Carg06694 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg06694
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Dynein light chain, cytoplasmic
Location	Carg_Chr18: 701935 .. 702978 (+)
RNA-Seq Expression	Carg06694
Synteny	Carg06694

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGAAACCCACCAGCCATCTCCCCCGTTTAGCCCCTCCTGATCCTGATTCAGTGGCTAATCCCCCCACATGCCCGAAGCTTGAATCGGCCATGGCTGCCGCTGCTGCTCCTAAGCCCTCTTCCTTGCCCAAACTCCATAATCAGCCCCTTGTGGCTGTTTCTTCTATGATCAAATCAAAACCCCACCTCCAGAAATTTGCTAATGAAGAGAAGGAATTGAAAGGGCTTCAAAAGGGGCATCGCCCTGACAGTATGACACCAACAGTGTCCTTACTTCGAAAGGGTGGAAGAACAAAGTCCTTTGCAGCCTCCCAAGTTGAATTGGCTGATATCTTTGCAAAGAACGGCATCAAAGTGGTATCAGTTGACATGCCACCGGCTATGCAGATTCATGCAGTGGATTATGCAAGAAAAGCTCATGACAGTATGGAGAAATTCACTTCTAAAGCTCTTGCTCTGTCTCTCAAGAGGGTAATTAGCTTAAGGTTCATGTGCAATGTTCAAGTTCTTGAATGATCAGCCAATAACTTTTATGGGTTTGTTTGTGATTCTTTCAGGAGTTTGATGGGGCTTATGGCCCGGTCTGGCACTGCATCGTGGGGACAAGTTTTGGGTCCTTTGTGACTCATTCAATTGGTGGGTTCTTATATCTATCAATGGACCAAAAGCTGTATGTTCTATTGTTCAAAACCTCTGTACAAAGAGCTGATTGAAGTAGATGGCCAAGGTAAAAGAGACTCTTCATCCTAATACTTGTACATTTGTTCTCATAAGAAACACACTAGCCATACAGTATATTTCTCCTATAATATGTTTAGGGATGTCTTATGAGCTGTTGATAGAGAAATTTTCATTGCAATTATTGAGGTTTAGTGATGGAATAACCCTTTCTTTACGACTCAAATACCGTGTTTGGATTGTATACACGTTATCTTTGAGATCCCATGTAGGTTGGAGAGGGAAACGAAGTATTCTTTATAAGGGTGTGGAAACCTCTCCCTAGTAGATGTGTTTTAGAATTGTGAGGCTGATGGCGATATG

mRNA sequence

ATGGCGAAACCCACCAGCCATCTCCCCCGTTTAGCCCCTCCTGATCCTGATTCAGTGGCTAATCCCCCCACATGCCCGAAGCTTGAATCGGCCATGGCTGCCGCTGCTGCTCCTAAGCCCTCTTCCTTGCCCAAACTCCATAATCAGCCCCTTGTGGCTGTTTCTTCTATGATCAAATCAAAACCCCACCTCCAGAAATTTGCTAATGAAGAGAAGGAATTGAAAGGGCTTCAAAAGGGGCATCGCCCTGACAGTATGACACCAACAGTGTCCTTACTTCGAAAGGGTGGAAGAACAAAGTCCTTTGCAGCCTCCCAAGTTGAATTGGCTGATATCTTTGCAAAGAACGGCATCAAAGTGGTATCAGTTGACATGCCACCGGCTATGCAGATTCATGCAGTGGATTATGCAAGAAAAGCTCATGACAGTATGGAGAAATTCACTTCTAAAGCTCTTGCTCTGTCTCTCAAGAGGGAGTTTGATGGGGCTTATGGCCCGGTCTGGCACTGCATCGTGGGGACAAGTTTTGGGTCCTTTGTGACTCATTCAATTGGTGGGTTCTTATATCTATCAATGGACCAAAAGCTGTATGTTCTATTGTTCAAAACCTCTGTACAAAGAGCTGATTGAAGTAGATGGCCAAGGTAAAAGAGACTCTTCATCCTAATACTTGTACATTTGTTCTCATAAGAAACACACTAGCCATACAGTATATTTCTCCTATAATATGTTTAGGGATGTCTTATGAGCTGTTGATAGAGAAATTTTCATTGCAATTATTGAGGTTTAGTGATGGAATAACCCTTTCTTTACGACTCAAATACCGTGTTTGGATTGTATACACGTTATCTTTGAGATCCCATGTAGGTTGGAGAGGGAAACGAAGTATTCTTTATAAGGGTGTGGAAACCTCTCCCTAGTAGATGTGTTTTAGAATTGTGAGGCTGATGGCGATATG

Coding sequence (CDS)

ATGGCGAAACCCACCAGCCATCTCCCCCGTTTAGCCCCTCCTGATCCTGATTCAGTGGCTAATCCCCCCACATGCCCGAAGCTTGAATCGGCCATGGCTGCCGCTGCTGCTCCTAAGCCCTCTTCCTTGCCCAAACTCCATAATCAGCCCCTTGTGGCTGTTTCTTCTATGATCAAATCAAAACCCCACCTCCAGAAATTTGCTAATGAAGAGAAGGAATTGAAAGGGCTTCAAAAGGGGCATCGCCCTGACAGTATGACACCAACAGTGTCCTTACTTCGAAAGGGTGGAAGAACAAAGTCCTTTGCAGCCTCCCAAGTTGAATTGGCTGATATCTTTGCAAAGAACGGCATCAAAGTGGTATCAGTTGACATGCCACCGGCTATGCAGATTCATGCAGTGGATTATGCAAGAAAAGCTCATGACAGTATGGAGAAATTCACTTCTAAAGCTCTTGCTCTGTCTCTCAAGAGGGAGTTTGATGGGGCTTATGGCCCGGTCTGGCACTGCATCGTGGGGACAAGTTTTGGGTCCTTTGTGACTCATTCAATTGGTGGGTTCTTATATCTATCAATGGACCAAAAGCTGTATGTTCTATTGTTCAAAACCTCTGTACAAAGAGCTGATTGA

Protein sequence

MAKPTSHLPRLAPPDPDSVANPPTCPKLESAMAAAAAPKPSSLPKLHNQPLVAVSSMIKSKPHLQKFANEEKELKGLQKGHRPDSMTPTVSLLRKGGRTKSFAASQVELADIFAKNGIKVVSVDMPPAMQIHAVDYARKAHDSMEKFTSKALALSLKREFDGAYGPVWHCIVGTSFGSFVTHSIGGFLYLSMDQKLYVLLFKTSVQRAD

Homology

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BLAST of Carg06694 vs. NCBI nr
Match: KAG6573027.1 (hypothetical protein SDJN03_26914, partial [Cucurbita argyrosperma subsp. sororia] >KAG7012215.1 hypothetical protein SDJN02_24967, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 409.8 bits (1052), Expect = 1.4e-110
Identity = 209/209 (100.00%), Postives = 209/209 (100.00%), Query Frame = 0


Query: 1   MAKPTSHLPRLAPPDPDSVANPPTCPKLESAMAAAAAPKPSSLPKLHNQPLVAVSSMIKS 60
           MAKPTSHLPRLAPPDPDSVANPPTCPKLESAMAAAAAPKPSSLPKLHNQPLVAVSSMIKS
Sbjct: 1   MAKPTSHLPRLAPPDPDSVANPPTCPKLESAMAAAAAPKPSSLPKLHNQPLVAVSSMIKS 60

Query: 61  KPHLQKFANEEKELKGLQKGHRPDSMTPTVSLLRKGGRTKSFAASQVELADIFAKNGIKV 120
           KPHLQKFANEEKELKGLQKGHRPDSMTPTVSLLRKGGRTKSFAASQVELADIFAKNGIKV
Sbjct: 61  KPHLQKFANEEKELKGLQKGHRPDSMTPTVSLLRKGGRTKSFAASQVELADIFAKNGIKV 120

Query: 121 VSVDMPPAMQIHAVDYARKAHDSMEKFTSKALALSLKREFDGAYGPVWHCIVGTSFGSFV 180
           VSVDMPPAMQIHAVDYARKAHDSMEKFTSKALALSLKREFDGAYGPVWHCIVGTSFGSFV
Sbjct: 121 VSVDMPPAMQIHAVDYARKAHDSMEKFTSKALALSLKREFDGAYGPVWHCIVGTSFGSFV 180

Query: 181 THSIGGFLYLSMDQKLYVLLFKTSVQRAD 210
           THSIGGFLYLSMDQKLYVLLFKTSVQRAD
Sbjct: 181 THSIGGFLYLSMDQKLYVLLFKTSVQRAD 209

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BLAST of Carg06694 vs. NCBI nr
Match: XP_022954796.1 (uncharacterized protein LOC111456948 [Cucurbita moschata] >XP_022954797.1 uncharacterized protein LOC111456948 [Cucurbita moschata])

HSP 1 Score: 402.1 bits (1032), Expect = 2.9e-108
Identity = 205/209 (98.09%), Postives = 207/209 (99.04%), Query Frame = 0


Query: 1   MAKPTSHLPRLAPPDPDSVANPPTCPKLESAMAAAAAPKPSSLPKLHNQPLVAVSSMIKS 60
           MAKPTSHLPRLAPPDPDSVANPPTCPKLESAMAAAAAPKPSSLPKLHNQPLVAVSSMIKS
Sbjct: 1   MAKPTSHLPRLAPPDPDSVANPPTCPKLESAMAAAAAPKPSSLPKLHNQPLVAVSSMIKS 60

Query: 61  KPHLQKFANEEKELKGLQKGHRPDSMTPTVSLLRKGGRTKSFAASQVELADIFAKNGIKV 120
           KPHLQKFANE+KELKGLQKGH PDSM PTVSLLRKGGRTKSFAASQVELADIFAKNGIKV
Sbjct: 61  KPHLQKFANEKKELKGLQKGHHPDSMRPTVSLLRKGGRTKSFAASQVELADIFAKNGIKV 120

Query: 121 VSVDMPPAMQIHAVDYARKAHDSMEKFTSKALALSLKREFDGAYGPVWHCIVGTSFGSFV 180
           VSVDMPPAMQIHAV+YARKAHDSMEKFTSKALALSLKREFDGAYGPVWHCIVGTSFGSFV
Sbjct: 121 VSVDMPPAMQIHAVNYARKAHDSMEKFTSKALALSLKREFDGAYGPVWHCIVGTSFGSFV 180

Query: 181 THSIGGFLYLSMDQKLYVLLFKTSVQRAD 210
           THSIGGFLYLSMDQKLYVLLFKTSVQRAD
Sbjct: 181 THSIGGFLYLSMDQKLYVLLFKTSVQRAD 209

[Close]

BLAST of Carg06694 vs. NCBI nr
Match: XP_022994662.1 (uncharacterized protein LOC111490306 isoform X2 [Cucurbita maxima])

HSP 1 Score: 379.0 bits (972), Expect = 2.6e-101
Identity = 194/209 (92.82%), Postives = 198/209 (94.74%), Query Frame = 0


Query: 1   MAKPTSHLPRLAPPDPDSVANPPTCPKLESAMAAAAAPKPSSLPKLHNQPLVAVSSMIKS 60
           MAKP+SHLP LAPPDPDSVANPPTCPKLESAMAAAAAPKPS   KLHNQPLVAVSSMI S
Sbjct: 27  MAKPSSHLPSLAPPDPDSVANPPTCPKLESAMAAAAAPKPSPFTKLHNQPLVAVSSMINS 86

Query: 61  KPHLQKFANEEKELKGLQKGHRPDSMTPTVSLLRKGGRTKSFAASQVELADIFAKNGIKV 120
           KP LQKF NEEKELKG+QKGH PDSM PTVSLLRKGGRTKSFAASQVEL DIFAKNG+KV
Sbjct: 87  KPRLQKFDNEEKELKGIQKGHCPDSMRPTVSLLRKGGRTKSFAASQVELIDIFAKNGMKV 146

Query: 121 VSVDMPPAMQIHAVDYARKAHDSMEKFTSKALALSLKREFDGAYGPVWHCIVGTSFGSFV 180
           VSVDMPPAMQIHAVD ARKAHDSMEKFTSKALALSLKREFDGAYGPVWHCIVGTSFGSFV
Sbjct: 147 VSVDMPPAMQIHAVDCARKAHDSMEKFTSKALALSLKREFDGAYGPVWHCIVGTSFGSFV 206

Query: 181 THSIGGFLYLSMDQKLYVLLFKTSVQRAD 210
           THS+GGFLYLSMDQKLYVLLFKTSVQRAD
Sbjct: 207 THSVGGFLYLSMDQKLYVLLFKTSVQRAD 235

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BLAST of Carg06694 vs. NCBI nr
Match: XP_023541664.1 (uncharacterized protein LOC111801755 [Cucurbita pepo subsp. pepo] >XP_023541665.1 uncharacterized protein LOC111801755 [Cucurbita pepo subsp. pepo] >XP_023541666.1 uncharacterized protein LOC111801755 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 373.2 bits (957), Expect = 1.4e-99
Identity = 192/209 (91.87%), Postives = 199/209 (95.22%), Query Frame = 0


Query: 1   MAKPTSHLPRLAPPDPDSVANPPTCPKLESAMAAAAAPKPSSLPKLHNQPLVAVSSMIKS 60
           MAKP+SHLP LAPPDPDSVANPPTCPKLESAMAAAAAPK +     +  PL+AVSSMIKS
Sbjct: 1   MAKPSSHLPSLAPPDPDSVANPPTCPKLESAMAAAAAPKSA-----NPSPLMAVSSMIKS 60

Query: 61  KPHLQKFANEEKELKGLQKGHRPDSMTPTVSLLRKGGRTKSFAASQVELADIFAKNGIKV 120
           KPHLQKFANEEKELKGLQKGHRPDSM PTVSLLRKGGRTKSFAASQVELADIFAKNG+KV
Sbjct: 61  KPHLQKFANEEKELKGLQKGHRPDSMRPTVSLLRKGGRTKSFAASQVELADIFAKNGMKV 120

Query: 121 VSVDMPPAMQIHAVDYARKAHDSMEKFTSKALALSLKREFDGAYGPVWHCIVGTSFGSFV 180
           VSVDMPPAMQIHAVDYARKAHDSMEKFTSKALALSLKREFDGAYGPVWHCIVGTSFGSFV
Sbjct: 121 VSVDMPPAMQIHAVDYARKAHDSMEKFTSKALALSLKREFDGAYGPVWHCIVGTSFGSFV 180

Query: 181 THSIGGFLYLSMDQKLYVLLFKTSVQRAD 210
           THS+GGFLYL+MDQKLYVLLFKTSVQRAD
Sbjct: 181 THSVGGFLYLAMDQKLYVLLFKTSVQRAD 204

[Close]

BLAST of Carg06694 vs. NCBI nr
Match: XP_022994659.1 (uncharacterized protein LOC111490306 isoform X1 [Cucurbita maxima] >XP_022994660.1 uncharacterized protein LOC111490306 isoform X1 [Cucurbita maxima] >XP_022994661.1 uncharacterized protein LOC111490306 isoform X1 [Cucurbita maxima])

HSP 1 Score: 369.4 bits (947), Expect = 2.1e-98
Identity = 194/223 (87.00%), Postives = 198/223 (88.79%), Query Frame = 0


Query: 1   MAKPTSHLPRLAPPDPDSVANPPTCPKLESAMAAAAAPKPSSLPKLHNQPLV-------- 60
           MAKP+SHLP LAPPDPDSVANPPTCPKLESAMAAAAAPKPS   KLHNQPLV        
Sbjct: 27  MAKPSSHLPSLAPPDPDSVANPPTCPKLESAMAAAAAPKPSPFTKLHNQPLVSSQRLKSS 86

Query: 61  ------AVSSMIKSKPHLQKFANEEKELKGLQKGHRPDSMTPTVSLLRKGGRTKSFAASQ 120
                 AVSSMI SKP LQKF NEEKELKG+QKGH PDSM PTVSLLRKGGRTKSFAASQ
Sbjct: 87  NPSPRMAVSSMINSKPRLQKFDNEEKELKGIQKGHCPDSMRPTVSLLRKGGRTKSFAASQ 146

Query: 121 VELADIFAKNGIKVVSVDMPPAMQIHAVDYARKAHDSMEKFTSKALALSLKREFDGAYGP 180
           VEL DIFAKNG+KVVSVDMPPAMQIHAVD ARKAHDSMEKFTSKALALSLKREFDGAYGP
Sbjct: 147 VELIDIFAKNGMKVVSVDMPPAMQIHAVDCARKAHDSMEKFTSKALALSLKREFDGAYGP 206

Query: 181 VWHCIVGTSFGSFVTHSIGGFLYLSMDQKLYVLLFKTSVQRAD 210
           VWHCIVGTSFGSFVTHS+GGFLYLSMDQKLYVLLFKTSVQRAD
Sbjct: 207 VWHCIVGTSFGSFVTHSVGGFLYLSMDQKLYVLLFKTSVQRAD 249

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BLAST of Carg06694 vs. ExPASy Swiss-Prot
Match: O02414 (Dynein light chain LC6, flagellar outer arm OS=Heliocidaris crassispina OX=1043166 PE=3 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 3.1e-12
Identity = 41/85 (48.24%), Postives = 54/85 (63.53%), Query Frame = 0


Query: 120 VVSVDMPPAMQIHAVDYARKAHDSMEKFT-SKALALSLKREFDGAYGPVWHCIVGTSFGS 179
           + + DMP  MQ  AVD A +A   +EKF   K +A  +K+EFD  Y P WHCIVG +FGS
Sbjct: 8   IKNADMPEDMQQDAVDCATQA---LEKFNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGS 67

Query: 180 FVTHSIGGFLYLSMDQKLYVLLFKT 204
           +VTH    F+Y  + Q + VLLFK+
Sbjct: 68  YVTHETKHFIYFYLGQ-VAVLLFKS 88

[Close]

BLAST of Carg06694 vs. ExPASy Swiss-Prot
Match: Q86A88 (Dynein light chain, cytoplasmic OS=Dictyostelium discoideum OX=44689 GN=dlcB PE=3 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 6.8e-12
Identity = 40/85 (47.06%), Postives = 52/85 (61.18%), Query Frame = 0


Query: 118 IKVVSVDMPPAMQIHAVDYARKAHDSMEKFTSKALALSLKREFDGAYGPVWHCIVGTSFG 177
           I V + DMP  MQ  A +   KA +  E    + +A+ +K+EFD  Y P WHCIVG SFG
Sbjct: 8   INVKNADMPDFMQQDATECTIKAFE--ETNIERDIAMIIKKEFDKKYSPTWHCIVGKSFG 67

Query: 178 SFVTHSIGGFLYLSMDQKLYVLLFK 203
           SFVTH    F+Y +++ K  VLLFK
Sbjct: 68  SFVTHETKNFIYFNIN-KHSVLLFK 89

[Close]

BLAST of Carg06694 vs. ExPASy Swiss-Prot
Match: Q3MHR3 (Dynein light chain 2, cytoplasmic OS=Bos taurus OX=9913 GN=DYNLL2 PE=3 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 3.4e-11
Identity = 39/85 (45.88%), Postives = 53/85 (62.35%), Query Frame = 0


Query: 120 VVSVDMPPAMQIHAVDYARKAHDSMEKFT-SKALALSLKREFDGAYGPVWHCIVGTSFGS 179
           + + DM   MQ  AVD A +A   MEK+   K +A  +K+EFD  Y P WHCIVG +FGS
Sbjct: 8   IKNADMSEDMQQDAVDCATQA---MEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGS 67

Query: 180 FVTHSIGGFLYLSMDQKLYVLLFKT 204
           +VTH    F+Y  + Q + +LLFK+
Sbjct: 68  YVTHETKHFIYFYLGQ-VAILLFKS 88

[Close]

BLAST of Carg06694 vs. ExPASy Swiss-Prot
Match: Q96FJ2 (Dynein light chain 2, cytoplasmic OS=Homo sapiens OX=9606 GN=DYNLL2 PE=1 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 3.4e-11
Identity = 39/85 (45.88%), Postives = 53/85 (62.35%), Query Frame = 0


Query: 120 VVSVDMPPAMQIHAVDYARKAHDSMEKFT-SKALALSLKREFDGAYGPVWHCIVGTSFGS 179
           + + DM   MQ  AVD A +A   MEK+   K +A  +K+EFD  Y P WHCIVG +FGS
Sbjct: 8   IKNADMSEDMQQDAVDCATQA---MEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGS 67

Query: 180 FVTHSIGGFLYLSMDQKLYVLLFKT 204
           +VTH    F+Y  + Q + +LLFK+
Sbjct: 68  YVTHETKHFIYFYLGQ-VAILLFKS 88

[Close]

BLAST of Carg06694 vs. ExPASy Swiss-Prot
Match: Q9D0M5 (Dynein light chain 2, cytoplasmic OS=Mus musculus OX=10090 GN=Dynll2 PE=1 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 3.4e-11
Identity = 39/85 (45.88%), Postives = 53/85 (62.35%), Query Frame = 0


Query: 120 VVSVDMPPAMQIHAVDYARKAHDSMEKFT-SKALALSLKREFDGAYGPVWHCIVGTSFGS 179
           + + DM   MQ  AVD A +A   MEK+   K +A  +K+EFD  Y P WHCIVG +FGS
Sbjct: 8   IKNADMSEDMQQDAVDCATQA---MEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGS 67

Query: 180 FVTHSIGGFLYLSMDQKLYVLLFKT 204
           +VTH    F+Y  + Q + +LLFK+
Sbjct: 68  YVTHETKHFIYFYLGQ-VAILLFKS 88

[Close]

BLAST of Carg06694 vs. ExPASy TrEMBL
Match: A0A6J1GRW3 (uncharacterized protein LOC111456948 OS=Cucurbita moschata OX=3662 GN=LOC111456948 PE=4 SV=1)

HSP 1 Score: 402.1 bits (1032), Expect = 1.4e-108
Identity = 205/209 (98.09%), Postives = 207/209 (99.04%), Query Frame = 0


Query: 1   MAKPTSHLPRLAPPDPDSVANPPTCPKLESAMAAAAAPKPSSLPKLHNQPLVAVSSMIKS 60
           MAKPTSHLPRLAPPDPDSVANPPTCPKLESAMAAAAAPKPSSLPKLHNQPLVAVSSMIKS
Sbjct: 1   MAKPTSHLPRLAPPDPDSVANPPTCPKLESAMAAAAAPKPSSLPKLHNQPLVAVSSMIKS 60

Query: 61  KPHLQKFANEEKELKGLQKGHRPDSMTPTVSLLRKGGRTKSFAASQVELADIFAKNGIKV 120
           KPHLQKFANE+KELKGLQKGH PDSM PTVSLLRKGGRTKSFAASQVELADIFAKNGIKV
Sbjct: 61  KPHLQKFANEKKELKGLQKGHHPDSMRPTVSLLRKGGRTKSFAASQVELADIFAKNGIKV 120

Query: 121 VSVDMPPAMQIHAVDYARKAHDSMEKFTSKALALSLKREFDGAYGPVWHCIVGTSFGSFV 180
           VSVDMPPAMQIHAV+YARKAHDSMEKFTSKALALSLKREFDGAYGPVWHCIVGTSFGSFV
Sbjct: 121 VSVDMPPAMQIHAVNYARKAHDSMEKFTSKALALSLKREFDGAYGPVWHCIVGTSFGSFV 180

Query: 181 THSIGGFLYLSMDQKLYVLLFKTSVQRAD 210
           THSIGGFLYLSMDQKLYVLLFKTSVQRAD
Sbjct: 181 THSIGGFLYLSMDQKLYVLLFKTSVQRAD 209

[Close]

BLAST of Carg06694 vs. ExPASy TrEMBL
Match: A0A6J1JWH6 (uncharacterized protein LOC111490306 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111490306 PE=4 SV=1)

HSP 1 Score: 379.0 bits (972), Expect = 1.3e-101
Identity = 194/209 (92.82%), Postives = 198/209 (94.74%), Query Frame = 0


Query: 1   MAKPTSHLPRLAPPDPDSVANPPTCPKLESAMAAAAAPKPSSLPKLHNQPLVAVSSMIKS 60
           MAKP+SHLP LAPPDPDSVANPPTCPKLESAMAAAAAPKPS   KLHNQPLVAVSSMI S
Sbjct: 27  MAKPSSHLPSLAPPDPDSVANPPTCPKLESAMAAAAAPKPSPFTKLHNQPLVAVSSMINS 86

Query: 61  KPHLQKFANEEKELKGLQKGHRPDSMTPTVSLLRKGGRTKSFAASQVELADIFAKNGIKV 120
           KP LQKF NEEKELKG+QKGH PDSM PTVSLLRKGGRTKSFAASQVEL DIFAKNG+KV
Sbjct: 87  KPRLQKFDNEEKELKGIQKGHCPDSMRPTVSLLRKGGRTKSFAASQVELIDIFAKNGMKV 146

Query: 121 VSVDMPPAMQIHAVDYARKAHDSMEKFTSKALALSLKREFDGAYGPVWHCIVGTSFGSFV 180
           VSVDMPPAMQIHAVD ARKAHDSMEKFTSKALALSLKREFDGAYGPVWHCIVGTSFGSFV
Sbjct: 147 VSVDMPPAMQIHAVDCARKAHDSMEKFTSKALALSLKREFDGAYGPVWHCIVGTSFGSFV 206

Query: 181 THSIGGFLYLSMDQKLYVLLFKTSVQRAD 210
           THS+GGFLYLSMDQKLYVLLFKTSVQRAD
Sbjct: 207 THSVGGFLYLSMDQKLYVLLFKTSVQRAD 235

[Close]

BLAST of Carg06694 vs. ExPASy TrEMBL
Match: A0A6J1K3H0 (uncharacterized protein LOC111490306 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111490306 PE=4 SV=1)

HSP 1 Score: 369.4 bits (947), Expect = 1.0e-98
Identity = 194/223 (87.00%), Postives = 198/223 (88.79%), Query Frame = 0


Query: 1   MAKPTSHLPRLAPPDPDSVANPPTCPKLESAMAAAAAPKPSSLPKLHNQPLV-------- 60
           MAKP+SHLP LAPPDPDSVANPPTCPKLESAMAAAAAPKPS   KLHNQPLV        
Sbjct: 27  MAKPSSHLPSLAPPDPDSVANPPTCPKLESAMAAAAAPKPSPFTKLHNQPLVSSQRLKSS 86

Query: 61  ------AVSSMIKSKPHLQKFANEEKELKGLQKGHRPDSMTPTVSLLRKGGRTKSFAASQ 120
                 AVSSMI SKP LQKF NEEKELKG+QKGH PDSM PTVSLLRKGGRTKSFAASQ
Sbjct: 87  NPSPRMAVSSMINSKPRLQKFDNEEKELKGIQKGHCPDSMRPTVSLLRKGGRTKSFAASQ 146

Query: 121 VELADIFAKNGIKVVSVDMPPAMQIHAVDYARKAHDSMEKFTSKALALSLKREFDGAYGP 180
           VEL DIFAKNG+KVVSVDMPPAMQIHAVD ARKAHDSMEKFTSKALALSLKREFDGAYGP
Sbjct: 147 VELIDIFAKNGMKVVSVDMPPAMQIHAVDCARKAHDSMEKFTSKALALSLKREFDGAYGP 206

Query: 181 VWHCIVGTSFGSFVTHSIGGFLYLSMDQKLYVLLFKTSVQRAD 210
           VWHCIVGTSFGSFVTHS+GGFLYLSMDQKLYVLLFKTSVQRAD
Sbjct: 207 VWHCIVGTSFGSFVTHSVGGFLYLSMDQKLYVLLFKTSVQRAD 249

[Close]

BLAST of Carg06694 vs. ExPASy TrEMBL
Match: A0A6J1E7P6 (uncharacterized protein LOC111431561 OS=Cucurbita moschata OX=3662 GN=LOC111431561 PE=4 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 9.4e-65
Identity = 159/288 (55.21%), Postives = 176/288 (61.11%), Query Frame = 0


Query: 1   MAKPTSHLPRLAPPDPD--------------SVANPPTCP-KLESAMAAAAAPKPSSLP- 60
           MAKPT+ L  LAPPDPD              S+AN  + P KL+SA+AAAA P P+  P 
Sbjct: 1   MAKPTNQLRILAPPDPDSPPLKPPFRAPPSASMANNHSTPAKLDSAIAAAAPPPPNPSPL 60

Query: 61  -------KLHNQ--------------PLVAVSSMIKSKPHLQKFAN-------------- 120
                  KLH Q              P + VS+M+KSKPHLQK A+              
Sbjct: 61  SNHIPFSKLHRQSDFSSQKPKNPNPPPPLTVSTMVKSKPHLQKIASGGDEKLSKLCKEMG 120

Query: 121 ----------------------------EEKELKGLQKGHRPDSMTPTVSLLRKGGRTKS 180
                                       +EKELK  QKGH   ++ PTVSLLRK GR +S
Sbjct: 121 TKKFFDEKKEANKGVNDDSKALYLVVKEKEKELKEPQKGHGLHTLRPTVSLLRKDGRRRS 180

Query: 181 FAASQVELADIFAKNGIKVVSVDMPPAMQIHAVDYARKAHDSMEKFTSKALALSLKREFD 210
            A  QVELADI AKNG+KVVSVDMPPAMQIHAVD ARKAHDSMEKFTSK LALSLKREFD
Sbjct: 181 LADPQVELADILAKNGVKVVSVDMPPAMQIHAVDCARKAHDSMEKFTSKTLALSLKREFD 240

[Close]

BLAST of Carg06694 vs. ExPASy TrEMBL
Match: A0A6J1KET7 (uncharacterized protein LOC111495174 OS=Cucurbita maxima OX=3661 GN=LOC111495174 PE=4 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 8.8e-63
Identity = 157/287 (54.70%), Postives = 176/287 (61.32%), Query Frame = 0


Query: 1   MAKPTSHLPRLAPPDPD--------------SVANPPTCP-KLESAMAAAAA-PKPSSL- 60
           MAKPT+ L  LAPPDPD              S+AN  + P KL+SA+AAA++ P PS L 
Sbjct: 1   MAKPTNQLRILAPPDPDSPPLKPPFRAPPSASMANNHSTPAKLDSAIAAASSPPNPSPLS 60

Query: 61  -----PKLHNQ--------------PLVAVSSMIKSKPHLQKFAN--------------- 120
                 +LH Q              P + V +M+KSKPHLQK A+               
Sbjct: 61  NHIPFSELHRQPGCLSQKPKNSNPPPSLTVPTMVKSKPHLQKIASGGDEKLSKLCKEMGT 120

Query: 121 ---------------------------EEKELKGLQKGHRPDSMTPTVSLLRKGGRTKSF 180
                                      +EKELK  QKGH   ++ PTVSLLRK GR +S 
Sbjct: 121 KKFFDEKKEANKGVDDDSKALYLVVKEKEKELKEPQKGHGLHTLRPTVSLLRKDGRRRSL 180

Query: 181 AASQVELADIFAKNGIKVVSVDMPPAMQIHAVDYARKAHDSMEKFTSKALALSLKREFDG 210
           A  QVELADI AKNG+KVVSVDMPPAMQIHAVD ARKAHDSMEKFTSK LALSLKREFD 
Sbjct: 181 ADPQVELADILAKNGVKVVSVDMPPAMQIHAVDCARKAHDSMEKFTSKTLALSLKREFDS 240

[Close]

BLAST of Carg06694 vs. TAIR 10
Match: AT5G20110.1 (Dynein light chain type 1 family protein )

HSP 1 Score: 117.9 bits (294), Expect = 1.0e-26
Identity = 57/91 (62.64%), Postives = 70/91 (76.92%), Query Frame = 0


Query: 118 IKVVSVDMPPAMQIHAVDYARKAHDSMEKFTSKALALSLKREFDGAYGPVWHCIVGTSFG 177
           +++++ DMP  MQ HA   AR   DS+EKF+SK +A +LK+EFD  YGP WHCIVG+SFG
Sbjct: 117 VRILAADMPGFMQAHAFRCARMTLDSLEKFSSKHMAFNLKKEFDKGYGPAWHCIVGSSFG 176

Query: 178 SFVTHSIGGFLYLSMDQKLYVLLFKTSVQRA 209
           SFVTHS G F+Y SMD KLYVLLFKT V+ A
Sbjct: 177 SFVTHSTGCFIYFSMD-KLYVLLFKTKVRPA 206

[Close]

BLAST of Carg06694 vs. TAIR 10
Match: AT1G23220.1 (Dynein light chain type 1 family protein )

HSP 1 Score: 98.2 bits (243), Expect = 8.2e-21
Identity = 55/111 (49.55%), Postives = 72/111 (64.86%), Query Frame = 0


Query: 100 KSFAASQVELADIFAKNGIKVVSVDMPPAMQIHAVDYARKAHDSME-KFTSKALALSLKR 159
           K  A  Q +  D F    ++V + DMP   Q  A   +R+  ++   K  +K LA +LK+
Sbjct: 21  KKKAKEQQDQKDEF---NVRVRASDMPLPQQNRAFSLSREILNATPGKADNKRLAHALKK 80

Query: 160 EFDGAYGPVWHCIVGTSFGSFVTHSIGGFLYLSMDQKLYVLLFKTSVQRAD 210
           +FD AYGP WHCIVGTSFGS+VTHS GGFLY  +D K+YVLLFKT+V+  D
Sbjct: 81  DFDSAYGPAWHCIVGTSFGSYVTHSTGGFLYFQID-KVYVLLFKTAVEPLD 127

[Close]

BLAST of Carg06694 vs. TAIR 10
Match: AT1G52240.2 (RHO guanyl-nucleotide exchange factor 11 )

HSP 1 Score: 72.0 bits (175), Expect = 6.3e-13
Identity = 35/79 (44.30%), Postives = 51/79 (64.56%), Query Frame = 0


Query: 124 DMPPAMQIHAVDYARKAHDSMEKFTSKALALSLKREFDGAYGPVWHCIVGTSFGSFVTHS 183
           DMP  MQ+ A+ +A +A D  + F  K++A  +K+EFD  YG  W C+VG++FG F THS
Sbjct: 12  DMPVKMQMQAMAFASQALDLFDVFDCKSIAGHIKKEFDERYGSGWQCVVGSNFGCFFTHS 71

Query: 184 IGGFLYLSMDQKLYVLLFK 203
            G F+Y  + + L  L+FK
Sbjct: 72  KGTFIYFQL-ETLKFLIFK 89

[Close]

BLAST of Carg06694 vs. TAIR 10
Match: AT3G16120.1 (Dynein light chain type 1 family protein )

HSP 1 Score: 72.0 bits (175), Expect = 6.3e-13
Identity = 37/84 (44.05%), Postives = 51/84 (60.71%), Query Frame = 0


Query: 119 KVVSVDMPPAMQIHAVDYARKAHDSMEKFTSKALALSLKREFDGAYGPVWHCIVGTSFGS 178
           KV   DMP  MQ+ A+  A ++ D  + F S ++A  +K+EFD  YG  W C+VGT+FG 
Sbjct: 7   KVEETDMPVKMQMQAMKIASQSLDLFDVFDSISIAAHIKKEFDERYGSGWQCVVGTNFGC 66

Query: 179 FVTHSIGGFLYLSMDQKLYVLLFK 203
           F THS G F+Y  +   L  L+FK
Sbjct: 67  FFTHSKGTFIYFHLG-TLNFLIFK 89

[Close]

BLAST of Carg06694 vs. TAIR 10
Match: AT4G15930.1 (Dynein light chain type 1 family protein )

HSP 1 Score: 71.2 bits (173), Expect = 1.1e-12
Identity = 42/99 (42.42%), Postives = 60/99 (60.61%), Query Frame = 0


Query: 106 QVELADIFAKNGIKVVSVDMPPAMQIHAVDYARKAHDSMEKFT-SKALALSLKREFDGAY 165
           +VE +    K  + + S DM   MQ  A++ A  A    EK++  K +A ++K+EFD  +
Sbjct: 29  EVEASPPAGKRAV-IKSADMKDDMQKEAIEIAISA---FEKYSVEKDIAENIKKEFDKKH 88

Query: 166 GPVWHCIVGTSFGSFVTHSIGGFLYLSMDQKLYVLLFKT 204
           G  WHCIVG +FGS+VTH    F+Y  +DQK  VLLFK+
Sbjct: 89  GATWHCIVGRNFGSYVTHETNHFVYFYLDQKA-VLLFKS 122

The following BLAST results are available for this feature:

BLAST of Carg06694 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6573027.1	1.4e-110	100.00	hypothetical protein SDJN03_26914, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022954796.1	2.9e-108	98.09	uncharacterized protein LOC111456948 [Cucurbita moschata] >XP_022954797.1 unchar...	[more]
XP_022994662.1	2.6e-101	92.82	uncharacterized protein LOC111490306 isoform X2 [Cucurbita maxima]	[more]
XP_023541664.1	1.4e-99	91.87	uncharacterized protein LOC111801755 [Cucurbita pepo subsp. pepo] >XP_023541665....	[more]
XP_022994659.1	2.1e-98	87.00	uncharacterized protein LOC111490306 isoform X1 [Cucurbita maxima] >XP_022994660...	[more]

BLAST of Carg06694 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O02414	3.1e-12	48.24	Dynein light chain LC6, flagellar outer arm OS=Heliocidaris crassispina OX=10431...	[more]
Q86A88	6.8e-12	47.06	Dynein light chain, cytoplasmic OS=Dictyostelium discoideum OX=44689 GN=dlcB PE=...	[more]
Q3MHR3	3.4e-11	45.88	Dynein light chain 2, cytoplasmic OS=Bos taurus OX=9913 GN=DYNLL2 PE=3 SV=1	[more]
Q96FJ2	3.4e-11	45.88	Dynein light chain 2, cytoplasmic OS=Homo sapiens OX=9606 GN=DYNLL2 PE=1 SV=1	[more]
Q9D0M5	3.4e-11	45.88	Dynein light chain 2, cytoplasmic OS=Mus musculus OX=10090 GN=Dynll2 PE=1 SV=1	[more]

BLAST of Carg06694 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GRW3	1.4e-108	98.09	uncharacterized protein LOC111456948 OS=Cucurbita moschata OX=3662 GN=LOC1114569...	[more]
A0A6J1JWH6	1.3e-101	92.82	uncharacterized protein LOC111490306 isoform X2 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1K3H0	1.0e-98	87.00	uncharacterized protein LOC111490306 isoform X1 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1E7P6	9.4e-65	55.21	uncharacterized protein LOC111431561 OS=Cucurbita moschata OX=3662 GN=LOC1114315...	[more]
A0A6J1KET7	8.8e-63	54.70	uncharacterized protein LOC111495174 OS=Cucurbita maxima OX=3661 GN=LOC111495174...	[more]

BLAST of Carg06694 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G20110.1	1.0e-26	62.64	Dynein light chain type 1 family protein	[more]
AT1G23220.1	8.2e-21	49.55	Dynein light chain type 1 family protein	[more]
AT1G52240.2	6.3e-13	44.30	RHO guanyl-nucleotide exchange factor 11	[more]
AT3G16120.1	6.3e-13	44.05	Dynein light chain type 1 family protein	[more]
AT4G15930.1	1.1e-12	42.42	Dynein light chain type 1 family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001372	Dynein light chain, type 1/2	SMART	SM01375	Dynein_light_2	coord: 113..203 e-value: 3.2E-34 score: 129.7
IPR001372	Dynein light chain, type 1/2	PFAM	PF01221	Dynein_light	coord: 118..203 e-value: 1.2E-26 score: 92.9
IPR001372	Dynein light chain, type 1/2	PANTHER	PTHR11886	DYNEIN LIGHT CHAIN	coord: 66..209
IPR037177	Dynein light chain superfamily	GENE3D	3.30.740.10	Protein Inhibitor Of Neuronal Nitric Oxide Synthase;	coord: 112..204 e-value: 2.2E-28 score: 100.1
IPR037177	Dynein light chain superfamily	SUPERFAMILY	54648	DLC	coord: 116..203
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..46
None	No IPR available	PANTHER	PTHR11886:SF80	INTRASTRAND CROSS-LINK RECOGNITION PROTEIN-LIKE	coord: 66..209

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg06694-RA	Carg06694-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0007017	microtubule-based process
cellular_component	GO:0030286	dynein complex
molecular_function	GO:0045505	dynein intermediate chain binding
molecular_function	GO:0051959	dynein light intermediate chain binding