Carg06771 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg06771 vs. NCBI nr
Match: KAG7012290.1 (GDSL esterase/lipase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1204.1 bits (3114), Expect = 0.0e+00
Identity = 583/583 (100.00%), Postives = 583/583 (100.00%), Query Frame = 0

Query: 1   MAYPEPASVEREVMQVNNAQRKPRILLAASGSVASIKFGNLYRSFSEWADVKAVATRAAL 60
           MAYPEPASVEREVMQVNNAQRKPRILLAASGSVASIKFGNLYRSFSEWADVKAVATRAAL
Sbjct: 1   MAYPEPASVEREVMQVNNAQRKPRILLAASGSVASIKFGNLYRSFSEWADVKAVATRAAL 60

Query: 61  HFIDRESLPKDAVLYTDEDEWTGWNKIGDSVLHIELRRWADIMVIAPLSANTLGKIAGGL 120
           HFIDRESLPKDAVLYTDEDEWTGWNKIGDSVLHIELRRWADIMVIAPLSANTLGKIAGGL
Sbjct: 61  HFIDRESLPKDAVLYTDEDEWTGWNKIGDSVLHIELRRWADIMVIAPLSANTLGKIAGGL 120

Query: 121 CDNLLTCVVRAWDYNKPLFVAPAMNPYMWTNPFTERHLIAIDELGITLIPPVTKRLACGD 180
           CDNLLTCVVRAWDYNKPLFVAPAMNPYMWTNPFTERHLIAIDELGITLIPPVTKRLACGD
Sbjct: 121 CDNLLTCVVRAWDYNKPLFVAPAMNPYMWTNPFTERHLIAIDELGITLIPPVTKRLACGD 180

Query: 181 YGNGAMAEPSLIFSTATTRSLLNPILLCILSEGNTLFRKRCAIFILLCAFASASPTANEM 240
           YGNGAMAEPSLIFSTATTRSLLNPILLCILSEGNTLFRKRCAIFILLCAFASASPTANEM
Sbjct: 181 YGNGAMAEPSLIFSTATTRSLLNPILLCILSEGNTLFRKRCAIFILLCAFASASPTANEM 240

Query: 241 HPGLFSKIYAFGDSFTDTGNTRSVSGPTVFGHVSNSPYGSTFFHHSTNRYSDGRLVIDFI 300
           HPGLFSKIYAFGDSFTDTGNTRSVSGPTVFGHVSNSPYGSTFFHHSTNRYSDGRLVIDFI
Sbjct: 241 HPGLFSKIYAFGDSFTDTGNTRSVSGPTVFGHVSNSPYGSTFFHHSTNRYSDGRLVIDFI 300

Query: 301 AQTLSLPFLTPYRYLKGNESFHGVNFAVAGSTAINHDFFVRNNLSLNITPQSIQTQLHWF 360
           AQTLSLPFLTPYRYLKGNESFHGVNFAVAGSTAINHDFFVRNNLSLNITPQSIQTQLHWF
Sbjct: 301 AQTLSLPFLTPYRYLKGNESFHGVNFAVAGSTAINHDFFVRNNLSLNITPQSIQTQLHWF 360

Query: 361 NKFLETQGHRGAAAFDDALFWVGEIGVNDYAYNTGSPIPDDTIRKLGVASVTGVLQSLLK 420
           NKFLETQGHRGAAAFDDALFWVGEIGVNDYAYNTGSPIPDDTIRKLGVASVTGVLQSLLK
Sbjct: 361 NKFLETQGHRGAAAFDDALFWVGEIGVNDYAYNTGSPIPDDTIRKLGVASVTGVLQSLLK 420

Query: 421 KGAKYMVVQGLPPSGCLPLAMSIAPVVDRDDIGCVRSSNNQTYYHNLALRASLQSLRRQF 480
           KGAKYMVVQGLPPSGCLPLAMSIAPVVDRDDIGCVRSSNNQTYYHNLALRASLQSLRRQF
Sbjct: 421 KGAKYMVVQGLPPSGCLPLAMSIAPVVDRDDIGCVRSSNNQTYYHNLALRASLQSLRRQF 480

Query: 481 PQAVIIYADYWNVYRTVMKNPSKYGFRELFKACCGVGGPYNFNVLAVCGMSSVSSCKKPY 540
           PQAVIIYADYWNVYRTVMKNPSKYGFRELFKACCGVGGPYNFNVLAVCGMSSVSSCKKPY
Sbjct: 481 PQAVIIYADYWNVYRTVMKNPSKYGFRELFKACCGVGGPYNFNVLAVCGMSSVSSCKKPY 540

Query: 541 EYINWDGVHLTEAMYKVVHDMFVEGGFTEPPFGSLFDKKRREG 584
           EYINWDGVHLTEAMYKVVHDMFVEGGFTEPPFGSLFDKKRREG
Sbjct: 541 EYINWDGVHLTEAMYKVVHDMFVEGGFTEPPFGSLFDKKRREG 583

BLAST of Carg06771 vs. NCBI nr
Match: KAG7019716.1 (GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 991.1 bits (2561), Expect = 4.0e-285
Identity = 490/594 (82.49%), Postives = 515/594 (86.70%), Query Frame = 0

Query: 1   MAYPEPASVEREVMQVNNAQRKPRILLAASGSVASIKFGNLYRSFSEWADVKAVATRAAL 60
           MAY EPA+ EREV+QVNNAQRKPRILLAASGSVASIKFGNLY SFSEWADV+AVAT+A+L
Sbjct: 48  MAYSEPANAEREVIQVNNAQRKPRILLAASGSVASIKFGNLYHSFSEWADVRAVATKASL 107

Query: 61  HFIDRESLPKDAVLYTDEDEWTGWNKIGDSVLHIELRRWADIMVIAPLSANTLGKIAGGL 120
           HFIDR SLPKD +LYTDEDEW GWNKIGDSVLHIELRRWADIMVIAPLSANTLGKIAGGL
Sbjct: 108 HFIDRASLPKDYILYTDEDEWNGWNKIGDSVLHIELRRWADIMVIAPLSANTLGKIAGGL 167

Query: 121 CDNLLTCVVRAWDYNKPLFVAPAMNPYMWTNPFTERHLIAIDELGITLIPPVTKRLACGD 180
           CDNLLTCVVRAWDYNKPLFVAPAMN +MWTNPFTERHLIAIDELGITLIPPVTKRLACGD
Sbjct: 168 CDNLLTCVVRAWDYNKPLFVAPAMNTFMWTNPFTERHLIAIDELGITLIPPVTKRLACGD 227

Query: 181 YGNGAMAEPSLIFSTA-------TTRSLLNPILLCILSEGNTLFRKRC-AIFILLCAFAS 240
           YGNGAMAEPSLIFST          +S  N +L+        +    C AIFILLC F  
Sbjct: 228 YGNGAMAEPSLIFSTVRLFYESRMQQSAKNELLIKQKKLEMMVALTSCTAIFILLCGFVL 287

Query: 241 ASPTANEMHPGLFSKIYAFGDSFTDTGNTRSVSGPTVFGHVSNSPYGSTFFHHSTNRYSD 300
           ASPTA E HP LF+KIYAFGDSFTDTGNTRSVSGP  FGHVSNSPYGSTFFHHSTNRYSD
Sbjct: 288 ASPTATETHPRLFNKIYAFGDSFTDTGNTRSVSGPAGFGHVSNSPYGSTFFHHSTNRYSD 347

Query: 301 GRLVIDFIAQTLSLPFLTPYRYLKGNESFHGVNFAVAGSTAINHDFFVRNNLSLNITPQS 360
           GRLVIDF+AQ+LSLPFL PY+YLKGN+SFHGVNFAVAGSTAINH FFVRNNLSL+ITPQS
Sbjct: 348 GRLVIDFVAQSLSLPFLPPYKYLKGNDSFHGVNFAVAGSTAINHGFFVRNNLSLDITPQS 407

Query: 361 IQTQLHWFNKFLETQGHRGA-------AAFDDALFWVGEIGVNDYAYNTGSPIPDDTIRK 420
           IQTQL WFNKFLETQ  RGA       AAFDDALFWVGEIGVNDYAY  GS I DDTIRK
Sbjct: 408 IQTQLLWFNKFLETQACRGAETKTQCKAAFDDALFWVGEIGVNDYAYAVGSSITDDTIRK 467

Query: 421 LGVASVTGVLQSLLKKGAKYMVVQGLPPSGCLPLAMSIAPVVDRDDIGCVRSSNNQTYYH 480
           LGVASVTGVLQSLLKKGAKYMVVQGLPPSGCL LAMS+APV DRDDIGCVRS NNQTY H
Sbjct: 468 LGVASVTGVLQSLLKKGAKYMVVQGLPPSGCLALAMSLAPVTDRDDIGCVRSLNNQTYVH 527

Query: 481 NLALRASLQSLRRQFPQAVIIYADYWNVYRTVMKNPSKYGFRELFKACCGVGGPYNFNVL 540
           N+AL+ASLQSLR QFPQAVIIYADYWN YR VMKNPSKYGFRE FKACCGVG PYNF++ 
Sbjct: 528 NIALQASLQSLREQFPQAVIIYADYWNAYRMVMKNPSKYGFRERFKACCGVGEPYNFDMF 587

Query: 541 AVCGMSSVSSCKKPYEYINWDGVHLTEAMYKVVHDMFVEGGFTEPPFGSLFDKK 580
            VCGM SVSSCK P EYINWDGVHLTEAMYK VHDMF++GG T P   +L D K
Sbjct: 588 TVCGMPSVSSCKNPPEYINWDGVHLTEAMYKAVHDMFIKGGLTHPALSNLLDMK 641

BLAST of Carg06771 vs. NCBI nr
Match: TYJ99222.1 (GDSL esterase/lipase [Cucumis melo var. makuwa])

HSP 1 Score: 968.4 bits (2502), Expect = 2.8e-278
Identity = 476/590 (80.68%), Postives = 507/590 (85.93%), Query Frame = 0

Query: 1   MAYPEPASVEREVMQVNNAQRKPRILLAASGSVASIKFGNLYRSFSEWADVKAVATRAAL 60
           MAYP+ A+ EREVMQVNN QRKPRILLAASGSVASIKFGNLY SF+EWADV+AVATRA+L
Sbjct: 1   MAYPKSANAEREVMQVNNVQRKPRILLAASGSVASIKFGNLYHSFAEWADVRAVATRASL 60

Query: 61  HFIDRESLPKDAVLYTDEDEWTGWNKIGDSVLHIELRRWADIMVIAPLSANTLGKIAGGL 120
           HFIDR SLPKDAVLYTDEDEWTGWNKIGDSVLHIELRRWADIMVIAPLSANTLGKIAGGL
Sbjct: 61  HFIDRASLPKDAVLYTDEDEWTGWNKIGDSVLHIELRRWADIMVIAPLSANTLGKIAGGL 120

Query: 121 CDNLLTCVVRAWDYNKPLFVAPAMNPYMWTNPFTERHLIAIDELGITLIPPVTKRLACGD 180
           CDNLLTCVVRAWDYNKPLFVAPAMN +MWTNPFTERHLIAIDELGITLIPPVTKRLACGD
Sbjct: 121 CDNLLTCVVRAWDYNKPLFVAPAMNTFMWTNPFTERHLIAIDELGITLIPPVTKRLACGD 180

Query: 181 YGNGAMAEPSLIFSTATTRSLLNPILLCILSEGNTLFRKRCAIFILLCAFASASPTANEM 240
           YGNGAMAEPSLIFST                     +  R     +  +  +ASPTA E 
Sbjct: 181 YGNGAMAEPSLIFSTV-----------------RLFYESR-----MQQSAKNASPTAIET 240

Query: 241 HPGLFSKIYAFGDSFTDTGNTRSVSGPTVFGHVSNSPYGSTFFHHSTNRYSDGRLVIDFI 300
           HP LF+KIYAFGDSFTDTGNTRS SGP  FGHVS+ PYGSTFFHH TNRYSDGRLVIDF+
Sbjct: 241 HPRLFNKIYAFGDSFTDTGNTRSASGPAGFGHVSDPPYGSTFFHHPTNRYSDGRLVIDFV 300

Query: 301 AQTLSLPFLTPYRYLKGNESFHGVNFAVAGSTAINHDFFVRNNLSLNITPQSIQTQLHWF 360
           AQ+LSLP L PY+YLKGN+SFHGVNFAVAGSTAINH+F+VRNNLS++ TPQSIQTQL WF
Sbjct: 301 AQSLSLPLLPPYKYLKGNDSFHGVNFAVAGSTAINHEFYVRNNLSIDNTPQSIQTQLLWF 360

Query: 361 NKFLETQGHRG-------AAAFDDALFWVGEIGVNDYAYNTGSPIPDDTIRKLGVASVTG 420
           NKFLETQG RG        AAFDDALFWVGEIGVNDYAY+ GSPI  DTIRKL VASVTG
Sbjct: 361 NKFLETQGCRGEETKAQCKAAFDDALFWVGEIGVNDYAYSFGSPISADTIRKLAVASVTG 420

Query: 421 VLQSLLKKGAKYMVVQGLPPSGCLPLAMSIAPVVDRDDIGCVRSSNNQTYYHNLALRASL 480
           VLQSLLKKGAKYMVVQGLPPSGCL L+MS+A V DRDDIGCVRS NNQTY H++AL+ASL
Sbjct: 421 VLQSLLKKGAKYMVVQGLPPSGCLALSMSLASVDDRDDIGCVRSLNNQTYVHSMALQASL 480

Query: 481 QSLRRQFPQAVIIYADYWNVYRTVMKNPSKYGFRELFKACCGVGGPYNFNVLAVCGMSSV 540
           QSLRRQFP+AVIIYADYWN YRTV+KNPSKYGFRE FKACCGVG PYNF +  VCGMSSV
Sbjct: 481 QSLRRQFPEAVIIYADYWNAYRTVIKNPSKYGFRERFKACCGVGEPYNFELFTVCGMSSV 540

Query: 541 SSCKKPYEYINWDGVHLTEAMYKVVHDMFVEGGFTEPPFGSLFDKKRREG 584
           SSCK P EYINWDGVHLTEAMYKVVHDM +EGGFT PPF  L D KR +G
Sbjct: 541 SSCKTPSEYINWDGVHLTEAMYKVVHDMLIEGGFTHPPFSYLLDMKRHKG 568

BLAST of Carg06771 vs. NCBI nr
Match: KAA0055298.1 (GDSL esterase/lipase [Cucumis melo var. makuwa])

HSP 1 Score: 968.0 bits (2501), Expect = 3.7e-278
Identity = 475/590 (80.51%), Postives = 506/590 (85.76%), Query Frame = 0

Query: 1   MAYPEPASVEREVMQVNNAQRKPRILLAASGSVASIKFGNLYRSFSEWADVKAVATRAAL 60
           MAYP+ A+ EREVMQVNN QRKPRILLAASGSVASIKFGNLY SF+EWADV+AVATRA+L
Sbjct: 1   MAYPKSANAEREVMQVNNVQRKPRILLAASGSVASIKFGNLYHSFAEWADVRAVATRASL 60

Query: 61  HFIDRESLPKDAVLYTDEDEWTGWNKIGDSVLHIELRRWADIMVIAPLSANTLGKIAGGL 120
           HFIDR SLPKDAVLYTDEDEWTGWNKIGDSVLHIELRRWADIMVIAPLSANTLGKIAGGL
Sbjct: 61  HFIDRASLPKDAVLYTDEDEWTGWNKIGDSVLHIELRRWADIMVIAPLSANTLGKIAGGL 120

Query: 121 CDNLLTCVVRAWDYNKPLFVAPAMNPYMWTNPFTERHLIAIDELGITLIPPVTKRLACGD 180
           CDNLLTCVVRAWDYNKPLFVAPAMN +MWTNPFTERHLIAIDELGITLIPPVTKRLACGD
Sbjct: 121 CDNLLTCVVRAWDYNKPLFVAPAMNTFMWTNPFTERHLIAIDELGITLIPPVTKRLACGD 180

Query: 181 YGNGAMAEPSLIFSTATTRSLLNPILLCILSEGNTLFRKRCAIFILLCAFASASPTANEM 240
           YGNGAMAEPSLIFST                     +  R     +  +  +ASPTA E 
Sbjct: 181 YGNGAMAEPSLIFSTV-----------------RLFYESR-----MQQSAKNASPTATET 240

Query: 241 HPGLFSKIYAFGDSFTDTGNTRSVSGPTVFGHVSNSPYGSTFFHHSTNRYSDGRLVIDFI 300
           HP LF+KIYAFGDSFTDTGNTRS SGP  FGHVS+ PYGSTFFHH TNRYSDGRLVIDF+
Sbjct: 241 HPRLFNKIYAFGDSFTDTGNTRSASGPAGFGHVSDPPYGSTFFHHPTNRYSDGRLVIDFV 300

Query: 301 AQTLSLPFLTPYRYLKGNESFHGVNFAVAGSTAINHDFFVRNNLSLNITPQSIQTQLHWF 360
           AQ+LSLP L PY+YLKGN+SFHGVNFAVAGSTAINH+F+VRNNLS++ TPQSIQTQL WF
Sbjct: 301 AQSLSLPLLPPYKYLKGNDSFHGVNFAVAGSTAINHEFYVRNNLSIDNTPQSIQTQLLWF 360

Query: 361 NKFLETQGHRGA-------AAFDDALFWVGEIGVNDYAYNTGSPIPDDTIRKLGVASVTG 420
           NKFLETQG RG        AAFDDALFWVGEIGVNDYAY+ GSPI  DTIRKL VASVTG
Sbjct: 361 NKFLETQGCRGEETKAQCNAAFDDALFWVGEIGVNDYAYSFGSPISADTIRKLAVASVTG 420

Query: 421 VLQSLLKKGAKYMVVQGLPPSGCLPLAMSIAPVVDRDDIGCVRSSNNQTYYHNLALRASL 480
           VLQSLLKKGAKYMVVQGLPPSGCL L+MS+A V DRDDIGCVRS NNQTY H++AL+ASL
Sbjct: 421 VLQSLLKKGAKYMVVQGLPPSGCLALSMSLASVDDRDDIGCVRSLNNQTYVHSMALQASL 480

Query: 481 QSLRRQFPQAVIIYADYWNVYRTVMKNPSKYGFRELFKACCGVGGPYNFNVLAVCGMSSV 540
           QSLRRQFP+A IIYADYWN YRTV+KNPSKYGFRE FKACCGVG PYNF +  VCGMSSV
Sbjct: 481 QSLRRQFPEAAIIYADYWNAYRTVIKNPSKYGFRERFKACCGVGEPYNFELFTVCGMSSV 540

Query: 541 SSCKKPYEYINWDGVHLTEAMYKVVHDMFVEGGFTEPPFGSLFDKKRREG 584
           SSCK P EYINWDGVHLTEAMYKVVHDM +EGGFT PPF  L D KR +G
Sbjct: 541 SSCKTPSEYINWDGVHLTEAMYKVVHDMLIEGGFTHPPFSYLLDMKRHKG 568

BLAST of Carg06771 vs. NCBI nr
Match: KAG6573104.1 (GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 746.5 bits (1926), Expect = 1.7e-211
Identity = 358/362 (98.90%), Postives = 359/362 (99.17%), Query Frame = 0

Query: 222 AIFILLCAFASASPTANEMHPGLFSKIYAFGDSFTDTGNTRSVSGPTVFGHVSNSPYGST 281
           AIFILLCAFASASPTANE HP LFSKIYAFGDSFTDTGNTRSVSGPTVFGHVSNSPYGST
Sbjct: 15  AIFILLCAFASASPTANETHPHLFSKIYAFGDSFTDTGNTRSVSGPTVFGHVSNSPYGST 74

Query: 282 FFHHSTNRYSDGRLVIDFIAQTLSLPFLTPYRYLKGNESFHGVNFAVAGSTAINHDFFVR 341
           FFHHSTNRYSDGRLVIDFIAQTLSLPFLTPYRYLKGNESFHGVNFAVAGSTAINHDFFVR
Sbjct: 75  FFHHSTNRYSDGRLVIDFIAQTLSLPFLTPYRYLKGNESFHGVNFAVAGSTAINHDFFVR 134

Query: 342 NNLSLNITPQSIQTQLHWFNKFLETQGHRGAAAFDDALFWVGEIGVNDYAYNTGSPIPDD 401
           NNLSLNITPQSIQTQLHWFNKFLETQGHRGAAAFDDALFWVGEIGVNDYAYNTGSPIPDD
Sbjct: 135 NNLSLNITPQSIQTQLHWFNKFLETQGHRGAAAFDDALFWVGEIGVNDYAYNTGSPIPDD 194

Query: 402 TIRKLGVASVTGVLQSLLKKGAKYMVVQGLPPSGCLPLAMSIAPVVDRDDIGCVRSSNNQ 461
           TIRKLGVASVTGVLQSLLKKGAKYMVVQGLPPSGCLPLAMSIAPVVDRDDIGCVRSSNNQ
Sbjct: 195 TIRKLGVASVTGVLQSLLKKGAKYMVVQGLPPSGCLPLAMSIAPVVDRDDIGCVRSSNNQ 254

Query: 462 TYYHNLALRASLQSLRRQFPQAVIIYADYWNVYRTVMKNPSKYGFRELFKACCGVGGPYN 521
           TYYHNLALRASLQSLRRQFPQAVIIYADYWNVYRTVMKNP KYGFRELFKACCG+GGPYN
Sbjct: 255 TYYHNLALRASLQSLRRQFPQAVIIYADYWNVYRTVMKNPGKYGFRELFKACCGIGGPYN 314

Query: 522 FNVLAVCGMSSVSSCKKPYEYINWDGVHLTEAMYKVVHDMFVEGGFTEPPFGSLFDKKRR 581
           FNVLAVCGMSSVSSCKKPYEYINWDGVHLTEAMYKVVHDMFVEGGFTEPPFGSLFDKKRR
Sbjct: 315 FNVLAVCGMSSVSSCKKPYEYINWDGVHLTEAMYKVVHDMFVEGGFTEPPFGSLFDKKRR 374

Query: 582 EG 584
           EG
Sbjct: 375 EG 376

BLAST of Carg06771 vs. ExPASy Swiss-Prot
Match: Q9STM6 (GDSL esterase/lipase At3g48460 OS=Arabidopsis thaliana OX=3702 GN=At3g48460 PE=2 SV=1)

HSP 1 Score: 451.1 bits (1159), Expect = 2.0e-125
Identity = 215/365 (58.90%), Postives = 271/365 (74.25%), Query Frame = 0

Query: 222 AIFILLCAFASASPTANEMHPGLFSKIYAFGDSFTDTGNTRSVSGPTVFGHVSNSPYGST 281
           ++ ILL +  S + T   +H   F+KIYAFGDSFTDTGN+RS  GP  FGH+S+ PYG T
Sbjct: 14  SVAILLFSTISTAATIPNIHRP-FNKIYAFGDSFTDTGNSRSGEGPAGFGHLSSPPYGMT 73

Query: 282 FFHHSTNRYSDGRLVIDFIAQTLSLPFLTPYRYLKGNES------FHGVNFAVAGSTAIN 341
           FF   TNRYSDGRL IDF+A++++LPFL PY  LK   +       HGVNFAV+GST I 
Sbjct: 74  FFRRPTNRYSDGRLTIDFVAESMNLPFLPPYLSLKTTNANGTATDTHGVNFAVSGSTVIK 133

Query: 342 HDFFVRNNLSLNITPQSIQTQLHWFNKFLETQG-HRGAAAFDDALFWVGEIGVNDYAYNT 401
           H FFV+NNLSL++TPQSI+T+L WF K+LET G ++  + F D+LFW+GEIGVNDYAY  
Sbjct: 134 HAFFVKNNLSLDMTPQSIETELAWFEKYLETLGTNQKVSLFKDSLFWIGEIGVNDYAYTL 193

Query: 402 GSPIPDDTIRKLGVASVTGVLQSLLKKGAKYMVVQGLPPSGCLPLAMSIAPVVDRDDIGC 461
           GS +  DTIR+L +++ T  L++LL KG KYM+VQG P +GCL LAMS+A   DRD +GC
Sbjct: 194 GSTVSSDTIRELSISTFTRFLETLLNKGVKYMLVQGHPATGCLTLAMSLAAEDDRDSLGC 253

Query: 462 VRSSNNQTYYHNLALRASLQSLRRQFPQAVIIYADYWNVYRTVMKNPSKYGFRELFKACC 521
           V+S+NNQ+Y HNLAL++ L+ LR ++P A I+YADYWN YR V+K+PSKYG  E FKACC
Sbjct: 254 VQSANNQSYTHNLALQSKLKQLRIKYPSATIVYADYWNAYRAVIKHPSKYGITEKFKACC 313

Query: 522 GVGGPYNFNVLAVCGMSSVSSCKKPYEYINWDGVHLTEAMYKVVHDMFVEGGFTEPPFGS 580
           G+G PYNF V   CG  + + CK P +YINWDGVHLTEAMYKV+ DMF++G FT P F  
Sbjct: 314 GIGEPYNFQVFQTCGTDAATVCKDPNQYINWDGVHLTEAMYKVMADMFLDGTFTRPRFSD 373

BLAST of Carg06771 vs. ExPASy Swiss-Prot
Match: Q9SWE5 (Phosphopantothenoylcysteine decarboxylase OS=Arabidopsis thaliana OX=3702 GN=HAL3A PE=1 SV=1)

HSP 1 Score: 320.5 bits (820), Expect = 4.0e-86
Identity = 143/186 (76.88%), Postives = 168/186 (90.32%), Query Frame = 0

Query: 10  EREVMQVNNAQRKPRILLAASGSVASIKFGNLYRSFSEWADVKAVATRAALHFIDRESLP 69
           +R+ M+VN   RKPR+LLAASGSVA+IKFGNL   F+EWA+V+AV T+++LHF+D+ SLP
Sbjct: 7   DRQDMEVNTTPRKPRVLLAASGSVAAIKFGNLCHCFTEWAEVRAVVTKSSLHFLDKLSLP 66

Query: 70  KDAVLYTDEDEWTGWNKIGDSVLHIELRRWADIMVIAPLSANTLGKIAGGLCDNLLTCVV 129
           ++  LYTDEDEW+ WNKIGD VLHIELRRWAD++VIAPLSANTLGKIAGGLCDNLLTC++
Sbjct: 67  QEVTLYTDEDEWSSWNKIGDPVLHIELRRWADVLVIAPLSANTLGKIAGGLCDNLLTCII 126

Query: 130 RAWDYNKPLFVAPAMNPYMWTNPFTERHLIAIDELGITLIPPVTKRLACGDYGNGAMAEP 189
           RAWDY KPLFVAPAMN  MW NPFTERHL+++DELGITLIPP+ KRLACGDYGNGAMAEP
Sbjct: 127 RAWDYTKPLFVAPAMNTLMWNNPFTERHLLSLDELGITLIPPIKKRLACGDYGNGAMAEP 186

Query: 190 SLIFST 196
           SLI+ST
Sbjct: 187 SLIYST 192

BLAST of Carg06771 vs. ExPASy Swiss-Prot
Match: P94063 (Probable phosphopantothenoylcysteine decarboxylase OS=Arabidopsis thaliana OX=3702 GN=HAL3B PE=2 SV=2)

HSP 1 Score: 313.9 bits (803), Expect = 3.8e-84
Identity = 142/182 (78.02%), Postives = 164/182 (90.11%), Query Frame = 0

Query: 14  MQVNNAQRKPRILLAASGSVASIKFGNLYRSFSEWADVKAVATRAALHFIDRESLPKDAV 73
           M+V+   RKPRILLAASGSVASIKF NL   FSEWA+VKAVA++++L+F+D+ SLP++  
Sbjct: 3   MEVDTVTRKPRILLAASGSVASIKFSNLCHCFSEWAEVKAVASKSSLNFVDKPSLPQNVT 62

Query: 74  LYTDEDEWTGWNKIGDSVLHIELRRWADIMVIAPLSANTLGKIAGGLCDNLLTCVVRAWD 133
           LYTDEDEW+ WNKIGD VLHIELRRWAD+M+IAPLSANTL KIAGGLCDNLLTC+VRAWD
Sbjct: 63  LYTDEDEWSSWNKIGDPVLHIELRRWADVMIIAPLSANTLAKIAGGLCDNLLTCIVRAWD 122

Query: 134 YNKPLFVAPAMNPYMWTNPFTERHLIAIDELGITLIPPVTKRLACGDYGNGAMAEPSLIF 193
           Y+KPLFVAPAMN  MW NPFTERHL+ +DELGITLIPP+ K+LACGDYGNGAMAEPSLI+
Sbjct: 123 YSKPLFVAPAMNTLMWNNPFTERHLVLLDELGITLIPPIKKKLACGDYGNGAMAEPSLIY 182

Query: 194 ST 196
           ST
Sbjct: 183 ST 184

BLAST of Carg06771 vs. ExPASy Swiss-Prot
Match: Q69K55 (Phosphopantothenoylcysteine decarboxylase OS=Oryza sativa subsp. japonica OX=39947 GN=HAL3 PE=1 SV=1)

HSP 1 Score: 300.8 bits (769), Expect = 3.3e-80
Identity = 139/184 (75.54%), Postives = 160/184 (86.96%), Query Frame = 0

Query: 22  KPRILLAASGSVASIKFGNLYRSFSEWADVKAVATRAALHFIDRESLPKDAVLYTDEDEW 81
           KPR+LLAASGSVA+IKF +L RSFSEWA+V+AVAT+A+LHFIDR SLP + +LYTD+DEW
Sbjct: 20  KPRVLLAASGSVAAIKFESLCRSFSEWAEVRAVATKASLHFIDRTSLPSNIILYTDDDEW 79

Query: 82  TGWNKIGDSVLHIELRRWADIMVIAPLSANTLGKIAGGLCDNLLTCVVRAWDYNKPLFVA 141
           + W KIGD VLHIELR+WADIMVIAPLSANTL KIAGGLCDNLLTC+VRAWDY+KPLFVA
Sbjct: 80  STWKKIGDEVLHIELRKWADIMVIAPLSANTLAKIAGGLCDNLLTCIVRAWDYSKPLFVA 139

Query: 142 PAMNPYMWTNPFTERHLIAIDELGITLIPPVTKRLACGDYGNGAMAEPSLIFSTATTRSL 201
           PAMN +MW NPFT RHL  I+ LGI+L+PP+TKRLACGDYGNGAMAEPS+I ST      
Sbjct: 140 PAMNTFMWNNPFTSRHLETINLLGISLVPPITKRLACGDYGNGAMAEPSVIDSTVRLACK 199

Query: 202 LNPI 206
             P+
Sbjct: 200 RQPL 203

BLAST of Carg06771 vs. ExPASy Swiss-Prot
Match: Q94F40 (GDSL esterase/lipase At1g28600 OS=Arabidopsis thaliana OX=3702 GN=At1g28600 PE=2 SV=1)

HSP 1 Score: 240.7 bits (613), Expect = 4.0e-62
Identity = 132/339 (38.94%), Postives = 185/339 (54.57%), Query Frame = 0

Query: 245 FSKIYAFGDSFTDTGNTRSVSGPTVFGHVSNSPYGSTFFHHSTNRYSDGRLVIDFIAQTL 304
           F  I +FGDS  DTGN   +S        +  PYG TFFHH T R  DGR+++DFIA+ +
Sbjct: 29  FKSIISFGDSIADTGNLVGLSDRNQLPVTAFPPYGETFFHHPTGRSCDGRIIMDFIAEFV 88

Query: 305 SLPFLTPYRYLKGNESFHGVNFAVAGSTAINHDFFVRNNLSLNITPQSIQTQLHWFNKFL 364
            LP++ PY   K      GVNFAVAG+TA+   F  +  +  + T  S+  QL  F K L
Sbjct: 89  GLPYVPPYFGSKNRNFDKGVNFAVAGATALKSSFLKKRGIQPH-TNVSLGVQLKSFKKSL 148

Query: 365 ETQGHRGAAAFD---DALFWVGEIGVNDYAYNTGSPIPDDTIRKL---GVASVTGVLQSL 424
                  +   D   +AL  +GEIG NDY +   +  P   + +L    +AS++  +  L
Sbjct: 149 PNLCGSPSDCRDMIGNALILMGEIGGNDYNFPFFNRKPVKEVEELVPFVIASISSTITEL 208

Query: 425 LKKGAKYMVVQGLPPSGCLPLAMSIAPVVDRDD----IGCVRSSNNQTYYHNLALRASLQ 484
           +  G K  +V G  P GC  + +++    ++D+     GC++  N    YH+  L+  L 
Sbjct: 209 IGMGGKTFLVPGEFPIGCSVVYLTLYKTSNKDEYDPSTGCLKWLNKFGEYHSEKLKVELN 268

Query: 485 SLRRQFPQAVIIYADYWNVYRTVMKNPSKYGFREL-FKACCGVGGPYNFNVLAVCGMSSV 544
            LR+ +P   IIYADY+N    + K P+K+GF E  F ACCG+GGPYNFN    CG   V
Sbjct: 269 RLRKLYPHVNIIYADYYNSLLRIFKEPAKFGFMERPFPACCGIGGPYNFNFTRKCGSVGV 328

Query: 545 SSCKKPYEYINWDGVHLTEAMYKVVHDMFVEGGFTEPPF 573
            SCK P +Y+ WDGVH+TEA YK + D  + G +  PPF
Sbjct: 329 KSCKDPSKYVGWDGVHMTEAAYKWIADGILNGPYANPPF 366

BLAST of Carg06771 vs. ExPASy TrEMBL
Match: A0A5D3BHJ8 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004330 PE=3 SV=1)

HSP 1 Score: 968.4 bits (2502), Expect = 1.4e-278
Identity = 476/590 (80.68%), Postives = 507/590 (85.93%), Query Frame = 0

Query: 1   MAYPEPASVEREVMQVNNAQRKPRILLAASGSVASIKFGNLYRSFSEWADVKAVATRAAL 60
           MAYP+ A+ EREVMQVNN QRKPRILLAASGSVASIKFGNLY SF+EWADV+AVATRA+L
Sbjct: 1   MAYPKSANAEREVMQVNNVQRKPRILLAASGSVASIKFGNLYHSFAEWADVRAVATRASL 60

Query: 61  HFIDRESLPKDAVLYTDEDEWTGWNKIGDSVLHIELRRWADIMVIAPLSANTLGKIAGGL 120
           HFIDR SLPKDAVLYTDEDEWTGWNKIGDSVLHIELRRWADIMVIAPLSANTLGKIAGGL
Sbjct: 61  HFIDRASLPKDAVLYTDEDEWTGWNKIGDSVLHIELRRWADIMVIAPLSANTLGKIAGGL 120

Query: 121 CDNLLTCVVRAWDYNKPLFVAPAMNPYMWTNPFTERHLIAIDELGITLIPPVTKRLACGD 180
           CDNLLTCVVRAWDYNKPLFVAPAMN +MWTNPFTERHLIAIDELGITLIPPVTKRLACGD
Sbjct: 121 CDNLLTCVVRAWDYNKPLFVAPAMNTFMWTNPFTERHLIAIDELGITLIPPVTKRLACGD 180

Query: 181 YGNGAMAEPSLIFSTATTRSLLNPILLCILSEGNTLFRKRCAIFILLCAFASASPTANEM 240
           YGNGAMAEPSLIFST                     +  R     +  +  +ASPTA E 
Sbjct: 181 YGNGAMAEPSLIFSTV-----------------RLFYESR-----MQQSAKNASPTAIET 240

Query: 241 HPGLFSKIYAFGDSFTDTGNTRSVSGPTVFGHVSNSPYGSTFFHHSTNRYSDGRLVIDFI 300
           HP LF+KIYAFGDSFTDTGNTRS SGP  FGHVS+ PYGSTFFHH TNRYSDGRLVIDF+
Sbjct: 241 HPRLFNKIYAFGDSFTDTGNTRSASGPAGFGHVSDPPYGSTFFHHPTNRYSDGRLVIDFV 300

Query: 301 AQTLSLPFLTPYRYLKGNESFHGVNFAVAGSTAINHDFFVRNNLSLNITPQSIQTQLHWF 360
           AQ+LSLP L PY+YLKGN+SFHGVNFAVAGSTAINH+F+VRNNLS++ TPQSIQTQL WF
Sbjct: 301 AQSLSLPLLPPYKYLKGNDSFHGVNFAVAGSTAINHEFYVRNNLSIDNTPQSIQTQLLWF 360

Query: 361 NKFLETQGHRG-------AAAFDDALFWVGEIGVNDYAYNTGSPIPDDTIRKLGVASVTG 420
           NKFLETQG RG        AAFDDALFWVGEIGVNDYAY+ GSPI  DTIRKL VASVTG
Sbjct: 361 NKFLETQGCRGEETKAQCKAAFDDALFWVGEIGVNDYAYSFGSPISADTIRKLAVASVTG 420

Query: 421 VLQSLLKKGAKYMVVQGLPPSGCLPLAMSIAPVVDRDDIGCVRSSNNQTYYHNLALRASL 480
           VLQSLLKKGAKYMVVQGLPPSGCL L+MS+A V DRDDIGCVRS NNQTY H++AL+ASL
Sbjct: 421 VLQSLLKKGAKYMVVQGLPPSGCLALSMSLASVDDRDDIGCVRSLNNQTYVHSMALQASL 480

Query: 481 QSLRRQFPQAVIIYADYWNVYRTVMKNPSKYGFRELFKACCGVGGPYNFNVLAVCGMSSV 540
           QSLRRQFP+AVIIYADYWN YRTV+KNPSKYGFRE FKACCGVG PYNF +  VCGMSSV
Sbjct: 481 QSLRRQFPEAVIIYADYWNAYRTVIKNPSKYGFRERFKACCGVGEPYNFELFTVCGMSSV 540

Query: 541 SSCKKPYEYINWDGVHLTEAMYKVVHDMFVEGGFTEPPFGSLFDKKRREG 584
           SSCK P EYINWDGVHLTEAMYKVVHDM +EGGFT PPF  L D KR +G
Sbjct: 541 SSCKTPSEYINWDGVHLTEAMYKVVHDMLIEGGFTHPPFSYLLDMKRHKG 568

BLAST of Carg06771 vs. ExPASy TrEMBL
Match: A0A5A7UJV8 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold80G001410 PE=3 SV=1)

HSP 1 Score: 968.0 bits (2501), Expect = 1.8e-278
Identity = 475/590 (80.51%), Postives = 506/590 (85.76%), Query Frame = 0

Query: 1   MAYPEPASVEREVMQVNNAQRKPRILLAASGSVASIKFGNLYRSFSEWADVKAVATRAAL 60
           MAYP+ A+ EREVMQVNN QRKPRILLAASGSVASIKFGNLY SF+EWADV+AVATRA+L
Sbjct: 1   MAYPKSANAEREVMQVNNVQRKPRILLAASGSVASIKFGNLYHSFAEWADVRAVATRASL 60

Query: 61  HFIDRESLPKDAVLYTDEDEWTGWNKIGDSVLHIELRRWADIMVIAPLSANTLGKIAGGL 120
           HFIDR SLPKDAVLYTDEDEWTGWNKIGDSVLHIELRRWADIMVIAPLSANTLGKIAGGL
Sbjct: 61  HFIDRASLPKDAVLYTDEDEWTGWNKIGDSVLHIELRRWADIMVIAPLSANTLGKIAGGL 120

Query: 121 CDNLLTCVVRAWDYNKPLFVAPAMNPYMWTNPFTERHLIAIDELGITLIPPVTKRLACGD 180
           CDNLLTCVVRAWDYNKPLFVAPAMN +MWTNPFTERHLIAIDELGITLIPPVTKRLACGD
Sbjct: 121 CDNLLTCVVRAWDYNKPLFVAPAMNTFMWTNPFTERHLIAIDELGITLIPPVTKRLACGD 180

Query: 181 YGNGAMAEPSLIFSTATTRSLLNPILLCILSEGNTLFRKRCAIFILLCAFASASPTANEM 240
           YGNGAMAEPSLIFST                     +  R     +  +  +ASPTA E 
Sbjct: 181 YGNGAMAEPSLIFSTV-----------------RLFYESR-----MQQSAKNASPTATET 240

Query: 241 HPGLFSKIYAFGDSFTDTGNTRSVSGPTVFGHVSNSPYGSTFFHHSTNRYSDGRLVIDFI 300
           HP LF+KIYAFGDSFTDTGNTRS SGP  FGHVS+ PYGSTFFHH TNRYSDGRLVIDF+
Sbjct: 241 HPRLFNKIYAFGDSFTDTGNTRSASGPAGFGHVSDPPYGSTFFHHPTNRYSDGRLVIDFV 300

Query: 301 AQTLSLPFLTPYRYLKGNESFHGVNFAVAGSTAINHDFFVRNNLSLNITPQSIQTQLHWF 360
           AQ+LSLP L PY+YLKGN+SFHGVNFAVAGSTAINH+F+VRNNLS++ TPQSIQTQL WF
Sbjct: 301 AQSLSLPLLPPYKYLKGNDSFHGVNFAVAGSTAINHEFYVRNNLSIDNTPQSIQTQLLWF 360

Query: 361 NKFLETQGHRGA-------AAFDDALFWVGEIGVNDYAYNTGSPIPDDTIRKLGVASVTG 420
           NKFLETQG RG        AAFDDALFWVGEIGVNDYAY+ GSPI  DTIRKL VASVTG
Sbjct: 361 NKFLETQGCRGEETKAQCNAAFDDALFWVGEIGVNDYAYSFGSPISADTIRKLAVASVTG 420

Query: 421 VLQSLLKKGAKYMVVQGLPPSGCLPLAMSIAPVVDRDDIGCVRSSNNQTYYHNLALRASL 480
           VLQSLLKKGAKYMVVQGLPPSGCL L+MS+A V DRDDIGCVRS NNQTY H++AL+ASL
Sbjct: 421 VLQSLLKKGAKYMVVQGLPPSGCLALSMSLASVDDRDDIGCVRSLNNQTYVHSMALQASL 480

Query: 481 QSLRRQFPQAVIIYADYWNVYRTVMKNPSKYGFRELFKACCGVGGPYNFNVLAVCGMSSV 540
           QSLRRQFP+A IIYADYWN YRTV+KNPSKYGFRE FKACCGVG PYNF +  VCGMSSV
Sbjct: 481 QSLRRQFPEAAIIYADYWNAYRTVIKNPSKYGFRERFKACCGVGEPYNFELFTVCGMSSV 540

Query: 541 SSCKKPYEYINWDGVHLTEAMYKVVHDMFVEGGFTEPPFGSLFDKKRREG 584
           SSCK P EYINWDGVHLTEAMYKVVHDM +EGGFT PPF  L D KR +G
Sbjct: 541 SSCKTPSEYINWDGVHLTEAMYKVVHDMLIEGGFTHPPFSYLLDMKRHKG 568

BLAST of Carg06771 vs. ExPASy TrEMBL
Match: A0A6J1GS63 (GDSL esterase/lipase At3g48460-like OS=Cucurbita moschata OX=3662 GN=LOC111456968 PE=3 SV=1)

HSP 1 Score: 732.6 bits (1890), Expect = 1.3e-207
Identity = 352/362 (97.24%), Postives = 354/362 (97.79%), Query Frame = 0

Query: 222 AIFILLCAFASASPTANEMHPGLFSKIYAFGDSFTDTGNTRSVSGPTVFGHVSNSPYGST 281
           AIFILL AFASASPTANE HP LFSKIYAFGDSFTDTGNTRSVSGPTVFGHVSNSPYGST
Sbjct: 15  AIFILLYAFASASPTANETHPRLFSKIYAFGDSFTDTGNTRSVSGPTVFGHVSNSPYGST 74

Query: 282 FFHHSTNRYSDGRLVIDFIAQTLSLPFLTPYRYLKGNESFHGVNFAVAGSTAINHDFFVR 341
           FFHHSTNRYSDGRLVIDFIAQTLSLPFLTPYRYLKGNESFHGVNFAVAGSTAINHDFFVR
Sbjct: 75  FFHHSTNRYSDGRLVIDFIAQTLSLPFLTPYRYLKGNESFHGVNFAVAGSTAINHDFFVR 134

Query: 342 NNLSLNITPQSIQTQLHWFNKFLETQGHRGAAAFDDALFWVGEIGVNDYAYNTGSPIPDD 401
           NNLSLNITPQSIQTQLHWFNKFLETQGHRGAAAFDDALFWVGEIGVNDYAYNTGSPIPDD
Sbjct: 135 NNLSLNITPQSIQTQLHWFNKFLETQGHRGAAAFDDALFWVGEIGVNDYAYNTGSPIPDD 194

Query: 402 TIRKLGVASVTGVLQSLLKKGAKYMVVQGLPPSGCLPLAMSIAPVVDRDDIGCVRSSNNQ 461
           TI KLGVAS+TGVLQSLLKKGAKYMVVQGLPPSGCLPLAMSIAPVVDRDDIGCVRS NNQ
Sbjct: 195 TILKLGVASITGVLQSLLKKGAKYMVVQGLPPSGCLPLAMSIAPVVDRDDIGCVRSLNNQ 254

Query: 462 TYYHNLALRASLQSLRRQFPQAVIIYADYWNVYRTVMKNPSKYGFRELFKACCGVGGPYN 521
           TYYHNLALRASLQSLRRQFPQAVIIYADYWN YRTVMKNP KYGFRELF+ACCGVGGPYN
Sbjct: 255 TYYHNLALRASLQSLRRQFPQAVIIYADYWNAYRTVMKNPGKYGFRELFRACCGVGGPYN 314

Query: 522 FNVLAVCGMSSVSSCKKPYEYINWDGVHLTEAMYKVVHDMFVEGGFTEPPFGSLFDKKRR 581
           FNVLAVCGMSSVSSCKKPYEYINWDGVHLTEAMYKVVHDMFVEGGFTEPPFGSLFDKKR 
Sbjct: 315 FNVLAVCGMSSVSSCKKPYEYINWDGVHLTEAMYKVVHDMFVEGGFTEPPFGSLFDKKRH 374

Query: 582 EG 584
           EG
Sbjct: 375 EG 376

BLAST of Carg06771 vs. ExPASy TrEMBL
Match: A0A6J1K249 (GDSL esterase/lipase At3g48460-like OS=Cucurbita maxima OX=3661 GN=LOC111489982 PE=3 SV=1)

HSP 1 Score: 730.3 bits (1884), Expect = 6.2e-207
Identity = 348/362 (96.13%), Postives = 354/362 (97.79%), Query Frame = 0

Query: 222 AIFILLCAFASASPTANEMHPGLFSKIYAFGDSFTDTGNTRSVSGPTVFGHVSNSPYGST 281
           AIFI+LCAFASASPTANE HP LFSKIYAFGDSFTDTGNTRSVSGPTVFGHVSNSPYGST
Sbjct: 9   AIFIVLCAFASASPTANETHPRLFSKIYAFGDSFTDTGNTRSVSGPTVFGHVSNSPYGST 68

Query: 282 FFHHSTNRYSDGRLVIDFIAQTLSLPFLTPYRYLKGNESFHGVNFAVAGSTAINHDFFVR 341
           FFHHSTNRYSDGRLVIDF+AQTLSLPFLTPYRYLKGNESFHGVNFAVAGSTAINHDFFVR
Sbjct: 69  FFHHSTNRYSDGRLVIDFVAQTLSLPFLTPYRYLKGNESFHGVNFAVAGSTAINHDFFVR 128

Query: 342 NNLSLNITPQSIQTQLHWFNKFLETQGHRGAAAFDDALFWVGEIGVNDYAYNTGSPIPDD 401
           NNLSLNITPQSIQTQL WFNKFLETQGHRGAAAFDDALFWVGEIGVNDYAYNTGSPIPDD
Sbjct: 129 NNLSLNITPQSIQTQLLWFNKFLETQGHRGAAAFDDALFWVGEIGVNDYAYNTGSPIPDD 188

Query: 402 TIRKLGVASVTGVLQSLLKKGAKYMVVQGLPPSGCLPLAMSIAPVVDRDDIGCVRSSNNQ 461
           TIRKLGVASVTGVLQSLLKKGAKYMVVQGLPPSGCLPLAMS+APV DRDDIGCVRSSNNQ
Sbjct: 189 TIRKLGVASVTGVLQSLLKKGAKYMVVQGLPPSGCLPLAMSVAPVTDRDDIGCVRSSNNQ 248

Query: 462 TYYHNLALRASLQSLRRQFPQAVIIYADYWNVYRTVMKNPSKYGFRELFKACCGVGGPYN 521
           TYYHNLALRASLQSLRRQFPQAVI+YADYWN Y+TVMKNP KYGFRE FKACCGVGGPYN
Sbjct: 249 TYYHNLALRASLQSLRRQFPQAVIVYADYWNAYQTVMKNPGKYGFRESFKACCGVGGPYN 308

Query: 522 FNVLAVCGMSSVSSCKKPYEYINWDGVHLTEAMYKVVHDMFVEGGFTEPPFGSLFDKKRR 581
           FNVLAVCGMSSVSSCKKPYEYINWDGVHLTEAMYKVVHDMFVEGGFT+PPFGSLFDKKR 
Sbjct: 309 FNVLAVCGMSSVSSCKKPYEYINWDGVHLTEAMYKVVHDMFVEGGFTQPPFGSLFDKKRH 368

Query: 582 EG 584
           EG
Sbjct: 369 EG 370

BLAST of Carg06771 vs. ExPASy TrEMBL
Match: A0A6J1C9C3 (GDSL esterase/lipase At3g48460 OS=Momordica charantia OX=3673 GN=LOC111008661 PE=3 SV=1)

HSP 1 Score: 641.0 bits (1652), Expect = 5.0e-180
Identity = 307/369 (83.20%), Postives = 330/369 (89.43%), Query Frame = 0

Query: 222 AIFILLCAFASASPTANEMHPGLFSKIYAFGDSFTDTGNTRSVSGPTVFGHVSNSPYGST 281
           A+++LLCAFASASPTA E HP LF+KIYAFGDSFTDTGNTRS+SGPT FGHVS+ PYGST
Sbjct: 11  AVYVLLCAFASASPTATETHPRLFNKIYAFGDSFTDTGNTRSMSGPTGFGHVSSPPYGST 70

Query: 282 FFHHSTNRYSDGRLVIDFIAQTLSLPFLTPYRYLKGNESFHGVNFAVAGSTAINHDFFVR 341
           FFHH TNRYSDGRLVIDF+A+TLSLPFL PY+YLKGN+SFHGVNFAVAGSTAINHDFFVR
Sbjct: 71  FFHHPTNRYSDGRLVIDFVAETLSLPFLPPYKYLKGNDSFHGVNFAVAGSTAINHDFFVR 130

Query: 342 NNLSLNITPQSIQTQLHWFNKFLETQGHRGA-------AAFDDALFWVGEIGVNDYAYNT 401
           NNLSL+ITPQSIQTQL WFN+FLETQG RGA       AA DDALFWVGEIGVNDYAY+ 
Sbjct: 131 NNLSLDITPQSIQTQLLWFNEFLETQGCRGAETEKQCKAALDDALFWVGEIGVNDYAYDI 190

Query: 402 GSPIPDDTIRKLGVASVTGVLQSLLKKGAKYMVVQGLPPSGCLPLAMSIAPVVDRDDIGC 461
           GSPIPDDTIRKLGVASVTG LQSLLKKGAKY+VVQGLPPSGCL LAMS+APV DRDDIGC
Sbjct: 191 GSPIPDDTIRKLGVASVTGFLQSLLKKGAKYVVVQGLPPSGCLALAMSLAPVTDRDDIGC 250

Query: 462 VRSSNNQTYYHNLALRASLQSLRRQFPQAVIIYADYWNVYRTVMKNPSKYGFRELFKACC 521
           VRS +NQTY HN+AL+ASLQSLR QFPQAVIIYADYWN YRTV+KNPS YGF+E FKACC
Sbjct: 251 VRSVSNQTYVHNVALQASLQSLRGQFPQAVIIYADYWNAYRTVIKNPSNYGFKERFKACC 310

Query: 522 GVGGPYNFNVLAVCGMSSVSSCKKPYEYINWDGVHLTEAMYKVVHDMFVEGGFTEPPFGS 581
           GVG PYNF+V  VCGMSSVSSCK P EYINWDGVHLTEAMYK+VHDMF+EGG T PPF  
Sbjct: 311 GVGEPYNFDVFTVCGMSSVSSCKNPSEYINWDGVHLTEAMYKIVHDMFIEGGLTRPPFSY 370

Query: 582 LFDKKRREG 584
           L D KRR G
Sbjct: 371 LLDMKRRLG 379

BLAST of Carg06771 vs. TAIR 10
Match: AT3G48460.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 451.1 bits (1159), Expect = 1.4e-126
Identity = 215/365 (58.90%), Postives = 271/365 (74.25%), Query Frame = 0

Query: 222 AIFILLCAFASASPTANEMHPGLFSKIYAFGDSFTDTGNTRSVSGPTVFGHVSNSPYGST 281
           ++ ILL +  S + T   +H   F+KIYAFGDSFTDTGN+RS  GP  FGH+S+ PYG T
Sbjct: 14  SVAILLFSTISTAATIPNIHRP-FNKIYAFGDSFTDTGNSRSGEGPAGFGHLSSPPYGMT 73

Query: 282 FFHHSTNRYSDGRLVIDFIAQTLSLPFLTPYRYLKGNES------FHGVNFAVAGSTAIN 341
           FF   TNRYSDGRL IDF+A++++LPFL PY  LK   +       HGVNFAV+GST I 
Sbjct: 74  FFRRPTNRYSDGRLTIDFVAESMNLPFLPPYLSLKTTNANGTATDTHGVNFAVSGSTVIK 133

Query: 342 HDFFVRNNLSLNITPQSIQTQLHWFNKFLETQG-HRGAAAFDDALFWVGEIGVNDYAYNT 401
           H FFV+NNLSL++TPQSI+T+L WF K+LET G ++  + F D+LFW+GEIGVNDYAY  
Sbjct: 134 HAFFVKNNLSLDMTPQSIETELAWFEKYLETLGTNQKVSLFKDSLFWIGEIGVNDYAYTL 193

Query: 402 GSPIPDDTIRKLGVASVTGVLQSLLKKGAKYMVVQGLPPSGCLPLAMSIAPVVDRDDIGC 461
           GS +  DTIR+L +++ T  L++LL KG KYM+VQG P +GCL LAMS+A   DRD +GC
Sbjct: 194 GSTVSSDTIRELSISTFTRFLETLLNKGVKYMLVQGHPATGCLTLAMSLAAEDDRDSLGC 253

Query: 462 VRSSNNQTYYHNLALRASLQSLRRQFPQAVIIYADYWNVYRTVMKNPSKYGFRELFKACC 521
           V+S+NNQ+Y HNLAL++ L+ LR ++P A I+YADYWN YR V+K+PSKYG  E FKACC
Sbjct: 254 VQSANNQSYTHNLALQSKLKQLRIKYPSATIVYADYWNAYRAVIKHPSKYGITEKFKACC 313

Query: 522 GVGGPYNFNVLAVCGMSSVSSCKKPYEYINWDGVHLTEAMYKVVHDMFVEGGFTEPPFGS 580
           G+G PYNF V   CG  + + CK P +YINWDGVHLTEAMYKV+ DMF++G FT P F  
Sbjct: 314 GIGEPYNFQVFQTCGTDAATVCKDPNQYINWDGVHLTEAMYKVMADMFLDGTFTRPRFSD 373

BLAST of Carg06771 vs. TAIR 10
Match: AT3G18030.1 (HAL3-like protein A )

HSP 1 Score: 320.5 bits (820), Expect = 2.9e-87
Identity = 143/186 (76.88%), Postives = 168/186 (90.32%), Query Frame = 0

Query: 10  EREVMQVNNAQRKPRILLAASGSVASIKFGNLYRSFSEWADVKAVATRAALHFIDRESLP 69
           +R+ M+VN   RKPR+LLAASGSVA+IKFGNL   F+EWA+V+AV T+++LHF+D+ SLP
Sbjct: 7   DRQDMEVNTTPRKPRVLLAASGSVAAIKFGNLCHCFTEWAEVRAVVTKSSLHFLDKLSLP 66

Query: 70  KDAVLYTDEDEWTGWNKIGDSVLHIELRRWADIMVIAPLSANTLGKIAGGLCDNLLTCVV 129
           ++  LYTDEDEW+ WNKIGD VLHIELRRWAD++VIAPLSANTLGKIAGGLCDNLLTC++
Sbjct: 67  QEVTLYTDEDEWSSWNKIGDPVLHIELRRWADVLVIAPLSANTLGKIAGGLCDNLLTCII 126

Query: 130 RAWDYNKPLFVAPAMNPYMWTNPFTERHLIAIDELGITLIPPVTKRLACGDYGNGAMAEP 189
           RAWDY KPLFVAPAMN  MW NPFTERHL+++DELGITLIPP+ KRLACGDYGNGAMAEP
Sbjct: 127 RAWDYTKPLFVAPAMNTLMWNNPFTERHLLSLDELGITLIPPIKKRLACGDYGNGAMAEP 186

Query: 190 SLIFST 196
           SLI+ST
Sbjct: 187 SLIYST 192

BLAST of Carg06771 vs. TAIR 10
Match: AT1G48605.1 (Flavoprotein )

HSP 1 Score: 313.9 bits (803), Expect = 2.7e-85
Identity = 142/182 (78.02%), Postives = 164/182 (90.11%), Query Frame = 0

Query: 14  MQVNNAQRKPRILLAASGSVASIKFGNLYRSFSEWADVKAVATRAALHFIDRESLPKDAV 73
           M+V+   RKPRILLAASGSVASIKF NL   FSEWA+VKAVA++++L+F+D+ SLP++  
Sbjct: 3   MEVDTVTRKPRILLAASGSVASIKFSNLCHCFSEWAEVKAVASKSSLNFVDKPSLPQNVT 62

Query: 74  LYTDEDEWTGWNKIGDSVLHIELRRWADIMVIAPLSANTLGKIAGGLCDNLLTCVVRAWD 133
           LYTDEDEW+ WNKIGD VLHIELRRWAD+M+IAPLSANTL KIAGGLCDNLLTC+VRAWD
Sbjct: 63  LYTDEDEWSSWNKIGDPVLHIELRRWADVMIIAPLSANTLAKIAGGLCDNLLTCIVRAWD 122

Query: 134 YNKPLFVAPAMNPYMWTNPFTERHLIAIDELGITLIPPVTKRLACGDYGNGAMAEPSLIF 193
           Y+KPLFVAPAMN  MW NPFTERHL+ +DELGITLIPP+ K+LACGDYGNGAMAEPSLI+
Sbjct: 123 YSKPLFVAPAMNTLMWNNPFTERHLVLLDELGITLIPPIKKKLACGDYGNGAMAEPSLIY 182

Query: 194 ST 196
           ST
Sbjct: 183 ST 184

BLAST of Carg06771 vs. TAIR 10
Match: AT1G28600.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 240.7 bits (613), Expect = 2.9e-63
Identity = 132/339 (38.94%), Postives = 185/339 (54.57%), Query Frame = 0

Query: 245 FSKIYAFGDSFTDTGNTRSVSGPTVFGHVSNSPYGSTFFHHSTNRYSDGRLVIDFIAQTL 304
           F  I +FGDS  DTGN   +S        +  PYG TFFHH T R  DGR+++DFIA+ +
Sbjct: 29  FKSIISFGDSIADTGNLVGLSDRNQLPVTAFPPYGETFFHHPTGRSCDGRIIMDFIAEFV 88

Query: 305 SLPFLTPYRYLKGNESFHGVNFAVAGSTAINHDFFVRNNLSLNITPQSIQTQLHWFNKFL 364
            LP++ PY   K      GVNFAVAG+TA+   F  +  +  + T  S+  QL  F K L
Sbjct: 89  GLPYVPPYFGSKNRNFDKGVNFAVAGATALKSSFLKKRGIQPH-TNVSLGVQLKSFKKSL 148

Query: 365 ETQGHRGAAAFD---DALFWVGEIGVNDYAYNTGSPIPDDTIRKL---GVASVTGVLQSL 424
                  +   D   +AL  +GEIG NDY +   +  P   + +L    +AS++  +  L
Sbjct: 149 PNLCGSPSDCRDMIGNALILMGEIGGNDYNFPFFNRKPVKEVEELVPFVIASISSTITEL 208

Query: 425 LKKGAKYMVVQGLPPSGCLPLAMSIAPVVDRDD----IGCVRSSNNQTYYHNLALRASLQ 484
           +  G K  +V G  P GC  + +++    ++D+     GC++  N    YH+  L+  L 
Sbjct: 209 IGMGGKTFLVPGEFPIGCSVVYLTLYKTSNKDEYDPSTGCLKWLNKFGEYHSEKLKVELN 268

Query: 485 SLRRQFPQAVIIYADYWNVYRTVMKNPSKYGFREL-FKACCGVGGPYNFNVLAVCGMSSV 544
            LR+ +P   IIYADY+N    + K P+K+GF E  F ACCG+GGPYNFN    CG   V
Sbjct: 269 RLRKLYPHVNIIYADYYNSLLRIFKEPAKFGFMERPFPACCGIGGPYNFNFTRKCGSVGV 328

Query: 545 SSCKKPYEYINWDGVHLTEAMYKVVHDMFVEGGFTEPPF 573
            SCK P +Y+ WDGVH+TEA YK + D  + G +  PPF
Sbjct: 329 KSCKDPSKYVGWDGVHMTEAAYKWIADGILNGPYANPPF 366

BLAST of Carg06771 vs. TAIR 10
Match: AT1G28650.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 227.6 bits (579), Expect = 2.5e-59
Identity = 131/361 (36.29%), Postives = 195/361 (54.02%), Query Frame = 0

Query: 224 FILLCAFASASPTANEMHPGLFSKIYAFGDSFTDTGNTRSVSGPTVFGHVSNSPYGSTFF 283
           F+L+  + +    ++E     +  I +FGDS  DTGN   +S        +  PYG +FF
Sbjct: 14  FLLILYYTTIVVASSESRCRRYKSIISFGDSIADTGNYVHLSNVNNLPQAAFLPYGESFF 73

Query: 284 HHSTNRYSDGRLVIDFIAQTLSLPFLTPYRYLKGNESFH-GVNFAVAGSTAINHDFFVRN 343
           H  + RYSDGRLVIDFIA+ L LP++ PY +   N SF+ G+NFAV G+TA++  F V+ 
Sbjct: 74  HPPSGRYSDGRLVIDFIAEFLGLPYVPPY-FGSQNVSFNQGINFAVYGATALDRAFLVKQ 133

Query: 344 NLSLNITPQSIQTQLHWFNKFL----ETQGHRGAAAFDDALFWVGEIGVNDYAYNTGSPI 403
            +  + T  S+  QL+ F + L     +          D+L  +GEIG NDY Y      
Sbjct: 134 GIKSDFTNISLSVQLNTFKQILPNLCASSTRDCREMLGDSLILMGEIGGNDYNYPFFEGK 193

Query: 404 PDDTIRKLG---VASVTGVLQSLLKKGAKYMVVQGLPPSGCLPLAMSI--APVVDRDDI- 463
             + I++L    + +++  +  L+  G K  +V G  P GC    +++     V+ D   
Sbjct: 194 SINEIKELVPLIIKAISSAIVDLIDLGGKTFLVPGNFPIGCSTAYLTLFQTATVEHDPFT 253

Query: 464 GCVRSSNNQTYYHNLALRASLQSLRRQFPQAVIIYADYWNVYRTVMKNPSKYGFREL-FK 523
           GC+   N    +HN  L+  L+ L++ +P   IIYADY+N    + + P+KYGF+     
Sbjct: 254 GCIPWLNKFGEHHNEQLKIELKQLQKLYPHVNIIYADYYNSLYGLFQEPAKYGFKNRPLA 313

Query: 524 ACCGVGGPYNFNVLAVCGMSSVSSCKKPYEYINWDGVHLTEAMYKVVHDMFVEGGFTEPP 573
           ACCGVGG YNF +   CG + VS C+ P EY+NWDG HLTEA Y+ +    + G +T P 
Sbjct: 314 ACCGVGGQYNFTIGKECGENGVSYCQNPSEYVNWDGYHLTEATYQKMAQGLLNGRYTTPA 373