCAAATCTTTCTATTATCAAAAGTCTAAACATGATGATTCTGATTGACAGAGTTTCACGGAAATGGAATATATGGTTCTTGGTTTGGTTGCAGCAGTTCCTTCTTTCTTGATACCAGTGGTGTGGCCTGGAGAGGTAGAATTTACTATCTCTTCATTTCTATGATCCGATAACCAAAACCCCTTCACATATTTGTTTATGGGAACCAATGGATTACAGGTTAACTTCATGGGACAGGAAGATTTTCTTCAGTCTAAGGACTGGTGTTTCTCTAAGATTCACATGTGA

mRNA sequence

AGCAGTCATCCTCAGTCATCGGCTTTGTGCTGTAATCTTGGAATTGCGAAGCCAGAAACTACACTCTTCTCTCTGTTTTCAGATTTCAGTCGGAGAATTTCAAGCCGAGATCGATCAACCTCGTTCAGATTCTTAATCGCTGTAATGGCCGATCGAGCGCGCGCTCCCGCTCCCGCTCCCTCTCTCGCTCGTTCTTTTAGCCTATGGCTCTCTACGAATCCAAGCAAGAGATGGGGAGAATTGTTTTTTCTTTCCTACACTCCATTTTGGCTAACGCTTTGCTTGGGAATCATCGTCCCTTTCAAACTCTACGAGAGTTTCACGGAAATGGAATATATGGTTCTTGGTTTGGTTGCAGCAGTTCCTTCTTTCTTGATACCAGTGGTGTGGCCTGGAGAGGTTAACTTCATGGGACAGGAAGATTTTCTTCAGTCTAAGGACTGGTGTTTCTCTAAGATTCACATGTGA

Coding sequence (CDS)

AGCAGTCATCCTCAGTCATCGGCTTTGTGCTGTAATCTTGGAATTGCGAAGCCAGAAACTACACTCTTCTCTCTGTTTTCAGATTTCAGTCGGAGAATTTCAAGCCGAGATCGATCAACCTCGTTCAGATTCTTAATCGCTGTAATGGCCGATCGAGCGCGCGCTCCCGCTCCCGCTCCCTCTCTCGCTCGTTCTTTTAGCCTATGGCTCTCTACGAATCCAAGCAAGAGATGGGGAGAATTGTTTTTTCTTTCCTACACTCCATTTTGGCTAACGCTTTGCTTGGGAATCATCGTCCCTTTCAAACTCTACGAGAGTTTCACGGAAATGGAATATATGGTTCTTGGTTTGGTTGCAGCAGTTCCTTCTTTCTTGATACCAGTGGTGTGGCCTGGAGAGGTTAACTTCATGGGACAGGAAGATTTTCTTCAGTCTAAGGACTGGTGTTTCTCTAAGATTCACATGTGA

Protein sequence

SSHPQSSALCCNLGIAKPETTLFSLFSDFSRRISSRDRSTSFRFLIAVMADRARAPAPAPSLARSFSLWLSTNPSKRWGELFFLSYTPFWLTLCLGIIVPFKLYESFTEMEYMVLGLVAAVPSFLIPVVWPGEVNFMGQEDFLQSKDWCFSKIHM

Homology

[Close]

BLAST of Carg06791 vs. NCBI nr
Match: KAG7012310.1 (Cycloeucalenol cycloisomerase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 316.6 bits (810), Expect = 1.2e-82
Identity = 155/155 (100.00%), Postives = 155/155 (100.00%), Query Frame = 0


Query: 1   SSHPQSSALCCNLGIAKPETTLFSLFSDFSRRISSRDRSTSFRFLIAVMADRARAPAPAP 60
           SSHPQSSALCCNLGIAKPETTLFSLFSDFSRRISSRDRSTSFRFLIAVMADRARAPAPAP
Sbjct: 1   SSHPQSSALCCNLGIAKPETTLFSLFSDFSRRISSRDRSTSFRFLIAVMADRARAPAPAP 60

Query: 61  SLARSFSLWLSTNPSKRWGELFFLSYTPFWLTLCLGIIVPFKLYESFTEMEYMVLGLVAA 120
           SLARSFSLWLSTNPSKRWGELFFLSYTPFWLTLCLGIIVPFKLYESFTEMEYMVLGLVAA
Sbjct: 61  SLARSFSLWLSTNPSKRWGELFFLSYTPFWLTLCLGIIVPFKLYESFTEMEYMVLGLVAA 120

Query: 121 VPSFLIPVVWPGEVNFMGQEDFLQSKDWCFSKIHM 156
           VPSFLIPVVWPGEVNFMGQEDFLQSKDWCFSKIHM
Sbjct: 121 VPSFLIPVVWPGEVNFMGQEDFLQSKDWCFSKIHM 155

[Close]

BLAST of Carg06791 vs. NCBI nr
Match: XP_022954478.1 (cycloeucalenol cycloisomerase [Cucurbita moschata])

HSP 1 Score: 176.8 bits (447), Expect = 1.5e-40
Identity = 85/87 (97.70%), Postives = 86/87 (98.85%), Query Frame = 0


Query: 49  MADRARAPAPAPSLARSFSLWLSTNPSKRWGELFFLSYTPFWLTLCLGIIVPFKLYESFT 108
           MADRARAPAPAPSLARSFSLWLSTNPSKRWGELFFLSYTPFWLTLCLGIIVPFKLYESFT
Sbjct: 1   MADRARAPAPAPSLARSFSLWLSTNPSKRWGELFFLSYTPFWLTLCLGIIVPFKLYESFT 60

Query: 109 EMEYMVLGLVAAVPSFLIPVVWPGEVN 136
           EMEYMVLGLVAAVPSFLIPVVWPGE +
Sbjct: 61  EMEYMVLGLVAAVPSFLIPVVWPGEAD 87

[Close]

BLAST of Carg06791 vs. NCBI nr
Match: KAG6573123.1 (Cycloeucalenol cycloisomerase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 174.1 bits (440), Expect = 9.4e-40
Identity = 84/87 (96.55%), Postives = 85/87 (97.70%), Query Frame = 0


Query: 49  MADRARAPAPAPSLARSFSLWLSTNPSKRWGELFFLSYTPFWLTLCLGIIVPFKLYESFT 108
           MADRARAPAPAPS ARSFSLWLSTNPSKRWGELFFLSYTPFWLTLCLGIIVPFKLYESFT
Sbjct: 1   MADRARAPAPAPSPARSFSLWLSTNPSKRWGELFFLSYTPFWLTLCLGIIVPFKLYESFT 60

Query: 109 EMEYMVLGLVAAVPSFLIPVVWPGEVN 136
           EMEYMVLGLVAAVPSFLIPVVWPGE +
Sbjct: 61  EMEYMVLGLVAAVPSFLIPVVWPGEAD 87

[Close]

BLAST of Carg06791 vs. NCBI nr
Match: XP_023542731.1 (cycloeucalenol cycloisomerase-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 161.0 bits (406), Expect = 8.2e-36
Identity = 80/87 (91.95%), Postives = 81/87 (93.10%), Query Frame = 0


Query: 49  MADRARAPAPAPSLARSFSLWLSTNPSKRWGELFFLSYTPFWLTLCLGIIVPFKLYESFT 108
           MADRARAPA     ARSFSLWLSTNPSKRWGELFFLSYTPFWLTLCLGIIVPFKLYESFT
Sbjct: 1   MADRARAPAS----ARSFSLWLSTNPSKRWGELFFLSYTPFWLTLCLGIIVPFKLYESFT 60

Query: 109 EMEYMVLGLVAAVPSFLIPVVWPGEVN 136
           EMEYMVLGLVAAVPSFLIPVVWPGE +
Sbjct: 61  EMEYMVLGLVAAVPSFLIPVVWPGEAD 83

[Close]

BLAST of Carg06791 vs. NCBI nr
Match: XP_022994443.1 (cycloeucalenol cycloisomerase-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 154.8 bits (390), Expect = 5.9e-34
Identity = 77/87 (88.51%), Postives = 79/87 (90.80%), Query Frame = 0


Query: 49  MADRARAPAPAPSLARSFSLWLSTNPSKRWGELFFLSYTPFWLTLCLGIIVPFKLYESFT 108
           MADRARA       ARSFSLWLS+NPSKRWGELFFLSYTPFWLTLCLGIIVPFKLYESFT
Sbjct: 1   MADRARA------TARSFSLWLSSNPSKRWGELFFLSYTPFWLTLCLGIIVPFKLYESFT 60

Query: 109 EMEYMVLGLVAAVPSFLIPVVWPGEVN 136
           EMEYMVLGLVAAVPSFLIPVVWPGE +
Sbjct: 61  EMEYMVLGLVAAVPSFLIPVVWPGEAD 81

[Close]

BLAST of Carg06791 vs. ExPASy Swiss-Prot
Match: Q9M643 (Cycloeucalenol cycloisomerase OS=Arabidopsis thaliana OX=3702 GN=CPI1 PE=2 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 2.0e-24
Identity = 49/69 (71.01%), Postives = 63/69 (91.30%), Query Frame = 0


Query: 67  SLWLSTNPSKRWGELFFLSYTPFWLTLCLGIIVPFKLYESFTEMEYMVLGLVAAVPSFLI 126
           SLWL+ NPSKRWGELFFL YTPFWLTLCLGI+VP+KLYE+FTE+EY++L LV+AVP+F+I
Sbjct: 8   SLWLAPNPSKRWGELFFLFYTPFWLTLCLGIVVPYKLYETFTELEYLLLALVSAVPAFVI 67

Query: 127 PVVWPGEVN 136
           P++  G+ +
Sbjct: 68  PMLLVGKAD 76

[Close]

BLAST of Carg06791 vs. ExPASy TrEMBL
Match: A0A6J1GR71 (cycloeucalenol cycloisomerase OS=Cucurbita moschata OX=3662 GN=LOC111456735 PE=4 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 7.0e-41
Identity = 85/87 (97.70%), Postives = 86/87 (98.85%), Query Frame = 0


Query: 49  MADRARAPAPAPSLARSFSLWLSTNPSKRWGELFFLSYTPFWLTLCLGIIVPFKLYESFT 108
           MADRARAPAPAPSLARSFSLWLSTNPSKRWGELFFLSYTPFWLTLCLGIIVPFKLYESFT
Sbjct: 1   MADRARAPAPAPSLARSFSLWLSTNPSKRWGELFFLSYTPFWLTLCLGIIVPFKLYESFT 60

Query: 109 EMEYMVLGLVAAVPSFLIPVVWPGEVN 136
           EMEYMVLGLVAAVPSFLIPVVWPGE +
Sbjct: 61  EMEYMVLGLVAAVPSFLIPVVWPGEAD 87

[Close]

BLAST of Carg06791 vs. ExPASy TrEMBL
Match: A0A6J1K570 (cycloeucalenol cycloisomerase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111490163 PE=4 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 2.9e-34
Identity = 77/87 (88.51%), Postives = 79/87 (90.80%), Query Frame = 0


Query: 49  MADRARAPAPAPSLARSFSLWLSTNPSKRWGELFFLSYTPFWLTLCLGIIVPFKLYESFT 108
           MADRARA       ARSFSLWLS+NPSKRWGELFFLSYTPFWLTLCLGIIVPFKLYESFT
Sbjct: 1   MADRARA------TARSFSLWLSSNPSKRWGELFFLSYTPFWLTLCLGIIVPFKLYESFT 60

Query: 109 EMEYMVLGLVAAVPSFLIPVVWPGEVN 136
           EMEYMVLGLVAAVPSFLIPVVWPGE +
Sbjct: 61  EMEYMVLGLVAAVPSFLIPVVWPGEAD 81

[Close]

BLAST of Carg06791 vs. ExPASy TrEMBL
Match: A0A6J1E6Z0 (cycloeucalenol cycloisomerase-like OS=Cucurbita moschata OX=3662 GN=LOC111431284 PE=4 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 9.5e-30
Identity = 78/136 (57.35%), Postives = 96/136 (70.59%), Query Frame = 0


Query: 1   SSHPQSSALCC-NLGIAKPETTLFSLFSDFSRRISSRDRSTSFRFLIAVMADRARAPAPA 60
           S H Q S LCC +    K  +  FSL     +  + + RS S     ++MA   R+    
Sbjct: 15  SRHSQVSDLCCQSWNFRKRFSPRFSLI----QLENFKPRSISLDLNHSLMAAGRRS---- 74

Query: 61  PSLARSFSLWLSTNPSKRWGELFFLSYTPFWLTLCLGIIVPFKLYESFTEMEYMVLGLVA 120
            +  +S SLWLS+NPSKRWGE+FFLSYTPFWLTLCLGI+VPFKLYESFTE+EY++LGLVA
Sbjct: 75  -ARNQSSSLWLSSNPSKRWGEVFFLSYTPFWLTLCLGIVVPFKLYESFTELEYLLLGLVA 134

Query: 121 AVPSFLIPVVWPGEVN 136
           A+PSFLIPVVWPGE +
Sbjct: 135 ALPSFLIPVVWPGEAD 141

[Close]

BLAST of Carg06791 vs. ExPASy TrEMBL
Match: A0A6J1KJU7 (cycloeucalenol cycloisomerase-like OS=Cucurbita maxima OX=3661 GN=LOC111495263 PE=4 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 6.2e-29
Identity = 61/72 (84.72%), Postives = 70/72 (97.22%), Query Frame = 0


Query: 64  RSFSLWLSTNPSKRWGELFFLSYTPFWLTLCLGIIVPFKLYESFTEMEYMVLGLVAAVPS 123
           +S SLWLS+NPSKRWGE+FFLSYTPFWLTLCLGI+VPFKLYESFTE+EY++LGLVAA+PS
Sbjct: 11  QSSSLWLSSNPSKRWGEVFFLSYTPFWLTLCLGIVVPFKLYESFTELEYLLLGLVAALPS 70

Query: 124 FLIPVVWPGEVN 136
           FLIPVVWPGE +
Sbjct: 71  FLIPVVWPGEAD 82

[Close]

BLAST of Carg06791 vs. ExPASy TrEMBL
Match: A0A5A7UM17 (Cycloeucalenol cycloisomerase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold80G001690 PE=4 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 8.9e-28
Identity = 58/71 (81.69%), Postives = 68/71 (95.77%), Query Frame = 0


Query: 65  SFSLWLSTNPSKRWGELFFLSYTPFWLTLCLGIIVPFKLYESFTEMEYMVLGLVAAVPSF 124
           S SLWLS+NPSKRWGELFFLSYTPFWLTLCLGI++PFKLYE FTE+EY++LGL+AA+P+F
Sbjct: 14  SSSLWLSSNPSKRWGELFFLSYTPFWLTLCLGIVIPFKLYERFTELEYLLLGLLAALPAF 73

Query: 125 LIPVVWPGEVN 136
           LIPVVWPGE +
Sbjct: 74  LIPVVWPGEAD 84

[Close]

BLAST of Carg06791 vs. TAIR 10
Match: AT5G50375.1 (cyclopropyl isomerase )

HSP 1 Score: 113.6 bits (283), Expect = 1.4e-25
Identity = 49/69 (71.01%), Postives = 63/69 (91.30%), Query Frame = 0


Query: 67  SLWLSTNPSKRWGELFFLSYTPFWLTLCLGIIVPFKLYESFTEMEYMVLGLVAAVPSFLI 126
           SLWL+ NPSKRWGELFFL YTPFWLTLCLGI+VP+KLYE+FTE+EY++L LV+AVP+F+I
Sbjct: 8   SLWLAPNPSKRWGELFFLFYTPFWLTLCLGIVVPYKLYETFTELEYLLLALVSAVPAFVI 67

Query: 127 PVVWPGEVN 136
           P++  G+ +
Sbjct: 68  PMLLVGKAD 76

[Close]

BLAST of Carg06791 vs. TAIR 10
Match: AT5G50375.2 (cyclopropyl isomerase )

HSP 1 Score: 113.6 bits (283), Expect = 1.4e-25
Identity = 49/69 (71.01%), Postives = 63/69 (91.30%), Query Frame = 0


Query: 67  SLWLSTNPSKRWGELFFLSYTPFWLTLCLGIIVPFKLYESFTEMEYMVLGLVAAVPSFLI 126
           SLWL+ NPSKRWGELFFL YTPFWLTLCLGI+VP+KLYE+FTE+EY++L LV+AVP+F+I
Sbjct: 8   SLWLAPNPSKRWGELFFLFYTPFWLTLCLGIVVPYKLYETFTELEYLLLALVSAVPAFVI 67

Query: 127 PVVWPGEVN 136
           P++  G+ +
Sbjct: 68  PMLLVGKAD 76

The following BLAST results are available for this feature:

BLAST of Carg06791 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7012310.1	1.2e-82	100.00	Cycloeucalenol cycloisomerase, partial [Cucurbita argyrosperma subsp. argyrosper...	[more]
XP_022954478.1	1.5e-40	97.70	cycloeucalenol cycloisomerase [Cucurbita moschata]	[more]
KAG6573123.1	9.4e-40	96.55	Cycloeucalenol cycloisomerase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023542731.1	8.2e-36	91.95	cycloeucalenol cycloisomerase-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022994443.1	5.9e-34	88.51	cycloeucalenol cycloisomerase-like isoform X1 [Cucurbita maxima]	[more]

BLAST of Carg06791 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M643	2.0e-24	71.01	Cycloeucalenol cycloisomerase OS=Arabidopsis thaliana OX=3702 GN=CPI1 PE=2 SV=1	[more]

BLAST of Carg06791 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GR71	7.0e-41	97.70	cycloeucalenol cycloisomerase OS=Cucurbita moschata OX=3662 GN=LOC111456735 PE=4...	[more]
A0A6J1K570	2.9e-34	88.51	cycloeucalenol cycloisomerase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC...	[more]
A0A6J1E6Z0	9.5e-30	57.35	cycloeucalenol cycloisomerase-like OS=Cucurbita moschata OX=3662 GN=LOC111431284...	[more]
A0A6J1KJU7	6.2e-29	84.72	cycloeucalenol cycloisomerase-like OS=Cucurbita maxima OX=3661 GN=LOC111495263 P...	[more]
A0A5A7UM17	8.9e-28	81.69	Cycloeucalenol cycloisomerase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sc...	[more]

BLAST of Carg06791 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G50375.1	1.4e-25	71.01	cyclopropyl isomerase	[more]
AT5G50375.2	1.4e-25	71.01	cyclopropyl isomerase	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 155..155
None	No IPR available	PANTHER	PTHR35136:SF2	BNAA09G41120D PROTEIN	coord: 65..135
IPR020532	Cycloeucalenol cycloisomerase	PANTHER	PTHR35136	CYCLOEUCALENOL CYCLOISOMERASE	coord: 65..135

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg06791-RA	Carg06791-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0047793	cycloeucalenol cycloisomerase activity