Carg06748 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg06748
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Transcription termination factor 4
Location	Carg_Chr18: 999774 .. 1002130 (-)
RNA-Seq Expression	Carg06748
Synteny	Carg06748

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTTAGCATAAACACGGCGATGAAGATCTTCGTATTACGCAACTGAGCTCATTCCTGTCCCTCAAGTTTCGAGCTCCGGCGAGAAGAAAATGCTGCAGTTTCCGGCGTTCATGACCCAGTACCCTTGGTCCACGAGGACTATTCCGACGTCGTTTTTATTGCCGTCTCAGTGGCCCCAACCCCAAAACGAAGAGCTGCTGCTCGCCATGGAAGAGTCTGATTTTGAAGAGAAGGTTTTTGCCATTCTCTTTTGAAAAAACCCTAATATTCTTTCCTTCTATCCGATATTTTATTTGCATAACCTCCTGCTTGGACTTAATTGAGATCATCGGAATGTTGATTTTAGTTTAATTACGGCATGTTATGAATTTTTCGATGCGTGCCCGCTCTTGGTTGTGATTGTTTTGGAAAATGTTCGAGTATTTCTGATTTGTTCTTTGTTTTCTTCTGCCTAAATGGAATCCTCATCTTTTGTTTCTACGAATTAATTTTTGCCTCATTCCTAGAATTGAACTACTTGATGCACGTGCAAAGATCATATATCATGGAATTTCAAGTTCTAGAAGATCATATATCATGGAATTTCAAGTTCTAGAAGATCAGATATCATGGAATTTCAAGTTCTAGGTAGTGTAATTGATTGAAATCTGTACTTGCTCAAAGAAATTGAAATCGATACAACAAAAATCATGATAAAATTTGATCATCATGGGAAATCGTATTATAGTTTTAATAACCAGTGGGAAGAGACTGTTGTTGAATTAGATCTGTGTAGCTGTAAGGTTTTCACCTTTCCCAATTGCATCTGAAACAGTTTTCTCTTGGTCCTTTGCCTTGAATACTTGCTTTCTTTGAAGTCCTTCATCAGCACTAAGTTCATTTGTGACAGGAACCCTAGAGAAAACTTGTTGATACCCTACTTGAAGTGTTTGTTTCAGAGCACGACCCCGTTACCGCCTAGAATTAGCTCGAATATGATATTTATCCTTAGCCCTGTGACTCCAATGTGTCAGTATTTTCCATGGACGAGGCTTGTGTAACCTAAGATTACAAAGTGGTTTTTTGTCCTGGGGCCATGGCTTTGTCAGCAAGGGCTACTTCTTATATTGTATGCTAGATTACAAATCCGTTCACCAAACACAGTTGATAAACTTGAGTTTCTATTATCCATTCCTTTATTTAATTCAACATACTTCACAAATTCACTTGCCTGACTCCAAACTTCATAAAATCCAAGTTTATAAAAACTTCGAGCCACGAAGTCTTTAGCTGTACCTGTTCTTGTGGCGGTTGTGATGTCTTGTGTGTGTCATTCTGTATTTTTGTAACTACTTTCTCTCTTCCTTCTATATGAAACCCAGGATTCAAATTTTTGTCCTATTGGTCGATGATACATGCCTTGACTAGTTGAGTTTAGTTTTACCTAGATTGATGAGAGGCTTCGATTCTCACTATTTAATGAATTCAATTGGATTCCAACTTCGAGTTCGTGGTGATTCTTCTTTCGTTCAGCTACCCTCCAAAGGCCTCGAACAAAGAAGAGAAGAGGCTTCCATTTCTGTGAGTTCAACCTGGGATTCGAATCTTTGTCCTATTGATTAATGACATGTGCCTTAACTAGTTGAATTTAGTTGTGCCTAGATTGACAAGAGGTTCGATTCTCACTCTTTCATGAACTCAAATGGATTCCAACTTCAAGTTCGTGATGATTCTTCTTTCATTCAACTACCCACCAAAAGCCTCGAATGAAGAAGAGGAGAGACTTCCATTTCTTTTAGTTCAACCCAAGATTCAAATTTTAGTCTCATTGGTTGATAACATATGTCTTAACCAGTTGAATTCAAATGTGTCTAGATCAACGTTAGACTTTGATTCCTACTCTTTCATGAATTCAATTGGATTCAAACTTAGAGTTGGGAGGAACTAACAAACCATGAAATTCTCCTTGTCCACTGTTGCAATTTTGTAAGGTTTAGACCTCACTTTTGTATATTACTATCATGGCAGTGCAATGAAATCAGGAAGACAAACAGCAACCTGGTGGTGATTGGCAAAATGACTGCTGATAACGATAAGGAAGACATAGAGGCAGAGGATGACGATGCCGACAATGCTGAGGAATCTGAGGCTGAAGAATTTGAGCAGGAAACTGGTTAAAACTTACAACTACTCTGCATATTTGTGTCCTTTCTTGTCCTTGGAATCCTCAATTTGCTTGTGTATTACTAAACCAATTTTTCTTTTTTGTTCATTGTGAATCTGCCACTTGCATTCATTATAGCTTCAAATTTATGTGCCTCTTGAGCTGATGATACTTTGATCTTTGATGAATGAATATAACGTTTTCTTTTTATTTC

mRNA sequence

ATTTAGCATAAACACGGCGATGAAGATCTTCGTATTACGCAACTGAGCTCATTCCTGTCCCTCAAGTTTCGAGCTCCGGCGAGAAGAAAATGCTGCAGTTTCCGGCGTTCATGACCCAGTACCCTTGGTCCACGAGGACTATTCCGACGTCGTTTTTATTGCCGTCTCAGTGGCCCCAACCCCAAAACGAAGAGCTGCTGCTCGCCATGGAAGAGTCTGATTTTGAAGAGAAGTGCAATGAAATCAGGAAGACAAACAGCAACCTGGTGGTGATTGGCAAAATGACTGCTGATAACGATAAGGAAGACATAGAGGCAGAGGATGACGATGCCGACAATGCTGAGGAATCTGAGGCTGAAGAATTTGAGCAGGAAACTGGTTAAAACTTACAACTACTCTGCATATTTGTGTCCTTTCTTGTCCTTGGAATCCTCAATTTGCTTGTGTATTACTAAACCAATTTTTCTTTTTTGTTCATTGTGAATCTGCCACTTGCATTCATTATAGCTTCAAATTTATGTGCCTCTTGAGCTGATGATACTTTGATCTTTGATGAATGAATATAACGTTTTCTTTTTATTTC

Coding sequence (CDS)

ATGCTGCAGTTTCCGGCGTTCATGACCCAGTACCCTTGGTCCACGAGGACTATTCCGACGTCGTTTTTATTGCCGTCTCAGTGGCCCCAACCCCAAAACGAAGAGCTGCTGCTCGCCATGGAAGAGTCTGATTTTGAAGAGAAGTGCAATGAAATCAGGAAGACAAACAGCAACCTGGTGGTGATTGGCAAAATGACTGCTGATAACGATAAGGAAGACATAGAGGCAGAGGATGACGATGCCGACAATGCTGAGGAATCTGAGGCTGAAGAATTTGAGCAGGAAACTGGTTAA

Protein sequence

MLQFPAFMTQYPWSTRTIPTSFLLPSQWPQPQNEELLLAMEESDFEEKCNEIRKTNSNLVVIGKMTADNDKEDIEAEDDDADNAEESEAEEFEQETG

Homology

[Close]

BLAST of Carg06748 vs. NCBI nr
Match: XP_008438807.1 (PREDICTED: uncharacterized protein LOC103483797 [Cucumis melo] >XP_022954520.1 uncharacterized protein LOC111456762 [Cucurbita moschata] >XP_022994682.1 uncharacterized protein LOC111490324 [Cucurbita maxima] >XP_023541351.1 uncharacterized protein LOC111801554 [Cucurbita pepo subsp. pepo] >KAA0055258.1 transcription termination factor 4 [Cucumis melo var. makuwa] >KAG6573081.1 hypothetical protein SDJN03_26968, partial [Cucurbita argyrosperma subsp. sororia] >KAG7012267.1 hypothetical protein SDJN02_25019 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 186.0 bits (471), Expect = 1.5e-43
Identity = 97/97 (100.00%), Postives = 97/97 (100.00%), Query Frame = 0


Query: 1  MLQFPAFMTQYPWSTRTIPTSFLLPSQWPQPQNEELLLAMEESDFEEKCNEIRKTNSNLV 60
          MLQFPAFMTQYPWSTRTIPTSFLLPSQWPQPQNEELLLAMEESDFEEKCNEIRKTNSNLV
Sbjct: 1  MLQFPAFMTQYPWSTRTIPTSFLLPSQWPQPQNEELLLAMEESDFEEKCNEIRKTNSNLV 60

Query: 61 VIGKMTADNDKEDIEAEDDDADNAEESEAEEFEQETG 98
          VIGKMTADNDKEDIEAEDDDADNAEESEAEEFEQETG
Sbjct: 61 VIGKMTADNDKEDIEAEDDDADNAEESEAEEFEQETG 97

[Close]

BLAST of Carg06748 vs. NCBI nr
Match: XP_038894516.1 (uncharacterized protein LOC120083062 [Benincasa hispida])

HSP 1 Score: 181.4 bits (459), Expect = 3.7e-42
Identity = 95/97 (97.94%), Postives = 95/97 (97.94%), Query Frame = 0


Query: 1  MLQFPAFMTQYPWSTRTIPTSFLLPSQWPQPQNEELLLAMEESDFEEKCNEIRKTNSNLV 60
          MLQFPAFMTQYPWSTRTIPTSFLLPSQWPQPQNEELLLAMEESDFEEKCNEIRKTNSN V
Sbjct: 1  MLQFPAFMTQYPWSTRTIPTSFLLPSQWPQPQNEELLLAMEESDFEEKCNEIRKTNSNQV 60

Query: 61 VIGKMTADNDKEDIEAEDDDADNAEESEAEEFEQETG 98
          VIGKM ADNDKEDIEAEDDDADNAEESEAEEFEQETG
Sbjct: 61 VIGKMIADNDKEDIEAEDDDADNAEESEAEEFEQETG 97

[Close]

BLAST of Carg06748 vs. NCBI nr
Match: XP_004152491.1 (uncharacterized protein LOC101206898 [Cucumis sativus] >KGN64341.1 hypothetical protein Csa_014279 [Cucumis sativus])

HSP 1 Score: 181.4 bits (459), Expect = 3.7e-42
Identity = 94/97 (96.91%), Postives = 96/97 (98.97%), Query Frame = 0


Query: 1  MLQFPAFMTQYPWSTRTIPTSFLLPSQWPQPQNEELLLAMEESDFEEKCNEIRKTNSNLV 60
          MLQFPAFMTQYPWSTR IPTSFLLPSQWPQPQNEELLLAMEE+DFEEKCNEIRKTNSNLV
Sbjct: 1  MLQFPAFMTQYPWSTRKIPTSFLLPSQWPQPQNEELLLAMEEADFEEKCNEIRKTNSNLV 60

Query: 61 VIGKMTADNDKEDIEAEDDDADNAEESEAEEFEQETG 98
          VIGKMTADNDKEDIEAEDDDADNA+ESEAEEFEQETG
Sbjct: 61 VIGKMTADNDKEDIEAEDDDADNADESEAEEFEQETG 97

[Close]

BLAST of Carg06748 vs. NCBI nr
Match: XP_022137094.1 (uncharacterized protein LOC111008654 [Momordica charantia])

HSP 1 Score: 180.6 bits (457), Expect = 6.3e-42
Identity = 94/96 (97.92%), Postives = 95/96 (98.96%), Query Frame = 0


Query: 1  MLQFPAFMTQYPWSTRTIPTSFLLPSQWPQPQNEELLLAMEESDFEEKCNEIRKTNSNLV 60
          MLQFPAFMTQYPWSTRTIPTSFLLPSQWPQPQNEELLLAMEESDFEEKCNEIRKTNSNLV
Sbjct: 1  MLQFPAFMTQYPWSTRTIPTSFLLPSQWPQPQNEELLLAMEESDFEEKCNEIRKTNSNLV 60

Query: 61 VIGKMTADNDKEDIEAEDDDADNAEESEAEEFEQET 97
          VIGKM+ADNDKEDIEAEDDDADN EESEAEEFEQET
Sbjct: 61 VIGKMSADNDKEDIEAEDDDADNGEESEAEEFEQET 96

[Close]

BLAST of Carg06748 vs. NCBI nr
Match: XP_018813880.1 (uncharacterized protein LOC108985877 [Juglans regia] >KAF5476731.1 hypothetical protein F2P56_003439 [Juglans regia])

HSP 1 Score: 165.2 bits (417), Expect = 2.7e-37
Identity = 88/98 (89.80%), Postives = 91/98 (92.86%), Query Frame = 0


Query: 1  MLQFPAFMTQYPWSTRTIPTSFLLPSQWPQPQNEELLLAMEESDFEEKCNEIRKTNSNLV 60
          MLQFPAFMTQYPWSTRTIPTSFLLPSQWPQP +EELLLAMEESDFEEKCNEIRKTNSNLV
Sbjct: 1  MLQFPAFMTQYPWSTRTIPTSFLLPSQWPQPHSEELLLAMEESDFEEKCNEIRKTNSNLV 60

Query: 61 VIGKMTADNDKEDI--EAEDDDADNAEESEAEEFEQET 97
          VIGK T DNDKED   +A+DDDADNAEESE EEFEQET
Sbjct: 61 VIGKTTVDNDKEDYDNDADDDDADNAEESEGEEFEQET 98

[Close]

BLAST of Carg06748 vs. ExPASy TrEMBL
Match: A0A6J1JWJ6 (uncharacterized protein LOC111490324 OS=Cucurbita maxima OX=3661 GN=LOC111490324 PE=3 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 7.3e-44
Identity = 97/97 (100.00%), Postives = 97/97 (100.00%), Query Frame = 0


Query: 1  MLQFPAFMTQYPWSTRTIPTSFLLPSQWPQPQNEELLLAMEESDFEEKCNEIRKTNSNLV 60
          MLQFPAFMTQYPWSTRTIPTSFLLPSQWPQPQNEELLLAMEESDFEEKCNEIRKTNSNLV
Sbjct: 1  MLQFPAFMTQYPWSTRTIPTSFLLPSQWPQPQNEELLLAMEESDFEEKCNEIRKTNSNLV 60

Query: 61 VIGKMTADNDKEDIEAEDDDADNAEESEAEEFEQETG 98
          VIGKMTADNDKEDIEAEDDDADNAEESEAEEFEQETG
Sbjct: 61 VIGKMTADNDKEDIEAEDDDADNAEESEAEEFEQETG 97

[Close]

BLAST of Carg06748 vs. ExPASy TrEMBL
Match: A0A5A7UJS1 (Transcription termination factor 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold80G001000 PE=3 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 7.3e-44
Identity = 97/97 (100.00%), Postives = 97/97 (100.00%), Query Frame = 0


Query: 1  MLQFPAFMTQYPWSTRTIPTSFLLPSQWPQPQNEELLLAMEESDFEEKCNEIRKTNSNLV 60
          MLQFPAFMTQYPWSTRTIPTSFLLPSQWPQPQNEELLLAMEESDFEEKCNEIRKTNSNLV
Sbjct: 1  MLQFPAFMTQYPWSTRTIPTSFLLPSQWPQPQNEELLLAMEESDFEEKCNEIRKTNSNLV 60

Query: 61 VIGKMTADNDKEDIEAEDDDADNAEESEAEEFEQETG 98
          VIGKMTADNDKEDIEAEDDDADNAEESEAEEFEQETG
Sbjct: 61 VIGKMTADNDKEDIEAEDDDADNAEESEAEEFEQETG 97

[Close]

BLAST of Carg06748 vs. ExPASy TrEMBL
Match: A0A6J1GSM6 (uncharacterized protein LOC111456762 OS=Cucurbita moschata OX=3662 GN=LOC111456762 PE=3 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 7.3e-44
Identity = 97/97 (100.00%), Postives = 97/97 (100.00%), Query Frame = 0


Query: 1  MLQFPAFMTQYPWSTRTIPTSFLLPSQWPQPQNEELLLAMEESDFEEKCNEIRKTNSNLV 60
          MLQFPAFMTQYPWSTRTIPTSFLLPSQWPQPQNEELLLAMEESDFEEKCNEIRKTNSNLV
Sbjct: 1  MLQFPAFMTQYPWSTRTIPTSFLLPSQWPQPQNEELLLAMEESDFEEKCNEIRKTNSNLV 60

Query: 61 VIGKMTADNDKEDIEAEDDDADNAEESEAEEFEQETG 98
          VIGKMTADNDKEDIEAEDDDADNAEESEAEEFEQETG
Sbjct: 61 VIGKMTADNDKEDIEAEDDDADNAEESEAEEFEQETG 97

[Close]

BLAST of Carg06748 vs. ExPASy TrEMBL
Match: A0A1S3AXA5 (uncharacterized protein LOC103483797 OS=Cucumis melo OX=3656 GN=LOC103483797 PE=3 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 7.3e-44
Identity = 97/97 (100.00%), Postives = 97/97 (100.00%), Query Frame = 0


Query: 1  MLQFPAFMTQYPWSTRTIPTSFLLPSQWPQPQNEELLLAMEESDFEEKCNEIRKTNSNLV 60
          MLQFPAFMTQYPWSTRTIPTSFLLPSQWPQPQNEELLLAMEESDFEEKCNEIRKTNSNLV
Sbjct: 1  MLQFPAFMTQYPWSTRTIPTSFLLPSQWPQPQNEELLLAMEESDFEEKCNEIRKTNSNLV 60

Query: 61 VIGKMTADNDKEDIEAEDDDADNAEESEAEEFEQETG 98
          VIGKMTADNDKEDIEAEDDDADNAEESEAEEFEQETG
Sbjct: 61 VIGKMTADNDKEDIEAEDDDADNAEESEAEEFEQETG 97

[Close]

BLAST of Carg06748 vs. ExPASy TrEMBL
Match: A0A0A0LWF0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G046850 PE=3 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 1.8e-42
Identity = 94/97 (96.91%), Postives = 96/97 (98.97%), Query Frame = 0


Query: 1  MLQFPAFMTQYPWSTRTIPTSFLLPSQWPQPQNEELLLAMEESDFEEKCNEIRKTNSNLV 60
          MLQFPAFMTQYPWSTR IPTSFLLPSQWPQPQNEELLLAMEE+DFEEKCNEIRKTNSNLV
Sbjct: 1  MLQFPAFMTQYPWSTRKIPTSFLLPSQWPQPQNEELLLAMEEADFEEKCNEIRKTNSNLV 60

Query: 61 VIGKMTADNDKEDIEAEDDDADNAEESEAEEFEQETG 98
          VIGKMTADNDKEDIEAEDDDADNA+ESEAEEFEQETG
Sbjct: 61 VIGKMTADNDKEDIEAEDDDADNADESEAEEFEQETG 97

[Close]

BLAST of Carg06748 vs. TAIR 10
Match: AT5G63135.1 (unknown protein; Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 132.1 bits (331), Expect = 2.4e-31
Identity = 72/99 (72.73%), Postives = 83/99 (83.84%), Query Frame = 0


Query: 1  MLQFPAFMTQYPWSTRTIPTSFLLPSQWPQPQNEELLLAMEESDFEEKCNEIRKTNSNLV 60
          MLQFP  MTQ+P ST+TIP S+LLP QWPQPQNEE+LLAMEE++FEEKCNEIRK +  L 
Sbjct: 1  MLQFPTLMTQFPSSTKTIPASYLLPLQWPQPQNEEILLAMEEAEFEEKCNEIRKMSPALP 60

Query: 61 VIGKMTADNDKE--DIEAEDDDADNAEESEAEEFEQETG 98
          VIGK   +N++E  D EAEDDDADNAEES+ EEFEQETG
Sbjct: 61 VIGKPVVNNEQEEDDNEAEDDDADNAEESDGEEFEQETG 99

The following BLAST results are available for this feature:

BLAST of Carg06748 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008438807.1	1.5e-43	100.00	PREDICTED: uncharacterized protein LOC103483797 [Cucumis melo] >XP_022954520.1 u...	[more]
XP_038894516.1	3.7e-42	97.94	uncharacterized protein LOC120083062 [Benincasa hispida]	[more]
XP_004152491.1	3.7e-42	96.91	uncharacterized protein LOC101206898 [Cucumis sativus] >KGN64341.1 hypothetical ...	[more]
XP_022137094.1	6.3e-42	97.92	uncharacterized protein LOC111008654 [Momordica charantia]	[more]
XP_018813880.1	2.7e-37	89.80	uncharacterized protein LOC108985877 [Juglans regia] >KAF5476731.1 hypothetical ...	[more]

BLAST of Carg06748 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Carg06748 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JWJ6	7.3e-44	100.00	uncharacterized protein LOC111490324 OS=Cucurbita maxima OX=3661 GN=LOC111490324...	[more]
A0A5A7UJS1	7.3e-44	100.00	Transcription termination factor 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C...	[more]
A0A6J1GSM6	7.3e-44	100.00	uncharacterized protein LOC111456762 OS=Cucurbita moschata OX=3662 GN=LOC1114567...	[more]
A0A1S3AXA5	7.3e-44	100.00	uncharacterized protein LOC103483797 OS=Cucumis melo OX=3656 GN=LOC103483797 PE=...	[more]
A0A0A0LWF0	1.8e-42	96.91	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G046850 PE=3 SV=1	[more]

BLAST of Carg06748 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G63135.1	2.4e-31	72.73	unknown protein; Has 30201 Blast hits to 17322 proteins in 780 species: Archae -...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 67..87
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 68..97
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 72..97
None	No IPR available	PANTHER	PTHR37771	OS02G0593400 PROTEIN	coord: 1..97
None	No IPR available	PANTHER	PTHR37771:SF2	OS02G0593400 PROTEIN	coord: 1..97
IPR026182	Anaphase-promoting complex subunit 15	PFAM	PF15243	ANAPC15	coord: 9..95 e-value: 1.3E-18 score: 67.1

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg06748-RA	Carg06748-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090266	regulation of mitotic cell cycle spindle assembly checkpoint
cellular_component	GO:0005680	anaphase-promoting complex